transaminases

Summary

Summary: A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.

Top Publications

  1. pmc Four of the most common mutations in primary hyperoxaluria type 1 unmask the cryptic mitochondrial targeting sequence of alanine:glyoxylate aminotransferase encoded by the polymorphic minor allele
    Sonia Fargue
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    J Biol Chem 288:2475-84. 2013
  2. ncbi Role of low native state kinetic stability and interaction of partially unfolded states with molecular chaperones in the mitochondrial protein mistargeting associated with primary hyperoxaluria
    Angel L Pey
    Departamento de Quimica Fisica, Facultad de Ciencias, Universidad de Granada, Spain
    Amino Acids 41:1233-45. 2011
  3. pmc Human wild-type alanine:glyoxylate aminotransferase and its naturally occurring G82E variant: functional properties and physiological implications
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochem J 408:39-50. 2007
  4. ncbi Primary hyperoxaluria Type 1: indications for screening and guidance for diagnosis and treatment
    Pierre Cochat
    Reference Center for Rare Renal Diseases and EPICIME, Department of Paediatrics, Hospices Civils de Lyon and Université Claude Bernard Lyon 1, Lyon, France
    Nephrol Dial Transplant 27:1729-36. 2012
  5. ncbi Process considerations for the asymmetric synthesis of chiral amines using transaminases
    Pär Tufvesson
    Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, DK 2800 Lyngby, Denmark
    Biotechnol Bioeng 108:1479-93. 2011
  6. pmc Rapid profiling of disease alleles using a tunable reporter of protein misfolding
    Adrianne M C Pittman
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Genetics 192:831-42. 2012
  7. ncbi Consequences of missense mutations for dimerization and turnover of alanine:glyoxylate aminotransferase: study of a spectrum of mutations
    M B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Children s and Women s Health Centre, Vancouver, BC, Canada
    Mol Genet Metab 89:349-59. 2006
  8. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
  9. ncbi Aminotransferases: demonstration of homology and division into evolutionary subgroups
    P K Mehta
    Biochemisches Institut der Universitat Zurich, Switzerland
    Eur J Biochem 214:549-61. 1993
  10. ncbi Primary hyperoxaluria type 1: update and additional mutation analysis of the AGXT gene
    Emma L Williams
    Clinical Biochemistry, University College London UCL Hospitals National Health Service NHS Trust, London, UK
    Hum Mutat 30:910-7. 2009

Detail Information

Publications275 found, 100 shown here

  1. pmc Four of the most common mutations in primary hyperoxaluria type 1 unmask the cryptic mitochondrial targeting sequence of alanine:glyoxylate aminotransferase encoded by the polymorphic minor allele
    Sonia Fargue
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    J Biol Chem 288:2475-84. 2013
    ....
  2. ncbi Role of low native state kinetic stability and interaction of partially unfolded states with molecular chaperones in the mitochondrial protein mistargeting associated with primary hyperoxaluria
    Angel L Pey
    Departamento de Quimica Fisica, Facultad de Ciencias, Universidad de Granada, Spain
    Amino Acids 41:1233-45. 2011
    ....
  3. pmc Human wild-type alanine:glyoxylate aminotransferase and its naturally occurring G82E variant: functional properties and physiological implications
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochem J 408:39-50. 2007
    ..Overall, careful biochemical analyses have allowed elucidation of the mechanism of action of AGT and the way in which the disease causing G82E mutation affects it...
  4. ncbi Primary hyperoxaluria Type 1: indications for screening and guidance for diagnosis and treatment
    Pierre Cochat
    Reference Center for Rare Renal Diseases and EPICIME, Department of Paediatrics, Hospices Civils de Lyon and Université Claude Bernard Lyon 1, Lyon, France
    Nephrol Dial Transplant 27:1729-36. 2012
    ..In chronic kidney disease Stages 4 and 5, the best outcomes to date were achieved with combined liver-kidney transplantation...
  5. ncbi Process considerations for the asymmetric synthesis of chiral amines using transaminases
    Pär Tufvesson
    Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, DK 2800 Lyngby, Denmark
    Biotechnol Bioeng 108:1479-93. 2011
    ..This article also points to the interaction between the solutions and the need for a process development strategy based on fundamental principles...
  6. pmc Rapid profiling of disease alleles using a tunable reporter of protein misfolding
    Adrianne M C Pittman
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Genetics 192:831-42. 2012
    ....
  7. ncbi Consequences of missense mutations for dimerization and turnover of alanine:glyoxylate aminotransferase: study of a spectrum of mutations
    M B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Children s and Women s Health Centre, Vancouver, BC, Canada
    Mol Genet Metab 89:349-59. 2006
    ..The minor allele AGT product and many of the mutants were subject to a limited non-proteasomal proteolytic cleavage when ATP was depleted...
  8. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
    ..1 M urea. These biochemical results are discussed in the light of the characteristics of the enzymatic phenotype of PH1 patients bearing G170R mutation in AGT-Mi and the positive response of these patients to pyridoxine treatment...
  9. ncbi Aminotransferases: demonstration of homology and division into evolutionary subgroups
    P K Mehta
    Biochemisches Institut der Universitat Zurich, Switzerland
    Eur J Biochem 214:549-61. 1993
    ..Apparently, the aminotransferases constitute a group of homologous proteins which diverged into subgroups and, with some exceptions, into substrate-specific individual enzymes already in the universal ancestor cell...
  10. ncbi Primary hyperoxaluria type 1: update and additional mutation analysis of the AGXT gene
    Emma L Williams
    Clinical Biochemistry, University College London UCL Hospitals National Health Service NHS Trust, London, UK
    Hum Mutat 30:910-7. 2009
    ..This review discusses the spectrum of AGXT mutations and polymorphisms, their clinical significance, and their diagnostic relevance...
  11. pmc Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis
    Joachim Schuster
    Molekulare Botanik, Universitat Ulm, 89069 Ulm, Germany
    Plant Cell 18:2664-79. 2006
    ..This suggests that the two pathways have a common evolutionary origin...
  12. ncbi Crystal structure of alanine:glyoxylate aminotransferase and the relationship between genotype and enzymatic phenotype in primary hyperoxaluria type 1
    Xiaoxuan Zhang
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 331:643-52. 2003
    ....
  13. pmc Auxin biosynthesis: a simple two-step pathway converts tryptophan to indole-3-acetic acid in plants
    Yunde Zhao
    Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0116, USA
    Mol Plant 5:334-8. 2012
    ..The two-step conversion of Trp to IAA is the main auxin biosynthesis pathway that plays an essential role in many developmental processes...
  14. pmc A novel, simple, and sensitive colorimetric method to determine aromatic amino acid aminotransferase activity using the Salkowski reagent
    Michał Szkop
    Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, SGGW, Poland
    Folia Microbiol (Praha) 57:1-4. 2012
    ..For comparative purposes, ArAT activity was also determined by high-performance liquid chromatography. ArAT activity staining in polyacrylamide gels with the Salkowski reagent is also presented...
  15. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: characterization of the two allelic forms and their pathogenic variants
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochim Biophys Acta 1814:1577-84. 2011
    ..This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology...
  16. ncbi Primary hyperoxalurias: disorders of glyoxylate detoxification
    Eduardo Salido
    Hospital Universitario de Canarias, Universidad La Laguna, Tenerife, Spain
    Biochim Biophys Acta 1822:1453-64. 2012
    ..It is reasonable to expect that recent advances in the understanding of the molecular mechanisms of disease will result into better targeted therapeutic options in the future...
  17. ncbi Rational assignment of key motifs for function guides in silico enzyme identification
    Matthias Höhne
    Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany
    Nat Chem Biol 6:807-13. 2010
    ..Using this in silico approach, we have discovered 17 (R)-selective amine transaminases, which catalyzed the synthesis of several (R)-amines with excellent optical purity up to >99% enantiomeric ..
  18. ncbi The effect of loss of excess weight on the metabolic risk factors after bariatric surgery in morbidly and super-obese patients
    Anna Maria Wolf
    Department for General, Visceral and Transplantation Surgery, University Hospital, Ulm, Germany
    Obes Surg 17:910-9. 2007
    ....
  19. pmc Divergent roles in Arabidopsis thaliana development and defense of two homologous genes, aberrant growth and death2 and AGD2-LIKE DEFENSE RESPONSE PROTEIN1, encoding novel aminotransferases
    Jong Tae Song
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637
    Plant Cell 16:353-66. 2004
    ..We suggest that AGD2 synthesizes an important amino acid-derived molecule that promotes development and suppresses defenses, whereas ALD1 generates a related amino acid-derived molecule important for activating defense signaling...
  20. ncbi Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence
    Mark Wogulis
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Biochemistry 47:1608-21. 2008
    ..Biochemical, bioinformatics, genetic and in vivo metabolite data derived from LC-MS demonstrate that YDR428C, which we have designated BNA7, is the yeast FKF...
  21. ncbi Zinc supplementation prevents the increase of transaminase in chronic hepatitis C patients during combination therapy with pegylated interferon alpha-2b and ribavirin
    Yasuko Murakami
    Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama 719 1197, Japan
    J Nutr Sci Vitaminol (Tokyo) 53:213-8. 2007
    ..These observations indicate that polaprezinc supplementation may have induced some antioxidative functions in the liver which resulted in reduced hepatocyte injury during PEG-IFN alpha-2b plus ribavirin therapy...
  22. pmc Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1
    Krisztian Fodor
    European Molecular Biology Laboratory Hamburg, Hamburg, Germany
    PLoS Biol 10:e1001309. 2012
    ..We discuss how the knowledge of the molecular parameters for alanine-glyoxylate aminotransferase required for peroxisomal translocation could become useful for improved hyperoxaluria type 1 treatment...
  23. ncbi Crystal structures of Paracoccus denitrificans aromatic amino acid aminotransferase: a substrate recognition site constructed by rearrangement of hydrogen bond network
    A Okamoto
    Department of Biochemistry, Osaka Medical College, Takatsuki, Osaka, 569 8686, Japan
    J Mol Biol 280:443-61. 1998
    ..Consequently, the rearrangement of the hydrogen bond network can form recognition sites for both acidic and aromatic side-chains of the substrate without a conformational change in the backbone structure in pdAroAT...
  24. ncbi Selected exonic sequencing of the AGXT gene provides a genetic diagnosis in 50% of patients with primary hyperoxaluria type 1
    Emma Williams
    Clinical Biochemistry, University College London Hospitals, London, United Kingdom
    Clin Chem 53:1216-21. 2007
    ..5% of PH1 patients, eliminating the need for a liver biopsy. Having reviewed the distribution of all AGXT mutations, we have evaluated a diagnostic strategy that uses selected exon sequencing for the molecular diagnosis of PH1...
  25. ncbi Overexpression of human alanine:glyoxylate aminotransferase in Escherichia coli: renaturation from guanidine-HCl and affinity for pyridoxal phosphate co-factor
    Marion B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada V6H 3N1
    Protein Expr Purif 41:18-26. 2005
    ..Furthermore, our data show that mutations with serious consequences in vivo may not be inherently catalytically inactive and may be rescuable...
  26. ncbi Lifestyle intervention and antioxidant therapy in children with nonalcoholic fatty liver disease: a randomized, controlled trial
    Valerio Nobili
    Liver Unit, Bambino Gesu Children s Hospital and Research Institute, Rome, Italy
    Hepatology 48:119-28. 2008
    ..The improvement in all these parameters was not significantly different between the two groups...
  27. ncbi Performance of noninvasive markers for liver fibrosis is reduced in chronic hepatitis C with normal transaminases
    G Sebastiani
    Venetian Institute of Molecular Medicine, Padova, Italy
    J Viral Hepat 15:212-8. 2008
    ..Liver biopsy may still be needed for many of these cases to correctly stage liver fibrosis. Specific noninvasive tools and possibly combination of markers should be developed and validated in this clinical setting...
  28. pmc Primary hyperoxaluria type 1 in the Canary Islands: a conformational disease due to I244T mutation in the P11L-containing alanine:glyoxylate aminotransferase
    A Santana
    Department of Pediatrics, University of California School of Medicine, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 100:7277-82. 2003
    ..In summary, I244T, the second most common mutation responsible for PH1, is a protein conformational disease that may benefit from new therapies with pharmacological chaperones or small molecules to minimize protein aggregation...
  29. pmc Molecular defects of the glycine 41 variants of alanine glyoxylate aminotransferase associated with primary hyperoxaluria type I
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Proc Natl Acad Sci U S A 107:2896-901. 2010
    ..The detailed insight into how G41 mutations act on the structure-function of AGT may contribute to achieve the ultimate goal of correcting the effects of these mutations...
  30. ncbi Primary hyperoxaluria type 1: AGT mistargeting highlights the fundamental differences between the peroxisomal and mitochondrial protein import pathways
    Christopher J Danpure
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1763:1776-84. 2006
    ....
  31. pmc Molecular Insight into the Synergism between the Minor Allele of Human Liver Peroxisomal Alanine:Glyoxylate Aminotransferase and the F152I Mutation
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    J Biol Chem 284:8349-58. 2009
    ....
  32. ncbi Clinical implications of mutation analysis in primary hyperoxaluria type 1
    Christiaan S van Woerden
    Emma Children s Hospital AMC, Amsterdam, The Netherlands
    Kidney Int 66:746-52. 2004
    ..The aim of this study was to determine this association in order to find clues for improvement of patient care...
  33. ncbi Weight loss and non-alcoholic fatty liver disease: falls in gamma-glutamyl transferase concentrations are associated with histologic improvement
    John B Dixon
    Centre for Obesity Research and Education, Monash University, Alfred Hospital, Melbourne, Australia
    Obes Surg 16:1278-86. 2006
    ..The ability for aminotransferase levels to track histological features of non-alcoholic fatty liver disease (NAFLD) with weight loss has not been examined...
  34. ncbi Pyridoxine effect in type I primary hyperoxaluria is associated with the most common mutant allele
    Carla G Monico
    Mayo Clinic College of Medicine, Mayo Clinic Foundation, Rochester, Minnesota 55905, USA
    Kidney Int 67:1704-9. 2005
    ..Mechanisms of VB6 action on hepatic alanine:glyoxylate aminotransferase (AGT), the deficient enzyme in PHI, and VB6 dose response have not been well-characterized...
  35. ncbi Peroxisomal alanine:glyoxylate aminotransferase deficiency in primary hyperoxaluria type I
    C J Danpure
    FEBS Lett 201:20-4. 1986
    ..This enzyme deficiency explains most of the biochemical characteristics of the disease and means that primary hyperoxaluria type I should be added to the rather select list of peroxisomal disorders...
  36. ncbi Functional synergism between the most common polymorphism in human alanine:glyoxylate aminotransferase and four of the most common disease-causing mutations
    M J Lumb
    Medical Research Council Laboratory for Molecular Cell Biology and the Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 275:36415-22. 2000
    ..These results are discussed in the light of the high allelic frequency ( approximately 20%) of the P11L polymorphism and its importance in determining the phenotypic manifestations of mutations in PH1...
  37. pmc Alanine-glyoxylate aminotransferase-deficient mice, a model for primary hyperoxaluria that responds to adenoviral gene transfer
    Eduardo C Salido
    Unidad Investigación, Hospital Universitario Canarias, University La Laguna, Tenerife 38320, Spain
    Proc Natl Acad Sci U S A 103:18249-54. 2006
    ....
  38. ncbi Rapid and sensitive kinetic assay for characterization of omega-transaminases
    Sebastian Schätzle
    Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, Greifswald University, Felix Hausdorff Str 4, 17487 Greifswald, Germany
    Anal Chem 81:8244-8. 2009
    For the biocatalytic preparation of optically active amines, omega-transaminases (omega-TA) are of special interest since they allow the asymmetric synthesis starting from prostereogenic ketones with 100% yield...
  39. pmc In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase
    Erin D Hopper
    Department of Biology and Chemistry, Duke University, Durham, North Carolina 27708, USA
    J Biol Chem 283:30493-502. 2008
    ..e. pharmacological chaperones) may be effective for treatment of primary hyperoxaluria, we propose that the methods described here can be used in high throughput screens for compounds that stabilize AGT mutants...
  40. ncbi Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17
    J S Shin
    Bioengineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA
    Appl Microbiol Biotechnol 61:463-71. 2003
    ..The deduced amino acid sequence (453 residues) showed significant homology with omega-amino acid:pyruvate transaminases (omega-APT) from various bacterial strains (80 identical residues with four omega-APTs)...
  41. pmc Structure of GroEL in complex with an early folding intermediate of alanine glyoxylate aminotransferase
    Armando Albert
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Madrid E 28006, Spain
    J Biol Chem 285:6371-6. 2010
    ....
  42. ncbi A novel transaminase, (R)-amine:pyruvate aminotransferase, from Arthrobacter sp. KNK168 (FERM BP-5228): purification, characterization, and gene cloning
    Akira Iwasaki
    QOL Division Technology Management Department Research Group, Kaneka Corporation, Takasago, Hyogo, Japan
    Appl Microbiol Biotechnol 93:1563-73. 2012
    ..The deduced amino acid sequence was found to be homologous to those of the aminotransferases belonging to fold class IV of pyridoxal-5'-phosphate-dependent enzymes, such as branched-chain amino acid aminotransferases...
  43. ncbi Crystal structures of the Chromobacterium violaceumω-transaminase reveal major structural rearrangements upon binding of coenzyme PLP
    Maria S Humble
    Division of Biochemistry, KTH Royal Institute of Technology, Stockholm, Sweden
    FEBS J 279:779-92. 2012
    ..Comparison with other known transaminase crystal structures suggests that ordering of the 'roof' structure may be associated with substrate binding in Cv-ωTA and some other transaminases.
  44. pmc Biochemical analyses are instrumental in identifying the impact of mutations on holo and/or apo-forms and on the region(s) of alanine:glyoxylate aminotransferase variants associated with primary hyperoxaluria type I
    Elisa Oppici
    Department of Life Sciences and Reproduction, Section of Biological Chemistry, University of Verona, Strada Le Grazie 8 37134 Verona, Italy
    Mol Genet Metab 105:132-40. 2012
    ..e. active site microenvironment, large and/or small domain) of the protein are affected by each mutation, and (iii) suggest the possible therapeutic approach for patients bearing the examined mutations...
  45. ncbi Features and technical applications of ω-transaminases
    M Shaheer Malik
    Department of Biotechnology, Yonsei University, Seoul, South Korea
    Appl Microbiol Biotechnol 94:1163-71. 2012
    ..We also address crucial factors to be considered for the development of efficient ω-TA processes...
  46. pmc The Escherichia coli starvation gene cstC is involved in amino acid catabolism
    C D Fraley
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Bacteriol 180:4287-90. 1998
    ..Both sigmaS and sigma54 promoters are probably involved in cstC transcription and appear to compete with each other, presumably to match cstC expression to the cellular amino acid catabolic needs...
  47. ncbi Involvement of branched-chain amino acid aminotransferase (Bcat1/Eca39) in apoptosis
    A Eden
    Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel
    FEBS Lett 457:255-61. 1999
    ..This observation suggests that the growth inhibitory effect of BCAT1/ECA39 and its apoptosis promoting effect may be mediated by the levels of the products of BCAT1/ECA39 activity, namely, branched-chain keto acids...
  48. pmc ECA39, a conserved gene regulated by c-Myc in mice, is involved in G1/S cell cycle regulation in yeast
    O Schuldiner
    Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Israel
    Proc Natl Acad Sci U S A 93:7143-8. 1996
    ..Moreover, mutant strains show higher rates of UV-induced mutations. The results suggest that the product of ECA39 is involved in the regulation of G1 to S transition...
  49. ncbi Increased liver echogenicity at ultrasound examination reflects degree of steatosis but not of fibrosis in asymptomatic patients with mild/moderate abnormalities of liver transaminases
    U L Mathiesen
    Department of Internal Medicine, County Hospital, Oskarshamn, Sweden
    Dig Liver Dis 34:516-22. 2002
    ..To investigate whether hyperechogenicity of liver can reliably be interpreted as liver steatosis and if any concomitant or isolated fibrosis can be disclosed...
  50. ncbi Molecular basis for the dual mitochondrial and cytosolic localization of alanine:glyoxylate aminotransferase in amphibian liver cells
    Joanna D Holbrook
    Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 277:2336-44. 2002
    ..These results are discussed not only in terms of the molecular evolution of AGT targeting but also in terms of the ancillary requirements for the peroxisomal targeting of human AGT...
  51. pmc The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins
    Ruth Diebold
    Molekulare Botanik, Universitat Ulm, Albert Einstein Allee 11, 89069 Ulm, Germany
    Plant Physiol 129:540-50. 2002
    Branched-chain amino acid transaminases (BCATs) play a crucial role in the metabolism of leucine, isoleucine, and valine...
  52. ncbi Functional evidence for a nasopharyngeal carcinoma-related gene BCAT1 located at 12p12
    Wen Zhou
    Cancer Research Institute, Xiang Ya School of Medicine, Central South University, Hunan 410078, PR China
    Oncol Res 16:405-13. 2007
    ..As expected, the depletion of BCAT1 could effectively block the proliferation of NPC cells. The BCAT1 identified in the amplified 12p11-p12 region may play a certain role in NPC development...
  53. ncbi Construction, purification and characterization of untagged human liver alanine-glyoxylate aminotransferase expressed in Escherichia coli
    Barbara Cellini
    Dipartimento di Scienze Morfologico Biomediche, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Protein Pept Lett 15:153-9. 2008
    ..This is prerequisite to understand how pathological mutations affect the proper native quaternary and tertiary structure, stability, and targeting of the enzyme...
  54. ncbi A glycine-to-glutamate substitution abolishes alanine:glyoxylate aminotransferase catalytic activity in a subset of patients with primary hyperoxaluria type 1
    P E Purdue
    Biochemical Genetics Research Group, Clinical Research Centre, Harrow, Middlesex, United Kingdom
    Genomics 13:215-8. 1992
    ..However, the protein sequence in this region is highly conserved between different mammals, and the substitution at residue 82 is predicted to cause significant local structural alterations...
  55. ncbi Molecular aetiology of primary hyperoxaluria and its implications for clinical management
    Christopher J Danpure
    Department of Biology, University College London, Gower Street, London, WC1E 6BT, UK
    Expert Rev Mol Med 6:1-16. 2004
    ..New treatments that aim to counter the effects of specific mutations on the properties of the enzymes could be feasible in the not-too-distant future...
  56. ncbi AGXT gene mutations and their influence on clinical heterogeneity of type 1 primary hyperoxaluria
    A Amoroso
    Section of Genetics, Department of Reproductive and Developmental Science, University of Trieste, Via dell Istria 65 1 34137 Trieste, Italy
    J Am Soc Nephrol 12:2072-9. 2001
    ..05). G630A mutation homozygotes had a higher AGT residual activity (P = 0.00001). This study confirms the allelic heterogeneity of the AGXT, which could to some extent be responsible for the phenotypic heterogeneity in PH1...
  57. pmc Normal serum aminotransferase concentration and risk of mortality from liver diseases: prospective cohort study
    Hyeon Chang Kim
    Department of Preventive Medicine and Public Health, Yonsei University College of Medicine, 134 Shinchon dong, Seodaemun gu, Seoul 120 752, Republic of Korea
    BMJ 328:983. 2004
    ..To examine the relation between the normal range of serum aminotransferase concentration and mortality from liver disease...
  58. ncbi Characterization and chromosomal mapping of a genomic clone encoding human alanine:glyoxylate aminotransferase
    P E Purdue
    Biochemical Genetics Research Group, Clinical Research Centre, Harrow, Middlesex, United Kingdom
    Genomics 10:34-42. 1991
    ..The gene symbol AGXT has been assigned for this locus...
  59. pmc Mistargeting of peroxisomal L-alanine:glyoxylate aminotransferase to mitochondria in primary hyperoxaluria patients depends upon activation of a cryptic mitochondrial targeting sequence by a point mutation
    P E Purdue
    Biochemical Genetics Research Group, Clinical Research Centre, Harrow, Middlesex, United Kingdom
    Proc Natl Acad Sci U S A 88:10900-4. 1991
    ..These results are discussed with reference to the AGT targeting defect in primary hyperoxaluria and also in relation to the highly unusual species specificity of subcellular distribution of AGT among mammals...
  60. pmc L,L-diaminopimelate aminotransferase, a trans-kingdom enzyme shared by Chlamydia and plants for synthesis of diaminopimelate/lysine
    Andrea J McCoy
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 103:17909-14. 2006
    ....
  61. ncbi Virus-induced silencing of Comt, pAmt and Kas genes results in a reduction of capsaicinoid accumulation in chili pepper fruits
    Ma del Rosario Abraham-Juárez
    Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del IPN Cinvestav Campus Guanajuato, Km 9 6 Libramiento Norte Carretera Irapuato León, Apartado Postal 629, 36500, Irapuato, Gto, Mexico
    Planta 227:681-95. 2008
    ..Using this approach it was possible to generate non-pungent chili peppers at high efficiency...
  62. ncbi Synthesis and turnover of folates in plants
    Andrew D Hanson
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
    Curr Opin Plant Biol 5:244-9. 2002
    ..Moreover, new analytical advances will help to elucidate plant folate turnover and transport, which are practically unexplored...
  63. pmc Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli
    B L Schneider
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75083 0688, USA
    J Bacteriol 180:4278-86. 1998
    ..We conclude that the AST pathway is necessary for aerobic arginine catabolism in E. coli and that at least one enzyme of this pathway contributes to ornithine catabolism...
  64. ncbi Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels
    Ravishankar Palanivelu
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Cell 114:47-59. 2003
    ..Interestingly, wild-type tubes exhibit normal growth and guidance in pop2 pistils, perhaps by degrading excess GABA and sharpening the gradient leading to the micropyle...
  65. pmc Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase
    ANDRE O HUDSON
    Biotech Center and Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey 08901, USA
    J Bacteriol 190:3256-63. 2008
    ....
  66. pmc Nonalcoholic fatty liver disease
    Leon A Adams
    Division of Gastroenterology and Hepatology, Mayo Clinic and College of Medicine, Rochester, Minn 55905, USA
    CMAJ 172:899-905. 2005
    ..In this article we discuss the etiology, pathogenesis and diagnosis of nonalcoholic fatty liver disease as well as approaches to its management...
  67. ncbi Partial trypsin digestion as an indicator of mis-folding of mutant alanine:glyoxylate aminotransferase and chaperone effects of specific ligands. Study of a spectrum of missense mutants
    M B Coulter-Mackie
    Department of Pediatrics, Children and Women s Health Centre of BC, University of British Columbia, 4500 Oak Street, Vancouver, BC, Canada
    Mol Genet Metab 94:368-74. 2008
    ..For selected mutations the sensitivity to trypsin could be ameliorated by addition of pyridoxal phosphate or aminooxy acetic acid as specific pharmacological chaperones...
  68. pmc Correction of an enzyme trafficking defect in hereditary kidney stone disease in vitro
    Michael J Lumb
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochem J 374:79-87. 2003
    ..Results are discussed in the light of the known structural requirements of the two protein trafficking pathways and the formulation of possible treatment strategies for primary hyperoxaluria type 1...
  69. pmc An LL-diaminopimelate aminotransferase defines a novel variant of the lysine biosynthesis pathway in plants
    ANDRE O HUDSON
    Biotech Center and Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey 08901, USA
    Plant Physiol 140:292-301. 2006
    ..These results demonstrate that the Lys biosynthesis pathway in plants and cyanobacteria is distinct from the pathways that have so far been defined in microorganisms...
  70. ncbi Alanine : glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance
    Thomas Schlosser
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, 52425 Julich, Germany
    Yeast 21:63-73. 2004
    ..This means that growth is not possible on glucose but on ethanol as the sole carbon source. Additional disruption of AGX1 revealed a complete glycine auxotrophy. Complementation was observed by transformation with a plasmid-encoded AGX1...
  71. ncbi Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine: induced fit and substrate recognition of the enzyme
    K Okada
    Department of Chemistry, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    Biochemistry 40:7453-63. 2001
    ....
  72. pmc Enzymic imbalance in serine metabolism in human colon carcinoma and rat sarcoma
    K Snell
    Laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis 46223
    Br J Cancer 57:87-90. 1988
    ....
  73. pmc ECA39 is a novel distant metastasis-related biomarker in colorectal cancer
    Reigetsu Yoshikawa
    Second Department of Surgery, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo 663 8501, Japan
    World J Gastroenterol 12:5884-9. 2006
    ..To investigate the possible role of polysaccharide-K (PSK) -related markers in predicting distant metastasis and in the clinical outcome of colorectal cancer (CRC)...
  74. pmc Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
    Yuchen Liu
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    J Bacteriol 192:3304-10. 2010
    ..These results demonstrated that the DapL pathway is present in marine methanogens belonging to the Methanococcales...
  75. ncbi omega-Transaminases for the synthesis of non-racemic alpha-chiral primary amines
    Dominik Koszelewski
    Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A 8010 Graz, Austria
    Trends Biotechnol 28:324-32. 2010
    ..omega-Transaminases (TAs) can be applied either for the kinetic resolution of racemic amines or for the asymmetric synthesis of ..
  76. pmc Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of
    A Motley
    Department of Biochemistry, E C Slater Institute, University of Amsterdam, The Netherlands
    J Cell Biol 131:95-109. 1995
    ....
  77. pmc Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa
    Y Itoh
    Division of Applied Microbiology, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, Japan
    J Bacteriol 179:7280-90. 1997
    ..2 to 39.5 min (Kohara clone 327; GenBank/EMBL/DDJB accession no. D90818). The overall organization of the aru and ast genes in both organisms is similar, with the exception that E. coli appears to have a single AST gene...
  78. pmc Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1
    P E Purdue
    Biochemical Genetics Research Group, Clinical Research Centre, Harrow, Middlesex, United Kingdom
    J Cell Biol 111:2341-51. 1990
    ....
  79. ncbi Characterization of murine BCAT genes: Bcat1, a c-Myc target, and its homolog, Bcat2
    T Ben-Yosef
    Department of Genetics, Hebrew University of Jerusalem, Israel
    Mamm Genome 9:595-7. 1998
  80. ncbi Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases
    A Eden
    Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    J Biol Chem 271:20242-5. 1996
    ..We suggest that BCAT is a target for c-Myc activity and discuss the evolutionary conservation of prokaryotic and eukaryotic BCATs and their possible involvement in regulation of cell proliferation...
  81. ncbi Molecular etiology of primary hyperoxaluria type 1: new directions for treatment
    Christopher J Danpure
    Department of Biology, University College London, London, UK
    Am J Nephrol 25:303-10. 2005
    ....
  82. pmc Dynamics of HEV viremia, fecal shedding and its relationship with transaminases and antibody response in patients with sporadic acute hepatitis E
    Nidhi S Chandra
    Department of Gastroenterology, SMS Medical College and Hospital, Jaipur Rajasthan, India
    Virol J 7:213. 2010
    ..of fecal excretion and viremia on sequential samples from individual patients and its correlation with serum transaminases and antibody responses in patients with acute hepatitis E...
  83. ncbi Evaluation of mutation screening as a first line test for the diagnosis of the primary hyperoxalurias
    Gill Rumsby
    Clinical Biochemistry, UCL Hospitals, London, United Kingdom
    Kidney Int 66:959-63. 2004
    ..We have evaluated a molecular genetic approach for the diagnosis of these autosomal-recessive diseases...
  84. ncbi Crystal structure of LL-diaminopimelate aminotransferase from Arabidopsis thaliana: a recently discovered enzyme in the biosynthesis of L-lysine by plants and Chlamydia
    Nobuhiko Watanabe
    Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    J Mol Biol 371:685-702. 2007
    ..A malate-bound structure of AtDAP-AT allowed LL-DAP and L-glutamate to be modelled into the active site. These initial three-dimensional structures of LL-DAP-AT provide insight into its substrate specificity and catalytic mechanism...
  85. ncbi Aromatic amino acid aminotransferase activity and indole-3-acetic acid production by associative nitrogen-fixing bacteria
    Raúl Osvaldo Pedraza
    Centro de investigaciones microbiológicas, Instituto de Ciencias, Universidad Autonoma de Puebla, Apdo Postal 1622 C P 72, 000, Puebla Pue, Mexico
    FEMS Microbiol Lett 233:15-21. 2004
    ..9 microg/mg protein in culture medium supplemented with tryptophan. The IAA production may enable bacteria promote a growth-promoting effect in plants, in addition to their nitrogen fixing ability...
  86. ncbi Cloning and nucleotide sequence of cDNA encoding human liver serine-pyruvate aminotransferase
    K Nishiyama
    Department of Biochemistry, Hamamatsu University School of Medicine, Shizuoka, Japan
    Eur J Biochem 194:9-18. 1990
    ....
  87. ncbi Molecular determinants for substrate selectivity of ω-transaminases
    Eul Soo Park
    Department of Biotechnology, Yonsei University, Seodaemun gu, Seoul, South Korea
    Appl Microbiol Biotechnol 93:2425-35. 2012
    ..The six key residues were found to be essentially conserved among nine ω-TA sequences, underlying the molecular basis for the high similarity in the substrate selectivity...
  88. ncbi Alcohol-induced free radicals in mice: direct toxicants or signaling molecules?
    M Yin
    Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Hepatology 34:935-42. 2001
    ..These findings are consistent with the hypothesis that free radicals act as redox signals for TNF-alpha production and do not directly damage cells in early alcohol-induced hepatic injury...
  89. pmc Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763
    M Yvon
    Unité de Recherche de Biochimie et Structure des Protéines, I N R A, 78352 Jouy en Josas, France
    Appl Environ Microbiol 66:571-7. 2000
    ..Since this enzyme is still active under cheese-ripening conditions, it certainly plays a major role in cheese flavor development...
  90. ncbi Curcumin protects against concanavalin A-induced hepatitis in mice through inhibiting the cytoplasmic translocation and expression of high mobility group box 1
    Chuan Tao Tu
    Department of Gastroenterology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China
    Inflammation 36:206-15. 2013
    ....
  91. ncbi Expression and functional analysis of aminotransferase involved in indole-3-acetic acid biosynthesis in Azospirillum brasilense Yu62
    Shi Mei Ge
    Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100094, PR China
    Biochemistry (Mosc) 74:81-4. 2009
    ..Thus, we deduced that AtrC protein is identical to the 44 kD band of crude protein extracts. The optimal temperature and pH for the catalytic activity of the purified AtrC are 30 degrees C and pH 7.0, respectively...
  92. ncbi The interleukin 28B rs12979860 C/T polymorphism and serum cholesterol as predictors of fibrosis progression in patients with chronic hepatitis C and persistently normal transaminases
    Carlo Fabris
    Department of Experimental and Clinical Medical Sciences, Medical Liver Transplantation Unit, Internal Medicine, University of Udine, Udine, Italy
    J Med Virol 84:747-55. 2012
    ..To clarify this issue, 93 untreated patients infected with HCV with normal or near-normal transaminases and an initial Ishak staging score ≤1 were investigated retrospectively in the longitudinal study (median ..
  93. ncbi Conductometric method for the rapid characterization of the substrate specificity of amine-transaminases
    Sebastian Schätzle
    Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, Greifswald University, Felix Hausdorff Strasse 4, 17487 Greifswald, Germany
    Anal Chem 82:2082-6. 2010
    Amine-transaminases (ATAs, omega-transaminases, omega-TA) are PLP-dependent enzymes that catalyze amino group transfer reactions...
  94. ncbi A serine-to-phenylalanine substitution leads to loss of alanine:glyoxylate aminotransferase catalytic activity and immunoreactivity in a patient with primary hyperoxaluria type 1
    Y Minatogawa
    Department of Biochemistry, Wakayama Medical College, Japan
    Hum Mol Genet 1:643-4. 1992
  95. pmc The peroxisomal targeting sequence type 1 receptor, Pex5p, and the peroxisomal import efficiency of alanine:glyoxylate aminotransferase
    T G Knott
    MRC Laboratory for Molecular Cell Biology and Department of Biology, University College London, Gower Street, London WC1E 6BT, U K
    Biochem J 352:409-18. 2000
    ..They also suggest that the non-consensus PTS1 of human AGT might interact with HsPex5p very differently compared with the consensus PTS1, Ser-Lys-Leu...
  96. ncbi Functional analysis of the 5'-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT
    Manabu Sato
    Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama, Japan
    Biochim Biophys Acta 1574:205-9. 2002
    ..It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements...
  97. ncbi The major allele of the alanine:glyoxylate aminotransferase gene: nine novel mutations and polymorphisms associated with primary hyperoxaluria type 1
    Marion B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
    Mol Genet Metab 86:172-8. 2005
    ..S218L was apparently homozygous in two individuals. These findings contribute to our overall picture of heterogeneity of mutations in PH1 and the AGT major allele...
  98. ncbi Is liver transaminases assessment an appropriate tool for the screening of non-alcoholic fatty liver disease in at risk obese children and adolescents?
    G Rodriguez
    Growth, Exercise, NUtrition and Development Research Group, Departamento de Pediatria, Radiologia y Medicina Fisica, University of Zaragoza, and Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain
    Nutr Hosp 25:712-7. 2010
    ..To detect NAFLD in obese children and adolescents, transaminases serum concentrations should be routinely determined in these patients...
  99. pmc Structural insight into the mechanism of substrate specificity of aedes kynurenine aminotransferase
    Qian Han
    Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, USA
    Biochemistry 47:1622-30. 2008
    ....
  100. ncbi Evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. A survey of its subcellular distribution in the livers of various representatives of the classes Mammalia, Aves and Amphibia
    C J Danpure
    Biochemical Genetics Research Group, MRC Clinical Research Centre, Harrow, United Kingdom
    Eur J Cell Biol 64:295-313. 1994
    ....
  101. ncbi Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology
    Malgorzata Rak
    Institut de Biochimie et Génétique Cellulaires, Universite Victor Segalen, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    J Biol Chem 282:10853-64. 2007
    ..However, the atp6 mutant exhibited unusual mitochondrial structure and distribution anomalies, presumably caused by a strong delay in inner membrane fusion...

Research Grants62

  1. LIVER PRESERVATION FOR TRANSPLANTATION
    John J Lemasters; Fiscal Year: 2012
    ..b>Transaminases increase well beyond the upper limit of normal in virtually every human liver transplantation, indicating ..
  2. Clinical and genomic responses to remote ischemic preconditioning
    Brian W McCrindle; Fiscal Year: 2010
    ..pulmonary (pulmonary and airway mechanics, ventilation parameters), hematologic (blood counts), hepatic (transaminases, INR), metabolic (lactate, arterial and mixed venous oxygen saturations) dysfunction and inflammatory markers (..
  3. Discovering tissue-specific biomarkers of radiation injury in SILAC-labeled mice
    Amanda G Paulovich; Fiscal Year: 2013
    ..Precedents include the troponins in myocardial infarction, transaminases in liver injury, creatine kinase in muscle injury, and lipase in pancreatic processes...
  4. Ursodeoxycholic Acid Therapy in Pediatric Primary Sclerosing CholangitisA pilot
    Benjamin L Shneider; Fiscal Year: 2012
    ..including a stronger association with both autoimmune markers and histologic features and a trend to higher transaminases at diagnosis...
  5. Dipstick Assay for Detection of Acetaminophen Protein Adducts
    Laura P James; Fiscal Year: 2010
    ..of the APAP level in serum within 24 hours of receiving the toxic dose, or by elevation of hepatic transaminases in serum, accompanied by a history of toxic dosing...
  6. MECHANISMS & INHIBITORS OF PYRIDOXAL-DEPENDENT ENZYMES
    DAVID METZLER; Fiscal Year: 1993
    ..The resulting knowledge will be important for rational design of drugs for inhibition of other transaminases. We also propose to do modeling of inhibitor action using an Evans & Sutherland graphics terminal...
  7. Longitudinal Studies of Primary Biliary Cirrhosis
    MARLYN MAYO; Fiscal Year: 2006
    ..Serum markers of fibrosis, age, bilirubin, albumin, prothrombin time, alkaline phosphatase, transaminases, and platelet count will be entered into a logistic model to derive a PBC-specific algorithm...
  8. VALPROATE RESPONSE IN AGGRESSIVE AUTISTIC ADOLESCENTS
    Jessica Hellings; Fiscal Year: 2002
    ..or previous liver disease, a history of ovarian cysts in girls, a low platelet count or raised liver transaminases. No other psychoactive maintenance medication will be allowed. Any level of MR (IQ<70) will be permissible...
  9. ALCOHOL-INDUCED HEPATIC P450s IN HCV/HIV OXIDATE INJURY
    STEVEN SHEDLOFSKY; Fiscal Year: 2003
    ..Daily blood ethanol, oxidative stress, transaminases, and fibrinogenesis will be assessed and changes correlated with changes in P450s...
  10. Role of SLc11a1 Gen in Warm Ischemia-Reperfusion Injury
    Samuel Wyllie; Fiscal Year: 2006
    ..Standard biochemical and histological techniques will be employed to evaluate blood transaminases and H&E liver sections, in conjunction with molecular techniques to monitor the protein and mRNA levels of ..
  11. TRYPTOPHAN METABOLISM IN MOSQUITOES
    Jianyong Li; Fiscal Year: 2007
    ..the roles both KAT and HKT play in mosquitoes, (2) to understand the functional evolution of these mosquito transaminases in comparison with counterpart enzymes from other species, and (3) to elucidate the structural basis of ..
  12. MPT INHIBITOR IND 50,820 IN FAMILIAL HYPERCHOLESTEROLEM*
    Marina Cuchel; Fiscal Year: 2009
    ..Three of the six subjects had substantial increases in liver transaminases, but levels rapidly decreased without a reduction in dosage in 2 of 3 patients...
  13. ALCOHOL, ACETAMINOPHEN, AND LIVER SINUSOID DYSFUNCTION
    ROBERT MC CUSKEY; Fiscal Year: 2004
    ..The toxic response to APAP is hallmarked by hemorrhagic centrilobular necrosis and towering levels of serum transaminases which are preceded by centrilobular microvascular injury and congestion...
  14. ANTIVIRAL & ANTIFIBROTIC LIVER THERAPY OF HCV+ DRINKERS
    Charles Lieber; Fiscal Year: 2005
    ..and which was found recently to be beneficial in HCV+ patients in terms of their circulating levels of transaminases. Fibrosis was not assessed, but documentation of the effects of PPC on fibrosis in HCV+ patients is being ..
  15. Ethanol effects on liver in self-administering primates
    Carol Cunningham; Fiscal Year: 2004
    ..by analyses of blood samples, which will include measurements of y-glutamyltransferase, aspartate and alanine transaminases, bilirubin, albumin, globulin and other blood components...
  16. Cloning, overexpression and characterization of novel alkene reductases
    Spiros Kambourakis; Fiscal Year: 2007
    ..have brought to the market more than 150 different enzymes formulated as active lyophilized powders, including transaminases, ketoreductases, nitrilases, amino acid dehydrogenases, aryl alcohol oxidases, human P450 oxidases, and many ..
  17. SURVEILLANCE METHODS FOR SOLVENT RELATED HEPATOTOXICITY
    CARL BRODKIN; Fiscal Year: 1999
    ..by parenchyma changes of steatosis and fibrosis, without associated necrotic changes detectable by transaminases, and will address a priority research area posed by the National Occupational Research Agenda for development ..
  18. PRODUCTION OF NON-NATURALLY OCCURRING AMINO ACIDS
    JAMES ROZZELL; Fiscal Year: 2001
    ..enzyme for preparative-scale reactions and establish the means for commercial production, genes encoding key transaminases from different classes and including D-transaminases will be cloned and expressed...
  19. EARLY NATURAL HISTORY OF HCV INFECTION AMONG IDUS
    Ronald Hershow; Fiscal Year: 2006
    ..Tests include: serum HCV RNA quantification, viral genotyping, and serum transaminases (ALT, AST)...
  20. ORGAN PRESERVATION WITH A RED BLOOD CELL SUBSTITUTE
    MARIA GAWRYL; Fiscal Year: 1992
    ..of glucose, lactate, amino acid N, urea N, release of K+ and tissue injury enzymes (lactodehydrogenase, transaminases), residual hepatic glycogen, ATP content and histological changes to delineate the extent of hepatocellular ..
  21. GARLIC THERAPY IN HYPERLIPIDEMIA CAUSED BY HAART
    Leanna Standish; Fiscal Year: 2000
    ..events associated with three escalating doses of Garlicin/TM and gathering preliminary data on the effects on fasting serum triglycerides, basal glucose and insulin levels as well as serum transaminases (ALT and AST).
  22. GARLIC THERAPY IN HYPERLIPIDEMIA CAUSED BY HAART
    Leanna Standish; Fiscal Year: 2001
    ..events associated with three escalating doses of Garlicin/TM and gathering preliminary data on the effects on fasting serum triglycerides, basal glucose and insulin levels as well as serum transaminases (ALT and AST).
  23. ON-LINE MONITOR OF HEPATIC PERFUSION USING FLUORESCENCE
    RAMEZ SHEHADA; Fiscal Year: 2000
    ..Currently, the measurement of liver enzymes (transaminases) and clotting factors (prothrombin) in the blood is the only reliable means for detecting perfusion-related ..
  24. Clostridial genome-scale metabolic and regulatory model of differentiation
    RYAN SENGER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  25. Influence of genotype on urinary oxalate excretion
    CARLA MONICO; Fiscal Year: 2007
    ..The applicant's long-term objective is to establish an investigative state-of the- art laboratory devoted to the study of the molecular genetics of hyperoxaluria. ..
  26. BRANCHED CHAIN AMINO ACID METABOLISM
    SUSAN HUTSON; Fiscal Year: 2008
    ..Results from this study will provide new insights into the role(s) played by BCAAs and high protein diets in weight loss and energy expenditure. ..
  27. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  28. MECHANISM OF GLUTATHIONE CONJUGATE DEPENDENT TOXICITY
    Arthur Cooper; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
  30. Nonalcoholic steatohepatitis and Cytochrome P450 Enzymes
    Naga P Chalasani; Fiscal Year: 2010
    ..Additionally, we examine the relationship between this enzyme activity and the degree of insulin resistance and lipid peroxidation. ..
  31. Macromolecular X-Ray Crystallography Instrument
    Neela Yennawar; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  32. Myocardial Protein Synthesis After Thermal Injury
    Charles Lang; Fiscal Year: 2005
    ..Overall, the research will elucidate the mechanisms by which myocardial protein synthesis is reduced after thermal injury, leading to the better understanding and treatment of the resulting cardiomyopathy. - ..
  33. Effect of hyperoxaluria on gene expression in the kidney
    Gill Rumsby; Fiscal Year: 2005
    ..By understanding why the disease progresses at different rates in patients with the same genetic abnormality, it may then be possible to devise strategies to slow down or prevent the clinical consequences of the disease. ..
  34. POST-PRANDIAL NUTRIENT PARTITIONING IN HUMANS
    Marc Andre Cornier; Fiscal Year: 2005
    ..These studies will provide insight into the biology that predisposes to weight gain and regain and will lay the groundwork for future studies of the abnormalities in nutrient metabolism that exist in obese and preobese individuals. ..
  35. Astatine-211 Conditioning for Nonmyeloablative Hematopoietic Stem Cell Allografts
    BRENDA MARIE SANDMAIER; Fiscal Year: 2010
    ..The final, and most important, studies will involve determining the quantity of 211At-labeled MAb required to obtain stable engraftment in HCT involvingDLA- haploidentical littermates. ..
  36. Assay kit for microsomal triglyceride transfer protein
    M Hussain; Fiscal Year: 2005
    ..In Phase II, we will simplify MTP purification, increase its stability, and develop a commercialization plan for the MTP assay kit. ..
  37. Phthalate Exposure and Pregnancy Outcome
    T Peter Stein; Fiscal Year: 2010
    ..The findings will have ramifications for public health, for the packaging trade and for the chemical industry. ..
  38. Occult Hepatitis B
    Michael Torbenson; Fiscal Year: 2007
    ..abstract_text> ..
  39. Role of Leucine Metabolism in Leucine Signaling
    CHRISTOPHER JOHN LYNCH; Fiscal Year: 2010
    ..The implication of our ongoing research is that blocking the first step in BCAA metabolism with a drug, or to a less extent increasing dietary BCAAs, will promote weight loss. ..
  40. Oxalate Handling in PAT1 and DRA Knockout Mice
    Marguerite Hatch; Fiscal Year: 2010
    ..Understanding oxalate handling in health and disease is essential for the development of potential pharmacological therapies. ..
  41. Degradation of Human CYP2E1 by the Proteasome
    Arthur Cederbaum; Fiscal Year: 2004
    ..Modulators of any of these newly identified steps may have therapeutic implications for individuals with high levels of CYP2E1. ..
  42. MAC 1/LFA 1/P150 95 AND PMN LEUKOCYTE FUNCTION
    Christie Ballantyne; Fiscal Year: 2002
    ..3. Characterize the contribution of each CD11 integrin on the host response to common bacterial and fungal pathogens in vitro and in vivo. ..
  43. Cell type gene delivery and alcoholic liver disease
    Michael Wheeler; Fiscal Year: 2006
    ..In addition, through didactic training, and interactions with his mentor and key faculty, the applicant will acquire new skills that will allow him to become a successful member of the alcohol research community. ..
  44. HIV Disease, Drug Abuse, & Nutrient Therapy in Botswana
    Marianna Baum; Fiscal Year: 2007
    ..Since antiretroviral therapies are not readily available to HIV infected adults in most countries in Africa, this study may provide an alternative approach to retard HIV progression. ..
  45. Mechanisms of experimental drug-induced hepatitis
    Dolores Njoku; Fiscal Year: 2007
    ..3) To confirm antigenic spread in TFA-CYP2E1-immunized mice using spleenocyte proliferation assays stimulated with ERp58 and ASGPR and analyzed as in Specific Aim 2. [unreadable] [unreadable] [unreadable] [unreadable]..
  46. Proteome of Glomerular Cells in Diabetic Nephropathy
    Paul Voziyan; Fiscal Year: 2007
    ..abstract_text> ..
  47. Adjuvant Therapy for Vascular Inflammation in Diabetes
    Sushil Jain; Fiscal Year: 2006
    ..abstract_text> ..
  48. Biomarker Genes in Mood Disorder: Lymphocyte and Brain
    MARQUIS VAWTER; Fiscal Year: 2006
    ..The potential use of biomarkers in lymphocytes will provide clinical researchers a tool to better address questions concerning diagnostic subgroups and treatment responders and non-responders. ..
  49. Counseling for Primary Care Office-Based Buprenorphine
    David Fiellin; Fiscal Year: 2009
    ..The results of this study will help define the role of professional evidence-based drug counseling in expanding access to treatment with Bup. ..
  50. GENERAL CLINICAL RESEARCH CENTERS ANNUAL MEETING
    Paul Watkins; Fiscal Year: 2006
    ..abstract_text> ..
  51. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2008
    ..Results from these experiments will support future microbiological studies of the role of this system in chlamydial infection and survival in the host cell. [unreadable] [unreadable] [unreadable]..
  52. REGULATION OF HEPATIC GLUTATHIONE SYNTHESIS
    Shelly Lu; Fiscal Year: 2008
    ..Our ultimate goal is to utilize this information to improve the treatment and prevent complications that may result from altered hepatic GSH synthesis. [unreadable] [unreadable]..
  53. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  54. Zinc Therapy in Zinc Deficient HIV+ Drug Users
    Marianna Baum; Fiscal Year: 2005
    ....
  55. EFFECTS OF ALCOHOL ON MOLECULAR AND CELLULAR PATHOLOGY
    Esteban Mezey; Fiscal Year: 2008
    ..The final objective is to instill in the trainee the ability to approach original questions of research relating to the pathogenesis of damaging effects of alcoholism on the body. ..
  56. Naltrexone Treatment in Pathologic Gambling Disorder
    SUCK KIM; Fiscal Year: 2004
    ..S. The implications of our study expand from PGD to other impulse control disorders, including compulsive shopping, kleptomania and possible alcoholism. ..
  57. CALCIUM OXALATE IN BIOLOGICAL SYSTEMS
    Marguerite Hatch; Fiscal Year: 2002
    ..abstract_text> ..
  58. IGF-I/ALS Trial
    Eric Sorenson; Fiscal Year: 2007
    ..The preliminary data from our prior trial allows design of a phase III treatment trial that will yield a definitive result about the treatment effect of IGF-I. ..
  59. Metabolic Consequences of Thymidine Sparing Regimens
    Grace A McComsey; Fiscal Year: 2010
    ..at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  60. Acupuncture to aid post-colectomy recovery in colorectal cancer patients
    Gary Deng; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..