nitrogenous group transferases

Summary

Summary: Enzymes that catalyze the transfer of nitrogenous groups, primarily amino groups, from a donor, generally an amino acid, to an acceptor, usually a 2-oxoacid. EC 2.6.

Top Publications

  1. ncbi Defining the importance of phosphatidylserine synthase 2 in mice
    Martin O Bergo
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 9100, USA
    J Biol Chem 277:47701-8. 2002
  2. ncbi Regulation of the Arabidopsis thaliana vitamin B6 biosynthesis genes by abiotic stress
    Sheri A Denslow
    Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695 7616, USA
    Plant Physiol Biochem 45:152-61. 2007
  3. pmc Vitamin B6 biosynthesis in higher plants
    Marina Tambasco-Studart
    Institute of Plant Sciences, Swiss Federal Institute of Technology ETH, Universitatstrasse 2, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 102:13687-92. 2005
  4. ncbi Translating organellar glutamine codons: a case by case scenario?
    Mathieu Frechin
    UPR Architecture et Réactivité de l ARN, Universite Louis Pasteur de Strasbourg, Strasbourg, France
    RNA Biol 6:31-4. 2009
  5. pmc Biogenesis of glutaminyl-mt tRNAGln in human mitochondria
    Asuteka Nagao
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 106:16209-14. 2009
  6. pmc Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis
    A W Curnow
    Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 95:12838-43. 1998
  7. pmc Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme
    Jing Wu
    Life Sciences Institute, Department of Biological Chemistry, University of Michigan, Ann Arbor, 48109, USA
    J Mol Biol 391:703-16. 2009
  8. pmc Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition
    Akiyoshi Nakamura
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    Nucleic Acids Res 38:672-82. 2010
  9. ncbi Her2p molecular modeling, mutant analysis and intramitochondrial localization
    Jose Ribamar Ferreira-Junior
    Escola de Artes, Ciências e Humanidades USP, Brazil
    Fungal Genet Biol 60:133-9. 2013
  10. ncbi Mechanistic studies of reaction coupling in Glu-tRNAGln amidotransferase
    K Y Horiuchi
    Chemical Enzymology and Antimicrobials Departments, DuPont Pharmaceuticals Company, Experimental Station, Wilmington, Delaware 19880, USA
    Biochemistry 40:6450-7. 2001

Research Grants

Scientific Experts

Detail Information

Publications135 found, 100 shown here

  1. ncbi Defining the importance of phosphatidylserine synthase 2 in mice
    Martin O Bergo
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141 9100, USA
    J Biol Chem 277:47701-8. 2002
    ..We conclude that Pss2 is responsible for the majority of serine exchange activity in in vitro assays, but a deficiency in this enzyme does not cause perturbations in phospholipid content or severe developmental abnormalities...
  2. ncbi Regulation of the Arabidopsis thaliana vitamin B6 biosynthesis genes by abiotic stress
    Sheri A Denslow
    Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695 7616, USA
    Plant Physiol Biochem 45:152-61. 2007
    ..Insight into regulation of the biosynthetic genes during abiotic stress could have important applications in the development of stress-tolerant crops...
  3. pmc Vitamin B6 biosynthesis in higher plants
    Marina Tambasco-Studart
    Institute of Plant Sciences, Swiss Federal Institute of Technology ETH, Universitatstrasse 2, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 102:13687-92. 2005
    ..Furthermore, and contrary to what was inferred previously, we show that the pathway appears to be cytosolic and is not localized to the plastid. Last, we report that the single PDX2 homolog is essential for plant viability...
  4. ncbi Translating organellar glutamine codons: a case by case scenario?
    Mathieu Frechin
    UPR Architecture et Réactivité de l ARN, Universite Louis Pasteur de Strasbourg, Strasbourg, France
    RNA Biol 6:31-4. 2009
    ..Indeed, QRS activity was found in protozoan mitochondria while AdT activity was characterized in plant organelles. The pathway for Q-tRNA(Q) synthesis in yeast and mammals mitochondria is still questionable...
  5. pmc Biogenesis of glutaminyl-mt tRNAGln in human mitochondria
    Asuteka Nagao
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 106:16209-14. 2009
    ....
  6. pmc Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis
    A W Curnow
    Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 95:12838-43. 1998
    ..radiodurans extracts, suggests that the gatCAB genes may be responsible for biosynthesis of asparagine in this asparagine prototroph...
  7. pmc Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme
    Jing Wu
    Life Sciences Institute, Department of Biological Chemistry, University of Michigan, Ann Arbor, 48109, USA
    J Mol Biol 391:703-16. 2009
    ..aeolicus GatB. The two sites enable GatCAB to first phosphorylate the misacylated tRNA substrate and then amidate the activated intermediate to form the cognate products, Gln-tRNA(Gln) or Asn-tRNA(Asn)...
  8. pmc Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition
    Akiyoshi Nakamura
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    Nucleic Acids Res 38:672-82. 2010
    ....
  9. ncbi Her2p molecular modeling, mutant analysis and intramitochondrial localization
    Jose Ribamar Ferreira-Junior
    Escola de Artes, Ciências e Humanidades USP, Brazil
    Fungal Genet Biol 60:133-9. 2013
    ..For instance, her2/ts11 mutant showed its fermentative growth impaired, and poorly rescued by GTF1 indicating that Her2p unknown function in the mitochondria outer membrane affects cell viability. ..
  10. ncbi Mechanistic studies of reaction coupling in Glu-tRNAGln amidotransferase
    K Y Horiuchi
    Chemical Enzymology and Antimicrobials Departments, DuPont Pharmaceuticals Company, Experimental Station, Wilmington, Delaware 19880, USA
    Biochemistry 40:6450-7. 2001
    ....
  11. pmc The kinase activity of the Helicobacter pylori Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase is sensitive to distal mutations in its putative ammonia tunnel
    Liangjun Zhao
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, United States
    Biochemistry 51:273-85. 2012
    ..A comparison of wild-type, T149V, and K89R AdT simulation results unmasks 59 common residues that are likely involved in connecting the two active sites...
  12. ncbi Complex assembly and metabolic profiling of Arabidopsis thaliana plants overexpressing vitamin B₆ biosynthesis proteins
    Jan Erik Leuendorf
    Angewandte Genetik, Freie Universitat Berlin, 14195 Berlin, Germany
    Mol Plant 3:890-903. 2010
    ..3. These findings provide first insights into in planta vitamin B₆ synthase complex assembly and new information on how the different PDX proteins affect plant metabolism...
  13. pmc From one amino acid to another: tRNA-dependent amino acid biosynthesis
    Kelly Sheppard
    Department of Molecular Biophysics, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 36:1813-25. 2008
    ..How these tRNA-dependent amino acid biosynthetic routes are integrated into overall metabolism may explain why they are still retained in so many organisms...
  14. ncbi Pyridoxine is required for post-embryonic root development and tolerance to osmotic and oxidative stresses
    Hao Chen
    Donald Danforth Plant Science Center, St Louis, MO 63132, USA
    Plant J 44:396-408. 2005
    ..Our study establishes a critical role of vitamin B6 in plant development and stress tolerance and suggests that vitamin B6 may represent a new class of antioxidant in plants...
  15. pmc Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation
    A W Curnow
    Department of Molecular Biophysics, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 94:11819-26. 1997
    ..Disruption of this operon is lethal. This demonstrates that transamidation is the only pathway to Gln-tRNAGln in B. subtilis and that glutamyl-tRNAGln amidotransferase is a novel and essential component of the translational apparatus...
  16. pmc Crystal structure of a transfer-ribonucleoprotein particle that promotes asparagine formation
    Mickael Blaise
    Department of Molecular Biology, CARB Centre, University of Aarhus, Arhus C, Denmark
    EMBO J 29:3118-29. 2010
    ..The transamidosome constitutes a transfer-ribonucleoprotein particle in which tRNAs serve the function of both substrate and structural foundation for a large molecular machine...
  17. ncbi Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions
    Takuhiro Ito
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 467:612-6. 2010
    ..The proposed mechanism explains how the transamidosome efficiently performs the two consecutive steps of Gln-tRNA(Gln) formation, with a low risk of releasing the unstable intermediate Glu-tRNA(Gln)...
  18. pmc Gln-tRNAGln synthesis in a dynamic transamidosome from Helicobacter pylori, where GluRS2 hydrolyzes excess Glu-tRNAGln
    Jonathan L Huot
    Departement de Biochimie, de microbiologie et de bio informatique, PROTEO et IBIS, Universite Laval, 1045 Av de la Médecine, Quebec, Quebec, Canada
    Nucleic Acids Res 39:9306-15. 2011
    ....
  19. ncbi tRNA-dependent asparagine formation in prokaryotes: characterization, isolation and structural and functional analysis of a ribonucleoprotein particle generating Asn-tRNA(Asn)
    Marc Bailly
    UPR 9002 Architecture et Réactivité de l ARN, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 Rue René Descartes and Université Louis Pasteur, F 67084 Strasbourg CEDEX, France
    Methods 44:146-63. 2008
    ....
  20. pmc Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants
    Claire Pujol
    Institut de Biologie Moléculaire des Plantes Unité Propre de Recherche 2357 du Centre National de la Recherche Scientifique, Université Louis Pasteur Strasbourg 1, Centre National de la Recherche Scientifique, Strasbourg, France
    Proc Natl Acad Sci U S A 105:6481-5. 2008
    ..Moreover, the mitochondrial pathway of Gln-tRNA(Gln) formation is shared with chloroplasts as both the GluRS, and the three AdT subunits are dual-imported into mitochondria and chloroplasts...
  21. pmc The archaeal transamidosome for RNA-dependent glutamine biosynthesis
    Theodoros Rampias
    Department of Molecular Biophysics and Biochemistry and Department of Chemistry, Yale University, New Haven, CT 06511, USA
    Nucleic Acids Res 38:5774-83. 2010
    ..The differences between the two transamidosomes may be a consequence of the fact that ND-GluRS is a class I aminoacyl-tRNA synthetase, while ND-AspRS belongs to the class II family...
  22. ncbi PDX1 is essential for vitamin B6 biosynthesis, development and stress tolerance in Arabidopsis
    Olca Titiz
    ETH Zurich, Institute of Plant Science, 8092 Zurich, Switzerland
    Plant J 48:933-46. 2006
    ..Furthermore, we provide evidence that in addition to being essential for plant growth and development, vitamin B6 also plays a role in stress tolerance and photoprotection of plants...
  23. ncbi Mechanism of a GatCAB amidotransferase: aspartyl-tRNA synthetase increases its affinity for Asp-tRNA(Asn) and novel aminoacyl-tRNA analogues are competitive inhibitors
    Jonathan L Huot
    Centre de Recherche sur la Fonction, la Structure et l Ingénierie des Protéines, Departement de Biochimie et de Microbiologie, et Département de Chimie, Universite Laval, Quebec, Quebec, Canada G1K 7P4
    Biochemistry 46:13190-8. 2007
    ....
  24. ncbi The transamidosome: a dynamic ribonucleoprotein particle dedicated to prokaryotic tRNA-dependent asparagine biosynthesis
    Marc Bailly
    UPR Architecture et Réactivité de l ARN, Universite Louis Pasteur de Strasbourg, CNRS, Institut de Biologie Moleculaire et Cellulaire, 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    Mol Cell 28:228-39. 2007
    ..We propose that formation of a ribonucleoprotein is a general feature for tRNA-dependent amino acid biosynthetic pathways that are remnants of earlier stages when amino acid synthesis and tRNA aminoacylation were coupled...
  25. pmc On the evolution of the tRNA-dependent amidotransferases, GatCAB and GatDE
    Kelly Sheppard
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Mol Biol 377:831-44. 2008
    ..Archaeal GatCAB, on the other hand, has not favored a distinct tRNA(Asn), suggesting that tRNA(Asn) recognition is not a major barrier to the retention of asparaginyl-tRNA synthetase in many Archaea...
  26. pmc Methanothermobacter thermautotrophicus tRNA Gln confines the amidotransferase GatCAB to asparaginyl-tRNA Asn formation
    Kelly Sheppard
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Mol Biol 377:845-53. 2008
    ..pylori or B. subtilis, and M. thermautotrophicus Glu-tRNA(Asn). Thus, M. thermautotrophicus encodes two amidotransferases, each with its own activity, GatDE for Gln-tRNA and GatCAB for Asn-tRNA synthesis...
  27. ncbi The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln
    Kelly Sheppard
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 282:11866-73. 2007
    ..Therefore, bacterial amide aminoacyl-tRNA formation is intimately tied to amide amino acid metabolism...
  28. ncbi Growth inhibition of Escherichia coli during heterologous expression of Bacillus subtilis glutamyl-tRNA synthetase that catalyzes the formation of mischarged glutamyl-tRNA1 Gln
    Ji Won Baick
    Department of Food Science and Technology, Dongguk University, Seoul 100 715, Korea
    J Microbiol 42:111-6. 2004
    ..coli GlutRNA1 Gln, and converts it to the cognate Gln-tRNA1 Gln species. B. subtilis GluRS-dependent Glu-tRNA1 Gln formation may cause growth inhibition in the transformed E. coli strain, possibly due to abnormal protein synthesis...
  29. pmc Deinococcus glutaminyl-tRNA synthetase is a chimer between proteins from an ancient and the modern pathways of aminoacyl-tRNA formation
    Marzanna Deniziak
    UPR 9002 Architecture et Réactivité de l ARN, Universite Louis Pasteur, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    Nucleic Acids Res 35:1421-31. 2007
    ..However, the presence of Yqey in free state in organisms lacking GlnRS, suggests that this domain may exert additional cellular functions...
  30. ncbi Structural basis for tRNA-dependent amidotransferase function
    Emmanuelle Schmitt
    Laboratoire de Biochimie, Unité Mixte de Recherche 7654, CNRS Ecole Polytechnique, F 91128 Palaiseau cedex, France
    Structure 13:1421-33. 2005
    ..Comparison of GatD and L-asparaginase structures shows how the motion of a beta hairpin region containing a crucial catalytic threonine may control the overall reaction cycle of GatDE...
  31. ncbi A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans
    J C Salazar
    , , Facultad de Medicina, Universidad de Chile, Santiago, Chile
    FEBS Lett 500:129-31. 2001
    ..These data suggest that the transamidation pathway is responsible for the formation of Gln-tRNA and Asn-tRNA in A. ferrooxidans...
  32. pmc Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens
    Antoine P Maillard
    Laboratoire de Recherche Moleculaire sur les Antibiotiques, INSERM U655, Universite Paris 6, Paris, France
    J Bacteriol 187:3833-8. 2005
    ....
  33. ncbi Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)
    Subray S Hegde
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:22861-7. 2003
    ..Site-directed mutagenesis identified Asp109 as a candidate for the catalytic base and Glu320 plays an additional important role in the catalytic function of the enzyme...
  34. pmc A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine
    Marc Bailly
    Département Machineries Traductionnelles, UPR 9002 Architecture et Réactivité de l ARN, Institut de Biologie Moléculaire et Cellulaire du CNRS 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    Nucleic Acids Res 34:6083-94. 2006
    ..In the light of these results we propose that bacterial and archaeal AdTs use kingdom-specific signals to catalyze the tRNA-dependent formations of Asn and Gln...
  35. ncbi Domain-specific recruitment of amide amino acids for protein synthesis
    D L Tumbula
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Nature 407:106-10. 2000
    ..Closer inspection of the two amidotransferases reveals that each of them recruited a metabolic enzyme to aid its function; this provides direct evidence for a relationship between amino-acid metabolism and protein biosynthesis...
  36. pmc Analysis of the Arabidopsis rsr4-1/pdx1-3 mutant reveals the critical function of the PDX1 protein family in metabolism, development, and vitamin B6 biosynthesis
    Susan Wagner
    Angewandte Genetik, Freie Universitat Berlin, 14195 Berlin, Germany
    Plant Cell 18:1722-35. 2006
    ..In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants...
  37. ncbi Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition
    Sabrina Biarrotte-Sorin
    Laboratoire de Minéralogie Cristallographie de Paris, Universite Paris 6, 4 place Jussieu, Paris Cedex 05, 75252, France
    Structure 12:257-67. 2004
    ....
  38. pmc A thin-layer electrophoretic assay for Asp-tRNAAsn/Glu-tRNAGln amidotransferase
    Terry J T Cathopoulis
    Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA
    Anal Biochem 360:151-3. 2007
  39. pmc Characterization of the glutamyl-tRNA(Gln)-to-glutaminyl-tRNA(Gln) amidotransferase reaction of Bacillus subtilis
    M A Strauch
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    J Bacteriol 170:916-20. 1988
    ..In addition, there was no inhibition by inhibitors of either glutamine synthetase or other amidotransferases. A unique, rather labile activity seems to be required for this reaction...
  40. ncbi Mutagenesis and mechanism-based inhibition of Streptococcus pyogenes Glu-tRNAGln amidotransferase implicate a serine-based glutaminase site
    Mark R Harpel
    Chemical Enzymology Department, Bristol Myers Squibb Company, Experimental Station, Route 141 and Henry Clay Road, Wilmington, Delaware 19880, USA
    Biochemistry 41:6398-407. 2002
    ....
  41. ncbi The Pdx1 family is structurally and functionally conserved between Arabidopsis thaliana and Ginkgo biloba
    Jan E Leuendorf
    Angewandte Genetik, Freie Universitat, Berlin, Germany
    FEBS J 275:960-9. 2008
    ..Overall, this study provides a first description of Ginkgo vitamin B6 metabolism, and demonstrates a high degree of conservation between Ginkgo and Arabidopsis...
  42. pmc Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase
    Mario H Barros
    Department of Microbiology, University of Sao Paulo, 05508 900 Sao Paulo, Brazil
    J Biol Chem 286:32937-47. 2011
    ..Senger, B., Brayé, M., Kern, D., Martin, R. P., and Becker, H. D. (2009) Genes Dev. 23, 1119-1130)...
  43. ncbi Structural basis of RNA-dependent recruitment of glutamine to the genetic code
    Hiroyuki Oshikane
    Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama shi, Kanagawa 226 8501, Japan
    Science 312:1950-4. 2006
    ..tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code...
  44. ncbi The heterotrimeric Thermus thermophilus Asp-tRNA(Asn) amidotransferase can also generate Gln-tRNA(Gln)
    H D Becker
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    FEBS Lett 476:140-4. 2000
    ....
  45. pmc Vitamer levels, stress response, enzyme activity, and gene regulation of Arabidopsis lines mutant in the pyridoxine/pyridoxamine 5'-phosphate oxidase (PDX3) and the pyridoxal kinase (SOS4) genes involved in the vitamin B6 salvage pathway
    Eugenia González
    Department of Plant Biology, North Carolina State University, Raleigh, North Carolina 27695 7612, USA
    Plant Physiol 145:985-96. 2007
    ..Increased expression of salvage and some of the de novo pathway genes was observed in both the pdx3 and sos4 mutants. In all mutants, increased expression was more dramatic for the salvage pathway genes...
  46. ncbi Crystallization and preliminary X-ray characterization of the atypical glutaminyl-tRNA synthetase from Deinococcus radiodurans
    Marzanna Agnieszka Deniziak
    Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    Acta Crystallogr D Biol Crystallogr 60:2361-3. 2004
    ..Orthorhombic crystals were obtained that belong to space group P2(1)2(1)2(1) and diffract to 2.3 A resolution. The crystal structure was solved by molecular replacement using the structure of E. coli GlnRS as a search model...
  47. pmc Functional analysis of PDX2 from Arabidopsis, a glutaminase involved in vitamin B6 biosynthesis
    Marina Tambasco-Studart
    Institute of Plant Sciences, Eidgenossische Technische Hochschule Zurich, 8092 Zurich, Switzerland
    Plant Physiol 144:915-25. 2007
    ..Based on a model of the PDX1/PDX2 complex, mutation of a single residue uncouples enzyme coordination and in turn provides tangible evidence for the existence of the recently proposed ammonia tunnel through the core of PDX1...
  48. pmc Characterization of LtsA from Rhodococcus erythropolis, an enzyme with glutamine amidotransferase activity
    Yasuo Mitani
    Proteolysis and Protein Turnover Research Group, Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology AIST, Tsukisamu Higashi, Toyohira ku, Sapporo 062 8517, Japan
    J Bacteriol 187:2582-91. 2005
    ..Taken together, these results suggest that LtsA is critically involved in mediating lysozyme resistance in R. erythropolis cells...
  49. pmc Crystal structure of glutamine amidotransferase from Thermotoga maritima
    S Korolev
    Biosciences Division and Structural Biology Center, Argonne National Laboratory, Argonne, Illinois 60439, USA
    Proteins 49:420-2. 2002
  50. ncbi Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit
    I P Crawford
    Department of Microbiology, University of Iowa, Iowa City 52242
    Mol Biol Evol 3:436-48. 1986
    ..We have also determined 490 bp of DNA sequence distal to trpG but have not ascertained the function of this segment, though it is rich in dyad symmetries...
  51. ncbi Deconstruction of the catalytic array within the amidotransferase subunit of carbamoyl phosphate synthetase
    X Huang
    Department of Chemistry, Texas A and M University, College Station, Texas, 77843, USA
    Biochemistry 38:15909-14. 1999
    ..It is concluded that contrary to the original proposal for the catalytic triad, Glu-355 is not an essential residue for catalysis. The results are consistent with Ser-47 and Gln-273 playing significant roles in the binding of glutamine...
  52. ncbi Evolution of glutamine amidotransferase genes. Nucleotide sequences of the pabA genes from Salmonella typhimurium, Klebsiella aerogenes and Serratia marcescens
    J B Kaplan
    J Mol Biol 183:327-40. 1985
    ....
  53. ncbi Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme
    C Avila
    Biochemistry and Physiology Department, Rothamsted Experimental Station, AFRC Institute of Arable Crops Research, Harpenden, Herts, UK
    Planta 189:475-83. 1993
    ..7 kb, the other smaller. Using a set of wheat-barley telosomic addition lines we have assigned the Fd-GOGAT structural locus to the short arm of chromosome 2...
  54. ncbi Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis
    T J Klem
    Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, Indiana 47907 1333
    Biochemistry 32:5177-86. 1993
    ..The mechanistic significance of this glutaminase activity compared to other trpG type glutamine amidotransferases is discussed...
  55. ncbi Differential effects of GLUT1 or GLUT4 overexpression on hexosamine biosynthesis by muscles of transgenic mice
    M G Buse
    Department of Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 271:23197-202. 1996
    ....
  56. ncbi Identification of a trpG-related glutamine amide transfer domain in Escherichia coli GMP synthetase
    H Zalkin
    J Biol Chem 260:3350-4. 1985
    ..coli amidophosphoribosyltransferase and human hypoxanthine-guanine phosphoribosyltransferase, suggest a structurally homologous segment which may constitute a GMP binding site...
  57. pmc The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli
    J G Lawrence
    Department of Biology, University of Utah, Salt Lake City 84112, USA
    J Bacteriol 177:6371-80. 1995
    ..The cob operon of E. coli may be related to cobalamin synthetic genes now found among non-Salmonella enteric bacteria...
  58. ncbi Glucose induces increases in levels of the transcriptional repressor Id2 via the hexosamine pathway
    Line Mariann Grønning
    Department of Biochemistry and Molecular Biology, Guys Hospital, Kings College, London, United Kingdom
    Am J Physiol Endocrinol Metab 290:E599-606. 2006
    ..In summary, these studies define a new mechanism by which glucose uses the hexosamine pathway to regulate gene expression by increasing levels of a transcriptional repressor...
  59. ncbi Inhibition of Helicobacter pylori aminoacyl-tRNA amidotransferase by chloramphenicol analogs
    Christian Balg
    Departement de Chimie, PROTEO, Faculte des sciences et de genie, Universite Laval, Quebec, Canada G1V 0A6
    Bioorg Med Chem 18:7868-72. 2010
    ..Compound 7a was identified as the most active competitive inhibitor of the transamidase activity with respect to Asp-tRNA(Asn) (K(m)=2μM), with a K(i) value of 27μM...
  60. ncbi Phosphatidylserine synthase-1 and -2 are localized to mitochondria-associated membranes
    S J Stone
    Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 275:34534-40. 2000
    ..These data leave open the question of which enzyme imparts PSS activity to the ER and suggest that a third isoform of PSS might be located in the ER...
  61. pmc The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis
    Cyril Moccand
    From the Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland and
    J Biol Chem 289:8203-16. 2014
    ..Analysis of pdx1.2 knockdown lines demonstrates that boosting vitamin B6 content is dependent on PDX1.2, revealing that this pseudoenzyme acts as a positive regulator of vitamin B6 biosynthesis during such stress conditions in plants. ..
  62. ncbi Natural insertion of the bro-1 β-lactamase gene into the gatCAB operon affects Moraxella catarrhalis aspartyl-tRNA(Asn) amidotransferase activity
    Pierre Marie Akochy
    Departement de Biochimie, de microbiologie et de bio informatique, Universite Laval, QC G1V 0A6, Canada
    Microbiology 158:2363-71. 2012
    ..We conclude that the bro gene insertion has altered the kinetic parameters of Asp-tRNA(Asn) amidotransferase, and we propose a model for gatA evolution after the insertion of bro-1 at the carboxyl end of gatA...
  63. ncbi Two-step pathway to aminoacylated tRNA
    John J Perona
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, California 93106, USA
    Structure 13:1397-8. 2005
    ....
  64. ncbi A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase
    Jianghai Zhu
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 280:27914-23. 2005
    ..The distribution of conserved surfaces in the PdxS dodecamer was used to predict a docking site for the glutaminase partner, PdxT...
  65. pmc A Link between ethylene and auxin uncovered by the characterization of two root-specific ethylene-insensitive mutants in Arabidopsis
    Anna N Stepanova
    Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
    Plant Cell 17:2230-42. 2005
    ..This study provides a mechanistic explanation for the root-specific ethylene insensitivity of wei2 and wei7, illustrating how interactions between hormones can be used to achieve response specificity...
  66. pmc Cellular content of UDP-N-acetylhexosamines controls hyaluronan synthase 2 expression and correlates with O-linked N-acetylglucosamine modification of transcription factors YY1 and SP1
    Tiina A Jokela
    Institute of Biomedicine, University of Eastern Finland, FIN 70211 Kuopio, Finland
    J Biol Chem 286:33632-40. 2011
    ....
  67. pmc Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance
    Padma Bhonagiri
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Endocrinology 152:3373-84. 2011
    ..Furthermore, these data are consistent with the PM cholesterol accrual/toxicity as a mechanistic basis of the HBP-induced defects in PIP(2)/F-actin structure and impaired glucose transporter-4 regulation...
  68. ncbi Suppression of Glutamine:fructose-6-phosphate amidotransferase-1 inhibits adipogenesis in 3T3-L1 adipocytes
    Tusty Jiuan Hsieh
    Department of Medical Genetics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC
    J Cell Physiol 227:108-15. 2012
    ..Our results suggest that GFAT-1 plays a critical role in modulating adipogenesis via the regulation of protein O-GlcNAcylation in adipocytes...
  69. ncbi Enhanced levels of vitamin B(6) increase aerial organ size and positively affect stress tolerance in Arabidopsis
    Maja Raschke
    ETH Zurich, Institute of Plant Sciences, 8092 Zurich, Switzerland
    Plant J 66:414-32. 2011
    ..In particular, the plants show an increased resistance to paraquat and photoinhibition, and they attenuate the cell death response observed in the conditional flu mutant...
  70. pmc The glycosylation pathway of Eimeria tenella is upregulated during gametocyte development and may play a role in oocyst wall formation
    Robert A Walker
    Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, New South Wales 2007, Australia
    Eukaryot Cell 9:127-35. 2010
    ..tenella sexual stages and may play a role in directing a number of macrogamete proteins to the developing oocyst wall...
  71. pmc Aminoacyl-tRNA recognition by the FemXWv transferase for bacterial cell wall synthesis
    Matthieu Fonvielle
    Centre de Recherche des Cordeliers, LRMA, Equipe 12, INSERM, Universite Pierre et Marie Curie Paris 6, France
    Nucleic Acids Res 37:1589-601. 2009
    ..These results indicate that aminoacyl-tRNA recognition by FemX(Wv) is distinct from other components of the translation machinery and relies on the exclusion of bulky amino acids and of the sequence of tRNA(Gly) from the active site...
  72. ncbi Conformational changes in ammonia-channeling glutamine amidotransferases
    Stephane Mouilleron
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS Bâtiment 34, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France
    Curr Opin Struct Biol 17:653-64. 2007
    ..Structural information shows how flexible loops of the synthase and glutaminase domains move to shield the two catalytic sites and anchor the substrates, and how the ammonia channel forms and opens or closes...
  73. ncbi Synthesis of phosphatidylserine by base exchange in Triton-insoluble floating fractions from rat cerebellum
    Sandra Buratta
    Department of Internal Medicine, Biochemistry Section, University of Perugia, Perugia, Italy
    J Neurochem 103:942-51. 2007
    ..The capability of SBEE to utilize not only serine but also ethanolamine, as free exchanging base, suggests a mechanism for modulating in loco PS concentration...
  74. ncbi Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway
    Kristin E Burns
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    J Am Chem Soc 127:3682-3. 2005
    ..The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified...
  75. ncbi Biosynthetic regulation and intracellular transport of phosphatidylserine in mammalian cells
    Osamu Kuge
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640
    J Biochem 133:397-403. 2003
    ..Here we describe the current understanding of the regulation of PtdSer biosynthesis and the transport of PtdSer from the ER to the mitochondria in mammalian cells...
  76. pmc Control of phosphatidylserine biosynthesis through phosphatidylserine-mediated inhibition of phosphatidylserine synthase I in Chinese hamster ovary cells
    O Kuge
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162, Japan
    Proc Natl Acad Sci U S A 95:4199-203. 1998
    ..These results indicated that the inhibition of PtdSer synthase I by PtdSer is essential for the maintenance of a normal PtdSer level in CHO-K1 cells and that Arg-95 of the synthase is a crucial residue for the inhibition...
  77. ncbi The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases
    M Janczarek
    Department of General Microbiology, M Curie Sklodowska University, Lublin, Poland
    FEMS Microbiol Lett 173:319-25. 1999
    ..A mutant strain was also defective in nitrogen fixation suggesting a regulatory role of pssB in symbiosis with clover...
  78. ncbi Thr-431 and Arg-433 are part of a conserved sequence motif of the glutamine amidotransferase domain of CTP synthases and are involved in GTP activation of the Lactococcus lactis enzyme
    Martin Willemoes
    Center for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen Ø, Denmark
    J Biol Chem 278:9407-11. 2003
    ..The mutant enzymes T431V and R433M apparently had not lost the ability to bind GTP, but the signal transmitted through the enzyme to the active sites upon binding of the allosteric effector was clearly disrupted in the mutant enzymes...
  79. pmc In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes
    L A Maggio-Hall
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706 1567, USA
    Proc Natl Acad Sci U S A 96:11798-803. 1999
    ....
  80. pmc A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be involved in tryptophan transport
    J P Sarsero
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    J Bacteriol 182:2329-31. 2000
    ..Production of the yhaG transcript in vivo was found to compete for the binding of TRAP to other known TRAP binding sites. YhaG is likely to be a transmembrane protein involved in tryptophan transport...
  81. ncbi Group B streptococcus (GBS) modifies macrophage phosphatidylserine metabolism during induction of apoptosis
    Sandra Buratta
    Department of Internal Medicine, Biochemistry Section, University of Perugia, 06122 Perugia, Italy
    FEBS Lett 520:68-72. 2002
    ..These results confirm that induction of apoptosis involves a modification of PtdSer metabolism and point out the typical features of the GBS-induced apoptosis with respect to other models of apoptosis...
  82. ncbi Apoptosis by phosphatidylserine in mammalian cells
    Kazuo Emoto
    Department of Molecular Biodynamics, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Subcell Biochem 36:61-77. 2002
  83. ncbi Glutamyl-gamma-boronate inhibitors of bacterial Glu-tRNA(Gln) amidotransferase
    C P Decicco
    Department of Medicinal Chemistry, DuPont Pharmaceuticals Company, Experimental Station, PO Box 80400, Wilmington, DE 19880, USA
    Bioorg Med Chem Lett 11:2561-4. 2001
    ..Nonetheless, growth inhibition of organisms that require Glu-AdT by the most potent enzyme inhibitors appears to validate mechanism-based inhibitor design of Glu-AdT as an approach to antimicrobial development...
  84. ncbi Cloning and expression of mouse liver phosphatidylserine synthase-1 cDNA. Overexpression in rat hepatoma cells inhibits the CDP-ethanolamine pathway for phosphatidylethanolamine biosynthesis
    S J Stone
    Lipid and Lipoprotein Research Group, University of Alberta, Edmonton, AB T6G 2S2, Canada
    J Biol Chem 273:7293-302. 1998
    ..These data suggest that (i) cellular levels of PtdSer and PtdEtn are tightly controlled, and (ii) the metabolism of PtdSer and PtdEtn is coordinately regulated to maintain phospholipid homeostasis...
  85. ncbi Topography of glycerolipid synthetic enzymes. Synthesis of phosphatidylserine, phosphatidylinositol and glycerolipid intermediates occurs on the cytoplasmic surface of rat liver microsomal vesicles
    L M Ballas
    Biochim Biophys Acta 665:586-95. 1981
    ..M. and Bell, R.M., Biochim. Biophys. Acta 602, (1980) 578-590) indicate that the synthesis of the major cellular glycerolipids occurs asymmetrically on the cytoplasmic surface of the endoplasmic reticulum...
  86. ncbi Glutamine amidotransferase function. Replacement of the active-site cysteine in glutamine phosphoribosylpyrophosphate amidotransferase by site-directed mutagenesis
    P Mantsala
    J Biol Chem 259:14230-6. 1984
    ..Alternative enzymatic and autocatalytic processing mechanisms were considered. The available evidence favors autocatalytic NH2-terminal undecapeptide processing...
  87. ncbi The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor
    Hee Jeon Hong
    Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom
    J Biol Chem 280:13055-61. 2005
    ..Because only VanK can recognize D-Lac-containing precursors, vancomycin-induced expression of VanHAX in a vanK mutant is lethal, and so vanK is required for vancomycin resistance...
  88. pmc Cloning and characterization of the trpC gene from an aflatoxigenic strain of Aspergillus parasiticus
    J S Horng
    Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824
    Appl Environ Microbiol 55:2561-8. 1989
    ..This gene should be useful as a selectable marker in developing a DNA-mediated transformation system to analyze the aflatoxin biosynthetic pathway of A. parasiticus...
  89. ncbi The rudimentary gene of Drosophila melanogaster encodes four enzymic functions
    J N Freund
    J Mol Biol 193:1-13. 1987
    ..We have found that the corresponding mammalian gene, the CAD gene, exhibits a similar functional organization, and we propose schemes for the evolution of the corresponding coding sequences...
  90. ncbi Structure and expression of the murine phosphatidylserine synthase-1 gene
    B Sturbois-Balcerzak
    Department of Medicine and the Canadian Institutes for Health Research Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 276:8205-12. 2001
    ..Isolation of the murine PSS1 gene is a step toward generation of genetically modified mouse models that will help to understand the functions of PSS1 and PSS2 in animal biology...
  91. ncbi Mechanism for acivicin inactivation of triad glutamine amidotransferases
    S V Chittur
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
    Biochemistry 40:876-87. 2001
    ..Stabilization of the imine-thioether intermediate by the oxyanion hole in triad glutamine amidotransferases appears to confer the high degree of specificity for acivicin inhibition and relates to a common mechanism for inactivation...
  92. pmc The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, L-tryptophan
    G Spraggon
    Genomics Institute of the Novartis Research Foundation, 3115 Merryfield Row, La Jolla, CA 92121 1115, USA
    Proc Natl Acad Sci U S A 98:6021-6. 2001
    ..Our findings are discussed in terms of the previously described AS structure of S. solfataricus, mutational data obtained from enteric bacteria, and the enzyme's mechanism of action...
  93. pmc An apparent Bacillus subtilis folic acid biosynthetic operon containing pab, an amphibolic trpG gene, a third gene required for synthesis of para-aminobenzoic acid, and the dihydropteroate synthase gene
    J Slock
    Department of Biology, King s College, Wilkes Barre, Pennsylvania 18711
    J Bacteriol 172:7211-26. 1990
    ..We propose that this regulation is at the level of translation. This hypothesis is supported by the finding of an apparent Mtr-binding site which overlaps with the trpG ribosome-binding site...
  94. ncbi Cloning of a Chinese hamster ovary (CHO) cDNA encoding phosphatidylserine synthase (PSS) II, overexpression of which suppresses the phosphatidylserine biosynthetic defect of a PSS I-lacking mutant of CHO-K1 cells
    O Kuge
    Department of Biochemistry and Cell Biology, National Institute of Health, Toyama 1 23 1, Shinjuku ku, Tokyo 162, Japan
    J Biol Chem 272:19133-9. 1997
    ..These results suggested that the pssB cDNA encoded the second PtdSer synthase PSS II, which catalyzed the serine and ethanolamine base exchange, but not the choline base exchange...
  95. ncbi Role of the hexosamine biosynthetic pathway in diabetic nephropathy
    E D Schleicher
    Department of Internal Medicine, University of Tubingen, Germany
    Kidney Int Suppl 77:S13-8. 2000
    ..Current data indicate that the flux through the hexosamine pathway, regulated by GFAT, may be causally involved in the development of diabetic vascular disease, particularly diabetic nephropathy...
  96. ncbi Enhancement of vitamin B(6) levels in seeds through metabolic engineering
    Hao Chen
    Donald Danforth Plant Science Center, St Louis, MO, USA
    Plant Biotechnol J 7:673-81. 2009
    ..Our work demonstrates that it is feasible to enhance vitamin B(6) content in seeds by metabolic engineering...
  97. ncbi Deletion analysis of domain independence in the TRP1 gene product of Neurospora crassa
    M S Walker
    Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225
    Mol Gen Genet 223:49-57. 1990
    ....
  98. ncbi As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe
    M Lollier
    Laboratoire de Microbiologie et de Genetique, URA n 1481 Université Louis Pasteur CNRS, Strasbourg, France
    Curr Genet 28:138-49. 1995
    ..They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product...
  99. pmc Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly
    Angelyn Larkin
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 49:7227-37. 2010
    ..aeruginosa infection...
  100. ncbi Isolation and characterization of the Aspergillus niger trpC gene
    A Kos
    Gene 39:231-8. 1985
    ..These experiments suggest that the organization of the A. niger trpC gene is identical to that of the analogous A. nidulans trpC and the Neurospora crassa trp-1 genes...
  101. pmc Three-dimensional profiles from residue-pair preferences: identification of sequences with beta/alpha-barrel fold
    M Wilmanns
    Molecular Biology Institute, University of California, Los Angeles 90024 1570
    Proc Natl Acad Sci U S A 90:1379-83. 1993
    ....

Research Grants2

  1. MECHANISMS AND CONSEQUENCES OF DELETERIOUS EVOLUTION IN BACTERIA
    Jennifer J Wernegreen; Fiscal Year: 2010
    ..By deciphering fundamental mechanisms that influence bacterial genome evolution, this study will contribute to a predictive framework for understanding how microbes evolve. ..
  2. PHOSPHOLIPID METABOLISM AND MEMBRANE FUNCTION
    GEORGE CARMAN; Fiscal Year: 2009
    ..Zinc transport function will be addressed with mutants defective in phospholipid synthesis, and with Zrtlp reconstituted into unilamellar vesicles with varying phospholipid composition. ..