rec a recombinases

Summary

Summary: A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.

Top Publications

  1. ncbi DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression
    D K Bishop
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 69:439-56. 1992
  2. ncbi Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
    A Shinohara
    Department of Biology, Faculty of Science, Osaka University, Japan
    Cell 69:457-70. 1992
  3. ncbi Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
    Zhucheng Chen
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 453:489-4. 2008
  4. ncbi The bacterial LexA transcriptional repressor
    M Butala
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Cell Mol Life Sci 66:82-93. 2009
  5. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
  6. pmc Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Microbiol Mol Biol Rev 63:349-404. 1999
  7. pmc Purified lexA protein is a repressor of the recA and lexA genes
    J W Little
    Proc Natl Acad Sci U S A 78:4199-203. 1981
  8. pmc SSB protein diffusion on single-stranded DNA stimulates RecA filament formation
    Rahul Roy
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nature 461:1092-7. 2009
  9. pmc PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps
    Jeehae Park
    Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Cell 142:544-55. 2010
  10. pmc Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins
    Tribhuwan Yadav
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, 28049 Madrid, Spain
    Nucleic Acids Res 40:5546-59. 2012

Research Grants

  1. UNDERSTANDING HOMOLOGY IN GENETIC RECOMBINATION
    Scott F Singleton; Fiscal Year: 2012
  2. THE BIOCHEMISTRY OF GENETIC RECOMBINATION/RECA PROTEIN
    Michael M Cox; Fiscal Year: 2013
  3. SSB PROTEIN/DNA INTERACTIONS
    Timothy M Lohman; Fiscal Year: 2013
  4. Biochemical Mechanisms and Function of DNA Helicases
    STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2013
  5. NEW GENE THERAPY FOR CONNECTIVE TISSUE DISEASES
    Eric Kmiec; Fiscal Year: 1999
  6. DNA DAMAGE, REPAIR, AND MUTAGENESIS
    IRWIN TESSMAN; Fiscal Year: 1992
  7. DNA STRUCTURE AND RECOMBINATIONAL INTERMEDIATES
    Brian Johnston; Fiscal Year: 1993
  8. MECHANISM OF PROTEIN SPLICING IN MYCOBACTERIUM
    Henry Paulus; Fiscal Year: 2000
  9. RECOMBINATION IN VITRO: ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 1993
  10. RECOMBINATION IN VITRO--ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 2003

Detail Information

Publications261 found, 100 shown here

  1. ncbi DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression
    D K Bishop
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 69:439-56. 1992
    ..DMC1 is homologous to yeast's RAD51 gene, supporting the view that mitotic DSB repair has been recruited for use in meiotic chromosome metabolism...
  2. ncbi Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
    A Shinohara
    Department of Biology, Faculty of Science, Osaka University, Japan
    Cell 69:457-70. 1992
    ..Rad51 protein is also homologous to a meiosis-specific Dmc1 protein of S. cerevisiae...
  3. ncbi Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
    Zhucheng Chen
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 453:489-4. 2008
    ..The underwound, stretched filament conformation probably evolved to destabilize the donor duplex, freeing the complementary strand for homology sampling...
  4. ncbi The bacterial LexA transcriptional repressor
    M Butala
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Cell Mol Life Sci 66:82-93. 2009
    ..These may provide new starting points in the battle against the emergence of bacterial pathogens and the spread of drug resistance among them...
  5. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
    ..Thus, we present a mechanism by which therapeutic agents can promote the spread of antibiotic resistance genes...
  6. pmc Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Microbiol Mol Biol Rev 63:349-404. 1999
    ..This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination...
  7. pmc Purified lexA protein is a repressor of the recA and lexA genes
    J W Little
    Proc Natl Acad Sci U S A 78:4199-203. 1981
    ..Finally, we found that purified lexA protein was a substrate for the recA protease in a reaction requiring ATP or an analogue, adenosine 5'-[gamma-thio]triphosphate, and denatured DNA...
  8. pmc SSB protein diffusion on single-stranded DNA stimulates RecA filament formation
    Rahul Roy
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nature 461:1092-7. 2009
    ..This observation of diffusional movement of a protein on ssDNA introduces a new model for how an SSB protein can be redistributed, while remaining tightly bound to ssDNA during recombination and repair processes...
  9. pmc PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps
    Jeehae Park
    Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Cell 142:544-55. 2010
    ....
  10. pmc Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins
    Tribhuwan Yadav
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, 28049 Madrid, Spain
    Nucleic Acids Res 40:5546-59. 2012
    ..Our results provide a mechanistic framework for rationalizing the coordinated events modulated by SsbA, SsbB and RecO that are crucial for RecA-dependent chromosomal transformation and RecA-independent plasmid transformation...
  11. pmc Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA
    Jason C Bell
    Department of Microbiology, University of California, Davis, California 95616, USA
    Nature 491:274-8. 2012
    ..We further demonstrate that the recombination mediator protein pair, RecOR (RecO and RecR), accelerates both RecA nucleation and filament growth, and that the introduction of RecF further stimulates RecA nucleation...
  12. ncbi Aeons of distress: an evolutionary perspective on the bacterial SOS response
    Ivan Erill
    Biomedical Applications Group, Centro Nacional de Microelectronica, Barcelona, Spain
    FEMS Microbiol Rev 31:637-56. 2007
    ....
  13. ncbi Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway
    A Schwacha
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 90:1123-35. 1997
    ..Coordinate action of mitotic recA homologs as one functional unit, two functions of RED1, and an interhomolog interaction function of DMC1 are also revealed...
  14. ncbi A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA
    Isabelle Mortier-Barrière
    Laboratoire de Microbiologie et Genetique Moleculaires, UMR 5100 CNRS Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 09, France
    Cell 130:824-36. 2007
    ..We propose that DprA is a new member of the recombination-mediator protein family, dedicated to natural bacterial transformation...
  15. pmc Single-molecule imaging of DNA pairing by RecA reveals a three-dimensional homology search
    Anthony L Forget
    Department of Microbiology, University of California, Davis, California 95616 8665, USA
    Nature 482:423-7. 2012
    ....
  16. ncbi RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis
    D K Bishop
    Department of Radiation and Cellular Oncology, University of Chicago, Illinois 60638
    Cell 79:1081-92. 1994
    ..Analysis of zip1 mutants shows that Zip1 promotes dissociation of Dmc1 complexes. Colocalization of Dmc1 and Zip1 raises the possibility that Dmc1 and Rad51 are components of recombination nodules...
  17. ncbi Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination
    A Shinohara
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Japan
    Genes Cells 2:615-29. 1997
    ..Mutant analysis has shown that both proteins are required for normal meiotic recombination, for timely and efficient formation of synaptonemal complex and for normal progression out from meiotic prophase...
  18. pmc A major role of the RecFOR pathway in DNA double-strand-break repair through ESDSA in Deinococcus radiodurans
    Esma Bentchikou
    Universite Paris Sud 11, CNRS UMR 8621, LRC CEA 42V, Institut de Genetique et Microbiologie, Orsay, France
    PLoS Genet 6:e1000774. 2010
    ....
  19. pmc Inducible SOS response system of DNA repair and mutagenesis in Escherichia coli
    Celina Janion
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Int J Biol Sci 4:338-44. 2008
    ..but are not organized in an SOS system. This paper presents a brief up-to-date review describing the discovery of the SOS system, the physiology of SOS induction, methods for its determination, and the role of some SOS-induced genes...
  20. ncbi The RecA protein: structure and function
    A I Roca
    Department of Biochemistry, University of Wisconsin Madison 53706
    Crit Rev Biochem Mol Biol 25:415-56. 1990
  21. pmc The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP
    Qingfei Jiang
    Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California 90089 2910, USA
    Nature 460:359-63. 2009
    ..Reactivation of pol V Mut is triggered by replacement of RecA-ATP from RecA*. Thus, the principal role of RecA* in SOS mutagenesis is to transfer RecA-ATP to pol V, and thus generate active mutasomal complex for translesion synthesis...
  22. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. I. Bypassing a short heterologous insert in one DNA substrate
    J I Kim
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 267:16438-43. 1992
    ....
  23. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. III. Unidirectional branch migration and extensive hybrid DNA formation
    S K Jain
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 269:20653-61. 1994
    ..Both of these functions of RecA protein-mediated ATP hydrolysis are crucial in ensuring the effectiveness of recombinational DNA repair, especially when the lesion to be repaired is distant from the initial crossover point...
  24. ncbi The majority of inducible DNA repair genes in Mycobacterium tuberculosis are induced independently of RecA
    Lucinda Rand
    National Institute for Biomedical Research, London, UK
    Mol Microbiol 50:1031-42. 2003
    ..Amongst these are genes involved in nucleotide excision repair, base excision repair, damage reversal and recombination. Thus, it appears that this novel mechanism of gene regulation is important for DNA repair in M. tuberculosis...
  25. pmc The RecU Holliday junction resolvase acts at early stages of homologous recombination
    Cristina Cañas
    Departmento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, C Darwin 3, Campus Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Nucleic Acids Res 36:5242-9. 2008
    ..Both activities are important in vivo since RecU mutants impaired only in RecA interaction are as sensitive to DNA damaging agents as a deletion mutant...
  26. ncbi From meiosis to postmeiotic events: uncovering the molecular roles of the meiosis-specific recombinase Dmc1
    Wataru Kagawa
    Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku ku, Tokyo, Japan
    FEBS J 277:590-8. 2010
    ..Recent structural, biochemical, and genetic findings are now revealing the molecular mechanisms of Dmc1-mediated homologous recombination and its regulation by various recombination mediators...
  27. pmc ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis
    Eugenio Sanchez-Moran
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Genes Dev 21:2220-33. 2007
    ..Thus, ASY1 plays a key role in coordinating the activity of the RecA homologs to create a bias in favor of interhomolog recombination...
  28. ncbi Double-strand break repair in bacteria: a view from Bacillus subtilis
    Silvia Ayora
    Departmento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Cantoblanco, Madrid, Spain
    FEMS Microbiol Rev 35:1055-81. 2011
    ..In this review, we examine the functions that are known to contribute to DNA DSB repair in B. subtilis, and compare their properties with those of other bacterial phyla...
  29. ncbi Regulation of bacterial RecA protein function
    Michael M Cox
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Crit Rev Biochem Mol Biol 42:41-63. 2007
    ..Additional regulatory proteins may remain to be discovered. The elaborate regulatory pattern is likely to be reprised for RecA homologues in archaeans and eukaryotes...
  30. ncbi PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
    ....
  31. pmc RecA-independent pathways of lambdoid prophage induction in Escherichia coli
    D V Rozanov
    State Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia
    J Bacteriol 180:6306-15. 1998
    ....
  32. ncbi RecA acts in trans to allow replication of damaged DNA by DNA polymerase V
    Katharina Schlacher
    Department of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, California 90089 2910, USA
    Nature 442:883-7. 2006
    ....
  33. pmc Mechanism of action of the lexA gene product
    R Brent
    Proc Natl Acad Sci U S A 78:4204-8. 1981
    ..Differences in the affinity with which lexA protein binds to the operators of genes it represses may account for the differences in the timing and extent of their induction after DNA damage...
  34. pmc RecFOR proteins target RecA protein to a DNA gap with either DNA or RNA at the 5' terminus: implication for repair of stalled replication forks
    Katsumi Morimatsu
    Department of Microbiology and of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    J Biol Chem 287:35621-30. 2012
    ..RecFOR proteins also recognize RNA at the 5'-end of an RNA-DNA junction within an ssDNA gap, which is compatible with their role in the repair of lagging strand gaps at stalled replication forks...
  35. pmc Rad51 polymerization reveals a new chromatin remodeling mechanism
    Pauline Dupaigne
    CNRS, Laboratoire de Microscopie Moléculaire et Cellulaire, UMR 8126 Interactions Moléculaires et Cancer, Institut de Cancerologie Gustave Roussy, Villejuif, France
    PLoS ONE 3:e3643. 2008
    ..Thus, recombinase polymerization is a powerful mechanism of chromatin remodeling. These remarkable features open up new possibilities for understanding DNA recombination and reveal new types of ATP-dependent chromatin dynamics...
  36. ncbi Together yes, but not coupled: new insights into the roles of RAD51 and DMC1 in plant meiotic recombination
    Mónica Pradillo
    Departamento de Genetica, Facultad de Biologia, Universidad Complutense de Madrid, Madrid 28040, Spain
    Plant J 69:921-33. 2012
    ....
  37. pmc Developing single-molecule TPM experiments for direct observation of successful RecA-mediated strand exchange reaction
    Hsiu Fang Fan
    Department of Chemistry, National Taiwan University, Taipei, Taiwan
    PLoS ONE 6:e21359. 2011
    ..In addition, the Brownian motion time-courses suggest that the strand exchange process progresses uni-directionally in the 5'-to-3' fashion, using a synapse segment with a wide and continuous size distribution...
  38. ncbi The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction
    B Martin
    Microbiologie et Génétique Moléculaire CNRS UPR, Universite Paul Sabatier, Toulouse, France
    Mol Microbiol 15:367-79. 1995
    ..This is unlike the situation of a recA+ lysogen. Together these results demonstrate that RecA controls lysogenic induction and suggest the existence of a SOS repair system in S. pneumoniae...
  39. pmc The RecA proteins of Deinococcus radiodurans and Escherichia coli promote DNA strand exchange via inverse pathways
    Jong Il Kim
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 99:7917-21. 2002
    ..This reaction lies at the heart of a remarkably efficient system for the repair of DNA damage...
  40. ncbi The mutagenesis protein UmuC is a DNA polymerase activated by UmuD', RecA, and SSB and is specialized for translesion replication
    N B Reuven
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 274:31763-6. 1999
    ..UmuC is a member of a new family of DNA polymerases which are specialized for lesion bypass, and include the yeast RAD30 and the human XP-V genes, encoding DNA polymerase eta...
  41. pmc Real-time observation of strand exchange reaction with high spatiotemporal resolution
    Kaushik Ragunathan
    Center for Biophysics and Computational Biology, Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Structure 19:1064-73. 2011
    ..This finding could have important implications for the irreversibility of strand exchange. Our model for strand exchange links structural models of RecA to its catalytic function...
  42. pmc The interplay of RecA-related proteins and the MND1-HOP2 complex during meiosis in Arabidopsis thaliana
    Julien Vignard
    Station de Genetique et d Amelioration des plantes, INRA, Versailles, France
    PLoS Genet 3:1894-906. 2007
    ..Furthermore, they highlight a direct interaction between MND1 and RAD51 and show a functional divergence between RAD51 and XRCC3...
  43. ncbi The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis
    K Yoshida
    Division of Molecular Embryology, Osaka University, Japan
    Mol Cell 1:707-18. 1998
    ..In rare cases, however, we observed complex pairing among nonhomologs. Thus, the mouse Dmc1 gene is required for homologous synapsis of chromosomes in meiosis...
  44. pmc Biochemistry of homologous recombination in Escherichia coli
    S C Kowalczykowski
    Division of Biological Sciences, University of California, Davis 95616 8665
    Microbiol Rev 58:401-65. 1994
    ..This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination...
  45. pmc Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli
    Sunny Park
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 102:9317-22. 2005
    ..The rate constant of the Fenton reaction measured at physiological pH was much higher than under the acidic conditions that were used to determine the commonly cited value...
  46. ncbi Localization of RecA in Escherichia coli K-12 using RecA-GFP
    Nicholas Renzette
    Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA
    Mol Microbiol 57:1074-85. 2005
    ..This suggested colocalization with the DNA replication factory. recA, recB and recF strains showed phenotypes and distributions of foci consistent with the predicted effects of these mutations...
  47. ncbi Ringlike structure of the Deinococcus radiodurans genome: a key to radioresistance?
    Smadar Levin-Zaidman
    Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Science 299:254-6. 2003
    ....
  48. ncbi DNA strand exchange promoted by RecA K72R. Two reaction phases with different Mg2+ requirements
    Q Shan
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 271:5712-24. 1996
    ..The mutant protein also facilitates the autocatalytic cleavage of the LexA repressor, but at a reduced rate...
  49. pmc ClpR protein-like regulator specifically recognizes RecA protein-independent promoter motif and broadly regulates expression of DNA damage-inducible genes in mycobacteria
    Yi Wang
    National Key Laboratory of Agricultural Microbiology, Center for Proteomics Research, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
    J Biol Chem 286:31159-67. 2011
    ..These results enhance our understanding of the function of the ClpR regulator and the regulatory mechanism of RecA-independent DNA repair in mycobacteria...
  50. ncbi Role of recA/RAD51 family proteins in mammals
    Masahiro Kawabata
    Department of Pharmacology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Acta Med Okayama 59:1-9. 2005
    ..In addition, identification of alternative transcripts of some RAD51 paralogs may reflect the complexity of the homologous recombination system...
  51. ncbi Function and regulation of the cyanobacterial genes lexA, recA and ruvB: LexA is critical to the survival of cells facing inorganic carbon starvation
    Francis Domain
    Service de Biochimie et Génétique Moléculaire, 91191 Gif sur Yvette Cedex, France
    Mol Microbiol 53:65-80. 2004
    ..Consistently, the growth of the LexA-depleted strain was found to be strongly dependent on the availability of inorganic carbon...
  52. pmc Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins
    Paula P Cardenas
    Centro Nacional de Biotecnologia CSIC, Darwin 3, 28049 Madrid, Spain
    Nucleic Acids Res 39:9250-61. 2011
    ..subtilis PNPase might also contribute to repair via NHEJ...
  53. ncbi Long CTG.CAG repeats from myotonic dystrophy are preferred sites for intermolecular recombination
    Anna Pluciennik
    Institute of Biosciences and Technology, Center for Genome Research, Texas A and M University System Health Science Center, Texas Medical Center, 2121 W Holcombe Boulevard, Houston, TX 77030, USA
    J Biol Chem 277:34074-86. 2002
    ..We propose that DNA polymerases stall within the CTG.CAG repeat tracts causing nicks or double-strand breaks that stimulate homologous recombination. The recombination process is RecA-dependent...
  54. ncbi Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences
    B Krawczyk
    Department of Microbiology, Technical University of Gdansk, Poland
    Mol Cell Probes 16:1-11. 2002
    ..In this context, recA/RFLP genotypic method should be seen as an ideal preliminary screening method for large numbers of isolates, with the ultimate confirmatory role reserved for DNA hybridization analysis...
  55. ncbi BRCA2 is a mediator of RAD51- and DMC1-facilitated homologous recombination in Arabidopsis thaliana
    Katharina Seeliger
    Botanical Institute II, Karlsruhe Institute of Technology KIT, Karlsruhe, Germany
    New Phytol 193:364-75. 2012
    ..We further show that AtBRCA2 is required for proper meiotic synapsis and mediates the recruitment of AtRAD51 and AtDMC1. Our results suggest that BRCA2 controls single-strand invasion steps during homologous recombination in plants...
  56. pmc Complementary strand relocation may play vital roles in RecA-based homology recognition
    Alexandra Peacock-Villada
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Nucleic Acids Res 40:10441-51. 2012
    ..This differential extension drives strand exchange forward for homologs and increases the free energy penalty for strand exchange of non-homologs...
  57. ncbi RecA-promoted, RecFOR-independent progressive disassembly of replisomes stalled by helicase inactivation
    Giuseppe Lia
    CNRS, Centre de Genetique Moleculaire, UPR3404, Gif sur Yvette 91198, France
    Mol Cell 49:547-57. 2013
    ..Our results identify a function for the universally conserved, central recombination protein RecA...
  58. ncbi Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae
    I Mortier-Barriere
    Microbiologie et Génétique Moléculaire CNRS UPR 9007, Universite Paul Sabatier, Toulouse, France
    Mol Microbiol 27:159-70. 1998
    ..The role of recA induction is discussed in view of the potential contribution of transformation to genome plasticity in this pathogen...
  59. pmc DNA double-strand breaks caused by replication arrest
    B Michel
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    EMBO J 16:430-8. 1997
    ..This reveals the existence of a significant amount of spontaneous DSBs. We propose that some of them may also result from the impairment of replication fork progression...
  60. pmc Interconversion between bound and free conformations of LexA orchestrates the bacterial SOS response
    Matej Butala
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    Nucleic Acids Res 39:6546-57. 2011
    ..We show that, while LexA exhibits diverse dissociation rates from operators, it interacts extremely rapidly with DNA target sites. Modulation of LexA activity changes the occurrence of persister cells in bacterial populations...
  61. pmc MCM8 is required for a pathway of meiotic double-strand break repair independent of DMC1 in Arabidopsis thaliana
    Wayne Crismani
    INRA, UMR1318, Institut Jean Pierre Bourgin, RD10, Versailles, France
    PLoS Genet 9:e1003165. 2013
    ..We propose that MCM8 is involved with RAD51 in a backup pathway that repairs meiotic DSB without giving CO when the major pathway, which relies on DMC1, fails...
  62. pmc RecFOR and RecOR as distinct RecA loading pathways
    Akiko Sakai
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706 1544, USA
    J Biol Chem 284:3264-72. 2009
    ..The RecOR pathway is more efficient for RecA loading onto ssDNA when no proximal dsDNA is available. A merger of new and published results leads to a new model for RecFOR function...
  63. pmc A new model for SOS-induced mutagenesis: how RecA protein activates DNA polymerase V
    Meghna Patel
    Departments of Biological Sciences and Chemistry, University of Southern California, Los Angeles, CA, USA
    Crit Rev Biochem Mol Biol 45:171-84. 2010
    ..Based on unforeseen properties of RecA*, we describe a new model for pol V-catalyzed SOS-induced mutagenesis...
  64. pmc Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks
    Nicolas C Stephanou
    Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Bacteriol 189:5237-46. 2007
    ..These findings demonstrate that prokaryotic NHEJ is specifically required for DSB repair in late stationary phase and can mediate mutagenic repair of homing endonuclease-generated chromosomal DSBs...
  65. pmc The rec locus, a competence-induced operon in Streptococcus pneumoniae
    B J Pearce
    Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021
    J Bacteriol 177:86-93. 1995
    ..Therefore, the rec locus is directly required for genetic transformation and is regulated by the cell signaling mechanism that induces competence...
  66. ncbi Homologous recombination in real time: DNA strand exchange by RecA
    Thijn van der Heijden
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Mol Cell 30:530-8. 2008
    ....
  67. pmc Differing requirements for RAD51 and DMC1 in meiotic pairing of centromeres and chromosome arms in Arabidopsis thaliana
    Olivier Da Ines
    Genetique, Reproduction et Développement, UMR CNRS 6293, Clermont Universite, INSERM U1103, Aubiere, France
    PLoS Genet 8:e1002636. 2012
    ....
  68. ncbi SOS regulatory elements are essential for UPEC pathogenesis
    Birong Li
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, The Ohio State University School of Medicine, Columbus, OH 43205, USA
    Microbes Infect 12:662-8. 2010
    ....
  69. pmc Caught in the act: the lifetime of synaptic intermediates during the search for homology on DNA
    Adam Mani
    Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel
    Nucleic Acids Res 38:2036-43. 2010
    ..These results suggest the need for facilitated homology search mechanisms to locate homology successfully within the timescales observed in vivo...
  70. pmc RecA polymerization on double-stranded DNA by using single-molecule manipulation: the role of ATP hydrolysis
    G V Shivashankar
    Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 96:7916-21. 1999
    ..Part of this dynamics is suppressed in the presence of ATP[gammaS], leading to a stabilized RecA-DNA complex. A one-dimensional nucleation and growth model is presented that may account for the protein assembly...
  71. pmc The mycobacterium-specific gene Rv2719c is DNA damage inducible independently of RecA
    Patricia C Brooks
    Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW71AA, United Kingdom
    J Bacteriol 188:6034-8. 2006
    ....
  72. ncbi Mycobacterium tuberculosis UvrD1 and UvrA proteins suppress DNA strand exchange promoted by cognate and noncognate RecA proteins
    Pawan Singh
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
    Biochemistry 49:4872-83. 2010
    ..These observations are consistent with a molecular mechanism, whereby M. tuberculosis UvrA and UvrD1, acting together, block DNA strand exchange promoted by cognate and noncognate RecA proteins...
  73. pmc Homologous recombination in procaryotes
    G R Smith
    Microbiol Rev 52:1-28. 1988
  74. ncbi DNA damage induction of recA in Mycobacterium tuberculosis independently of RecA and LexA
    Elaine O Davis
    Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Mol Microbiol 46:791-800. 2002
    ..These observations demonstrate convincingly for the first time that there is a novel mechanism of DNA damage induction in M. tuberculosis that is independent of LexA and RecA...
  75. pmc RecA homology search is promoted by mechanical stress along the scanned duplex DNA
    Claudia Danilowicz
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Nucleic Acids Res 40:1717-27. 2012
    ..The stress resulting from different levels of tension on its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present...
  76. ncbi The stalk region of the RecU resolvase is essential for Holliday junction recognition and distortion
    Cristina Cañas
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Darwin 3, Campus Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Mol Biol 410:39-49. 2011
    ..Our biochemical study provides a mechanistic basis for the connections between these two mutually exclusive stages (i.e., RecA modulation and HJ resolution) of the recombination reaction...
  77. pmc Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs
    L Worth
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
    Proc Natl Acad Sci U S A 91:3238-41. 1994
    ..Analysis of strand-transfer intermediates that accumulate in the presence of MutS and MutL indicates that the proteins block branch migration, presumably in response to occurrence of mispairs within newly formed heteroduplex...
  78. pmc Plant mitochondrial recombination surveillance requires unusual RecA and MutS homologs
    Vikas Shedge
    Plant Science Initiative, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Plant Cell 19:1251-64. 2007
    ....
  79. ncbi rec genes and homologous recombination proteins in Escherichia coli
    A J Clark
    Department of Molecular and Cell Biology, Barker GPBB ASU, University of California, Berkeley 94720
    Biochimie 73:523-32. 1991
    ..Specific proposals are made for which recombination genes might encode such products. Finally the generality of the RecA-ssDNA-filament mechanism of synapsis in the cellular biological world is discussed...
  80. ncbi Cloning and characterization of the recA genes from Mycoplasma pulmonis and M. mycoides subsp. mycoides
    K W King
    Department of Microbiology, University of Alabama at Birmingham 35294
    Gene 139:111-5. 1994
    ..The recA genes of Mm and Mp would encode proteins of 345 amino acids (aa) and 339 aa, respectively. The mycoplasmal RecA proteins revealed strong conservation when compared with RecA sequences from other bacterial species...
  81. ncbi A postsynaptic role for single-stranded DNA-binding protein in recA protein-promoted DNA strand exchange
    P E Lavery
    Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611
    J Biol Chem 267:9315-20. 1992
    ....
  82. pmc Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA
    Marielle C Gruenig
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 285:37188-97. 2010
    ..Thus, in N. gonorrhoeae, recombinational processes are facilitated by RecX(Ng) protein-mediated limitations on RecA(Ng) filament presence and/or length to achieve maximal function...
  83. pmc Structure of the LexA-DNA complex and implications for SOS box measurement
    Adrianna P P Zhang
    Department of Chemistry, The University of Chicago, Chicago, Illinois 60637, USA
    Nature 466:883-6. 2010
    ..However, the wings of these two DNA-binding domains bind to the same minor groove of the DNA. These wing-wing contacts may explain why the spacing between the two half-sites of E. coli SOS boxes is invariant...
  84. ncbi Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands
    Mathieu Berge
    Laboratoire de Microbiologie et Genetique Moleculaires, UMR 5100 CNRS Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France
    Mol Microbiol 50:527-36. 2003
    ..Our results demonstrate that incoming ssDNA requires active protection prior to the RecA-driven search for homology and that both DprA and RecA are needed for this protection...
  85. ncbi Recombination proteins and rescue of arrested replication forks
    Bénédicte Michel
    Centre de Genetique Moleculaire, Batiment 26, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    DNA Repair (Amst) 6:967-80. 2007
    ....
  86. pmc Dynamics of RecA filaments on single-stranded DNA
    Marijn T J van Loenhout
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nucleic Acids Res 37:4089-99. 2009
    ..Taken together, our results demonstrate that ATP hydrolysis has a major influence on the structure and state of RecA filaments on ssDNA...
  87. pmc RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis
    H Shinagawa
    Department of Experimental Chemotherapy, Osaka University, Japan
    Proc Natl Acad Sci U S A 85:1806-10. 1988
    ..The results suggested that UmuD protein may be proteolytically processed by RecA, and that processed UmuD may be the active form of the protein participating in mutagenesis...
  88. pmc Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA
    F E Benson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 13:5764-71. 1994
    ..In contrast to yeast Rad51 protein, human Rad51 forms filaments with single-stranded DNA in the presence of ATP/ATP gamma S. These resemble the inactive form of the RecA filament which is observed in the absence of a nucleotide cofactor...
  89. ncbi Roles for two RecA homologs in promoting meiotic chromosome synapsis
    B Rockmill
    Department of Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Genes Dev 9:2684-95. 1995
    ..In a zip1 haploid, axial associations are not apparent, suggesting that these associations depend on interactions between homologous sequences...
  90. ncbi Direct evaluation of a kinetic model for RecA-mediated DNA-strand exchange: the importance of nucleic acid dynamics and entropy during homologous genetic recombination
    Jie Xiao
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251 1892, USA
    Chembiochem 7:1265-78. 2006
    ..The data best support the conclusion that the mechanistic strategy of RecA likely relies on intrinsic DNA dynamics...
  91. ncbi Error-free recombinational repair predominates over mutagenic translesion replication in E. coli
    Ala Berdichevsky
    Department of Biological Chemistry, The Weizmann Institute of Science, 76100, Rehovot, Israel
    Mol Cell 10:917-24. 2002
    ..This molecular hierarchy in the tolerance of DNA lesions ensures that the nonmutagenic RR predominates over the mutagenic TLR, thereby contributing to genetic stability...
  92. ncbi No sliding during homology search by RecA protein
    K Adzuma
    The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:31565-73. 1998
    ..These and other available data can be adequately explained by a simple three-dimensional random collision mechanism...
  93. ncbi The recX gene potentiates homologous recombination in Neisseria gonorrhoeae
    E A Stohl
    Department of Microbiology-Immunology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Mol Microbiol 40:1301-10. 2001
    ..Although the precise role of recX in recombination remains unclear, RecX aids all RecA-related processes in Gc, and this is the first demonstration of a role for recX in homologous recombination in any organism...
  94. pmc Running in reverse: the structural basis for translocation polarity in hexameric helicases
    Nathan D Thomsen
    Department of Molecular and Cell Biology, Quantitative Biosciences Institute, University of California, Berkeley, CA 94720, USA
    Cell 139:523-34. 2009
    ..For a video summary of this article, see the PaperFlick file with the Supplemental Data available online...
  95. pmc New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis
    M Dutreix
    Groupe d Etude Mutagénèse et Cancérogénèse, Enzymologie, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Bacteriol 171:2415-23. 1989
    ..With respect to other RecA functions, recA1730 was recessive to recA+. This demonstrates that RecA protein has an additional role in mutagenesis beside mediating the cleavage of LexA and UmuD proteins...
  96. ncbi Limited concentration of RecA delays DNA double-strand break repair in Deinococcus radiodurans R1
    Edmond Jolivet
    Institut de Genetique et Microbiologie, CNRS UMR 8621, LRC CEA 42V, Batiment 409, Universite Paris Sud, F 91405 Orsay cedex, France
    Mol Microbiol 59:338-49. 2006
    ..Thus, LexA protein seems to play no major role in the recovery processes after gamma-irradiation in D. radiodurans...
  97. ncbi An exonuclease I-sensitive DNA repair pathway in Deinococcus radiodurans: a major determinant of radiation resistance
    Hari S Misra
    Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
    Mol Microbiol 59:1308-16. 2006
    ....
  98. ncbi Crystal structure of a Rad51 filament
    Adam B Conway
    Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Struct Mol Biol 11:791-6. 2004
    ..Additionally, we show that mutation of His352, which lies at this new interface, markedly disrupts DNA binding...
  99. ncbi Slow induction of RecA by DNA damage in Mycobacterium tuberculosis
    K G Papavinasasundaram
    Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Microbiology 147:3271-9. 2001
    ..A decrease in the ability of LexA to bind to the SOS box was readily detected by 2 h in M. smegmatis, whilst a decrease was not apparent until 18-24 h in M. tuberculosis and then only a very small decrease was observed...
  100. pmc The synapsis event in the homologous pairing of DNAs: RecA recognizes and pairs less than one helical repeat of DNA
    P Hsieh
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 89:6492-6. 1992
    ....
  101. ncbi Recombinational DNA repair in bacteria and the RecA protein
    M M Cox
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    Prog Nucleic Acid Res Mol Biol 63:311-66. 1999
    ..The RecA protein plays a central role in the recombination phase of the process. Among its many activities, RecA protein is a motor protein, coupling the hydrolysis of ATP to the movement of DNA branches...

Research Grants57

  1. UNDERSTANDING HOMOLOGY IN GENETIC RECOMBINATION
    Scott F Singleton; Fiscal Year: 2012
    ..This new knowledge will increase the efficiency of the discovery of small-molecule effectors that address the urgent unmet need to overcome antibiotic resistance in biothreat and other pathogenic bacteria. ..
  2. THE BIOCHEMISTRY OF GENETIC RECOMBINATION/RECA PROTEIN
    Michael M Cox; Fiscal Year: 2013
    ..Ref may eventually be utilized in the genomic engineering of mammalian DNA, in the service of human gene therapy or the generation of mouse gene knockouts. ..
  3. SSB PROTEIN/DNA INTERACTIONS
    Timothy M Lohman; Fiscal Year: 2013
    ..Our basic studies of EcoSSB and its interacting proteins can potentially lead to a new target for the development of new antibiotics. ..
  4. Biochemical Mechanisms and Function of DNA Helicases
    STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2013
    ..Understanding the mechanism and function of these motor proteins is a longstanding goal of this research proposal. ..
  5. NEW GENE THERAPY FOR CONNECTIVE TISSUE DISEASES
    Eric Kmiec; Fiscal Year: 1999
    ..In this proposal, we will extend our preliminary results to more clinically relevant protocols by attempting to correct mutations of genomic sequences involved in several inherited connective tissue diseases. ..
  6. DNA DAMAGE, REPAIR, AND MUTAGENESIS
    IRWIN TESSMAN; Fiscal Year: 1992
    ....
  7. DNA STRUCTURE AND RECOMBINATIONAL INTERMEDIATES
    Brian Johnston; Fiscal Year: 1993
    ..These will then be cut, using either endonuclease VII from phage T4, a resolvase which cuts at D-loops, or a chemical cutting function attached to the oligonucleotide...
  8. MECHANISM OF PROTEIN SPLICING IN MYCOBACTERIUM
    Henry Paulus; Fiscal Year: 2000
    ..Agents that interfere with the splicing of the M. tuberculosis RecA protein may thus constitute a new class of antimycobacterial drugs. ..
  9. RECOMBINATION IN VITRO: ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 1993
    ....
  10. RECOMBINATION IN VITRO--ENZYMOLOGY AND INTERMEDIATES
    CHARLES RADDING; Fiscal Year: 2003
    ..The long term goals are to understand the pathways of recombination mediated by RecA protein and its homologs, and to use that knowledge to facilitate gene targeting and gene therapy. ..
  11. RECA PROTEIN STRUCTURE AND FUNCTION
    KEVIN MCENTEE; Fiscal Year: 1993
    ..These latter experiments will provide additional insight into the mechanisms of recombination in eurkaryotic cells...
  12. TARGETING NATIVE DOUBLE HELIX STRUCTURES AND FUNCTIONS
    DAVID ZARLING; Fiscal Year: 1999
    ....
  13. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2004
    ..coli, and provide potential targets for novel antimicrobials. ..
  14. Site-Specific Recognition Restriction Enzymes
    WILLIAM MACCONNELL; Fiscal Year: 2006
    ..The two components will be tested to determine if they specifically cleave large DNAs. The resulting products, as kits and services, are applicable to 45,000 molecular biology labs worldwide. [unreadable] [unreadable] [unreadable]..
  15. MECHANISTIC STUDIES OF GENETIC RECOMBINATION
    STEPHEN KOWALCZYKOWSKI; Fiscal Year: 1999
    ..Finally, the role of these proteins in resolution of crossover structures will be examined. Knowledge of the mechanism of aberrant chromosomal translocations and may also provide new experimental approaches for gene targeting therapies. ..
  16. AFFINITY PURIFICATION OF LARGE FRAGMENTS OF HUMAN DNA
    David Ward; Fiscal Year: 1992
    ....
  17. RADIOBIOLOGY OF D RADIODURANS--GENETIC APPROACH
    Michael Daly; Fiscal Year: 2001
    ..These studies will delineate the genetics and biochemistry of radioresistance in D. radiodurans. Insight into its strategies may have broad implications for understanding radioresistance and DSB-metabolism throughout nature. ..
  18. RADIATION INDUCED CELLULAR MUTAGENESIS AND DNA REPAIR
    RICHARD BOCKRATH; Fiscal Year: 2001
    ..A newly defined gene and protein activity that influences the consequence(s) of photolyase-CPD complexes in DNA in E. coli will be characterized genetically and physiologically. ..
  19. DNA STRUCTURE AND GENE REGULATION
    Robert Wells; Fiscal Year: 1992
    ..An understanding of the details of genetic expression is fundamental to our ultimate comprehension of normal cellular growth and division as well as disease syndromes characterized by unregulated growth such as cancer...
  20. MECHANISM OF THE RECBCD ENZYME FROM ESCHERICHIA COLI
    DOUGLAS JULIN; Fiscal Year: 1993
    ..The recA protein should be able to promote base-pairing between single-stranded DNA produced by recBCD nuclease or unwinding activity and homologous sequences in the circular molecule...
  21. MECHANISM OF INDUCTION OF VIRAL AND CELLULAR FUNCTION
    David Mount; Fiscal Year: 1993
    ..Finally, the relationship between induction of the SOS regulon and enhanced excision of pyrimidine dimers from the transcribed DNA strand will be investigated...
  22. Structure/function of RecA protein from P. aeruginosa
    Michael Cox; Fiscal Year: 2006
    ..Such proteins may eventually prove useful in efforts to use RecA in gene therapy protocols and to generate crystals of RecA-DNA complexes for structural analysis. ..
  23. GENETIC ANALYSIS OF THE RECA PROTEIN OF E. COLI
    George Weinstock; Fiscal Year: 1991
    ..DNA rearrangements that result from recombination between repeated sequences will also be analyzed after DNA damaging treatments to determine if there may be constraints on recombination to maintain proper chromosome structure...
  24. Biochemical and structural analysis of LexA cleavage
    John Little; Fiscal Year: 2006
    ..RecA-mediated cleavage will be analyzed in more detail. Efforts will be made to solve the structure of a RecA:LexA complex. ..
  25. Polymerization and Activation of Bacterial RecA Proteins
    FREDERICK WHIPPLE; Fiscal Year: 2004
    ..These polymers will be characterized by alternating interface specificities. ..
  26. Functions of the PcrA Helicase in Bacillus anthracis
    Saleem Khan; Fiscal Year: 2007
    ..anthracis. Future development of drugs that specifically inhibit the functions of the PcrA helicase could add to the treatment regimen against B. anthracis and related organisms. [unreadable] [unreadable]..
  27. Targeting E. coli DNA polymerase V to a DNA Lesion
    ERICA SEITZ; Fiscal Year: 2003
    ....
  28. DIFFRACTION ANALYSES OF SS DNA COMPLEXES WITH PROTEINS
    Alexander McPherson; Fiscal Year: 1992
    ..coli topoisomerase 1. Crystals of these proteins will permit us to extend our analyses to other systems and expand our understanding of the principles by which proteins interact with ssDNA...
  29. DNA RECOMBINASE REACTION MECHANISMS
    FLOYD BRYANT; Fiscal Year: 2002
    ..3. To determine the structural origin and mechanistic function of the rate-determining isomerization of the recA-ssDNA complex. 4. To elucidate the mechanistic nature of the coupling between ATP hydrolysis and DNA branch migration. ..
  30. GENETIC RECOMBINATION IN PHAGE AND FUNGI
    FRANKLIN STAHL; Fiscal Year: 2001
    ..We will test other features of the model of Szostak et al. as well as other features of recombination in yeast. ..
  31. DNA REPAIR FUNCTION OF REC A AND PROTEIN DESIGN
    JUKKA KONOLA; Fiscal Year: 1999
    ..This system has allowed the identification of mutants with distinct split phenotypes which will facilitate in the dissection of this repair pathway. ..
  32. RecA Protein as Therapeutic Target Using Peptides & PNAs
    DANIEL CLINE; Fiscal Year: 2008
    ..Several antisense PNAs have been designed and will be tested for their in vitro inhibition of translation. Both peptide and PNA systems will be tested for in vivo activity in E. coli using either biotin or peptide translocation tags. ..
  33. Purchase of Transmission Electron Microscope (TEM)
    Michael Cox; Fiscal Year: 2003
    ..In addition, its operation will be integrated with that of the two other electron microscopes on campus (of more recent vintage), which are located in the veterinary and medical schools. ..
  34. Double strand DNA break repair in D. radiodurans
    Michael Cox; Fiscal Year: 2008
    ..The work has the potential for the identification of entirely novel proteins and pathways for DNA repair. ..
  35. Bacterial Proteins Involved in DNA Repair
    Michael Cox; Fiscal Year: 2007
    ..These experiments will provide new information about critical parts of the pathways leading to replication fork reactivation. The ultimate goal is a complete reconstitution of the pathways with pure enzymes. ..
  36. REGULATORY MECHANISMS IN SMALL DNA PHAGES
    ETHEL TESSMAN; Fiscal Year: 1980
    ..Phage gene products that interact with C protein will be identified. Mutagenesis and UV-reactivation of phage ST-1 will be studied in E. coli K12 tif-1 which produces a heat-activable form of the host recA protein...
  37. DNA AND PROTEIN FLEXIBILITY IN DNA SEQUENCE RECOGNITION
    GERALD KOUDELKA; Fiscal Year: 2001
    ..These studies will also allow to determine the oligomeric form of repressor that searches th DNA for a specific site. ..
  38. HTS for Protein Splicing Inhibitors as TB Drugs
    Henry Paulus; Fiscal Year: 2003
    ....
  39. F.nucleatum in Amniotic Fluid Infection
    Yiping Han; Fiscal Year: 2004
    ..nucleatum for diagnostic purposes, and intervention studies aimed at reducing the incidence of preterm birth. ..
  40. BIOCHEMICAL BASIS OF SOS--INDUCED MUTAGENESIS
    MYRON GOODMAN; Fiscal Year: 2002
    ..Another important goal is to examine the role of pol III in a UmuD'2C-independent pathway for repair of DNA damaged by exposure to ultraviolet radiation. ..
  41. Structural Studies of RecA-DNA Complexes
    Charles Bell; Fiscal Year: 2009
    ..Thus, understanding the mechanism of action of RecA will contribute greatly to our general knowledge of enzymes involved in virtually all aspects of DNA metabolism. ..
  42. DNA Mismatch and Double-Strand Break Repair
    Martin G Marinus; Fiscal Year: 2010
    ..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..
  43. Mechanisms of mistranslation-mediated mutator response
    M Zafri Humayun; Fiscal Year: 2009
    ..2) Test the hypothesis that in Rec- mutA cells there is selective killing of cells undergoing episodic aberrant replication cycles. (3) Test the hypothesis that the TSM phenotype results from episodic hyper-mutagenesis cycles. ..
  44. DNA REPAIR IN DRUG RESISTANCE MUTATION
    Susan Rosenberg; Fiscal Year: 2003
    ....
  45. Localization of Recombination sites in E. coli
    Steven J Sandler; Fiscal Year: 2010
    ..These new reagents will also provide important molecular tools for others in the research community. PHS 398 (Rev. 09/04) Page 2 Principal Investigator/Program Director (Last, first, middle): SANDLER, STEVEN, J ..
  46. Sonoporation as a new genetic tool for F. nucleatum
    Yiping Han; Fiscal Year: 2008
    ..nucleatum will be investigated. Our long term goal is to develop sonoporation into a robust tool for genetic manipulation of microorganisms [unreadable] [unreadable] [unreadable]..
  47. MECHANISMS OF INDUCIBLE ERROR PRONE DNA REPLICATION
    M Humayun; Fiscal Year: 2004
    ..b) Analyze error-prone replication in UVM-induced dnaE(Ts) mutants (defective for pol-Ill) under permissive and restrictive conditions. (c) Purify and characterize the error-prone polymerase activity in UVM-induced cells. ..
  48. FUNCTIONAL GENOMICS STUDY OF TREPONEMA PALLIDUM
    Timothy Palzkill; Fiscal Year: 2006
    ..pallidum antigenic proteins, and iii) establish a large-scale protein-protein interaction network among periplasmic and surface localized proteins. ..
  49. Molecular mechanism of DNA-polymerase choice in vivo
    Susan Rosenberg; Fiscal Year: 2007
    ..coli DNA polymerases, and functions in DNA polymerase choice in vivo: a critical regulatory decision for cell survival of environmental damage, and genomic health. [unreadable] [unreadable] [unreadable]..
  50. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
    ..We will extend genetic analysis of radC to determine what role it may play in repair and recombination. ..
  51. Gordon Research Conference on Mutagenesis, 2002
    MYRON GOODMAN; Fiscal Year: 2002
    ..abstract_text> ..
  52. Reagents for Preparing Structure-Free DNA and RNA
    Howard Gamper; Fiscal Year: 2007
    ....
  53. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010
    ..LAY DESCRIPTION: We will study a protein present in all bacteria that appears to control their growth. This will provide important missing information about how bacteria divide and may provide a target for antibiotics. ..