acyltransferases

Summary

Summary: Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.

Top Publications

  1. pmc Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
    A Dahlqvist
    Scandinavian Biotechnology Research ScanBi AB, Herman Ehles Väg 2 S 26831 Svalôv, Sweden
    Proc Natl Acad Sci U S A 97:6487-92. 2000
  2. pmc A fluorescence-based assay for N-myristoyltransferase activity
    Victor Goncalves
    Department of Chemistry, Imperial College London, London SW7 2AZ, UK
    Anal Biochem 421:342-4. 2012
  3. pmc Ghrelin octanoylation mediated by an orphan lipid transferase
    Jesus A Gutierrez
    Integrative Biology, Eli Lilly and Company, 2001 West Main Street, Greenfield, IN 46140, USA
    Proc Natl Acad Sci U S A 105:6320-5. 2008
  4. pmc Global analysis of protein palmitoylation in yeast
    Amy F Roth
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell 125:1003-13. 2006
  5. ncbi DHHC palmitoyl transferases: substrate interactions and (patho)physiology
    Jennifer Greaves
    Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
    Trends Biochem Sci 36:245-53. 2011
  6. pmc N-myristoyltransferase inhibitors as new leads to treat sleeping sickness
    Julie A Frearson
    Drug Discovery Unit, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee DD1 5EH, UK
    Nature 464:728-32. 2010
  7. ncbi Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality
    Gail Shadle
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Phytochemistry 68:1521-9. 2007
  8. ncbi Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function
    Roshni R Singaraja
    950 West 28th Ave, Vancouver, BC, V5Z 4H4, Canada
    Circ Res 105:138-47. 2009
  9. pmc Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas
    Nanette R Boyle
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 287:15811-25. 2012
  10. ncbi Storage lipid synthesis is non-essential in yeast
    Line Sandager
    Department of Crop Science, Swedish University of Agricultural Sciences, Box 44, S 230 53 Alnarp, Sweden
    J Biol Chem 277:6478-82. 2002

Detail Information

Publications273 found, 100 shown here

  1. pmc Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
    A Dahlqvist
    Scandinavian Biotechnology Research ScanBi AB, Herman Ehles Väg 2 S 26831 Svalôv, Sweden
    Proc Natl Acad Sci U S A 97:6487-92. 2000
    ..However, budding yeast PDAT and its relatives in fission yeast and Arabidopsis form a distinct branch within this protein superfamily, indicating that a separate PDAT enzyme arose at an early point in evolution...
  2. pmc A fluorescence-based assay for N-myristoyltransferase activity
    Victor Goncalves
    Department of Chemistry, Imperial College London, London SW7 2AZ, UK
    Anal Biochem 421:342-4. 2012
    ..We also describe expression and characterization of NMT1 and NMT2 and assay validation with small molecule inhibitors. This assay should be broadly applicable to NMTs from a range of organisms...
  3. pmc Ghrelin octanoylation mediated by an orphan lipid transferase
    Jesus A Gutierrez
    Integrative Biology, Eli Lilly and Company, 2001 West Main Street, Greenfield, IN 46140, USA
    Proc Natl Acad Sci U S A 105:6320-5. 2008
    ..The occurrence of ghrelin and GOAT in stomach and pancreas tissues demonstrates the relevance of GOAT in the acylation of ghrelin and further implicates acylated ghrelin in pancreatic function...
  4. pmc Global analysis of protein palmitoylation in yeast
    Amy F Roth
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell 125:1003-13. 2006
    ..e., protein acyltransferases (PATs)--were identified only recently...
  5. ncbi DHHC palmitoyl transferases: substrate interactions and (patho)physiology
    Jennifer Greaves
    Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
    Trends Biochem Sci 36:245-53. 2011
    ..Recent studies have highlighted how DHHC proteins regulate cell function and influence physiology and pathophysiology...
  6. pmc N-myristoyltransferase inhibitors as new leads to treat sleeping sickness
    Julie A Frearson
    Drug Discovery Unit, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee DD1 5EH, UK
    Nature 464:728-32. 2010
    ..Our studies validate T. brucei N-myristoyltransferase as a promising therapeutic target for human African trypanosomiasis...
  7. ncbi Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality
    Gail Shadle
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Phytochemistry 68:1521-9. 2007
    ..Although manipulation of lignin biosynthesis can greatly improve forage digestibility, accompanying effects on plant development need to be better understood...
  8. ncbi Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function
    Roshni R Singaraja
    950 West 28th Ave, Vancouver, BC, V5Z 4H4, Canada
    Circ Res 105:138-47. 2009
    ..Thus, palmitoylation regulates ABCA1 localization at the plasma membrane, and regulates its lipid efflux ability...
  9. pmc Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas
    Nanette R Boyle
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 287:15811-25. 2012
    ..Three genes encoding acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG ..
  10. ncbi Storage lipid synthesis is non-essential in yeast
    Line Sandager
    Department of Crop Science, Swedish University of Agricultural Sciences, Box 44, S 230 53 Alnarp, Sweden
    J Biol Chem 277:6478-82. 2002
    ..The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies. We conclude that neither storage lipids nor lipid bodies are essential for growth in yeast...
  11. pmc Mechanism of endophilin N-BAR domain-mediated membrane curvature
    Jennifer L Gallop
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 25:2898-910. 2006
    ..Its presence defines an endophilin/nadrin subclass of BAR domains. We propose that N-BAR domains function as low-affinity dimers regulating binding partner recruitment to areas of high membrane curvature...
  12. ncbi Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis
    J T Belisle
    Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
    Science 276:1420-2. 1997
    ..Furthermore, the use of an antagonist (6-azido-6-deoxy-alpha, alpha'-trehalose) of this activity demonstrates that these proteins are essential and potential targets for new antimycobacterial drugs...
  13. ncbi Ag85B-ESAT-6 adjuvanted with IC31 promotes strong and long-lived Mycobacterium tuberculosis specific T cell responses in naïve human volunteers
    Jaap T van Dissel
    Leiden University Medical Center, Department of Infectious Diseases, Leiden, The Netherlands
    Vaccine 28:3571-81. 2010
    ..These strong responses persisted through 2.5 years of follow-up, indicating the induction of a substantial memory response in the vaccine recipients...
  14. pmc Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis
    S Cases
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 95:13018-23. 1998
    ..The identification of this cDNA encoding a DGAT will greatly facilitate studies of cellular glycerolipid metabolism and its regulation...
  15. pmc GOAT links dietary lipids with the endocrine control of energy balance
    Henriette Kirchner
    Departments of Psychiatry and Medicine, Obesity Research Centre and Genome Research Institute, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    Nat Med 15:741-5. 2009
    ..These data implicate the ghrelin-GOAT system as a signaling pathway that alerts the central nervous system to the presence of dietary calories, rather than to their absence as is commonly accepted...
  16. pmc Glucose and weight control in mice with a designed ghrelin O-acyltransferase inhibitor
    Brad P Barnett
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 330:1689-92. 2010
    ..In addition to serving as a research tool for mapping ghrelin actions, GO-CoA-Tat may help pave the way for clinical targeting of GOAT in metabolic diseases...
  17. ncbi A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1
    Rainer Kalscheuer
    Institut fur Mikrobiologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, Germany
    J Biol Chem 278:8075-82. 2003
    ..calcoaceticus. This novel type of long-chain acyl-CoA acyltransferase is not related to known acyltransferases including the WS from jojoba (Simmondsia chinensis), the DGAT1 or DGAT2 families present in yeast, plants, ..
  18. pmc Discovery of Plasmodium vivax N-myristoyltransferase inhibitors: screening, synthesis, and structural characterization of their binding mode
    Victor Goncalves
    Department of Chemistry, Imperial College London, London SW7 2AZ, United Kingdom
    J Med Chem 55:3578-82. 2012
    ..A set of analogues was designed and tested to define the chemical groups relevant for activity and selectivity...
  19. pmc Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis
    Laurent Hoffmann
    Institut de Biologie Moleculaire des Plantes, Unite Propre de Recherche, 2357 du Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67000 Strasbourg, France
    Plant Cell 16:1446-65. 2004
    ..These results demonstrate the function of the acyltransferase in phenylpropanoid biosynthesis...
  20. ncbi Enhanced immunogenicity and protective efficacy against Mycobacterium tuberculosis of bacille Calmette-Guérin vaccine using mucosal administration and boosting with a recombinant modified vaccinia virus Ankara
    Nilu P Goonetilleke
    Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 171:1602-9. 2003
    ..These findings support further evaluation of mucosally targeted prime-boost vaccination approaches for tuberculosis...
  21. ncbi Acyltransferases in plants: a good time to be BAHD
    John C D'Auria
    Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, D 07745 Jena, Germany
    Curr Opin Plant Biol 9:331-40. 2006
    Acylation is a common and biochemically significant modification of plant secondary metabolites. Plant BAHD acyltransferases constitute a large family of acyl CoA-utilizing enzymes whose products include small volatile esters, modified ..
  22. pmc Neutral lipid biosynthesis in engineered Escherichia coli: jojoba oil-like wax esters and fatty acid butyl esters
    Rainer Kalscheuer
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat, Corrensstrasse 3, D 48149 Munster, Germany
    Appl Environ Microbiol 72:1373-9. 2006
    ..coli, which might open new perspectives for the biotechnological production of cheap jojoba oil equivalents from inexpensive resources employing recombinant microorganisms...
  23. ncbi Palmitoylation of intracellular signaling proteins: regulation and function
    Jessica E Smotrys
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Annu Rev Biochem 73:559-87. 2004
    ..The regulation of palmitoylation occurs through the actions of protein acyltransferases and protein acylthioesterases...
  24. ncbi Protein palmitoylation by a family of DHHC protein S-acyltransferases
    David A Mitchell
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA
    J Lipid Res 47:1118-27. 2006
    ..This review presents the genetic and biochemical evidence for the PAT activity of DHHC proteins and discusses the mechanism of protein-mediated palmitoylation...
  25. pmc Ghrelin O-acyltransferase (GOAT) is essential for growth hormone-mediated survival of calorie-restricted mice
    Tong Jin Zhao
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 107:7467-72. 2010
    ..Thus, an essential function of ghrelin in mice is elevation of GH levels during severe calorie restriction, thereby preserving blood glucose and preventing death...
  26. pmc Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture
    Jaiyanth Daniel
    University of Central Florida, Biomolecular Science Center, BMS 136, 4000 Central Florida Blvd, Orlando, FL 32816 2364, USA
    J Bacteriol 186:5017-30. 2004
    ..Therefore, TG synthesis may be an appropriate target for novel antilatency drugs that can prevent the organism from surviving dormancy and thus assist in the control of tuberculosis...
  27. ncbi Phytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism
    Philippe Jeandet
    Laboratoire d Oenologie, URVVC, UPRES EA 2069, Faculte des Sciences, Universite de Reims, B P 1039, 51687 Reims Cedex 02, France
    J Agric Food Chem 50:2731-41. 2002
    ....
  28. ncbi Single mucosal, but not parenteral, immunization with recombinant adenoviral-based vaccine provides potent protection from pulmonary tuberculosis
    Jun Wang
    Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Centre for Gene Therapeutics, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
    J Immunol 173:6357-65. 2004
    ..Our study also lends strong evidence that respiratory mucosal vaccination is critically advantageous over systemic routes of vaccination against TB...
  29. pmc N-myristoyltransferase from Leishmania donovani: structural and functional characterisation of a potential drug target for visceral leishmaniasis
    James A Brannigan
    York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5YW, UK
    J Mol Biol 396:985-99. 2010
    ..These studies validate L. donovani NMT as a potential target for development of new therapeutic agents against visceral leishmaniasis...
  30. pmc DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development
    Meng Zhang
    Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Cell 21:3885-901. 2009
    ....
  31. ncbi Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteins
    Yusuke Ohno
    Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 jo, Nishi 6 choume, Kita ku, Sapporo 060 0812, Japan
    Biochim Biophys Acta 1761:474-83. 2006
    Increasing evidence indicates that DHHC cysteine-rich domain-containing proteins (DHHC proteins) are protein acyltransferases. Although multiple DHHC proteins are found in eukaryotes, characterization has been examined for only a few...
  32. ncbi A superfamily of membrane-bound O-acyltransferases with implications for wnt signaling
    K Hofmann
    Bioinformatics and Gene Discovery Group, MEMOREC Stoffel GmbH, Stöckheimer Weg 1, D 50829 Köln, Germany
    Trends Biochem Sci 25:111-2. 2000
  33. ncbi Poly(3-hydroxybutyrate) granule-associated proteins: impacts on poly(3-hydroxybutyrate) synthesis and degradation
    Markus Pötter
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, 48149 Munster, Germany
    Biomacromolecules 6:552-60. 2005
    ..In addition, putative applications of PHA granules and PHA granule-associated proteins in nanotechnology are discussed...
  34. pmc Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic arabidopsis
    K D Lardizabal
    Calgene Campus, Monsanto, 1920 Fifth Street, Davis, California 95616, USA
    Plant Physiol 122:645-55. 2000
    ..Gas chromatography analysis of transmethylated oil from individual seeds suggested that wax levels may represent up to 70% (by weight) of the oil present in those seeds...
  35. ncbi Dynamic protein palmitoylation in cellular signaling
    Tsuyoshi Iwanaga
    Division of Membrane Physiology, Department of Cell Physiology, National Institute for Physiological Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
    Prog Lipid Res 48:117-27. 2009
    ..Integrated approaches including advanced proteomics, live-cell imaging, and molecular genetics are beginning to clarify the molecular machinery for palmitoylation reaction in diverse aspects of cellular functions...
  36. ncbi AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34
    Anil K Agarwal
    Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390, USA
    Nat Genet 31:21-3. 2002
    ..AGPAT2 mRNA is highly expressed in adipose tissue. We conclude that mutations in AGPAT2 may cause congenital generalized lipodystrophy by inhibiting triacylglycerol synthesis and storage in adipocytes...
  37. ncbi A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast
    P Oelkers
    Institute of Human Nutrition, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 275:15609-12. 2000
    ..Saccharomyces cerevisiae, we investigated five candidate genes with sequence conservation to mammalian acyltransferases. Four of these genes are similar to the recently identified acyl-CoA diacylglycerol acyltransferase and, when ..
  38. pmc Multifunctional, high-level cytokine-producing Th1 cells in the lung, but not spleen, correlate with protection against Mycobacterium tuberculosis aerosol challenge in mice
    Emily K Forbes
    The Jenner Institute, University of Oxford, Headington, Oxford, United Kingdom
    J Immunol 181:4955-64. 2008
    ..Successful immunization regimes appear to induce Ag-specific cells with abundant intracellular cytokine staining...
  39. ncbi Recent advances in the biosynthesis and accumulation of anthocyanins
    Karin Springob
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Nat Prod Rep 20:288-303. 2003
    ..The literature from 1997 to the beginning of 2002 is reviewed, and 163 references are cited...
  40. ncbi Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase
    P Bouvier-Nave
    Institut de Biologie Moleculaire des Plantes, Strasbourg, France
    Eur J Biochem 267:85-96. 2000
    During the course of a search for cDNAs encoding plant sterol acyltransferases, an expressed sequence tag clone presenting substantial identity with yeast and animal acyl CoA:cholesterol acyltransferases was used to screen cDNA libraries ..
  41. pmc Cardiolipin remodeling by ALCAT1 links oxidative stress and mitochondrial dysfunction to obesity
    Jia Li
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Cell Metab 12:154-65. 2010
    ..Collectively, these findings identify a key role of ALCAT1 in regulating CL remodeling, mitochondrial dysfunction, and susceptibility to DIO...
  42. ncbi Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism
    Laurent Hoffmann
    Institut de Biologie Moleculaire des Plantes, CNRS, Universite Louis Pasteur, 12, rue du Général Zimmer, 67000 Strasbourg, France
    J Biol Chem 278:95-103. 2003
    ..Thus, hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase appears to control the biosynthesis and turnover of major plant phenolic compounds such as lignin and chlorogenic acid...
  43. pmc Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family
    Jeffrey B Cheng
    Department of Molecular Genetics, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 279:37798-807. 2004
    ..little or no ability to synthesize cholesteryl esters, diacylglycerols, or triacylglycerols, whereas other acyltransferases, including the acyl-CoA:monoacylglycerol acyltransferase 1 and 2 enzymes and the acyl-CoA:diacylglycerol ..
  44. pmc Deficiency of the intestinal enzyme acyl CoA:monoacylglycerol acyltransferase-2 protects mice from metabolic disorders induced by high-fat feeding
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA
    Nat Med 15:442-6. 2009
    ....
  45. ncbi Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurate
    T Clementz
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:12095-102. 1996
    ..Transfer of palmitate, palmitoleate, or R-3-hydroxymyristate was very slow. Taken together with previous studies, our findings indicate that htrB encodes a key, late functioning acyltransferase of lipid A biosynthesis...
  46. ncbi Human CD8+ CTL specific for the mycobacterial major secreted antigen 85A
    S M Smith
    Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom
    J Immunol 165:7088-95. 2000
    ..These results demonstrate a previously unrecognized, MHC class I-restricted, CD8(+) CTL response to a major secreted Ag of mycobacteria and supports the use of Ag85A as a candidate vaccine against tuberculosis...
  47. pmc Malleability of the folding mechanism of the outer membrane protein PagP: parallel pathways and the effect of membrane elasticity
    Gerard H M Huysmans
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    J Mol Biol 416:453-64. 2012
    ..The results highlight the effect of lipid composition in tailoring the folding mechanism of a membrane protein, revealing that membrane proteins have access to multiple, competing folding routes to a unique native structure...
  48. pmc Lecithin-retinol acyltransferase is essential for accumulation of all-trans-retinyl esters in the eye and in the liver
    Matthew L Batten
    Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:10422-32. 2004
    ..We conclude that Lrat-/- mice may serve as an animal model with early onset severe retinal dystrophy and severe retinyl ester deprivation...
  49. pmc Selective inhibitors of protozoan protein N-myristoyltransferases as starting points for tropical disease medicinal chemistry programs
    Andrew S Bell
    Worldwide Medicinal Chemistry, Pfizer Worldwide Research and Development, Kent, UK
    PLoS Negl Trop Dis 6:e1625. 2012
    ....
  50. ncbi The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene
    J Zou
    National Research Council of Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada
    Plant J 19:645-53. 1999
    ..which is similar to a recently reported mammalian DGAT, and, to a lesser extent, to acyl CoA:cholesterol acyltransferases. Molecular analysis revealed that the mutant allele in AS11 has a 147 bp insertion located at the central ..
  51. ncbi Alteration of epitope recognition pattern in Ag85B and ESAT-6 has a profound influence on vaccine-induced protection against Mycobacterium tuberculosis
    Thomas Bennekov
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    Eur J Immunol 36:3346-55. 2006
    ....
  52. ncbi Overexpression of glycosylphosphatidylinositol (GPI) transamidase subunits phosphatidylinositol glycan class T and/or GPI anchor attachment 1 induces tumorigenesis and contributes to invasion in human breast cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Res 66:9829-36. 2006
    ..This aberrant growth is mediated, at least partially, by phosphorylation of paxillin, contributing to invasion and progression of breast cancer...
  53. ncbi Molecular engineering of resveratrol in plants
    Bertrand Delaunois
    Laboratory of Oenology and Applied Chemistry, Research Unit Vines and Wines of Champagne Stress and Environment, UPRES EA 2069, Faculty of Sciences, University of Reims, PO Box 1039, 51687 Reims Cedex 02, France
    Plant Biotechnol J 7:2-12. 2009
    ....
  54. pmc Escherichia coli K-12 Suppressor-free Mutants Lacking Early Glycosyltransferases and Late Acyltransferases: minimal lipopolysaccharide structure and induction of envelope stress response
    Gracjana Klein
    Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 22, D 23845 Borstel, Germany
    J Biol Chem 284:15369-89. 2009
    ..transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed...
  55. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
    ....
  56. pmc GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits
    Kisaburo Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 100:10682-7. 2003
    ....
  57. pmc Use of analogs and inhibitors to study the functional significance of protein palmitoylation
    Marilyn D Resh
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, Box 143, New York, NY 10021, USA
    Methods 40:191-7. 2006
    ..Finally, general approaches to determining the effect of altered palmitoylation status on YFPP association with membranes and lipid rafts, as well as signal transduction, are described...
  58. pmc Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1
    Daniel J Kliebenstein
    The Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Ithaca, New York 14853, USA
    Plant Physiol 130:234-43. 2002
    ..These results suggest that UVR8 acts in a UV-B signal transduction pathway leading to induction of flavonoid biosynthesis...
  59. ncbi Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria x ananassa)
    Stefan Lunkenbein
    Biomolecular Food Technology, Technical University Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 54:2145-53. 2006
    ....
  60. pmc PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system for pha genes
    M A Prieto
    Institute of Biotechnology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    J Bacteriol 181:858-68. 1999
    ..In addition, it has also been demonstrated that both PhaF and PhaI proteins are bound to PHA granules produced by P. oleovorans. A model for the role of PhaF in regulating PHA synthesis is presented...
  61. ncbi Yeast RNase III triggers polyadenylation-independent transcription termination
    Ghada Ghazal
    RNA Group, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
    Mol Cell 36:99-109. 2009
    ..These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals...
  62. pmc Seven functional classes of Barth syndrome mutation
    Kevin Whited
    Department of Physiology, Johns Hopkins School of Medicine, Baltimore, MD 21205 2185, USA
    Hum Mol Genet 22:483-92. 2013
    ..In sum, our in-depth characterization of the yeast BTHS-mutant panel has identified seven functional classes of BTHS mutation...
  63. pmc Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae
    Martin Pagac
    Department of Biology, University of Fribourg, CH 1700 Fribourg, Switzerland
    J Biol Chem 286:36438-47. 2011
    ..for all glycerophospholipids and triacylglycerols, is made de novo by the 1-acyl-sn-glycerol-3-phosphate acyltransferases Ale1p and Slc1p...
  64. pmc The growth reduction associated with repressed lignin biosynthesis in Arabidopsis thaliana is independent of flavonoids
    Xu Li
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Plant Cell 22:1620-32. 2010
    ....
  65. ncbi Effect of cold shock on lipid A biosynthesis in Escherichia coli. Induction At 12 degrees C of an acyltransferase specific for palmitoleoyl-acyl carrier protein
    S M Carty
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 274:9677-85. 1999
    ..The replacement of laurate with palmitoleate in lipid A may reflect the desirability of maintaining the optimal outer membrane fluidity at 12 degrees C...
  66. ncbi Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster
    Petra Spröte
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, 07745 Jena, Germany
    Mol Microbiol 70:445-61. 2008
    ..Our findings suggest that aatB is a paralogue of aatA. They extend the model of evolution of the penicillin biosynthesis gene cluster by recruitment of a biosynthesis gene and its cis-regulatory sites upon gene duplication...
  67. ncbi A recombinant adenovirus expressing immunodominant TB antigens can significantly enhance BCG-induced human immunity
    Daniel F Hoft
    Division of Infectious Diseases, Allergy and Immunology, Departments of Internal Medicine and Molecular Microbiology, Saint Louis University Medical Center and Center for Vaccine Development, Saint Louis, MO 63104, USA
    Vaccine 30:2098-108. 2012
    ..Despite the availability of Bacille Calmette Guérin (BCG) vaccines, Mycobacterium tuberculosis currently infects billions of people and millions die annually from tuberculosis (TB) disease. New TB vaccines are urgently needed...
  68. pmc Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-acyltransferase protein family
    Oliver Batistic
    Institut für Biologie und Biotechnologie der Pflanzen, Universitat Munster, 48149 Muenster, Germany
    Plant Physiol 160:1597-612. 2012
    ..This large concentration of plant PAT activity to the plasma membrane suggests that the plant cellular S-acylation machinery is functionally different compared with that of yeast (Saccharomyces cerevisiae) and mammalians...
  69. pmc Design and synthesis of inhibitors of Plasmodium falciparum N-myristoyltransferase, a promising target for antimalarial drug discovery
    Zhiyong Yu
    Department of Chemistry, Imperial College London, London SW7 2AZ, UK
    J Med Chem 55:8879-90. 2012
    ..These molecules provide a valuable starting point for further development...
  70. ncbi The M. tuberculosis antigen 85 complex and mycolyltransferase activity
    L Kremer
    Department of Microbiology and Immunology, University of Newcastle upon Tyne, UK
    Lett Appl Microbiol 34:233-7. 2002
    ..tuberculosis genome as FbpC1. An investigation was carried out to determine whether FbpC1 may also possess mycolyltransferase activity, a characteristic feature of the Ag85 complex...
  71. pmc Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populations
    Natalie E R Beveridge
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK
    Eur J Immunol 37:3089-100. 2007
    ..Overall, these data strongly support the use of MVA85A in humans as a boosting agent to expand polyfunctional M.tb-specific CD4(+) T cells capable of significant secondary responses...
  72. ncbi Distinct roles of Central missing and Dispatched in sending the Hedgehog signal
    K Amanai
    Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9133, USA
    Development 128:5119-27. 2001
    ..Finally, we show that cmn mutations bear molecular lesions in CG11495, which encodes a putative membrane bound acyltransferase related to Porcupine, a protein implicated in regulating the secretion of Wingless (Wg) signal...
  73. ncbi Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli
    Oksik Choi
    Korea Research Institute of Bioscience and Biotechnology, 685 1 Yangcheong ri Ochang eup, Chungbuk 363 883, Republic of Korea
    J Ind Microbiol Biotechnol 38:1657-65. 2011
    ....
  74. ncbi Phenotypic heterogeneity in body fat distribution in patients with congenital generalized lipodystrophy caused by mutations in the AGPAT2 or seipin genes
    Vinaya Simha
    Division of Nutrition and Metabolic Diseases, Center for Human Nutrition, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Clin Endocrinol Metab 88:5433-7. 2003
    ..We conclude that CGL patients with Seipin mutations have a more severe lack of body fat, which affects both metabolically active and mechanical adipose tissue, compared with patients with mutations in the AGPAT2 gene...
  75. pmc Considerable increase in resveratrol production by recombinant industrial yeast strains with use of rich medium
    Tobias Sydor
    Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany
    Appl Environ Microbiol 76:3361-3. 2010
    ..The use of rich medium considerably improved resveratrol production, and resveratrol yields of up to 391 mg/liter could be achieved with an industrial Brazilian sugar cane-fermenting yeast...
  76. ncbi GOAT: the master switch for the ghrelin system?
    Amparo Romero
    Department of Physiology, School of Medicine, University of Santiago de Compostela Instituto de Investigación Sanitaria, Santiago de Compostela, A Coruña 15782, Spain
    Eur J Endocrinol 163:1-8. 2010
    ....
  77. ncbi Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells
    Yechun Wang
    Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA
    J Biotechnol 157:258-60. 2012
    ..0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein. This work demonstrated the synthetic scaffolds can be used for the optimization of engineered metabolic pathway...
  78. pmc Novel hydroxycinnamoyl-coenzyme A quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acid
    Gabriella Sonnante
    Institute of Plant Genetics, National Research Council, 70126 Bari, Italy
    Plant Physiol 153:1224-38. 2010
    ..These findings suggest that several isoforms of HQT contribute to the synthesis of CGA in artichoke according to physiological needs and possibly following various metabolic routes...
  79. ncbi Coincident sequence-specific RNA degradation of linked transgenes in the plant genome
    Megumi Kasai
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Plant Mol Biol 78:259-73. 2012
    ..These results implicate the involvement of read-through transcription and distinct phases of RNA degradation in the coincident PTGS of linked transgenes and provide new insights into the destabilization of transgene expression...
  80. ncbi Ghrelin axis genes, peptides and receptors: recent findings and future challenges
    Inge Seim
    Queensland University of Technology, Brisbane, Queensland, Australia
    Mol Cell Endocrinol 340:3-9. 2011
    ..Studies of the role of ghrelin axis genes, peptides and receptors, therefore, promises to be a fruitful area of basic and clinical research in years to come...
  81. pmc Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 11:1523-33. 2000
    ..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment...
  82. pmc A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    J Lipid Res 46:2388-97. 2005
    Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids...
  83. pmc Biosynthesis of isoprenoid wax ester in Marinobacter hydrocarbonoclasticus DSM 8798: identification and characterization of isoprenoid coenzyme A synthetase and wax ester synthases
    Erik Holtzapple
    Department Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA
    J Bacteriol 189:3804-12. 2007
    ....
  84. ncbi Analysis of DHHC acyltransferases implies overlapping substrate specificity and a two-step reaction mechanism
    Haitong Hou
    University of Osnabruck, Department of Biology, Biochemistry Section, Osnabruck, Germany
    Traffic 10:1061-73. 2009
    Asp-His-His-Cys (DHHC) cysteine-rich domain (CRD) acyltransferases are polytopic transmembrane proteins that are found along the endomembrane system of eukaryotic cells and mediate palmitoylation of peripheral and integral membrane ..
  85. pmc Mutational analysis of Saccharomyces cerevisiae Erf2 reveals a two-step reaction mechanism for protein palmitoylation by DHHC enzymes
    David A Mitchell
    Department of Molecular Medicine, University of South Florida, Tampa, Florida 33612, USA
    J Biol Chem 285:38104-14. 2010
    DHHC protein acyltransferases (PATs) catalyze the palmitoylation of eukaryotic proteins through an enzymatic mechanism that remains largely unexplored...
  86. pmc Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis
    Ulf Stahl
    Department of Crop Science, Swedish University of Agricultural Sciences, S 230 53 Alnarp, Sweden
    Plant Physiol 135:1324-35. 2004
    ..However, these microsomes were still able to carry out triacylglycerol synthesis by a diacylglycerol:diacylglycerol acyltransferase reaction at the same rate as microsomal preparations from wild type...
  87. ncbi A polyvalent DNA vaccine expressing an ESAT6-Ag85B fusion protein protects mice against a primary infection with Mycobacterium tuberculosis and boosts BCG-induced protective immunity
    Steven C Derrick
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Building 29, Room 502, CBER FDA, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Vaccine 23:780-8. 2004
    ....
  88. ncbi Identification of the acyltransferase that octanoylates ghrelin, an appetite-stimulating peptide hormone
    Jing Yang
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell 132:387-96. 2008
    ..Analysis of the mouse genome revealed that GOAT belongs to a family of 16 hydrophobic membrane-bound acyltransferases that includes Porcupine, which attaches long-chain fatty acids to Wnt proteins...
  89. ncbi Stilbene compounds and stilbene synthase expression during ripening, wilting, and UV treatment in grape cv. Corvina
    A Versari
    Corso di Laurea in Scienze e Tecnologie Alimentari, , Via Ravennate 1020, Cesena (FC) 47023 Italy
    J Agric Food Chem 49:5531-6. 2001
    ..A time-course experiment showed the highest STS mRNA accumulation and resveratrol content (34 microg/g fresh weight at 58 days) occurring in berry skins in a mixed wilting process...
  90. pmc Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis
    Harrie van Erp
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
    Plant Physiol 155:683-93. 2011
    ..We show the usefulness of RcPDAT1A for engineering plants with high levels of HFAs and alleviating bottlenecks due to the production of unusual FAs in transgenic oilseeds...
  91. pmc Synthesis of novel lipids in Saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase
    Rainer Kalscheuer
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat, Munster, Germany
    Appl Environ Microbiol 70:7119-25. 2004
    ..encoding a DGAT (Dga1p), a phospholipid:diacylglycerol acyltransferase (Lro1p) and two acyl-coenzyme A:sterol acyltransferases (ASATs) (Are1p and Are2p) are involved in the final esterification steps in TAG and steryl ester biosynthesis ..
  92. ncbi Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily
    Aaron R Turkish
    Department of Pediatrics, Columbia University Medical Center, New York, New York 10032, USA
    J Biol Chem 280:14755-64. 2005
    ..mediate the synthesis of triacylglycerol; however, two of the X-linked genes act as acyl-CoA wax alcohol acyltransferases (AWAT 1 and 2) that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to ..
  93. ncbi Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorption
    Dong Cheng
    Pharmaceutical Research Institute, Bristol Myers Squibb Company, Princeton, New Jersey 08543, USA
    J Biol Chem 278:13611-4. 2003
    ..The molecular identification of MGAT3 will facilitate the evaluation of using intestinal MGAT as a potential point of intervention for antiobesity therapies...
  94. ncbi DHHC9 and GCP16 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras
    John T Swarthout
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:31141-8. 2005
    ..Hence, DHHC9.GCP16 displays the properties of a functional human ortholog of the yeast Ras palmitoyltransferase...
  95. ncbi Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote
    Bertrand Boisson
    Protein Maturation Group, Institut des Sciences du Vegetal, UPR2355, Centre National de la Recherche Scientifique, Batiment 23, 1 Avenue de la Terrasse, F 91198 Gif sur Yvette Cedex, France
    J Biol Chem 278:43418-29. 2003
    ..The role of N-myristoylation is discussed in each case; in particular, this process may underlie the "guard" hypothesis of innate immunity...
  96. ncbi Molecular symmetry in mitochondrial cardiolipins
    Michael Schlame
    Department of Anesthesiology, New York University School of Medicine, 550 First Ave, New York, NY 10016, USA
    Chem Phys Lipids 138:38-49. 2005
    ..This limits the structural diversity of the cardiolipin species and creates molecular symmetry with implications for the stereochemistry of cardiolipin...
  97. pmc Production of resveratrol in recombinant microorganisms
    Jules Beekwilder
    Plant Research International, 6700 AA Wageningen, The Netherlands
    Appl Environ Microbiol 72:5670-2. 2006
    ..When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter)...
  98. ncbi Transcriptional and posttranscriptional gene silencing are mechanistically related
    T Sijen
    Department of Developmental Genetics, Institute for Molecular Biological Sciences, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV, The, Amsterdam, Netherlands
    Curr Biol 11:436-40. 2001
    ....
  99. pmc Recent progress on acyl CoA: lysophospholipid acyltransferase research
    Hideo Shindou
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Lipid Res 50:S46-51. 2009
    ..from the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family, and also from the membrane bound O-acyltransferases (MBOAT) family. These discoveries have prompted a robust surge of research in this field...
  100. pmc Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 51:2153-70. 2010
    ..We believe this study provides a foundation to investigate cardiolipin metabolism and bioenergetic homeostasis in normal and disease states...
  101. pmc The presence of peptidoglycan O-acetyltransferase in various staphylococcal species correlates with lysozyme resistance and pathogenicity
    Agnieszka Bera
    Microbial Genetics, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Infect Immun 74:4598-604. 2006
    ..arlettae). In all lysozyme-sensitive species, the analyzed PG was de-O-acetylated. When we transformed the oatA gene from lysozyme-resistant S. aureus into S. carnosus, the corresponding transformants also became lysozyme resistant...

Research Grants63

  1. Chemoenzymatic synthesis of glycopeptide derivatives
    RYAN KRUGER; Fiscal Year: 2005
    ..The glycosyltransferases and acyltransferases from these two pathways have been recently identified...
  2. Structural biology of enzymes in fatty acid metabolism
    Liang Tong; Fiscal Year: 2012
    Acetyl-coenzyme A (acetyl-CoA) carboxylases (ACCs), pyruvate carboxylase (PC), carnitine acyltransferases, and AMP-activated protein kinase (AMPK) play crucial roles in the metabolism of fatty acids and/or carbohydrates, as well as other ..
  3. Regulation/Role of AcylCer in Normal Epidermis and Atopic Dermatitis
    Peter M Elias; Fiscal Year: 2013
    ..We recently showed that both acyl-CoA wax alcohol acyltransferases (AWAT) 1 and CGI-58 (a cofactor of triacylglycerol lipase) are further required for w-O-esterification, ..
  4. BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDES
    Pei Zhou; Fiscal Year: 2013
    ..They are: 1) mechanistic and structural studies of the acyltransferases LpxA and LpxD;2) the characterization of E...
  5. Regulation of Mitochondrial Dysfunction and Diet-Induced Obesity by ALCAT1
    Yuguang Shi; Fiscal Year: 2012
    ..by a "remodeling" process that involves deacylation by phospholipases and reacylation by lysocardiolipin acyltransferases. Defective CL remodeling in response to reactive oxygen species (ROS) leads to accumulation of bad CL and ..
  6. Acyl-CoA Synthetase: Structure, Function and Regulation
    ROSALIND ANNE COLEMAN; Fiscal Year: 2013
    ..at least 50% of the protein is present on the endoplasmic reticulum where it interacts with glycerolipid acyltransferases;and 3) that the mechanism for these differences lies both in the membrane association and phosphorylation ..
  7. 2013 Molecular and Cellular Biology of Lipids Gordon Research Conference
    ROSALIND ANNE COLEMAN; Fiscal Year: 2013
    ..a broad range of topics, including Lipid signals that regulate cell metabolism, New roles for lipases and acyltransferases, Membrane biogenesis and turnover, Cellular lipid metabolism, Fatty acid metabolism, Transcriptional control ..
  8. Molecular Mechanism of Barth Syndrome
    Michael Schlame; Fiscal Year: 2009
    ..The mutated gene (tafazzin) is homologous to a conserved family of phospholipid acyltransferases. Children with Barth syndrome are deficient in the mitochondria! phospholipid cardiolipin, suggesting that ..
  9. Glycine N-Acyltransferases
    David J Merkler; Fiscal Year: 2013
    ..We specifically propose to: (a) characterize a novel set of mammalian glycine N-acyltransferases (GLYATs) and define the role played by these enzymes in NAG biosynthesis and (b) determine the relative ..
  10. Global Characterization of Protein Palmitoylation in Trypanosomes
    David M Engman; Fiscal Year: 2013
    ..rarely N-palmitoylation (occurring on an N-terminal cys residue), is mediated by one of a number of palmitoyl acyltransferases (PATs), each having its own localization and substrate specificity...
  11. EFFECTS OF POLYUNSATURATED FATTY ACIDS ON MEGAKARYOCYTES
    PAUL SCHICK; Fiscal Year: 1991
    ..There is evidence desaturases and acyltransferases are sensitive to PUFA-modified diets...
  12. ENZYMES IN PHOSPHATIDIC ACID BIOSYNTHESIS
    Hei Sook Sul; Fiscal Year: 2002
    ..in a unique position to address, by biochemical and molecular biological approaches, the role of these two acyltransferases in the positional distribution of fatty acids in glycerophospholipids and in the regulation of lipid ..
  13. SHORT-CHAIN ACYLCARNITINES--FUNCTION AND ENZYMOLOGY
    LORAN BIEBER; Fiscal Year: 1993
    ..determination of partial amino acid sequences, and their properties will be compared to the other carnitine acyltransferases of liver...
  14. DIET AND CARCINOGENESIS BY PEROXISOME PROLIFERATORS
    HOWARD GLAUERT; Fiscal Year: 1993
    ..of several proteins, including the enzymes of the peroxisomal beta-oxidation pathway, the carnitine acyltransferases, and cytochrome P-450IVA1...
  15. Aging and Lipid Metaboilism
    CHRISTOPHER JOLLY; Fiscal Year: 2001
    ..The fatty acids have to be in the acyl-CoA form in order to be utilized by the acyltransferases. Kinetic studies will be conducted using oleoyl-CoA with or without albumin or ACBP present which increases ..
  16. STEROID DYNAMICS IN ENDOCRINOLOGY
    ERLIO GURPIDE; Fiscal Year: 1991
    ..and the effects of steroids, peptide hormones and calcium ionophore on the activities of phospholipases, acyltransferases, PG synthetase, and PG metabolizing enzymes...
  17. STRUCTURAL STUDIES OF A GROUP OF ACYLTRANSFERASES
    Donghai Wu; Fiscal Year: 2000
    ..project is to understand the structural basis of substrate recognition and catalytic mechanism for a group of acyltransferases. Based on sequence homology and reaction mechanism, these acyltransferases constitute a unique gene family ..
  18. LIPID DEPOSITION IN MAMMALIAN CELLS
    ARTHUR SPECTOR; Fiscal Year: 1980
    ..enzymes involved in lipid metabolism such as NADPH-dependent lipid peroxidation, fatty acid desaturases, acyltransferases involved in phospholipid and triglyceride synthesis and HMGCoA reductase...
  19. HITACHI ELECTRON MICROSCOPE
    Alvin Beitz; Fiscal Year: 1990
    ..Dr. Brady plans to use novel monoclonal antibodies against carnitine acyltransferases to analyze the location of these enzymes in the mitochondria and peroxisomes of liver cells.
  20. Lipid modification of secreted signaling proteins
    Jessica Treisman; Fiscal Year: 2009
    ..Palmitoylation catalyzed by the acyltransferases Rasp and Porcupine promotes the secretion, transport and signaling activity of Hedgehog and Wnt family ..
  21. PROTEIN PALMITOYLATION IN SIGNALING SYSTEMS
    Maurine E Linder; Fiscal Year: 2010
    ..The breakthrough in this field was our discovery of a large family of protein acyltransferases or PATs...
  22. Mutant Acyltransferases in Natural Product Biosynthesis
    ANDREW KOPPISCH; Fiscal Year: 2003
    ..This information is desirable for the rational design of efficiently biosynthesized unique polyketide metabolites. ..
  23. Inhibitors of Human Pal mitoyl Acyltransferase
    CHARLES DUCKER; Fiscal Year: 2005
    The goal of this program is to develop novel inhibitors of human palmitoyl acyltransferases (PATs) that are effective as cancer therapeutic agents...
  24. Barth Syndrome: Identifying the missing enzyme function
    TAL LEWIN; Fiscal Year: 2006
    ..have mutations in the tafazzin gene (TAZ) which belongs to a family of established and putative glycerolipid acyltransferases. Consistent with this finding is that tissues from BTHS patients contain decreased cardiolipin (CL), a ..
  25. FASEB Summer Conference, July 20-25, 2002
    Fuyuhiko Tamanoi; Fiscal Year: 2002
    ..thioacylation has been obtained, including the potential identification of the elusive first protein; fatty acyltransferases. Structural studies of the enzymes involved in protein prenylation and myristoylation have yield ..
  26. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2009
    ..Multicolor imaging with site-specific fluorescein derivatives (FLASH and ReAsH) will be used to determine the order in which Gag assembly sites are formed and maintained in the cell. ..
  27. Glial cell differentiation and glioma formation
    MARILYN RESH; Fiscal Year: 2006
    ..The activity of the above proteins in human glioma tumor samples will be determined. ..
  28. The Role of DGAT1 In Muscle Lipotoxicity
    Yi Hao Yu; Fiscal Year: 2006
    ..As a result, we may be able to identify new molecular target(s) for future drug therapy aimed at reducing lipotoxicity ..
  29. PURINE BIOSYNTHESIS ENZYMES-MECHANISMS AND REGULATION
    JoAnne Stubbe; Fiscal Year: 2003
    ..abstract_text> ..
  30. MECHANISM OF DRUG INDUCED DNA DEGRADATION
    JoAnne Stubbe; Fiscal Year: 2005
    ..The structures of these lesions will be determined. The repair of these lesions by Apel will be investigated in an effort to determine its relevance to the non-homologous end joining ds-break repair. ..
  31. Polyhydroxyalkanoates: A paradigm for non-template dependent polymerizations
    JoAnne Stubbe; Fiscal Year: 2009
    ..Understanding the roles of PhaC, PhaP and PhaZIa would be greatly enhanced with structural insight and efforts to crystallize proteins involved in PHB homeostasis will be actively pursued. ..
  32. DGAT Overexpression In Mouse Adipose Tissue and Muscle
    Yi Hao Yu; Fiscal Year: 2006
    ....
  33. Amine N-Oxygenation by FMO3 and FMO4
    John Cashman; Fiscal Year: 2005
    ..Such fundamental information will be useful in the development of safer drugs, the prevention of adverse drug reactions and the protection of humans from disease. ..
  34. MOLECULAR MECHANISMS IN ENDOTHELIAL DYSFUNCTION IN HIV 1
    John Ho; Fiscal Year: 2004
    ....
  35. THE PATHOGENESIS OF AUTOIMMUNITY IN A MURINE MODEL OF PRIMARY BILIARY CIRRHOSIS
    MERRILL E GERSHWIN; Fiscal Year: 2010
    ..abstract_text> ..
  36. MECHANISM & STRUCTURE OF HUMAN GAR TRANSFORMYLASE
    CAROL CAPERELLI; Fiscal Year: 2004
    ..Although it has been demonstrated that inhibition of this pathway shows potential for cancer chemotherapy, much basic information about the pathway and its constituent enzymes remains to be elucidated. ..
  37. Health and QOL among Long-Term Lung Cancer Survivors
    Ping Yang; Fiscal Year: 2010
    ....
  38. Vitamin A Storage and Metabolism
    William S Blaner; Fiscal Year: 2010
    ..Finally, in Aim 3 we will investigate how vitamin A is mobilized from HSCs and whether this requires direct involvement of serum retinol-binding protein (RBP) synthesized in hepatocytes. ..
  39. Inflammatory Biomarkers and Colorectal Cancer Risk
    Gong Yang; Fiscal Year: 2010
    ..Overall, this study will contribute significantly to the understanding of the role of inflammation in the etiology of CRC and to the development of new strategies for the assessment of CRC risk. ..
  40. Novel Therapies for Metabolic Complications in Patients with Lipodystrophies
    Abhimanyu Garg; Fiscal Year: 2010
    ..Additionally, the findings may be applicable to treating metabolic complications in patients with obesity and type 2 diabetes. ..
  41. Lipid Metabolism in the Aging Immune System
    CHRISTOPHER JOLLY; Fiscal Year: 2006
    ..abstract_text> ..
  42. Conference on Drug Against Tropical Protozoan Parasites
    Michael Gelb; Fiscal Year: 2002
    ..We consider the poster sessions that occur throughout our meeting to be just as important as the collection of lecture presentations. ..
  43. MODULATION OF DNA REPAIR BY P53 IN HUMAN UROEPITHELIUM
    Santhanam Swaminathan; Fiscal Year: 2001
    ..The results of these studies have important etiologic, mechanistic and therapeutic implications in human cancers. ..
  44. STRUCTURAL STUDIES ON RIBOSOMAL COMPONENTS
    Stephen White; Fiscal Year: 2002
    ..Future work will improve this image to accurately orient the IF3 molecule, and additional factors will be added to build up the complete initiation complex. ..
  45. 9.4 Tesla Fourier Transform Mass Spectrometer
    Stephen Barnes; Fiscal Year: 2002
    ..Overall, the FT-MS instrument will sustain the high quality of biomedical research that is a hallmark of UAB. ..
  46. ACYLTRANSFERASE INHIBITORS AS ANTI RAS AGENTS
    Charles Smith; Fiscal Year: 2009
    The overall goal of this program is to develop novel inhibitors of human protein acyltransferases (hPATs) that =re effective as cancer therapeutic agents...
  47. Development of DHPS as a Bioterrorism Therapeutic Target
    Stephen White; Fiscal Year: 2005
    ..The institutions have complementary programs in infectious diseases with a strong emphasis on studying bioterrorism. ..
  48. RNA Interference using gutless adenoviral vectors
    Nuria Morral; Fiscal Year: 2005
    ..Upon completion of our studies, the system could be used in a large number of research applications directed at studying gene function in this organ as well as many other tissues that can be transduced by adenoviral vectors. ..
  49. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2006
    ..These pathways are of direct significance to the biology of stress responses and specifically to cancer therapeutics. ..
  50. Ceramide in angiogenic integrin signaling
    Anat Erdreich Epstein; Fiscal Year: 2006
    ....
  51. Thiol-Reactive Crosslinkers for Biomaterials
    Glenn Prestwich; Fiscal Year: 2004
    ..Fourth, these new materials will be evaluated for cytocompatibility and cell growth in vitro with murine fibroblasts. Finally, selected materials will be seeded with fibroblasts and injected into nude mice for in vivo tissue generation. ..
  52. Therapeutic Approaches to HAART-Induced Lipodystrophy
    Abhimanyu Garg; Fiscal Year: 2006
    ..Additionally, the study may help prevent these problems in HIV-infected patients being initiated on PI-containing HAART. ..
  53. RPE65 IN RETINAL METABOLISM AND DEGENERATION
    Debra Thompson; Fiscal Year: 2005
    ..The long-term goals of this project are to elucidate the mechanisms by which RPE65 defects contribute to retinal degeneration, and to lay the groundwork for the development of therapeutic approaches to the disease. ..
  54. Carotene Cleavage Enzyme: Properties and Physiology
    WILLIAM BLANER; Fiscal Year: 2007
    ..In Aim 4, we propose to use a cDNA clone for human CCE to characterize the biochemical properties of the recombinant enzyme. ..
  55. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  56. MBD2 as a Target for Cancer Prevention and Treatment
    William Nelson; Fiscal Year: 2007
    ..abstract_text> ..
  57. REGENERATION OF 11-CIS-RETINAL IN THE RETINA
    Robert Rando; Fiscal Year: 2007
    ..Finally, we plan to synthesize novel antagonists of IMH to aid in understanding its physiological role(s). Specific IMH antagonists will also be used to limit the visual cycle in approaches to the treatment of macular degeneration. ..
  58. Chemopreventive Actions of Equol Enantiomers
    Kenneth Setchell; Fiscal Year: 2007
    ..These studies are relevant to humans given the ammended health claim for soy and cancer risk-reduction currently being reviewed by the FDA. ..
  59. The Glucoregulatory and Liporegulatory Peptide Hormones
    Roger Unger; Fiscal Year: 2008
    ..Using FA-depleted islets we have been able to "cure" insulin-deficient diabetes in rats for ~1 year without immunosuppression. [unreadable] [unreadable] [unreadable]..
  60. Brain tumor angiogenesis: Integrin ceramide signaling
    Anat Erdreich Epstein; Fiscal Year: 2008
    ..abstract_text> ..
  61. Radioimmunotherapy of CD20+ Lymphomas & CD45+Leukemias
    JOHN PAGEL; Fiscal Year: 2008
    ..abstract_text> ..
  62. Role of DAP12 in type 1 anti-mycobacterial immunity
    Zhou Xing; Fiscal Year: 2005
    ..We strongly believe that our studies will provide new insights into the mechanisms of type 1 anti-microbial immunity and novel targets for immune modulation. ..