methyltransferases

Summary

Summary: A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.

Top Publications

  1. pmc 2'-O methylation of the viral mRNA cap evades host restriction by IFIT family members
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 468:452-6. 2010
  2. pmc MLL-rearranged leukemia is dependent on aberrant H3K79 methylation by DOT1L
    Kathrin M Bernt
    Division of Hematology Oncology, Children s Hospital, Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 20:66-78. 2011
  3. ncbi MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome
    Yolanda Camara
    Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
    Cell Metab 13:527-39. 2011
  4. ncbi SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase fold
    Jennifer L Martin
    Centre for Drug Design and Development, and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Curr Opin Struct Biol 12:783-93. 2002
  5. ncbi Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation
    Xiaofeng Cao
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 917 Datun Road, Beijing 100101, China
    Curr Biol 13:2212-7. 2003
  6. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
  7. pmc Polymorphisms in arsenic(+III oxidation state) methyltransferase (AS3MT) predict gene expression of AS3MT as well as arsenic metabolism
    Karin Engström
    Department of Laboratory Medicine, Section of Occupational and Environmental Medicine, Lund University, Lund, Sweden
    Environ Health Perspect 119:182-8. 2011
  8. ncbi Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
  9. ncbi Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing
    Xiaofeng Cao
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles 90095, USA
    Curr Biol 12:1138-44. 2002
  10. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001

Detail Information

Publications367 found, 100 shown here

  1. pmc 2'-O methylation of the viral mRNA cap evades host restriction by IFIT family members
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 468:452-6. 2010
    ..are methylated at the N-7 and 2'-O positions of the 5' guanosine cap by specific nuclear and cytoplasmic methyltransferases (MTases), respectively...
  2. pmc MLL-rearranged leukemia is dependent on aberrant H3K79 methylation by DOT1L
    Kathrin M Bernt
    Division of Hematology Oncology, Children s Hospital, Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 20:66-78. 2011
    ..Suppression of MLL translocation-associated gene expression corresponded with dependence of MLL-AF9 leukemia on Dot1l in vivo. These data point to DOT1L as a potential therapeutic target in MLL-rearranged leukemia...
  3. ncbi MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome
    Yolanda Camara
    Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
    Cell Metab 13:527-39. 2011
    ..Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation. Our results thus show that MTERF4 is an important regulator of translation in mammalian mitochondria...
  4. ncbi SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase fold
    Jennifer L Martin
    Centre for Drug Design and Development, and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Curr Opin Struct Biol 12:783-93. 2002
    ..Over the past two years, there has been a significant increase in the number of structures that are known to incorporate this fold, including several uncharacterized proteins and two proteins that lack methyltransferase activity...
  5. ncbi Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation
    Xiaofeng Cao
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 917 Datun Road, Beijing 100101, China
    Curr Biol 13:2212-7. 2003
    ..These DNA methyltransferases appear to act downstream of siRNAs, since drm1 drm2 cmt3 triple mutants show a lack of non-CpG methylation ..
  6. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products...
  7. pmc Polymorphisms in arsenic(+III oxidation state) methyltransferase (AS3MT) predict gene expression of AS3MT as well as arsenic metabolism
    Karin Engström
    Department of Laboratory Medicine, Section of Occupational and Environmental Medicine, Lund University, Lund, Sweden
    Environ Health Perspect 119:182-8. 2011
    ..The fraction of urinary MMA is a marker for susceptibility to As-related diseases...
  8. ncbi Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
    ..Furthermore, we found that K79 methylation level is regulated throughout the cell cycle. Thus, our studies reveal a new methylation site and define a novel family of histone lysine methyltransferase...
  9. ncbi Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing
    Xiaofeng Cao
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles 90095, USA
    Curr Biol 12:1138-44. 2002
    ..Arabidopsis has two types of methyltransferases with demonstrated maintenance activity: MET1, which maintains CpG methylation and is homologous to mammalian ..
  10. ncbi Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
    A J Bannister
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nature 410:120-4. 2001
    ..This model may also explain the stable inheritance of the heterochromatic state...
  11. pmc A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity
    Philippe Cloutier
    Institut de recherches cliniques de Montreal IRCM, Montreal, Quebec, Canada
    PLoS Genet 9:e1003210. 2013
    ..Recent genome-wide analyses have considerably extended the list of human genes encoding putative methyltransferases. Studies on protein methyltransferases have revealed that the regulatory function of methylation is not ..
  12. ncbi Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9
    Dorothea Greiner
    Adolf Butenandt Institute, Department of Molecular Biology, Histone Modifications Group, Ludwig Maximillians University of Munich, Schillerstr 44, 80336 Munich, Germany
    Nat Chem Biol 1:143-5. 2005
    ..Chaetocin is specific for the methyltransferase SU(VAR)3-9 both in vitro and in vivo and may therefore be used to study heterochromatin-mediated gene repression...
  13. pmc An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization
    Marie Pierre Egloff
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS et Université Aix Marseille I et II, ESIL, Campus de Luminy, F 13288 Marseille Cedex 09, France
    EMBO J 21:2757-68. 2002
    ..RNA polymerase NS5 of flaviviruses presents a characteristic motif of S-adenosyl-L-methionine-dependent methyltransferases at its N-terminus, and polymerase motifs at its C-terminus...
  14. ncbi hDOT1L links histone methylation to leukemogenesis
    Yuki Okada
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Cell 121:167-78. 2005
    ..Our studies thus establish that mistargeting of hDOT1L to Hoxa9 plays an important role in MLL-AF10-mediated leukemogenesis and suggests that the enzymatic activity of hDOT1L may provide a potential target for therapeutic intervention...
  15. ncbi Establishment and maintenance of a heterochromatin domain
    Ira M Hall
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 297:2232-7. 2002
    ..This work defines sequential requirements for the initiation and propagation of regional heterochromatic domains...
  16. ncbi Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA
    Maria Falkenberg
    Department of Medical Nutrition, Karolinska Institutet, Novum, Huddinge Hospital, S 141 86 Huddinge, Sweden
    Nat Genet 31:289-94. 2002
    ..The presence of two proteins that interact with mammalian POLRMT may allow flexible regulation of mtDNA gene expression in response to the complex physiological demands of mammalian metabolism...
  17. pmc Jmjd2b antagonizes H3K9 trimethylation at pericentric heterochromatin in mammalian cells
    Barna D Fodor
    Research Institute of Molecular Pathology IMP, The Vienna Biocenter, A 1030 Vienna, Austria
    Genes Dev 20:1557-62. 2006
    ..These data reveal that certain members of the jmjC class of hydroxylases can work in a pathway that actively antagonizes a histone lysine trimethyl state...
  18. pmc The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA
    Britta Meyer
    Cluster of Excellence Frankfurt Macromolecular Complexes, Max von Laue Str 9, D 60438 Frankfurt M, Germany
    Nucleic Acids Res 39:1526-37. 2011
    ..Furthermore, the BCS mutation prevents nucleolar accumulation of Nep1, which could be the reason for reduced growth in yeast and the Bowen-Conradi syndrome...
  19. pmc Ribose 2'-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5
    Roland Zust
    Institute of Immunobiology, Kantonal Hospital St Gallen, St Gallen, Switzerland
    Nat Immunol 12:137-43. 2011
    ..Likewise, many viruses that replicate in the cytoplasm of eukaryotes have evolved 2'-O-methyltransferases to autonomously modify their mRNAs...
  20. pmc Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase
    Kavitha Marapakala
    Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, Florida 33199, United States
    Biochemistry 51:944-51. 2012
    ..The rate-limiting step was identified as the conversion of DMAs(III) to trimethylarsine, and DMAs(III) accumulates as the principal product...
  21. pmc The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate
    Sandra Blanco
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS Genet 7:e1002403. 2011
    ..Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate...
  22. ncbi A new metabolic pathway of arsenite: arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19
    Toru Hayakawa
    Environmental Health Sciences Division, National Institute for Environmental Studies, 16 2 Onogawa, Tsukuba, 305 8506, Ibaraki, Japan
    Arch Toxicol 79:183-91. 2005
    ..Metabolism of iAsIII to methylated arsenicals by Cyt19 was via ATG and MADG rather than by oxidative methylation of iAsIII and MMA(III)...
  23. pmc Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities
    Agnieszka Zylicz-Stachula
    Division of Theoretical Physical Chemistry, Department of Chemistry, University of Gdansk, Poland
    BMC Mol Biol 13:13. 2012
    ..Members include related thermophilic REases with five distinct specificities: TspGWI, TaqII, Tth111II/TthHB27I, TspDTI and TsoI...
  24. pmc Structural insights into methyltransferase KsgA function in 30S ribosomal subunit biogenesis
    Daniel Boehringer
    Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland
    J Biol Chem 287:10453-9. 2012
    ..We suggest that the activation of methyltransferase activity and subsequent dissociation of KsgA control conformational changes in helix 44 required for final rRNA processing and translation initiation...
  25. pmc DOT1L regulates dystrophin expression and is critical for cardiac function
    Anh T Nguyen
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, NC 27599, USA
    Genes Dev 25:263-74. 2011
    ..In addition, our study may open new avenues for the diagnosis and treatment of human heart disease...
  26. ncbi Regulation of chromatin structure by site-specific histone H3 methyltransferases
    S Rea
    Research Institute of Molecular Pathology, The Vienna Biocenter, Austria
    Nature 406:593-9. 2000
    ..homologues of Drosophila Su(var)3-9 and of Schizosaccharomyces pombe clr4--encode histone H3-specific methyltransferases that selectively methylate lysine 9 of the amino terminus of histone H3 in vitro...
  27. ncbi ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation
    Tatsuya Nakamura
    Kimmel Cancer Center and Department of Microbiology, Jefferson Medical College, Philadelphia, PA 19107, USA
    Mol Cell 10:1119-28. 2002
    ..In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter...
  28. pmc The Cfr rRNA methyltransferase confers resistance to Phenicols, Lincosamides, Oxazolidinones, Pleuromutilins, and Streptogramin A antibiotics
    Katherine S Long
    Institute of Molecular Biology and Physiology, University of Copenhagen, Sølvgade 83 H, DK 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 50:2500-5. 2006
    ..In addition, the findings described in this study represent the first report of a gene conferring transferable resistance to pleuromutilins and oxazolidinones...
  29. ncbi Stabilization of Suv39H1 by SirT1 is part of oxidative stress response and ensures genome protection
    Laia Bosch-Presegué
    Chromatin Biology Laboratory, Cancer Epigenetics and Biology Program, Institut d Investigacio Biomedica de Bellvitge, Avinguda Gran Via de l Hospitalet 199 203, 08907 L Hospitalet de Llobregat, Barcelona, Spain
    Mol Cell 42:210-23. 2011
    ..These observations reflect a direct link between oxidative stress response and Suv39h1 and support a dynamic view of heterochromatin, in which its structure adapts to cell physiology...
  30. pmc A subcomplex of human mitochondrial RNase P is a bifunctional methyltransferase--extensive moonlighting in mitochondrial tRNA biogenesis
    Elisa Vilardo
    Center for Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, Austria
    Nucleic Acids Res 40:11583-93. 2012
    ..In contrast to all the related methyltransferases, the human mitochondrial enzyme, moreover, requires a short-chain dehydrogenase as a partner protein...
  31. ncbi Exogenously acquired 16S rRNA methyltransferases found in aminoglycoside-resistant pathogenic Gram-negative bacteria: an update
    Jun ichi Wachino
    Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan
    Drug Resist Updat 15:133-48. 2012
    ..The worldwide dissemination of 16S-RMTases is becoming a serious global concern and this implies the necessity to continue investigations on the trend of 16S-RMTases to restrict their further worldwide dissemination...
  32. ncbi Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
    J Nakayama
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 292:110-3. 2001
    ..These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly...
  33. pmc Epigenetic histone H3 lysine 9 methylation in metabolic memory and inflammatory phenotype of vascular smooth muscle cells in diabetes
    Louisa M Villeneuve
    Graduate School of Biological Sciences and Department of Diabetes, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 105:9047-52. 2008
    ..Dysregulation of epigenetic histone modifications may be a major underlying mechanism for metabolic memory and sustained proinflammatory phenotype of diabetic cells...
  34. pmc Structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation
    A Abdul Ajees
    Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, 33199, United States
    Biochemistry 51:5476-85. 2012
    ..A homology model of human As(III) S-adenosylmethionine methyltransferase with the location of known polymorphisms was constructed. The structure provides insights into the mechanism of substrate binding and catalysis...
  35. pmc Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1
    Joanna Wysocka
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Genes Dev 17:896-911. 2003
    ..These results suggest that HCF-1 can broadly regulate transcription, both positively and negatively, through selective modulation of chromatin structure...
  36. pmc Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression
    Brian D Strahl
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 22:1298-306. 2002
    ..These results suggest that Set2 and methylation at H3 Lys36 play a role in the repression of gene transcription...
  37. pmc Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain
    Jessica M Davison
    Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 284:1982-9. 2009
    ..The mRNA and protein expression of histone methyltransferases G9a and Suv39h1 were directly related to the availability of choline...
  38. ncbi SIRT1 regulates the histone methyl-transferase SUV39H1 during heterochromatin formation
    Alejandro Vaquero
    Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Jersey 08854, USA
    Nature 450:440-4. 2007
    ..These findings demonstrate a functional link between the heterochromatin-related histone methyltransferase SUV39H1 and the histone deacetylase SIRT1...
  39. ncbi Unsafe SETs: histone lysine methyltransferases and cancer
    Robert Schneider
    Wellcome Cancer Research UK Institute and Department of Pathology, Tennis Court Road, Cambridge, UK CB2 1QR
    Trends Biochem Sci 27:396-402. 2002
    ..This article surveys the evidence for a connection between SET-domain-containing proteins and cancer. It proposes that deregulation of SET-domain function has an important role in carcinogenesis...
  40. ncbi A radically different mechanism for S-adenosylmethionine-dependent methyltransferases
    Tyler L Grove
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Science 332:604-7. 2011
    ..signaling, and epigenetic programming and is most often achieved by S-adenosylmethionine (SAM)-dependent methyltransferases. Most employ an S(N)2 mechanism to methylate nucleophilic sites on their substrates, but recently, radical ..
  41. pmc Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes
    Chhabi K Govind
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 39:234-46. 2010
    ..A strong correlation between increased acetylation and lower histone occupancy in HDA mutants implies that histone acetylation is important for nucleosome eviction...
  42. pmc Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae
    S D Briggs
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    Genes Dev 15:3286-95. 2001
    ..Taken together, these data suggest that Set1-mediated H3 Lys4 methylation is required for normal cell growth and transcriptional silencing...
  43. pmc Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells
    Daniela Meilinger
    Department of Biology II, Center for Integrated Protein Science Munich CIPSM, Ludwig Maximilians University Munich, Grosshaderner Street 2, 82152 Planegg Martinsried, Germany
    EMBO Rep 10:1259-64. 2009
    ..Here, we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a and Dnmt3b...
  44. pmc Hen1 is required for oocyte development and piRNA stability in zebrafish
    Leonie M Kamminga
    Hubrecht Institute KNAW, University Medical Centre Utrecht, Utrecht, The Netherlands
    EMBO J 29:3688-700. 2010
    ..This system discriminates between piRNA targets and is required for ovary development and fully efficient transposon silencing...
  45. ncbi Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 123:593-605. 2005
    ..This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2...
  46. pmc Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity
    Elizabeth H Bayne
    Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
    Cell 140:666-77. 2010
    ..We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification...
  47. pmc Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity
    Vicki McCulloch
    Department of Biochemistry, Rollins Research Center, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
    Mol Cell Biol 23:5816-24. 2003
    ..in collaboration with the high-mobility group box transcription factor, h-mtTFA, and are homologous to rRNA methyltransferases. In fact, the dual-function nature of h-mtTFB1 was recently demonstrated by its ability to methylate a ..
  48. pmc Heterochromatic genome stability requires regulators of histone H3 K9 methylation
    Jamy C Peng
    Lawrence Berkeley National Laboratory, Department of Genome and Computational Biology, Berkeley, California, USA
    PLoS Genet 5:e1000435. 2009
    ..Similar effects of lower magnitude were observed in animals that lack the RNA interference pathway component Dcr2. These results suggest that the H3K9 methylation and RNAi pathways ensure heterochromatin stability...
  49. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  50. ncbi Structure of the catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase
    Jinrong Min
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell 112:711-23. 2003
    ..Unlike other histone methyltransferases, Dot1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3...
  51. pmc An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf development
    Genji Qin
    National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    Plant Cell 17:2693-704. 2005
    ..The identification of IAMT1 and the elucidation of its role in Arabidopsis leaf development have broad implications for auxin-regulated developmental process...
  52. pmc The histone H3K79 methyltransferase Dot1L is essential for mammalian development and heterochromatin structure
    Brendan Jones
    Epigenetics Program, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America
    PLoS Genet 4:e1000190. 2008
    ..Taken together, these results indicate that Dot1L and H3K79 methylation play important roles in heterochromatin formation and in embryonic development...
  53. ncbi The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC
    Michael D Horwich
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 17:1265-72. 2007
    ..2'-O-methylation of siRNAs may be the final step in assembly of the RNAi-enzyme complex, RISC, occurring after an Argonaute-bound siRNA duplex is converted to single-stranded RNA...
  54. pmc Histone methyltransferases regulating rRNA gene dose and dosage control in Arabidopsis
    Frederic Pontvianne
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    Genes Dev 26:945-57. 2012
    ..suecica. H3K9 methyltransferases are also required for variant-specific silencing in A...
  55. ncbi The biosynthesis of adenosylcobalamin (vitamin B12)
    Martin J Warren
    School of Biological Sciences, Queen Mary, University of London, Mile End Road, London, UK E1 4NS
    Nat Prod Rep 19:390-412. 2002
    ..This review covers some of the most important findings that have been made and provides the reader with a complete description of the transformation of uroporphyrinogen III into adenosylcobalamin (AdoCbl). 183 references are cited...
  56. ncbi Methylation of translation-associated proteins in Saccharomyces cerevisiae: Identification of methylated lysines and their methyltransferases
    Timothy A Couttas
    Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, NSW, Australia
    Proteomics 12:960-72. 2012
    This study aimed to identify sites of lysine methylation in Saccharomyces cerevisiae and the associated methyltransferases. Hexapeptide ligand affinity chromatography was used to normalize the abundance levels of proteins in whole cell ..
  57. ncbi The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast
    Takatomi Yamada
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 20:173-85. 2005
    ....
  58. ncbi Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production
    Perng Kuang Chang
    Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, New Orleans, LA 70124, United States
    Fungal Biol 116:298-307. 2012
    ..The loss of hydrophobicity and the other developmental changes in the laeA deletion mutants could affect the ability of the fungus to produce aflatoxins...
  59. pmc Structural basis for the product specificity of histone lysine methyltransferases
    Xing Zhang
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Mol Cell 12:177-85. 2003
    ....
  60. pmc RAM/Fam103a1 is required for mRNA cap methylation
    Thomas Gonatopoulos-Pournatzis
    Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Mol Cell 44:585-96. 2011
    ..Our findings demonstrate that RAM is an essential component of the core gene expression machinery...
  61. ncbi Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases
    D Guo
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA
    Transgenic Res 10:457-64. 2001
    ..The results indicate that both lignin content and composition affect digestibility of alfalfa forage, and reveal a new strategy for forage quality improvement by genetic manipulation of CCoAOMT expression...
  62. pmc DNA methyltransferases are required to induce heterochromatic re-replication in Arabidopsis
    Hume Stroud
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS Genet 8:e1002808. 2012
    ..Hence our results suggest a complex relationship between ATXR5/6 and DNA methylation in the regulation of DNA replication and transcription of TEs...
  63. pmc The last rRNA methyltransferase of E. coli revealed: the yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA
    Anna Y Golovina
    Lomonosov Moscow State University, Department of Chemistry and A N Belozersky Institute of Physico Chemical Biology, Moscow, 119992, Russia
    RNA 18:1725-34. 2012
    The ribosomal RNA (rRNA) of Escherichia coli contains 24 methylated residues. A set of 22 methyltransferases responsible for modification of 23 residues has been described previously...
  64. pmc A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin
    Gunnar Schotta
    Research Institute of Molecular Pathology IMP, The Vienna Biocenter, A 1030 Vienna, Austria
    Genes Dev 18:1251-62. 2004
    ..Together, our data indicate a function for H4-K20 trimethylation in gene silencing and further suggest H3-K9 and H4-K20 trimethylation as important components of a repressive pathway that can index pericentric heterochromatin...
  65. pmc Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases
    Yannick Jacob
    Department of Biology, Indiana University, 915 East Third Street, Bloomington, Indiana 47405, USA
    Nature 466:987-91. 2010
    ..Mutation of ATXR5 and ATXR6 also causes upregulation of transposon expression and has pleiotropic effects on plant development. These results uncover a novel pathway that prevents over-replication of heterochromatin in Arabidopsis...
  66. pmc Genetic association between intronic variants in AS3MT and arsenic methylation efficiency is focused on a large linkage disequilibrium cluster in chromosome 10
    Paulina Gomez-Rubio
    Department of Pharmacology and Toxicology, University of Arizona, Tucson, Arizona 85721 0207, USA
    J Appl Toxicol 30:260-70. 2010
    ..The results reported here should be carefully considered for future genomic association studies involving AS3MT and arsenic metabolism...
  67. pmc Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends
    Kuniaki Saito
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    Genes Dev 21:1603-8. 2007
    ..Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2'-O-methylation in Drosophila...
  68. pmc Requirement for Dot1l in murine postnatal hematopoiesis and leukemogenesis by MLL translocation
    Stephanie Y Jo
    Department of Pathology, University of Michigan, Ann Arbor, MI, USA
    Blood 117:4759-68. 2011
    ..These findings illustrate a crucial role of Dot1l in normal hematopoiesis and leukemogenesis of specific oncogenes...
  69. ncbi A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol
    Shan Lin
    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 277:10795-803. 2002
    ..Because methylation of arsenic is a critical feature of its metabolism, characterization of this enzyme will improve our understanding of this metalloid's metabolism and its actions as a toxin and a carcinogen...
  70. ncbi The mmaA2 gene of Mycobacterium tuberculosis encodes the distal cyclopropane synthase of the alpha-mycolic acid
    Michael S Glickman
    Division of Infectious Diseases, Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 278:7844-9. 2003
    ..These results expand our knowledge of the biosynthesis of the Mtb cell envelope and will allow further elucidation of the relationship between Mtb pathogenesis and the fine structure of mycolic acids...
  71. pmc A Suv39h-dependent mechanism for silencing S-phase genes in differentiating but not in cycling cells
    Slimane Ait-Si-Ali
    UPR 9079 CNRS Labellisée Ligue Nationale Contre le Cancer, Institut Andre Lwoff, Villejuif, France
    EMBO J 23:605-15. 2004
    ..Thus, appropriately timed H3K9 methylation by Suv39h seems to be part of the control switch for exiting the cell cycle and entering differentiation...
  72. pmc Locus-specific control of DNA methylation by the Arabidopsis SUVH5 histone methyltransferase
    Michelle L Ebbs
    Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    Plant Cell 18:1166-76. 2006
    ..The suvh4 suvh5 suvh6 mutant also displays a loss of non-CG methylation similar to a cmt3 mutant, indicating that SUVH4, SUVH5, and SUVH6 together control CMT3 activity...
  73. pmc Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro
    Dmitry Litonin
    Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 285:18129-33. 2010
    ..Thus, only two essential initiation factors, TFAM and TFB2M, and two promoters, LSP and HSP1, are required to drive transcription of the mitochondrial genome...
  74. pmc Tandem repeats upstream of the Arabidopsis endogene SDC recruit non-CG DNA methylation and initiate siRNA spreading
    Ian R Henderson
    Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Genes Dev 22:1597-606. 2008
    ..In addition to revealing the complexity of DNA methylation control in A. thaliana, SDC has important implications for how plant genomes utilize gene silencing to repress endogenous genes...
  75. pmc Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase
    Xing Zhang
    Department of Biochemistry, School of Medicine, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 111:117-27. 2002
    ..This SET domain protein bears no structural similarity to previously characterized AdoMet-dependent methyltransferases but includes notable features such as a triangular Zn3Cys9 zinc cluster in the pre-SET domain and a AdoMet ..
  76. pmc Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes
    Judd C Rice
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    Genes Dev 16:2225-30. 2002
    ..These data suggest that H4 Lys 20 methylation by PR-Set7 during mitosis acts to antagonize H4 Lys 16 acetylation and to establish a mechanism by which this mark is epigenetically transmitted...
  77. pmc Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene
    Mary J Clancy
    Department of Biological Sciences, University of New Orleans, New Orleans, LA 70148, USA
    Nucleic Acids Res 30:4509-18. 2002
    ....
  78. ncbi Active site in RrmJ, a heat shock-induced methyltransferase
    Jutta Hager
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor 48109 1048, USA
    J Biol Chem 277:41978-86. 2002
    ..Based on these results, a reaction mechanism for the methyltransfer activity of RrmJ is proposed...
  79. pmc Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA
    Keith Connolly
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Microbiol 70:1062-75. 2008
    ..These new findings and this proposed regulatory role offer a mechanistic explanation for the extreme conservation of the KsgA/Dim1p enzyme family...
  80. pmc Functional and physical interaction between the histone methyl transferase Suv39H1 and histone deacetylases
    Olivier Vaute
    Laboratoire de Biologie Moleculaire Eucaryote, CNRS UMR 5099, 118 route de Narbonne, 31062 Toulouse, France
    Nucleic Acids Res 30:475-81. 2002
    ..Taken together, our results suggest that a complex containing both the Suv39H1 histone methyl transferase and histone deacetylases could be involved in heterochromatin silencing or transcriptional repression by Rb...
  81. ncbi Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability
    A H Peters
    Research Institute of Molecular Pathology IMP, Vienna Biocenter, Dr Bohrgasse 7, A 1030, Vienna, Austria
    Cell 107:323-37. 2001
    ..Here, we show that the murine Suv39h histone methyltransferases (HMTases) govern H3-K9 methylation at pericentric heterochromatin and induce a specialized histone ..
  82. pmc Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
    Satoshi Kimura
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bldg 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 38:1341-52. 2010
    ..coli that are required for m(4)Cm formation. mraW (renamed rsmH) and yraL (renamed rsmI) encode methyltransferases responsible for the N(4) and 2'-O-methylations of C1402, respectively...
  83. ncbi Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis
    Chih Chin Huang
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
    J Biol Chem 277:11559-69. 2002
    ..65-A resolution, respectively. All three enzymes have a seven-stranded alpha/beta fold similar to other methyltransferases with the location and interactions with the cofactor S-adenosyl-l-methionine conserved...
  84. pmc Early mammalian erythropoiesis requires the Dot1L methyltransferase
    Yi Feng
    Department of Pathology and Laboratory Medicine, Division of Cancer and Developmental Biology, Institute for Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, Kansas City, KS, USA
    Blood 116:4483-91. 2010
    ..1 transcription, thus controlling numbers of circulating erythroid and myeloid cells...
  85. ncbi Ty3/gypsy-like retrotransposon knockout of a 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase is non-lethal, uncovers a cryptic paralogous mutation, and produces novel tocopherol (vitamin E) profiles in sunflower
    Shunxue Tang
    Center for Applied Genetic Technologies, The University of Georgia, 111 Riverbend Road, Athens, GA 30602, USA
    Theor Appl Genet 113:783-99. 2006
    ..MT-2 compensated for the loss of the MT-1 function, and the MT-2 mutation profoundly affected the synthesis of tocopherols without adversely affecting the synthesis of plastoquinone crucial for normal plant growth and development...
  86. pmc NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein
    Christine E Schaner Tooley
    Department of Microbiology, Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Nature 466:1125-8. 2010
    ..Knockdown of NRMT recapitulates the multi-spindle phenotype seen with methylation-defective RCC1 mutants, demonstrating the importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation...
  87. pmc Mutations in gidB confer low-level streptomycin resistance in Mycobacterium tuberculosis
    Sharon Y Wong
    Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Antimicrob Agents Chemother 55:2515-22. 2011
    ..This report provides the first microbiological evidence for the contribution of gidB in streptomycin resistance and examines the clinical implications of mutations in the gidB gene...
  88. ncbi Characterization of an Arabidopsis mutant deficient in gamma-tocopherol methyltransferase
    Eveline Bergmuller
    Max Planck Institute of Molecular Plant Physiology, Department of Lothar Willmitzer, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Mol Biol 52:1181-90. 2003
    ..Therefore, a shift in tocopherol composition or the absence of tocopherol has no major impact on the amounts of specific fatty acids or on lipid hydrolysis...
  89. ncbi MidA is a putative methyltransferase that is required for mitochondrial complex I function
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
    J Cell Sci 123:1674-83. 2010
    ..We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology...
  90. pmc Histone H3 lysine 79 methyltransferase Dot1 is required for immortalization by MLL oncogenes
    Ming Jin Chang
    Department of Biochemistry, Tulane University, New Orleans, Louisiana, USA
    Cancer Res 70:10234-42. 2010
    ..In summary, our data point to a pivotal requirement for Dot1 in MLL fusion protein-mediated leukemogenesis and implicate Dot1 as a potential therapeutic target...
  91. pmc Reconstitution of diphthine synthase activity in vitro
    Xuling Zhu
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Biochemistry 49:9649-57. 2010
    ..Interestingly, the trimethylated product from the PhDph5-catalyzed reaction can easily eliminate the trimethylamino group. The potential implication of this unexpected finding on the diphthamide biosynthesis pathway is discussed...
  92. pmc Emergence of Klebsiella pneumoniae coproducing KPC-2 and 16S rRNA methylase ArmA in Poland
    Katarzyna Zacharczuk
    Department of Bacteriology, National Institute of Public Health National Institute of Hygiene, Warsaw, Poland
    Antimicrob Agents Chemother 55:443-6. 2011
    ..XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of the isolates. All the isolates were resistant to carbapenems and 4,6-disubstituted 2-deoxystreptamines...
  93. ncbi Prevalence of β-lactamases and 16S rRNA methylase genes amongst clinical Klebsiella pneumoniae isolates carrying plasmid-mediated quinolone resistance determinants
    Yanping Luo
    Department of Microbiology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China
    Int J Antimicrob Agents 37:352-5. 2011
    ..qepA was rarely detected in this study...
  94. pmc Cyclopropane fatty acid synthase mutants of probiotic human-derived Lactobacillus reuteri are defective in TNF inhibition
    Sara E Jones
    Interdepartmental Program of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA
    Gut Microbes 2:69-79. 2011
    ..By increasing our understanding of immunomodulation by probiotic species, beneficial microbes can be rationally selected to alleviate intestinal inflammation...
  95. pmc Independent recruitment of an O-methyltransferase for syringyl lignin biosynthesis in Selaginella moellendorffii
    Jing Ke Weng
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Plant Cell 23:2708-24. 2011
    ..moellendorffii. Phylogenetic analysis reveals that Sm COMT, like F5H, evolved independently from its angiosperm counterparts...
  96. ncbi Aspergillus oryzae laeA regulates kojic acid synthesis genes
    Ken Oda
    Genome Biotechnology Laboratory, Kanazawa Institute of Technology
    Biosci Biotechnol Biochem 75:1832-4. 2011
    ..Real-time PCR also confirmed that expression of kojic acid biosynthesis genes decreased in the laeA disruption strain, indicating that these genes are under the control of LaeA...
  97. pmc Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex
    Yu Chen
    State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, P R China
    PLoS Pathog 7:e1002294. 2011
    ..SARS coronavirus (SARS-CoV) encodes two S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTase) which sequentially methylate the RNA cap at guanosine-N7 and ribose 2'-O positions, catalyzed by ..
  98. pmc Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure
    Dmitry V Lesnyak
    Department of Bioinformatics and Bioengineering, Moscow State University, Moscow 119992, Russia
    J Biol Chem 282:5880-7. 2007
    ..Based on the experimental data and current nomenclature the protein expressed from the yhhF gene was renamed to RsmD. A model for interaction of RsmD with ribosome has been proposed...
  99. pmc Transcriptional profiling of methyltransferase genes during growth of Methanosarcina mazei on trimethylamine
    Christian Krätzer
    Institut für Mikrobiologie und Biotechnologie, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany
    J Bacteriol 191:5108-15. 2009
    ..The genes of the mtb1-mtt1 operon encode methyltransferases that are responsible for sequential demethylation of trimethylamine...
  100. ncbi Bluetongue virus VP4 is an RNA-capping assembly line
    Geoff Sutton
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford, OX3 7BN, UK
    Nat Struct Mol Biol 14:449-51. 2007
    ....
  101. pmc RNAi, DRD1, and histone methylation actively target developmentally important non-CG DNA methylation in arabidopsis
    Simon W L Chan
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, USA
    PLoS Genet 2:e83. 2006
    ..intersecting pathways that control the locus-specific patterning of non-CG methylation by the DRM2 and CMT3 methyltransferases. Our results suggest that non-CG DNA methylation that is inherited via a network of persistent targeting ..

Research Grants73

  1. Regulation of Airway Mucin Gene Expression by Epigenetic Mechanism
    Reen Wu; Fiscal Year: 2013
    ..Using siRNA as well as overexpression approaches, the effects of DNA methyltransferases (DNMTs) on CpG island methylation status and MUC5AC expression will be established...
  2. MECHANISMS OF RADIORESISTANCE AND CELL CYCLE PROGRESSION
    Howard B Lieberman; Fiscal Year: 2013
    ..We found that DNA methyltransferases DNMT1 and DNMT3b levels are aberrantly high in several prostate cancer cell lines, and can methylate a "..
  3. CPG METHYLATION AND MUTATION
    Gerd P Pfeifer; Fiscal Year: 2013
    ..repression complex present in stem cells at specific gene loci accelerates or facilitates recruitment of DNA methyltransferases and de novo methylation of CpG-rich sequences during the process of cell transformation...
  4. Signaling Mechanisms in Alcohol Drinking Behaviors
    Subhash C Pandey; Fiscal Year: 2013
    ..DNA methyltransferases (DNMT) catalyze DNA methylation allowing for the binding of methyl-CpG-binding proteins and recruitment of ..
  5. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ..DNA * methyltransferases and histone deacetylases) using interfering RNA technology;3) modulating epigenetic * regulatory components ..
  6. Analysis of epigenetic regulation in early mammalian embryos via RNA interference
    Charles R Long; Fiscal Year: 2010
    ..Our hypothesis states that subtle alterations of histone and DNA methyltransferases during the critical period of in vitro embryo culture manifest in aberrant embryo development...
  7. Transgenerational effects of maternal high fat diet during pregnancy on breast ca
    Leena A Hilakivi-Clarke; Fiscal Year: 2013
    ..The increase in DNA methylation may be caused by up-regulation of DNA methyltransferases (DNMT1, DNMT3a and DNMT3b) and polycombs (EZH2, SUZ12), which we and others have found to occur in the ..
  8. Validation of microRNAs as therapeutic targets in hematological malignancies
    Natarajan Muthusamy; Fiscal Year: 2013
    ..progression in chronic lymphocytic leukemia (CLL) and acute myeloid leukemia (AML) through modulation of DNA methyltransferases [DNMT1, DNMT3A and DNMT3B], DNA hypermethylation and gene silencing and miR-181a in tumor suppressor ..
  9. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2013
    ..preliminary data demonstrate that USF1 interacts with large co-activator complexes containing two histone methyltransferases PRMT1 and hSET1...
  10. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven G Clarke; Fiscal Year: 2013
    ..We are especially interested in methyltransferases that are involved in signal transduction and metabolic regulatory pathways where the presence or absence of ..
  11. Histone Methylation: a role in excessive ethanol intake and self-administration
    Jennifer A Rinker; Fiscal Year: 2012
    ..a transcription factor induced by a number of drugs of abuse, in suppressing the activity of histone methyltransferases, G9a and G9a-like protein (GLP), and consequently reducing methylation of histone H3 on lysine 9 (H3K9) in ..
  12. Characterization of an essential mitochondrial rRNA methyltransferase
    Kip E Guja; Fiscal Year: 2013
    ..KsgA and Dim1 methyltransferases dimethylate two adjacent adenine residues in a conserved stem-loop in the small subunit of the bacterial and ..
  13. DNA Methylation Control Hematopoietic Stem Cell Lineage Differentiation
    GRANT ANTHONY CHALLEN; Fiscal Year: 2013
    ..and I hypothesize that HSC lineage fate determination is at least partly controlled by the actions of DNA methyltransferases. In this study I propose to examine the functional effects of the DNA methyltransferases Dnmt3a and Dnmt3b ..
  14. Functional Analysis of the Embryonic Epigenome in a Non-rodent Model
    Mark E Westhusin; Fiscal Year: 2013
    ..Specific Aim 1 is to characterize the expression and activity of DNA methyltransferases (DNMTs), histone methyltransferases (HMTs) and their associated genes during mammalian oocyte maturation and ..
  15. PCAF/GCN5 ACETYLATION ON P53 TRANSACTIVATION
    Shelley L Berger; Fiscal Year: 2013
    ..function to target both p53 and histones in a coordinated fashion, and this may also be the case for lysine methyltransferases. In general, this coordination may lead to interrelated factor/histone modifications that reinforce one ..
  16. NITRIC OXIDE SYNTHASE IN THE JUXTAGLOMERULAR APPARATUS
    Christopher S Wilcox; Fiscal Year: 2013
    ..ADMA is generated intracellularly by protein arginine methyltransferases (PRMTs), metabolized by dimethylarginine dimethylaminohydrolases (DDAHs) and exported by cationic amino acid ..
  17. ALCOHOL AND HYPERPROLACTINEMIA
    DIPAK KUMAR SARKAR; Fiscal Year: 2013
    ..The proposed research tests the hypothesis that alcohol's modulating effect on DNA methyltransferases and/or histone deacetylases makes an epigenetic mark on the D2 receptor gene that reduces D2 receptor ..
  18. Identification of a Molecular Signature for Barrier Insulators
    LAURIE ANN STEINER; Fiscal Year: 2013
    ..We hypothesize that this signature will be composed of the USF proteins, their associated histone methyltransferases (PRMT1, PRMT4, SET7/9), and acetyltransferases (CBP, P300, PCF), as well as an active chromatin structure (..
  19. Diet and Alcohol Induced Epigenetic Changes in Oral Cavity Carcinogenesis Model
    Lorraine J Gudas; Fiscal Year: 2013
    ..cells is influenced by carcinogens, alcohol, and drugs, such as zebularine and 5-aza-2'-deoxycytidine, which have been shown both to inhibit DNA methyltransferases and to reduce tumor incidence in other carcinogenesis models. )
  20. RNA Modification Enzymes: Methyltransferase/psi-synthase
    Robert M Stroud; Fiscal Year: 2012
    ..and catalysis in two of the most abundant families of RNA modifying enzymes: 5-methyl uracil (m5U) methyltransferases (MTases) and pseudouridine synthases (synthases)...
  21. Targeted epigenetic silencing of oncogenic Transcription Factors (PQ18)
    Pilar Blancafort; Fiscal Year: 2013
    ..As a stable repressive mark, DNAme catalyzed by DNA-methyltransferases, is regarded as a key player in epigenetic silencing...
  22. Protein methyltransferases as transcriptional coregulators
    Michael R Stallcup; Fiscal Year: 2013
    ..Knock-out mouse studies of the euchromatin-associated H3 K9 methyltransferases G9a and GLP indicated that these two enzymes are responsible for the majority of mono- and demethylation of ..
  23. Targeting aberrant epigenetics by nanomedicine
    Shujun Liu; Fiscal Year: 2013
    ..We base that hypothesis on the observations that 1) miR29b disrupts all DNA methyltransferases (DNMTs) directly or indirectly in AML thereby leading to DNA hypomethylation...
  24. IL-15 Characterization Through Experimental Immunology
    Michael A Caligiuri; Fiscal Year: 2013
    ..methylation in IL-15-mediated leukemogenesis may be related to alteration of microRNA that regulate DNA methyltransferases. The latter discovery not only promises to provide insight into mechanism of disease but also therapeutic ..
  25. The Contribution of Protein Methylation in Systemic Lupus Erythematosus
    Hester A Doyle; Fiscal Year: 2011
    ..Arginine methylation is catalyzed by various protein arginine methyltransferases. The proposed studies will examine the mechanisms behind how these protein methylation reactions alter ..
  26. Nicotinic receptor stimulation and epigenetic regulation of GABAergic function.
    Alessandro Guidotti; Fiscal Year: 2013
    ..Evidence suggests that this GABAergic neuropathology of SZ brain is accompanied by the overexpression of DNA-methyltransferases 1 and 3a (DNMT1 and DNMT3a) in GABAergic neurons (Costa et al, 2007;Ruzicka et al, 2007;Veldic et al, 2005;..
  27. The Role of DNMT3B in the DNA Methylation of Cancer Cells
    LUCY ANN GODLEY; Fiscal Year: 2012
    ..that cancer cells exhibit aberrant splicing of the DNMT3B gene, which encodes one of the three DNA methyltransferases. The aberrant splicing produces DNMT3B transcripts containing premature stop codons and encoding truncated ..
  28. Transition State Analogues as Modulators of DNA Methylation
    Vern L Schramm; Fiscal Year: 2012
    ..The MTAP inhibitor alters metabolites that are expected to change the ability of DNA methyltransferases to methylate DNA...
  29. DNA Methyltransferase Target Sites in Cancer
    ROCHELLE LEE TIEDEMANN; Fiscal Year: 2013
    ..methylation is a heritable, epigenetic mark that is conferred by a class of enzymes referred to as the DNA methyltransferases (DNMTs)...
  30. Epigenetic regulation of alcoholic steatohepatitis in a mouse model
    CHARLES HOPKINSON HALSTED; Fiscal Year: 2012
    ..of genes involved in other pathways of alcoholic liver injury and fibrosis in ASH and of relevant DNA and histone methyltransferases, acetylases and de-acetylases that are involved in the epigenetic regulation of these pathways.
  31. Quercetin to enhance the bioavailability and activity of green tea polyphenols
    SUSANNE MARGARETE HENNING; Fiscal Year: 2013
    ..The combined administration also significantly decreased the protein expression of methyltransferases and MRP1...
  32. Creating in vivo Chemical Probes for Lysine Methyltransferases G9a and GLP
    Jian Jin; Fiscal Year: 2013
    ..Among the 'writers', closely related lysine methyltransferases G9a and GLP modulate the transcriptional repression of a variety of genes via dimethylation of histone H3 ..
  33. Regulation of a Novel Trypanosome RNA Binding Protein by Arginine Methylation
    Kaylen Lott; Fiscal Year: 2013
    ..We have characterized five protein arg methyltransferases (PRMTs) and identified over 800 arg methylproteins in T. brucei...
  34. The Role of de novo Cytosine DNA Methyltransferases 3a &3b in Gene Methylation
    Ivo Teneng; Fiscal Year: 2013
    ..These activities along with the training received through the studies outlined in this application will provide Ivo with unique strengths, and the ability to integrate molecular biology, bioinformatics, and statistics. ..
  35. GENESIS AND CONSEQUENCES OF ABERRANT DNA METHYLATION
    Paula M Vertino; Fiscal Year: 2013
    ..During the last funding period we have studied the interdependent relationship between DNA methyltransferases and histone modifying complexes, and their role in promoting or preventing epigenetic silencing in cancer...
  36. Regulation and Function of Active DNA Demethylation in Arabidopsis
    Daniel Zilberman; Fiscal Year: 2013
    ..Mutations in DNA methyltransferases and methylcytosine binding protein genes cause genetic disease, and disrupted DNA methylation is a hallmark ..
  37. Regulation of Hematopoietic Progenitors by de novo DNA Methylation
    Margaret Goodell; Fiscal Year: 2013
    ..as a model to study the perturbations of hematologic function that occur when specific alterations in DNA methyltransferases are induced...
  38. Mapping DNA methylation in hippocampal neurons through bisulfite sequencing
    Guoping Fan; Fiscal Year: 2012
    ..methylation in the development and function of cortex and hippocampus, we have examined expression of DNA methyltransferases (Dnmts including Dnmt1, Dnmt3a, and Dnmt3b) in the developing and adult central nervous system (CNS)...
  39. Histone Deacetylases and Kidney Development
    Samir S El Dahr; Fiscal Year: 2010
    ..Moreover, HDACs, along with histone methyltransferases and other histone modifiers, create an "epigenetic code" that is read by transcriptional repressors or ..
  40. Role of Set2 and H3 methylation in chromatin function
    Brian D Strahl; Fiscal Year: 2013
    ..Our long-term goal is to elucidate how lysine-specific histone methyltransferases regulate gene expression and contribute to cellular development and disease...
  41. Mechanisms of PASG-Mediated Senescence in Hematopoietic Development and Leukemia
    ROBERT JOHN ARCECI; Fiscal Year: 2010
    ..This deletion variant loses its ability to facilitate DNA methylation and to interact with DNA methyltransferases, thus suggesting that it may function in a dominant negative fashion...
  42. Chromatin remodeling mechanisms of gene transcription in memory
    Farah D Lubin; Fiscal Year: 2013
    ..AIM 3. NF-[unreadable]B (p65) is a non-histone target of protein lysine methyltransferases, and once methylated NF[unreadable]B can associate with proteins such as G9a/GLP...
  43. MOLECULAR GENETICS OF NUCLEOLAR DOMINANCE
    CRAIG STUART PIKAARD; Fiscal Year: 2012
    ..a de novo DNA methyltransferase, methylcytosine binding domain proteins, histone deacetylases and histone methyltransferases. Importantly, we recently found that rRNA gene silencing is RNA and Dicer-dependent, involving components of ..
  44. Epigenetic Changes in Retinoid Signaling in Oral Cavity Carcinogenesis
    KATARZYNA MARIA MARCINKIEWICZ; Fiscal Year: 2013
    ..I propose that this transcriptional silencing results from the abnormal recruitment of DNA methyltransferases to the RARBeta2 RARE which occurs as a consequence of PRC protein overexpression or greater association of ..
  45. Molecular mechanisms in the regulation of p53 functions by PAD4
    Yanming Wang; Fiscal Year: 2013
    ..g., histone acetyltransferases and protein Arg methyltransferases) and corepressors (e.g...
  46. Biomarkers for Epigenetic Therapy of Lung Cancer
    Mathewos Tessema; Fiscal Year: 2013
    ..Our own findings revealed that a combination therapy with inhibitors of DNA methyltransferases (5-Aza) and histone deacetylase (MS-275) profoundly affected the growth of orthotopic human lung tumors by ..
  47. DNA Methyltrasferase Gene Expression in Colon Cancer
    Stephen B Baylin; Fiscal Year: 2013
    ..We explore a "molecular progression" model wherein these proteins recruit DNA methyltransferases (DNMT's) to gene promoters and the DNMT's, through transcriptional co- repression, may contribute ..
  48. Environmental toxins and stem cell epigenetic remodeling
    Joyce Ellen Ohm; Fiscal Year: 2013
    ..abnormal recruitment of polycomb repressive complexes (PRC), the stress response protein SIRT1, and/or DNA methyltransferases to gene promoter regions with abnormal DNA methylation changes;and Specific Aim 3: Determine whether low-..
  49. Epigenetic regulation of programmed genome instability in O. trifallax
    JOHN RUSSELL BRACHT; Fiscal Year: 2013
    ..of cytosine methylation in Oxytricha genome rearrangements will be analyzed by use of chemical inhibitors of methyltransferases, followed by deep sequencing the DNA from treated cells...
  50. Re-expression of Aberrantly Silenced Genes Induced by Polyamine Analogues
    Robert A Casero; Fiscal Year: 2013
    ..analogue inhibitors of LSD1 alone and in combination with inhibitors of histone deacetylases and/or DNA methyltransferases for their ability to re-express aberrantly silenced genes;2) evaluate the in vivo effectiveness of our LSD1 ..
  51. TRANSMEMBRANE SIGNAL MODULATION BY HYPERTHERMIA
    STUART KEITH CALDERWOOD; Fiscal Year: 2013
    ..activated by this signaling network then recruit histone modifying enzymes (histone acetylases and histone methyltransferases) to heat shock genes and activate the program of HSP synthesis that underlies cellular homeostasis during ..
  52. DNA Methylation: Structures, Functions, and Regulation
    Xiaodong Cheng; Fiscal Year: 2013
    ..Mammalian DNA methyltransferases, Dnmt1 and Dnmt3a, each contain a C-terminal catalytic domain and a largely uncharacterized N-terminal ..
  53. Functions of Histone Ubiquitination and Methylation
    Zu Wen Sun; Fiscal Year: 2010
    ..Our long-term objective is to elucidate how histone ubiquitinases and histone methyltransferases regulate gene expression and control cell growth...
  54. DNA Hypomethylation and Cortical Neuronal Degeneration
    Guoping Fan; Fiscal Year: 2010
    ..For example, we found that the two de novo DNA methyltransferases, Dnmt3a and Dnmt3b, are differentially expressed in neural precursor cells and postmitotic neurons within ..
  55. Barrier Insulators in Erythropoiesis
    Patrick G Gallagher; Fiscal Year: 2013
    ..cells, addressing the hypothesis that these complexes contain proteins of common function, including histone methyltransferases and acetyltransferases, nucleosomal remodeling proteins, and other critical regulatory proteins...
  56. Regulation of DNA Methylation by a Major Folate Enzyme
    KYLE CRAIG STRICKLAND; Fiscal Year: 2012
    ..that FDH has a direct effect on intracellular levels of S- adenosyl methionine, the major substrate for DNA methyltransferases, and (ii) that FDH requires modification with a 4'-phosphopantetheine prosthetic group for its ..
  57. Dietary Folate: Impact on Prostate Tumor Biology, Genetics and Epigenetics
    DOMINIC JAMES SMIRAGLIA; Fiscal Year: 2010
    ..by contributing to biosynthesis of S-adenosylmethionine (SAM), the methyl donor molecule used by DNA methyltransferases. Prostate epithelial cells place an unusually high demand on these metabolic pathways because SAM is pulled ..
  58. The Role of DNA Demethylation by Gadd45b in Memory and Synaptic Plasticity
    FARAZ ALI SULTAN; Fiscal Year: 2013
    ..DNA methyltransferases catalyze active DNA methylation, but virtually no consistent evidence existed for a molecular regulator of ..
  59. Developmental Mechanisms of Sex-Specific Ischemic Sensitivity in Neonatal Brain
    Stephanie J Murphy; Fiscal Year: 2013
    ..neuronal) ischemic sensitivity by altering expression of the miR-29 family and its target genes, de novo DNA methyltransferases 3A and 3B (DNMT3A and 3B), in astrocytes...
  60. Histone Demethylases and Regulation of Chromatin and Transcription in Eukaryotes
    Yang Shi; Fiscal Year: 2013
    ..for the DDR by determining whether demethylases for the tri-methylated H3K4 as well as a host of H3K4 methyltransferases also play a role in the DDR...
  61. ROLE OF DNA METHYLTRANSFERASE 3B IN LUNG TUMORIGENESIS
    Li Mao; Fiscal Year: 2013
    ..Disregulation of DNA methyltransferases, particularly DNMT1 and DNMT3B, has been implicated in the abnormal distribution of DNA methylation patterns ..
  62. ANTI-INFLAMMATORY EFFECTS OF TARGETING THE HDAC6/HSP90 PATHWAY
    WAYNE WILLIAM HANCOCK; Fiscal Year: 2011
    ..remodeling and gene transcription by the inhibition of histone/protein deacetylases (HDACs) and DNA methyltransferases (DNMTs)...
  63. Impact of PAH carcinogen-DNA adducts on DNA methylation
    Nicholas Geacintov; Fiscal Year: 2003
    ..The enzymes involved in this process are DNA methyltransferases (MTases) which recognize CpG sequences in DNA and catalyze the transfer of methyl group from S-adenosyl-L-..
  64. Morphine in Mammals
    MEINHART ZENK; Fiscal Year: 2009
    ..morphine biosynthesis such as those encoding tyrosine hydroxylase, DOPA decarboxylase and catechol O-methyltransferases. Once a brain region is identified, the general gene expression pattern provided in brainatlas...
  65. DNA Methyltransferase Inhibition by Intercalating Agents
    Keith D Robertson; Fiscal Year: 2010
    ..The central hypothesis we plan to test in this application is that inhibition of DNA methyltransferases (DNMTs) by DNA intercalating agents leads to alterations in genomic DNA methylation patterns and contributes ..
  66. Structure and Mechanism of Pathogen SET Domain HKMTs
    Ming Ming Zhou; Fiscal Year: 2009
    ..lysine methylation is almost exclusively catalyzed by a large family of the SET domain histone lysine methyltransferases (HKMTs), some of which have been implicated in human diseases including cancers and leukemia...
  67. Epigenetics of alcohol effects on stress axis development
    DIPAK SARKAR; Fiscal Year: 2009
    ..and by evaluating whether gene-silencing activity is accompanied by the alteration in the expression of DNA methyltransferases in these neurons in the hypothalamus of offspring exposed to alcohol during the developmental period...
  68. AdoHcy Hydrolase Inhibitors as Antiparasitic Agents
    RONALD BORCHARDT; Fiscal Year: 2006
    ..levels of AdoHcy, this enzyme plays a pivotal role in regulating S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases which are crucial for the viability of mammalian cells and parasites (e.g...
  69. Role of histone methyltransferases hDOTIL in leukemogenesis
    Yi Zhang; Fiscal Year: 2009
    ..One such epigenetic modification is histone methylation. Of the histone methyltransferases (HMTases) identified so far, yeast Dot1 and its human counterpart, hDOTIL, are unique due to their lack of ..
  70. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven Clarke; Fiscal Year: 2009
    ..understand the physiological role of several related members of a family of S-adenosylmethionine-dependent methyltransferases in aging, metabolic control, and signal transduction...
  71. 2010 FASEB Conference "Biological Methylation:From DNA to Histones and Beyond"
    Paula M Vertino; Fiscal Year: 2010
    ..Other sessions deal with the structure and function of DNA and protein methyltransferases/ demethylases, and the posttranslational methylation of non-histone proteins, which has emerged as an ..
  72. Reactivation of GSTP1 Gene by Green Tea Polyphenols
    Sanjay Gupta; Fiscal Year: 2005
    ..Since DNA methylation is influenced by the relative activities of DNA methyltransferases (DNMT) and DNA histone deacetylases (HDAC), the innovative idea to be tested in this proposal is that green ..
  73. NOVEL DNA METHYLTRANSFERASES IN DEVELOPMENT AND DISEASE
    Timothy Bestor; Fiscal Year: 2002
    ..We have identified a new family of DNA methyltransferases that are strong candidates for the agents that establish methylation patterns...