pentosyltransferases

Summary

Summary: Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another. (Dorland, 28th ed) EC 2.4.2.

Top Publications

  1. pmc vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12
    J M Durand
    Department of Bacteriology, University of Tokyo, Japan
    J Bacteriol 176:4627-34. 1994
  2. ncbi Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 57:732-46. 2009
  3. ncbi The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol
    A Telenti
    Institute for Medical Microbiology, University of Bern, Switzerland
    Nat Med 3:567-70. 1997
  4. ncbi A new function for a common fold: the crystal structure of quinolinic acid phosphoribosyltransferase
    J C Eads
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Structure 5:47-58. 1997
  5. ncbi Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA
    S Noguchi
    J Biol Chem 257:6544-50. 1982
  6. pmc Transfer of embB codon 306 mutations into clinical Mycobacterium tuberculosis strains alters susceptibility to ethambutol, isoniazid, and rifampin
    Hassan Safi
    Division of Infectious Disease and Center for Emerging Pathogens, Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    Antimicrob Agents Chemother 52:2027-34. 2008
  7. pmc The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol
    A E Belanger
    Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Proc Natl Acad Sci U S A 93:11919-24. 1996
  8. ncbi Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination
    Alexander G Maier
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    Mol Biochem Parasitol 150:118-21. 2006
  9. ncbi In vivo cancer gene therapy by adenovirus-mediated transfer of a bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion gene
    P Erbs
    Transgene S A Strasbourg, France
    Cancer Res 60:3813-22. 2000
  10. pmc Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo
    Kirti Sharma
    Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India
    J Bacteriol 188:2936-44. 2006

Research Grants

  1. THE MUTAGENICITY OF MINERAL FIBERS
    TOM HEI; Fiscal Year: 2006
  2. MECHANISMS OF FIBER CARCINOGENESIS
    TOM HEI; Fiscal Year: 2001
  3. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
  4. GENE DISCOVERY IN TOXOPLASMA GONDII
    L Sibley; Fiscal Year: 2003
  5. Development of New GI-specific Histone Deacetylase Inhi*
    Scott Rajski; Fiscal Year: 2006
  6. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
  7. Mucosal Immunity to Toxoplasma gondii
    L Sibley; Fiscal Year: 2007
  8. MECHANISMS OF ARSENIC CARCINOGENESIS
    TOM HEI; Fiscal Year: 2005
  9. Structural Biology of RNA
    ADRIAN FERRE D AMARE; Fiscal Year: 2009
  10. Molecular dynamics studies of muscle proteins
    EDWARD F PATE; Fiscal Year: 2010

Detail Information

Publications222 found, 100 shown here

  1. pmc vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12
    J M Durand
    Department of Bacteriology, University of Tokyo, Japan
    J Bacteriol 176:4627-34. 1994
    ..coli strains, consistent with the hypothesis that the vacC (tgt) gene contributes to the pathogenicity of Shigella flexneri...
  2. ncbi Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 57:732-46. 2009
    ..These characteristics are similar to those of irx9 and irx14, mutants that are believed to be defective in xylan chain elongation, and suggests that IRX10 and IRX10-L also play a role in elongation of the xylan backbone...
  3. ncbi The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol
    A Telenti
    Institute for Medical Microbiology, University of Bern, Switzerland
    Nat Med 3:567-70. 1997
    ..Further characterization of these proteins might provide information on targets for new chemotherapeutic agents and might help development of diagnostic strategies for the detection of resistant M. tuberculosis...
  4. ncbi A new function for a common fold: the crystal structure of quinolinic acid phosphoribosyltransferase
    J C Eads
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Structure 5:47-58. 1997
    ..A conserved sequence motif of 13 residues is common to these 'type I' PRTases but is not observed in the QAPRTase sequence, suggestive of a different fold for this enzyme...
  5. ncbi Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA
    S Noguchi
    J Biol Chem 257:6544-50. 1982
    ..coli for survival in the natural environment...
  6. pmc Transfer of embB codon 306 mutations into clinical Mycobacterium tuberculosis strains alters susceptibility to ethambutol, isoniazid, and rifampin
    Hassan Safi
    Division of Infectious Disease and Center for Emerging Pathogens, Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    Antimicrob Agents Chemother 52:2027-34. 2008
    ..The unusual growth property of embB306 mutants in other antibiotics suggests that they may be amplified during treatment in humans and that a single mutation may affect antibiotic susceptibility against multiple first-line antibiotics...
  7. pmc The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol
    A E Belanger
    Department of Microbiology, Colorado State University, Fort Collins 80523, USA
    Proc Natl Acad Sci U S A 93:11919-24. 1996
    ....
  8. ncbi Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination
    Alexander G Maier
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    Mol Biochem Parasitol 150:118-21. 2006
  9. ncbi In vivo cancer gene therapy by adenovirus-mediated transfer of a bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion gene
    P Erbs
    Transgene S A Strasbourg, France
    Cancer Res 60:3813-22. 2000
    ..These unique properties make of the FCU1/5-FC prodrug activation system a novel and powerful candidate for cancer gene therapy strategies...
  10. pmc Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo
    Kirti Sharma
    Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India
    J Bacteriol 188:2936-44. 2006
    ..These findings and in vivo upregulation of PknH inside the host macrophages suggest a functionally relevant signaling mechanism involving the PknH-EmbR-embCAB system...
  11. ncbi Elevated xylosyltransferase I activities in pseudoxanthoma elasticum (PXE) patients as a marker of stimulated proteoglycan biosynthesis
    Christian Gotting
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany
    J Mol Med (Berl) 83:984-92. 2005
    ..Serum XT-I, the novel fibrosis marker, may be useful for the assessment of extracellular matrix alterations and disease activity in PXE...
  12. ncbi Pyrimidine salvage pathway in Mycobacterium tuberculosis
    A D Villela
    Instituto Nacional de Ciência e Tecnologia em Tuberculose, Centro de Pesquisas em Biologia Molecular e Funcional CPBMF, Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Curr Med Chem 18:1286-98. 2011
    ..Enzyme functional and structural data have been included to provide a broader knowledge on which to base the search for compounds with selective biological activity...
  13. pmc Development and application of a positive-negative selectable marker system for use in reverse genetics in Plasmodium
    Joanna A M Braks
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, The Netherlands
    Nucleic Acids Res 34:e39. 2006
    ..This is the first demonstration of the general use of yFCU in combination with a positive selectable marker in reverse genetics approaches and it should be possible to adapt its use to many other biological systems...
  14. ncbi Crystal structure of quinolinic acid phosphoribosyltransferase from Helicobacter pylori
    Mun Kyoung Kim
    Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, South Korea
    Proteins 63:252-5. 2006
  15. ncbi Purine Salvage Pathway in Mycobacterium tuberculosis
    R G Ducati
    Instituto Nacional de Ciência e Tecnologia em Tuberculose INCT TB, Centro de Pesquisas em Biologia Molecular e Funcional CPBMF, Pontificia Universidade Catolica do Rio Grande do Sul PUCRS, Porto Alegre, RS, Brazil
    Curr Med Chem 18:1258-75. 2011
    ..We have focused on the mycobacterial homologues of this pathway as potential targets for the development of new antitubercular agents...
  16. pmc Allelic exchange and mutant selection demonstrate that common clinical embCAB gene mutations only modestly increase resistance to ethambutol in Mycobacterium tuberculosis
    Hassan Safi
    Division of Infectious Disease and the Center for Emerging Pathogens, Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    Antimicrob Agents Chemother 54:103-8. 2010
    ..tuberculosis strains with identical mutations suggest that clinical EMB resistance is multigenic and that high-level EMB resistance requires mutations in currently unknown loci...
  17. pmc Mycobacterium tuberculosis embB codon 306 mutations confer moderately increased resistance to ethambutol in vitro and in vivo
    Claudia Plinke
    Molecular Mycobacteriology Group, National Reference Center for Mycobacteria, Forschungszentrum Borstel, Parkallee 1 40, 23845 Borstel, Germany
    Antimicrob Agents Chemother 55:2891-6. 2011
    ..We propose that conventional EMB susceptibility testing, in combination with embB306 genotyping, may guide dose adjustment to avoid clinical treatment failure in these low-level resistant strains...
  18. pmc Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase
    Hideyuki Takeuchi
    Department of Biochemistry and Cell Biology, Institute of Cell and Developmental Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 108:16600-5. 2011
    ....
  19. pmc Site-specific modification of Shigella flexneri virF mRNA by tRNA-guanine transglycosylase in vitro
    Julie K Hurt
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, USA
    Nucleic Acids Res 35:4905-13. 2007
    ....
  20. ncbi High-affinity inhibitors of tRNA-guanine transglycosylase replacing the function of a structural water cluster
    Philipp C Kohler
    Laboratorium fur Organische Chemie, ETH Zurich, Honggerberg, HCI, 8093 Zurich, Switzerland
    Chemistry 15:10809-17. 2009
    ..In addition, a synthetic intermediate with an unusual 3,6,7,8,9,10-hexahydroimidazo[4,5-g][1,3]benzodiazepine core, as confirmed by X-ray analysis, is reported...
  21. ncbi Crystal structure of quinolinic acid phosphoribosyltransferase from Mmycobacterium tuberculosis: a potential TB drug target
    V Sharma
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Structure 6:1587-99. 1998
    ..Quinolinic acid phosphoribosyltransferase (QAPRTase), a key enzyme in the de novo biosynthesis of NAD, provides an attractive target for designing novel antitubercular drugs...
  22. pmc Polymorphisms in the xylosyltransferase genes cause higher serum XT-I activity in patients with pseudoxanthoma elasticum (PXE) and are involved in a severe disease course
    S Schon
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    J Med Genet 43:745-9. 2006
    ..Therefore, we propose the XYLT genes encoding xylosyltransferase I (XT-I) as the chain-initiating enzyme in the biosynthesis of proteoglycans and the highly homologous XT-II as potential candidate genes...
  23. pmc Structural and kinetic characterization of quinolinate phosphoribosyltransferase (hQPRTase) from homo sapiens
    Huanting Liu
    The Centre for Biomolecular Science, The University of St Andrews, North Haugh, St Andrews KY16 9ST, Scotland
    J Mol Biol 373:755-63. 2007
    ..The human enzyme adopts a hexameric arrangement, which places the active sites in close proximity to each other...
  24. pmc Expression of the bifunctional suicide gene CDUPRT increases radiosensitization and bystander effect of 5-FC in prostate cancer cells
    Ligang Xing
    Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Radiother Oncol 92:345-52. 2009
    ....
  25. pmc Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure
    Yi Chen Chen
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    RNA 16:958-68. 2010
    ..hQTRTD1 has been implicated as the salvage enzyme that generates free queuine from QMP. Work is ongoing in our laboratory to confirm this activity...
  26. ncbi Combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice
    Aparajita Khatri
    Oncology Research Centre, Prince of Wales Hospital Clinical School of Medicine, The University of New South Wales, Randwick, NSW 2031, Australia
    J Gene Med 8:1086-96. 2006
    ..The product of the fusion gene, CDUPRT, converts the prodrug, 5-fluorocytosine (5FC), into 5-fluorouracil (5FU) and other cytotoxic metabolites that kill both CDUPRT-expressing and surrounding cells, via a 'bystander effect'...
  27. pmc The critical role of embC in Mycobacterium tuberculosis
    Renan Goude
    Centre for Infectious Disease, Barts and The London, Queen Mary s School of Medicine and Dentistry, London E1 2AT, United Kingdom
    J Bacteriol 190:4335-41. 2008
    ..In conclusion, we have identified one of the key genes involved in LAM biosynthesis in M. tuberculosis and confirmed its essential role in this species...
  28. ncbi Association between embB mutations and ethambutol resistance in Mycobacterium tuberculosis isolates from Cuba and the Dominican Republic: reproducible patterns and problems
    Elba Guerrero
    Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Rev Argent Microbiol 45:21-6. 2013
    ..One ethambutol resistant non-MDR strain did not have any embB mutation suggesting that other undefined mutations can also confer ethambutol resistance...
  29. pmc Mg2+ binding and archaeosine modification stabilize the G15 C48 Levitt base pair in tRNAs
    Romina Oliva
    Dipartimento di Scienze Applicate, Università di Napoli Parthenope, Naples, Italy
    RNA 13:1427-36. 2007
    ..Metal binding and chemical modification thus play an interchangeable role in stabilizing the G15-C48 correct geometry. Interestingly, these different but convergent strategies are selectively adopted in the different life domains...
  30. pmc Characteristics of embB mutations in multidrug-resistant Mycobacterium tuberculosis isolates in Henan, China
    Dawei Shi
    State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China
    J Antimicrob Chemother 66:2240-7. 2011
    ..To determine the association between embB mutations and drug resistance, and to further investigate the mechanism of embB mutations involved in the development of ethambutol and multidrug resistance in Mycobacterium tuberculosis...
  31. pmc Queuosine formation in eukaryotic tRNA occurs via a mitochondria-localized heteromeric transglycosylase
    Coilin Boland
    School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
    J Biol Chem 284:18218-27. 2009
    ..Confocal and immunoblot analysis suggest that TGT weakly interacts with the outer mitochondrial membrane possibly through association with Qv1, which was found to be stably associated with the organelle...
  32. ncbi Possible participation of oxidative stress in causation of cell proliferation and in vivo mutagenicity in kidneys of gpt delta rats treated with potassium bromate
    Takashi Umemura
    Division of Pathology, National Institute of Health Sciences, 1 18 1, Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Toxicology 257:46-52. 2009
    ..The overall data indicated that while oxidative stress well correlates with induction of cell proliferation in females, its role in males and in generation of in vivo mutagenicity by KBrO(3) in both sexes is limited...
  33. pmc Identification of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats
    Maya K Sethi
    Department of Cellular Chemistry, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    J Biol Chem 285:1582-6. 2010
    ....
  34. pmc Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations
    S Sreevatsan
    Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA
    Antimicrob Agents Chemother 41:1677-81. 1997
    ..The data are consistent with the idea that amino acid substitutions in EmbB alter the drug-protein interaction and thereby cause EMB resistance...
  35. pmc Arabidopsis irregular xylem8 and irregular xylem9: implications for the complexity of glucuronoxylan biosynthesis
    Maria J Peña
    Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 19:549-63. 2007
    ..Our results suggest that IRX9 is required for normal GX elongation and indicate roles for IRX8 and FRA8 in the synthesis of the glycosyl sequence at the GX reducing end...
  36. ncbi Correlation of the phenotypic ethambutol susceptibility of Mycobacterium tuberculosis with embB gene mutations in Korea
    Young Kil Park
    Korean Institute of Tuberculosis, 482 Mansuri, Kangwoimyun, Cheongwongun, Chungbuk, Republic of Korea
    J Med Microbiol 61:529-34. 2012
    ..0052). Three novel embB mutations at codons 399, 405 and 459 were identified in this study. These results support the hypothesis that embB mutation except for a few specific mutation types may be the main cause of EMB resistance...
  37. pmc Non-invasive molecular and functional imaging of cytosine deaminase and uracil phosphoribosyltransferase fused with red fluorescence protein
    Ligang Xing
    Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York 10065, USA
    Acta Oncol 47:1211-20. 2008
    ..Thus, we developed a preclinical tumor model to investigate the feasibility of using magnetic resonance spectroscopy and optical imaging to measure non-invasively CD and UPRT expression and function...
  38. ncbi The use of 19F spectroscopy and diffusion-weighted MRI to evaluate differences in gene-dependent enzyme prodrug therapies
    Daniel A Hamstra
    Department of Radiation Oncology, The University of Michigan Medical Center, Ann Arbor 48109 0582, USA
    Mol Ther 10:916-28. 2004
    ....
  39. ncbi Mutations in the embB locus among Korean clinical isolates of Mycobacterium tuberculosis resistant to ethambutol
    Hye Young Lee
    Institute of Immunology and Immunological Diseases, Yonsei University College of Medicine, C P O Box 8044, Seoul 120 752, Korea
    Yonsei Med J 43:59-64. 2002
    ..On the other hand, all of the EMB-susceptible isolates were found to be free of mutations. In conclusion, our findings suggest that sequence polymorphism of embB may play a pivotal role in the EMB- resistance of M. tuberculosis...
  40. ncbi Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit
    K L Deshpande
    Department of Molecular Sciences, The University of Tennessee Health Science Center, 858 Madison Avenue, 38163, Memphis, TN, USA
    Gene 265:205-12. 2001
    ..AC067805, bp 148976-149683); the 3'-end, bp 726-1390, is identical to the 3'-end of the TGT cDNA sequence from IMAGE:611146...
  41. ncbi Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    Chembiochem 4:1066-77. 2003
    ..In consequence, full understanding of this plasticity can be exploited for drug design...
  42. ncbi Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT
    Tina Ritschel
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, Marburg, Germany
    Chembiochem 10:716-27. 2009
    ..Docking suggests multiple orientations of these side chains. Obviously, an entropic advantage of the residual mobility experienced by these ligands in the bound state is beneficial and reveals an overall improved protein binding...
  43. ncbi Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity
    U Grädler
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Germany
    FEBS Lett 454:142-6. 1999
    ..The crystal structure of a TGT(S103A)/preQ1 complex combined with biochemical data presented in this paper suggest that Ser103 is essential for substrate orientation in the TGT reaction...
  44. ncbi tRNA-guanine transglycosylase from Escherichia coli: recognition of full-length 'queuine-cognate' tRNAs
    F L Kung
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    FEBS Lett 431:427-32. 1998
    ..Interestingly, the yeast tRNAs are essentially equivalent to the E. coli tRNAs as substrates for TGT, indicating that there is nothing intrinsic to the yeast tRNAs that accounts for the absence of queuine in yeast...
  45. ncbi Purification, crystallization, and preliminary x-ray diffraction studies of tRNA-guanine transglycosylase from Zymomonas mobilis
    C Romier
    European Molecular Biology Laboratory, Structural Biology Programme, Heidelberg, Germany
    Proteins 24:516-9. 1996
    ..1 A, b = 65.1 A, c = 71.9 A, and beta = 97.5 degrees and contain one molecule per asymmetric unit. A complete diffraction data set from one native crystal has been obtained at 1.85 A resolution...
  46. pmc Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies
    Naomi Tidten
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 374:764-76. 2007
    ..The way this is achieved, however, significantly differs from that predicted based on crystal structures of wild-type Tgt...
  47. pmc Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases
    Yi Chen Chen
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, USA
    Nucleic Acids Res 39:2834-44. 2011
    ..Both the phylogenetic and kinetic analyses support the conclusion that all TGTs have divergently evolved to specifically recognize their cognate heterocyclic substrates...
  48. ncbi A UGU sequence in the anticodon loop is a minimum requirement for recognition by Escherichia coli tRNA-guanine transglycosylase
    S Nakanishi
    Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo, Japan
    J Biol Chem 269:32221-5. 1994
    ..Our results suggest that the enzyme recognizes the U33-G34-U35 sequence in the anti-codon loop and not the tertiary structure of tRNA itself...
  49. pmc Queuosine deficiency in eukaryotes compromises tyrosine production through increased tetrahydrobiopterin oxidation
    Tatsiana Rakovich
    School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    J Biol Chem 286:19354-63. 2011
    ..Our data suggest that queuosine modification limits BH4 oxidation in vivo and thereby potentially impacts on numerous physiological processes in eukaryotes...
  50. pmc Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases
    C Eric Thomas
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, United States
    Biochem Biophys Res Commun 410:34-9. 2011
    ..The pteridine inhibition results are consistent with an earlier hypothesis that pteridines may regulate eukaryal TGT activity (Jacobson et al., Nuc. Acids Res. 9 (1981) 2351 [8])...
  51. ncbi A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase
    U Grädler
    Institut fur Pharmazeutische Chemie, Marbacher Weg 6, Philipps Universitat Marburg, 35032, Germany
    J Mol Biol 306:455-67. 2001
    ..The 1.95 A crystal structure of APH in complex with Z. mobilis TGT served as a starting point for further modification of this initial lead...
  52. ncbi How to replace the residual solvation shell of polar active site residues to achieve nanomolar inhibition of tRNA-guanine transglycosylase
    Tina Ritschel
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    ChemMedChem 4:2012-23. 2009
    ..Furthermore, conformational preferences of attached hydrophobic moieties explain their contribution to a gradual increase in binding affinity...
  53. pmc The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase
    K T Hughes
    Department of Microbiology, University of Washington, Seattle 98195
    J Bacteriol 175:479-86. 1993
    ..Steady-state kinetics on the purified QAPRTase indicated Km values of 32 microM for 5-phosphoribosyl-1-pyrophosphate and 20 microM for quinolinate. Vmax was 0.9 U/mg, similar to values reported for this enzyme by other sources...
  54. ncbi Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12
    U B Rasmussen
    Biochim Biophys Acta 881:268-75. 1986
    ..The effect of GTP was found to be pH-dependent. The enzymatic characterization of uracil phosphoribosyltransferase and the observed regulation of its synthesis emphasizes the role of the enzyme in pyrimidine salvage...
  55. ncbi tRNA-guanine transglycosylase from Escherichia coli. Minimal tRNA structure and sequence requirements for recognition
    A W Curnow
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    J Biol Chem 270:17264-7. 1995
    ..Ueda, T., Hori, H., Yamazaki, N., Okada, N., and Watanabe, K. (1994) J. Biol. Chem. 269, 32221-32225) indicating that a UGU sequence in a 7-base loop is the minimal requirement for TGT recognition...
  56. ncbi Recombinant uracil phosphoribosyltransferase from the thermophile Bacillus caldolyticus: expression, purification, and partial characterization
    H K Jensen
    Center for Enzyme Research, University of Copenhagen, Denmark
    Protein Expr Purif 10:356-64. 1997
    ..By comparing the UPRT activity of crude extracts of B. subtilis and B. caldolyticus it was found that the enzyme from B. caldolyticus was considerably more stable toward thermal inactivation than the homologous enzyme from B. subtilis...
  57. ncbi Crystal structure of a type II quinolic acid phosphoribosyltransferase (TM1645) from Thermotoga maritima at 2.50 A resolution
    Robert Schwarzenbacher
    The Joint Center for Structural Genomics, The San Diego Supercomputer Center, La Jolla, California 92093, USA
    Proteins 55:768-71. 2004
  58. ncbi Transforming growth factor beta1-regulated xylosyltransferase I activity in human cardiac fibroblasts and its impact for myocardial remodeling
    Christian Prante
    Institut für Laboratoriumsund Transfusionsmedizin, Herzund Diabeteszentrum NRW, Universitatsklinik der Ruhr Universitat Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany
    J Biol Chem 282:26441-9. 2007
    ..Specific blocking of XT-I expression confirmed that XT-I catalyzes a rate-limiting step during fibrotic GAG biosynthesis...
  59. pmc The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97201 3098, USA
    Proc Natl Acad Sci U S A 99:78-83. 2002
    ..Thus, our studies reveal an allosteric mechanism involving nucleotide stabilization of a more active, higher order oligomer. Such regulation of UPRT could play a role in the balance of purine and pyrimidine nucleotide pools in the cell...
  60. ncbi Modulation of xylosyltransferase I expression provides a mechanism regulating glycosaminoglycan chain synthesis during cartilage destruction and repair
    Narayanan Venkatesan
    UMR 7561 CNRS Université Henri Poincaré Nancy 1, Faculte de Medecine, BP 184, 54505 Vandoeuvre les Nancy, France
    FASEB J 23:813-22. 2009
    ..These findings show that GTs play a key role in the loss of PG-GAGs in joint diseases and identify novel targets for stimulating cartilage repair...
  61. ncbi Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus
    Kaj F Jensen
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Denmark
    FEBS J 272:1440-53. 2005
    ..The data indicate that UPRTase undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and PRPP...
  62. ncbi The evolution of catalytic residues and enzyme mechanism within the bacterial nucleoside phosphorylase superfamily 1
    Anke Konrad
    Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA
    Gene 510:154-61. 2012
    ..This study provides a basis for understanding the evolution of uridine and purine nucleoside phosphorylases with respect to DNA/RNA metabolism and with potential utility in the design of antimicrobial and anti-tumor drugs...
  63. ncbi Structure of product-bound Bacillus caldolyticus uracil phosphoribosyltransferase confirms ordered sequential substrate binding
    Anders Kadziola
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen, Denmark
    Acta Crystallogr D Biol Crystallogr 58:936-45. 2002
    ..This is consistent with kinetic data, which display ordered sequential binding of substrates, with PRPP binding first. Based on this observation, a reaction mechanism is proposed...
  64. pmc Molecular cloning of a xylosyltransferase that transfers the second xylose to O-glucosylated epidermal growth factor repeats of notch
    Maya K Sethi
    Department of Cellular Chemistry, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    J Biol Chem 287:2739-48. 2012
    ..The enzyme was shown to be a typical type II membrane-bound glycosyltransferase localized in the endoplasmic reticulum...
  65. ncbi Allosteric regulation and communication between subunits in uracil phosphoribosyltransferase from Sulfolobus solfataricus
    Susan Arent
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen Ø, Denmark
    Biochemistry 44:883-92. 2005
    ..The active-site differences have been related to proposed kinetic models and provide an explanation for the regulatory significance of the C-terminal Gly216...
  66. ncbi tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition
    A W Curnow
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065
    Biochemistry 32:5239-46. 1993
    ..A 17-base oligoribonucleotide, corresponding to the anticodon loop and stem, is also a substrate for TGT with only a 20-fold loss in Vmax/KM, versus the full-length tRNA.(ABSTRACT TRUNCATED AT 250 WORDS)..
  67. ncbi Different oligomeric states are involved in the allosteric behavior of uracil phosphoribosyltransferase from Escherichia coli
    K F Jensen
    Center for Enzyme Research, Institute of Molecular Biology, University of Copenhagen, Denmark
    Eur J Biochem 240:637-45. 1996
    ..It appears that the smaller form predominates in the absence of substrates, while the larger form predominates in the presence of GTP and PPRibP. Guanosine-3',5'-bis(diphosphate) was found to activate the enzyme much like GTP...
  68. ncbi Structural and kinetic studies of the allosteric transition in Sulfolobus solfataricus uracil phosphoribosyltransferase: Permanent activation by engineering of the C-terminus
    Stig Christoffersen
    Department of Biology, University of Copenhagen, Biocenter, Copenhagen N, Denmark
    J Mol Biol 393:464-77. 2009
    ..Tagging the C-terminus with additional amino acids generates an endogenously activated enzyme that binds GTP without effects on activity...
  69. ncbi Identification and characterization of human uracil phosphoribosyltransferase (UPRTase)
    Jixi Li
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People s Republic of China
    J Hum Genet 52:415-22. 2007
    ..This means that human UPRTase may have enzymatic activity with another, unknown, factor or have other activity in pyrimidine metabolism...
  70. ncbi Structure of pyrR (Rv1379) from Mycobacterium tuberculosis: a persistence gene and protein drug target
    Katherine A Kantardjieff
    Department of Chemistry and Biochemistry and W M Keck Foundation Center for Molecular Structure, California State University Fullerton, Fullerton, CA 92834, USA
    Acta Crystallogr D Biol Crystallogr 61:355-64. 2005
    ..In silico screening of pyrimidine-nucleoside analogs has revealed a number of potential lead compounds that, if bound to Mtb pyrR, could facilitate transcriptional attenuation, particularly cyclopentenyl nucleosides...
  71. pmc XT-II, the second isoform of human peptide-O-xylosyltransferase, displays enzymatic activity
    Josef Voglmeir
    Department für Chemie, Universität für Bodenkultur, A 1190 Wien, Austria
    J Biol Chem 282:5984-90. 2007
    ..Its pH, temperature, and cation dependences are similar to those of XT-I expressed in either mammalian cells or yeast. Our data suggest that XT-I and XT-II are, at least in vitro, functionally identical...
  72. ncbi Comparative characterisation of recombinant invertebrate and vertebrate peptide O-Xylosyltransferases
    Andrea Brunner
    Department für Chemie der, Universität für Bodenkultur, Muthgasse 18, A 1190, Wien, Austria
    Glycoconj J 23:543-54. 2006
    ..Using this substrate, we could for the first time follow, by mass spectrometry, the xylosylation of a peptide with multiple xylosyltransferase acceptor motifs...
  73. ncbi Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae
    S U Aström
    Department of Microbiology, Umea University, Sweden
    Mol Gen Genet 261:967-76. 1999
    ....
  74. ncbi Recognition of acceptor proteins by UDP-D-xylose proteoglycan core protein beta-D-xylosyltransferase
    T Brinkmann
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany
    J Biol Chem 272:11171-5. 1997
    ..These results imply that the primary structure of the acceptor is not the only determinant for recognition by xylosyltransferase. Thus, protein conformation is also a main factor in determining xylosylation...
  75. ncbi Functional characterization of Drosophila melanogaster peptide O-xylosyltransferase, the key enzyme for proteoglycan chain initiation and member of the core 2/I N-acetylglucosaminyltransferase family
    Iain B H Wilson
    Glycobiology Division, Institut fur Chemie, Universität für Bodenkultur, Muthgasse 18, A 1190 Wien, Austria
    J Biol Chem 277:21207-12. 2002
    ..Because in contrast to mammals, there is only one fly peptide O-xylosyltransferase gene, it is anticipated that, given the key roles of proteoglycans, the hereby designated oxt gene is essential for viability...
  76. ncbi tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands
    S Chong
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065
    Biochemistry 34:3694-701. 1995
    ..These results also suggest that the zinc site is necessary for TGT homotrimer formation and for tRNA binding...
  77. ncbi Crystal structures of tRNA-guanine transglycosylase (TGT) in complex with novel and potent inhibitors unravel pronounced induced-fit adaptations and suggest dimer formation upon substrate binding
    Bernhard Stengl
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 370:492-511. 2007
    ..It is hypothesized that one unit of the dimer performs the catalytic reaction whereas the second is required to recognize and properly orient the bound tRNA for the catalytic reaction...
  78. ncbi Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination
    S U Aström
    Department of Microbiology, University of Umea, Sweden
    Cell 79:535-46. 1994
    ..The modification enzyme was shown to recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs...
  79. ncbi Cloning and recombinant expression of active full-length xylosyltransferase I (XT-I) and characterization of subcellular localization of XT-I and XT-II
    Sylvia Schön
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, 32545 Bad Oeynhausen, Germany
    J Biol Chem 281:14224-31. 2006
    ..Unlike XT-I, for XT-II, the first 45 amino acids are sufficient to target and retain the GFP reporter in the Golgi compartment. Here we show evidence that the stem regions were indispensable for Golgi localization of XT-I and XT-II...
  80. ncbi Concomitant expression of E. coli cytosine deaminase and uracil phosphoribosyltransferase improves the cytotoxicity of 5-fluorocytosine
    M Tiraby
    CAYLA, Toulouse, France
    FEMS Microbiol Lett 167:41-9. 1998
    ....
  81. pmc Arabinoxylan biosynthesis in wheat. Characterization of arabinosyltransferase activity in Golgi membranes
    Andrea Celia Porchia
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 130:432-41. 2002
    ..The [(14)C]Ara labeling became reversible by adding unlabeled UDP-Xyl to the reaction medium. The possible role of this protein in arabinoxylan biosynthesis is discussed...
  82. ncbi Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate
    Wei Xie
    Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Nat Struct Biol 10:781-8. 2003
    ..Furthermore, reaction of PreQ1 with the crystallized covalent intermediate provides insight into the necessary structural changes required for the TGT-catalyzed reaction to occur...
  83. ncbi Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity
    Christian Gotting
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany
    J Biol Chem 279:42566-73. 2004
    ..9-24.6 microm for the XT-I mutants. The properties of binding of XT-I to heparin-beads, the Ki constants for noncompetitive inhibition by heparin, and the activation by protamine were not altered by the generated mutations...
  84. pmc New insight into intrachromosomal deletions induced by chrysotile in the gpt delta transgenic mutation assay
    An Xu
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Environ Health Perspect 115:87-92. 2007
    ..However, there is less evidence to demonstrate the contribution of deletions to the mutagenicity of asbestos fibers in vivo...
  85. ncbi Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay
    Michael D Cleary
    Nat Biotechnol 23:232-7. 2005
    ..gondii UPRT, indicating that engineered UPRT expression will allow cell-specific analysis of gene expression in organisms other than T. gondii...
  86. ncbi The xylosyltransferase I gene polymorphism c.343G>T (p.A125S) is a risk factor for diabetic nephropathy in type 1 diabetes
    Sylvia Schön
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, 32545 Bad Oeynhausen, Germany
    Diabetes Care 29:2295-9. 2006
    ..Thus, genetic variations in the xylosyltransferase genes might be implicated in the initiation and progression of these complications...
  87. pmc Human xylosyltransferase I and N-terminal truncated forms: functional characterization of the core enzyme
    Sandra Muller
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany
    Biochem J 394:163-71. 2006
    ..Our study demonstrates that over 80% of the nucleotide sequence of the XT-I-cDNA is necessary for expressing a recombinant enzyme with full catalytic activity...
  88. pmc Role of aspartate 143 in Escherichia coli tRNA-guanine transglycosylase: alteration of heterocyclic substrate specificity
    Katherine Abold Todorov
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 45:617-25. 2006
    ..This confirms the key role of aspartate 143 in maintaining the anticodon identities of the queuine-containing tRNAs and suggests that TGT mutants could be developed that would alter the tRNA wobble base base pairing properties...
  89. ncbi The formation of extracellular matrix during chondrogenic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I
    Christian Prante
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum NRW, Universitatsklinik der Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    Stem Cells 24:2252-61. 2006
    ..In conclusion, we could show that key enzymes for CS, DS, and HS synthesis, especially XT-I, are useful markers for the developmental stages of chondrogenic differentiation...
  90. ncbi embCAB sequence variation among ethambutol-resistant Mycobacterium tuberculosis isolates without embB306 mutation
    Claudia Plinke
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
    J Antimicrob Chemother 65:1359-67. 2010
    ..Here, other mutations in the embCAB operon are suggested to be involved in resistance development...
  91. ncbi Kinetic mechanism of uracil phosphoribosyltransferase from Escherichia coli and catalytic importance of the conserved proline in the PRPP binding site
    C Lundegaard
    Center for Enzyme Research, Institute of Molecular Biology, University of Copenhagen, Denmark
    Biochemistry 38:3327-34. 1999
    ..We conclude that the proline in the PRPP binding site of UPRTase is of only little importance for binding of PRPP to the free enzyme, but is critical for binding of uracil to the enzyme-PRPP complex and for the catalytic rate...
  92. pmc Identification of the rate-determining step of tRNA-guanine transglycosylase from Escherichia coli
    George A Garcia
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 48:11243-51. 2009
    ....
  93. pmc Detection of embB306 mutations in ethambutol-susceptible clinical isolates of Mycobacterium tuberculosis from Northwestern Russia: implications for genotypic resistance testing
    Igor Mokrousov
    Laboratory of Molecular Microbiology, St Petersburg Pasteur Institute, 197101 St Petersburg, Russia
    J Clin Microbiol 40:3810-3. 2002
    ..Comparison with DNA fingerprinting data showed that, irrespectively of the phenotypic susceptibility profiles, 46 (50.6%) of 91 Beijing family strains and 16 (17.4%) of 92 strains of other genotypes had a mutation in embB306...
  94. pmc Mutations at embB codon 306 are an important molecular indicator of ethambutol resistance in Mycobacterium tuberculosis
    Angela M Starks
    Division of Tuberculosis Elimination, National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Antimicrob Agents Chemother 53:1061-6. 2009
    ..Our data indicate that embB 306 mutations are sufficient to confer ethambutol resistance, and detection of these mutations should be considered in the development of rapid molecular tests...
  95. ncbi RNA analysis by biosynthetic tagging using 4-thiouracil and uracil phosphoribosyltransferase
    Gusti M Zeiner
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA
    Methods Mol Biol 419:135-46. 2008
    ..This chapter updates the original RABT protocol (1) and addresses methodological details associated with RABT that were beyond the scope or space allotment of the initial report...
  96. ncbi tRNA-guanine transglycosylase from Escherichia coli: recognition of dimeric, unmodified tRNA(Tyr)
    A W Curnow
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    Biochimie 76:1183-91. 1994
    ..Here we find that our earlier conclusion, that the recognition elements for TGT are localized within the anticodon arm of cognate tRNAs, is supported...
  97. ncbi Topology and mutational analysis of the single Emb arabinofuranosyltransferase of Corynebacterium glutamicum as a model of Emb proteins of Mycobacterium tuberculosis
    Mathias Seidel
    Institute for Biotechnology 1, Research Centre Juelich, D 52425 Juelich, Germany
    Glycobiology 17:210-9. 2007
    ..Taken together, the data clearly define important residues of Emb involved in arabinan domain formation and, for the first time, shed new light on the topology of this important enzyme...
  98. pmc Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan
    Ai Min Wu
    School of Biological Sciences, University of Southampton, Boldrewood Campus, Southampton SO16 7PX, United Kingdom
    Plant Physiol 153:542-54. 2010
    ..Our findings reveal two distinct sets of four genes each differentially contributing to GX biosynthesis...
  99. pmc The purine salvage enzyme hypoxanthine guanine xanthine phosphoribosyl transferase is a major target antigen for cell-mediated immunity to malaria
    Morris O Makobongo
    Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane 4029, Australia
    Proc Natl Acad Sci U S A 100:2628-33. 2003
    ..Recombinant HGXPRT from the human malaria parasite, Plasmodium falciparum, activated the T cells in vitro, and immunization of normal mice with recombinant HGXPRT reduced parasite growth rates in all mice after challenge...
  100. ncbi Measurement of fibrosis marker xylosyltransferase I activity by HPLC electrospray ionization tandem mass spectrometry
    Joachim Kuhn
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    Clin Chem 52:2243-9. 2006
    ..Therefore, the measurement of serum XT-I activity is useful to monitor disease activity in these patients...
  101. pmc tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition
    S T Nonekowski
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    RNA 7:1432-41. 2001
    ..The fact that the other analogs were not active indicates that although TGT is capable of recognizing the U-G-U sequence in additional contexts, this recognition is not indiscriminate...

Research Grants20

  1. THE MUTAGENICITY OF MINERAL FIBERS
    TOM HEI; Fiscal Year: 2006
    ..By using specific DNA probes of other genes that have been regionally mapped to various sites on chromosome 11, the molecular spectrum of mutations induced by either asbestos or glass fiber control will be compared. ..
  2. MECHANISMS OF FIBER CARCINOGENESIS
    TOM HEI; Fiscal Year: 2001
    ..Cytogenetic and molecular alterations at the gene level will be analyzed from tumors and intermediate transformation stages to provide a mechanistic basis for tumor induction by mineral fibers. ..
  3. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
    ..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
  4. GENE DISCOVERY IN TOXOPLASMA GONDII
    L Sibley; Fiscal Year: 2003
    ..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
  5. Development of New GI-specific Histone Deacetylase Inhi*
    Scott Rajski; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  6. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
    ..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
  7. Mucosal Immunity to Toxoplasma gondii
    L Sibley; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  8. MECHANISMS OF ARSENIC CARCINOGENESIS
    TOM HEI; Fiscal Year: 2005
    ..abstract_text> ..
  9. Structural Biology of RNA
    ADRIAN FERRE D AMARE; Fiscal Year: 2009
    ..These 'molecular movies'will provide the basic understanding that will help manipulate the functions of RNAs in human health and disease, and in the life cycle of pathogenic organisms. ..
  10. Molecular dynamics studies of muscle proteins
    EDWARD F PATE; Fiscal Year: 2010
    ..Improved understanding of the function of myosin V and myosin VI can be anticipated to improve therapies for Griscelli syndrome and myosin Vl-based sensorineural hearing loss in humans. ..
  11. Substrate Recognition of a tRNA Modifying Enzyme
    George Garcia; Fiscal Year: 2006
    ..A clear understanding of the similarities and differences between the TGTs from eubacteria and human will be of significance for many reasons but most obviously for the future design of novel antibiotics. ..
  12. MSC BLUE-3 AND MSC PURPLE-3 CONFOCAL X-RAY OPTICS
    ADRIAN FERRE D AMARE; Fiscal Year: 2001
    ..We are requesting funds to purchase MSC/Max-Flux confocal optics that will result in up to 27-fold increase in useable X-ray flux, relative to the current configuration. ..
  13. Virulence genes identification in Francisella tularensis
    Vincent Escuyer; Fiscal Year: 2004
    ..tularensis and a better understanding of the pathogenicity of this microorganism. Some of the virulence genes will be used as potential targets for new approaches in drug design and vaccine development. ..
  14. ANALYSIS OF MOTOR PROTEIN FUNCTION
    Edward Pate; Fiscal Year: 2004
    ..Although this approach will be shown to be a two-way street, the most significant advantage clearly accrues to the much less studied microtubule motor family. ..
  15. ANALYSIS OF MOTOR PROTEIN FUNCTION
    Edward Pate; Fiscal Year: 2009
    ..Together these data will lead to a more detailed picture of how conformational changes at the nucleotide site are involved in force generation. ..
  16. Towards drugs that prevent resistance to the HIV OI, TB
    David Alland; Fiscal Year: 2006
    ..if embB306 mutations affect biosynthesis of LAM, capped LAM, arabinogalactan (AG) or other cell wall components in the presence and absence of EMB. ..
  17. TUBERCULOSIS GENOTYPING AND EVOLUTIONARY CONSORTIUM
    David Alland; Fiscal Year: 2005
    ..4) To identify and validate an informative set of SNPs for worldwide evolutionary and epidemiological analyses. ..
  18. Detection of Select Agents in Single-Well Assays
    David Alland; Fiscal Year: 2007
    ..3. To develop PCR assays that utilize "color-triplet coding" to uniquely label as many as 56 different species-specific molecular beacons. ..
  19. Sample Processing Cartridges for Rapid PCR TB Detection
    David Alland; Fiscal Year: 2008
    ..The system will then be retested using a large number of clinical sputum specimens. Finally, we will develop complete documentation on the assay including production, and development reports. [unreadable] [unreadable] [unreadable]..
  20. POPULATION BASED INVESTIGATIONS OF TUBERCULOSIS
    David Alland; Fiscal Year: 2004
    ..Studies of these sequence variations in clinical strains will be used to determine the associations between specific mutations and the phenotypes of infectivity and virulence. ..