hydroxymethyl and formyl transferases

Summary

Summary: Enzymes that catalyze the transfer of hydroxymethyl or formyl groups. EC 2.1.2.

Top Publications

  1. ncbi Polyglutamation of methotrexate with common polymorphisms in reduced folate carrier, aminoimidazole carboxamide ribonucleotide transformylase, and thymidylate synthase are associated with methotrexate effects in rheumatoid arthritis
    Thierry Dervieux
    Prometheus Laboratories, San Diego, California, USA
    Arthritis Rheum 50:2766-74. 2004
  2. pmc Investigation of candidate polymorphisms and disease activity in rheumatoid arthritis patients on methotrexate
    Yvonne C Lee
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Rheumatology (Oxford) 48:613-7. 2009
  3. pmc Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli
    J M Guillon
    Laboratoire de Biochimie, Unité Associée au Centre National de la Recherche Scientifique numéro 240, Ecole Polytechnique, Palaiseau, France
    J Bacteriol 174:4294-301. 1992
  4. ncbi Formylation is not essential for initiation of protein synthesis in all eubacteria
    D T Newton
    Guelph Waterloo Center for Graduate Work in Chemistry and Biochemistry, Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 274:22143-6. 1999
  5. pmc Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA
    Y Li
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 182:2886-92. 2000
  6. ncbi Polymorphisms in folate, pyrimidine, and purine metabolism are associated with efficacy and toxicity of methotrexate in psoriasis
    Emanuela Campalani
    King s College, Skin Therapy Research Unit, St John s Institute of Dermatology, London, UK
    J Invest Dermatol 127:1860-7. 2007
  7. ncbi Risk genotypes in folate-dependent enzymes and their association with methotrexate-related side effects in rheumatoid arthritis
    Michael H Weisman
    Cedars Sinai Medical Center, Los Angeles, California, USA
    Arthritis Rheum 54:607-12. 2006
  8. ncbi A defect in the p53 response pathway induced by de novo purine synthesis inhibition
    Julie L Bronder
    Department of Pharmacology and Toxicology and the Massey Cancer Center, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Biol Chem 278:48861-71. 2003
  9. ncbi A new nonlinear mixture response surface paradigm for the study of synergism: a three drug example
    Donald B White
    Departments of Mathematics and Pharmacology, The University of Toledo, Toledo, OH 43606, USA
    Curr Drug Metab 4:399-409. 2003
  10. ncbi Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
    T Akira
    Department of Food Science and Nutrition, Nara Women s University, Japan
    Gene 197:289-93. 1997

Research Grants

Scientific Experts

Detail Information

Publications165 found, 100 shown here

  1. ncbi Polyglutamation of methotrexate with common polymorphisms in reduced folate carrier, aminoimidazole carboxamide ribonucleotide transformylase, and thymidylate synthase are associated with methotrexate effects in rheumatoid arthritis
    Thierry Dervieux
    Prometheus Laboratories, San Diego, California, USA
    Arthritis Rheum 50:2766-74. 2004
    ....
  2. pmc Investigation of candidate polymorphisms and disease activity in rheumatoid arthritis patients on methotrexate
    Yvonne C Lee
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Rheumatology (Oxford) 48:613-7. 2009
    ..We examined the association between candidate single nucleotide polymorphisms (SNPs) and disease activity in RA patients on MTX...
  3. pmc Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli
    J M Guillon
    Laboratoire de Biochimie, Unité Associée au Centre National de la Recherche Scientifique numéro 240, Ecole Polytechnique, Palaiseau, France
    J Bacteriol 174:4294-301. 1992
    ..At 37 degrees C, in a rich medium, the absence of a functional fmt gene reduced the growth rate to 0.28 doubling per h, from 2.3 for the control strain. At 42 degrees C, the studied fmt mutant strain did not grow further...
  4. ncbi Formylation is not essential for initiation of protein synthesis in all eubacteria
    D T Newton
    Guelph Waterloo Center for Graduate Work in Chemistry and Biochemistry, Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 274:22143-6. 1999
    ..Therefore, the dogma that eubacteria require formylation of the initiator methionyl-tRNA for initiation of protein synthesis may have been an invalid generalization of results obtained with E. coli...
  5. pmc Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA
    Y Li
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 182:2886-92. 2000
    ..The only growth phenotype observed was a longer lag time during growth on nonfermentable carbon sources in minimal media for the mis1 deletion strain but not for the fmt1 deletion strain...
  6. ncbi Polymorphisms in folate, pyrimidine, and purine metabolism are associated with efficacy and toxicity of methotrexate in psoriasis
    Emanuela Campalani
    King s College, Skin Therapy Research Unit, St John s Institute of Dermatology, London, UK
    J Invest Dermatol 127:1860-7. 2007
    ..We have demonstrated preliminary evidence that specific polymorphisms of enzymes involved in folate, pyrimidine, and purine metabolism could be useful in predicting clinical response to methotrexate in patients with psoriasis...
  7. ncbi Risk genotypes in folate-dependent enzymes and their association with methotrexate-related side effects in rheumatoid arthritis
    Michael H Weisman
    Cedars Sinai Medical Center, Los Angeles, California, USA
    Arthritis Rheum 54:607-12. 2006
    ..This study investigated whether risk genotypes for folate-dependent enzymes are associated with the toxicity of MTX in patients with rheumatoid arthritis (RA)...
  8. ncbi A defect in the p53 response pathway induced by de novo purine synthesis inhibition
    Julie L Bronder
    Department of Pharmacology and Toxicology and the Massey Cancer Center, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Biol Chem 278:48861-71. 2003
    ....
  9. ncbi A new nonlinear mixture response surface paradigm for the study of synergism: a three drug example
    Donald B White
    Departments of Mathematics and Pharmacology, The University of Toledo, Toledo, OH 43606, USA
    Curr Drug Metab 4:399-409. 2003
    ....
  10. ncbi Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
    T Akira
    Department of Food Science and Nutrition, Nara Women s University, Japan
    Gene 197:289-93. 1997
    ..Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy...
  11. ncbi The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome
    G Brodsky
    Eleanor Roosevelt Institute, 1899 Gaylord Street, Denver, CO 80206, USA
    Hum Mol Genet 6:2043-50. 1997
    ..In contrast, the GARS and GARS-AIRS-GART proteins continue to be expressed during the postnatal development of the cerebellum in individuals with Down syndrome...
  12. ncbi Tight binding of folate substrates and inhibitors to recombinant mouse glycinamide ribonucleotide formyltransferase
    S P Sanghani
    Department of Pharmacology and Toxicology and the Massey Cancer Center, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    Biochemistry 36:10506-16. 1997
    ....
  13. ncbi Deaza analogs of folic acid as antitumor agents
    R L Kisliuk
    Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111, USA
    Curr Pharm Des 9:2615-25. 2003
    ..These processes can be modulated by competition with folates...
  14. ncbi The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping
    E A Rayl
    Department of Pediatrics, Yale University, New Haven, Connecticut 06510, USA
    J Biol Chem 271:2225-33. 1996
    ..The truncation mutant possessing AICARFT activity displays steady state kinetic parameters identical to those of the holoenzyme...
  15. ncbi Structural insights into the human and avian IMP cyclohydrolase mechanism via crystal structures with the bound XMP inhibitor
    Dennis W Wolan
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 43:1171-83. 2004
    ..Several other residues, including Lys66, Tyr104, Asp125, and Lys137', provide substrate specificity and likely enhance the catalytic rate through contributions to acid-base catalysis...
  16. ncbi Catalytic mechanism of the cyclohydrolase activity of human aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase
    James M Vergis
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Biochemistry 43:1184-92. 2004
    ..From the experiments presented here, a catalytic mechanism for the cyclohydrolase activity is postulated...
  17. ncbi Crystal structures of human bifunctional enzyme aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase in complex with potent sulfonyl-containing antifolates
    Cheom Gil Cheong
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:18034-45. 2004
    ..Potent and selective inhibition of the AICAR transformylase active site, compared with other folate-dependent enzymes, should therefore be pursued by further design of sulfonyl-containing antifolates...
  18. pmc AICA-ribosiduria: a novel, neurologically devastating inborn error of purine biosynthesis caused by mutation of ATIC
    Sandrine Marie
    Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology and Cliniques Universitaires Saint Luc, Universite Catholique de Louvain, Brussels, Belgium
    Am J Hum Genet 74:1276-81. 2004
    ..Sequencing of ATIC showed a K426R change in the transformylase region in one allele and a frameshift in the other. Recombinant protein carrying mutation K426R completely lacks AICAR transformylase activity...
  19. ncbi Structure and functional relationships in human pur H
    G P Beardsley
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520 8064, USA
    Adv Exp Med Biol 431:221-6. 1998
    ..Among these, we have thus far completed studies on two, Lysine 265 and Histidine 266. These appear to be critically involved in the AICARFT reaction, although whether their role(s) are in catalysis or binding remains to be determined...
  20. ncbi 10-(2-benzoxazolcarbonyl)-5,10-dideaza-acyclic-5,6,7,8-tetrahydrofolic acid: a potential inhibitor of GAR transformylase and AICAR transformylase
    Thomas H Marsilje
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 11:4503-9. 2003
    ..Ketone 1 and the corresponding alcohol 13 were evaluated for inhibition of GAR Tfase and AICAR Tfase and the former was found to be a potent inhibitor of recombinant human (rh) GAR Tfase (Ki=600 nM)...
  21. ncbi Molecular structure of Escherichia coli PurT-encoded glycinamide ribonucleotide transformylase
    J B Thoden
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    Biochemistry 39:8791-802. 2000
    ..Strikingly, the amino group of GAR that is formylated during the reaction lies at 2.8 A from one of the gamma-phosphoryl oxygens of the AMPPNP...
  22. ncbi Folate status and the safety profile of antifolates
    Hilary Calvert
    Department of Oncology, University of Newcastle upon Tyne, Newcastle upon Tyne, England
    Semin Oncol 29:3-7. 2002
    ..A policy of nutritional supplementation was introduced and led to a marked reduction in toxicity and the abolition of treatment-related deaths with apparent preservation of anticancer activity...
  23. ncbi Antifolates targeting purine synthesis allow entry of tumor cells into S phase regardless of p53 function
    Julie L Bronder
    Department of Pharmacology and Toxicology, Massey Cancer Center, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Cancer Res 62:5236-41. 2002
    ..We conclude that carcinoma cells are killed equally well by DDATHF and related compounds whether or not the p53 pathway is intact and that the utility of GART inhibitors would not be limited to p53-negative tumors...
  24. ncbi Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR
    Yan Zhang
    Department of Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 41:14206-15. 2002
    ....
  25. ncbi Mycobacterium tuberculosis ketopantoate hydroxymethyltransferase: tetrahydrofolate-independent hydroxymethyltransferase and enolization reactions with alpha-keto acids
    Michele Sugantino
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 42:191-9. 2003
    ..These results are discussed in the context of steric restrictions present in the enzyme active site and the stereochemistry of base-catalyzed isotope exchange...
  26. ncbi Thymidine and hypoxanthine protection patterns of the folic acid-enhanced synergies for combinations of trimetrexate plus a polyglutamylatable inhibitor of purine or thymidylate synthesis against human ileocecal HCT-8 cells
    Hélène M Faessel
    Groton Laboratories, Pfizer Inc, Clinical PK PD, Groton, CT 06340, USA
    Int J Oncol 23:401-9. 2003
    ..These results further substantiate the hypothesis that the nonpolyglutamylatable DHFR inhibitor, TMQ, acts as a modulator by decreasing the protection by PteGlu of cells against the polyglutamylatable AG2034 and RTX...
  27. ncbi Control of expression of one-carbon metabolism genes of Saccharomyces cerevisiae is mediated by a tetrahydrofolate-responsive protein binding to a glycine regulatory region including a core 5'-CTTCTT-3' motif
    S P Hong
    School of Biochemistry and Molecular Genetics, The University of New South Wales, Sydney, New South Wales 2052, Australia
    J Biol Chem 274:10523-32. 1999
    ..Tetrahydrofolate or a derivative may act as a ligand for the transcription factor controlling expression of one-carbon metabolism genes...
  28. ncbi Synthesis and biological activity of 7-oxo substituted analogues of 5-deaza-5,6,7,8-tetrahydrofolic acid (5-DATHF) and 5,10-dideaza-5,6,7,8-tetrahydrofolic acid (DDATHF)
    J I Borrell
    , , Universitat Ramon Llull, Via Augusta 390, E-08017 Barcelona, Spain
    J Med Chem 44:2366-9. 2001
    ..coliGARTFase-5-DATHF-GAR pointed to an electronic repulsion between the atoms of the 7-oxo group and the carbonyl group of Arg90 as a possible explanation for the inactivity of 18a-c...
  29. ncbi Rational design, synthesis, evaluation, and crystal structure of a potent inhibitor of human GAR Tfase: 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
    Yan Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:6043-56. 2003
    ....
  30. ncbi Molecular cloning and sequence analysis of the Schizosaccharomyces pombe ade10+ gene
    C Liedtke
    Institute of Genetics, Technische Universitat Braunschweig, Germany
    Yeast 14:1307-10. 1998
    ..Moreover our data indicate that intrachromosomal recombination in Schiz. pombe is enhanced if the ade10 gene product is defective...
  31. ncbi A pH-dependent stabilization of an active site loop observed from low and high pH crystal structures of mutant monomeric glycinamide ribonucleotide transformylase at 1.8 to 1.9 A
    Y Su
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92093 0359, USA
    J Mol Biol 281:485-99. 1998
    ..Thus, the pH-dependence of the enzyme activity appears to arise from local active site rearrangements and not from differences due to monomer-dimer association...
  32. ncbi Design, synthesis, and biological evaluation of simplified alpha-keto heterocycle, trifluoromethyl ketone, and formyl substituted folate analogues as potential inhibitors of GAR transformylase and AICAR transformylase
    Thomas H Marsilje
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 11:4487-501. 2003
    ....
  33. ncbi The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling
    Karen G Bulock
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:22168-74. 2002
    ..3) There was no kinetic evidence of substrate channeling of the intermediate, the formylaminoimidazole carboxamide ribonucleotide, between the formyltransferase and the cyclohydrolase active sites...
  34. ncbi Mapping of the bovine genes of the de novo AMP synthesis pathway
    T Bønsdorff
    Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, PO Box 8146 Dep, 0033 Oslo, Norway
    Anim Genet 35:438-44. 2004
    ..The seven bovine genes are expressed at a higher level in testicular and ovary tissues compared with skeletal muscle...
  35. pmc On the structural and functional modularity of glycinamide ribonucleotide formyltransferases
    Seung Goo Lee
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Protein Sci 12:2206-14. 2003
    ..The chimeras in general, however, are subject to temperature instability and misfolding; thus, they serve primarily as useful candidates for further rounds of optimization...
  36. ncbi Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia
    Kazuko Okamura-Ikeda
    Institute for Enzyme Research, The University of Tokushima, Tokushima 770 8503, Japan
    J Mol Biol 351:1146-59. 2005
    ..A clever mechanism of recruiting the aminomethyl lipoate arm to the reaction site seems to function as a way of avoiding the release of toxic formaldehyde...
  37. ncbi De novo purine nucleotide biosynthesis: cloning, sequencing and expression of a chicken PurH cDNA encoding 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase
    L Ni
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907
    Gene 106:197-205. 1991
    ..Large quantities of purified enzyme and the ability to generate site-directed mutations should make mechanistic studies possible. The recombinant enzyme also affords a simple and reliable approach to identifying new antifolates...
  38. pmc Modular organization of FDH: Exploring the basis of hydrolase catalysis
    Steven N Reuland
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Protein Sci 15:1076-84. 2006
    ..Our results further imply that Ile in place of Asn in the FDH hydrolase catalytic center is an important determinant for hydrolase catalysis as opposed to transferase catalysis...
  39. ncbi ABCB1 C3435T polymorphism influences methotrexate sensitivity in rheumatoid arthritis patients
    R Takatori
    Dept of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Clin Exp Rheumatol 24:546-54. 2006
    ....
  40. doi Crystal structure of AICAR transformylase IMP cyclohydrolase (TM1249) from Thermotoga maritima at 1.88 A resolution
    Herbert L Axelrod
    Joint Center for Structural Genomics JCSG, The Scripps Research Institute, BCC206, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proteins 71:1042-9. 2008
  41. ncbi Developmental changes in the folate-dependent enzymes of de novo purine biosynthesis in rat brain
    J Dominguez
    J Neurochem 38:625-30. 1982
    ..Our findings lend strong support to the suggestion that folic acid-dependent metabolism plays an important role during early development of the brain...
  42. pmc A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase
    You Na Kang
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 46:5050-62. 2007
    ....
  43. ncbi Associations of common polymorphisms in the thymidylate synthase, reduced folate carrier and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase genes with folate and homocysteine levels and venous thrombosis
    Henkjan Gellekink
    Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Clin Chem Lab Med 45:471-6. 2007
    ..Genetic variation in folate-metabolizing genes may affect folate availability and hence confer a greater risk of venous thrombosis...
  44. ncbi A clinical pharmacogenetic model to predict the efficacy of methotrexate monotherapy in recent-onset rheumatoid arthritis
    Judith A M Wessels
    Leiden University Medical Center, Leiden, The Netherlands
    Arthritis Rheum 56:1765-75. 2007
    ..To develop a clinical pharmacogenetic model to predict the efficacy of methotrexate (MTX) in rheumatoid arthritis (RA)...
  45. ncbi The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase
    Tanya E S Dahms
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 44:9841-50. 2005
    ....
  46. pmc Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistance
    Petia Z Gatzeva-Topalova
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Structure 13:929-42. 2005
    ..The proposed mechanism for ArnA_DH has important implications for the design of selective inhibitors...
  47. ncbi Synthesis and biological evaluation of alpha- and gamma-carboxamide derivatives of 10-CF3CO-DDACTHF
    Youhoon Chong
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 13:3587-92. 2005
    ..Like 1, 2 is transported into the cell by the reduced folate carrier. Unlike 1, the functional activity of 2 is not dependent upon FPGS polyglutamylation...
  48. ncbi Design, synthesis, and biological evaluation of 10-methanesulfonyl-DDACTHF, 10-methanesulfonyl-5-DACTHF, and 10-methylthio-DDACTHF as potent inhibitors of GAR Tfase and the de novo purine biosynthetic pathway
    Heng Cheng
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 13:3577-85. 2005
    ..0 microM and 2, IC50 = 2.0 microM)...
  49. ncbi Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus
    K A Flannigan
    Seattle Biomedical Research Institute, Washington 98109
    Mol Microbiol 4:381-92. 1990
    ..Regulation by the purR gene product was explained by the discovery of a purR binding site in the purHD control region...
  50. ncbi Crystal structure of a component of glycine cleavage system: T-protein from Pyrococcus horikoshii OT3 at 1.5 A resolution
    Neratur K Lokanath
    Highthroughput Factory, RIKEN Harima Institute at SPring 8, Hyogo, Japan
    Proteins 58:769-73. 2005
  51. ncbi Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase
    S E Greasley
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 40:13538-47. 2001
    ..Thus, the crystal structure revealed the successful formation of an enzyme-assembled multisubstrate adduct and highlighted a potential application for epoxides, and perhaps aziridines, in the design of efficacious GAR Tfase inhibitors...
  52. ncbi Crystal structure at 2.4 A resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate
    J N Scarsdale
    Department of Biochemistry and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 E Leigh St Suite 212, Richmond, VA 23219, USA
    J Mol Biol 296:155-68. 2000
    ..coli enzymes. The structure of the E. coli ternary complex provides the basis for a thorough investigation of its mechanism through characterization and structure determination of site mutants...
  53. ncbi Function of the active-site lysine in Escherichia coli serine hydroxymethyltransferase
    D Schirch
    Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298
    J Biol Chem 268:23132-8. 1993
    ....
  54. ncbi Antifolates induce inhibition of amido phosphoribosyltransferase in leukemia cells
    M E Sant
    Department of Biochemistry, University of Sydney, New South Wales, Australia
    J Biol Chem 267:11038-45. 1992
    ..41 +/- 0.08 microM for interaction with the enzyme-glutamine complex in vitro. Folate-Glu5, MTX-Glu5, PTX, dihydrotriazine benzenesulfonyl fluoride, and AICAR also inhibit amido phosphoribosyltransferase...
  55. ncbi Genes encoding synthetases of cyclic depsipeptides, anabaenopeptilides, in Anabaena strain 90
    L Rouhiainen
    Department of Applied Chemistry and Microbiology and Institute of Biotechnology, PO Box 56, Biocenter Viikki, FIN 00014 Helsinki University, Finland
    Mol Microbiol 37:156-67. 2000
    ..Mass spectrometry analysis showed that anabaenopeptilides are not made in the mutant strain, but other peptides, such as microcystins and anabaenopeptins, are still produced by the mutant...
  56. ncbi Serine hydroxymethyltransferase isoforms are differentially inhibited by leucovorin: characterization and comparison of recombinant zebrafish serine hydroxymethyltransferases
    Wen Ni Chang
    Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, No 1, University Road, Tainan 701, Taiwan
    Drug Metab Dispos 35:2127-37. 2007
    ..Together with the previous report on zebrafish cytosolic SHMT, we suggest that zSHMTs can be used in in vitro assays for folate-related investigation and antifolate drug discovery...
  57. ncbi In vitro reconstituted biotransformation of 4-fluorothreonine from fluoride ion: application of the fluorinase
    Hai Deng
    School of Chemistry and Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Chem Biol 15:1268-76. 2008
    ..The biotransformation demonstrates the power of the fluorinase to initiate C-F bond formation for organo-fluorine synthesis...
  58. ncbi Glycine metabolism in Candida albicans: characterization of the serine hydroxymethyltransferase (SHM1, SHM2) and threonine aldolase (GLY1) genes
    J B McNeil
    Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3
    Yeast 16:167-75. 2000
    ..cerevisiae. GenBank Accession Nos of the C. albicans SHM1 and SHM2 are AF009965 and AF009966, respectively. Accession No. for C. albicans GLY1 is AF009967...
  59. ncbi Metabolism-blocked antifolates as potential anti-rheumatoid arthritis agents: 4-amino-4-deoxy-5,8,10-trideazapteroyl-d,l-4'-methyleneglutamic acid (CH-1504) and its analogs
    John J McGuire
    Grace Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, NY 14263, United States
    Biochem Pharmacol 77:1161-72. 2009
    ..Overall, these data provide several interesting new leads for preclinical development...
  60. ncbi Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis
    N Monschau
    Institut für Biotechnologie 1, Forschungszentrum Julich, Germany
    FEMS Microbiol Lett 150:55-60. 1997
    ..On ethanol as carbon source simultaneous disruption of GLY1, SHM1 and SHM2 did not lead to glycine auxotrophy because glycine biosynthesis proceeds via alanine glyoxylate aminotransferase...
  61. ncbi Cloning and characterization of methenyltetrahydrofolate synthetase from Saccharomyces cerevisiae
    William B Holmes
    Department of Chemistry and Biochemistry, The Institute for Cellular and Molecular Biology, and the Biochemical Institute, University of Texas, Austin 78712, USA
    J Biol Chem 277:20205-13. 2002
    ..Growth phenotypes of multiply disrupted strains are consistent with a regulatory role for 5-CHO-THF in one-carbon metabolism and additionally suggest a metabolic interaction between the purine and methionine pathways...
  62. ncbi Cloning of human cDNAs encoding mitochondrial and cytosolic serine hydroxymethyltransferases and chromosomal localization
    T A Garrow
    Department of Nutritional Sciences, University of California, Berkeley 94720
    J Biol Chem 268:11910-6. 1993
    ..The high degree of nucleotide sequence identity between the two isozymes, and the presence of keratin genes in both chromosomal regions, is consistent with these regions of chromosome 12 and 17 arising by a duplication event...
  63. ncbi Clinical pharmacogenetic model to predict response of MTX monotherapy in patients with established rheumatoid arthritis after DMARD failure
    Jaap Fransen
    Radboud University Nijmegen Medical Center, PO Box 9101, 6500HB Nijmegen, The Netherlands
    Pharmacogenomics 13:1087-94. 2012
    ..The performance of a clinical pharmacogenetic model to predict nonresponse of methotrexate (MTX) monotherapy in patients with established rheumatoid arthritis (RA) and failure of disease-modifying antirheumatic drugs (DMARDs) was studied...
  64. ncbi Cellular responses to L-serine in Saccharomyces cerevisiae: roles of general amino acid control, compartmentalization, and aspartate synthesis
    Johnny C Y Lee
    Ramaciotti Center for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    FEMS Yeast Res 13:618-34. 2013
    ..L-aspartate synthesis was exclusively through mitochondrial metabolism of L-serine to pyruvate and ammonia, involving Cha1p, cytoplasmic pyruvate carboxylases Pyc1p and Pyc2p, and the cytoplasmic aspartate aminotransferase Aat2p...
  65. pmc Elevated levels of ketopantoate hydroxymethyltransferase (PanB) lead to a physiologically significant coenzyme A elevation in Salmonella enterica serovar Typhimurium
    Aileen Rubio
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 184:2827-32. 2002
    ..Furthermore, overexpression of PanB caused by this mutation, or by other means, was necessary and sufficient to increase pantothenate production and allow PurF-independent thiamine synthesis on glucose medium...
  66. ncbi The crystal structure of archaeal serine hydroxymethyltransferase reveals idiosyncratic features likely required to withstand high temperatures
    Francesco Angelucci
    Department of Life, Health and Environmental Sciences, University of L Aquila, P le Salvatore Tommasi 1, L Aquila, Italy
    Proteins 82:3437-49. 2014
    ..Both peculiar features may be tested by designing enzyme variants potentially endowed with improved stability for applications in biomimetic processes. Proteins 2014; 82:3437-3449. © 2014 Wiley Periodicals, Inc. ..
  67. pmc Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
    Timothy H P Tan
    Department of Biology Zoology, University of Fribourg, Chemin du Musee 10, CH 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 99:1152-7. 2002
    ..Whereas the predicted protein is homologous to prokaryotic and mitochondrial methionyl-tRNA(Met) formyltransferases, it has about twice the mass of any of these proteins...
  68. ncbi Assignment of more than one gene to the same chromosome band on metaphase cytogenetics
    L H C Tan
    Histopathology 44:623-4; author reply 625. 2004
  69. ncbi Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2
    Anne Wohlke
    Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17 p, 30559 Hannover, Germany
    Gene 348:73-81. 2005
    ..Comparative genome analysis between cattle, human and mouse indicates that the chromosomal location of the bovine GART gene is in agreement with a previously published mapping report...
  70. ncbi An enzymatic pathway for the biosynthesis of the formylhydroxyornithine required for rhodochelin iron coordination
    Mattia Bosello
    Biochemistry, Department of Chemistry, Philipps University Marburg, Hans Meerwein Strasse, D 35043 Marburg, Germany
    Biochemistry 51:3059-66. 2012
    ..2004) Arch. Microbiol.182, 313-325; Pohlmann, V. (2008) Org. Biomol. Chem.6, 1843-1848]...
  71. ncbi Structure of E. coli ketopantoate hydroxymethyl transferase complexed with ketopantoate and Mg2+, solved by locating 160 selenomethionine sites
    Frank von Delft
    Department of Biochemistry, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Structure 11:985-96. 2003
    ..The active site contains a ketopantoate bidentately coordinated to Mg(2+). Similar binding is likely for the substrate, alpha-ketoisovalerate, orienting the C3 for deprotonation...
  72. ncbi Structures and reaction mechanisms of the two related enzymes, PurN and PurU
    Gen Ichi Sampei
    Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro Communications, 1 5 1 Chofugaoka, Chofu, Tokyo 182 8585, Japan RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo, Sayo, Hyogo 679 5148, Japan Structural Biology Group, SPring 8 JASRI, 1 1 1 Kouto, Sayo, Sayo, Hyogo 679 5198, Japan Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2 17 1 Tsudanuma, Narashino, Chiba 275 0016, Japan and Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    J Biochem 154:569-79. 2013
    ..The evolutionary relationship of the two enzymes is discussed based on the comparisons of the structures and the catalytic mechanisms. ..
  73. pmc The enzymatic activity of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase is enhanced by NPM-ALK: new insights in ALK-mediated pathogenesis and the treatment of ALCL
    Francesco E Boccalatte
    Center for Experimental Research and Medical Studies CERMS, University of Torino, Via Santena 5, 10126 Torino, Italy
    Blood 113:2776-90. 2009
    ..Prediction of tumor responses to methotrexate may justify specific molecular-based chemotherapy...
  74. ncbi [The second half of de novo synthetic pathway of IMP and conversion of IMP to AMP]
    Jun Oka
    National Institute of Health and Nutrition
    Nihon Rinsho 61:52-8. 2003
  75. pmc Comparative analysis of the Escherichia coli ketopantoate hydroxymethyltransferase crystal structure confirms that it is a member of the (betaalpha)8 phosphoenolpyruvate/pyruvate superfamily
    Florian Schmitzberger
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Bacteriol 185:4163-71. 2003
    ..Based on the analysis, we propose that in this superfamily there should be a subdivision into two groups. This paper completes our structural analysis of the E. coli enzymes in the pantothenate pathway...
  76. ncbi A proteomics approach to study in vivo protein N(alpha)-modifications
    Xumin Zhang
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
    J Proteomics 73:240-51. 2009
    ..Our results suggest that N-terminal acetyltransferases (NATs) may function as N-terminal formyltransferases (NFTs) and N-terminal propionyltransferases (NPTs) in vivo...
  77. ncbi Structural insights into the avian AICAR transformylase mechanism
    Dennis W Wolan
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochemistry 41:15505-13. 2002
    ..Lys(267) is likely to be involved in the stabilization of the anionic formyl transfer transition state and in subsequent protonation of the THF leaving group...
  78. ncbi High-level production of tetraacetyl phytosphingosine (TAPS) by combined genetic engineering of sphingoid base biosynthesis and L-serine availability in the non-conventional yeast Pichia ciferrii
    Christoph Schorsch
    Goethe University Frankfurt, Institute of Molecular Biosciences, Max von Laue Strasse 9, Frankfurt, Germany
    Metab Eng 14:172-84. 2012
    ..The final recombinant P. ciferrii strain produced up to 199 mg((TAPS))g(-1)((cdw)) with a maximal production rate of 8.42 mg×OD(600nm)(-1)h(-1) and a titer of about 2 g L(-1), and should be applicable for industrial TAPS production...
  79. ncbi Neutrophil chemotaxis by pathogen-associated molecular patterns--formylated peptides are crucial but not the sole neutrophil attractants produced by Staphylococcus aureus
    Manuela C Dürr
    Medical Microbiology and Hygiene Department, University of Tubingen, Tubingen, Germany
    Cell Microbiol 8:207-17. 2006
    ..These data demonstrate an important role of FPs in innate immunity against bacterial infections and indicate that host chemotaxis receptors recognize a larger set of bacterial molecules than previously thought...
  80. pmc Identifying residue-residue clashes in protein hybrids by using a second-order mean-field approach
    Gregory L Moore
    Department of Chemical Engineering, Pennsylvania State University, 112 Fenske Laboratory, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 100:5091-6. 2003
    ....
  81. ncbi Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa
    B J McMorran
    Department of Biochemistry and Centre for Gene Research, University of Otago, PO Box 56, Dunedin, New Zealand
    Microbiology 147:1517-24. 2001
    ..These studies enhance understanding of the enzymology of pyoverdine synthesis, and to the best of the authors' knowledge provide the first example of involvement of a GART-type enzyme in synthesis of a secondary metabolite...
  82. ncbi Cloning and complete nucleotide sequence of Acinetobacter radioresistens CMC-1 AglyA gene encoding serine hydroxymethyltransferase
    M C Hong
    Department of Biochemistry, Medical College, National Cheng Kung University, Tainan, Taiwan
    FEMS Microbiol Lett 170:413-8. 1999
    ..The enzyme also showed a high level of homology to other sources of serine hydroxymethyltransferase proteins...
  83. ncbi Gene within a gene: nested Drosophila genes encode unrelated proteins on opposite DNA strands
    S Henikoff
    Cell 44:33-42. 1986
    ..Therefore, a housekeeping gene and a developmentally regulated gene function in a nested arrangement...
  84. ncbi Identification of genes involved in the sequestration of iron in mycobacteria: the ferric exochelin biosynthetic and uptake pathways
    E H Fiss
    Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 10461
    Mol Microbiol 14:557-69. 1994
    ..smegmatis mutant with the BCG cosmid restored the synthesis of the M. smegmatis exochelin, demonstrating the use of M. smegmatis as a surrogate host for analysis of exochelins from slow-growing mycobacteria...
  85. ncbi GART, SON, IFNAR, and CRF2-4 genes cluster on human chromosome 21 and mouse chromosome 16
    S Cheng
    Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206
    Mamm Genome 4:338-42. 1993
  86. ncbi Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase
    E A Stura
    Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Biol Chem 264:9703-6. 1989
    ..5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (less than 2.5 A) x-ray diffraction studies have been obtained for each of the above...
  87. ncbi On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2'-deoxycytidine 5'-monophosphate
    R Subramaniam
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143
    Arch Biochem Biophys 275:11-5. 1989
    ..We conclude that FdCMP is not a mechanism-based inhibitor of dCMP hydroxymethylase...
  88. pmc Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli
    P Nygaard
    Institute of Biological Chemistry B, University of Copenhagen, Denmark
    J Bacteriol 175:3591-7. 1993
    ..Under these conditions GAR accumulated. Addition of purine compounds or formate prevented growth inhibition. The regulation of the level of GAR transformylase T is controlled by the PurR protein and hypoxanthine...
  89. pmc Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene
    C Thylén
    Department of Microbiology, Faculty of Pharmacy, Uppsala University, Sweden
    J Bacteriol 170:1994-8. 1988
    ..A reading frame likely corresponding to gene 42 and putative promoter and terminator sequences were identified in the partial sequence of the cloned fragment...
  90. ncbi Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution
    C Klein
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 249:153-75. 1995
    ....
  91. pmc Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase: role of a 16-amino acid insertion module in initiator tRNA recognition
    V Ramesh
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 94:13524-9. 1997
    ..These and other considerations suggest that the 16-amino acid insertion in MTF plays an important role in the specific recognition of the determinants for formylation in the acceptor stem of the initiator tRNA...
  92. ncbi The primary structure of sheep liver cytosolic serine hydroxymethyltransferase and an analysis of the evolutionary relationships among serine hydroxymethyltransferases
    R Usha
    Department of Biochemistry, Indian Institute of Science, Bangalore
    Biochim Biophys Acta 1204:75-83. 1994
    ..However, the cysteines are not conserved among the various serine hydroxymethyltransferases. The exact role of the cysteines in the reaction catalyzed by serine hydroxymethyltransferase remains to be elucidated...
  93. ncbi Saccharomyces cerevisiae expresses two genes encoding isozymes of 5-aminoimidazole-4-carboxamide ribonucleotide transformylase
    A S Tibbetts
    Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA
    Arch Biochem Biophys 340:195-200. 1997
    ..Because it is likely that the proteins encoded by ADE16 and ADE17 also contain IMP cyclohydrolase activity, these two genes complete the set of clones and mutants for the entire de novo purine biosynthesis pathway in yeast...
  94. ncbi Isolation of human purH gene expressed in the rodent transformant cells by subtractive enrichment of 3'-untranslated region of human transcript
    M Yamauchi
    Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan
    DNA Res 2:269-75. 1995
    ..The full-length cDNA was isolated using the 3'-UTR clone as a probe, and its biological activity was confirmed by introducing it into the mouse purH-negative mutant cells...
  95. pmc Mapping the active site of the Haemophilus influenzae methionyl-tRNA formyltransferase: residues important for catalysis and tRNA binding
    D T Newton
    Guelph Waterloo Centre for Graduate Work in Chemistry and Biochemistry, Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Biochem J 339:63-9. 1999
    ..Together, these observations imply that this group of enzymes uses the same basic mechanism in formylating their substrates...
  96. ncbi Characterization of two 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase isozymes from Saccharomyces cerevisiae
    A S Tibbetts
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology and the Biochemical Institute, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 275:20920-7. 2000
    ..Ade16p was observed to be more abundant in cells grown on nonfermentable carbon sources than in glucose-grown cells, suggesting a role for this isozyme in respiration or sporulation...
  97. ncbi The amino-terminal region of the Escherichia coli T-protein of the glycine cleavage system is essential for proper association with H-protein
    K Okamura-Ikeda
    The Institute for Enzyme Research, University of Tokushima, Japan
    Eur J Biochem 264:446-53. 1999
    ..These results suggest that EH, when it interacts with ET, causes a change in conformation of ET and that the N-terminal region of ET is essential for the conformational change leading to the proper interaction with EH...
  98. pmc Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides
    S Lutz
    Department of Chemistry, The Pennsylvania State University, 414 Wartik Laboratory, University Park, PA 16802, USA
    Nucleic Acids Res 29:E16. 2001
    ..coli host strain. Multiple active hybrid enzymes were identified, including ones fused in regions of low sequence homology...
  99. pmc Striking effects of coupling mutations in the acceptor stem on recognition of tRNAs by Escherichia coli Met-tRNA synthetase and Met-tRNA transformylase
    C P Lee
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 89:9262-6. 1992
    ..G72 base pair at the end of an RNA helix. Thus, in some tRNAs, the discriminator base could affect stability of the base pair between nt 1 and 72 and thereby the structure of tRNA at the end of the acceptor stem...
  100. ncbi Recurrent mutations in P- and T-proteins of the glycine cleavage complex and a novel T-protein mutation (N145I): a strategy for the molecular investigation of patients with nonketotic hyperglycinemia (NKH)
    J R Toone
    Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada
    Mol Genet Metab 72:322-5. 2001
    ..In addition, a novel point mutation in T-protein, N145I, was found in a single case and a PCR/restriction enzyme assay was developed for its detection...
  101. ncbi Structure and chromosomal localization of the aminomethyltransferase gene (AMT)
    K Nanao
    Department of Pediatrics, Akita University School of Medicine, Japan
    Genomics 19:27-30. 1994
    ..Two putative glucocorticoid-responsive elements and a putative thyroid hormone-responsive element are present. The AMT gene was assigned to subband 3p21.2-p21.1 by fluorescence in situ hybridization...

Research Grants5

  1. MECHANISM & STRUCTURE OF HUMAN GAR TRANSFORMYLASE
    CAROL CAPERELLI; Fiscal Year: 2004
    ..Although it has been demonstrated that inhibition of this pathway shows potential for cancer chemotherapy, much basic information about the pathway and its constituent enzymes remains to be elucidated. ..
  2. Fostriecin and Related Protein Phosphatase Inhibitors
    Dale Boger; Fiscal Year: 2005
    ....
  3. FOLYLPOLYGLUTAMATE SYNTHETASE IN CANCER CHEMOTHERAPY
    John McGuire; Fiscal Year: 2003
    ..This may explain why high-level resistance to pulse MTX can occur in the absence of a large decrease in total FPGS activity or a rise in glutamyl hydrolase activity. ..
  4. Coordinated control of a regulatory network by gcvB RNA
    GEORGE STAUFFER; Fiscal Year: 2008
    ..Second, we will determine the scope of the regulatory network and the molecular mechanisms of control by the GcvB RNAs. [unreadable] [unreadable]..
  5. Enhancement of methotrexate uptake in childhood ALL
    John McGuire; Fiscal Year: 2009
    ..If successful, this research could provide a way to increase the effectiveness of methotrexate, a key drug used in treating childhood acute lymphoblastic leukemia, and thus increase the cure rate in this disease. ..