dna polymerase ii

Summary

Summary: A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms. It may be present in higher organisms and has an intrinsic molecular activity only 5% of that of DNA Polymerase I. This polymerase has 3'-5' exonuclease activity, is effective only on duplex DNA with gaps or single-strand ends of less than 100 nucleotides as template, and is inhibited by sulfhydryl reagents. EC 2.7.7.7.

Top Publications

  1. pmc Polymerase dynamics at the eukaryotic DNA replication fork
    Peter M J Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:4041-5. 2009
  2. pmc Division of labor at the eukaryotic replication fork
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Mol Cell 30:137-44. 2008
  3. pmc Studies on human DNA polymerase epsilon and GINS complex and their role in DNA replication
    Vladimir P Bermudez
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 286:28963-77. 2011
  4. pmc Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint
    Huiqiang Lou
    Braun Laboratories, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 32:106-17. 2008
  5. pmc Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity
    A Morrison
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Proc Natl Acad Sci U S A 88:9473-7. 1991
  6. ncbi Break-induced replication and telomerase-independent telomere maintenance require Pol32
    John R Lydeard
    MS029 Rosenstiel Centre, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nature 448:820-3. 2007
  7. pmc Yeast DNA polymerase epsilon participates in leading-strand DNA replication
    Zachary F Pursell
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Science 317:127-30. 2007
  8. pmc Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase ε
    Alan B Clark
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, United States
    DNA Repair (Amst) 10:476-82. 2011
  9. pmc CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast
    Sachiko Muramatsu
    National Institute of Genetics, Mishima, Japan
    Genes Dev 24:602-12. 2010
  10. pmc DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice
    Tina M Albertson
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Proc Natl Acad Sci U S A 106:17101-4. 2009

Research Grants

  1. DNA SEQUENCING OF UV INDUCED CHROMOSOMAL MUTATIONS
    Christopher Lawrence; Fiscal Year: 2003
  2. ENHANCING THE INTRACELLULAR FUNCTIONING OF HIV RIBOZYMES
    John Rossi; Fiscal Year: 2001
  3. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
  4. CSHL Symposium on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2006
  5. EPIGENETIC ACTIVATION OF THE HUMAN GROWTH HORMONE GENE
    Yugong Ho; Fiscal Year: 2007
  6. Molecular Epidemiology of Pancreatic Cancer
    Donghui Li; Fiscal Year: 2008
  7. RED CELL CYTOSKELETAL PROTEIN AND MITOSIS
    Shu Ching Huang; Fiscal Year: 2008
  8. Regulation of DNA Replication and Repair
    Mark Sutton; Fiscal Year: 2008
  9. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010
  10. COLD SPRING HARBOR SYMPOSIUM ON QUANTITATIVE BIOLOGY
    Bruce Stillman; Fiscal Year: 2005

Detail Information

Publications215 found, 100 shown here

  1. pmc Polymerase dynamics at the eukaryotic DNA replication fork
    Peter M J Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:4041-5. 2009
    ..In the more common short flap pathway, Pol delta coordinates with the flap endonuclease FEN1 to degrade initiator RNA, whereas in the long flap pathway, RNA removal is initiated by the Dna2 nuclease/helicase...
  2. pmc Division of labor at the eukaryotic replication fork
    Stephanie A Nick McElhinny
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Mol Cell 30:137-44. 2008
    ....
  3. pmc Studies on human DNA polymerase epsilon and GINS complex and their role in DNA replication
    Vladimir P Bermudez
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 286:28963-77. 2011
    ..We suggest that reduction in the level of Pol δ may be less deleterious because of its collision-and-release role in lagging strand synthesis...
  4. pmc Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint
    Huiqiang Lou
    Braun Laboratories, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 32:106-17. 2008
    ..We propose that Mrc1, which also interacts with the MCMs, may modulate coupling of polymerization and unwinding at the replication fork...
  5. pmc Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity
    A Morrison
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Proc Natl Acad Sci U S A 88:9473-7. 1991
    We have identified an amino-proximal sequence motif, Phe-Asp-Ile-Glu-Thr, in Saccharomyces cerevisiae DNA polymerase II that is almost identical to a sequence comprising part of the 3'----5' exonuclease active site of Escherichia coli ..
  6. ncbi Break-induced replication and telomerase-independent telomere maintenance require Pol32
    John R Lydeard
    MS029 Rosenstiel Centre, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nature 448:820-3. 2007
    ..We also note that Pol32 homologues have been identified both in fission yeast and in metazoans where telomerase-independent survivors with alternative telomere maintenance have also been identified...
  7. pmc Yeast DNA polymerase epsilon participates in leading-strand DNA replication
    Zachary F Pursell
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Science 317:127-30. 2007
    ....
  8. pmc Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase ε
    Alan B Clark
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, United States
    DNA Repair (Amst) 10:476-82. 2011
    ..g., during the repair of nicks resulting from rNMPs in DNA. The results make interesting predictions that can be tested...
  9. pmc CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast
    Sachiko Muramatsu
    National Institute of Genetics, Mishima, Japan
    Genes Dev 24:602-12. 2010
    ..We propose that CDK regulates the initiation of DNA replication in budding yeast through formation of the pre-LC...
  10. pmc DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice
    Tina M Albertson
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Proc Natl Acad Sci U S A 106:17101-4. 2009
    ..These findings distinguish Pol epsilon and delta functions in vivo and reveal tissue-specific requirements for DNA replication fidelity...
  11. ncbi Replication arrest-stimulated recombination: Dependence on the RecA paralog, RadA/Sms and translesion polymerase, DinB
    Susan T Lovett
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
    DNA Repair (Amst) 5:1421-7. 2006
    ..The role of DinB in bacteria may be analogous to translesion DNA polymerase eta in eukaryotes, recently shown to aid recombination...
  12. pmc The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress
    Mireille Tittel-Elmer
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada
    EMBO J 28:1142-56. 2009
    ....
  13. pmc In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta
    Y I Pavlov
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Genetics 159:47-64. 2001
    ..This is supported by the observation that the pol3-Y708A mutation is recessive and its mutator effect is partially suppressed by disruption of the REV3 gene...
  14. pmc Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint
    H Araki
    Research Institute for Microbial Diseases, Osaka University, Japan
    Proc Natl Acad Sci U S A 92:11791-5. 1995
    DPB11, a gene that suppresses mutations in two essential subunits of Saccharomyces cerevisiae DNA polymerase II(epsilon) encoded by POL2 and DPB2, was isolated on a multicopy plasmid...
  15. pmc Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer
    Rintaro Yoshida
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Eur J Hum Genet 19:320-5. 2011
    ..Our observations may suggest previously unrecognised complexities in the molecular abnormalities underlying the mutator phenotype in human neoplasms...
  16. pmc The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA
    Olga Chilkova
    Department of Medical Biochemistry and Biophysics, Department of Biochemistry, Umea University, 901 87 Umea, Sweden
    Nucleic Acids Res 35:6588-97. 2007
    ..We conclude that Pol epsilon and Pol delta exhibit comparable processivity, but are loaded on the primer-end via different mechanisms...
  17. pmc Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis
    Young Hoon Kang
    Program of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:6042-7. 2012
    ..These findings suggest that the coordinated action of these replication complexes supports leading strand synthesis...
  18. pmc Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex
    Henri Marc Bourbon
    Centre de Biologie du Developpement, UMR5547 CNRS Toulouse III, IFR109, Universite Paul Sabatier, 31062 Toulouse, France
    Nucleic Acids Res 36:3993-4008. 2008
    ..The implications of this comprehensive work for MED structure-function relationships are discussed...
  19. ncbi A versatile tool for conditional gene expression and knockdown
    Jolanta Szulc
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    Nat Methods 3:109-16. 2006
    ..These results open up promising perspectives for basic or translational research and for the development of gene-based therapeutics...
  20. ncbi Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases
    A M Holmes
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 96:415-24. 1999
    ..Replication is terminated by capture of the second end of the DSB...
  21. ncbi Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication
    Hiroyuki Araki
    Department of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, Japan
    Curr Opin Cell Biol 22:766-71. 2010
    ..Sld3, on the other hand, must be turned over to reassociate with origins. Pol ɛ functions as a component of the pre-LC as well as a replicative DNA polymerase at replication forks...
  22. pmc ATX1-generated H3K4me3 is required for efficient elongation of transcription, not initiation, at ATX1-regulated genes
    Yong Ding
    School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
    PLoS Genet 8:e1003111. 2012
    ..The roles of H3K4me3, ATX1, and AtCOMPASS-like may be of a general relevance for transcription of Trithorax-activated eukaryotic genes...
  23. pmc The high fidelity and unique error signature of human DNA polymerase epsilon
    Dagmara A Korona
    Department of Biochemistry and Tulane Cancer Center, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA 70112, USA
    Nucleic Acids Res 39:1763-73. 2011
    ..The base pair substitution specificity and high fidelity for frameshift errors observed for human Pol ε are distinct from the errors made by human Pol δ...
  24. pmc The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts
    Koh Shikata
    Laboratories for Biomolecular Networks, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    BMC Biochem 7:21. 2006
    ..Although the roles of replicative Pol alpha and Pol delta in chromosomal DNA replication are relatively well understood and well documented, the precise role of Pol epsilon in chromosomal DNA replication is not well understood...
  25. pmc Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas
    Claire Palles
    Molecular and Population Genetics Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Nat Genet 45:136-44. 2013
    ..Further analysis of published data showed that the recently described group of hypermutant, microsatellite-stable CRCs is likely to be caused by somatic POLE mutations affecting the exonuclease domain...
  26. ncbi Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells
    Tomoo Ogi
    Department of Molecular Medicine, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences, Nagasaki University, 1 12 4, Sakamoto, Nagasaki 852 8523, Japan
    Mol Cell 37:714-27. 2010
    ..The remaining repair synthesis is dependent on pol epsilon, recruitment of which is dependent on the alternative clamp loader CTF18-RFC...
  27. pmc DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae
    H Wang
    Howard Hughes Medical Institute, Verna and Marrs McLean Department of Biochemistry, Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 96:3824-9. 1999
    ..DRC1 and DPB11 show synthetic lethality and reciprocal dosage suppression. The Drc1 and Dpb11 proteins physically associate and function together to coordinate DNA replication and the cell cycle...
  28. pmc Replication-independent core histone dynamics at transcriptionally active loci in vivo
    Christophe Thiriet
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, USA
    Genes Dev 19:677-82. 2005
    ....
  29. ncbi Base analog 6-N-hydroxylaminopurine mutagenesis in the yeast Saccharomyces cerevisiae is controlled by replicative DNA polymerases
    P V Shcherbakova
    Department of Genetics, Sankt Petersburg University, Russia
    Mutat Res 369:33-44. 1996
    ..From the data obtained, we conclude that both DNA polymerase epsilon and delta participate in mutation generation by HAP...
  30. pmc Proofreading of ribonucleotides inserted into DNA by yeast DNA polymerase ɛ
    Jessica S Williams
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 11:649-56. 2012
    ..Collectively, the results indicate that although proofreading of an 'incorrect' sugar is less efficient than is proofreading of an incorrect base, Pol ɛ does proofread newly inserted rNMPs to enhance genome stability...
  31. pmc Structural and mutational studies of a hyperthermophilic intein from DNA polymerase II of Pyrococcus abyssi
    Zhenming Du
    Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    J Biol Chem 286:38638-48. 2011
    ..NMR spin relaxation indicates that the Pab PolII intein is significantly more rigid than mesophilic inteins, which may contribute to the higher optimal temperature for protein splicing...
  32. pmc Hypermutability of homonucleotide runs in mismatch repair and DNA polymerase proofreading yeast mutants
    H T Tran
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 17:2859-65. 1997
    ..Therefore, coding sequences containing long homonucleotide runs are likely to be at risk for mutational inactivation in cells lacking mismatch repair capability...
  33. ncbi The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae
    Tomoko Ohya
    Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:28099-108. 2002
    ..These results indicate that the DNA polymerase domain of Pol2p is required for rapid, efficient, and highly accurate chromosomal DNA replication in yeast...
  34. ncbi Resolving a fidelity paradox: why Escherichia coli DNA polymerase II makes more base substitution errors in AT- compared with GC-rich DNA
    Zhijie Wang
    Department of Biological Sciences, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, California 90089 1340, USA
    J Biol Chem 277:4446-54. 2002
    ..Unexpectedly, however, the opposite result was found for Escherichia coli DNA polymerase II (pol II)...
  35. pmc Dividing the workload at a eukaryotic replication fork
    Thomas A Kunkel
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, 111 T W Alexander Drive, National Institute of Environmental Health Sciences, National Institute of Health, DHHS, Research Triangle Park, NC 27709, USA
    Trends Cell Biol 18:521-7. 2008
    ..We also review earlier studies in light of this model and then consider prospects for future investigations of possible variations on this simple division of labor...
  36. pmc DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast
    Tetsuya Handa
    Graduate School of Science, Osaka University, Osaka, Japan
    Mol Biol Cell 23:3240-53. 2012
    ..These results demonstrate that Pol ε plays essential roles in both the assembly and progression of CMG helicase...
  37. ncbi Defect of Dpb2p, a noncatalytic subunit of DNA polymerase ɛ, promotes error prone replication of undamaged chromosomal DNA in Saccharomyces cerevisiae
    Joanna Kraszewska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Mutat Res 737:34-42. 2012
    ..The obtained results suggest that the structural integrity of Pol ɛ HE is a crucial contributor to accurate chromosomal DNA replication and, when compromised, favors participation of error prone DNA Pol ζ in this process...
  38. pmc Balancing eukaryotic replication asymmetry with replication fidelity
    Thomas A Kunkel
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Curr Opin Chem Biol 15:620-6. 2011
    ....
  39. pmc Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ε
    Anna Aksenova
    Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden
    PLoS Genet 6:e1001209. 2010
    ....
  40. pmc Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases
    S W Wong
    Department of Pathology, Stanford Medical School, Stanford University, CA 94305
    EMBO J 7:37-47. 1988
    ..Three of these conserved regions appear to comprise the functional active site required for deoxynucleotide interaction. Two putative DNA interacting domains are also identified...
  41. ncbi In vivo reconstitution of Saccharomyces cerevisiae DNA polymerase epsilon in insect cells. Purification and characterization
    Rajiv Dua
    Braun Laboratories, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 277:7889-96. 2002
    ..Mutations of the conserved cysteines in the putative zinc finger domain reduced zinc binding, indicating that cysteine ligands are directly involved in binding zinc...
  42. pmc DNA polymerase II (polB) is involved in a new DNA repair pathway for DNA interstrand cross-links in Escherichia coli
    M Berardini
    Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    J Bacteriol 181:2878-82. 1999
    ..In fact, recombination was precluded because the cells do not receive lesion-free homologous DNA.) Herein, DNA polymerase II is shown to be in this new pathway, since the replication efficiency (RE) is higher in a polB+ ( ..
  43. ncbi Human DNA polymerase epsilon: enzymologic mechanism and gap-filling synthesis
    D J Mozzherin
    Department of Pharmacological Sciences, University Medical Center, State University of New York at Stony Brook 11794 8651, USA
    Biochemistry 35:3572-7. 1996
    ..This observation is consistent with a specific role for pol epsilon in gap-filling in vivo. Gap-filling is essential for both replication and repair...
  44. pmc Pathway correcting DNA replication errors in Saccharomyces cerevisiae
    A Morrison
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC
    EMBO J 12:1467-73. 1993
    ..We propose that the POL3 3'-->5' exonuclease and the PMS1 mismatch repair system act on a common pathway analogous to the dnaQ-->mutHLS pathway of DNA replication error correction in Escherichia coli...
  45. pmc Replication of ribonucleotide-containing DNA templates by yeast replicative polymerases
    Danielle L Watt
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 10:897-902. 2011
    ..Overall, ribonucleotide bypass efficiencies are comparable to, and usually exceed, those for the common oxidative stress-induced lesion 8-oxo-guanine...
  46. pmc A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli
    S Rangarajan
    Department of Biological Sciences, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089 1340, USA
    Proc Natl Acad Sci U S A 96:9224-9. 1999
    ..At the present time, the molecular mechanism underlying replication restart is not understood. DNA polymerase II (pol II), encoded by the dinA (polB) gene, is induced as part of the global SOS response to DNA damage...
  47. ncbi The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling
    Emmanuelle Bitoun
    Department of Physiology, Anatomy and Genetics, Medical Research Council Functional Genetics Unit, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Hum Mol Genet 16:92-106. 2007
    ..Since these activities likely extend to the entire ALF protein family, this study also significantly inputs our understanding of the molecular basis of FRAXE mental retardation syndrome in which FMR2 expression is silenced...
  48. ncbi The 3'-->5' exonucleases of both DNA polymerases delta and epsilon participate in correcting errors of DNA replication in Saccharomyces cerevisiae
    A Morrison
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Mol Gen Genet 242:289-96. 1994
    ....
  49. pmc The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae
    H T Tran
    Chromosome Stability Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 19:2000-7. 1999
    ..Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes...
  50. pmc A novel DNA polymerase family found in Archaea
    Y Ishino
    Department of Molecular Biology, Biomolecular Engineering Research Institute, Suita, Osaka, Japan
    J Bacteriol 180:2232-6. 1998
    ..Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least ..
  51. pmc Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity
    I J Fijalkowska
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 179:7435-45. 1997
    ..The modified complex forces extension of the mismatch largely at the exclusion of proofreading and polymerase dissociation pathways. SOS mutagenesis targeted at replication-blocking DNA lesions likely proceeds in the same manner...
  52. ncbi DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli
    Magdalena Banach-Orlowska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland
    Mol Microbiol 58:61-70. 2005
    ..Here, we have investigated the role of DNA polymerase II (Pol II) (polB gene product)...
  53. ncbi Evidence that errors made by DNA polymerase alpha are corrected by DNA polymerase delta
    Y I Pavlov
    Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    Curr Biol 16:202-7. 2006
    ..Given that eukaryotes encode specialized, proofreading-deficient polymerases with even lower fidelity than pol alpha, such intermolecular proofreading could be relevant to several DNA transactions that control genome stability...
  54. pmc DNA polymerase epsilon is required for coordinated and efficient chromosomal DNA replication in Xenopus egg extracts
    S Waga
    Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 98:4978-83. 2001
    ..These findings strongly suggest that Pol epsilon, along with Pol alpha and Pol delta, is necessary for coordinated chromosomal DNA replication in eukaryotic cells...
  55. pmc Schizosaccharomyces pombe cdc20+ encodes DNA polymerase epsilon and is required for chromosomal replication but not for the S phase checkpoint
    G D'URSO
    University of Miami School of Medicine, Department of Biochemistry and Molecular Biology, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 94:12491-6. 1997
    ..We propose that the checkpoint signal operating in S phase depends on assembly of the replication initiation complex, and that this signal is generated prior to the elongation stage of DNA synthesis...
  56. pmc Roles of replication fork-interacting and Chk1-activating domains from Claspin in a DNA replication checkpoint response
    Joon Lee
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Biol Cell 16:5269-82. 2005
    ..Another implication of this work is that stable binding of Claspin to chromatin may play a role in other functions besides the activation of Chk1...
  57. ncbi Subunit interactions within the Saccharomyces cerevisiae DNA polymerase epsilon (pol epsilon ) complex. Demonstration of a dimeric pol epsilon
    R Dua
    Braun Laboratories, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 275:28816-25. 2000
    ..This suggests, but does not prove, that dimerization may also occur in vivo and be essential for DNA replication...
  58. pmc Translesion synthesis of abasic sites by yeast DNA polymerase epsilon
    Nasim Sabouri
    Department of Medical Biochemistry and Biophysics, Umea University, SE 901 87 Umea, Sweden
    J Biol Chem 284:31555-63. 2009
    ..This characteristic may allow other pathways to rescue leading strand synthesis when stalled at an abasic site...
  59. pmc Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS
    Ryan L Frisch
    Department of Molecular and Human Genetics, Baylor College of Medicine, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    J Bacteriol 192:4694-700. 2010
    ..The results identify a second DNA polymerase contributing to stress-induced mutagenesis and show that RpoS promotes mutagenesis by more than the simple upregulation of dinB...
  60. pmc DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae
    H Araki
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Proc Natl Acad Sci U S A 88:4601-5. 1991
    The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced...
  61. ncbi The quaternary structure of DNA polymerase epsilon from Saccharomyces cerevisiae
    Olga Chilkova
    Department of Medical Biochemistry and Biophysics, Umea University, Sweden
    J Biol Chem 278:14082-6. 2003
    ..Thus, both DNA polymerase delta and Pol epsilon are purified as monomeric complexes, in agreement with accumulating evidence that Pol delta and Pol epsilon are located on opposite strands of the eukaryotic replication fork...
  62. pmc Competition of Escherichia coli DNA polymerases I, II and III with DNA Pol IV in stressed cells
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 5:e10862. 2010
    ..This mutagenesis assay reveals the DNA polymerases operating in DSB repair during stress and also provides a sensitive indicator for DNA polymerase competition and choice in vivo...
  63. pmc Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion
    Shaune Edwards
    Braun Laboratories, California Institute of Technology, Pasadena, California 91125, USA
    Mol Cell Biol 23:2733-48. 2003
    ..We also show that trf5 Delta mutants, like trf4 Delta mutants, are defective in DNA repair and sister chromatid cohesion...
  64. pmc Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae
    H Araki
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Nucleic Acids Res 19:4867-72. 1991
    b>DNA polymerase II purified from Saccharomyces cerevisiae contains polypeptides with apparent molecular masses of greater than 200, 80, 34, 30 and 29 kDa, the two largest of which (subunits A and B) are encoded by the essential genes POL2 ..
  65. pmc DNA polymerase epsilon: a polymerase of unusual size (and complexity)
    Zachary F Pursell
    Department of Health and Human Services, Laboratory of Molecular Genetics, National Institute of Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Prog Nucleic Acid Res Mol Biol 82:101-45. 2008
  66. pmc Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis
    W Liu
    Division of Biochemistry and Molecular Biology, 229 Stanley Hall, University of California, Berkeley, CA 94720 3206, USA
    Nucleic Acids Res 28:4180-8. 2000
    ..p140 is unlikely to be able to functionally replace p261 in vivo, since it does not bind to PCNA or the other pol epsilon subunits...
  67. ncbi Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae
    Kikuo Shimizu
    The Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:37422-9. 2002
    ....
  68. pmc MCM proteins are associated with RNA polymerase II holoenzyme
    K Yankulov
    Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Mol Cell Biol 19:6154-63. 1999
    ..These results suggest a new function for MCM proteins as components of the Pol II transcriptional apparatus...
  69. pmc Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae
    T Ohya
    Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nucleic Acids Res 28:3846-52. 2000
    ..cerevisiae, even if it is not essential for cell growth. Structural homologues of DPB4 are present in other eukaryotic genomes, suggesting that the complex structure of S. cerevisiae Polepsilon is conserved in eukaryotes...
  70. ncbi Saccharomyces cerevisiae DNA polymerase delta: high fidelity for base substitutions but lower fidelity for single- and multi-base deletions
    John M Fortune
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 280:29980-7. 2005
    ..Thus, strand slippage during replication by wild type Pol delta may be a primary source of insertion and deletion mutagenesis in eukaryotic genomes...
  71. pmc Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast
    H Masumoto
    Division of Microbial Genetics, National Institute of Genetics, Shizuoka, Japan
    Mol Cell Biol 20:2809-17. 2000
    ..This indicates that Dpb11 may also be involved in the regulation of late-origin firing. Overall, these results suggest that Dpb11 controls the association between DNA polymerases alpha and epsilon and the ARS...
  72. pmc Structural relationship between DNA polymerases epsilon and epsilon* and their occurrence in eukaryotic cells
    L Uitto
    Biocenter Oulu, University of Oulu, Finland
    Nucleic Acids Res 23:244-7. 1995
    ....
  73. ncbi RAD9 and DNA polymerase epsilon form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae
    T A Navas
    Verna and Mars McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 10:2632-43. 1996
    ..Each of these pathways subsequently activates the central transducers Mec1p/Esr1p/Sad3p and Rad53p/Mec2p/Sad1p, which are required for both cell-cycle arrest and transcriptional responses...
  74. ncbi DNA repair synthesis in human fibroblasts requires DNA polymerase delta
    C Nishida
    Department of Biochemistry, University of California, Berkeley 94720
    J Biol Chem 263:501-10. 1988
    ....
  75. ncbi Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy
    Francisco J Asturias
    Department of Structural Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 13:35-43. 2006
    ..Together, these observations suggest a mechanism for interaction of Pol epsilon with DNA that might explain how the structure of the enzyme contributes to its intrinsic processivity...
  76. ncbi Further characterization of HeLa DNA polymerase epsilon
    G Chui
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202, USA
    J Biol Chem 270:7799-808. 1995
    ....
  77. ncbi Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase epsilon
    T Kesti
    Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 268:10238-45. 1993
    ..that human DNA polymerase epsilon catalytic polypeptide is a homolog of the yeast Saccharomyces cerevisiae DNA polymerase II catalytic polypeptide. The proteins are 39% identical...
  78. ncbi Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon
    Y Li
    Division of Biochemistry and Molecular Biology, Barker Hall, University of California, Berkeley, California 94720 3202, USA
    J Biol Chem 272:32337-44. 1997
    ..The 59,434-Da predicated polypeptide has 26% identity and 44% homology to the yeast pol epsilon 80-kDa subunit, DPB2. Using fluorescence in situ hybridization, the human pol epsilon p59 locus (DPE2) was assigned to chromosome 14q13-q21...
  79. ncbi Distinctive activities of DNA polymerases during human DNA replication
    Anna K Rytkönen
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland
    FEBS J 273:2984-3001. 2006
    ..These data are consistent with models where pols delta and epsilon pursue their functions at least partly independently during DNA replication...
  80. pmc Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae
    Malgorzata Jaszczur
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
    Genetics 178:633-47. 2008
    ..Our results show that DNA polymerase subunits other than those housing the DNA polymerase and 3' --> 5' exonuclease are essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells...
  81. pmc Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations
    Youri I Pavlov
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institute of Health, DHHS, Research Triangle Park, NC 27709, USA
    BMC Biol 2:11. 2004
    ..Here we investigate the mechanism responsible for the mutator effect...
  82. ncbi Human DNA polymerase epsilon colocalizes with proliferating cell nuclear antigen and DNA replication late, but not early, in S phase
    Jill Fuss
    Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720 3206, USA
    J Biol Chem 277:8658-66. 2002
    ..It is hypothesized from these observations that pol epsilon and PCNA have separate but associated functions early in S phase and that pol epsilon participates with PCNA in DNA replication late in S phase...
  83. pmc Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae
    Malgorzata Jaszczur
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Mutat Res 669:27-35. 2009
    ..We propose that structural integrity of the Pol epsilon holoenzyme is essential for genetic stability in S. cerevisiae cells...
  84. pmc Conformational changes associated with template commitment in ATP-dependent chromatin remodeling by ISW2
    Vamsi K Gangaraju
    Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901 4413, USA
    Mol Cell 35:58-69. 2009
    ..These additional contacts are independent of the ATPase domain tracking along nucleosomal DNA and are maintained as ISW2 moves nucleosomes on DNA...
  85. pmc Schizosaccharomyces pombe cells lacking the amino-terminal catalytic domains of DNA polymerase epsilon are viable but require the DNA damage checkpoint control
    W Feng
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101 6129, USA
    Mol Cell Biol 21:4495-504. 2001
    ..This unique and essential function of the C terminus is preserved in the absence of the N-terminal catalytic domains, suggesting that the C terminus can interact with and recruit other DNA polymerases to the site of initiation...
  86. pmc Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant
    Damian Gawel
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Bacteriol 190:1730-42. 2008
    ..Overall, the results provide insight into the interplay of the various DNA polymerases, and of tau subunit, in securing a high fidelity of replication...
  87. ncbi Elevated expression of DNA polymerase II increases spontaneous mutagenesis in Escherichia coli
    Abu Amar M Al Mamun
    University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Department of Microbiology and Molecular Genetics, International Center for Public Health, 225 Warren Street, Newark, NJ 07101 1709, United States
    Mutat Res 625:29-39. 2007
    Escherichia coli DNA polymerase II (Pol-II), encoded by the SOS-regulated polB gene, belongs to the highly conserved group B (alpha-like) family of "high-fidelity" DNA polymerases...
  88. pmc Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase epsilon
    Zachary F Pursell
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Nucleic Acids Res 35:3076-86. 2007
    ....
  89. ncbi Distinct roles of DNA polymerases delta and epsilon at the replication fork in Xenopus egg extracts
    Tomoyuki Fukui
    Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Genes Cells 9:179-91. 2004
    ..Therefore, our data strongly suggest the possibilities that Poldelta is essential for lagging strand synthesis and that this function of Poldelta cannot be substituted for by Polepsilon...
  90. ncbi Cell cycle-dependent phosphorylation of the DNA polymerase epsilon subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase
    Tapio Kesti
    Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:14245-55. 2004
    ..We suggest that phosphorylation of Dpb2 during late G(1) phase at CDK consensus sites facilitates the interaction with Pol2 or the activity of Polepsilon..
  91. ncbi DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae
    Shin Ichiro Hiraga
    Laboratories for Biomolecular Networks, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Genes Cells 10:297-309. 2005
    ..These data collectively suggest that bidirectional replication occurs at specific foci in yeast chromosomes and that pol alpha, -delta, and -epsilon localize and function together at multiple replication forks during S phase...
  92. ncbi Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly
    Toshiaki Tsubota
    Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Genes Cells 8:873-88. 2003
    ..In order to clarify molecular mechanisms employed in the multiple tasks of Pol epsilon, we have been characterizing the interaction between Pol epsilon and DNA...
  93. pmc Schizosacchromyces pombe Dpb2 binds to origin DNA early in S phase and is required for chromosomal DNA replication
    Wenyi Feng
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101 6129, USA
    Mol Biol Cell 14:3427-36. 2003
    ....
  94. ncbi Unique error signature of the four-subunit yeast DNA polymerase epsilon
    Polina V Shcherbakova
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, North Carolina 27709, USA
    J Biol Chem 278:43770-80. 2003
    ..We discuss the implications of these findings for the role of Pol epsilon polymerase activity in DNA replication...
  95. ncbi DNA polymerase epsilon - more than a polymerase
    Helmut Pospiech
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland
    ScientificWorldJournal 3:87-104. 2003
    ..In addition to its role in DNA replication, DNA polymerase epsilon fulfils roles in the DNA synthesis step of nucleotide excision and base excision repair, and has been implicated in recombinational processes in the cell...
  96. ncbi GINS is a DNA polymerase epsilon accessory factor during chromosomal DNA replication in budding yeast
    Takashi Seki
    Laboratories for Biomolecular Networks, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    J Biol Chem 281:21422-32. 2006
    ..Based on these results, we propose a model for molecular dynamics at eukaryotic chromosomal replication fork...
  97. ncbi Double-stranded DNA binding, an unusual property of DNA polymerase epsilon, promotes epigenetic silencing in Saccharomyces cerevisiae
    Toshiaki Tsubota
    Department of Molecular Biology, Graduate School of Biological Sciences and Graduate School of Information Science, Nara Institute of Science and Technology, Ikoma, Nara 630 0192, Japan
    J Biol Chem 281:32898-908. 2006
    ..We conclude that the dsDNA-binding property of Pol epsilon is required for epigenetic silencing at telomeres...
  98. pmc Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae
    Tetsushi Iida
    Division of Microbial Genetics, National Institute of Genetics, SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 24:217-27. 2004
    ..We therefore propose that inheritance of specific epigenetic states of a telomere requires at least two counteracting regulators...
  99. ncbi Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme
    R K Hamatake
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 265:4072-83. 1990
    We have purified yeast DNA polymerase II to near homogeneity as a 145-kDa polypeptide. During the course of this purification we have detected and purified a novel form of DNA polymerase II that we designate as DNA polymerase II...
  100. pmc A role for the replication proteins PCNA, RF-C, polymerase epsilon and Cdc45 in transcriptional silencing in Saccharomyces cerevisiae
    A E Ehrenhofer-Murray
    Department of Molecular and Cell Biology, Division of Genetics, University of California, Berkeley, California 94720, USA
    Genetics 153:1171-82. 1999
    ..Restoration of silencing by PCNA and CDC45 mutations required the origin recognition complex binding site of the HMR-E silencer. Several models for the precise role of these replication proteins in silencing are discussed...
  101. pmc Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli
    P L Foster
    Department of Environmental Health, Boston University School of Public Health, MA 02118 2394, USA
    Proc Natl Acad Sci U S A 92:7951-5. 1995
    ..Mutations produced by both Pol III and Pol II ex1 were corrected by the mutHLS mismatch repair system...

Research Grants24

  1. DNA SEQUENCING OF UV INDUCED CHROMOSOMAL MUTATIONS
    Christopher Lawrence; Fiscal Year: 2003
    ..of the gene products that are responsible for efficient replication past a T-T dimer in cells lacking DNA polymerase II, IV, and V and proofreading activity; identification of the MucB residues responsible for its differences in ..
  2. ENHANCING THE INTRACELLULAR FUNCTIONING OF HIV RIBOZYMES
    John Rossi; Fiscal Year: 2001
    ..The results from this program will greatly facilitate the effective use of ribozyme treatment for HIV-1 infection in a gene therapy setting. ..
  3. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
    ..We will extend genetic analysis of radC to determine what role it may play in repair and recombination. ..
  4. CSHL Symposium on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2006
    ..The meeting will address the phenomenon of small ribonucleic acids and their function in a variety of regulatory phenomena in the cell. [unreadable] [unreadable] [unreadable]..
  5. EPIGENETIC ACTIVATION OF THE HUMAN GROWTH HORMONE GENE
    Yugong Ho; Fiscal Year: 2007
    ..Such understanding establishes a basis for advances in diagnosis and treatment of endocrine disorders. ..
  6. Molecular Epidemiology of Pancreatic Cancer
    Donghui Li; Fiscal Year: 2008
    ..Such information will be valuable for the primary prevention of this deadly disease. ..
  7. RED CELL CYTOSKELETAL PROTEIN AND MITOSIS
    Shu Ching Huang; Fiscal Year: 2008
    ..Finally, we shall define the relationship between changes in the expression of these 4.1R isoforms and the transition from proliferation to post-mitotic states during erythroid differentiation. ..
  8. Regulation of DNA Replication and Repair
    Mark Sutton; Fiscal Year: 2008
    ..abstract_text> ..
  9. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010
    ..LAY DESCRIPTION: We will study a protein present in all bacteria that appears to control their growth. This will provide important missing information about how bacteria divide and may provide a target for antibiotics. ..
  10. COLD SPRING HARBOR SYMPOSIUM ON QUANTITATIVE BIOLOGY
    Bruce Stillman; Fiscal Year: 2005
    ..The annual Symposia will continue to be planned to further the progress of advancements in biomedical science. ..
  11. CSHL Symposium on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2004
    ..The meeting will address the molecular basis of epigenetic phenomena and their relationship to chromatin and chromosome structure and function. ..
  12. EUKARYOTIC DNA REPLICATION CONFERENCE
    Bruce Stillman; Fiscal Year: 2005
    ..The subsequent meetings (2003 and 2005) will follow a similar format which will include topics that are highly relevant to the current research at the time of the meeting. ..
  13. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2002
    ..Improper regulation of these processes in humans may lead to cancer. ..
  14. Gordon Research Conference on Mutagenesis, 2002
    MYRON GOODMAN; Fiscal Year: 2002
    ..abstract_text> ..
  15. BIOCHEMICAL BASIS OF SOS--INDUCED MUTAGENESIS
    MYRON GOODMAN; Fiscal Year: 2002
    ..E. coli DNA polymerase II, is also induced in response to DNA damage, as part of the SOS regulon, but does not appear to take part in ..
  16. Conference--CSHL on Quantitative Biology
    Bruce Stillman; Fiscal Year: 2003
    ..The annual Symposia will continue to be planned to further the progress of advancements in biomedical science. This proposal requests support for 2003 to cover the 68th Cold Spring Harbor Symposium. ..
  17. Mechanism of Gamma-Globin Gene Activation in the Adult
    Qiliang Li; Fiscal Year: 2004
    ..Such a development will have important consequences for the treatment of patients with sickle cell disease or beta thalassemia syndromes. ..
  18. GENETIC ANALYSIS OF BASAL TRANSCRIPTION IN YEAST
    Gregory Prelich; Fiscal Year: 2009
    ..Mutations in the genes that encode homologous proteins are associated with human diseases. ..
  19. Analysis of the COP9 signalosome for Retinoblastoma function
    David N Arnosti; Fiscal Year: 2010
    ....
  20. Epigenetic Regulation of Germ Cell Differentiation from a Stem Cell Lineage
    Xin Chen; Fiscal Year: 2008
    ..cellular differentiation during male germ cell development. I am committed to the research in biomedical science, and to the education of future scientists. ..
  21. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  22. STRUCTURAL STUDY OF EUKARYOTIC TRANSCRIPTION REGULATION
    Francisco Asturias; Fiscal Year: 2004
    ..By identifying structural differences and similarities between the complexes we hope to contribute to the understanding of what increasingly appears as a universal mechanism for transcription control. ..
  23. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  24. Effects of Formamidopyrimidine Lesions on DNA
    Marc Greenberg; Fiscal Year: 2006
    ..abstract_text> ..