Genomes and Genes
Summary: Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.
Publications273 found, 100 shown here
- Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plantsA Dahlqvist
Scandinavian Biotechnology Research ScanBi AB, Herman Ehles Väg 2 S 26831 Svalôv, Sweden
Proc Natl Acad Sci U S A 97:6487-92. 2000..However, budding yeast PDAT and its relatives in fission yeast and Arabidopsis form a distinct branch within this protein superfamily, indicating that a separate PDAT enzyme arose at an early point in evolution...
- A fluorescence-based assay for N-myristoyltransferase activityVictor Goncalves
Department of Chemistry, Imperial College London, London SW7 2AZ, UK
Anal Biochem 421:342-4. 2012..We also describe expression and characterization of NMT1 and NMT2 and assay validation with small molecule inhibitors. This assay should be broadly applicable to NMTs from a range of organisms...
- Ghrelin octanoylation mediated by an orphan lipid transferaseJesus A Gutierrez
Integrative Biology, Eli Lilly and Company, 2001 West Main Street, Greenfield, IN 46140, USA
Proc Natl Acad Sci U S A 105:6320-5. 2008..The occurrence of ghrelin and GOAT in stomach and pancreas tissues demonstrates the relevance of GOAT in the acylation of ghrelin and further implicates acylated ghrelin in pancreatic function...
- Global analysis of protein palmitoylation in yeastAmy F Roth
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
Cell 125:1003-13. 2006..e., protein acyltransferases (PATs)--were identified only recently...
- DHHC palmitoyl transferases: substrate interactions and (patho)physiologyJennifer Greaves
Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK
Trends Biochem Sci 36:245-53. 2011..Recent studies have highlighted how DHHC proteins regulate cell function and influence physiology and pathophysiology...
- N-myristoyltransferase inhibitors as new leads to treat sleeping sicknessJulie A Frearson
Drug Discovery Unit, Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee DD1 5EH, UK
Nature 464:728-32. 2010..Our studies validate T. brucei N-myristoyltransferase as a promising therapeutic target for human African trypanosomiasis...
- Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage qualityGail Shadle
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Phytochemistry 68:1521-9. 2007..Although manipulation of lignin biosynthesis can greatly improve forage digestibility, accompanying effects on plant development need to be better understood...
- Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and functionRoshni R Singaraja
950 West 28th Ave, Vancouver, BC, V5Z 4H4, Canada
Circ Res 105:138-47. 2009..Thus, palmitoylation regulates ABCA1 localization at the plasma membrane, and regulates its lipid efflux ability...
- Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in ChlamydomonasNanette R Boyle
Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
J Biol Chem 287:15811-25. 2012..Three genes encoding acyltransferases, DGAT1, DGTT1, and PDAT1, are induced by nitrogen starvation and are likely to have a role in TAG ..
- Storage lipid synthesis is non-essential in yeastLine Sandager
Department of Crop Science, Swedish University of Agricultural Sciences, Box 44, S 230 53 Alnarp, Sweden
J Biol Chem 277:6478-82. 2002..The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies. We conclude that neither storage lipids nor lipid bodies are essential for growth in yeast...
- Mechanism of endophilin N-BAR domain-mediated membrane curvatureJennifer L Gallop
MRC Laboratory of Molecular Biology, Cambridge, UK
EMBO J 25:2898-910. 2006..Its presence defines an endophilin/nadrin subclass of BAR domains. We propose that N-BAR domains function as low-affinity dimers regulating binding partner recruitment to areas of high membrane curvature...
- Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesisJ T Belisle
Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA
Science 276:1420-2. 1997..Furthermore, the use of an antagonist (6-azido-6-deoxy-alpha, alpha'-trehalose) of this activity demonstrates that these proteins are essential and potential targets for new antimycobacterial drugs...
- Ag85B-ESAT-6 adjuvanted with IC31 promotes strong and long-lived Mycobacterium tuberculosis specific T cell responses in naïve human volunteersJaap T van Dissel
Leiden University Medical Center, Department of Infectious Diseases, Leiden, The Netherlands
Vaccine 28:3571-81. 2010..These strong responses persisted through 2.5 years of follow-up, indicating the induction of a substantial memory response in the vaccine recipients...
- Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesisS Cases
Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94143, USA
Proc Natl Acad Sci U S A 95:13018-23. 1998..The identification of this cDNA encoding a DGAT will greatly facilitate studies of cellular glycerolipid metabolism and its regulation...
- GOAT links dietary lipids with the endocrine control of energy balanceHenriette Kirchner
Departments of Psychiatry and Medicine, Obesity Research Centre and Genome Research Institute, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
Nat Med 15:741-5. 2009..These data implicate the ghrelin-GOAT system as a signaling pathway that alerts the central nervous system to the presence of dietary calories, rather than to their absence as is commonly accepted...
- Glucose and weight control in mice with a designed ghrelin O-acyltransferase inhibitorBrad P Barnett
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Science 330:1689-92. 2010..In addition to serving as a research tool for mapping ghrelin actions, GO-CoA-Tat may help pave the way for clinical targeting of GOAT in metabolic diseases...
- A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1Rainer Kalscheuer
Institut fur Mikrobiologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, Germany
J Biol Chem 278:8075-82. 2003..calcoaceticus. This novel type of long-chain acyl-CoA acyltransferase is not related to known acyltransferases including the WS from jojoba (Simmondsia chinensis), the DGAT1 or DGAT2 families present in yeast, plants, ..
- Discovery of Plasmodium vivax N-myristoyltransferase inhibitors: screening, synthesis, and structural characterization of their binding modeVictor Goncalves
Department of Chemistry, Imperial College London, London SW7 2AZ, United Kingdom
J Med Chem 55:3578-82. 2012..A set of analogues was designed and tested to define the chemical groups relevant for activity and selectivity...
- Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesisLaurent Hoffmann
Institut de Biologie Moleculaire des Plantes, Unite Propre de Recherche, 2357 du Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67000 Strasbourg, France
Plant Cell 16:1446-65. 2004..These results demonstrate the function of the acyltransferase in phenylpropanoid biosynthesis...
- Enhanced immunogenicity and protective efficacy against Mycobacterium tuberculosis of bacille Calmette-Guérin vaccine using mucosal administration and boosting with a recombinant modified vaccinia virus AnkaraNilu P Goonetilleke
Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford, United Kingdom
J Immunol 171:1602-9. 2003..These findings support further evaluation of mucosally targeted prime-boost vaccination approaches for tuberculosis...
- Acyltransferases in plants: a good time to be BAHDJohn C D'Auria
Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, D 07745 Jena, Germany
Curr Opin Plant Biol 9:331-40. 2006Acylation is a common and biochemically significant modification of plant secondary metabolites. Plant BAHD acyltransferases constitute a large family of acyl CoA-utilizing enzymes whose products include small volatile esters, modified ..
- Neutral lipid biosynthesis in engineered Escherichia coli: jojoba oil-like wax esters and fatty acid butyl estersRainer Kalscheuer
Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat, Corrensstrasse 3, D 48149 Munster, Germany
Appl Environ Microbiol 72:1373-9. 2006..coli, which might open new perspectives for the biotechnological production of cheap jojoba oil equivalents from inexpensive resources employing recombinant microorganisms...
- Palmitoylation of intracellular signaling proteins: regulation and functionJessica E Smotrys
Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Annu Rev Biochem 73:559-87. 2004..The regulation of palmitoylation occurs through the actions of protein acyltransferases and protein acylthioesterases...
- Protein palmitoylation by a family of DHHC protein S-acyltransferasesDavid A Mitchell
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA
J Lipid Res 47:1118-27. 2006..This review presents the genetic and biochemical evidence for the PAT activity of DHHC proteins and discusses the mechanism of protein-mediated palmitoylation...
- Ghrelin O-acyltransferase (GOAT) is essential for growth hormone-mediated survival of calorie-restricted miceTong Jin Zhao
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 107:7467-72. 2010..Thus, an essential function of ghrelin in mice is elevation of GH levels during severe calorie restriction, thereby preserving blood glucose and preventing death...
- Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in cultureJaiyanth Daniel
University of Central Florida, Biomolecular Science Center, BMS 136, 4000 Central Florida Blvd, Orlando, FL 32816 2364, USA
J Bacteriol 186:5017-30. 2004..Therefore, TG synthesis may be an appropriate target for novel antilatency drugs that can prevent the organism from surviving dormancy and thus assist in the control of tuberculosis...
- Phytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolismPhilippe Jeandet
Laboratoire d Oenologie, URVVC, UPRES EA 2069, Faculte des Sciences, Universite de Reims, B P 1039, 51687 Reims Cedex 02, France
J Agric Food Chem 50:2731-41. 2002....
- Single mucosal, but not parenteral, immunization with recombinant adenoviral-based vaccine provides potent protection from pulmonary tuberculosisJun Wang
Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Centre for Gene Therapeutics, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
J Immunol 173:6357-65. 2004..Our study also lends strong evidence that respiratory mucosal vaccination is critically advantageous over systemic routes of vaccination against TB...
- N-myristoyltransferase from Leishmania donovani: structural and functional characterisation of a potential drug target for visceral leishmaniasisJames A Brannigan
York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5YW, UK
J Mol Biol 396:985-99. 2010..These studies validate L. donovani NMT as a potential target for development of new therapeutic agents against visceral leishmaniasis...
- DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed developmentMeng Zhang
Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
Plant Cell 21:3885-901. 2009....
- Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteinsYusuke Ohno
Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 jo, Nishi 6 choume, Kita ku, Sapporo 060 0812, Japan
Biochim Biophys Acta 1761:474-83. 2006Increasing evidence indicates that DHHC cysteine-rich domain-containing proteins (DHHC proteins) are protein acyltransferases. Although multiple DHHC proteins are found in eukaryotes, characterization has been examined for only a few...
- A superfamily of membrane-bound O-acyltransferases with implications for wnt signalingK Hofmann
Bioinformatics and Gene Discovery Group, MEMOREC Stoffel GmbH, Stöckheimer Weg 1, D 50829 Köln, Germany
Trends Biochem Sci 25:111-2. 2000
- Poly(3-hydroxybutyrate) granule-associated proteins: impacts on poly(3-hydroxybutyrate) synthesis and degradationMarkus Pötter
Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, 48149 Munster, Germany
Biomacromolecules 6:552-60. 2005..In addition, putative applications of PHA granules and PHA granule-associated proteins in nanotechnology are discussed...
- Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic arabidopsisK D Lardizabal
Calgene Campus, Monsanto, 1920 Fifth Street, Davis, California 95616, USA
Plant Physiol 122:645-55. 2000..Gas chromatography analysis of transmethylated oil from individual seeds suggested that wax levels may represent up to 70% (by weight) of the oil present in those seeds...
- Dynamic protein palmitoylation in cellular signalingTsuyoshi Iwanaga
Division of Membrane Physiology, Department of Cell Physiology, National Institute for Physiological Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
Prog Lipid Res 48:117-27. 2009..Integrated approaches including advanced proteomics, live-cell imaging, and molecular genetics are beginning to clarify the molecular machinery for palmitoylation reaction in diverse aspects of cellular functions...
- AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34Anil K Agarwal
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390, USA
Nat Genet 31:21-3. 2002..AGPAT2 mRNA is highly expressed in adipose tissue. We conclude that mutations in AGPAT2 may cause congenital generalized lipodystrophy by inhibiting triacylglycerol synthesis and storage in adipocytes...
- A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeastP Oelkers
Institute of Human Nutrition, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
J Biol Chem 275:15609-12. 2000..Saccharomyces cerevisiae, we investigated five candidate genes with sequence conservation to mammalian acyltransferases. Four of these genes are similar to the recently identified acyl-CoA diacylglycerol acyltransferase and, when ..
- Multifunctional, high-level cytokine-producing Th1 cells in the lung, but not spleen, correlate with protection against Mycobacterium tuberculosis aerosol challenge in miceEmily K Forbes
The Jenner Institute, University of Oxford, Headington, Oxford, United Kingdom
J Immunol 181:4955-64. 2008..Successful immunization regimes appear to induce Ag-specific cells with abundant intracellular cytokine staining...
- Recent advances in the biosynthesis and accumulation of anthocyaninsKarin Springob
Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
Nat Prod Rep 20:288-303. 2003..The literature from 1997 to the beginning of 2002 is reviewed, and 163 references are cited...
- Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferaseP Bouvier-Nave
Institut de Biologie Moleculaire des Plantes, Strasbourg, France
Eur J Biochem 267:85-96. 2000During the course of a search for cDNAs encoding plant sterol acyltransferases, an expressed sequence tag clone presenting substantial identity with yeast and animal acyl CoA:cholesterol acyltransferases was used to screen cDNA libraries ..
- Cardiolipin remodeling by ALCAT1 links oxidative stress and mitochondrial dysfunction to obesityJia Li
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
Cell Metab 12:154-65. 2010..Collectively, these findings identify a key role of ALCAT1 in regulating CL remodeling, mitochondrial dysfunction, and susceptibility to DIO...
- Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolismLaurent Hoffmann
Institut de Biologie Moleculaire des Plantes, CNRS, Universite Louis Pasteur, 12, rue du Général Zimmer, 67000 Strasbourg, France
J Biol Chem 278:95-103. 2003..Thus, hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase appears to control the biosynthesis and turnover of major plant phenolic compounds such as lignin and chlorogenic acid...
- Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme familyJeffrey B Cheng
Department of Molecular Genetics, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Biol Chem 279:37798-807. 2004..little or no ability to synthesize cholesteryl esters, diacylglycerols, or triacylglycerols, whereas other acyltransferases, including the acyl-CoA:monoacylglycerol acyltransferase 1 and 2 enzymes and the acyl-CoA:diacylglycerol ..
- Deficiency of the intestinal enzyme acyl CoA:monoacylglycerol acyltransferase-2 protects mice from metabolic disorders induced by high-fat feedingChi Liang Eric Yen
Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA
Nat Med 15:442-6. 2009....
- Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurateT Clementz
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 271:12095-102. 1996..Transfer of palmitate, palmitoleate, or R-3-hydroxymyristate was very slow. Taken together with previous studies, our findings indicate that htrB encodes a key, late functioning acyltransferase of lipid A biosynthesis...
- Human CD8+ CTL specific for the mycobacterial major secreted antigen 85AS M Smith
Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom
J Immunol 165:7088-95. 2000..These results demonstrate a previously unrecognized, MHC class I-restricted, CD8(+) CTL response to a major secreted Ag of mycobacteria and supports the use of Ag85A as a candidate vaccine against tuberculosis...
- Malleability of the folding mechanism of the outer membrane protein PagP: parallel pathways and the effect of membrane elasticityGerard H M Huysmans
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
J Mol Biol 416:453-64. 2012..The results highlight the effect of lipid composition in tailoring the folding mechanism of a membrane protein, revealing that membrane proteins have access to multiple, competing folding routes to a unique native structure...
- Lecithin-retinol acyltransferase is essential for accumulation of all-trans-retinyl esters in the eye and in the liverMatthew L Batten
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:10422-32. 2004..We conclude that Lrat-/- mice may serve as an animal model with early onset severe retinal dystrophy and severe retinyl ester deprivation...
- Selective inhibitors of protozoan protein N-myristoyltransferases as starting points for tropical disease medicinal chemistry programsAndrew S Bell
Worldwide Medicinal Chemistry, Pfizer Worldwide Research and Development, Kent, UK
PLoS Negl Trop Dis 6:e1625. 2012....
- The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase geneJ Zou
National Research Council of Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada
Plant J 19:645-53. 1999..which is similar to a recently reported mammalian DGAT, and, to a lesser extent, to acyl CoA:cholesterol acyltransferases. Molecular analysis revealed that the mutant allele in AS11 has a 147 bp insertion located at the central ..
- Alteration of epitope recognition pattern in Ag85B and ESAT-6 has a profound influence on vaccine-induced protection against Mycobacterium tuberculosisThomas Bennekov
Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
Eur J Immunol 36:3346-55. 2006....
- Overexpression of glycosylphosphatidylinositol (GPI) transamidase subunits phosphatidylinositol glycan class T and/or GPI anchor attachment 1 induces tumorigenesis and contributes to invasion in human breast cancerGuojun Wu
Department of Otolaryngology Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Cancer Res 66:9829-36. 2006..This aberrant growth is mediated, at least partially, by phosphorylation of paxillin, contributing to invasion and progression of breast cancer...
- Molecular engineering of resveratrol in plantsBertrand Delaunois
Laboratory of Oenology and Applied Chemistry, Research Unit Vines and Wines of Champagne Stress and Environment, UPRES EA 2069, Faculty of Sciences, University of Reims, PO Box 1039, 51687 Reims Cedex 02, France
Plant Biotechnol J 7:2-12. 2009....
- Escherichia coli K-12 Suppressor-free Mutants Lacking Early Glycosyltransferases and Late Acyltransferases: minimal lipopolysaccharide structure and induction of envelope stress responseGracjana Klein
Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 22, D 23845 Borstel, Germany
J Biol Chem 284:15369-89. 2009..transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed...
- Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activitiesChristopher M Jenkins
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 279:48968-75. 2004....
- GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunitsKisaburo Nagamune
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
Proc Natl Acad Sci U S A 100:10682-7. 2003....
- Use of analogs and inhibitors to study the functional significance of protein palmitoylationMarilyn D Resh
Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, Box 143, New York, NY 10021, USA
Methods 40:191-7. 2006..Finally, general approaches to determining the effect of altered palmitoylation status on YFPP association with membranes and lipid rafts, as well as signal transduction, are described...
- Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1Daniel J Kliebenstein
The Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Ithaca, New York 14853, USA
Plant Physiol 130:234-43. 2002..These results suggest that UVR8 acts in a UV-B signal transduction pathway leading to induction of flavonoid biosynthesis...
- Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria x ananassa)Stefan Lunkenbein
Biomolecular Food Technology, Technical University Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
J Agric Food Chem 54:2145-53. 2006....
- PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system for pha genesM A Prieto
Institute of Biotechnology, ETH Honggerberg, CH 8093 Zurich, Switzerland
J Bacteriol 181:858-68. 1999..In addition, it has also been demonstrated that both PhaF and PhaI proteins are bound to PHA granules produced by P. oleovorans. A model for the role of PhaF in regulating PHA synthesis is presented...
- Yeast RNase III triggers polyadenylation-independent transcription terminationGhada Ghazal
RNA Group, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Mol Cell 36:99-109. 2009..These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals...
- Seven functional classes of Barth syndrome mutationKevin Whited
Department of Physiology, Johns Hopkins School of Medicine, Baltimore, MD 21205 2185, USA
Hum Mol Genet 22:483-92. 2013..In sum, our in-depth characterization of the yeast BTHS-mutant panel has identified seven functional classes of BTHS mutation...
- Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiaeMartin Pagac
Department of Biology, University of Fribourg, CH 1700 Fribourg, Switzerland
J Biol Chem 286:36438-47. 2011..for all glycerophospholipids and triacylglycerols, is made de novo by the 1-acyl-sn-glycerol-3-phosphate acyltransferases Ale1p and Slc1p...
- The growth reduction associated with repressed lignin biosynthesis in Arabidopsis thaliana is independent of flavonoidsXu Li
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
Plant Cell 22:1620-32. 2010....
- Effect of cold shock on lipid A biosynthesis in Escherichia coli. Induction At 12 degrees C of an acyltransferase specific for palmitoleoyl-acyl carrier proteinS M Carty
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 274:9677-85. 1999..The replacement of laurate with palmitoleate in lipid A may reflect the desirability of maintaining the optimal outer membrane fluidity at 12 degrees C...
- Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene clusterPetra Spröte
Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, 07745 Jena, Germany
Mol Microbiol 70:445-61. 2008..Our findings suggest that aatB is a paralogue of aatA. They extend the model of evolution of the penicillin biosynthesis gene cluster by recruitment of a biosynthesis gene and its cis-regulatory sites upon gene duplication...
- A recombinant adenovirus expressing immunodominant TB antigens can significantly enhance BCG-induced human immunityDaniel F Hoft
Division of Infectious Diseases, Allergy and Immunology, Departments of Internal Medicine and Molecular Microbiology, Saint Louis University Medical Center and Center for Vaccine Development, Saint Louis, MO 63104, USA
Vaccine 30:2098-108. 2012..Despite the availability of Bacille Calmette Guérin (BCG) vaccines, Mycobacterium tuberculosis currently infects billions of people and millions die annually from tuberculosis (TB) disease. New TB vaccines are urgently needed...
- Genomics and localization of the Arabidopsis DHHC-cysteine-rich domain S-acyltransferase protein familyOliver Batistic
Institut für Biologie und Biotechnologie der Pflanzen, Universitat Munster, 48149 Muenster, Germany
Plant Physiol 160:1597-612. 2012..This large concentration of plant PAT activity to the plasma membrane suggests that the plant cellular S-acylation machinery is functionally different compared with that of yeast (Saccharomyces cerevisiae) and mammalians...
- Design and synthesis of inhibitors of Plasmodium falciparum N-myristoyltransferase, a promising target for antimalarial drug discoveryZhiyong Yu
Department of Chemistry, Imperial College London, London SW7 2AZ, UK
J Med Chem 55:8879-90. 2012..These molecules provide a valuable starting point for further development...
- The M. tuberculosis antigen 85 complex and mycolyltransferase activityL Kremer
Department of Microbiology and Immunology, University of Newcastle upon Tyne, UK
Lett Appl Microbiol 34:233-7. 2002..tuberculosis genome as FbpC1. An investigation was carried out to determine whether FbpC1 may also possess mycolyltransferase activity, a characteristic feature of the Ag85 complex...
- Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populationsNatalie E R Beveridge
Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Eur J Immunol 37:3089-100. 2007..Overall, these data strongly support the use of MVA85A in humans as a boosting agent to expand polyfunctional M.tb-specific CD4(+) T cells capable of significant secondary responses...
- Distinct roles of Central missing and Dispatched in sending the Hedgehog signalK Amanai
Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9133, USA
Development 128:5119-27. 2001..Finally, we show that cmn mutations bear molecular lesions in CG11495, which encodes a putative membrane bound acyltransferase related to Porcupine, a protein implicated in regulating the secretion of Wingless (Wg) signal...
- Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coliOksik Choi
Korea Research Institute of Bioscience and Biotechnology, 685 1 Yangcheong ri Ochang eup, Chungbuk 363 883, Republic of Korea
J Ind Microbiol Biotechnol 38:1657-65. 2011....
- Phenotypic heterogeneity in body fat distribution in patients with congenital generalized lipodystrophy caused by mutations in the AGPAT2 or seipin genesVinaya Simha
Division of Nutrition and Metabolic Diseases, Center for Human Nutrition, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Clin Endocrinol Metab 88:5433-7. 2003..We conclude that CGL patients with Seipin mutations have a more severe lack of body fat, which affects both metabolically active and mechanical adipose tissue, compared with patients with mutations in the AGPAT2 gene...
- Considerable increase in resveratrol production by recombinant industrial yeast strains with use of rich mediumTobias Sydor
Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany
Appl Environ Microbiol 76:3361-3. 2010..The use of rich medium considerably improved resveratrol production, and resveratrol yields of up to 391 mg/liter could be achieved with an industrial Brazilian sugar cane-fermenting yeast...
- GOAT: the master switch for the ghrelin system?Amparo Romero
Department of Physiology, School of Medicine, University of Santiago de Compostela Instituto de Investigación Sanitaria, Santiago de Compostela, A Coruña 15782, Spain
Eur J Endocrinol 163:1-8. 2010....
- Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cellsYechun Wang
Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA
J Biotechnol 157:258-60. 2012..0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein. This work demonstrated the synthetic scaffolds can be used for the optimization of engineered metabolic pathway...
- Novel hydroxycinnamoyl-coenzyme A quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acidGabriella Sonnante
Institute of Plant Genetics, National Research Council, 70126 Bari, Italy
Plant Physiol 153:1224-38. 2010..These findings suggest that several isoforms of HQT contribute to the synthesis of CGA in artichoke according to physiological needs and possibly following various metabolic routes...
- Coincident sequence-specific RNA degradation of linked transgenes in the plant genomeMegumi Kasai
Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
Plant Mol Biol 78:259-73. 2012..These results implicate the involvement of read-through transcription and distinct phases of RNA degradation in the coincident PTGS of linked transgenes and provide new insights into the destabilization of transgene expression...
- Ghrelin axis genes, peptides and receptors: recent findings and future challengesInge Seim
Queensland University of Technology, Brisbane, Queensland, Australia
Mol Cell Endocrinol 340:3-9. 2011..Studies of the role of ghrelin axis genes, peptides and receptors, therefore, promises to be a fruitful area of basic and clinical research in years to come...
- Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteinsK Ohishi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
Mol Biol Cell 11:1523-33. 2000..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment...
- A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl estersChi Liang Eric Yen
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
J Lipid Res 46:2388-97. 2005Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids...
- Biosynthesis of isoprenoid wax ester in Marinobacter hydrocarbonoclasticus DSM 8798: identification and characterization of isoprenoid coenzyme A synthetase and wax ester synthasesErik Holtzapple
Department Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA
J Bacteriol 189:3804-12. 2007....
- Analysis of DHHC acyltransferases implies overlapping substrate specificity and a two-step reaction mechanismHaitong Hou
University of Osnabruck, Department of Biology, Biochemistry Section, Osnabruck, Germany
Traffic 10:1061-73. 2009Asp-His-His-Cys (DHHC) cysteine-rich domain (CRD) acyltransferases are polytopic transmembrane proteins that are found along the endomembrane system of eukaryotic cells and mediate palmitoylation of peripheral and integral membrane ..
- Mutational analysis of Saccharomyces cerevisiae Erf2 reveals a two-step reaction mechanism for protein palmitoylation by DHHC enzymesDavid A Mitchell
Department of Molecular Medicine, University of South Florida, Tampa, Florida 33612, USA
J Biol Chem 285:38104-14. 2010DHHC protein acyltransferases (PATs) catalyze the palmitoylation of eukaryotic proteins through an enzymatic mechanism that remains largely unexplored...
- Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from ArabidopsisUlf Stahl
Department of Crop Science, Swedish University of Agricultural Sciences, S 230 53 Alnarp, Sweden
Plant Physiol 135:1324-35. 2004..However, these microsomes were still able to carry out triacylglycerol synthesis by a diacylglycerol:diacylglycerol acyltransferase reaction at the same rate as microsomal preparations from wild type...
- A polyvalent DNA vaccine expressing an ESAT6-Ag85B fusion protein protects mice against a primary infection with Mycobacterium tuberculosis and boosts BCG-induced protective immunitySteven C Derrick
Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Building 29, Room 502, CBER FDA, 29 Lincoln Drive, Bethesda, MD 20892, USA
Vaccine 23:780-8. 2004....
- Identification of the acyltransferase that octanoylates ghrelin, an appetite-stimulating peptide hormoneJing Yang
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Cell 132:387-96. 2008..Analysis of the mouse genome revealed that GOAT belongs to a family of 16 hydrophobic membrane-bound acyltransferases that includes Porcupine, which attaches long-chain fatty acids to Wnt proteins...
- Stilbene compounds and stilbene synthase expression during ripening, wilting, and UV treatment in grape cv. CorvinaA Versari
Corso di Laurea in Scienze e Tecnologie Alimentari, , Via Ravennate 1020, Cesena (FC) 47023 Italy
J Agric Food Chem 49:5531-6. 2001..A time-course experiment showed the highest STS mRNA accumulation and resveratrol content (34 microg/g fresh weight at 58 days) occurring in berry skins in a mixed wilting process...
- Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic ArabidopsisHarrie van Erp
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
Plant Physiol 155:683-93. 2011..We show the usefulness of RcPDAT1A for engineering plants with high levels of HFAs and alleviating bottlenecks due to the production of unusual FAs in transgenic oilseeds...
- Synthesis of novel lipids in Saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferaseRainer Kalscheuer
Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat, Munster, Germany
Appl Environ Microbiol 70:7119-25. 2004..encoding a DGAT (Dga1p), a phospholipid:diacylglycerol acyltransferase (Lro1p) and two acyl-coenzyme A:sterol acyltransferases (ASATs) (Are1p and Are2p) are involved in the final esterification steps in TAG and steryl ester biosynthesis ..
- Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamilyAaron R Turkish
Department of Pediatrics, Columbia University Medical Center, New York, New York 10032, USA
J Biol Chem 280:14755-64. 2005..mediate the synthesis of triacylglycerol; however, two of the X-linked genes act as acyl-CoA wax alcohol acyltransferases (AWAT 1 and 2) that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to ..
- Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorptionDong Cheng
Pharmaceutical Research Institute, Bristol Myers Squibb Company, Princeton, New Jersey 08543, USA
J Biol Chem 278:13611-4. 2003..The molecular identification of MGAT3 will facilitate the evaluation of using intestinal MGAT as a potential point of intervention for antiobesity therapies...
- DHHC9 and GCP16 constitute a human protein fatty acyltransferase with specificity for H- and N-RasJohn T Swarthout
Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 280:31141-8. 2005..Hence, DHHC9.GCP16 displays the properties of a functional human ortholog of the yeast Ras palmitoyltransferase...
- Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryoteBertrand Boisson
Protein Maturation Group, Institut des Sciences du Vegetal, UPR2355, Centre National de la Recherche Scientifique, Batiment 23, 1 Avenue de la Terrasse, F 91198 Gif sur Yvette Cedex, France
J Biol Chem 278:43418-29. 2003..The role of N-myristoylation is discussed in each case; in particular, this process may underlie the "guard" hypothesis of innate immunity...
- Molecular symmetry in mitochondrial cardiolipinsMichael Schlame
Department of Anesthesiology, New York University School of Medicine, 550 First Ave, New York, NY 10016, USA
Chem Phys Lipids 138:38-49. 2005..This limits the structural diversity of the cardiolipin species and creates molecular symmetry with implications for the stereochemistry of cardiolipin...
- Production of resveratrol in recombinant microorganismsJules Beekwilder
Plant Research International, 6700 AA Wageningen, The Netherlands
Appl Environ Microbiol 72:5670-2. 2006..When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter)...
- Transcriptional and posttranscriptional gene silencing are mechanistically relatedT Sijen
Department of Developmental Genetics, Institute for Molecular Biological Sciences, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV, The, Amsterdam, Netherlands
Curr Biol 11:436-40. 2001....
- Recent progress on acyl CoA: lysophospholipid acyltransferase researchHideo Shindou
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
J Lipid Res 50:S46-51. 2009..from the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family, and also from the membrane bound O-acyltransferases (MBOAT) family. These discoveries have prompted a robust surge of research in this field...
- Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomicsMichael A Kiebish
Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
J Lipid Res 51:2153-70. 2010..We believe this study provides a foundation to investigate cardiolipin metabolism and bioenergetic homeostasis in normal and disease states...
- The presence of peptidoglycan O-acetyltransferase in various staphylococcal species correlates with lysozyme resistance and pathogenicityAgnieszka Bera
Microbial Genetics, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
Infect Immun 74:4598-604. 2006..arlettae). In all lysozyme-sensitive species, the analyzed PG was de-O-acetylated. When we transformed the oatA gene from lysozyme-resistant S. aureus into S. carnosus, the corresponding transformants also became lysozyme resistant...
- Chemoenzymatic synthesis of glycopeptide derivativesRYAN KRUGER; Fiscal Year: 2005..The glycosyltransferases and acyltransferases from these two pathways have been recently identified...
- Structural biology of enzymes in fatty acid metabolismLiang Tong; Fiscal Year: 2012Acetyl-coenzyme A (acetyl-CoA) carboxylases (ACCs), pyruvate carboxylase (PC), carnitine acyltransferases, and AMP-activated protein kinase (AMPK) play crucial roles in the metabolism of fatty acids and/or carbohydrates, as well as other ..
- Regulation/Role of AcylCer in Normal Epidermis and Atopic DermatitisPeter M Elias; Fiscal Year: 2013..We recently showed that both acyl-CoA wax alcohol acyltransferases (AWAT) 1 and CGI-58 (a cofactor of triacylglycerol lipase) are further required for w-O-esterification, ..
- BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDESPei Zhou; Fiscal Year: 2013..They are: 1) mechanistic and structural studies of the acyltransferases LpxA and LpxD;2) the characterization of E...
- Regulation of Mitochondrial Dysfunction and Diet-Induced Obesity by ALCAT1Yuguang Shi; Fiscal Year: 2012..by a "remodeling" process that involves deacylation by phospholipases and reacylation by lysocardiolipin acyltransferases. Defective CL remodeling in response to reactive oxygen species (ROS) leads to accumulation of bad CL and ..
- Acyl-CoA Synthetase: Structure, Function and RegulationROSALIND ANNE COLEMAN; Fiscal Year: 2013..at least 50% of the protein is present on the endoplasmic reticulum where it interacts with glycerolipid acyltransferases;and 3) that the mechanism for these differences lies both in the membrane association and phosphorylation ..
- 2013 Molecular and Cellular Biology of Lipids Gordon Research ConferenceROSALIND ANNE COLEMAN; Fiscal Year: 2013..a broad range of topics, including Lipid signals that regulate cell metabolism, New roles for lipases and acyltransferases, Membrane biogenesis and turnover, Cellular lipid metabolism, Fatty acid metabolism, Transcriptional control ..
- Molecular Mechanism of Barth SyndromeMichael Schlame; Fiscal Year: 2009..The mutated gene (tafazzin) is homologous to a conserved family of phospholipid acyltransferases. Children with Barth syndrome are deficient in the mitochondria! phospholipid cardiolipin, suggesting that ..
- Glycine N-AcyltransferasesDavid J Merkler; Fiscal Year: 2013..We specifically propose to: (a) characterize a novel set of mammalian glycine N-acyltransferases (GLYATs) and define the role played by these enzymes in NAG biosynthesis and (b) determine the relative ..
- Global Characterization of Protein Palmitoylation in TrypanosomesDavid M Engman; Fiscal Year: 2013..rarely N-palmitoylation (occurring on an N-terminal cys residue), is mediated by one of a number of palmitoyl acyltransferases (PATs), each having its own localization and substrate specificity...
- EFFECTS OF POLYUNSATURATED FATTY ACIDS ON MEGAKARYOCYTESPAUL SCHICK; Fiscal Year: 1991..There is evidence desaturases and acyltransferases are sensitive to PUFA-modified diets...
- ENZYMES IN PHOSPHATIDIC ACID BIOSYNTHESISHei Sook Sul; Fiscal Year: 2002..in a unique position to address, by biochemical and molecular biological approaches, the role of these two acyltransferases in the positional distribution of fatty acids in glycerophospholipids and in the regulation of lipid ..
- SHORT-CHAIN ACYLCARNITINES--FUNCTION AND ENZYMOLOGYLORAN BIEBER; Fiscal Year: 1993..determination of partial amino acid sequences, and their properties will be compared to the other carnitine acyltransferases of liver...
- DIET AND CARCINOGENESIS BY PEROXISOME PROLIFERATORSHOWARD GLAUERT; Fiscal Year: 1993..of several proteins, including the enzymes of the peroxisomal beta-oxidation pathway, the carnitine acyltransferases, and cytochrome P-450IVA1...
- Aging and Lipid MetaboilismCHRISTOPHER JOLLY; Fiscal Year: 2001..The fatty acids have to be in the acyl-CoA form in order to be utilized by the acyltransferases. Kinetic studies will be conducted using oleoyl-CoA with or without albumin or ACBP present which increases ..
- STEROID DYNAMICS IN ENDOCRINOLOGYERLIO GURPIDE; Fiscal Year: 1991..and the effects of steroids, peptide hormones and calcium ionophore on the activities of phospholipases, acyltransferases, PG synthetase, and PG metabolizing enzymes...
- STRUCTURAL STUDIES OF A GROUP OF ACYLTRANSFERASESDonghai Wu; Fiscal Year: 2000..project is to understand the structural basis of substrate recognition and catalytic mechanism for a group of acyltransferases. Based on sequence homology and reaction mechanism, these acyltransferases constitute a unique gene family ..
- LIPID DEPOSITION IN MAMMALIAN CELLSARTHUR SPECTOR; Fiscal Year: 1980..enzymes involved in lipid metabolism such as NADPH-dependent lipid peroxidation, fatty acid desaturases, acyltransferases involved in phospholipid and triglyceride synthesis and HMGCoA reductase...
- HITACHI ELECTRON MICROSCOPEAlvin Beitz; Fiscal Year: 1990..Dr. Brady plans to use novel monoclonal antibodies against carnitine acyltransferases to analyze the location of these enzymes in the mitochondria and peroxisomes of liver cells.
- Lipid modification of secreted signaling proteinsJessica Treisman; Fiscal Year: 2009..Palmitoylation catalyzed by the acyltransferases Rasp and Porcupine promotes the secretion, transport and signaling activity of Hedgehog and Wnt family ..
- PROTEIN PALMITOYLATION IN SIGNALING SYSTEMSMaurine E Linder; Fiscal Year: 2010..The breakthrough in this field was our discovery of a large family of protein acyltransferases or PATs...
- Mutant Acyltransferases in Natural Product BiosynthesisANDREW KOPPISCH; Fiscal Year: 2003..This information is desirable for the rational design of efficiently biosynthesized unique polyketide metabolites. ..
- Inhibitors of Human Pal mitoyl AcyltransferaseCHARLES DUCKER; Fiscal Year: 2005The goal of this program is to develop novel inhibitors of human palmitoyl acyltransferases (PATs) that are effective as cancer therapeutic agents...
- Barth Syndrome: Identifying the missing enzyme functionTAL LEWIN; Fiscal Year: 2006..have mutations in the tafazzin gene (TAZ) which belongs to a family of established and putative glycerolipid acyltransferases. Consistent with this finding is that tissues from BTHS patients contain decreased cardiolipin (CL), a ..
- FASEB Summer Conference, July 20-25, 2002Fuyuhiko Tamanoi; Fiscal Year: 2002..thioacylation has been obtained, including the potential identification of the elusive first protein; fatty acyltransferases. Structural studies of the enzymes involved in protein prenylation and myristoylation have yield ..
- STRUCTURE AND FUNCTION OF HIV GAG PROTEINMARILYN RESH; Fiscal Year: 2009..Multicolor imaging with site-specific fluorescein derivatives (FLASH and ReAsH) will be used to determine the order in which Gag assembly sites are formed and maintained in the cell. ..
- Glial cell differentiation and glioma formationMARILYN RESH; Fiscal Year: 2006..The activity of the above proteins in human glioma tumor samples will be determined. ..
- The Role of DGAT1 In Muscle LipotoxicityYi Hao Yu; Fiscal Year: 2006..As a result, we may be able to identify new molecular target(s) for future drug therapy aimed at reducing lipotoxicity ..
- PURINE BIOSYNTHESIS ENZYMES-MECHANISMS AND REGULATIONJoAnne Stubbe; Fiscal Year: 2003..abstract_text> ..
- MECHANISM OF DRUG INDUCED DNA DEGRADATIONJoAnne Stubbe; Fiscal Year: 2005..The structures of these lesions will be determined. The repair of these lesions by Apel will be investigated in an effort to determine its relevance to the non-homologous end joining ds-break repair. ..
- Polyhydroxyalkanoates: A paradigm for non-template dependent polymerizationsJoAnne Stubbe; Fiscal Year: 2009..Understanding the roles of PhaC, PhaP and PhaZIa would be greatly enhanced with structural insight and efforts to crystallize proteins involved in PHB homeostasis will be actively pursued. ..
- DGAT Overexpression In Mouse Adipose Tissue and MuscleYi Hao Yu; Fiscal Year: 2006....
- Amine N-Oxygenation by FMO3 and FMO4John Cashman; Fiscal Year: 2005..Such fundamental information will be useful in the development of safer drugs, the prevention of adverse drug reactions and the protection of humans from disease. ..
- MOLECULAR MECHANISMS IN ENDOTHELIAL DYSFUNCTION IN HIV 1John Ho; Fiscal Year: 2004....
- THE PATHOGENESIS OF AUTOIMMUNITY IN A MURINE MODEL OF PRIMARY BILIARY CIRRHOSISMERRILL E GERSHWIN; Fiscal Year: 2010..abstract_text> ..
- MECHANISM & STRUCTURE OF HUMAN GAR TRANSFORMYLASECAROL CAPERELLI; Fiscal Year: 2004..Although it has been demonstrated that inhibition of this pathway shows potential for cancer chemotherapy, much basic information about the pathway and its constituent enzymes remains to be elucidated. ..
- Health and QOL among Long-Term Lung Cancer SurvivorsPing Yang; Fiscal Year: 2010....
- Vitamin A Storage and MetabolismWilliam S Blaner; Fiscal Year: 2010..Finally, in Aim 3 we will investigate how vitamin A is mobilized from HSCs and whether this requires direct involvement of serum retinol-binding protein (RBP) synthesized in hepatocytes. ..
- Inflammatory Biomarkers and Colorectal Cancer RiskGong Yang; Fiscal Year: 2010..Overall, this study will contribute significantly to the understanding of the role of inflammation in the etiology of CRC and to the development of new strategies for the assessment of CRC risk. ..
- Novel Therapies for Metabolic Complications in Patients with LipodystrophiesAbhimanyu Garg; Fiscal Year: 2010..Additionally, the findings may be applicable to treating metabolic complications in patients with obesity and type 2 diabetes. ..
- Lipid Metabolism in the Aging Immune SystemCHRISTOPHER JOLLY; Fiscal Year: 2006..abstract_text> ..
- Conference on Drug Against Tropical Protozoan ParasitesMichael Gelb; Fiscal Year: 2002..We consider the poster sessions that occur throughout our meeting to be just as important as the collection of lecture presentations. ..
- MODULATION OF DNA REPAIR BY P53 IN HUMAN UROEPITHELIUMSanthanam Swaminathan; Fiscal Year: 2001..The results of these studies have important etiologic, mechanistic and therapeutic implications in human cancers. ..
- STRUCTURAL STUDIES ON RIBOSOMAL COMPONENTSStephen White; Fiscal Year: 2002..Future work will improve this image to accurately orient the IF3 molecule, and additional factors will be added to build up the complete initiation complex. ..
- 9.4 Tesla Fourier Transform Mass SpectrometerStephen Barnes; Fiscal Year: 2002..Overall, the FT-MS instrument will sustain the high quality of biomedical research that is a hallmark of UAB. ..
- ACYLTRANSFERASE INHIBITORS AS ANTI RAS AGENTSCharles Smith; Fiscal Year: 2009The overall goal of this program is to develop novel inhibitors of human protein acyltransferases (hPATs) that =re effective as cancer therapeutic agents...
- Development of DHPS as a Bioterrorism Therapeutic TargetStephen White; Fiscal Year: 2005..The institutions have complementary programs in infectious diseases with a strong emphasis on studying bioterrorism. ..
- RNA Interference using gutless adenoviral vectorsNuria Morral; Fiscal Year: 2005..Upon completion of our studies, the system could be used in a large number of research applications directed at studying gene function in this organ as well as many other tissues that can be transduced by adenoviral vectors. ..
- SPHINGOMYELIN TURNOVER AND CELLULAR REGULATIONYusuf Hannun; Fiscal Year: 2006..These pathways are of direct significance to the biology of stress responses and specifically to cancer therapeutics. ..
- Ceramide in angiogenic integrin signalingAnat Erdreich Epstein; Fiscal Year: 2006....
- Thiol-Reactive Crosslinkers for BiomaterialsGlenn Prestwich; Fiscal Year: 2004..Fourth, these new materials will be evaluated for cytocompatibility and cell growth in vitro with murine fibroblasts. Finally, selected materials will be seeded with fibroblasts and injected into nude mice for in vivo tissue generation. ..
- Therapeutic Approaches to HAART-Induced LipodystrophyAbhimanyu Garg; Fiscal Year: 2006..Additionally, the study may help prevent these problems in HIV-infected patients being initiated on PI-containing HAART. ..
- RPE65 IN RETINAL METABOLISM AND DEGENERATIONDebra Thompson; Fiscal Year: 2005..The long-term goals of this project are to elucidate the mechanisms by which RPE65 defects contribute to retinal degeneration, and to lay the groundwork for the development of therapeutic approaches to the disease. ..
- Carotene Cleavage Enzyme: Properties and PhysiologyWILLIAM BLANER; Fiscal Year: 2007..In Aim 4, we propose to use a cDNA clone for human CCE to characterize the biochemical properties of the recombinant enzyme. ..
- Retinyl Ester Binding Proteins and the Visual CycleRobert Rando; Fiscal Year: 2007..unreadable] [unreadable]..
- MBD2 as a Target for Cancer Prevention and TreatmentWilliam Nelson; Fiscal Year: 2007..abstract_text> ..
- REGENERATION OF 11-CIS-RETINAL IN THE RETINARobert Rando; Fiscal Year: 2007..Finally, we plan to synthesize novel antagonists of IMH to aid in understanding its physiological role(s). Specific IMH antagonists will also be used to limit the visual cycle in approaches to the treatment of macular degeneration. ..
- Chemopreventive Actions of Equol EnantiomersKenneth Setchell; Fiscal Year: 2007..These studies are relevant to humans given the ammended health claim for soy and cancer risk-reduction currently being reviewed by the FDA. ..
- The Glucoregulatory and Liporegulatory Peptide HormonesRoger Unger; Fiscal Year: 2008..Using FA-depleted islets we have been able to "cure" insulin-deficient diabetes in rats for ~1 year without immunosuppression. [unreadable] [unreadable] [unreadable]..
- Brain tumor angiogenesis: Integrin ceramide signalingAnat Erdreich Epstein; Fiscal Year: 2008..abstract_text> ..
- Radioimmunotherapy of CD20+ Lymphomas & CD45+LeukemiasJOHN PAGEL; Fiscal Year: 2008..abstract_text> ..
- Role of DAP12 in type 1 anti-mycobacterial immunityZhou Xing; Fiscal Year: 2005..We strongly believe that our studies will provide new insights into the mechanisms of type 1 anti-microbial immunity and novel targets for immune modulation. ..