rna polymerase i

Summary

Summary: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin. EC 2.7.7.6.

Top Publications

  1. ncbi The RNA polymerase I transcription machinery: an emerging target for the treatment of cancer
    Denis Drygin
    Cylene Pharmaceuticals, San Diego, California, USA
    Annu Rev Pharmacol Toxicol 50:131-56. 2010
  2. ncbi Nuclear actin and myosin I are required for RNA polymerase I transcription
    Vlada V Philimonenko
    Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic
    Nat Cell Biol 6:1165-72. 2004
  3. ncbi Conservation between the RNA polymerase I, II, and III transcription initiation machineries
    Alessandro Vannini
    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 45:439-46. 2012
  4. ncbi c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I
    Carla Grandori
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Nat Cell Biol 7:311-8. 2005
  5. pmc UBF binding in vivo is not restricted to regulatory sequences within the vertebrate ribosomal DNA repeat
    Audrey C O'Sullivan
    Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom
    Mol Cell Biol 22:657-68. 2002
  6. pmc UBF levels determine the number of active ribosomal RNA genes in mammals
    Elaine Sanij
    Research Division, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia
    J Cell Biol 183:1259-74. 2008
  7. pmc Inhibition of RNA polymerase I as a therapeutic strategy to promote cancer-specific activation of p53
    Megan J Bywater
    Division of Cancer Research, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria 3002, Australia
    Cancer Cell 22:51-65. 2012
  8. pmc Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination
    Bernd Schimanski
    Department of Genetic and Developmental Biology, University of Connecticut Health Center, 263 Farmington Ave, Farmington, CT 06030 3301, USA
    Eukaryot Cell 4:1942-50. 2005
  9. ncbi CSB is a component of RNA pol I transcription
    John Bradsher
    Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, F 67404, Illkirch Cedex, France
    Mol Cell 10:819-29. 2002
  10. pmc RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
    Benjamin Albert
    Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Universite de Toulouse, F 31000 Toulouse, France
    J Cell Biol 192:277-93. 2011

Research Grants

Detail Information

Publications263 found, 100 shown here

  1. ncbi The RNA polymerase I transcription machinery: an emerging target for the treatment of cancer
    Denis Drygin
    Cylene Pharmaceuticals, San Diego, California, USA
    Annu Rev Pharmacol Toxicol 50:131-56. 2010
    The RNA polymerase I (Pol I) transcription machinery in the nucleolus is the key convergence point that collects and integrates a vast array of information from cellular signaling cascades to regulate ribosome production that in turn ..
  2. ncbi Nuclear actin and myosin I are required for RNA polymerase I transcription
    Vlada V Philimonenko
    Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic
    Nat Cell Biol 6:1165-72. 2004
    ..Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription...
  3. ncbi Conservation between the RNA polymerase I, II, and III transcription initiation machineries
    Alessandro Vannini
    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 45:439-46. 2012
    ..This review outlines the similarities and differences between these important molecular machines...
  4. ncbi c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I
    Carla Grandori
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Nat Cell Biol 7:311-8. 2005
    ..We propose that stimulation of rRNA synthesis by c-Myc is a key pathway driving cell growth and tumorigenesis...
  5. pmc UBF binding in vivo is not restricted to regulatory sequences within the vertebrate ribosomal DNA repeat
    Audrey C O'Sullivan
    Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom
    Mol Cell Biol 22:657-68. 2002
    ..protein UBF binds to the promoter of vertebrate ribosomal repeats and is required for their transcription by RNA polymerase I in vitro...
  6. pmc UBF levels determine the number of active ribosomal RNA genes in mammals
    Elaine Sanij
    Research Division, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia
    J Cell Biol 183:1259-74. 2008
    ..regulating the number of active copies of the approximately 200 ribosomal RNA (rRNA) genes transcribed by RNA polymerase I are unclear...
  7. pmc Inhibition of RNA polymerase I as a therapeutic strategy to promote cancer-specific activation of p53
    Megan J Bywater
    Division of Cancer Research, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria 3002, Australia
    Cancer Cell 22:51-65. 2012
    Increased transcription of ribosomal RNA genes (rDNA) by RNA Polymerase I is a common feature of human cancer, but whether it is required for the malignant phenotype remains unclear...
  8. pmc Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination
    Bernd Schimanski
    Department of Genetic and Developmental Biology, University of Connecticut Health Center, 263 Farmington Ave, Farmington, CT 06030 3301, USA
    Eukaryot Cell 4:1942-50. 2005
    ..purified and characterized the U1 small nuclear ribonucleoprotein particle, the transcription factor complex TATA-binding protein related factor 4 (TRF4)/SNAPc/transcription factor IIA (TFIIA), and RNA polymerase I of Trypanosoma brucei.
  9. ncbi CSB is a component of RNA pol I transcription
    John Bradsher
    Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, F 67404, Illkirch Cedex, France
    Mol Cell 10:819-29. 2002
    ..In addition to revealing an unanticipated function for CSB in rRNA synthesis, we show that the fragility of this complex could be one factor contributing to the CS phenotype...
  10. pmc RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
    Benjamin Albert
    Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Universite de Toulouse, F 31000 Toulouse, France
    J Cell Biol 192:277-93. 2011
    b>RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs)...
  11. ncbi A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei
    M Navarro
    Present address Instituto de Parasitología y Biomedicina López Neyra, CSIC, C Ventanilla 11, 18001 Granada, Spain
    Nature 414:759-63. 2001
    ..Here we identify an extranucleolar body containing RNA polymerase I (pol I) that is transcriptionally active and present only in the bloodstream form of the parasite...
  12. pmc Nuclear myosin I acts in concert with polymeric actin to drive RNA polymerase I transcription
    Jing Ye
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Genes Dev 22:322-30. 2008
    ..Here we show that actin and NM1 act in concert to promote RNA polymerase I (Pol I) transcription...
  13. pmc RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation
    DAVID ALAN SCHNEIDER
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 720 20th Street South, Kaul Human Genetics, Room 442, Birmingham, AL 35294, USA
    Gene 493:176-84. 2012
    ..Many studies have shown that transcription of the ribosomal DNA by RNA polymerase I is a major target for cellular regulation of ribosome synthesis rates...
  14. pmc MCM-BP is required for repression of life-cycle specific genes transcribed by RNA polymerase I in the mammalian infectious form of Trypanosoma brucei
    Hee Sook Kim
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 8:e57001. 2013
    ..and procyclin-associated genes (PAGs), that are normally repressed in BF trypanosomes and are transcribed by RNA polymerase I. Our study uncovers a functional link between chromosome maintenance and RNA pol I-mediated gene silencing in ..
  15. pmc mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability
    Christine Mayer
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Genes Dev 18:423-34. 2004
    In cycling cells, transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with cell growth...
  16. pmc RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components
    Jennifer E G Gallagher
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520 8024, USA
    Genes Dev 18:2506-17. 2004
    ..This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome...
  17. pmc TDP1 is an HMG chromatin protein facilitating RNA polymerase I transcription in African trypanosomes
    Mani Shankar Narayanan
    Division of Cell and Molecular Biology, Imperial College London, South Kensington, London SW7 2AZ, UK
    Nucleic Acids Res 41:2981-92. 2013
    ..transcribes its variant surface glycoprotein (VSG) gene expression sites (ESs) in a monoallelic fashion using RNA polymerase I (Pol I). It is still unclear how ES transcription is controlled in T. brucei...
  18. pmc Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription
    Rong Cong
    Universite de Lyon, Ecole Normale Superieure de Lyon, CNRS USR 3010, Laboratoire Joliot Curie, 69364 Lyon, France
    Nucleic Acids Res 40:9441-54. 2012
    ..required for ribosomal RNA gene (rRNA) transcription in vivo, but the mechanism by which nucleolin modulates RNA polymerase I (RNAPI) transcription is not well understood...
  19. ncbi c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription
    Azadeh Arabi
    Södertörns högskola, S 141 89 Huddinge, Sweden
    Nat Cell Biol 7:303-10. 2005
    ..cells and is followed by recruitment of the Myc cofactor TRRAP, enhanced histone acetylation, recruitment of RNA polymerase I (Pol I), and activation of rDNA transcription...
  20. pmc 1A6/DRIM, a novel t-UTP, activates RNA polymerase I transcription and promotes cell proliferation
    Qunhui Peng
    Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Peking University School of Oncology, Beijing Cancer Hospital and Institute, Beijing, China
    PLoS ONE 5:e14244. 2010
    ..Ribosome subunits are assembled in the nucleolus following transcription of a 47S ribosome RNA precursor by RNA polymerase I and rRNA processing to produce mature 18S, 28S and 5.8S rRNAs...
  21. ncbi Wisely chosen paths--regulation of rRNA synthesis: delivered on 30 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden
    Ingrid Grummt
    Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    FEBS J 277:4626-39. 2010
    ..I will survey our present knowledge of the highly coordinated networks that regulate transcription by RNA polymerase I, coordinating rRNA gene transcription and ribosome production with environmental cues...
  22. pmc FACT facilitates chromatin transcription by RNA polymerases I and III
    Joanna L Birch
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    EMBO J 28:854-65. 2009
    ..Our data also imply that local chromatin dynamics influence transcription of the active rRNA genes by Pol I and of Pol III-transcribed genes...
  23. pmc Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination
    Junya Kawauchi
    Sir William Dunn School of Pathology, Oxford OX1 3RE, United Kingdom
    Genes Dev 22:1082-92. 2008
    Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination...
  24. pmc The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription
    Piergiorgio Percipalle
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Box 285, Stockholm 171 77, Sweden
    EMBO Rep 7:525-30. 2006
    ..NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA)...
  25. pmc Nucleosomes are depleted at the VSG expression site transcribed by RNA polymerase I in African trypanosomes
    Luisa M Figueiredo
    Laboratory of Molecular Parasitology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Eukaryot Cell 9:148-54. 2010
    In most eukaryotes, RNA polymerase I (Pol I) exclusively transcribes long arrays of identical rRNA genes (ribosomal DNA [rDNA])...
  26. ncbi The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks
    Michael Kruhlak
    Experimental Immunology, NCI
    Nature 447:730-4. 2007
    ..Here we monitor RNA polymerase I (Pol I) activity in mouse cells exposed to genotoxic stress and show that induction of DNA breaks leads to a ..
  27. ncbi Establishment and maintenance of alternative chromatin states at a multicopy gene locus
    Manuel Wittner
    Lehrstuhl Biochemie III, Universitat Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany
    Cell 145:543-54. 2011
    In eukaryotes, each of the more than 100 copies of ribosomal RNA (rRNA) genes exists in either an RNA polymerase I transcribed open chromatin state or a nucleosomal, closed chromatin state...
  28. ncbi At the crossroads of growth control; making ribosomal RNA
    Tom Moss
    Cancer Research Centre and Department of Medical Biology, Laval University, Hotel Dieu de Quebec, 9 rue McMahon, G1R 2J6 Québec, Canada
    Curr Opin Genet Dev 14:210-7. 2004
    ..In the past few years we have begun to penetrate the network linking rRNA gene transcription to growth...
  29. pmc Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system
    Olivier Gadal
    Laboratoire de Physio Génomique, Service de Biochimie et Génétique Moléculaire, CEA Saclay, Batiment 142, F 91191 Gif sur Yvette, France
    EMBO J 21:5498-507. 2002
    ..Hmo1 overexpression suppresses rpa49-Delta mutants lacking Rpa49, a non-essential but conserved subunit of RNA polymerase I corresponding to the animal RNA polymerase I factor PAF53...
  30. ncbi Cdc14 inhibits transcription by RNA polymerase I during anaphase
    Andres Clemente-Blanco
    Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 0NN, UK
    Nature 458:219-22. 2009
    ..The phosphatase activity of Cdc14 is required for RNA polymerase I (Pol I) inhibition in vitro and in vivo...
  31. pmc mTOR binds to the promoters of RNA polymerase I- and III-transcribed genes
    Chi Kwan Tsang
    Department of Pharmacology and Cancer Institute of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Cell Cycle 9:953-7. 2010
    ..our observations suggest that mTOR is closely involved in gene regulation at the promoters, which is a conserved mechanism to control RNA polymerase I- and III-dependent genes that are critical for protein synthesis and cell growth.
  32. pmc Efficient termination of transcription by RNA polymerase I requires the 5' exonuclease Rat1 in yeast
    Aziz El Hage
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genes Dev 22:1069-81. 2008
    ..We report that the activity of Rat1 is also required for efficient termination by RNA polymerase I (Pol I) on the rDNA...
  33. pmc Transcription-dependent recombination and the role of fork collision in yeast rDNA
    Yasushi Takeuchi
    National Institute for Basic Biology, Myodaijicho, Okazaki 444 8585, Japan
    Genes Dev 17:1497-506. 2003
    ..The collision was dependent on the transcription by RNA polymerase I. In addition, the transcription stimulated rDNA copy number variation, and the production of the ..
  34. ncbi Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I
    Yohsuke Ogawa
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Gen Virol 88:3385-90. 2007
    ..In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase...
  35. ncbi Silencing in yeast rDNA chromatin: reciprocal relationship in gene expression between RNA polymerase I and II
    Francesco Cioci
    Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
    Mol Cell 12:135-45. 2003
    ..Together with the demonstration of a requirement for active Pol I in silencing, these results show a reciprocal relationship in gene expression between Pol I and Pol II in rDNA...
  36. pmc Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells
    Natalia Shcherbik
    Department of Cell Biology, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, New Jersey 08084, USA
    EMBO Rep 11:106-11. 2010
    ..Here, we show that in mammals, aberrant pre-rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (..
  37. pmc Repression of RNA polymerase I transcription by the tumor suppressor p53
    W Zhai
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Mol Cell Biol 20:5930-8. 2000
    ..In summary, our results suggest that p53 represses RNA Pol I transcription by directly interfering with the assembly of a productive transcriptional machinery on the rRNA promoter...
  38. pmc Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription
    Yuan Chin Tsai
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Mol Cell Proteomics 11:60-76. 2012
    ....
  39. ncbi Constitutive and strong association of PAF53 with RNA polymerase I
    P Seither
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Chromosoma 106:216-25. 1997
    Mouse RNA polymerase I (Pol I) is composed of 14 polypeptides, 3 of which are thought to be loosely associated with, and may be dislodged from, Pol I...
  40. pmc Nucleolar assembly of the rRNA processing machinery in living cells
    T M Savino
    Institut Jacques Monod, UMR 7592, 2 place Jussieu, 75251 Paris Cedex 05, France
    J Cell Biol 153:1097-110. 2001
    ..This demonstrates the formation of one functional domain by cooperative interactions between different chromosome territories...
  41. pmc The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively
    Susan J Anderson
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    J Biol Chem 286:18816-24. 2011
    ..and WT strains confirms these observations and supports the model that Spt4/5 may contribute to pausing of RNA polymerase I early during transcription elongation but promotes transcription elongation downstream of the pause(s)...
  42. pmc Transcription elongation by RNA polymerase I is linked to efficient rRNA processing and ribosome assembly
    David A Schneider
    Department of Biological Chemistry, University of California, Irvine, 240 D Medical Sciences I, Irvine, CA 92697 1700, USA
    Mol Cell 26:217-29. 2007
    ..Here, we describe the identification of a point mutation in the second largest subunit of RNA polymerase I near the active center of the enzyme that results in an elongation-defective enzyme in the yeast ..
  43. ncbi Growth factor signaling regulates elongation of RNA polymerase I transcription in mammals via UBF phosphorylation and r-chromatin remodeling
    Victor Stefanovsky
    Cancer Research Centre and Department of Medical Biology, Laval University, Hotel Dieu de Quebec, 11 Cote du Palais, G1R 2J6 Québec, Canada
    Mol Cell 21:629-39. 2006
    Synthesis of the 45S rRNA by RNA polymerase I limits cell growth. Knowledge of the mechanism of its regulation is therefore key to understanding growth control...
  44. pmc Acetylation of TAF(I)68, a subunit of TIF-IB/SL1, activates RNA polymerase I transcription
    V Muth
    Division of Molecular Biology of the Cell II, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    EMBO J 20:1353-62. 2001
    ..Moreover, PCAF stimulates RNA polymerase I (Pol I) transcription in a reconstituted in vitro system...
  45. pmc Display of VP1 on the surface of baculovirus and its immunogenicity against heterologous human enterovirus 71 strains in mice
    Tao Meng
    Animal Health Biotechnology, Temasek Life Sciences Laboratory, Republic of Singapore
    PLoS ONE 6:e21757. 2011
    ..VP1 is one of the major immunogenic capsid protein of EV71 and plays a crucial role in viral infection. Antibodies against VP1 are important for virus neutralization...
  46. pmc TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei
    Tara M Stanne
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London South Kensington, Imperial College Road, London, United Kingdom
    Eukaryot Cell 10:964-76. 2011
    ..Our results indicate that TbISWI is a versatile chromatin remodeler that regulates transcription at multiple Pol I loci and is particularly abundant at many Pol II transcription boundaries in T. brucei...
  47. pmc TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
    Anja Philippi
    Institut fur Biochemie, Genetik und Mikrobiologie, Universitat Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 38:5315-26. 2010
    ..step in the regulation of ribosomal RNA (rRNA) gene transcription is the formation of the complex between RNA polymerase I (Pol I) and the Pol I-dependent transcription factor Rrn3p...
  48. pmc Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression
    Kalpana Ghoshal
    Department of Molecular and Cellular Biochemistry, College of Medicine, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:6783-93. 2004
    ..To study the effect of CpG methylation on RNA polymerase I (pol I)-transcribed rRNA genes, we constructed pHrD-IRES-Luc (human rRNA promoter-luciferase reporter)...
  49. pmc Tor pathway regulates Rrn3p-dependent recruitment of yeast RNA polymerase I to the promoter but does not participate in alteration of the number of active genes
    Jonathan A Claypool
    Department of Biological Chemistry, University of California, Irvine, Irvine, California 92697 1700, USA
    Mol Biol Cell 15:946-56. 2004
    ..Using chromatin immunoprecipitation assays, we found that the association of RNA polymerase I with the promoter and the coding region of rDNA is decreased in stationary phase, but association of ..
  50. pmc Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription
    Takahiro Masaki
    Department of Virology II, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    J Virol 84:5824-35. 2010
    In this study, we used an RNA polymerase I (Pol I) transcription system for development of a reverse genetics protocol to produce hepatitis C virus (HCV), which is an uncapped positive-strand RNA virus...
  51. pmc The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components
    Arnaud Laferté
    Laboratoire de Transcription des Gènes, Service de Biochimie et de Génétique Moléculaire, CEA Saclay, F Gif sur Yvette, France
    Genes Dev 20:2030-40. 2006
    ....
  52. ncbi Structure-function analysis of RNA polymerases I and III
    Michel Werner
    CEA, IRCM, F 91057 Evry Cedex, France
    Curr Opin Struct Biol 19:740-5. 2009
    ..Unexpectedly, even though Pol I and III, but not Pol II, have an intrinsic RNA cleavage activity, it was found that TFIIS Pol II cleavage stimulation factor also played a general role in Pol III transcription...
  53. ncbi RNA polymerase I contains a TFIIF-related DNA-binding subcomplex
    Sebastian R Geiger
    Gene Center and Department of Biochemistry, Center for Integrated Protein Science CIPSM, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 39:583-94. 2010
    ..Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity...
  54. pmc In exponentially growing Saccharomyces cerevisiae cells, rRNA synthesis is determined by the summed RNA polymerase I loading rate rather than by the number of active genes
    Sarah L French
    Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia 22908 0734, USA
    Mol Cell Biol 23:1558-68. 2003
    ..Results also allow an estimate of elongation rate of approximately 60 nucleotides/s for yeast Pol I and a reinitiation rate of less than 1 s on the most heavily transcribed genes...
  55. ncbi The nucleolus and transcription of ribosomal genes
    Ivan Raska
    1st Faculty of Medicine, Charles University and Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 12800 Prague 2, Czech Republic
    Biol Cell 96:579-94. 2004
    ..In this review, we discuss the recent results on the regulation of rRNA synthesis in relation to the functional organization of the nucleolus, and put an emphasis on the situation encountered in mammalian somatic cells...
  56. pmc Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription
    R Voit
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 98:13631-6. 2001
    ..interaction studies reveal that phosphorylation at serine 388 is required for the interaction between RNA polymerase I and UBF...
  57. pmc Upstream binding factor association induces large-scale chromatin decondensation
    Danyang Chen
    Department of Cell and Molecular Biology, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:15106-11. 2004
    ..These studies also provide an in vivo model simulating ribosomal DNA transactivation outside the nucleolus, allowing temporal and spatial analyses of chromatin remodeling and assembly of the pol I transcription machinery...
  58. pmc UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting sequences
    M Scott
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    Nucleic Acids Res 29:2052-8. 2001
    ..Taken together, these results indicate that UV induces ING1 to translocate to the nucleolus and that this translocation may facilitate apoptosis...
  59. ncbi Activity of RNA polymerase I transcription factor UBF blocked by Rb gene product
    A H Cavanaugh
    Sigfried and Janet Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822 2618
    Nature 374:177-80. 1995
    ..of rDNA transcription; (2) addition of Rb to an in vitro transcription system inhibits transcription by RNA polymerase I; (3) this inhibition requires a functional Rb pocket; and (4) Rb specifically inhibits the activity of the ..
  60. ncbi RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA
    Hannah S Jones
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Struct Mol Biol 14:123-30. 2007
    ..We suggest that Pol I transcription, like that of Pol II, may be modulated by specific chromatin structures...
  61. pmc Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I
    Y Nogi
    Department of Biological Chemistry, University of California, Irvine 92717
    Proc Natl Acad Sci U S A 88:3962-6. 1991
    ..on a multicopy plasmid and induced by galactose, suppressed the growth defects of a temperature-sensitive RNA polymerase I (pol I) mutant and those of a mutant in which the gene for the second largest subunit of pol I was deleted...
  62. ncbi When rDNA transcription is arrested during mitosis, UBF is still associated with non-condensed rDNA
    J Gebrane-Younes
    Institut Jacques Monod, Paris, France
    J Cell Sci 110:2429-40. 1997
    ..It is proposed that modifications of the transcription machinery occurring in prometaphase could explain the arrest of transcription, while reverse modifications in late anaphase could explain activation...
  63. pmc BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription
    Patrick M Grierson
    Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University College of Medicine, Columbus, OH 43210 2207, USA
    Hum Mol Genet 21:1172-83. 2012
    ..lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription...
  64. ncbi The ARF tumor suppressor controls ribosome biogenesis by regulating the RNA polymerase I transcription factor TTF-I
    Frédéric Lessard
    Cancer Research Centre, Department of Molecular Biology, Medical Biochemistry, and Pathology of Laval University, CHUQ Research Centre, Quebec, Quebec, Canada
    Mol Cell 38:539-50. 2010
    ..We show that ARF does this by controlling the subnuclear localization of the RNA polymerase I transcription termination factor, TTF-I...
  65. pmc RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template
    R T Yamamoto
    Department of Biological Chemistry, University of California, Irvine, CA 92717, USA
    EMBO J 15:3964-73. 1996
    RRN3 is one of the RRN genes specifically required for the transcription of rDNA by RNA polymerase I (Pol I) in Saccharomyces cerevisiae...
  66. ncbi Targeting RNA polymerase I with an oral small molecule CX-5461 inhibits ribosomal RNA synthesis and solid tumor growth
    Denis Drygin
    Cylene Pharmaceuticals, Inc, San Diego, California, USA
    Cancer Res 71:1418-30. 2011
    ..Our findings position CX-5461 for investigational clinical trials as a potent, selective, and orally administered agent for cancer treatment...
  67. ncbi Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins
    H M Jantzen
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Nature 344:830-6. 1990
    ..binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1...
  68. ncbi Treacle recruits RNA polymerase I complex to the nucleolus that is independent of UBF
    Chen I Lin
    Institute of Microbiology and Immunology, School of Life Science, National Yang Ming University, Taipei 11221, Taiwan, ROC
    Biochem Biophys Res Commun 386:396-401. 2009
    ..Here we show that the central repeated domain of treacle binds with RNA polymerase I (Pol I), while that the treacle C-terminus is involved in rDNA promoter recognition and UBF recruitment...
  69. pmc Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae
    Yuko Todaka
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Genetics 170:1515-24. 2005
    ..Here, we propose that Gtr1p is involved in RNA polymerase I and III assembly by its association with Rpc19p and could be a mediator that links growth regulatory signals ..
  70. ncbi Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a
    Xuejun Yuan
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Mol Cell 27:585-95. 2007
    ..Here we show that a complex consisting of CSB, RNA polymerase I (Pol I), and histone methyltransferase G9a is present at active rRNA genes...
  71. ncbi Structure and function of the nucleolus in the spotlight
    Ivan Raska
    Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Czech Republic
    Curr Opin Cell Biol 18:325-34. 2006
    ..However, the present power of light and electron microscopy techniques means that a description of the organization of nucleolar processes at the molecular level is now achievable, and the time is ripe for such an effort...
  72. pmc The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3
    G Peyroche
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, F 91191 Gif sur Yvette Cedex, France
    EMBO J 19:5473-82. 2000
    b>RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood...
  73. pmc Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production
    Shin Murakami
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Virol 82:1605-9. 2008
    ....
  74. pmc A targeting modality for destruction of RNA polymerase I that possesses anticancer activity
    Karita Peltonen
    Molecular Cancer Biology Program and Centre for Drug Research, University of Helsinki, Helsinki 00014, Finland
    Cancer Cell 25:77-90. 2014
    ..sequences, which are present at a high frequency in ribosomal DNA genes, and potently and rapidly represses RNA polymerase I (Pol I) transcription...
  75. ncbi Is cell size important?
    Renato Baserga
    Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cell Cycle 6:814-6. 2007
    ..Cell size is largely determined by the activity of RNA polymerase I that controls ribosomal RNA synthesis and ribosome biogenesis...
  76. pmc RNA polymerase I-driven minigenome system for Ebola viruses
    Allison Groseth
    National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
    J Virol 79:4425-33. 2005
    ..roles for transcription and replication in the reduced pathogenicity of Reston ebolavirus, we developed an RNA polymerase I (Pol I)-driven minigenome system...
  77. ncbi Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex
    Andrew J Lindsay
    Molecular Cell Biology Laboratory, Department of Biochemistry, Biosciences Institute, University College Cork, Cork, Ireland
    Cell Motil Cytoskeleton 66:1057-72. 2009
    ..Myosin Vb was present in nucleoli where it co-localises with RNA polymerase I, and newly synthesised ribosomal RNA (rRNA), indicating that it may play a role in transcription...
  78. ncbi RNA polymerase I-mediated transcription of a reporter gene integrated into different loci of Leishmania
    Nathalie Boucher
    Faculte de Medecine, Centre de Recherche en Infectiologie, Centre hospitalier de l Universite Laval, Universite Laval, Pavillon CHUL, 2705 Boul Laurier, Ste Foy, G1V 4G2, Quebec, Canada
    Mol Biochem Parasitol 119:153-8. 2002
  79. pmc hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF)
    RuiRui Kong
    Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Peking University School of Oncology, Beijing Cancer Hospital and Institute, Beijing 100142, China
    J Biol Chem 286:7139-48. 2011
    ..UBF depending on HAT in vivo, and hALP but not hALP (ΔHAT) facilitated the nuclear translocation of the RNA polymerase I (Pol I)-associated factor 53 (PAF53) from the cytoplasm and promoted the association of UBF with PAF53...
  80. pmc Integrative genomic analysis of human ribosomal DNA
    Gabriel E Zentner
    Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA
    Nucleic Acids Res 39:4949-60. 2011
    ..Taken together, these analyses confirm and expand the results of previous ChIP studies of rDNA and provide novel avenues for exploration of chromatin-mediated regulation of rDNA...
  81. pmc The chromatin remodeling complex NuRD establishes the poised state of rRNA genes characterized by bivalent histone modifications and altered nucleosome positions
    Wenbing Xie
    the Education Ministry, Key Laboratory of Cell Proliferation and Differentiation, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 109:8161-6. 2012
    ..The results uncover a unique mechanism by which ATP-dependent chromatin remodeling complexes with opposing activities establish a specific chromatin state and regulate transcription...
  82. pmc DNA sequence encoded repression of rRNA gene transcription in chromatin
    Max Felle
    Institut für Biochemie III, Universitat Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 38:5304-14. 2010
    ..activation as specific inhibition of ATP-dependent chromatin remodelling suppresses the initiation of RNA Polymerase I transcription in vitro...
  83. ncbi RNA polymerase I propagates unidirectional spreading of rDNA silent chromatin
    Stephen W Buck
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
    Cell 111:1003-14. 2002
    ..we demonstrate that rDNA silencing spreads into the centromere-proximal unique sequence located downstream of RNA polymerase I (Pol I) transcription, but fails to enter the upstream telomere-proximal sequences...
  84. pmc A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding protein to promote polymerase I transcription initiation in Saccharomyces cerevisiae
    C W Lin
    Basic Sciences Division, Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 16:6436-43. 1996
    We report the cloning of RRN11, a gene coding for a 66-kDa protein essential for transcription initiation by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae...
  85. pmc Reverse genetics system for Uukuniemi virus (Bunyaviridae): RNA polymerase I-catalyzed expression of chimeric viral RNAs
    R Flick
    Ludwig Institute for Cancer Research, Stockholm Branch, Karolinska Institute, S 17177 Stockholm, Sweden
    J Virol 75:1643-55. 2001
    ..a reverse genetics system for the phlebovirus Uukuniemi virus, a member of the Bunyaviridae family, by using RNA polymerase I (pol I)-mediated transcription...
  86. pmc Evolution of two modes of intrinsic RNA polymerase transcript cleavage
    Wenjie Ruan
    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    J Biol Chem 286:18701-7. 2011
    ....
  87. pmc A TBP-containing multiprotein complex (TIF-IB) mediates transcription specificity of murine RNA polymerase I
    D Eberhard
    Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg
    Nucleic Acids Res 21:4180-6. 1993
    ..with the mouse ribosomal gene promoter and nucleates the formation of an initiation complex containing RNA polymerase I (Pol I)...
  88. pmc The Paf1 complex is required for efficient transcription elongation by RNA polymerase I
    Yinfeng Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, AL 35294 0024, USA
    Proc Natl Acad Sci U S A 106:2153-8. 2009
    Regulation of RNA polymerase I (Pol I) transcription is critical for controlling ribosome synthesis. Most previous investigations into Pol I transcription regulation have focused on transcription initiation...
  89. pmc Yeast transcription elongation factor Spt5 associates with RNA polymerase I and RNA polymerase II directly
    Olga V Viktorovskaya
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    J Biol Chem 286:18825-33. 2011
    ..Based on these data, we propose a model in which Spt5 is recruited to the rDNA early in transcription and propose that it plays an important role in ribosomal RNA synthesis through direct binding to the Pol I complex...
  90. pmc RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing
    D A Schneider
    Department of Biological Chemistry, University of California, Irvine, CA 92697 1700, USA
    Proc Natl Acad Sci U S A 103:12707-12. 2006
    ..These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly...
  91. pmc The plant-specific TFIIB-related protein, pBrp, is a general transcription factor for RNA polymerase I
    Sousuke Imamura
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 27:2317-27. 2008
    ..CmpBrp was shown to co-immunoprecipitate and co-localize with the RNA polymerase I subunit, CmRPA190, in the cell...
  92. pmc The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis
    Yinfeng Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    J Biol Chem 285:14152-9. 2010
    ..rate of ribosome synthesis is proportional to the rate of cell proliferation; thus, transcription of rRNA by RNA polymerase I (Pol I) is an important target for the regulation of this process...
  93. ncbi Quantitation of GFP-fusion proteins in single living cells
    Miroslav Dundr
    National Cancer Institute, NIH, 41 Library Drive, Bldg 41, Bethesda, MD 20892 5055, USA
    J Struct Biol 140:92-9. 2002
    ..this method to estimate in single living cells the number of fluorescent transcription factor molecules on RNA polymerase I and polymerase II genes...
  94. ncbi Rpa43 and its partners in the yeast RNA polymerase I transcription complex
    Frederic Beckouët
    CEA, iBiTec S, Service de Biologie Intégrative and Génétique Moléculaire, F 91191 Gif sur Yvette, France
    FEBS Lett 585:3355-9. 2011
    An Rpa43/Rpa14 stalk protrudes from RNA polymerase I (RNAPI), with homology to Rpb7/Rpb4 (RNAPII), Rpc25/Rpc17 (RNAPIII) and RpoE/RpoF (archaea)...
  95. pmc Transcription factor UAF, expansion and contraction of ribosomal DNA (rDNA) repeats, and RNA polymerase switch in transcription of yeast rDNA
    M Oakes
    Department of Biological Chemistry, University of California, Irvine, Irvine, California 92697 1700, USA
    Mol Cell Biol 19:8559-69. 1999
    ..We discuss the significance of these observations in relation to the plasticity of rDNA tandem repeats and nucleolar structures as well as evolution of the Pol I machinery...
  96. ncbi Truncated Cockayne syndrome B protein represses elongation by RNA polymerase I
    Anton Lebedev
    Department of Dermatology and Allergic Diseases, University of Ulm, Helmholtzstr 8 1, 89081 Ulm, Germany
    J Mol Biol 382:266-74. 2008
    ..CSB protein has been shown to be a component of RNA polymerase I (Pol I) transcription...
  97. pmc Histones are required for transcription of yeast rRNA genes by RNA polymerase I
    Prasad Tongaonkar
    Department of Biological Chemistry, University of California, Irvine, CA 92697 1700, USA
    Proc Natl Acad Sci U S A 102:10129-34. 2005
    ..Thus, these results show functional importance of histones associated with actively transcribed rRNA genes...
  98. ncbi Cryo-negative staining reveals conformational flexibility within yeast RNA polymerase I
    Sacha De Carlo
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, 1 rue Laurent Fries, BP163, F 67404 Illkirch Cedex, C U de Strasbourg, France
    J Mol Biol 329:891-902. 2003
    The structure of the yeast DNA-dependent RNA polymerase I (RNA Pol I), prepared by cryo-negative staining, was studied by electron microscopy. A structural model of the enzyme at a resolution of 1...
  99. ncbi Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus
    Ingrid Grummt
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Genes Dev 17:1691-702. 2003
  100. pmc Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription
    Ethan Ford
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 20:1075-80. 2006
    ..widely expressed nucleolar protein that is associated with active rRNA genes (rDNA), where it interacts with RNA polymerase I (Pol I) as well as with histones...
  101. ncbi Rrn3 becomes inactivated in the process of ribosomal DNA transcription
    Iwona Hirschler-Laszkiewicz
    Sigfried and Janet Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17821, USA
    J Biol Chem 278:18953-9. 2003
    ..It has been suggested that the phosphorylation of either yeast RNA polymerase I or mammalian Rrn3 regulates the formation of RNA polymerase I...

Research Grants62

  1. Mode of action of a new Tat HIV-1 inhibitor
    Susana T Valente; Fiscal Year: 2013
    ..repress HIV replication in two ways: by inhibiting Tat-TAR interaction, thereby inhibiting elongation by RNA Polymerase I (Pol I) from the HIV promoter;and/or by inhibiting DNA-PK activation of the transcription factor Sp1 and ..
  2. polr1c and polr1d mutant zebrafish as new models for Treacher Collins syndrome
    KRISTIN EMILY NOACK WATT; Fiscal Year: 2013
    ..POLR1C and POLR1D encode subunits of RNA polymerase I and III, but nothing is known about the role of these genes in NC cell and craniofacial development...
  3. Visualizing Genetic Activity in Normal &Mutant Yeast
    Ann L Beyer; Fiscal Year: 2013
    ..The long-term goal of the proposed research is to better understand the molecular mechanisms that regulate RNA polymerase I transcription of ribosomal RNA...
  4. Mechanism &Function of Subnuclear Targeting of Transcription Factors in Bone
    Gary S Stein; Fiscal Year: 2011
    ..of osteoblast-specific genes (transcribed by RNA polymerase II) and ribosomal RNA genes (transcribed by RNA polymerase I) (Specific Aim 3)...
  5. GENE CONTROL IN INFECTION AND LYSOGENY BY PHAGE LAMBDA
    Jeffrey W Roberts; Fiscal Year: 2012
    ..It also directly investigates regulatory pathways related to those essential to pathogenesis by HIV and infection by toxic bacteria. ..
  6. Epigenetic regulation by TOR signaling
    RONALD LARIBEE; Fiscal Year: 2012
    ..and biochemical approaches to delineate how TOR signaling regulates a histone chaperone complex to control RNA polymerase I transcription of ribosomal DNA...
  7. Control of RNA polymerase I transcription initiation and elongation
    DAVID ALAN SCHNEIDER; Fiscal Year: 2013
    Uncontrolled growth of cancer cells is inevitably accompanied by dysregulation of RNA polymerase I activity and ribosome synthesis...
  8. Elongation and Termination of Transcripts by RNA Pol II
    DAVID LEONARD BENTLEY; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The control of gene expression through regulated transcription by RNA polymerase I (pol II) is fundamental to the cell viability and is often corrupted in disease...
  9. Functional Characterization of Nuclear Myosin I in Transcription
    Primal de Lanerolle; Fiscal Year: 2010
    ..Nuclear myosin I and actin are necessary for transcription by RNA polymerase I and II...
  10. MECHANISMS OF TCDD TOXICITY
    Diane Russell; Fiscal Year: 1990
    ..The effects of these hormones and their interactions to modify ornithine decarboxylase activity and RNA polymerase I activity in the liver of TCDD-treated rats will be assessed since a previous time-dependent effect of TCDD to ..
  11. FOOTPRINTING WITH IRON (II)-GENERATED HYDROXYL RADICAL
    THOMAS TULLIUS; Fiscal Year: 2003
    ..abstract_text> ..
  12. REGULATION OF CORNEAL EPITHELIAL RNA POLYMERASE ACTIVITY
    H Cavanagh; Fiscal Year: 1991
    ..The specific aims of the proposed research are as follows: (1) To characterize RNA polymerase I, II and III of rabbit corneal epithelium (including electrophoretic and further biochemical/kinetic ..
  13. RIBOSOMAL RNA TRANSCRIPTION FACTOR FUNCTIONAL ANALYSIS
    Marvin Paule; Fiscal Year: 1992
    ..Among the significant findings are that TIF directs RNA polymerase I to bind over the transcription start site by protein-protein contact; polymerase makes no sequence-specific ..
  14. MECHANISM OF ANTIGENIC VARIATION IN TRYPANOSOMA BRUCEI
    Keith Gottesdiener; Fiscal Year: 1992
    ..The vectors will be engineered to resemble VSG gene expression sites with which chromosomal rearrangements and transcript control of VSG genes will be studied...
  15. Molecular tools for Bunyavirus antiviral screening
    PAUL OLIVO; Fiscal Year: 2005
    ..on virus promoter-driven reporter gene constructs generated by bacteriophage T7 RNA polymerase or mammalian RNA polymerase I. Similar systems have been developed and utilized for other negative strand viruses including respiratory ..
  16. MOLECULAR GENETICS OF SINDBIS VIRUS REPLICATION
    Charles Rice; Fiscal Year: 2005
    ....
  17. CHARACTER AND ROLE OF HMG 1,2-DNA INTERACTIONS
    WILLIAM SCOVELL; Fiscal Year: 1991
    ..Different levels of alpha-amanitin will be incorporated in the assay to distinguish whether the HMG proteins exhibit any preferential effect on transcription by RNA polymerase I, II or III.
  18. Gene Amplification-Sciarid DNA Puffs
    SUSAN GERBI; Fiscal Year: 2006
    ..Information gained from the unique Sciara system should behelpful in understanding the mechanisms used by metazoa to specify an origin and to regulate its activation. ..
  19. Viral DNA Replication and Late Transcription
    Linda Guarino; Fiscal Year: 2004
    ..Knowledge gained from these experiments should be relevant to other viral systems and will provide a better understanding of the complex interactions between transcription and replication machinery in eukaryotic cells. ..
  20. BIOCHEMICAL ASPECTS OF GENE REGULATION
    MICHAEL DAHMUS; Fiscal Year: 1980
    ..The activity of highly purified Novikoff ascites tumor RNA polymerase I and II is stimulated by the presence of a purified nuclear protein kinase...
  21. STRUCTURE AND FUNCTION OF DNA BINDING PROTEINS
    JOSEPH COLEMAN; Fiscal Year: 1992
    ....
  22. NEPHRECTOMY AT BIRTH AND DURING GROWTH
    RONALD MALT; Fiscal Year: 1992
    ..mitochondrial proliferation and gene expression are components of recovery 7. effects of ischemia on renal proteins are controlled by genetic mechanisms...
  23. Analysis of rDNA Chromatin in Aging Yeast Cells
    Jeffrey Smith; Fiscal Year: 2003
    ..be tested is that aging in yeast is in part regulated by the status of the rDNA chromatin structure, and that RNA polymerase I (Pol I) activity plays a role in this process...
  24. RNA POLYMERASE I AND AUTOANTIBODIES IN MURINE SLE
    DEAN STETLER; Fiscal Year: 1992
    Autoantibodies against RNA polymerase I (RPI) have been detected in the sera of all human systemic erythematosus (SLE) patients tested...
  25. NF2 BINDING PROTEINS
    Stefan Pulst; Fiscal Year: 2000
    ..Based on nucleolar localization of schwannomin in STS26T Schwann cells, its effect on RNA polymerase I transcription will be assayed in vitro using nuclear extracts, purified transcription factors and recombinant ..
  26. Developing Murine Models of HCV Replication
    SUSAN UPRICHARD; Fiscal Year: 2004
    ..HCV can be established by transient T7-driven expression of HCV construct(s) in vivo; and 3) determine if stable RNA Polymerase I-driven expression of HCV construct(s) can serve as a chronic murine model of HCV in vivo.
  27. THE RIBOSOMAL DNA AND TRANSCRIPTIONAL SILENCING
    Jeffrey Smith; Fiscal Year: 2004
    ..These approaches will also be used to determine the role of RNA polymerase I-mediated rRNA transcription in the silencing of Pol II-transcribed genes position within the rDNA...
  28. BIOLOGY OF THE P53 PROTEIN
    Renato Baserga; Fiscal Year: 2009
    ..to the nuclei where it binds and activates UBF1 (Upstream Binding Factor 1), a protein that regulates RNA polymerase I activity, and therefore cell (and body) size...
  29. CONFERANCE ON REGULATION OF TRANSCRIPTION ELONG AND TERM
    Charles Fox; Fiscal Year: 1991
    ..Among areas of current interest are the following: 1) Termination by RNA polymerase I, mitochondrial polymerase, and possibly some polymerase II genes involves the unexpected action of a site-..
  30. HOST AND VIRAL INFLUENCES ON PHAGE LAMBDA DEVELOPMENT
    David Friedman; Fiscal Year: 2000
    ..It could play an important role in maximizing bacterial growth and thus could be a important auxiliary factor fostering pathogenesis. ..
  31. GENE REGULATION IN BACTERIAL DIFFERENTIATION
    CHARLES MORAN; Fiscal Year: 1991
    ..Furthermore, the biochemical principles that enable a protein to direct RNA polymerase to specific sites on DNA are probably relevant to RNA polymerases in all organisms...
  32. Protein interactions and initiation by RNA polymerase I
    Lawrence Rothblum; Fiscal Year: 2008
    ..One is the regulation of the ability of RNA polymerase I to initiate transcription...
  33. The Perinucleolar Compartment as Breast Cancer Marker
    Sui Huang; Fiscal Year: 2005
    ..We expect the proposed studies will clarify whether the PNC, an easily detectable cellular structure, could improve the accuracy of breast cancer prognosis and prediction when used in combination with existing markers. ..
  34. A Novel HTP Assay for Identification of Compounds that Reduce PNC Prevalence
    Sui Huang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. PERINUCLEOLAR COMPARTMENT AND CANCER
    Sui Huang; Fiscal Year: 2003
    ..The results of these experiments will provide an important foundation to address the significance of the PNC in malignant cells. ..
  36. GENETICS AND BIOCHEMISTRY OF RIBOSOME SYNTHESIS
    Masayasu Nomura; Fiscal Year: 2009
    ..is central in determining overall synthesis of ribosomes, our work focuses on regulation of rRNA synthesis by RNA Polymerase I (Pol I)...
  37. Role of RecQ Helicases to Prevent Senescence By c-Myc
    Carla Grandori; Fiscal Year: 2010
    ..If so, drug inhibitors of WRN function could be proposed for the treatment of cancer. ..
  38. Is Basonuclin essential for Oogenesis
    Hung Tseng; Fiscal Year: 2004
    ..Sub aim 1 will use an RNAi approach to reduce the amount of basonuclin in oocytes; Sub aim 2 will use the Cre recombinase to knockout [the] basonuclin gene in oocytes. ..
  39. The Role of Zinc Finger Genes in Pancreatic Cell Growth
    Raul Urrutia; Fiscal Year: 2008
    ..Project Description Page 6 Principal Investigator/Program Director (Last, first, middle): Urrutia, Raul ..
  40. Molecular mechanisms of transcription elongation
    Dmitry Vassylyev; Fiscal Year: 2009
    ..the facile translocation upon nucleotide addition, the effects of systematic variation of the scaffold components and substitutions of the key RNA polymerase residues that are highlighted by the structure will be analyzed ..
  41. Zinc metalloprotease families in Trypansoma brucei
    JOHN DONELSON; Fiscal Year: 2009
    ....
  42. The Werner syndrome protein in CPT-induced DNA damage
    Lucio Comai; Fiscal Year: 2008
    ..Taken together, these experiments should provide important mechanistic insights into the process of human aging. ..
  43. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  44. CONTROL OF RIBOSOMAL GENE TRANSCRIPTION
    RONALD REEDER; Fiscal Year: 2001
    ..Ribosomal RNA is transcribed by RNA polymerase I (po1I) and comprises over half of the total RNA of the cell...
  45. GP46 GENES AND PROTEIN EXPRESSION IN LEISHMANIA CHAGASI
    JOHN DONELSON; Fiscal Year: 2002
    ....
  46. DIRECT TARGET GENES OF THE MYC ONCOPROTEIN
    Carla Grandori; Fiscal Year: 2002
    ..These studies will provide several pieces of the puzzle necessary to understand how Myc functions as an oncogene. ..
  47. REVERSE TRANSCRIPTASE AND OTHER HIV MOLECULAR STRUCTURES
    Thomas Steitz; Fiscal Year: 2002
    ..Crystallographic studies of both full length HIV integrase and the homologous transposase, TN552, complexed with suitable DNA substrates are aimed to produce mechanistic insights. ..
  48. AUTOANTIGEN RNA HELICASE II/GU
    Benigno Valdez; Fiscal Year: 2003
    ..This study may also provide an impetus for understanding the pathological roles of RH-II/Gu on the elicitation of autoimmune responses. ..
  49. KS1, A NOVEL SUPPRESSOR OF NEOPLASTIC TRANSFORMATION
    Raul Urrutia; Fiscal Year: 2003
    ..This knowledge will serve as the foundation for future studies aimed at using these proteins as therapeutic tools for controlling abnormal cell growth. ..
  50. RNA POLYMERASE I TRANSCRIPTION & REGULATION MECHANISMS
    Marvin Paule; Fiscal Year: 2004
    ..The RNA polymerase I and the transcription factor subunits that interact with each other to mediate pol I recruitment will be ..
  51. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
  52. RNA APTAMER PROBES OF TRANSCRIPTIONAL MECHANISMS IN VIVO
    JOHN LIS; Fiscal Year: 2007
    ..The regulated in vivo expression of RNA aptamers that target disease-inducing proteins would prove to be of substantial therapeutic value. ..
  53. Applying logic to derive pathways from genome-wide data
    Einat Sprinzak; Fiscal Year: 2007
    ..Furthermore, I will apply the logic analysis to cancer datasets to illuminate the processes which lead cells to neoplastic transformation (Aim4). [unreadable] [unreadable]..
  54. The Role of MicroRNA in Hepatocarcinogenesis
    Kalpana Ghoshal; Fiscal Year: 2007
    ..Further, this proposal fits well with the mission/goals set forth by multiple institutes that include NCI, NIDDK and NIAAA n the etiology, prevention and treatment of hepatocellular carcinomas. [unreadable] [unreadable] [unreadable]..
  55. VIRAL REGULATION OF RIBOSOMAL RNA TRANSCRIPTION
    Lucio Comai; Fiscal Year: 2007
    ..we have developed biochemical assays aimed at dissecting the molecular events that lead to the activation of RNA polymerase I transcription upon viral infection...
  56. AMASTIN/TUZIN GENE CLUSTER OF TRYPANOSOMA CRUZI
    JOHN DONELSON; Fiscal Year: 2001
    ..Finally, we will assess whether amastin and/or tuzin can serve a target antigens for an improved diagnostic test for T. cruzi infection. ..