peptidyl transferases

Summary

Summary: Acyltransferases that use AMINO ACYL TRNA as the amino acid donor in formation of a peptide bond. There are ribosomal and non-ribosomal peptidyltransferases.

Top Publications

  1. ncbi Structure of the 70S ribosome complexed with mRNA and tRNA
    Maria Selmer
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 313:1935-42. 2006
  2. pmc Structural insight into nascent polypeptide chain-mediated translational stalling
    Birgit Seidelt
    Gene Center and Center for Integrated Protein Science Munich CIPSM, Department for Chemistry and Biochemistry, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Science 326:1412-5. 2009
  3. pmc A molecular clamp ensures allosteric coordination of peptidyltransfer and ligand binding to the ribosomal A-site
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Nucleic Acids Res 38:7800-13. 2010
  4. ncbi beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
  5. pmc The peptidoglycan of Mycobacterium abscessus is predominantly cross-linked by L,D-transpeptidases
    Marie Lavollay
    LRMA, Equipe 12, Centre de Recherches des Cordeliers, 15 rue de l Ecole de Medecine, 75270 Paris Cedex 06, France
    J Bacteriol 193:778-82. 2011
  6. ncbi Structures of the bacterial ribosome at 3.5 A resolution
    Barbara S Schuwirth
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 310:827-34. 2005
  7. ncbi Staphylococcus aureus Sortase A transpeptidase. Calcium promotes sorting signal binding by altering the mobility and structure of an active site loop
    Mandar T Naik
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1570, USA
    J Biol Chem 281:1817-26. 2006
  8. ncbi Structural basis for translational stalling by human cytomegalovirus and fungal arginine attenuator peptide
    Shashi Bhushan
    Gene Center and Department of Biochemistry and Center for integrated Protein Science Munich, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 40:138-46. 2010
  9. ncbi Heavy metal detoxification in higher plants--a review
    M H Zenk
    Lehrstuhl fur Pharmazeutische Biologie, Ludwig Maximilians Universitat Munchen, Germany
    Gene 179:21-30. 1996
  10. ncbi Recruitment of penicillin-binding protein PBP2 to the division site of Staphylococcus aureus is dependent on its transpeptidation substrates
    Mariana G Pinho
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 55:799-807. 2005

Research Grants

  1. CRYSTALLOGRAPHIC STUDIES ON RIBOSOMAL PARTICLES
    ADA E YONATH; Fiscal Year: 2010
  2. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
  3. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
  4. NMR Console Upgrade and Cryoprobe
    Peter Moore; Fiscal Year: 2002
  5. STRUCTURE AND PHYSICAL PROPERTIES OF RNA
    Peter Moore; Fiscal Year: 2007
  6. RIBOSOME STRUCTURE/FUNCTION
    Peter Moore; Fiscal Year: 2001
  7. Upgrade of X-Ray Diffractometers
    Peter Moore; Fiscal Year: 2005
  8. REVERSE TRANSCRIPTASE AND OTHER HIV MOLECULAR STRUCTURES
    Thomas Steitz; Fiscal Year: 2002
  9. Structural studies on ribosomes and antibiotics
    VENKATRAMAN RAMAKRISHNAN; Fiscal Year: 2006
  10. Characterization of the SARSCoV frameshift signal
    Jonathan D Dinman; Fiscal Year: 2010

Detail Information

Publications215 found, 100 shown here

  1. ncbi Structure of the 70S ribosome complexed with mRNA and tRNA
    Maria Selmer
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 313:1935-42. 2006
    ..The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation...
  2. pmc Structural insight into nascent polypeptide chain-mediated translational stalling
    Birgit Seidelt
    Gene Center and Center for Integrated Protein Science Munich CIPSM, Department for Chemistry and Biochemistry, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Science 326:1412-5. 2009
    ..We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel...
  3. pmc A molecular clamp ensures allosteric coordination of peptidyltransfer and ligand binding to the ribosomal A-site
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Nucleic Acids Res 38:7800-13. 2010
    ..The observation that these mutations affected translational fidelity, virus propagation and cell growth demonstrates how small structural changes at the atomic scale can propagate outward to broadly impact the biology of cell...
  4. ncbi beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
    ....
  5. pmc The peptidoglycan of Mycobacterium abscessus is predominantly cross-linked by L,D-transpeptidases
    Marie Lavollay
    LRMA, Equipe 12, Centre de Recherches des Cordeliers, 15 rue de l Ecole de Medecine, 75270 Paris Cedex 06, France
    J Bacteriol 193:778-82. 2011
    ....
  6. ncbi Structures of the bacterial ribosome at 3.5 A resolution
    Barbara S Schuwirth
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 310:827-34. 2005
    ....
  7. ncbi Staphylococcus aureus Sortase A transpeptidase. Calcium promotes sorting signal binding by altering the mobility and structure of an active site loop
    Mandar T Naik
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1570, USA
    J Biol Chem 281:1817-26. 2006
    ....
  8. ncbi Structural basis for translational stalling by human cytomegalovirus and fungal arginine attenuator peptide
    Shashi Bhushan
    Gene Center and Department of Biochemistry and Center for integrated Protein Science Munich, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Mol Cell 40:138-46. 2010
    ..Our findings provide direct structural insight into two distinct eukaryotic stalling processes...
  9. ncbi Heavy metal detoxification in higher plants--a review
    M H Zenk
    Lehrstuhl fur Pharmazeutische Biologie, Ludwig Maximilians Universitat Munchen, Germany
    Gene 179:21-30. 1996
    ..PC synthase will be an interesting target for biotechnological modification of heavy metal tolerance in higher plants...
  10. ncbi Recruitment of penicillin-binding protein PBP2 to the division site of Staphylococcus aureus is dependent on its transpeptidation substrates
    Mariana G Pinho
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 55:799-807. 2005
    ..In methicillin-resistant S. aureus, addition of oxacillin does not result in delocalization of PBP2 indicating that acylated PBP2 can be maintained in place by functional PBP2A, the central element of this resistance mechanism...
  11. pmc The peptidoglycan of stationary-phase Mycobacterium tuberculosis predominantly contains cross-links generated by L,D-transpeptidation
    Marie Lavollay
    INSERM, U872, LRMA, Equipe 12, F 75006 Paris, France
    J Bacteriol 190:4360-6. 2008
    ..tuberculosis beta-lactamases. Ldt(Mt1) and carbapenems may therefore represent a target and a drug family relevant to the eradication of persistent M. tuberculosis...
  12. ncbi Balance between two transpeptidation mechanisms determines the expression of beta-lactam resistance in Enterococcus faecium
    Jean Luc Mainardi
    INSERM EMI U 0004 Laboratoire de Recherche Moléculaire sur les Antibiotiques, UFR Broussais Hotel Dieu, Universite Paris VI, 75270 Paris, France
    J Biol Chem 277:35801-7. 2002
    ..The parallel dramatic increase in the proportion of l-Lys(3) --> d-Asx-Lys(3) cross-links showed that the enzyme was activating the resistance pathway by generating the substrate for the l,d-transpeptidase...
  13. ncbi The structural basis of ribosome activity in peptide bond synthesis
    P Nissen
    Department of Molecular Biophysics and Biochemistry and Department of Chemistry, Yale University, and Howard Hughes Medical Institute, New Haven, CT 06520 8114, USA
    Science 289:920-30. 2000
    ..The polypeptide exit tunnel is largely formed by RNA but has significant contributions from proteins L4, L22, and L39e, and its exit is encircled by proteins L19, L22, L23, L24, L29, and L31e...
  14. ncbi Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center
    Haripriya Ramu
    Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607, USA
    Mol Cell 41:321-30. 2011
    ..The extent of the conferred A-site selectivity is modulated by the C-terminal segment of the nascent peptide, where the third-from-last residue plays a critical role...
  15. pmc Resistance to the peptidyl transferase inhibitor tiamulin caused by mutation of ribosomal protein l3
    Jacob Bøsling
    Institute of Molecular Biology, University of Copenhagen, DK 1307 Copenhagen K, Denmark
    Antimicrob Agents Chemother 47:2892-6. 2003
    ..This is the first report of a mechanism of resistance to tiamulin unveiled in molecular detail...
  16. pmc Mutations of highly conserved bases in the peptidyltransferase center induce compensatory rearrangements in yeast ribosomes
    Rasa Rakauskaite
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    RNA 17:855-64. 2011
    ..the proto-ribosome area). We suggest that a certain degree of structural plasticity is built into the ribosome, enabling it to ensure accurate translation of the genetic code while providing it with the flexibility to adapt and evolve...
  17. pmc In vitro complementation analysis localizes 23S rRNA posttranscriptional modifications that are required for Escherichia coli 50S ribosomal subunit assembly and function
    R Green
    Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    RNA 2:1011-21. 1996
    ..Importantly, this chimeric reconstitution approach provides a system for the analysis of pure mutant populations of 23S rRNA reconstituted into 50S subunits...
  18. pmc Role of class A penicillin-binding proteins in PBP5-mediated beta-lactam resistance in Enterococcus faecalis
    Ana Arbeloa
    INSERM E0004 LRMA, Universite Paris VI, 75270 Paris, France
    J Bacteriol 186:1221-8. 2004
    ..The latter enzyme was not inhibited by moenomycin, since deletion of the three class A PBP genes led to high-level resistance to this glycosyltransferase inhibitor...
  19. ncbi Catalysis of ribosomal translocation by sparsomycin
    Kurt Fredrick
    Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA
    Science 300:1159-62. 2003
    ..These findings directly implicate the peptidyl transferase center of the 50S subunit in the mechanism of translocation, a process involving large-scale movement of tRNA and mRNA in the 30S subunit, some 70 angstroms away...
  20. ncbi Novel mechanism of beta-lactam resistance due to bypass of DD-transpeptidation in Enterococcus faecium
    J L Mainardi
    L R M A, UFR Broussais Hotel Dieu, Universite Paris VI, 75270 Paris, France
    J Biol Chem 275:16490-6. 2000
    ..The discovery of this novel resistance mechanism indicates that DD-transpeptidases cannot be considered anymore as the sole essential transpeptidase enzymes...
  21. pmc Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis
    B G Spratt
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
    Proc Natl Acad Sci U S A 86:8988-92. 1989
    ..flavescens, or a very similar commensal Neisseria species, appears to have occurred independently during the development of altered penA genes in non-beta-lactamase-producing penicillin-resistant strains of N. gonorrhoeae...
  22. ncbi EFG-independent translocation of the mRNA:tRNA complex is promoted by modification of the ribosome with thiol-specific reagents
    Daniel R Southworth
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD 21205, USA
    J Mol Biol 324:611-23. 2002
    ..These data suggest that molecular targets (ribosomal proteins) in the interface region of the ribosome are critical barriers that influence the translocation of the mRNA:tRNA complex...
  23. ncbi Structural basis for translation termination on the 70S ribosome
    Martin Laurberg
    Department of Molecular, Cell and Developmental Biology and Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Nature 454:852-7. 2008
    ..Unexpectedly, the main-chain amide group of Gln 230 in the universally conserved GGQ motif of the factor is positioned to contribute directly to peptidyl-tRNA hydrolysis...
  24. pmc Reduced heat resistance of mutant spores after cloning and mutagenesis of the Bacillus subtilis gene encoding penicillin-binding protein 5
    J A Todd
    J Bacteriol 167:257-64. 1986
    ..Preliminary chemical analysis indicated that the structure of the cortex of the mutant was different...
  25. pmc Targeting the cell wall of Mycobacterium tuberculosis: structure and mechanism of L,D-transpeptidase 2
    Sabri B Erdemli
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Structure 20:2103-15. 2012
    ..Together, this information provides vital insights to facilitate development of drugs targeting this validated yet unexploited enzyme...
  26. pmc Effect of polyamines on the inhibition of peptidyltransferase by antibiotics: revisiting the mechanism of chloramphenicol action
    Maria A Xaplanteri
    Laboratory of Biochemistry, School of Medicine, University of Patras, GR 26500 Patras, Greece
    Nucleic Acids Res 31:5074-83. 2003
    ..This fact may explain the effect of spermine on chloramphenicol binding to ribosomes...
  27. pmc Optimization of ribosome structure and function by rRNA base modification
    Jennifer L Baxter Roshek
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America
    PLoS ONE 2:e174. 2007
    ..This work investigates how ribosome structure and function are affected by rRNA base modification. The prevailing view is that rRNA base modifications serve to fine tune ribosome structure and function...
  28. pmc A mother cell-specific class B penicillin-binding protein, PBP4b, in Bacillus subtilis
    Yuping Wei
    Department of Biology, Virginia Tech, Blacksburg, Virginia 24061, USA
    J Bacteriol 186:258-61. 2004
    ..Loss of PBP4b, alone and in combination with other sporulation-specific PBPs, had no effect on spore peptidoglycan structure...
  29. ncbi Statics of the ribosomal exit tunnel: implications for cotranslational peptide folding, elongation regulation, and antibiotics binding
    Simone Fulle
    Department of Biological Sciences, Molecular Bioinformatics Group, Goethe University, Frankfurt, Germany
    J Mol Biol 387:502-17. 2009
    ..In order to explain antibiotics selectivity, action, and resistance, according to these results, differences in the degrees of freedom of the binding regions may need to be considered...
  30. pmc The interaction between C75 of tRNA and the A loop of the ribosome stimulates peptidyl transferase activity
    Julie L Brunelle
    Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 N Wolfe Street, 702A PCTB, Baltimore, MD 21205, USA
    RNA 12:33-9. 2006
    ....
  31. ncbi Growth and division of Streptococcus pneumoniae: localization of the high molecular weight penicillin-binding proteins during the cell cycle
    Cecile Morlot
    Institut de Biologie Structurale J P Ebel CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Mol Microbiol 50:845-55. 2003
    ..pneumoniae...
  32. pmc Structural basis for the inhibition of Mycobacterium tuberculosis L,D-transpeptidase by meropenem, a drug effective against extensively drug-resistant strains
    Hyoun Sook Kim
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Acta Crystallogr D Biol Crystallogr 69:420-31. 2013
    ..This work will facilitate structure-guided discovery of L,D-transpeptidase inhibitors as novel antituberculosis drugs against drug-resistant Mtb...
  33. pmc Localization of PBP3 in Caulobacter crescentus is highly dynamic and largely relies on its functional transpeptidase domain
    Teresa Costa
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Mol Microbiol 70:634-51. 2008
    ..Collectively, our results suggest a role for PBP3 in pole morphogenesis and provide new insights into the process of peptidoglycan assembly during division...
  34. ncbi The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis
    R A Daniel
    Sir William Dunn School of Pathology, University of Oxford, U K
    J Mol Biol 235:209-20. 1994
    ..The results support the proposition that spore cortex formation is determined primarily by the mother cell...
  35. ncbi Dynamics induced by β-lactam antibiotics in the active site of Bacillus subtilis L,D-transpeptidase
    Lauriane Lecoq
    CEA, DSV, Institut de Biologie Structurale IBS, Grenoble F 38027, France
    Structure 20:850-61. 2012
    ..The chemical step of the reaction determines enzyme specificity since no differences in drug affinity were observed...
  36. pmc Clostridium difficile has an original peptidoglycan structure with a high level of N-acetylglucosamine deacetylation and mainly 3-3 cross-links
    Johann Peltier
    Laboratoire G R A M, EA 2656 IFR 23, Rouen University Hospital, 22 Boulevard Gambetta, 76183 Rouen Cedex, France
    J Biol Chem 286:29053-62. 2011
    ..The contribution of 3-3 cross-links to peptidoglycan synthesis increased in the presence of ampicillin, indicating that this drug does not inhibit the L,D-transpeptidation pathway in C. difficile...
  37. pmc The contribution of metal ions to the structural stability of the large ribosomal subunit
    Daniel J Klein
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Ave, New Haven, CT 06520 8114, USA
    RNA 10:1366-79. 2004
    ....
  38. pmc Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions
    Maria A Xaplanteri
    Laboratory of Biochemistry, School of Medicine, University of Patras GR 26500 Patras, Greece
    Nucleic Acids Res 33:2792-805. 2005
    ..However, they exhibited higher reactivity toward puromycin and enhanced tRNA-translocation efficiency. These results suggest an essential role for polyamines in the structural and functional integrity of the large ribosomal subunit...
  39. pmc Bacillus subtilis cells lacking penicillin-binding protein 1 require increased levels of divalent cations for growth
    T Murray
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Bacteriol 180:4555-63. 1998
    ....
  40. ncbi Complex relationship between acquisition of beta-lactam resistance and loss of virulence in Streptococcus pneumoniae
    V Rieux
    Institut National de la Sante et de la Recherche Medicale, Hopital Bichat Claude Bernard, Paris, France
    J Infect Dis 184:66-72. 2001
    ..2b and 23.2b.2x mutants had no change in MIC. Compensatory mutations are implicated in the revival of virulence...
  41. ncbi Molecular mechanism of drug-dependent ribosome stalling
    Nora Vazquez-Laslop
    Center for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 South Ashland Avenue, M C 870, Chicago, IL 60607, USA
    Mol Cell 30:190-202. 2008
    ..The cladinose-containing macrolide antibiotic in the tunnel positions the nascent peptide for interaction with the tunnel sensory elements...
  42. pmc Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
    U Ilangovan
    Department of Chemistry and Biochemistry and University of California at Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 98:6056-61. 2001
    ..The structure suggests a mechanistic relationship to the papain/cathepsin proteases and should facilitate the design of new antiinfective agents...
  43. ncbi Ribosome structure and activity are altered in cells lacking snoRNPs that form pseudouridines in the peptidyl transferase center
    Thomas H King
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Mol Cell 11:425-35. 2003
    ..The possibility that modifying snoRNPs might affect ribosome structure in other ways is also discussed...
  44. ncbi The ribosomal peptidyl transferase
    Malte Beringer
    Institute of Physical Biochemistry, University of Witten Herdecke, Witten, Germany
    Mol Cell 26:311-21. 2007
    ..Proton transfer during the reaction appears to be promoted by a concerted proton shuttle mechanism that involves ribose hydroxyl groups on the tRNA substrate...
  45. pmc Peptidyl-transferase inhibitors have antiviral properties by altering programmed -1 ribosomal frameshifting efficiencies: development of model systems
    J D Dinman
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 94:6606-11. 1997
    ..Our results offer a new set of antiviral agents that may potentially have a broad range of applications in the clinical, veterinary, and agricultural fields...
  46. ncbi A novel peptidoglycan cross-linking enzyme for a beta-lactam-resistant transpeptidation pathway
    Jean Luc Mainardi
    INSERM, U655 Laboratoire de Recherche Moléculaire sur les Antibiotiques, Universite Pierre et Marie Curie, Paris, France
    J Biol Chem 280:38146-52. 2005
    ..Ldt(fm) homologues are encountered sporadically among taxonomically distant bacteria, indicating that ld-transpeptidase-mediated resistance may emerge in various pathogens...
  47. ncbi The D,D-carboxypeptidase PBP3 organizes the division process of Streptococcus pneumoniae
    Cecile Morlot
    Laboratoire d Ingénierie des Macromolécules, Institut de Biologie Structurale J P Ebel CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Mol Microbiol 51:1641-8. 2004
    ..Our work reveals an unexpected complexity in the relationships between the division proteins. The consequences of the absence of PBP3 indicate that the peptidoglycan composition is central to the co-ordination of the division process...
  48. ncbi Substrate-assisted catalysis of peptide bond formation by the ribosome
    Joshua S Weinger
    Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    Nat Struct Mol Biol 11:1101-6. 2004
    ..These results suggest that substrate assistance has been retained as a catalytic strategy during the evolution of the prebiotic peptidyl transferase center into the modern ribosome...
  49. pmc EF-G-independent reactivity of a pre-translocation-state ribosome complex with the aminoacyl tRNA substrate puromycin supports an intermediate (hybrid) state of tRNA binding
    Divya Sharma
    Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    RNA 10:102-13. 2004
    ..These data establish that pre-translocation-state ribosomes must sample or reside in an intermediate state of tRNA binding independent of the action of EF-G...
  50. pmc rRNA mutants in the yeast peptidyltransferase center reveal allosteric information networks and mechanisms of drug resistance
    Rasa Rakauskaite
    Department of Cell Biology and Molecular Genetics, University of Maryland, 2135 Microbiology Building, College Park, MD 20742, USA
    Nucleic Acids Res 36:1497-507. 2008
    ..These studies also add to our understanding of how information is transmitted both locally and over long distances through allosteric networks of rRNA-rRNA and rRNA-protein interactions...
  51. pmc Structure/function analysis of yeast ribosomal protein L2
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, Microbiology Building Rm 2135, University of Maryland, College Park, MD, 20742, USA
    Nucleic Acids Res 36:1826-35. 2008
    ....
  52. pmc Modulating the activity of the peptidyl transferase center of the ribosome
    Malte Beringer
    Institute of Physical Biochemistry, University of Witten Herdecke, 58448 Witten, Germany
    RNA 14:795-801. 2008
    ..Thus, local and long-range conformational rearrangements can lead to changes in the reaction specificity and catalytic activity of the PT center...
  53. pmc Ribosomal protein L3 functions as a 'rocker switch' to aid in coordinating of large subunit-associated functions in eukaryotes and Archaea
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, Microbiology Building Rm 2135, University of Maryland, College Park, MD 20742, USA
    Nucleic Acids Res 36:6175-86. 2008
    ..A model is presented describing how all three domains of L3 may function together as a 'rocker switch' to coordinate the stepwise processes of translation elongation...
  54. pmc Two class A high-molecular-weight penicillin-binding proteins of Bacillus subtilis play redundant roles in sporulation
    D C McPherson
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    J Bacteriol 183:6046-53. 2001
    ....
  55. pmc Identification, purification, and characterization of transpeptidase and glycosyltransferase domains of Streptococcus pneumoniae penicillin-binding protein 1a
    A M Di Guilmi
    Institut de Biologie Structurale Jean Pierre Ebel CEA CNRS, 38027 Grenoble Cedex 1, France
    J Bacteriol 180:5652-9. 1998
    ..The GT domain (Ser 37 to Lys 263) was expressed as a recombinant GST fusion protein. Protection by moenomycin of the GT domain against trypsin degradation was interpreted as an interaction between the GT domain and the moenomycin...
  56. pmc Labeling the peptidyltransferase center of the Escherichia coli ribosome with photoreactive tRNA(Phe) derivatives containing azidoadenosine at the 3' end of the acceptor arm: a model of the tRNA-ribosome complex
    J Wower
    Department of Biochemistry, University of Massachusetts, Amherst 01003
    Proc Natl Acad Sci U S A 86:5232-6. 1989
    ..coli ribosome...
  57. pmc Delayed rRNA processing results in significant ribosome biogenesis and functional defects
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    Mol Cell Biol 23:1602-13. 2003
    ..The frameshifting defect is accentuated when the demand for ribosomes is highest, suggesting that rRNA posttranscriptional modification is the bottleneck in ribosome biogenesis...
  58. ncbi Role of penicillin-binding proteins in bacterial cell morphogenesis
    David L Popham
    Department of Biology, Virginia Tech, Blacksburg, VA 24061, USA
    Curr Opin Microbiol 6:594-9. 2003
    ..In addition, the low molecular weight PBPs, by varying the substrates on which other PBPs act, alter peptidoglycan synthesis or turnover, with profound effects on morphology...
  59. pmc Alterations at the peptidyl transferase centre of the ribosome induced by the synergistic action of the streptogramins dalfopristin and quinupristin
    Jörg M Harms
    Max Planck Research Unit for Ribosomal Structure, 22603 Hamburg, Germany
    BMC Biol 2:4. 2004
    ..The streptogramins A display a prolonged activity that even persists after removal of the drug. However, the mode of activity of the streptogramins has not yet been fully elucidated, despite a plethora of biochemical and structural data...
  60. pmc An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination
    Melanie Amort
    Innsbruck Biocenter, Medical University Innsbruck, Division of Genomics and RNomics, Fritz Pregl Strasse 3, 6020 Innsbruck, Austria
    Nucleic Acids Res 35:5130-40. 2007
    ..These findings underscore the exceptional functional importance of the ribose moiety at A2602 for triggering peptide release...
  61. ncbi Mutations at nucleotides G2251 and U2585 of 23 S rRNA perturb the peptidyl transferase center of the ribosome
    R Green
    Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    J Mol Biol 266:40-50. 1997
    ....
  62. ncbi Protein synthesis at atomic resolution: mechanistics of translation in the light of highly resolved structures for the ribosome
    Daniel N Wilson
    Max Planck Institut fur Molekulare Genetik, AG Ribosomen, Ihnestr 73, Berlin, D 14195 Berlin, Germany
    Curr Protein Pept Sci 3:1-53. 2002
    ..Here we try a systematic synopsis of these ribosomal functions in light of the cryo-electron microscopic structures (resolution >7 A) and the atomic x-ray structures (>2.4 A) of the ribosome...
  63. ncbi Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide
    N Polacek
    Center for Pharmaceutical Biotechnology MC 870, University of Illinois, Chicago, Illinois 60607, USA
    Nature 411:498-501. 2001
    ....
  64. pmc Ribosomal protein L3 mutants alter translational fidelity and promote rapid loss of the yeast killer virus
    S W Peltz
    The Cancer Institute of New Jersey, Piscataway, New Jersey 08854, USA
    Mol Cell Biol 19:384-91. 1999
    ..Taken together, these results support the hypothesis that alterations in the peptidyltransferase center affect programmed -1 ribosomal frameshifting...
  65. ncbi Ribosome structure and the mechanism of translation
    V Ramakrishnan
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 108:557-72. 2002
    ..This review is an attempt to correlate the structures with biochemical and genetic data to identify the gaps and limits in our current knowledge of the mechanisms involved in translation...
  66. pmc Decreased peptidyltransferase activity correlates with increased programmed -1 ribosomal frameshifting and viral maintenance defects in the yeast Saccharomyces cerevisiae
    Arturas Meskauskas
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    RNA 9:982-92. 2003
    ....
  67. ncbi A conserved base-pair between tRNA and 23 S rRNA in the peptidyl transferase center is important for peptide release
    Jason S Feinberg
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093 0314, USA
    J Mol Biol 364:1010-20. 2006
    ....
  68. ncbi How ribosomes make peptide bonds
    Marina V Rodnina
    Institute of Physical Biochemistry, University of Witten Herdecke, D 58448 Witten, Germany
    Trends Biochem Sci 32:20-6. 2007
    ..Proton transfer during the reaction seems to be promoted by a concerted shuttle mechanism that involves ribose hydroxyl groups on the tRNA substrate...
  69. ncbi Instruction of translating ribosome by nascent peptide
    Feng Gong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 297:1864-7. 2002
    ..The location of tryptophan-12 of nascent TnaC in the peptide exit tunnel was crucial for induction. These results show that a nascent peptide sequence can influence translation continuation and termination within a translating ribosome...
  70. pmc Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression
    D Vinella
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universite Paris 7, France
    J Bacteriol 175:6704-10. 1993
    ..The plasmid pAQ, in which the ftsZ gene is inactivated, did not confer amdinocillin resistance. These results lead us to hypothesize that the nucleotide ppGpp activates ftsZ expression and thus couples cell division to protein synthesis...
  71. ncbi The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall
    Yinong Zong
    Center for Biophysical Sciences and Engineering, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Structure 12:105-12. 2004
    ....
  72. pmc A structural view on the mechanism of the ribosome-catalyzed peptide bond formation
    Miljan Simonovic
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, MBRB 1170, 900 S Ashland Ave, Chicago, IL 60607, USA
    Biochim Biophys Acta 1789:612-23. 2009
    ..A current understanding of the mechanism of the ribosome-catalyzed peptide bond formation is the focus of this review. Implications on the mechanism of peptide release are discussed as well...
  73. pmc Rapid peptide bond formation on isolated 50S ribosomal subunits
    Ingo Wohlgemuth
    Institute of Physical Biochemistry, University of Witten Herdecke, Stockumer Strasse 10, 58448 Witten, Germany
    EMBO Rep 7:699-703. 2006
    ....
  74. ncbi Structural insights into the roles of water and the 2' hydroxyl of the P site tRNA in the peptidyl transferase reaction
    T Martin Schmeing
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 20:437-48. 2005
    ....
  75. pmc Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit
    J Thompson
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Proc Natl Acad Sci U S A 98:9002-7. 2001
    ..The significant levels of peptidyltransferase activity of ribosomes with mutations at A2451 and G2447 need to be reconciled with the roles proposed for these residues in catalysis...
  76. ncbi The ribosomal peptidyl transferase center: structure, function, evolution, inhibition
    Norbert Polacek
    Innsbruck Biocenter, Division of Genomics and RNomics, Innsbruck Medical University, Innsbruck, Austria
    Crit Rev Biochem Mol Biol 40:285-311. 2005
    ..Thus, it came as a surprise that recent findings revealed an unexpected high level of variation in the mode of antibiotic binding to the PTC of ribosomes from different organisms...
  77. pmc NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center
    S Wang
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    J Cell Biol 130:519-28. 1995
    ..Since NAC is not a core ribosomal protein, the emergence of nascent chains from the ribosome may be more dynamic than previously thought...
  78. pmc FemABX peptidyl transferases: a link between branched-chain cell wall peptide formation and beta-lactam resistance in gram-positive cocci
    S Rohrer
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Antimicrob Agents Chemother 47:837-46. 2003
  79. pmc Mononucleotide derivatives as ribosomal P-site substrates reveal an important contribution of the 2'-OH to activity
    Silke Dorner
    Max F Perutz Laboratories, University Department at the Vienna Biocenter, Institut für Med Biochemie, Universitat Wien, Dr Bohrgasse 9 3, A 1030 Wien, Austria
    Nucleic Acids Res 31:6536-42. 2003
    ..A model is discussed how further interactions of the 2'-OH in the transition state might influence peptidyl transferase activity...
  80. ncbi Base-pairing between 23S rRNA and tRNA in the ribosomal A site
    D F Kim
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell 4:859-64. 1999
    ..Genetic analysis in vitro of U2552 and G2553 mutant ribosomes and CCA end mutant tRNA substrates identified a base-pairing interaction between C75 of aminoacyl tRNA and G2553 of 23S rRNA...
  81. pmc Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes
    Ashley Eversole Hesslein
    Yale University, Department of Molecular Biophysics and Biochemistry, 260 Whitney Avenue, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 32:3760-70. 2004
    ....
  82. ncbi Historical review: Peptidyl transfer, the Monro era
    B Edward H Maden
    School of Biological Sciences, University of Liverpool, Biosciences Building, Liverpool, L69 7ZB, UK
    Trends Biochem Sci 28:619-24. 2003
    ..Monro's 'fragment reaction', the ribosome catalyzed reaction of a fragment of formylmethionyl-tRNA with puromycin, remains in use in work on peptidyl transfer...
  83. pmc Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center
    O Dontsova
    Department of Chemistry, Moscow State University, Russia
    Proc Natl Acad Sci U S A 91:4125-9. 1994
    ..On the basis of this result, and in combination with earlier immunoelectron microscopic data, we propose a model for the orientation of the 5S RNA in the 50S subunit...
  84. ncbi Ribosomal protein L27 participates in both 50 S subunit assembly and the peptidyl transferase reaction
    I K Wower
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003 4505, USA
    J Biol Chem 273:19847-52. 1998
    ..We therefore infer that L27 contributes to peptide bond formation by facilitating the proper placement of the acceptor end of the A-site tRNA at the peptidyl transferase center...
  85. ncbi A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome
    R R Samaha
    Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    Nature 377:309-14. 1995
    ..These findings establish a direct role for 23S rRNA in protein synthesis...
  86. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
    ....
  87. pmc Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer
    G Diedrich
    Max Planck Institut fur Molekulare Genetik, AG Ribosomen, Ihnestrasse 73, D 14195 Berlin, Germany
    EMBO J 19:5241-50. 2000
    ..These results are used to examine critically the proposed direct involvement of His229 in catalysis of peptide synthesis...
  88. ncbi On the structural basis of peptide-bond formation and antibiotic resistance from atomic structures of the large ribosomal subunit
    Thomas A Steitz
    Department of Molecular Biophysics and Biochemistry, Yale University, and Howard Hughes Medical Institute, P O Box 208114, New Haven, CT 06520 8114, USA
    FEBS Lett 579:955-8. 2005
    ..The structure shows that the ribosome is indeed a ribozyme...
  89. pmc Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451
    Matthias D Erlacher
    Innsbruck Biocenter, Division of Genomics and RNomics Innsbruck Medical University Fritz Pregl Strasse 3, 6020 Innsbruck, Austria
    Nucleic Acids Res 33:1618-27. 2005
    ..This implicates a functional or structural role of the 2'-hydroxyl group at A2451 for transpeptidation...
  90. ncbi A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome
    Bruce A Maguire
    Department of Biochemistry and Molecular Biology, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA
    Mol Cell 20:427-35. 2005
    ....
  91. ncbi Effects of two photoreactive spermine analogues on peptide bond formation and their application for labeling proteins in Escherichia coli functional ribosomal complexes
    I Amarantos
    Laboratory of Biochemistry, School of Medicine, University of Patras, GR-26500 Patras, Greece
    Biochemistry 40:7641-50. 2001
    ..On the basis of these results and literature data, the involvement of spermine in the conformation and important functions of ribosomes is discussed...
  92. pmc Ribosomal protein L5 helps anchor peptidyl-tRNA to the P-site in Saccharomyces cerevisiae
    A Meskauskas
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway 08854, USA
    RNA 7:1084-96. 2001
    ..A model is presented describing how decreased affinity of ribosomes for peptidyl-tRNA can affect both -1 and +1 frameshifting, and for the effects of sparsomycin...
  93. ncbi The social life of ribosomal proteins
    Ditlev E Brodersen
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Denmark
    FEBS J 272:2098-108. 2005
    ..In this review we highlight some of the many known and important functions of ribosomal proteins, both during translation on the ribosome and in a wider context...
  94. ncbi Yeast ribosomal protein deletion mutants possess altered peptidyltransferase activity and different sensitivity to cycloheximide
    J Dresios
    Laboratory of Biochemistry, School of Medicine, University of Patras, 26110 Patras, Greece
    Biochemistry 40:8101-8. 2001
    ..Translocation of Ac-Phe-tRNA from the A- to P-site was inhibited by 50% at 1.4 microM cycloheximide for the L24 mutant compared to 0.7 microM for the wild type...
  95. ncbi Important contribution to catalysis of peptide bond formation by a single ionizing group within the ribosome
    Vladimir I Katunin
    Sankt Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russia
    Mol Cell 10:339-46. 2002
    ..These results suggest a contribution to overall catalysis of general acid-base and/or conformational catalysis involving an ionizing group at the active site...
  96. pmc Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome
    Rebecca M Voorhees
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nat Struct Mol Biol 16:528-33. 2009
    ..They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer...
  97. ncbi Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2'-OH at A2451 of 23S rRNA
    Matthias D Erlacher
    Innsbruck Biocenter, Division of Genomics and RNomics Innsbruck Medical University, Austria
    J Am Chem Soc 128:4453-9. 2006
    ..These data highlight the unique functional role of the A2451 2'-OH for peptide bond synthesis among all other functional groups at the ribosomal peptidyl transferase active site...
  98. pmc An arc of unpaired "hinge bases" facilitates information exchange among functional centers of the ribosome
    Rasa Rakauskaite
    Department of Cell Biology and Molecular Genetics, University of Maryland, 2135 Microbiology Building, College Park, MD 20742, USA
    Mol Cell Biol 26:8992-9002. 2006
    ..We propose that a series of regularly spaced "hinge bases" provide fulcrums around which rigid helices can reorient themselves depending on the occupancy status of the A-site...
  99. pmc The A2453-C2499 wobble base pair in Escherichia coli 23S ribosomal RNA is responsible for pH sensitivity of the peptidyltransferase active site conformation
    Mark A Bayfield
    Department of Molecular Biology, Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Nucleic Acids Res 32:5512-8. 2004
    ..coli peptidyltransferase center, its lack of conservation makes it, and consequently its near-neutral pK(a), unlikely to contribute to function during peptide bond formation...
  100. ncbi Unusual resistance of peptidyl transferase to protein extraction procedures
    H F Noller
    Sinsheimer Laboratories, University of California, Santa Cruz
    Science 256:1416-9. 1992
    ..In contrast, activity is abolished by treatment with ribonuclease T1. These findings support the possibility that 23S ribosomal RNA participates in the peptidyl transferase function...
  101. ncbi The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination
    Norbert Polacek
    Center for Pharmaceutical Biotechnology m c 870, University of Illinois, 900 S Ashland Avenue, Chicago, IL 60607, USA
    Mol Cell 11:103-12. 2003
    ..This indicates that the mechanism of peptide release is distinct from that of peptide bond formation, with A2602 playing a critical role in peptide release during translation termination...

Research Grants22

  1. CRYSTALLOGRAPHIC STUDIES ON RIBOSOMAL PARTICLES
    ADA E YONATH; Fiscal Year: 2010
    ..These studies have already illuminated principles of drug selectivity, and provided significant basic knowledge of possible pathways of drug resistance, hence paving the way to improve therapeutic properties. ..
  2. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
    ..abstract_text> ..
  3. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
    ....
  4. NMR Console Upgrade and Cryoprobe
    Peter Moore; Fiscal Year: 2002
    ..There are several structural biology projects under consideration that demand this sensitivity because the molecules of interest aggregate at the concentrations required when standard probes are used. ..
  5. STRUCTURE AND PHYSICAL PROPERTIES OF RNA
    Peter Moore; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  6. RIBOSOME STRUCTURE/FUNCTION
    Peter Moore; Fiscal Year: 2001
    ..The structures of all new crystals obtained that are suitable for crystallographic analysis will be pursued. Third, the crustal structure of elongation factor G (EF-G) complexed with a GTP analogue will be solved. ..
  7. Upgrade of X-Ray Diffractometers
    Peter Moore; Fiscal Year: 2005
    ..It is anticipated that this upgrade will significantly increase the rate at which structures are determined at Yale. ..
  8. REVERSE TRANSCRIPTASE AND OTHER HIV MOLECULAR STRUCTURES
    Thomas Steitz; Fiscal Year: 2002
    ..Crystallographic studies of both full length HIV integrase and the homologous transposase, TN552, complexed with suitable DNA substrates are aimed to produce mechanistic insights. ..
  9. Structural studies on ribosomes and antibiotics
    VENKATRAMAN RAMAKRISHNAN; Fiscal Year: 2006
    ..Such knowledge could help improve existing antibiotics and could also lead to the design of new ones. ..
  10. Characterization of the SARSCoV frameshift signal
    Jonathan D Dinman; Fiscal Year: 2010
    ....
  11. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..Together, these projects utilize genetic, biochemical, biophysical, and proteomics approaches to generate new insights into the mechanisms of antibiotic action in N. gonorrhoeae. ..
  12. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2010
    ..The proposed work is to study how resistance to beta-lactam antibiotics has developed. The understanding of the mechanistic details should guide the way in devising strategies to circumvent the problem. ..
  13. FORMATION AND FUNCTION OF YEAST MITOCHONDRIAL RIBOSOMES
    Thomas Mason; Fiscal Year: 2002
    ....
  14. NASCENT PEPTIDE CONTROL OF THE RIBOSOME
    PAUL LOVETT; Fiscal Year: 2001
    ..We have identified a simple method that should allow determination of the critical amino acids for binding. Further, we will refine the identification of the rRNA site for peptide binding by high resolution footprinting. ..
  15. ASSEMBLY OF THE DIVISION SEPTUM IN ESCHERICHIA COLI
    David Weiss; Fiscal Year: 2003
    ..In this regard, it is worth noting that four of the proteins to be studied here (FtsI and the three transglycosylases) are primary targets of beta-lactam antibiotics. ..
  16. Regulation of gene expression by ribosomal frameshifting
    JONATHAN DINMAN; Fiscal Year: 2004
    ....
  17. PREVENTION OF PNEUMOCOCCAL DISEASE IN HIV INFECTION
    MARY MCELLISTREM; Fiscal Year: 2004
    ..All patients who exhibit a greater that 3-fold transient rise in HIV-1 RNA levels 2 and 4 weeks postimmunization will be evaluated by Fisher's Exact and McNemar's Tests. ..
  18. RIBOSOMAL FRAMESHIFTING AS A PROBE OF 5S RRNA FUNCTION
    JONATHAN DINMAN; Fiscal Year: 2004
    ..The proposed research will serve to further our understanding of this ubiquitous yet little understood rRNA and will place us in the unique position to link functional aspects of 5S rRNA to its structure within the ribosome. ..
  19. Acquisition of a 600 MHz NMR Spectrometer - Wayne State
    Shahriar Mobashery; Fiscal Year: 2002
    ..The availability of this instrument would stimulate further research in structural biology at Wayne State University, enhancing the strong existing capabilities that we enjoy in X-ray structure determination. ..
  20. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..
  21. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
    ..Our studies will provide novel and important information on the cellular mechanisms of P2Y receptor targeting and the regulation of P2Y receptor signaling activity in epithelial cells. ..
  22. RIBOSOMAL FUNCTION & ANTIBIOTIC DESIGN
    Shahriar Mobashery; Fiscal Year: 2007
    ..This collective effort will shed light both on the various molecular events within the ribosomal and on how to inhibit some of these processes. [unreadable] [unreadable]..