n acetylglucosaminyltransferases

Summary

Summary: Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.

Top Publications

  1. ncbi A combined analytical approach reveals novel EXT1/2 gene mutations in a large cohort of Italian multiple osteochondromas patients
    Emanuela Signori
    Laboratory of Molecular Medicine and Biotechnology, University Campus Bio Medico School of Medicine and Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy
    Genes Chromosomes Cancer 46:470-7. 2007
  2. ncbi The EXT2 multiple exostoses gene defines a family of putative tumour suppressor genes
    D Stickens
    McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas 75235 8591, USA
    Nat Genet 14:25-32. 1996
  3. ncbi Aberrant N-glycosylation of beta1 integrin causes reduced alpha5beta1 integrin clustering and stimulates cell migration
    Hua Bei Guo
    Department of Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA
    Cancer Res 62:6837-45. 2002
  4. pmc Molecular basis of N-acetylglucosaminyltransferase I deficiency in Arabidopsis thaliana plants lacking complex N-glycans
    Richard Strasser
    Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Institut für Angewandte Genetik und Zellbiologie, Universitat fur Bodenkultur Wien, Muthgasse 18, A 1190 Wien, Austria
    Biochem J 387:385-91. 2005
  5. pmc Multiple osteochondromas
    Judith V M G Bovee
    Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
    Orphanet J Rare Dis 3:3. 2008
  6. pmc Genotype-phenotype correlation in hereditary multiple exostoses
    C Francannet
    Service de Pédiatrie B et de Génétique, Hotel Dieu, BP 69, Clermont Ferrand, France
    J Med Genet 38:430-4. 2001
  7. pmc Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates
    E Ioffe
    Department of Cell Biology, Albert Einstein College of Medicine, New York, NY 10461
    Proc Natl Acad Sci U S A 91:728-32. 1994
  8. pmc The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate
    C McCormick
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Proc Natl Acad Sci U S A 97:668-73. 2000
  9. ncbi Branched N-glycans regulate the biological functions of integrins and cadherins
    Yanyang Zhao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    FEBS J 275:1939-48. 2008
  10. ncbi Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis
    K Murata
    Department of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari, Japan
    Clin Cancer Res 6:1772-7. 2000

Research Grants

  1. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
  2. O-Glycosylation of Epidermal Growth Factor-like Motifs
    Robert S Haltiwanger; Fiscal Year: 2013
  3. GLYCOSYLTRANSFERASE GENE EXPRESSION IN TRANSFORMED CELLS
    Nevis Fregien; Fiscal Year: 1992
  4. GLYCOPROTEINS IN DIFFERENTIATION AND ONCOGENESIS
    Minoru Fukuda; Fiscal Year: 2009
  5. Polylactosamines in Development and Cancer
    Minoru Fukuda; Fiscal Year: 2007
  6. NEURONAL MIGRATION IN THE DEVELOPING BRAIN
    Huaiyu Hu; Fiscal Year: 2003
  7. Molecular Studies of Brain Malformations
    Huaiyu Hu; Fiscal Year: 2007
  8. Genetic Studies of Clubfoot (ITEV)
    Jacqueline T Hecht; Fiscal Year: 2010
  9. Strategy for Therapy of the Pseudoachondroplasia (PSACH) Phenotype: Understanding
    Jacqueline Hecht; Fiscal Year: 2006
  10. O-GlcNAc:Developing New Tool for Assessment of Glycemia
    Gerald Hart; Fiscal Year: 2008

Detail Information

Publications231 found, 100 shown here

  1. ncbi A combined analytical approach reveals novel EXT1/2 gene mutations in a large cohort of Italian multiple osteochondromas patients
    Emanuela Signori
    Laboratory of Molecular Medicine and Biotechnology, University Campus Bio Medico School of Medicine and Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy
    Genes Chromosomes Cancer 46:470-7. 2007
    ..More important, we were able to characterize all those patients who were negative at the first PCR-based method screening...
  2. ncbi The EXT2 multiple exostoses gene defines a family of putative tumour suppressor genes
    D Stickens
    McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas 75235 8591, USA
    Nat Genet 14:25-32. 1996
    ..Both EXT1 and EXT2 show significant homology with one additional expressed sequence tag, defining a new multigene family of proteins with potential tumour suppressor activity...
  3. ncbi Aberrant N-glycosylation of beta1 integrin causes reduced alpha5beta1 integrin clustering and stimulates cell migration
    Hua Bei Guo
    Department of Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA
    Cancer Res 62:6837-45. 2002
    ..These effects most likely result from altered N-linked carbohydrate expression on the beta(1) integrin subunit...
  4. pmc Molecular basis of N-acetylglucosaminyltransferase I deficiency in Arabidopsis thaliana plants lacking complex N-glycans
    Richard Strasser
    Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Institut für Angewandte Genetik und Zellbiologie, Universitat fur Bodenkultur Wien, Muthgasse 18, A 1190 Wien, Austria
    Biochem J 387:385-91. 2005
    ..These results indicate that the complete deficiency of GnTI activity in cgl plants is mainly due to the additional N-glycan, which appears to interfere with the proper folding of the enzyme...
  5. pmc Multiple osteochondromas
    Judith V M G Bovee
    Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
    Orphanet J Rare Dis 3:3. 2008
    ..For secondary peripheral chondrosarcoma, en-bloc resection of the lesion and its pseudocapsule with tumour-free margins, preferably in a bone tumour referral centre, should be performed...
  6. pmc Genotype-phenotype correlation in hereditary multiple exostoses
    C Francannet
    Service de Pédiatrie B et de Génétique, Hotel Dieu, BP 69, Clermont Ferrand, France
    J Med Genet 38:430-4. 2001
    ..These findings provide the first genotype-phenotype correlation in HME and will, it is hoped, facilitate the clinical management of these patients...
  7. pmc Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates
    E Ioffe
    Department of Cell Biology, Albert Einstein College of Medicine, New York, NY 10461
    Proc Natl Acad Sci U S A 91:728-32. 1994
    ..Biochemical and morphological analyses of embryos from 8.5 to 13.5 days of gestation showed that Mgat-1-/-embryos are developmentally retarded, most noticeably in neural tissue, and die between 9.5 and 10.5 days of development...
  8. pmc The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate
    C McCormick
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Proc Natl Acad Sci U S A 97:668-73. 2000
    ..These findings provide a rationale to explain how inherited mutations in either of the two EXT genes can cause loss of activity, resulting in hereditary multiple exostoses...
  9. ncbi Branched N-glycans regulate the biological functions of integrins and cadherins
    Yanyang Zhao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    FEBS J 275:1939-48. 2008
    ..Collectively, N-glycans on adhesion molecules, especially those on E-cadherin and integrins, play key roles in cell-cell and cell-extracellular matrix interactions, thereby affecting cancer metastasis...
  10. ncbi Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis
    K Murata
    Department of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari, Japan
    Clin Cancer Res 6:1772-7. 2000
    ..We showed direct evidence for the relationship between GnT-V and metastasis in human colorectal cancer. Screening of GnT-V expression in colorectal cancer may provide useful information for prognosis of postoperative patients...
  11. ncbi HSPG synthesis by zebrafish Ext2 and Extl3 is required for Fgf10 signalling during limb development
    William H J Norton
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Development 132:4963-73. 2005
    ..This reveals an unexpected specificity of HSPGs in regulating distinct vertebrate Fgfs...
  12. ncbi C2GnT-M is downregulated in colorectal cancer and its re-expression causes growth inhibition of colon cancer cells
    M C Huang
    Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan
    Oncogene 25:3267-76. 2006
    ..Tumor growth in vivo was also significantly suppressed by C2GnT-M in the xenografts of nude mice. These results demonstrate that C2GnT-M is frequently downregulated in colorectal cancer and suppresses colon cancer cell growth...
  13. ncbi Localisation of members of the notch system and the differentiation of vibrissa hair follicles: receptors, ligands, and fringe modulators
    B Favier
    Biologie de la Différentiation Epithéliale, UMR CNRS 5538 LEDAC, Institut Albert Bonniot, Universite Joseph Fourier, Grenoble, France
    Dev Dyn 218:426-37. 2000
    ..During the hair vibrissa cycle, Notch1 and Manic Fringe display temporal and spatial changes of expression, suggesting that they may intervene as modulators of trichocyte activities...
  14. ncbi N-acetylglucosaminyltransferase V and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer
    Hirofumi Ishimura
    Department of Urology, Hirosaki University School of Medicine, Hirosaki, Japan
    Clin Cancer Res 12:2506-11. 2006
    ..We examined the relationship between GnT-V expression and clinicopathologic features of the patients with bladder cancer...
  15. pmc Mice deficient in Ext2 lack heparan sulfate and develop exostoses
    Dominique Stickens
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    Development 132:5055-68. 2005
    ..The finding that haploinsufficiency triggers abnormal cartilage differentiation gives insight into the complex molecular mechanisms underlying the development of exostoses...
  16. ncbi The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha
    Martin Jinek
    Structural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 11:1001-7. 2004
    ..Based on this structural similarity, we propose that OGT uses an analogous molecular mechanism to recognize its targets...
  17. pmc Etiological point mutations in the hereditary multiple exostoses gene EXT1: a functional analysis of heparan sulfate polymerase activity
    P K Cheung
    Department of Microbiology and Immunology, University of British Columbia, 6174 University Boulevard, Vancouver, British Columbia, V6T 1Z3, Canada
    Am J Hum Genet 69:55-66. 2001
    ..The corresponding missense mutations may therefore represent rare genetic polymorphisms in the EXT1 gene or may interfere with as yet undefined functions of EXT1 that are involved in HME pathogenesis...
  18. ncbi Severity of disease and risk of malignant change in hereditary multiple exostoses. A genotype-phenotype study
    D E Porter
    Edinburgh University Department of Orthopaedic Surgery, New Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SU, UK
    J Bone Joint Surg Br 86:1041-6. 2004
    ..The sarcoma risk in EXT1 carriers is similar to the risk of breast cancer in an older population subjected to breast-screening, suggesting that a role for regular screening in patients with hereditary multiple exostoses is justifiable...
  19. ncbi Elevated expression of UDP-N-acetylglucosamine: alphamannoside beta1,6 N-acetylglucosaminyltransferase is an early event in hepatocarcinogenesis
    Y Ito
    Department of Surgery, Osaka Seamen's Insurance Hospital, Osaka, Japan
    Int J Cancer 91:631-7. 2001
    ..0373). These findings strongly suggest that GnT-V expression is concerned mainly with an early phase of hepatocarcinogenesis...
  20. pmc The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis
    D Mengin-Lecreulx
    Laboratoire de Biochimie Moleculaire et Cellulaire, URA 1131, Centre National de la Recherche Scientifique, Universite Paris Sud, Orsay, France
    J Bacteriol 173:4625-36. 1991
    ..In cell fractionation experiments, the transferase is essentially associated with membranes when it is recovered...
  21. ncbi Mutation screening of EXT1 and EXT2 by direct sequence analysis and MLPA in patients with multiple osteochondromas: splice site mutations and exonic deletions account for more than half of the mutations
    Geraldine R Vink
    Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands
    Eur J Hum Genet 13:470-4. 2005
    ..In patients suspected to be affected by MO, we recommend a quantitative analysis such as MLPA, followed by direct sequence analysis for the screening of the EXT1 and EXT2 genes...
  22. ncbi Evaluation of the anatomic burden of patients with hereditary multiple exostoses
    Christine M Alvarez
    University of British Columbia, Faculty of Medicine, Division of Pediatric Orthopaedics, Department of Orthopaedics, British Columbia Children s Hospital, Vancouver, BC, Canada
    Clin Orthop Relat Res 462:73-9. 2007
    ..EXT1 patients were shorter. All limb segments tended to be shorter for EXT1 subjects. EXT1 subjects showed more anatomic burden with respect to lesion quality and height...
  23. ncbi Biological consequences of overexpressing or eliminating N-acetylglucosaminyltransferase-TIII in the mouse
    Pamela Stanley
    Department of Cell Biology, Albert Einstein College Medicine, Yeshiva University, Bronx, New York, NY 10461, USA
    Biochim Biophys Acta 1573:363-8. 2002
    ..Further biological functions of GlcNAc-TIII are expected to be uncovered as mice with a null mutation in the Mgat3 gene are challenged...
  24. ncbi The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate
    C McCormick
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Nat Genet 19:158-61. 1998
    ..Two EXT1 variants containing aetiologic missense mutations failed to alter cell-surface glycosaminoglycans, despite retaining their ER-localization...
  25. pmc Isolation of a mutant Arabidopsis plant that lacks N-acetyl glucosaminyl transferase I and is unable to synthesize Golgi-modified complex N-linked glycans
    A von Schaewen
    Department of Biology, University of California, San Diego, La Jolla 92093 0116
    Plant Physiol 102:1109-18. 1993
    ..thaliana with a transgenic strain that expresses the glycoprotein phytohemagglutinin, we obtained a unique strain that synthesizes phytohemagglutinin with two high-mannose glycans, instead of one high-mannose and one complex glycan...
  26. ncbi Refining genotype phenotype correlations in muscular dystrophies with defective glycosylation of dystroglycan
    Caroline Godfrey
    Dubowitz Neuromuscular Unit, Hammersmith Hospital, Imperial College, London, UK
    Brain 130:2725-35. 2007
    ..Mutations in these five glycosyltransferase genes were detected in 34% of patients indicating that, after the exclusion of FKRP, the majority of patients with a dystroglycanopathy harbour mutations in novel genes...
  27. ncbi Roles of the tetratricopeptide repeat domain in O-GlcNAc transferase targeting and protein substrate specificity
    Sai Prasad N Iyer
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 278:24608-16. 2003
    ..These studies support the model that the catalytic subunit of OGT achieves both high specificity and a remarkable diversity of substrates by complexing with a variety of targeting proteins via its TPR protein-protein interaction domains...
  28. pmc Galectin binding to Mgat5-modified N-glycans regulates fibronectin matrix remodeling in tumor cells
    Annick Lagana
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
    Mol Cell Biol 26:3181-93. 2006
    ....
  29. ncbi Notch ligands are substrates for protein O-fucosyltransferase-1 and Fringe
    Vladislav M Panin
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:29945-52. 2002
    ..A revised, broad consensus site, C(2)X(3-5)S/TC(3) (where X(3-5) are any 3-5 amino acid residues), is proposed...
  30. ncbi The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus
    Michelle Aaron
    Department of Molecular, Cellular, and Developmental Biology, and Microbiology Program, Yale University, New Haven, CT 06520, USA
    Mol Microbiol 64:938-52. 2007
    ..Evidence suggests that bacteria use both a FtsZ-independent and a FtsZ-dependent mode of peptidoglycan synthesis to elongate, the importance of each mode depending on the timing of FtsZ assembly during elongation...
  31. ncbi Clinicopathological significance of core 2 beta1,6-N-acetylglucosaminyltransferase messenger RNA expressed in the pulmonary adenocarcinoma determined by in situ hybridization
    E Machida
    Second Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan
    Cancer Res 61:2226-31. 2001
    ..These results indicate that C2GnT mRNA detected by in situ hybridization reflects the malignant potentials of pulmonary adenocarcinoma, because lymph node metastasis is the most affecting factor to the patients' prognosis...
  32. ncbi LARGE can functionally bypass alpha-dystroglycan glycosylation defects in distinct congenital muscular dystrophies
    Rita Barresi
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Nat Med 10:696-703. 2004
    ..Our findings indicate that modulation of LARGE expression or activity is a viable therapeutic strategy for glycosyltransferase-deficient congenital muscular dystrophies...
  33. ncbi fringe and Notch specify polar cell fate during Drosophila oogenesis
    M Grammont
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA
    Development 128:2243-53. 2001
    ..Our results indicate that fringe plays a key role in positioning Notch activation during early oogenesis, and establish a function for the polar cells in separating germline cysts into individual follicles...
  34. ncbi Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 116:647-54. 2003
    ..The differential localization of mitochondrial and nucleocytoplasmic isoforms of OGT suggests that they perform unique intracellular functions...
  35. ncbi Inhibition of a specific N-glycosylation activity results in attenuation of breast carcinoma cell invasiveness-related phenotypes: inhibition of epidermal growth factor-induced dephosphorylation of focal adhesion kinase
    Hua Bei Guo
    Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
    J Biol Chem 282:22150-62. 2007
    ..These effects suppressed EGF-mediated downstream signaling and invasiveness-related phenotypes and suggest GnT-V as a potential therapeutic target...
  36. ncbi Axon sorting in the optic tract requires HSPG synthesis by ext2 (dackel) and extl3 (boxer)
    Jeong Soo Lee
    Department of Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, Utah 84103, USA
    Neuron 44:947-60. 2004
    ..This genetic evidence that heparan sulfate proteoglycan function is required for optic tract sorting provides clues to begin understanding the underlying molecular mechanisms...
  37. ncbi Tout-velu is a Drosophila homologue of the putative tumour suppressor EXT-1 and is needed for Hh diffusion
    Y Bellaiche
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 394:85-8. 1998
    ..These results show the existence of a new conserved mechanism required for diffusion of Hedgehog...
  38. ncbi Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc
    L Wells
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 USA
    Science 291:2376-8. 2001
    ..Here we systematically examine the current data implicating O-GlcNAc as a regulatory modification important to signal transduction cascades...
  39. pmc The link between heparan sulfate and hereditary bone disease: finding a function for the EXT family of putative tumor suppressor proteins
    G Duncan
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    J Clin Invest 108:511-6. 2001
  40. ncbi Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation
    M Demetriou
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 409:733-9. 2001
    ..Dysregulation of Mgat5 in humans may increase susceptibility to autoimmune diseases, such as multiple sclerosis...
  41. pmc Core 3 synthase is down-regulated in colon carcinoma and profoundly suppresses the metastatic potential of carcinoma cells
    Toshie Iwai
    Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, OSL C 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Proc Natl Acad Sci U S A 102:4572-7. 2005
    ..v. injection of the transfectants into nude mice was significantly suppressed. These findings indicated that the core structures of O-glycans are profoundly involved in the metastatic capacity of cancer cells...
  42. ncbi Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats
    L K Kreppel
    Department of Biochemistry and Molecular Genetics Schools of Medicine Dentistry, University of Alabama at Birmingham Station, Birmingham, Alabama 35294, USA
    J Biol Chem 272:9308-15. 1997
    ..The OGT is also modified by tyrosine phosphorylation, indicating that tyrosine kinase signal transduction cascades may play a role in modulating OGT activity...
  43. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
    ..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
  44. ncbi Deficiency of alpha-dystroglycan in muscle-eye-brain disease
    Hiroki Kano
    Division of Functional Genomics, Osaka University Graduate School of Medicine, 2 2 B9, Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 291:1283-6. 2002
    ..This finding suggests that alpha-dystroglycan is a potential target of POMGnT1 and that altered glycosylation of alpha-dystroglycan may play a critical role in the pathomechanism of MEB and some forms of muscular dystrophy...
  45. ncbi Recombinant O-GlcNAc transferase isoforms: identification of O-GlcNAcase, yes tyrosine kinase, and tau as isoform-specific substrates
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, MD 20897 0851, USA
    Glycobiology 16:415-21. 2006
    ..The identification of a tyrosine kinase and O-GlcNAcase as OGT targets suggests the potential for OGT participation in numerous signal transduction cascades...
  46. ncbi The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin
    Bruce Walcheck
    Department of Veterinary Pathobiology, University Minnesota Academic Health Center, University of Minnesota, St Paul 55108, USA
    Blood 99:4063-9. 2002
    ..These findings support evidence that differential C2GnT activity results in T-cell subsets that express ligands for E-selectin, P-selectin, or both...
  47. ncbi Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching
    Shinji Ihara
    Department of Biochemistry, Osaka University Medical School graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:16960-7. 2002
    ..These results indicate that this process is a key element in malignant transformation, as the direct result of oligosaccharide modification...
  48. ncbi Decreased EXT expression and intracellular accumulation of heparan sulphate proteoglycan in osteochondromas and peripheral chondrosarcomas
    L Hameetman
    Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands
    J Pathol 211:399-409. 2007
    ..We hypothesize that loss of EXT expression disrupts the function of the EXT1/2 complex in HSPG biosynthesis, resulting in the intracellular accumulation of HSPG core proteins that we found in these tumours...
  49. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
    ..These findings expand our understanding of the mechanisms that underlie congenital muscular dystrophy...
  50. ncbi Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation
    Carlos Martinez-Fleites
    Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington, York YO10 5YW, UK
    Nat Struct Mol Biol 15:764-5. 2008
    ..UDP-GlcNAc:polypeptidyl transferase (OGT) catalyzes O-GlcNAc addition. The structure of an intact OGT homolog and kinetic analysis of human OGT variants reveal a contiguous superhelical groove that directs substrates to the active site...
  51. pmc Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells
    Akira Seko
    Innovative Research Initiatives, Tokyo Institute of Technology, Yokohama 226 8503, Japan
    J Biol Chem 283:33094-100. 2008
    ..These results raise a possibility that up-regulation of beta3Gn-T8 in differentiated HL-60 cells increases poly-N-acetyllactosamine chains by activating intrinsic beta3Gn-T2...
  52. ncbi An optimized DHPLC protocol for molecular testing of the EXT1 and EXT2 genes in hereditary multiple osteochondromas
    W Wuyts
    Department of Medical Genetics, University of Antwerp, Belgium
    Clin Genet 68:542-7. 2005
    ..The protocol described here, therefore, provides a sensitive and cost-sparing alternative for direct sequencing analysis of the MO-causing genes...
  53. ncbi Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1
    A Yoshida
    Central Laboratories for Key Technology, Kirin Brewery Co, Ltd, Kanazawa Ku, 236 0004, Yokohama, Japan
    Dev Cell 1:717-24. 2001
    ..These findings suggest that interference in O-mannosyl glycosylation is a new pathomechanism for muscular dystrophy as well as neuronal migration disorder...
  54. ncbi Determination of the mutation spectrum of the EXT1/EXT2 genes in British Caucasian patients with multiple osteochondromas, and exclusion of six candidate genes in EXT negative cases
    Lorne Lonie
    The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    Hum Mutat 27:1160. 2006
    ..This technique was found to be sensitive with a detection rate of 100% regarding heterozygote detection for EXT mutation scanning. Furthermore, this technique has a very high throughput and is very cost-effective...
  55. ncbi Expression of N-acetylglucosaminyltransferase v is associated with prognosis and histology in non-small cell lung cancers
    Hirotoshi Dosaka-Akita
    Department of Medical Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Clin Cancer Res 10:1773-9. 2004
    ..The objective of this study is to examine GnT-V expression in non-small cell lung cancers (NSCLCs) and to determine its relationship to biological and clinicopathological characteristics and prognosis...
  56. ncbi A novel EXT1 splice site mutation in a kindred with hereditary multiple exostosis and osteoporosis
    Manuel C Lemos
    Academic Endocrine Unit, Nuffield Department of Clinical Medicine, University of Oxford, Oxford Center for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Headington, Oxford OX3 7LJ, United Kingdom
    J Clin Endocrinol Metab 90:5386-92. 2005
    ..Hereditary multiple exostosis (HME) is an autosomal dominant disorder characterized by the development of benign cartilage-capped tumors at the juxta-epiphyseal regions of long bones. HME is usually caused by mutations of EXT1 or EXT2...
  57. ncbi Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies
    Prabhjit K Grewal
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
    Glycobiology 15:912-23. 2005
    ..Thus, there may be functional redundancy between LARGE1 and LARGE2. Consistent with this idea, we show that alpha-DG is still fully glycosylated in kidney (a tissue that expresses a high level of LARGE2 mRNA) of Large(myd) mutant mice...
  58. ncbi Evaluation of locus heterogeneity and EXT1 mutations in 34 families with hereditary multiple exostoses
    W H Raskind
    Department of Medicine, University of Washington, Seattle 98195, USA
    Hum Mutat 11:231-9. 1998
    ..Combined mutational and heterogeneity analyses in this set of families with multiple exostoses suggest that 66% of our total sample, including 45% of isolated and 77% of familial cases, are attributable to abnormalities in EXT1...
  59. pmc Ectopic expression of N-acetylglucosaminyltransferase III in transgenic hepatocytes disrupts apolipoprotein B secretion and induces aberrant cellular morphology with lipid storage
    Y Ihara
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, 1 8 Yamadaoka, Suita, Osaka 565, Japan
    Proc Natl Acad Sci U S A 95:2526-30. 1998
    ..This observation suggests a novel mechanism for the pathogenesis of fatty liver...
  60. pmc Mutations in the EXT1 and EXT2 genes in hereditary multiple exostoses
    W Wuyts
    Department of Medical Genetics, University of Antwerp, Belgium
    Am J Hum Genet 62:346-54. 1998
    ..The development is thus mainly due to loss of function of the EXT genes, consistent with the hypothesis that the EXT genes have a tumor- suppressor function...
  61. ncbi Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta
    Mari Tomiie
    Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita City, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 330:999-1004. 2005
    ..These results suggest that GnT-V would contribute to placentation in the early phase of pregnancy, possibly regulating the process of invasion of trophoblast cells...
  62. ncbi E. Coli MurG: a paradigm for a superfamily of glycosyltransferases
    S Ha
    Chemistry Department, Princeton University, Princeton, NJ 08544, USA
    Curr Drug Targets Infect Disord 1:201-13. 2001
    ..A better understanding of MurG could lead to the development of new drugs to combat antibiotic resistant infections, and may also shed light on a broad class of glycosyltransferases...
  63. ncbi Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance
    Xiaoyong Yang
    Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 451:964-9. 2008
    ..These findings identify a molecular mechanism by which nutritional cues regulate insulin signalling through O-GlcNAc, and underscore the contribution of this modification to the aetiology of insulin resistance and type 2 diabetes...
  64. ncbi Down-regulation of hydrogen peroxide-induced PKC delta activation in N-acetylglucosaminyltransferase III-transfected HeLaS3 cells
    Yukinao Shibukawa
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 278:3197-203. 2003
    ..These findings suggest that the overexpression of GnT-III suppresses H(2)O(2)-induced activation of PKC delta-JNK1 pathway, resulting in inhibition of apoptosis...
  65. ncbi Mutation frequencies of EXT1 and EXT2 in 43 Japanese families with hereditary multiple exostoses
    H Seki
    Department of Orthopedic Surgery, Shinshu University School of Medicine, Nagano, Japan
    Am J Med Genet 99:59-62. 2001
    ..These results expand our knowledge of the ethnic difference of EXT and the structure-function relationship of the EXT genes...
  66. ncbi Molecular cloning and characterization of a novel UDP-GlcNAc:GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase (beta 3Gn-T6), an enzyme synthesizing the core 3 structure of O-glycans
    Toshie Iwai
    Laboratory of Gene Function Analysis, Institute of Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology AIST, Open Space Laboratory C 2, 1 1 1 Umezono, Japan
    J Biol Chem 277:12802-9. 2002
    ..We concluded that beta3Gn-T6 is the most logical candidate for the core 3 synthase, which plays an important role in the synthesis of mucin-type O-glycans in digestive organs...
  67. ncbi Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis
    Emily A Partridge
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada
    Science 306:120-4. 2004
    ..Thus, conditional regulation of N-glycan processing drives synchronous modification of cytokine receptors, which balances their surface retention against loss via endocytosis...
  68. ncbi Regulation of N-acetylglucosaminyltransferase V and Asn-linked oligosaccharide beta(1,6) branching by a growth factor signaling pathway and effects on cell adhesion and metastatic potential
    M Pierce
    Department of Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, University of Georgia, Athens 30605, USA
    Glycoconj J 14:623-30. 1997
    ....
  69. ncbi Overexpression of N-acetylglucosaminyltransferase III enhances the epidermal growth factor-induced phosphorylation of ERK in HeLaS3 cells by up-regulation of the internalization rate of the receptors
    Y Sato
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
    J Biol Chem 276:11956-62. 2001
    ..These results suggest that GnT-III overexpression in HeLaS3 cells resulted in an enhancement of EGF-induced ERK phosphorylation at least in part by the up-regulation of the endocytosis of EGFR...
  70. ncbi Expression of fringe is down regulated by Gurken/Epidermal Growth Factor Receptor signalling and is required for the morphogenesis of ovarian follicle cells
    D Zhao
    The University of Edinburgh, Institute of Cell and Molecular Biology, Darwin Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    J Cell Sci 113:3781-94. 2000
    ..This has been confirmed by re-examination of fng mutants and analysis of fng mutant clones in oogenesis...
  71. ncbi Fringe is a glycosyltransferase that modifies Notch
    D J Moloney
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, SUNY Stony Brook, New York 11794 5215, USA
    Nature 406:369-75. 2000
    ....
  72. ncbi Regulation of a cytosolic and nuclear O-GlcNAc transferase. Role of the tetratricopeptide repeats
    L K Kreppel
    Department of Biological Chemistry, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 274:32015-22. 1999
    ....
  73. pmc Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability
    Niall O'Donnell
    Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093 0625, USA
    Mol Cell Biol 24:1680-90. 2004
    ..We find that O-GlcNAc modulates protein phosphorylation and expression among essential and conserved cell signaling pathways...
  74. ncbi The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1
    M Dalziel
    Imperial Cancer Research Fund, Breast Cancer Biology Group, 3rd Floor, Thomas Guy House, Guy's Hospital, London SE1 9RT, United Kingdom
    J Biol Chem 276:11007-15. 2001
    ..Thus, even when C2GnT1 is expressed, the O-glycans added to MUC1 become core 1-dominated structures, provided expression of ST3Gal-I is increased as it is in breast cancer...
  75. pmc The bisecting GlcNAc on N-glycans inhibits growth factor signaling and retards mammary tumor progression
    Yinghui Song
    Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    Cancer Res 70:3361-71. 2010
    ..Thus, the addition of the bisecting GlcNAc to complex N-glycans of mammary tumor cell glycoprotein receptors is a cell autonomous mechanism serving to retard tumor progression by reducing growth factor signaling...
  76. pmc Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development
    M Metzler
    Biomedical Research Centre, University of British Columbia, Vancouver, Canada
    EMBO J 13:2056-65. 1994
    ..Complex N-linked oligosaccharides are important for morphogenic processes as neural tube formation, vascularization and the determination of left-right body plan asymmetry were impaired in the absence of a functional Mgat-1 gene...
  77. ncbi Mutant glycosyltransferase and altered glycosylation of alpha-dystroglycan in the myodystrophy mouse
    P K Grewal
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
    Nat Genet 28:151-4. 2001
    ..We speculate that abnormal post-translational modification of alpha-dystroglycan may contribute to the myd phenotype...
  78. pmc Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity
    Ronald J Copeland
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Am J Physiol Endocrinol Metab 295:E17-28. 2008
    ....
  79. ncbi Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression
    Xiaoyong Yang
    Department of Medicine, Division of Endocrinology and Metabolism, Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Cell 110:69-80. 2002
    ....
  80. ncbi Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 123:1307-21. 2005
    ..We infer that beta cell glucose-transporter glycosylation mediates a link between diet and insulin production that typically suppresses the pathogenesis of type 2 diabetes...
  81. ncbi Severe impairment of leukocyte rolling in venules of core 2 glucosaminyltransferase-deficient mice
    M Sperandio
    Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Blood 97:3812-9. 2001
    ..In conclusion, P-selectin ligands including PSGL-1 are largely C2GlcNAcT-I dependent. In addition, E-selectin-mediated rolling in vivo is partially dependent on the targeted C2GlcNAcT-I. (Blood. 2001;97:3812-3819)..
  82. ncbi The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate
    T Lind
    Department of Medical Biochemistry and Microbiology, Uppsala University, The Biomedical Center, Box 575, S 751 23 Uppsala, Sweden
    J Biol Chem 273:26265-8. 1998
    ..Thus at least two members of the EXT family of tumor suppressors encode glycosyltransferases involved in the chain elongation step of HS biosynthesis...
  83. ncbi Ext1-dependent heparan sulfate regulates the range of Ihh signaling during endochondral ossification
    Lydia Koziel
    Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Dev Cell 6:801-13. 2004
    ..Finally, we propose that the development of exostoses in the human Hereditary Multiple Exostoses syndrome can be attributed to activation of Ihh signaling...
  84. ncbi Ocular abnormalities in Large(myd) and Large(vls) mice, spontaneous models for muscle, eye, and brain diseases
    Yongsuk Lee
    The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
    Mol Cell Neurosci 30:160-72. 2005
    ..Finally, hypoglycosylation of alpha-dystroglycan previously implicated in muscle and brain defects is also observed in the retina and may contribute to the ocular abnormalities...
  85. ncbi Glyc-O-genetics of Walker-Warburg syndrome
    J van Reeuwijk
    Department of Human Genetics, Radboud University Nijmegen Medical Center, The Netherlands
    Clin Genet 67:281-9. 2005
    ..Perhaps, the missing genes underlying the remainder of the unexplained WWS patients have also to be sought in the pathways involved in O-linked protein glycosylation...
  86. ncbi A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity
    Takashi Saito
    Department of Biochemistry, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:17002-8. 2002
    ..These findings provide some novel information on the relationship between GnT-V and tumor metastasis. The inhibition of GnT-V secretion or its expression represents a novel potential strategy for the inhibition of tumor angiogenesis...
  87. ncbi Expression of the high-affinity selectin glycan ligand C2-O-sLeX by colon carcinoma cells
    Catherine A St Hill
    Department of Veterinary and Biomedical Sciences, University of Minnesota Twin Cities campuses, Minneapolis St Paul, MN 55108, USA
    Cancer Lett 217:105-13. 2005
    ..Hence, we demonstrate for the first time the detection of C2-O-sLeX on colon carcinoma cells, which, as with leukocytes, may be directly relevant to the expression of high affinity glycan ligands for the selectins...
  88. ncbi Mild POMGnT1 mutations underlie a novel limb-girdle muscular dystrophy variant
    Emma M Clement
    Dubowitz Neuromuscular Unit, Department of Paediatrics, Hammersmith Hospital, Imperial College London, Du Cane Road, London W12 ONN, England
    Arch Neurol 65:137-41. 2008
    ..Mutations in protein-O-mannose-beta1,2-N-acetylglucosaminyltransferase 1 (POMGnT1) have been found in muscle-eye-brain disease, a congenital muscular dystrophy with structural eye and brain defects and severe mental retardation...
  89. ncbi Mouse large can modify complex N- and mucin O-glycans on alpha-dystroglycan to induce laminin binding
    Santosh K Patnaik
    Department of Cell Biology, Albert Einstein College of Medicine, New York, New York 10461, USA
    J Biol Chem 280:20851-9. 2005
    ..In addition, the data suggest that human LARGE may restore functional alpha-DG to muscle cells from patients with defective synthesis of O-mannose glycans via the modification of N-glycans and/or mucin O-glycans on alpha-DG...
  90. ncbi Breaches of the pial basement membrane and disappearance of the glia limitans during development underlie the cortical lamination defect in the mouse model of muscle-eye-brain disease
    Huaiyu Hu
    Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, New York 13210, USA
    J Comp Neurol 501:168-83. 2007
    ....
  91. ncbi Does O-GlcNAc play a role in neurodegenerative diseases?
    Tony Lefebvre
    UMR 8576, Centre National de la Recherche Scientifique, Laboratoire de Chimie Biologique, Batiment C9, 59655 Villeneuve d Ascq, France
    Expert Rev Proteomics 2:265-75. 2005
    ..The data presented in this review strongly suggest that O-GlcNAc could play an active role in neurodegenerative diseases...
  92. ncbi Selective decrease of membrane-associated PKC-alpha and PKC-epsilon in response to elevated intracellular O-GlcNAc levels in transformed human glial cells
    Jason A Matthews
    Department of Chemistry, University of South Florida, 4202 East Fowler Ave, SCA 400, Tampa, FL, 33620, USA
    Biochim Biophys Acta 1743:305-15. 2005
    ..In addition, none of the three compounds affected membrane levels of PKC-iota. Altogether, these results demonstrate a novel link between increased levels of the O-GlcNAc modification and the regulation of specific PKC isoforms...
  93. pmc Cloning and expression of a novel UDP-GlcNAc:alpha-D-mannoside beta1,2-N-acetylglucosaminyltransferase homologous to UDP-GlcNAc:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I
    Wenli Zhang
    Program in Structural Biology and Biochemistry, The Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Biochem J 361:153-62. 2002
    ..2 may be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins...
  94. ncbi An Arabidopsis thaliana cDNA complements the N-acetylglucosaminyltransferase I deficiency of CHO Lec1 cells
    H Bakker
    Department of Molecular Biology, Centre for Plant Breeding and Reproduction Research CPRO DLO, Wageningen, 6700AA, The Netherlands
    Biochem Biophys Res Commun 261:829-32. 1999
    ..thaliana mutant (cgl-1). Here it is shown that the reverse is also true, the plant glycosyltransferase is able to complement a mammalian mutant (Lec1) deficient in GlcNAcT-I...
  95. ncbi Isolation and characterization of the murine homolog of the human EXT2 multiple exostoses gene
    D Stickens
    McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas 75235 8591, USA
    Biochem Mol Med 61:16-21. 1997
    ..The identification of the mouse EXT2 gene will allow functional analysis through insertional inactivation and reverse genetics in mice in order to better understand the formation of exostoses during bone formation...
  96. ncbi Mutational analysis of the catalytic domain of O-linked N-acetylglucosaminyl transferase
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:35537-44. 2005
    ..Identification of viable OGT mutants may facilitate examination of its role in nutrient sensing and signal transduction cascades...
  97. ncbi Disruption of perlecan binding and matrix assembly by post-translational or genetic disruption of dystroglycan function
    Motoi Kanagawa
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Roy J and Lucille A Carver College of Medicine, The University of Iowa, 400 Eckstein Medical Building, Iowa City, IA 52242, USA
    FEBS Lett 579:4792-6. 2005
    ..These data suggest functional disruption of the trimolecular complex in glycosylation-deficient muscular dystrophy...
  98. pmc A Caenorhabditis elegans model of insulin resistance: altered macronutrient storage and dauer formation in an OGT-1 knockout
    John A Hanover
    Laboratories of Cell Biochemistry and Biology and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:11266-71. 2005
    ..Our findings demonstrate that OGT modulates macronutrient storage and dauer formation in C. elegans, providing a unique genetic model for examining the role of O-GlcNAc in cellular signaling and insulin resistance...
  99. ncbi Progression of hepatic neoplasms is severely retarded in mice lacking the bisecting N-acetylglucosamine on N-glycans: evidence for a glycoprotein factor that facilitates hepatic tumor progression
    M Bhaumik
    Department of Cell Biology, Albert Einstein College Medicine, New York, New York 10461, USA
    Cancer Res 58:2881-7. 1998
    ..In the absence of the bisecting GlcNAc in Mgat3-/- mice, the factor is reduced in activity, and tumor progression is severely retarded...
  100. ncbi Differentiation and developmental origin of cerebellar granule neuron ectopia in protein O-mannose UDP-N-acetylglucosaminyl transferase 1 knockout mice
    X Li
    Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, NY 13210, USA
    Neuroscience 152:391-406. 2008
    ..Moreover, migration into the IGL is not required for granule cell acquisition of certain differentiated characteristics...
  101. ncbi Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1
    Gipsy Majumdar
    Research Services, VA Medical Center, Memphis, Tennessee, USA
    Diabetes 53:3184-92. 2004
    ..We conclude that insulin stimulates CaM gene transcription via a dynamic interplay between O-glycosylation and phosphorylation of Sp1 that modulates stability, mobility, subcellular compartmentalization, and activity...

Research Grants44

  1. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..Here we further explore the mechanisms by which protein glycosylation regulates immune function and autoimmunity. ..
  2. O-Glycosylation of Epidermal Growth Factor-like Motifs
    Robert S Haltiwanger; Fiscal Year: 2013
    ....
  3. GLYCOSYLTRANSFERASE GENE EXPRESSION IN TRANSFORMED CELLS
    Nevis Fregien; Fiscal Year: 1992
    ..These probe will also be used to determine the molecular basis of the lesions in the mutant cells lines...
  4. GLYCOPROTEINS IN DIFFERENTIATION AND ONCOGENESIS
    Minoru Fukuda; Fiscal Year: 2009
    ..pylori colonization, thus early onset of gastric cancer and if inactivation of another Core2GlcNAcTs leads to attenuated lymphocyte recruitment, thus impairing inflammation and delaying the onset of gastric cancer. ..
  5. Polylactosamines in Development and Cancer
    Minoru Fukuda; Fiscal Year: 2007
    ..The studies will also determine if iGnT plays critical role in development and suppressing tumor formation through the gene knockout of iGnT and analysis of development and tumor formation in those mutant mice. ..
  6. NEURONAL MIGRATION IN THE DEVELOPING BRAIN
    Huaiyu Hu; Fiscal Year: 2003
    ....
  7. Molecular Studies of Brain Malformations
    Huaiyu Hu; Fiscal Year: 2007
    ....
  8. Genetic Studies of Clubfoot (ITEV)
    Jacqueline T Hecht; Fiscal Year: 2010
    ..Identification of high-risk genotypes can lead to the development of prevention programs in selected populations and may suggest gene-based prevention strategies. ..
  9. Strategy for Therapy of the Pseudoachondroplasia (PSACH) Phenotype: Understanding
    Jacqueline Hecht; Fiscal Year: 2006
    ..abstract_text> ..
  10. O-GlcNAc:Developing New Tool for Assessment of Glycemia
    Gerald Hart; Fiscal Year: 2008
    ..The project also represents a new inter-disciplinary, inter-departmental basic science-clinical research collaboration between Biological Chemistry and The Department of Medicine. ..
  11. Galgt2, dystroglycan, and muscle extracellular matrix
    Paul Martin; Fiscal Year: 2008
    ..These experiments will decipher mechanisms responsible for the functional roles of Galgt2 in muscle and neuromuscular development, as well as for its therapeutic role in muscular dystrophy. ..
  12. Identification of novel bioactive glycans on dystroglycan
    Paul Martin; Fiscal Year: 2007
    ..This proposal, therefore, will not only define a novel function for a therapeutically important enzyme, but likely identify a glycan structure not yet described in mammals. [unreadable] [unreadable]..
  13. Histone Acetylation and Insulin Gene Expression
    Sabire Ozcan; Fiscal Year: 2010
    ..In addition, the data obtained will contribute to our understanding of Pdx-1, MafA and NeuroD1 function in the regulation of pancreas development and glucose-stimulated insulin gene transcription. ..
  14. SELECTING ENV TRIMERS IN HIV-VLP VACCINE PRESENTATIONS
    James Binley; Fiscal Year: 2007
    ..In Specific Aim 3, we will test the immunogenicity of i) pseudovirions bearing only functional trimers and ii) receptor-engaged pseudovirions attached to autologous macaque lymphocytes. ..
  15. Sexually Transmitted Infections & Antimicrobial Peptides
    William Shafer; Fiscal Year: 2009
    ..abstract_text> ..
  16. GONOCOCCI: GENETICS OF RESISTANCE TO PMN PROTEINS
    William Shafer; Fiscal Year: 2009
    ..abstract_text> ..
  17. Structure-function studies of endotoxin receptor CD14
    Nitin Jain; Fiscal Year: 2009
    ..Completion of these studies will provide the structural basis for the deleterious effects of LPS, PGN and rational design of potentially novel LPS, PGN antagonists for treatment of sepsis. ..
  18. DEVELOPMENT OF STAPHYLOCOCCALCIDAL HUMAN PEPTIDES
    William Shafer; Fiscal Year: 2008
    ..This information should help in the development of new effective peptide-based drugs that could be used in the future to combat staphylococcal diseases caused by antibiotic-resistant strains. ..
  19. Regulation of inflammation: sheddases and CD62L
    Bruce Walcheck; Fiscal Year: 2009
    ..This knowledge will be highly useful in the development of more specific therapies tailored to inflammatory processes and immunity. ..
  20. 2009 Multi-drug Efflux Systems Gordon Research Conference
    William Shafer; Fiscal Year: 2008
    ..The GRC will focus on these specific key areas related to multi-drug efflux pumps in resistance. [unreadable] [unreadable] [unreadable]..
  21. Wall Teichoic Acid Biosynthesis: A New Target for Therapeutic Intervention?
    SUZANNE L WALKER; Fiscal Year: 2010
    ..aureus and about some of the more interesting WTA enzymes. ..
  22. Glycosyltransferase Therapy for Myopathies
    PAUL TAYLOR MARTIN; Fiscal Year: 2010
    ..Another long-term goal is to develop a novel connection between glycosylation and myostatin signaling that may more broadly impact therapies for muscle aging and disease. ..
  23. Role of Na, K-ATPase beta isoforms in pump sorting and epithelial integrity
    Olga Vagin; Fiscal Year: 2010
    ....
  24. Cellular receptors for pathogenic New World arenaviruses
    Stefan Kunz; Fiscal Year: 2007
    ..In a complementary approach, biochemical techniques, combined with mass spectrometry, will therefore be used for the identification of candidate receptors for this important human pathogen. [unreadable] [unreadable] [unreadable]..
  25. GLYCOCONJUGATES IN DEVELOPMENT AND IMMUNITY
    Gerald Hart; Fiscal Year: 2007
    ..These studies are fundamentally important to the regulation of transcription and signal transduction processes important to virtually all aspects of development and disease. ..
  26. Regulation of Insulin Gene Expression in Liver Cells
    Sabire Ozcan; Fiscal Year: 2004
    ..The development of such non-beta cell lines will be important for the treatment of type I and as well as type II diabetes. ..
  27. UNDERSTANDING THE PATHOGENESIS OF STAPHYLOCOCCUS AUREUS
    Vance Fowler; Fiscal Year: 2003
    ..Accomplishing the aims of this proposal will contribute to improved clinical management of patients with S. aureus bacteremia, and will add to the understanding of S. aureus virulence factors. ..
  28. RSV AND ASTHMA
    David Broide; Fiscal Year: 2003
    ..Overall these studies will contribute to our understanding of the mechanism of eosinophilic inflammation during RSV infection and suggest candidate vaccine strategies to inhibit RSV infection and airway inflammation. ..
  29. Developing a mouse model to study Notch furin processing
    GERALDINE WEINMASTER; Fiscal Year: 2002
    ....
  30. CARBOHYDRATE AND NEUROMUSCULAR SYNAPTOGENESIS
    Paul Martin; Fiscal Year: 2002
    ..The identification of a role for Ga1NAc in the targeting of synaptic proteins or in synaptic signaling at the neuromuscular junction would provide new therapeutic avenues for the treatment of neuromuscular diseases. ..
  31. NOTCH SIGNALING IN MAMMALIAN CELLS
    GERALDINE WEINMASTER; Fiscal Year: 2002
    ..Together the experiments proposed in this grant will provide new information on the structure/function requirements of Notch-ligand interactions and thereby extend our present understanding of mammalian Notch signaling. ..
  32. ENDOTHELIUM--SIGNALING FROM MAST CELLS TO EOSINOPHILS
    David Broide; Fiscal Year: 2002
    ....
  33. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  34. Analysis of fringe-dependent cell-signaling
    Kenneth Irvine; Fiscal Year: 2004
    ....
  35. Distinction of skin homing T cells that bind P-selectin
    Bruce Walcheck; Fiscal Year: 2004
    ..abstract_text> ..
  36. Synthesis as a Tool to Understand Ramoplanin and Nisin
    Suzanne Walker; Fiscal Year: 2006
    ..E) To explore the feasibility of designing hybrid antibiotics targeted to bacterial cells by attaching the Lipid II binding domain of nisin to the pore forming antibiotic magainin. ..
  37. Identification of O-Glc NAc Transferase Inhibitors(RMI)
    Suzanne Walker; Fiscal Year: 2005
    ..In addition to elucidating the role of O-GlcNAc in cellular processes, inhibitors of OGT might be useful in studying and perhaps treating type 2 diabetes. ..
  38. Structural and Mechanistic Studies on MurG
    Suzanne Walker; Fiscal Year: 2006
    ..The former goal could lead to the development of new drugs; the latter should facilitate efforts to understand the cellular roles of glycosyltransferases and their products. ..
  39. Understanding cell biology of Delta-Notch interactions
    GERALDINE WEINMASTER; Fiscal Year: 2006
    ....
  40. Biomarkers in sputum in COPD
    David Broide; Fiscal Year: 2005
    ..abstract_text> ..
  41. Eosinophil Trafficking
    David Broide; Fiscal Year: 2005
    ..Overall, these studies should improve our understanding of the molecular mechanism of eosinophil recruitment in vivo and its effect on airway structure and function. ..
  42. Molecular Mechanisms of Segmentation
    Cordelia Rauskolb; Fiscal Year: 2005
    ..abstract_text> ..
  43. Role of O-GlcNAc transferase in insulin gene expression
    Sabire Ozcan; Fiscal Year: 2005
    ..In addition, the obtained data will be instrumental in the design of novel strategies for the treatment and prevention of type I and type II diabetes and the secondary complications associated with diabetes. ..
  44. INVESTIGATING A ROLE FOR NOTCH SIGNALING IN ANGIOGENESIS
    GERALDINE WEINMASTER; Fiscal Year: 2001
    ..abstract_text> ..