chloramphenicol o acetyltransferase

Summary

Summary: An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.

Top Publications

  1. ncbi Transcription of the human immunodeficiency virus type 1 (HIV-1) promoter in central nervous system cells: effect of YB-1 on expression of the HIV-1 long terminal repeat
    B E Sawaya
    Center for NeuroVirology and NeuroOncology, Department of Neurology, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA
    J Gen Virol 79:239-46. 1998
  2. ncbi Anticodon sequence mutants of Escherichia coli initiator tRNA: effects of overproduction of aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase, and initiation factor 2 on activity in initiation
    Christine Mayer
    Department of Biology, 68 671 Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 42:4787-99. 2003
  3. pmc Analysis of fluorescent protein expression in transformants of Rickettsia monacensis, an obligate intracellular tick symbiont
    Gerald D Baldridge
    Department of Entomology, University of Minnesota, 1980 Folwell Ave, St Paul, MN 55108, USA
    Appl Environ Microbiol 71:2095-105. 2005
  4. ncbi Entamoeba histolytica: structural and functional analysis of the Ehadh112 gene promoter
    E Azuara-Liceaga
    Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, San Lorenzo 290, Col del Valle, CP 03100, Mexico D F, Mexico
    Exp Parasitol 110:280-5. 2005
  5. ncbi Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation
    A A Komar
    Centre de Genetique Moleculaire, CNRS, Gif sur Yvette, France
    FEBS Lett 462:387-91. 1999
  6. ncbi Entamoeba histolytica: functional characterization of the -234 to -196 bp promoter region of the multidrug resistance EhPgp1 gene
    M Esther Ramirez
    Programa Institucional de Biomedicina Molecular, ENMyH IPN, Guillermo Massieu Helguera, No 239 Fracc La Escalera, Ticoman, CP 07320 Mexico, D F, Mexico
    Exp Parasitol 110:238-43. 2005
  7. pmc Ribosome-initiator tRNA complex as an intermediate in translation initiation in Escherichia coli revealed by use of mutant initiator tRNAs and specialized ribosomes
    X Q Wu
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    EMBO J 15:4734-9. 1996
  8. ncbi CpG-depleted plasmid DNA vectors with enhanced safety and long-term gene expression in vivo
    Nelson S Yew
    Genzyme Corporation, 31 New York Avenue, Framingham, Massachusetts 01701 9322, USA
    Mol Ther 5:731-8. 2002
  9. pmc Transcriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells
    V Camara-Clayette
    INSERM U76, Institut National de la Transfusion Sanguine, rue Alexandre Cabanel, 75015 Paris, France
    Biochem J 356:171-80. 2001
  10. ncbi Entamoeba histolytica EhPgp5 transcriptional activation depends on putative emetine response elements
    Alma Nieto
    Programa Institucional de Biomedicina Molecular, ENMyH IPN, Guillermo Massieu Helguera No 239 Fracc La Escalera Ticomán, CP 07320, Mexico, D F, Mexico
    Exp Parasitol 110:233-7. 2005

Research Grants

Detail Information

Publications227 found, 100 shown here

  1. ncbi Transcription of the human immunodeficiency virus type 1 (HIV-1) promoter in central nervous system cells: effect of YB-1 on expression of the HIV-1 long terminal repeat
    B E Sawaya
    Center for NeuroVirology and NeuroOncology, Department of Neurology, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA
    J Gen Virol 79:239-46. 1998
    ....
  2. ncbi Anticodon sequence mutants of Escherichia coli initiator tRNA: effects of overproduction of aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase, and initiation factor 2 on activity in initiation
    Christine Mayer
    Department of Biology, 68 671 Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 42:4787-99. 2003
    ..Other results suggest that the C1xA72 base pair mismatch, unique to eubacterial and organellar initiator tRNAs, may also be important for the binding of fAA-tRNA to IF2...
  3. pmc Analysis of fluorescent protein expression in transformants of Rickettsia monacensis, an obligate intracellular tick symbiont
    Gerald D Baldridge
    Department of Entomology, University of Minnesota, 1980 Folwell Ave, St Paul, MN 55108, USA
    Appl Environ Microbiol 71:2095-105. 2005
    ..monacensis. We demonstrated that our vectors provide a means to study rickettsia-host cell interactions by visualizing GFPuv-fluorescent R. monacensis associated with actin tails in tick host cells...
  4. ncbi Entamoeba histolytica: structural and functional analysis of the Ehadh112 gene promoter
    E Azuara-Liceaga
    Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, San Lorenzo 290, Col del Valle, CP 03100, Mexico D F, Mexico
    Exp Parasitol 110:280-5. 2005
    ..Transcription initiation sites (-69 and -150 bp), TATA like box, GAAC, and Inr elements, as well as putative DNA binding motifs, were mapped in the -1 to -469 bp core promoter region...
  5. ncbi Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation
    A A Komar
    Centre de Genetique Moleculaire, CNRS, Gif sur Yvette, France
    FEBS Lett 462:387-91. 1999
    ..coli S30 extract system was shown to possess 20% lower specific activity. The data suggest that kinetics of protein translation can affect the in vivo protein-folding pathway, leading to increased levels of protein misfolding...
  6. ncbi Entamoeba histolytica: functional characterization of the -234 to -196 bp promoter region of the multidrug resistance EhPgp1 gene
    M Esther Ramirez
    Programa Institucional de Biomedicina Molecular, ENMyH IPN, Guillermo Massieu Helguera, No 239 Fracc La Escalera, Ticoman, CP 07320 Mexico, D F, Mexico
    Exp Parasitol 110:238-43. 2005
    ..Finally, we partially purified three proteins of 64.4, 56.7, and 27.4 kDa. Further investigations are currently in progress to determine the identity of these nuclear factors and how they are interacting with the EhPgp1 gene promoter...
  7. pmc Ribosome-initiator tRNA complex as an intermediate in translation initiation in Escherichia coli revealed by use of mutant initiator tRNAs and specialized ribosomes
    X Q Wu
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    EMBO J 15:4734-9. 1996
    ..In cells overproducing the mutant initiator tRNAs, most ribosomes would carry the mutant initiator tRNA and these ribosomes would select the mutant CAT mRNA over the other mRNAs...
  8. ncbi CpG-depleted plasmid DNA vectors with enhanced safety and long-term gene expression in vivo
    Nelson S Yew
    Genzyme Corporation, 31 New York Avenue, Framingham, Massachusetts 01701 9322, USA
    Mol Ther 5:731-8. 2002
    ..This combination of decreased toxicity and sustained expression suggests that CpG-depleted pDNA vectors can greatly improve the safety and efficacy of synthetic gene delivery systems...
  9. pmc Transcriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells
    V Camara-Clayette
    INSERM U76, Institut National de la Transfusion Sanguine, rue Alexandre Cabanel, 75015 Paris, France
    Biochem J 356:171-80. 2001
    ..Altogether, the results indicated that KEL expression is not restricted to erythroid tissue...
  10. ncbi Entamoeba histolytica EhPgp5 transcriptional activation depends on putative emetine response elements
    Alma Nieto
    Programa Institucional de Biomedicina Molecular, ENMyH IPN, Guillermo Massieu Helguera No 239 Fracc La Escalera Ticomán, CP 07320, Mexico, D F, Mexico
    Exp Parasitol 110:233-7. 2005
    ....
  11. ncbi The Entamoeba histolytica Ehcp112 gene has a distal and weak promoter
    E Flores-Soto
    Posgrado en Ciencias Genómicas de la Universidad Autónoma de la Ciudad de México, Mexico D F Ave San Lorenzo 290, Col del Valle, Mexico, D F 03100, Mexico
    Exp Parasitol 110:286-91. 2005
    ..Our results showed the presence of a distal and weak promoter in the Ehcp112 gene. The active DNA region is inside the open reading frame of the Ehrab B gene, suggesting that expression of both genes could be coordinately regulated...
  12. ncbi Transcriptional regulation of Kaposi's sarcoma-associated herpesvirus-encoded oncogene viral interferon regulatory factor by a novel transcriptional silencer, Tis
    Xin Ping Wang
    Departments of Pediatrics and Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 3900, USA
    J Biol Chem 277:12023-31. 2002
    ..These results define the function of a novel transcriptional silencer in the regulation of vIRF gene expression...
  13. ncbi A member of the Y-box protein family interacts with an upstream element in the alpha1(I) collagen gene
    W Sun
    Department of Biochemistry, Boston University School of Medicine, 715 Albany St, Boston, MA 02118, USA
    Matrix Biol 20:527-41. 2001
    ..YB-1 protein activates the collagen promoter and translocates into the nucleus during TGF-beta addition to fibroblasts, suggesting a role for this protein in TGF-beta signaling...
  14. ncbi Characterization of the response element and DNA binding properties of the nuclear orphan receptor germ cell nuclear factor/retinoid receptor-related testis-associated receptor
    Z H Yan
    Department of Medicine III, Osaka University Medical School, Yamadaoka, Suita, Osaka 565, Japan
    J Biol Chem 272:10565-72. 1997
    ..Transfection of RTR in CV-1 cells was unable to cause RTRE-dependent transactivation of a CAT reporter gene; however, an RTR-VP16 fusion protein could induce transactivation through several RTREs, including P2-RE...
  15. ncbi In vitro and in vivo promoter analyses of the mouse phospholamban gene
    K Haghighi
    Department of Pharmacology and Cell Biophysics, University of Cincinnati, College of Medicine, OH 45267 0575, USA
    Gene 203:199-207. 1997
    ....
  16. ncbi The pancreatic beta-cell-specific transcription factor Pax-4 inhibits glucagon gene expression through Pax-6
    Beate Ritz-Laser
    Diabetes Unit, Geneva University Hospital, 24, Rue Micheli du Crest, 1211 Geneva 14, Switzerland
    Diabetologia 45:97-107. 2002
    ....
  17. ncbi Promoter-specific activation and demethylation by MBD2/demethylase
    Nancy Detich
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 277:35791-4. 2002
    ..1His vector. We propose that a single protein possessing both repressor and demethylase functions has evolved to coordinate a program that requires suppression of some methylated genes and activation of others...
  18. ncbi Light chain promoter regions in rainbow trout Oncorhynchus mykiss: function of a classical and an atypical Ig promoter
    Sirje Timmusk
    Immunology Programme, Department of Cell and Molecular Biology, BMC, Uppsala University, Box 596, Husargatan 3, S 751 24 Uppsala, Sweden
    Dev Comp Immunol 26:785-96. 2002
    ..Both L1 and L2 promoters can cooperate with a B cell-specific enhancer from Atlantic cod (Gadus morhua)...
  19. ncbi Transcription of the beta-galactoside alpha 2,6-sialyltransferase gene in B lymphocytes is directed by a separate and distinct promoter
    N W Lo
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Glycobiology 6:271-9. 1996
    ..Louckes cells expressed CAT efficiently from Form 2 promoter but only marginally from the Form 1 promoter. In contrast, CAT expression from Form 1 promoter is more efficient than from the Form 2 promoter in HepG2 cells...
  20. ncbi The co-activator p300 associates physically with and can mediate the action of the distal enhancer of the FGF-4 gene
    Tamara Nowling
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198, USA
    J Biol Chem 278:13696-705. 2003
    ..Our results also suggest that intragenic distal enhancers may use mechanisms that differ from extragenic distal enhancers...
  21. ncbi Preliminary characterisation of mechanoresponsive regions of the c-fos promoter in bone cells
    Matthew A Peake
    Centre for Science and Technology in Medicine, School of Medicine, Keele University, North Staffordshire Hospital, Hartshill, Stoke on Trent, UK
    FEBS Lett 537:117-20. 2003
    ..These results suggest that the mechanical induction of c-fos in osteoblastic bone cells is mediated by multiple response elements...
  22. pmc The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes
    B Ohana
    Department of Gene Regulation, Roche Institute of Molecular Biology, Nutley, NJ 07110
    Proc Natl Acad Sci U S A 90:138-42. 1993
    ..This finding, together with the observation that TBP-1 does not activate each promoter examined, suggests that this gene family may encode promoter-specific transcriptional activators...
  23. ncbi ZNF143 mediates basal and tissue-specific expression of human transaldolase
    Craig E Grossman
    Department of Medicine, State University of New York, Upstate Medical University, College of Medicine, 750 East Adams Street, Syracuse, NY 13210, USA
    J Biol Chem 279:12190-205. 2004
    ..Thus, ZNF143 plays a key role in basal and tissue-specific expression of transaldolase and regulation of the metabolic network controlling cell survival and differentiation...
  24. ncbi Reactive oxygen species mediate cyclic strain-induced endothelin-1 gene expression via Ras/Raf/extracellular signal-regulated kinase pathway in endothelial cells
    T H Cheng
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC
    J Mol Cell Cardiol 33:1805-14. 2001
    ..Our data demonstrate that strain-induced Et-1 gene expression is modulated by ROS via Ras/Raf/ERK signaling pathway, and indicate the responsiveness of the AP-1 binding site for strain-induced Et-1 expression...
  25. ncbi Transcriptional regulation of the TFIIH transcription repair components XPB and XPD by the hepatitis B virus x protein in liver cells and transgenic liver tissue
    I Jaitovich-Groisman
    Lady Davis Institute of the Sir Mortimer B Davis Jewish General Hospital, Departments of Medicine, Pharmacology and Therapeutics, Pathology, and Oncology, Faculty of Medicine, McGill University, Montreal H3T 1E2, Canada
    J Biol Chem 276:14124-32. 2001
    ..In summary, our results provide evidence that HBx represses the expression of key TFIIH proteins at least in part through Sp1 elements; this repression may impair TFIIH function in DNA repair mechanisms...
  26. ncbi Epidermal growth factor induction of apolipoprotein A-I is mediated by the Ras-MAP kinase cascade and Sp1
    X L Zheng
    Endocrine Research Group, Departments of Medicine, University of Calgary, Alberta, Canada T2N 4N1
    J Biol Chem 276:13822-9. 2001
    ..However, insulin induction of this gene is different and requires both Ras-MAP kinase and PKC pathways but their actions are also mediated by Sp1...
  27. ncbi Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter
    C Lange
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092 Berlin, Germany
    Biochem Biophys Res Commun 278:125-33. 2000
    ..The promoter is TATA-less and contains an inverted CCAAT-box and a Sp1 site located near to a p53 binding motif. An alternative 3'-splice site of intron 1 results in a splicing variant within the 5'-untranslated region of MVP mRNA...
  28. ncbi Protein kinase A phosphorylates hepatocyte nuclear factor-6 and stimulates glucose-6-phosphatase catalytic subunit gene transcription
    R S Streeper
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA
    J Biol Chem 276:19111-8. 2001
    ..These results suggest that the stimulatory effect of PKA on G6Pase fusion gene transcription in HepG2 cells may be mediated in part by the phosphorylation of HNF-6...
  29. ncbi Regulation of transcription of the human presenilin-1 gene by ets transcription factors and the p53 protooncogene
    M Pastorcic
    Department of Pharmacology and Neuroscience and Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    J Biol Chem 275:34938-45. 2000
    ..We show that p53 does not bind directly to the PS1 promoter. Hence the repression of PS1 transcription by p53 is likely to be mediated through protein-protein interactions...
  30. ncbi Dexamethasone induces lipocalin-type prostaglandin D synthase gene expression in mouse neuronal cells
    L F Garcia-Fernandez
    Instituto de Investigaciones Biomédicas Alberto Sols Consejo Superior de Investigaciones Científicas Universidad Autónoma de Madrid, Madrid, Spain
    J Neurochem 75:460-70. 2000
    ..In contrast, neither dexamethasone nor TPA affected the expression of cyclooxygenases-1 and -2. Our data demonstrate that dexamethasone induces L-PGDS gene transcription in neuronal cells...
  31. ncbi Lung Krüppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase
    M D Conkright
    Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
    J Biol Chem 276:29299-306. 2001
    ....
  32. ncbi GATA-6 activates transcription of surfactant protein A
    M D Bruno
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 275:1043-9. 2000
    ..GATA-6 is expressed in respiratory epithelial cells and binds to a cis-acting element in the SP-A gene promoter, activating the transcriptional activity of the gene...
  33. pmc Transforming growth factor beta-1 enhances Smad transcriptional activity through activation of p8 gene expression
    A C Garcia-Montero
    INSERM U 315, 46 boulevard de la Gaye, F 13009 Marseille, France
    Biochem J 357:249-53. 2001
    ..We concluded that TGFbeta-1 activates p8 expression, which in turn enhances the Smad-transactivating function responsible for TGFbeta-1 activity...
  34. pmc Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex
    H Peled-Zehavi
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143, USA
    Mol Cell Biol 21:5232-41. 2001
    ..Using a homology model constructed from the cocrystal structure of a Nova KH domain-RNA complex (Lewis et al., Cell 100:323-332, 2000), we propose a plausible arrangement for SF1/mBBP-U2AF complexes assembled at 3' splice sites...
  35. ncbi Effect of the R1 element on expression of the US3 and US6 immune evasion genes of human cytomegalovirus
    G C Bullock
    Program in Molecular Biology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Virology 288:164-74. 2001
    ..The potential mechanisms of R1 enhancement of US3 and US6 gene expression are discussed...
  36. ncbi Characterization of the human CALM2 calmodulin gene and comparison of the transcriptional activity of CALM1, CALM2 and CALM3
    S L Toutenhoofd
    Department of Biochemistry and Molecular Biology, Mayo Graduate School, Mayo Foundation, Rochester, MN 55905, USA
    Cell Calcium 23:323-38. 1998
    ..We conclude that the CALM gene family is differentially active at the transcriptional level in teratoma cells and that the 5' untranslated regions are necessary to recover full promoter activation...
  37. ncbi The stage-regulated expression of Leishmania mexicana CPB cysteine proteases is mediated by an intercistronic sequence element
    D R Brooks
    Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 276:47061-9. 2001
    ..This is the first well defined cis-regulatory element implicated in post-transcriptional stage-specific gene expression in Leishmania...
  38. ncbi An enhancer region within the copia untranslated leader contains binding sites for Drosophila regulatory proteins
    S Wilson
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Gene 209:239-46. 1998
    ..Another Drosophila protein previously implicated in fat body specific expression of the alcohol dehydrogenase gene (Adh), the Box-B-binding factor-2 (BBF-2), is also shown to bind to the copia ULR...
  39. ncbi Upstream stimulating factor-1 (USF1) and USF2 bind to and activate the promoter of the adenomatous polyposis coli (APC) tumor suppressor gene
    A S Jaiswal
    Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    J Cell Biochem 81:262-77. 2001
    ..Thus, USF1 and USF2 transcription factors are critical for APC gene expression...
  40. ncbi OTX2 activates the molecular network underlying retina pigment epithelium differentiation
    Juan Ramon Martinez-Morales
    Instituto Cajal, Consejo Superior de Investigaciones Cientificas, Dr Arce 37, Madrid 28002, Spain
    J Biol Chem 278:21721-31. 2003
    ..Collectively, these data suggest that both transcription factors operate at the same hierarchical level to establish the identity of the RPE...
  41. pmc Effects of the human immunodeficiency virus type 1 Tat protein on the expression of inflammatory cytokines
    L Buonaguro
    Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892
    J Virol 66:7159-67. 1992
    ....
  42. ncbi HOXA9 activates transcription of the gene encoding gp91Phox during myeloid differentiation
    Ling Bei
    Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    J Biol Chem 280:12359-70. 2005
    ..These studies also suggest overexpression of Meis1 or Nup98-hoxA9 represses myeloid-specific gene transcription, thereby contributing to differentiation block in leukemogenesis...
  43. ncbi Hepatitis C virus core protein down-regulates transcription of interferon-induced antiviral genes
    Susana de Lucas
    Fundacion para el Estudio de las Hepatitis Virales, Madrid 28015, Spain
    J Infect Dis 191:93-9. 2005
    ..We analyzed the effect of HCV core (HCV-Co) protein on expression of IFN-induced antiviral genes...
  44. ncbi Essential interaction of Egr-1 at an islet-specific response element for basal and gastrin-dependent glucagon gene transactivation in pancreatic alpha-cells
    Stéphane Leung-Theung-Long
    INSERM U531, IFR31, Hospital Rangueil, 31059 Toulouse Cedex 9, France
    J Biol Chem 280:7976-84. 2005
    ..Moreover they open new working hypothesis with reference to a potential role of gastrin in glucagon-producing pancreatic cells...
  45. ncbi Transcriptional activity of the paired-like homeodomain proteins CHX10 and VSX1
    Kimberley M Dorval
    Toronto Western Research Institute, University Health Network Program, University of Toronto, Toronto, Ontario M5T 2S8, Canada
    J Biol Chem 280:10100-8. 2005
    ..Therefore, CHX10 and VSX1 may control retinal bipolar cell specification or differentiation by repressing genes required for the development of other cell types...
  46. ncbi Transcriptional activation domain of the muscle-specific gene-regulatory protein myf5
    T Braun
    Department of Toxicology, Medical School, University of Hamburg, FRG
    Nature 346:663-5. 1990
    ..High-affinity sequence-specific DNA binding of myf5 also requires hetero-oligomeric association with the enhancer-binding protein E12 to confer muscle-specific transactivation...
  47. ncbi [Study on the characterization of the bi-directional promoter between pp38 gene and 1.8kb mRNA transcripts of Marek's disease viruses]
    Jia Bo Ding
    College of Animal Science, Shandong Agricultural University, Tai an 271018, China
    Wei Sheng Wu Xue Bao 45:363-7. 2005
    ..The results showed the activity of the divided promoters reduce on both directions, especially for the direction of 1.8kb mRNA transcript, nearly down to 1/41...
  48. ncbi GATA factors lie upstream of Nkx 2.5 in the transcriptional regulatory cascade that effects cardiogenesis
    Alison C Brewer
    Department of Cardiology, King s College Hospital, London SE5 9RS, UK
    Stem Cells Dev 14:425-39. 2005
    ....
  49. pmc Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb
    Matjaz Barboric
    Department of Medicine, Rosalind Russell Medical Research Center, University of California at San Francisco, San Francisco, CA 94143 0703, USA
    EMBO J 24:4291-303. 2005
    ..We propose a model where the interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct its binding to P-TEFb and subcellular localization that culminates in the inhibition of transcription...
  50. ncbi An analysis on transcriptional regulation activity of human XBP1 gene 5' upstream DNA sequences
    Feng Jin Guo
    Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, P R China
    Zhonghua Yi Xue Yi Chuan Xue Za Zhi 23:1-6. 2006
    ..To analyze the transcription activation and possible regulation mechanism of human X-box binding protein 1(XBP1)gene 5'upstream DNA sequence in different cell lines...
  51. ncbi Estradiol negatively regulates HIV-LTR promoter activity in glial cells
    Melinda E Wilson
    Department of Physiology, College of Medicine, University of Kentucky, Lexington, Kentucky 40536, USA
    AIDS Res Hum Retroviruses 22:350-6. 2006
    ..This study demonstrates a novel activity of 17beta-estradiol in glial cells that could play a role in the maintenance of neuronal health during HIV infection of the central nervous system...
  52. ncbi Positive and negative regulation of the transcription of the human protein kinase C beta gene
    Y S Niino
    Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan
    J Biol Chem 267:6158-63. 1992
    ..These results suggest the presence of multiple trans-acting factors that act on these positive and negative cis-acting elements and regulate the cell type-specific expression of the PKC beta gene...
  53. pmc Identification and cloning of a novel heterogeneous nuclear ribonucleoprotein C-like protein that functions as a transcriptional activator of the hepatitis B virus enhancer II
    N Tay
    W H O Collaborating Centre for Immunology, Faculty of Medicine, National University of Singapore
    J Virol 66:6841-8. 1992
    ..Cloned E2BP expressed in human fibroblasts by transient transfection displayed EII binding and activating characteristics similar to those of native E2BP in hepatocytes...
  54. ncbi Marek's disease virus unique genes pp38 and pp24 are essential for transactivating the bi-directional promoters for the 1.8 kb mRNA transcripts
    Jiabo Ding
    Animal Science and Technology College, Shandong Agricultural University, Taian, Shandong 271018, China
    Virus Genes 35:643-50. 2007
    ..By competitive inhibition tests with a set of DNA fragments from the promoter region, the sequence 5'-CTGCTCATTT-3' was identified as the core sequence for binding by pp38-pp24 in up-regulation of the bi-directional promoter activity...
  55. ncbi Analysis of promoter regions regulating basal and interleukin-4-inducible expression of the human CB1 receptor gene in T lymphocytes
    Christine Börner
    Department of Pharmacology and Toxicology, Magdeburg University, 44 Leipzigerstrasse, 39120 Magdeburg, Germany
    Mol Pharmacol 73:1013-9. 2008
    ..Taken together, these data show that CB1 receptors are expressed and up-regulated by interleukin-4 in T lymphocytes, which enables CB1-mediated communication to cells of other systems, such as neuronal cells...
  56. ncbi Transcriptional regulation of the human insulin receptor promoter
    K E Cameron
    Department of Medicine, University of California, San Diego, La Jolla 92093
    J Biol Chem 267:17375-83. 1992
    ....
  57. ncbi Complementarity, sequence and structural elements within the 3' and 5' non-coding regions of the Bunyamwera orthobunyavirus S segment determine promoter strength
    Alain Kohl
    Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK
    J Gen Virol 85:3269-78. 2004
    ....
  58. ncbi Myc-ARF (alternate reading frame) interaction inhibits the functions of Myc
    Abhishek Datta
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 900 S Ashland Avenue, Chicago, IL 60607, USA
    J Biol Chem 279:36698-707. 2004
    ..Our results strongly suggest that cMyc is a bona fide target of ARF and that ARF attenuates c-Myc independently of the ARF-p53 axis...
  59. ncbi Evaluation of combined gene regulatory elements for transcriptional targeting of suicide gene expression to malignant melanoma
    Heike Rothfels
    Skin Cancer Unit of the German Cancer Research Center DKFZ at the University Hospital Mannheim, Mannheim, Germany
    Exp Dermatol 12:799-810. 2003
    ..Our data indicate that the enhancer/tyrosinase and enhancer/MIA promoter constructs but not the viral promoter constructs can provide a valuable tool for selective suicide gene expression in melanoma...
  60. ncbi A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium
    T Okazaki
    Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan
    J Biol Chem 269:27855-62. 1994
    ..Thus, it is concluded that ref1 possesses transcription repressor activity in addition to its function as a transcriptional auxiliary protein...
  61. ncbi Regulation of alpha7 integrin expression during muscle differentiation
    Jianqiao Xiao
    Departments of Stomatology and Anatomy, University of California at San Francisco, San Francisco, California 94143 0422, USA
    J Biol Chem 278:49780-8. 2003
    ..Our results suggest that the interaction of c-Myc with this promoter region is an important regulatory element controlling alpha7 integrin expression during muscle development and myotendinous junction formation...
  62. ncbi HoxA10 represses gene transcription in undifferentiated myeloid cells by interaction with histone deacetylase 2
    YuFeng Lu
    Fineberg School of Medicine and Robert H Lurie Comprehensive Cancer Center, Northwestern University, and Chicago Lakeside Veterans Affairs Hospital, Illinois 60611, USA
    J Biol Chem 278:47792-802. 2003
    ..Our results suggest that insight into this problem can be obtained from biochemical characterization of the various molecular mechanisms of Hox protein function...
  63. ncbi The AF-1 and AF-2 domains of RAR gamma 2 and RXR alpha cooperate for triggering the transactivation and the degradation of RAR gamma 2/RXR alpha heterodimers
    Maurizio Gianni
    Institut de Génétique et de Biologie Moléculaire et Cellulaire IGBMC CNRS INSERM ULP, UMR 7104, BP 10142, 67404 Illkirch Cedex, France
    J Biol Chem 278:34458-66. 2003
    ..Our results underline that the AF-1 and AF-2 domains of each heterodimer partner cooperate with one other and that this cooperation is relevant for both the transcription and degradation processes...
  64. ncbi Regulation of mouse UBF gene by multiple growth-related control elements
    T Nishimura
    Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan
    Biochem Biophys Res Commun 205:1217-25. 1994
    ..Among serum response elements, members located between -1182 and -343 are indeed responsive to serum, suggesting their important role in the high expression of mUBF under cell growth conditions...
  65. pmc Analysis of fra-2 gene expression
    T Yoshida
    Department of Tumor Virus Research, University of Tokyo, Japan
    Nucleic Acids Res 21:2715-21. 1993
    ..Two typical AP-1 sequences are located between the major transcriptional initiation site and the coding sequence, and the binding activity of protein complexes to these sequences was induced by serum...
  66. ncbi Regulation by estrogen through the 5'-flanking region of the mouse calbindin-D28k gene
    R K Gill
    Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark 07103 2714, USA
    Mol Endocrinol 9:319-26. 1995
    ....
  67. ncbi Engineered long terminal repeats of retroviral vectors enhance transgene expression in hepatocytes in vitro and in vivo
    Kanji Yamaguchi
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 606 8507, Kyoto, Japan
    Mol Ther 8:796-803. 2003
    ..5- to 5-fold in comparison with a Moloney murine leukemia virus-based vector or a vector containing the wild-type LTR of SFFVp, respectively, in murine hepatocytes in vivo...
  68. ncbi Mutational analysis of the avian pneumovirus conserved transcriptional gene start sequence identifying critical residues
    Nicole L Edworthy
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    J Gen Virol 86:3343-7. 2005
    ..The L gene start sequence directed transcription at levels approximately 50 % below that of the consensus gene start sequence. These data suggest that there are common features in pneumovirus transcriptional control sequences...
  69. ncbi Identification of regulatory regions within the KAI1 promoter: a role for binding of AP1, AP2 and p53
    Alexandra Marreiros
    Oncology Research Centre, Level 2 Clinical Sciences Building, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, Australia
    Gene 302:155-64. 2003
    ..Our data suggested that a loss of KAI1 mRNA was not simply due to absence of AP2, AP1 or p53 expression...
  70. ncbi Auto-regulated hepatic insulin gene expression in type 1 diabetic rats
    R Chen
    Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Ther 3:584-90. 2001
    ..These results suggest that auto-regulated hepatic insulin expression can potentially be developed as an effective and safe treatment modality for type 1 diabetes...
  71. ncbi Role of IRS-3 in the insulin signaling of IRS-1-deficient brown adipocytes
    Monica Arribas
    Departamento de Bioquímica y Biología Molecular Instituto de Bioquímica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad Complutense, Facultad de Farmacia, Universidad Complutense, Madrid 28040, Spain
    J Biol Chem 278:45189-99. 2003
    ..Thus, our data further implicate a unique role for IRS-1 in triggering insulin action in brown adipocytes...
  72. ncbi Gain of oncogenic function of p53 mutants induces invasive phenotypes in human breast cancer cells by silencing CCN5/WISP-2
    Gopal Dhar
    Cancer Research Unit, VA Medical Center, Kansas City, Missouri, USA
    Cancer Res 68:4580-7. 2008
    ..Thus, these studies suggest that CCN5 inactivation could be an essential molecular event for p53 mutant-induced invasive phenotypes...
  73. ncbi Induction of IL-8 and monoclyte chemoattractant protein-1 by doxorubicin in human small cell lung carcinoma cells
    Misako Shibakura
    Department of Health Sciences, Okayama University Medical School, Japan
    Int J Cancer 103:380-6. 2003
    ....
  74. ncbi High-level expression of a foreign gene from the most 3'-proximal locus of a recombinant Newcastle disease virus
    Z Huang
    Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
    J Gen Virol 82:1729-36. 2001
    ..The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector...
  75. ncbi Transcriptionally active polymerase chain reaction (TAP): high throughput gene expression using genome sequence data
    Xiaowu Liang
    Gene Therapy Systems, Inc, San Diego, California 92121, USA
    J Biol Chem 277:3593-8. 2002
    ..This technology offers a practical approach to directly utilize genome sequence data to generate functional proteomes...
  76. ncbi Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum
    Samil Jung
    Research Center for Women s Diseases, Sookmyung Women s University, Seoul, Korea
    Can J Microbiol 56:178-87. 2010
    ..Taken together, we propose that a T-box mediated attenuation mechanism is responsible for the gene expression of the hisDCB-orf1-orf2-hisHA-impA-hisFI operon in C. glutamicum...
  77. ncbi beta-naphthoflavone, an inducer of xenobiotic metabolizing enzymes, inhibits firefly luciferase activity
    Thomas T Y Wang
    Phytonutrients Laboratory, ARS, BHNRC, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Anal Biochem 304:122-6. 2002
  78. ncbi Transcriptional regulation of fatty acid synthase gene by somatotropin in 3T3-F442A adipocytes
    D Yin
    Department of Dairy and Animal Science, The Pennsylvania State University, University Park 16802, USA
    J Anim Sci 79:2336-45. 2001
    ..These results suggest that a STRE may be located within the first 112 bp of the FAS promoter and that USF1 does not directly mediate the effect of ST on transcription of the FAS gene in 3T3-F442A adipocytes...
  79. ncbi The murine SP-C promoter directs type II cell-specific expression in transgenic mice
    Stephan W Glasser
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 288:L625-32. 2005
    ..8-kb SP-C promoter construct is highly dependent on its chromatin environment...
  80. pmc Insulin and dexamethasone induce GLUT4 gene expression in foetal brown adipocytes: synergistic effect through CCAAT/enhancer-binding protein alpha
    Rosario Hernandez
    Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    Biochem J 372:617-24. 2003
    ..Our results show that the synergism between insulin and glucocorticoids on glucose uptake is a consequence of the activation of the GLUT4 promoter by the transcription factor C/EBPalpha...
  81. ncbi The nectary-specific pattern of expression of the tobacco Nectarin I promoter is regulated by multiple promoter elements
    Clay Carter
    Department of Biochemistry Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Plant Mol Biol 51:451-7. 2003
    ..Analysis in transgenic plants revealed that the nectary-specific expression is the result of multiple promoter elements and suggests that nectar secretion and flower opening may be coordinately regulated...
  82. ncbi Glucocorticoid inhibits the human pro-interleukin lbeta gene (ILIB) by decreasing DNA binding of transactivators to the signal-responsive enhancer
    Wayne R Waterman
    Philadelphia College of Osteopathic Medicine, Department of Neurosurgery, Philadelphia, PA 19131, USA
    Mol Immunol 43:773-82. 2006
    ..Thus, GR likely disrupts the DNA binding capability of critical IL1B factors via transrepression...
  83. ncbi Effects of DNA vaccine in murine malaria using a full-length cDNA library
    A Shibui
    Department of Virology, Institute of Medical Science, The University of Tokyo, Japan
    Res Commun Mol Pathol Pharmacol 109:147-57. 2001
    ..These observations suggested the possibility that the vaccination had an adverse effect on the cellular immunity that resulted in the development of severe malaria in BALB/c mice, which do not usually develop cerebral malaria...
  84. ncbi Cell-specific and developmental regulation of rabbit surfactant protein B promoter in transgenic mice
    C C Adams
    Department of Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708-3154, USA
    Am J Physiol Lung Cell Mol Physiol 280:L724-31. 2001
    ..Additionally, these data suggest that the -2,176/-730- and -730/-236-bp regions contain the DNA elements that repress and enhance SP-B gene transcription, respectively...
  85. ncbi Mouse 230-kDa bullous pemphigoid antigen gene: structural and functional characterization of the 5'-flanking region and interspecies conservation of the deduced amino-terminal peptide sequence of the protein
    D Sawamura
    Department of Dermatology, Hirosaki University School of Medicine, Japan
    J Invest Dermatol 103:651-5. 1994
    ..Furthermore, these experiments suggested that the upstream sequences contain upregulatory elements, as well as elements that confer, at least in part, tissue specificity to the expression of the mouse 230-kDa BPA gene...
  86. ncbi Tissue and developmental specific expression of murine smooth muscle gamma-actin fusion genes in transgenic mice
    J Qian
    Division of Developmental Biology, Children s Hospital Medical Center, College of Medicine, University of Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 207:135-44. 1996
    ..Overall, the studies suggest that upstream elements between -2.7 kb and -571 bp and elements within intron 1 are required for high levels of SMGA gene expression in an appropriate temporal-spatial fashion...
  87. pmc trans activation of an immediate-early frog virus 3 promoter by a virion protein
    D B Willis
    J Virol 56:495-501. 1985
    ..The results suggest that a virion protein alters either the DNA template or the host polymerase to allow transcription from immediate-early FV3 promoters...
  88. ncbi Inhibition of thyroid hormone action by a non-hormone binding c-erbA protein generated by alternative mRNA splicing
    R J Koenig
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115
    Nature 337:659-61. 1989
    ..Alternative splicing of the erbA alpha transcript thus generates products with opposing biological activities, suggesting a novel mechanism for the modulation of hormonal responsiveness...
  89. ncbi Characterization of hypersensitive sites, protein-binding motifs, and regulatory elements in both promoters of the mouse porphobilinogen deaminase gene
    C Porcher
    Laboratoire de Genetique Moleculaire, Faculte X Bichat, Paris, France
    J Biol Chem 266:10562-9. 1991
    ....
  90. ncbi Isolation and characterization of the platelet-derived growth factor beta receptor promoter
    E Shinbrot
    Daiichi Research Center, Cardiovascular Research Institute, University of California, San Francisco 94143 0130, USA
    Dev Dyn 208:211-9. 1997
    ..The further analysis of this promoter will help elucidate the transcriptional regulation of expression of the PDGFbeta r, and provide a useful tool for directing expression of heterologous genes...
  91. ncbi Structure of the entire human muscle phosphofructokinase-encoding gene: a two-promoter system
    T Yamasaki
    Second Department of Internal Medicine, Osaka University Medical School, Japan
    Gene 104:277-82. 1991
    ..These results indicated that the hPFK-M gene contains at least two promoter regions, facilitating the expression of the heterogeneous gene transcripts in a cell-type-specific manner...
  92. ncbi Induction of tyrosine hydroxylase protein and a transgene containing tyrosine hydroxylase 5' flanking sequences by stress in mouse adrenal gland
    C A Osterhout
    Department of Pharmacology and Physiology, University of Rochester Medical Center, New York 14642, USA
    J Neurochem 68:1071-7. 1997
    ..Our results indicate that these transgenic mice are an excellent model system to study the molecular mechanisms regulating TH gene expression in adrenal medulla...
  93. pmc Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter
    M I Chung-Bok
    Department of Nutritional Sciences, Thompson Hall, Cook College, Rutgers, The State University of New Jersey, New Brunswick, NJ 08903, USA
    Biochem J 324:193-200. 1997
    ..The region responsive to glucocorticoids was mapped to -252 to -103 relative to the transcription start site...
  94. ncbi Structural and functional analysis of the promoter of the murine V gamma 1.1 T cell receptor gene
    R Ofir
    Department of Microbiology and Immunology, Ben Gurion University of the Negev, Beer Sheva, Israel
    Eur J Immunol 25:3070-8. 1995
    ..The possible function of the Sp1-like protein in silencing the minimal promoter activity is discussed...
  95. ncbi Differences in inducibility of CYP1A1-mRNA by benzimidazole compounds between human and mouse cells: evidences of a human-specific signal transduction pathway for CYP1A1 induction
    H Kikuchi
    Institute of Development, Aging, and Cancer, Division of Gene Research, Tohoku University, Sendai, Japan
    Arch Biochem Biophys 334:235-40. 1996
    ..It is thus concluded that metabolites of OP in human cells are not the ligands for the human Ah receptor. Therefore, in human cells, but not mouse cells, there must be an OP-sensitive activation factor for the human Ah receptor...
  96. ncbi A critical role of Sp1- and Ets-related transcription factors in maintaining CTL-specific expression of the mouse perforin gene
    B S Youn
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Immunol 157:3499-509. 1996
    ..5- to 3-fold reduction of CAT activity. The results suggest that a combination of the three proximal cis-acting elements may constitute a minimal region responsible for CTL-specific expression of perforin...
  97. ncbi The POZ domain: a conserved protein-protein interaction motif
    V J Bardwell
    Imperial Cancer Research Fund Laboratories, London, UK
    Genes Dev 8:1664-77. 1994
    ..We discuss the implications of multimerization for the function of POZ domain proteins...
  98. pmc Cloning and characterization of the human osteopontin gene and its promoter
    N Hijiya
    Department of Pathology, Oita Medical University, Japan
    Biochem J 303:255-62. 1994
    ....
  99. pmc Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis
    S J Li
    Department of Microbiology, University of Illinois, Urbana Champaign 61801
    J Bacteriol 175:332-40. 1993
    ..For example, the accA promoter was localized within the upstream polC gene (which encodes the DNA polymerase III catalytic subunit), suggesting that additional regulatory mechanisms exist...
  100. ncbi Molecular characterization of a developmentally regulated porcine skeletal myosin heavy chain gene and its 5' regulatory region
    K C Chang
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, UK
    J Cell Sci 108:1779-89. 1995
    ..Interestingly, for high levels of CAT expression to occur, a 3' region, extending from the transcriptional start site to part. of intron 2, must be present in all the DNA constructs used...
  101. ncbi Multiple functions of the TR2-11 orphan receptor in modulating activation of two key cis-acting elements involved in the retinoic acid signal transduction system
    T M Lin
    University of Wisconsin Comprehensive Cancer Center, University of Wisconsin, Madison 53792, USA
    J Biol Chem 270:30121-8. 1995
    ..Together, our data suggest that the TR2 orphan receptor may be a master regulator in modulating the activation of two key HREs, RARE beta and CRBPIIp, involved in the retinoic acid signal transduction pathway...

Research Grants49

  1. Development of an VEE Replicon Vaccine Against Smallpox
    Kurt Kamrud; Fiscal Year: 2005
    ....
  2. THE ROLE OF YEAST MITOCHONDRIA AND CANCER
    JULIUS MARMUR; Fiscal Year: 1980
    ..The structural mutants will be used in transformation experiments to clone the wild type alleles. ..
  3. Mechanisms of Growth Factor-Estrogen Receptor Crosstalk
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
  4. Functional aspects of SREBP1c in intact skeletal muscle
    Paul MacLean; Fiscal Year: 2007
    ....
  5. TSK-2: A NEW ANIMAL MODEL FOR SCLERODERMA
    Paul Christner; Fiscal Year: 2004
    ..abstract_text> ..
  6. GENE REGULATION AND FUNCTION OF AMELOBLASTIN
    Paul Krebsbach; Fiscal Year: 2003
    ....
  7. InteractiveSlides - Microarray data visualization tool
    Jozef Spychala; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  8. Neurotrophins and Tyrosine Kinases in Prostate Cancer
    Christopher Evans; Fiscal Year: 2006
    ..This proposal is based on significant preliminary data and has strong translational potential for future proposals. ..
  9. GENETICS OF RECURRENT EARLY-ONSET DEPRESSION (GENRED)
    GEORGE ZUBENKO; Fiscal Year: 2002
    ..A timetable is proposed for rapid sharing of all biological materials, blinded clinical data, genotypes and linkage analyses with the scientific community through the NIMH-sponsored Center for Genetic Studies. ..
  10. PERMISSIVE RETINAL GENE EXPRESSION VIA HYPOMETHYLATION
    JEFFREY BOATRIGHT; Fiscal Year: 2001
    ..abstract_text> ..
  11. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2009
    ..An understanding of the inability of 158-1 to resist host defenses may offer an unique opportunity to explore interactions between the mycoplasma and innate immunity. ..
  12. Colon Cancer Inhibition by a Class of PPARgamma Agonists
    Stephen Safe; Fiscal Year: 2009
    ..The proposed studies will provide mechanistic insights on PPARy-dependent inhibition of colon cancer and identify compounds for future clinical studies. ..
  13. Coactivation of Estrogen Receptor alpha/Sp1
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
  14. Chaperones, chromatin, and transcriptional control by PR
    STEVEN NORDEEN; Fiscal Year: 2006
    ..Together, the studies proposed will provide fundamental knowledge that will advance our understanding of steroid receptor action in human health and disease. ..
  15. MOLECULAR REGULATION OF ARTERIAL THROMBOSIS
    Robert Simari; Fiscal Year: 2009
    ..Specific Aim 2: To define the role of endothelial-derived TFPI on vascular thrombosis. Specific Aim 3: To define the role of endothelial-derived TFPI on vascular remodeling including angiogenesis. ..
  16. HIV protease inhibitors, marcophage function/estrogen
    Melinda Wilson; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  17. GENETIC DISSECTION OF TOXOPLASMA GONDII
    David Roos; Fiscal Year: 2008
    ..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
  18. EXERCISE HYPERTROPHY AND CONTROL OF MYOSIN INDUCTION
    Richard Tsika; Fiscal Year: 2008
    ..abstract_text> ..
  19. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
  20. ALTERED MECHANICAL LOADS AND SKELETAL MUSCLE PHENOTYPE
    Richard Tsika; Fiscal Year: 2009
    ..abstract_text> ..
  21. TOXICOLOGY OF ENVIRONMENT CONTAMINANTS
    Stephen Safe; Fiscal Year: 2007
    ..The proposed training program will continue to provide trainees with a challenging academic and research environment that will prepare them for future careers in academic, industry and government. ..
  22. Enhancement of the glycosylation machinery of the hen bioreactor.
    ALEX HARVEY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. MORPHOLOGIC/NEUROCHEMICAL CORRELATES OF DEPRESSION IN AD
    GEORGE ZUBENKO; Fiscal Year: 2009
    ..abstract_text> ..
  24. Estrogen and progestin crosstalk via binding of PR at estrogen response elements
    STEVEN NORDEEN; Fiscal Year: 2008
    ..abstract_text> ..
  25. Postsynthetic Modifications of Bacterial Membrane Lipids
    John Cronan; Fiscal Year: 2006
    ..These processes will be studied by a combination of biochemical, genetic, and molecular biological approaches including tests of pathogenesis in a mouse model system ..
  26. 2,3,7,8,--TCDD Effects on the Estrogenic Responses
    Stephen Safe; Fiscal Year: 2006
    ..The proposed Aims will address important mechanistic considerations on IDRE-independent inhibitory AhR-ERa crosstalk and pathways critical for inhibition of mammary tumor growth by SAhRMs. ..
  27. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
  28. GENE REGULATION AND FUNCTION OF AMELOBLASTIN
    Paul Krebsbach; Fiscal Year: 2002
    ....
  29. Genetics of Sagg: A Heritable Mouse Model for Cutis Laxa
    Paul Christner; Fiscal Year: 2004
    ..These experiments will also include genes encoding enzymes involved in the degradation and removal of the EMC. These studies will be undertaken in order to elucidate the downstream molecular events resulting from the Sagg mutation. ..
  30. ESTROGEN INDUCED RESPONSES VIA ER/SPL COMPLEXES
    Stephen Safe; Fiscal Year: 2002
    ..These results will ultimately facilitate design of chemotherapeutic approaches which target specific genes. ..
  31. Vasoprotective Actions of Autologous Cell Transfer
    Robert Simari; Fiscal Year: 2007
    ..Specific Aim 4: To optimize systemic transgene expression from genetically modified EOCs and to determine their systemic vasoprotective potential. [unreadable] [unreadable]..
  32. ECM in Structure & Function of the Craniofacial Complex
    Paul Krebsbach; Fiscal Year: 2005
    ..The program will focus on the following areas: The Role of the Extracellular Matrix in: A) Suture formation B) Bone C) Teeth: Enamel D) Dentin and Cementum E) Temporomandibular Joint. ..
  33. ANTIGENIC VARIATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ....
  34. Inhibition of Pancreatic Cancer by New PPARgamma Agonis*
    Stephen Safe; Fiscal Year: 2008
    ..PPAR? -active C-substituted DIMs that inhibit pancreatic cancer cell growth in vitro and in vivo, and these will serve as lead compounds for development of new drugs for clinical treatment of this devastating disease ..
  35. Estrogen and HIV dementia
    Melinda Wilson; Fiscal Year: 2003
    ..abstract_text> ..
  36. BONE MARROW STROMAL CELLS FOR SKELETAL REGENERATION
    Paul Krebsbach; Fiscal Year: 2005
    ..The information gained from this study will provide an essential prerequisite for the development of regenerative approaches that utilize bone marrow stromal cells for gene-and cell-based therapies. ..
  37. NASCENT PEPTIDE CONTROL OF THE RIBOSOME
    PAUL LOVETT; Fiscal Year: 2001
    ..We have identified a simple method that should allow determination of the critical amino acids for binding. Further, we will refine the identification of the rRNA site for peptide binding by high resolution footprinting. ..
  38. Ninth International Conference on the Chemistry and Biology of Mineralized Tissue
    Paul Krebsbach; Fiscal Year: 2007
    ..The ensuing "cross pollenization" of perspective and expertise is the ideal venue to enhance the process of discovery in this important field. [unreadable] [unreadable] [unreadable]..
  39. ALKYL PCDFS--INHIBITION OF MAMMARY CANCER
    Stephen Safe; Fiscal Year: 2001
    ..Thus the proposed studies will define a new mechanism-based class of antiestrogens that can be used alone or in combination with tamoxifen for treatment of breast and endometrial cancer. ..
  40. HIV as a key regulator of c-MYB in human cells
    Robert Ramsay; Fiscal Year: 2004
    ..We hypothesize that Tat activates c-MYB in macrophages and that a thorough understanding of this process will advance our goal of blocking HIV transcription. ..
  41. Avian transgenesis via site-directed integration
    ALEX HARVEY; Fiscal Year: 2006
    ..This novel technology will expedite the creation of chickens that can produce Pharmaceuticals, thereby facilitating drug development, enhancing clinical trials and lowering the cost of Pharmaceuticals. ..
  42. MOLECULAR MECHANISMS OF GLUCOCORTICOID HORMONE ACTION
    STEVEN NORDEEN; Fiscal Year: 2004
    ..Together these studies will make significant contributions to our understanding of hormone action. ..
  43. Materials/Biologic Constructs for Skeletal Regeneration
    Paul Krebsbach; Fiscal Year: 2002
    ..Bone and cartilage formation in our anatomic-specific designed bioengineered materials will facilitate innovative strategies for the reconstruction of human joints. ..
  44. BIOLOGICAL MARKER FOR PRIMARY DEMENTIA IN THE ELDERLY
    GEORGE ZUBENKO; Fiscal Year: 2003
    ....
  45. GENETIC ANALYSIS OF GBS ADHERENCE TO FIBRONECTIN
    GLEN TAMURA; Fiscal Year: 2002
    ..These studies will allow for identification of the structural gene(s) for fibronectin adhesin(s). ..
  46. CRANIOFACIAL SKELETAL TISSUE ENGINEERING CONFERENCE
    Sunil Kapila; Fiscal Year: 2008
    ..The meeting will be limited to 250 participants who have a background in bioengineering. ..
  47. Craniofacial Imaging in the 21st Century
    Sunil Kapila; Fiscal Year: 2002
    ..The meeting will be limited to 300 participants who have a background in imaging. This symposia series will provide momentum to discoveries that will be important to craniofacial sciences and patient care. ..
  48. Fibronectin Fragment-Induced Osteolysis Mediated by MMPs
    Sunil Kapila; Fiscal Year: 2009
    ..These studies will provide a fundamental understanding of the basis for bone loss in periodontal disease and other osteolytic diseases. ..
  49. In Vivo Antiviral Activity of Saponins
    MICHAEL RONER; Fiscal Year: 2007
    ..The extract is well suited to such an application as it is pH stable, is already approved as a food additive, is inexpensive, readily available and is soluble in water. [unreadable] [unreadable] [unreadable]..