- Human SLX4 is a Holliday junction resolvase subunit that binds multiple DNA repair/recombination endonucleases
Genome Instability and Carcinogenesis UPR3081 CNRS, Conventionné par l Université d Aix Marseille 2, IGC, IMM 31 chemin Joseph Aiguier, 13402 Marseille, France
Cell 138:78-89. 2009
..We propose that SLX4 acts as a docking platform for multiple structure-specific endonucleases...
- Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair
Jennifer M Svendsen
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
Cell 138:63-77. 2009
..Thus, SLX4 assembles a modular toolkit for repair of specific types of DNA lesions and is critical for cellular responses to replication fork failure...
- SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype
Department of Clinical Genetics, Vrije Universiteit VU Medical Center, Amsterdam, The Netherlands
Nat Genet 43:138-41. 2011
..These individuals, who were previously diagnosed as having Fanconi anemia, add SLX4 as an essential component to the FA-BRCA genome maintenance pathway...
- Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice
Catherine S Branda
Harvard Medical School, Department of Genetics, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Dev Cell 6:7-28. 2004
..By enabling high-fidelity DNA modifications to be induced in vitro or in vivo, these systems have incited a wave of new biology, advancing our understanding of gene function, genetic relationships, development, and disease...
- Chimeric TALE recombinases with programmable DNA sequence specificity
Andrew C Mercer
The Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Nucleic Acids Res 40:11163-72. 2012
Site-specific recombinases are powerful tools for genome engineering...
- Mutations of the SLX4 gene in Fanconi anemia
Laboratory of Genome Maintenance, The Rockefeller University, New York, New York, USA
Nat Genet 43:142-6. 2011
- Mechanisms of site-specific recombination
Nigel D F Grindley
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
Annu Rev Biochem 75:567-605. 2006
..Virtually all identified site-specific recombinases fall into one of just two families, the tyrosine recombinases and the serine recombinases, named after the ..
- Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair
Ivan M Munoz
MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
Mol Cell 35:116-27. 2009
..Depletion of SLX4 causes a decrease in DSB-induced homologous recombination. These data show that SLX4 is a regulator of structure-specific nucleases and that SLX4 and SLX1 are important regulators of genome stability in human cells...
- Aberrant chromosome morphology in human cells defective for Holliday junction resolution
London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
Nature 471:642-6. 2011
- Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia
Gerry P Crossan
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK
Nat Genet 43:147-52. 2011
..The Btbd12 knockout mouse therefore establishes a disease model for Fanconi anemia and genetically links a regulator of nuclease incision complexes to the Fanconi anemia DNA crosslink repair pathway...
- Serine recombinases as tools for genome engineering
William R A Brown
School of Biology, Queens Medical Centre, Nottingham NG7 2UH, UK
Methods 53:372-9. 2011
The serine recombinases differ mechanistically from the tyrosine recombinases and include proteins such as ϕC31 integrase which, unlike Cre and Flp, promote unidirectional reactions...
- Drosophila MUS312 and the vertebrate ortholog BTBD12 interact with DNA structure-specific endonucleases in DNA repair and recombination
Sabrina L Andersen
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Mol Cell 35:128-35. 2009
..Genetic and biochemical evidence described here and in related papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct endonucleases in different recombination and repair contexts...
- Diversity in the serine recombinases
Margaret C M Smith
Institute of Genetics, Queens Medical Centre, University of Nottingham, NG7 2UH, UK
Mol Microbiol 44:299-307. 2002
Most site-specific recombinases fall into one of two families, based on evolutionary and mechanistic relatedness. These are the tyrosine recombinases or lambda integrase family and the serine recombinases or resolvase/invertase family...
- Phage recombinases and their applications
Kenan C Murphy
Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA, USA
Adv Virus Res 83:367-414. 2012
..From the beginning, it was recognized that these recombinases are linked intimately to the mechanisms of phage DNA replication...
- Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice
Center for Regenerative Therapies Dresden, BioInnovationsZentrum Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany
Dis Model Mech 2:508-15. 2009
Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice...
- Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
Chromosoma 116:471-86. 2007
..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
- Zinc-finger recombinase activities in vitro
Marko M Prorocic
Institute of Infection, Immunity and Inflammation, University of Glasgow, GBRC, Glasgow G12 8QQ, Scotland, UK
Nucleic Acids Res 39:9316-28. 2011
Zinc-finger recombinases (ZFRs) are chimaeric proteins comprising a serine recombinase catalytic domain linked to a zinc-finger DNA binding domain...
- G4 resolvase 1 binds both DNA and RNA tetramolecular quadruplex with high affinity and is the major source of tetramolecular quadruplex G4-DNA and G4-RNA resolving activity in HeLa cell lysates
Steven D Creacy
Department of Cancer Biology and the Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
J Biol Chem 283:34626-34. 2008
- Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages
Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 105:1626-31. 2008
..Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the nine other genes when expressed in E. coli carries out oligonucleotide-mediated recombination...
- The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination
Galina V Petukhova
Genetics and Biochemistry Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, 5 Memorial Drive, Bethesda, Maryland 20892, USA
Nat Struct Mol Biol 12:449-53. 2005
..Rad51 and Dmc1 recombinases are the major players in these processes...
- Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications
Hannover Medical School Institute for Experimental Hematology, OE 6960, Carl Neuberg Str 1, D 30625 Hannover, Germany
Gene 515:1-27. 2013
Starting in 1991, the advance of Tyr-recombinases Flp and Cre enabled superior strategies for the predictable insertion of transgenes into compatible target sites of mammalian cells...
- Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis
J Kim Holloway
Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA
PLoS Genet 7:e1002094. 2011
..Taken together, these data indicate that BTBD12 functions throughout gametogenesis to maintain genome stability, possibly by co-ordinating repair processes and/or by linking DNA repair events to the cell cycle via ATM...
- Synapsis in phage Bxb1 integration: selection mechanism for the correct pair of recombination sites
Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
J Mol Biol 349:331-48. 2005
Recombination by site-specific recombinases is a highly concerted process that requires synapsis of the correct pair of DNA substrates...
- Recombinational DNA double-strand breaks in mice precede synapsis
S K Mahadevaiah
Division of Developmental Genetics, National Institute for Medical Research, London, UK
Nat Genet 27:271-6. 2001
..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
- Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation
Department of Molecular, Cellular and Developmental Biology, Howard Hughes Medical Institute, and Department of Genetics, Yale University, New Haven, Connecticut 06520 8103, USA
Genetics 173:1969-81. 2006
..Mer2 does not show significant colocalization with Mre11 or Rec102 and Mer2 does not co-immunoprecipitate with Rec102. We propose that Mer2, Mei4, and Rec114 form a distinct complex required for DSB formation...
- Expanding the scope of site-specific recombinases for genetic and metabolic engineering
The Skaggs Institute for Chemical Biology and the Departments of Chemistry and Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037
Biotechnol Bioeng 111:1-15. 2014
Site-specific recombinases are tremendously valuable tools for basic research and genetic engineering...
- Characterization of a new SCCmec element in Staphylococcus cohnii
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China
PLoS ONE 5:e14016. 2010
..Many SCCmec elements of coagulase-negative staphylococci (CoNS) could not be typed using multiplex PCR. Such a 'non-typable' SCCmec was encountered in a Staphylococcus cohnii isolate...
- Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs
CEA, iBiTecS, F 91191 Gif sur Yvette, France
Nucleic Acids Res 38:3952-62. 2010
..structure-based modelling and gene-context analyses, we provide new views on the global landscape of recombinases in 465 bacteriophages...
- VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
Department of Human Genome Research, Kazusa DNA Research Institute, Graduate School of Pharmaceutical Sciences, Chiba University, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
Nucleic Acids Res 39:e49. 2011
..Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are ..
- Site-specific recombination by phiC31 integrase and other large serine recombinases
Margaret C M Smith
Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK
Biochem Soc Trans 38:388-94. 2010
..Integrases belong either to the lambda Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases...
- The budding yeast mei5 and sae3 proteins act together with dmc1 during meiotic recombination
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06520 8103, USA
Genetics 168:1219-30. 2004
..Taken together, these observations suggest that the Mei5 and Sae3 proteins are accessory factors specific to Dmc1. We speculate that Mei5 and Sae3 are necessary for efficient formation of Dmc1-containing nucleoprotein filaments in vivo...
- Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis
Committee on Genetics, University of Chicago, Cummings Life Science Center, 920 East 58th Street, Chicago, IL 60637, USA
Science 337:1222-5. 2012
..We further provide biochemical evidence that Rad51 acts with Mei5-Sae3 as a Dmc1 accessory factor. Thus, Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis...
- Genome engineering using site-specific recombinases
Andreas F Kolb
Cell Physiology Group, Hannah Research Institute, Ayr, United Kingdom
Cloning Stem Cells 4:65-80. 2002
..Site-specific recombinases have become significant tools in all of these areas...
- G4 resolvase 1 tightly binds and unwinds unimolecular G4-DNA
Department of Cancer Biology and the Comprehensive Cancer Center of Wake Forest University School of Medicine, Winston Salem, USA
Nucleic Acids Res 39:7161-78. 2011
..This report provides evidence that G4R1/RHAU tightly binds and unwinds unimolecular G4-DNA structures...
- Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets
Julia C van Kessel
Pittsburgh Bacteriophage Institute and Department of Biological Sciences, 376 Crawford Hall, 4249 Fifth Ave, University of Pittsburgh, Pittsburgh, PA 15260, USA
Mol Microbiol 67:1094-107. 2008
..This ssDNA recombineering provides a simple assay for comparing the activities of related phage recombinases, and we find that both Escherichia coli RecET and phage lambda Red recombination proteins function ..
- Structure, function, and evolution of linear replicons in Borrelia
Department of Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB T2N 4N1, Canada
Annu Rev Microbiol 64:185-202. 2010
..Telomere resolvases are related to the tyrosine recombinases and ResT can generate the crucial reaction intermediate of this class of enzyme, the Holliday junction...
- Regulation of type 1 fimbriae by unlinked FimB- and FimE-like recombinases in uropathogenic Escherichia coli strain CFT073
Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
Infect Immun 74:1072-83. 2006
..Alignment of the predicted amino acid sequences showed that the five recombinases range in sequence similarity from 63 to 70%...
- Multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in Staphylococcus aureus and coagulase-negative staphylococci from Norway
Anne Merethe Hanssen
Department of Microbiology and Virology, Faculty of Medicine, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
Antimicrob Agents Chemother 51:1671-7. 2007
..The enormous diversity observed in the SCCmec elements implies a large SCCmec reservoir in CoNS...
- The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro
Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1 7 29 Suehiro cho, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
Nat Struct Mol Biol 13:823-30. 2006
Nucleoprotein filaments made up of Rad51 or Dmc1 recombinases, the core structures of recombination, engage in ATP-dependent DNA-strand exchange...
- Characterization of XerC- and XerD-dependent CTX phage integration in Vibrio cholerae
Sarah M McLeod
Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, MA 02111, USA
Mol Microbiol 54:935-47. 2004
..The CTXphi genome lacks an integrase; instead, its integration depends on the chromosome-encoded tyrosine recombinases XerC and XerD. During integration, recombination occurs between regions of homology in CTXphi and the V...
- Molecular basis for enhancement of the meiotic DMC1 recombinase by RAD51 associated protein 1 (RAD51AP1)
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 108:3560-5. 2011
..These results suggest that RAD51AP1 also serves an important role in meiotic homologous recombination...
- The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning
S A Sciochetti
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Bacteriol 181:6053-62. 1999
..Bacillus subtilis ripX gene encodes a protein that has 37 and 44% identity with the XerC and XerD site-specific recombinases of Escherichia coli...
- Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps
Helmholtz Zentrum Munchen, Technische Universitat Munchen, Institut für Entwicklungsgenetik, Ingolstadter Landstrasse 1, 85764 München, Neuherberg, Germany
Nucleic Acids Res 38:e106. 2010
..The use of the RMCE system presented in this work requires one single-cloning step followed by one overnight gateway clonase reaction and subsequent cassette exchange in ES cells with efficiencies of 40% in average...
- Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase
Charles A Gersbach
The Skaggs Institute for Chemical Biology, Department of Molecular Biology, CA, USA
Nucleic Acids Res 39:7868-78. 2011
..This work demonstrates the potential for broad applicability of the ZFR technology in genome engineering, synthetic biology and gene therapy...
- PhiC31 integrase facilitates genetic approaches combining multiple recombinases
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
Methods 53:380-5. 2011
Homologous and site-specific DNA recombination has revolutionized genetic engineering. The reliability of recombinases such as Cre and FLP has allowed scientists to design complex strategies to study gene function in mammals...
- A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells
The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Nucleic Acids Res 41:3937-46. 2013
Zinc-finger recombinases (ZFRs) represent a potentially powerful class of tools for targeted genetic engineering...
- A new group of tyrosine recombinase-encoding retrotransposons
Timothy J D Goodwin
Department of Biochemistry, University of Otago, Dunedin, New Zealand
Mol Biol Evol 21:746-59. 2004
..In addition they contain elements with some surprising features, such as spliceosomal introns and long overlapping open reading frames...
- Roles of two large serine recombinases in mobilizing the methicillin-resistance cassette SCCmec
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, USA
Mol Microbiol 88:1218-29. 2013
..Most variants of SCCmec encode two recombinases, CcrA and CcrB, that belong to the large serine family...
- Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases
Genome Stability Laboratory, Laval University Cancer Research Center, Hotel Dieu de Quebec, 9 McMahon, Quebec City, QC, Canada G1R 2J6
Nucleic Acids Res 35:2719-33. 2007
..that while spHop2-Mnd1 affects spDmc1 specifically, mHop2 or mHop2-Mnd1 stimulates both the hRad51 and hDmc1 recombinases in strand exchange assays. Thus, our results suggest a crucial role for S...
- Catalytic residues of the telomere resolvase ResT: a pattern similar to, but distinct from, tyrosine recombinases and type IB topoisomerases
Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 1N4, Canada
J Biol Chem 279:53699-706. 2004
..have shown that ResT catalyzes hairpin formation through a two-step transesterification similar to tyrosine recombinases and type IB topoisomerases. In the present study we have probed the reaction mechanism of ResT...
- In vitro evolution and analysis of HIV-1 LTR-specific recombinases
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Methods 53:102-9. 2011
..We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)γc(-/-) mice...
- Mutation analysis of the SLX4/FANCP gene in hereditary breast cancer
Clinics of Obstetrics and Gynaecology, Hannover Medical School, Carl Neuberg Str 1, 30625 Hannover, Germany
Breast Cancer Res Treat 130:1021-8. 2011
..G700R substitution could make a minor contribution...
- Synthesis of programmable integrases
Russell M Gordley
Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, BCC 550, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:5053-8. 2009
..We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes with >98% accuracy into ..
- Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51
Mrinal Kanti Bhattacharyya
Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
Int J Parasitol 33:1385-92. 2003
..PfRad51 is the first apicomplexan gene identified that codes for a recombination protein, and it offers an excellent model for studying DNA damage inducible gene expression in such parasites...
- The DEXH protein product of the DHX36 gene is the major source of tetramolecular quadruplex G4-DNA resolving activity in HeLa cell lysates
James P Vaughn
Department of Cancer Biology and the Comprehensive Cancer Center of Wake Forest Medical Center, Winston Salem, North Carolina 27157, USA
J Biol Chem 280:38117-20. 2005
..77% of the enzyme from lysates and reduced G4-DNA resolving activity to 46.0 +/- 0.4% of control, demonstrating that DHX36 protein is responsible for the majority of tetramolecular G4-DNA resolvase activity...
- Method for Bxb1-mediated site-specific integration in planta
Yuan Yeu Yau
Plant Gene Expression Center, USDA ARS and Plant and Microbial Biology, University of California Berkeley, Albany, CA, USA
Methods Mol Biol 701:147-66. 2011
..This method would permit the comparison of different molecular constructs at the same genomic locations...
- Regulation of DNA strand exchange in homologous recombination
J Thomas Holthausen
Department of Cell Biology and Genetics, Cancer Genomics Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
DNA Repair (Amst) 9:1264-72. 2010
..The function of recombinases is controlled by accessory proteins that allow coordination of strand exchange with other steps of homologous ..
- SeLOX--a locus of recombination site search tool for the detection and directed evolution of site-specific recombination systems
Max Planck Institute for the Molecular Cell Biology and Genetics, Dresden, Germany
Nucleic Acids Res 38:W293-8. 2010
Site-specific recombinases have become a resourceful tool for genome engineering, allowing sophisticated in vivo DNA modifications and rearrangements, including the precise removal of integrated retroviruses from host genomes...
- Zinc finger recombinases with adaptable DNA sequence specificity
College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom
PLoS ONE 6:e19537. 2011
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences...
- Directed evolution of recombinase specificity by split gene reassembly
Charles A Gersbach
The Skaggs Institute for Chemical Biology, Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Nucleic Acids Res 38:4198-206. 2010
..To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities...
- Resolution of holliday junctions by RuvABC prevents dimer formation in rep mutants and UV-irradiated cells
Génétique Microbienne, INRA, 78352 Jouy en Josas Cedex, France
Mol Microbiol 37:180-91. 2000
..We conclude that the inviability arising from a high frequency of dimer formation in rep or UV-irradiated cells is only observed in the absence of known enzymes that resolve Holliday junctions...
- FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae
Marie Eve Val
CNRS, Centre de Genetique Moleculaire, UPR 2167, Gif sur Yvette, France
PLoS Genet 4:e1000201. 2008
..coli, chromosome dimers are resolved by the addition of a crossover at a specific site, dif, by two tyrosine recombinases, XerC and XerD. The process is coordinated with cell division through the activity of a DNA translocase, FtsK...
- Measuring chromosome dynamics on different time scales using resolvases with varying half-lives
Richard A Stein
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Mol Microbiol 56:1049-61. 2005
..Rather than the 150 domains detected with the Tn3 resolvase, wild-type cells measured over a 10 min time span have more than 400 domains per genome equivalent, and some gyrase mutants exceed 1000...
- Evolution of programmable zinc finger-recombinases with activity in human cells
Russell M Gordley
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Mol Biol 367:802-13. 2007
Site-specific recombinases are important tools for genomic engineering in many living systems. Applications of recombinases are, however, constrained by the DNA targeting endemic of the recombinase used...
- Distribution of the ACME-arcA gene among meticillin-resistant Staphylococcus haemolyticus and identification of a novel ccr allotype in ACME-arcA-positive isolates
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, PR China
J Med Microbiol 58:731-6. 2009
..The results from this study revealed that MRSH is likely to be one of the potential reservoirs of ACME for Staphylococcus aureus...
- The structure of the excisionase (Xis) protein from conjugative transposon Tn916 provides insights into the regulation of heterobivalent tyrosine recombinases
Department of Chemistry and Biochemistry, UCLA DOE Institute of Genomics and Proteomics, and the Molecular Biology Institute, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095 1570, USA
J Mol Biol 347:11-25. 2005
Heterobivalent tyrosine recombinases play a prominent role in numerous bacteriophage and transposon recombination systems...
- Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC
Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
Antimicrob Agents Chemother 48:2637-51. 2004
..of the mec gene complex, which encodes methicillin resistance, and the ccr gene complex, which encodes the recombinases responsible for its mobility...
- Structure-guided reprogramming of serine recombinase DNA sequence specificity
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 108:498-503. 2011
..These results demonstrate that unique resolvase and invertase derivatives can be developed to site-specifically modify the human genome in the context of zinc-finger recombinase fusion proteins...
- Transgene manipulation in zebrafish by using recombinases
Department of Life Sciences, Indiana State University, Terre Haute, Indiana 47809, USA
Methods Cell Biol 77:363-79. 2004
..g., phiC31 integrase) can improve the utility of this system. After the fish system is fully established, it would be interesting to explore its application to genome engineering in other organisms...
- Strand selection by the tyrosine recombinases
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Prog Nucleic Acid Res Mol Biol 80:1-42. 2005
- Norfloxacin-induced DNA cleavage occurs at the dif resolvase locus in Escherichia coli and is the result of interaction with topoisomerase IV
Department of Pathology, University of Utah, Salt Lake City 84112, USA
Mol Microbiol 33:1027-36. 1999
..This interaction required XerC and XerD, but did not require the C-terminal region of FtsK or cell division...
- Efficient DNA cassette exchange in mouse embryonic stem cells by staggered positive-negative selection
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0615, USA
Genesis 39:256-62. 2004
..This selection strategy, by facilitating more efficient production of ES cell clones with various replacement DNA cassettes, should accelerate targeted repetitive introduction of gene modifications into the mouse...
- Xer1-mediated site-specific DNA inversions and excisions in Mycoplasma agalactiae
Institute of Bacteriology, Mycology and Hygiene IBMH, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, Austria
J Bacteriol 192:4462-73. 2010
..Unless there are specific regulatory mechanisms preventing such excisions, this might be the cost that the pathogen has to render at the population level for maintaining this high-frequency phase variation machinery...
- Hallmarks of homology recognition by RecA-like recombinases are exhibited by the unrelated Escherichia coli RecT protein
Laboratoire de Genetique Microbienne, INRA, 78352 Jouy en Josas Cedex, France
EMBO J 22:324-34. 2003
..Taken together, our results show that RecT shares fundamental homology-recognition properties with the RecA homologs, and provide new insights on an underlying universal mechanism of homologous recognition...
- Arginine as a general acid catalyst in serine recombinase-mediated DNA cleavage
Ross A Keenholtz
From the Department of Biochemistry and Molecular Biology and
J Biol Chem 288:29206-14. 2013
Members of the serine family of site-specific DNA recombinases use an unusual constellation of amino acids to catalyze the formation and resolution of a covalent protein-DNA intermediate...
- Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa
Department of Surgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA
BMC Mol Biol 9:20. 2008
- Characterization of a complex context containing mecA but lacking genes encoding cassette chromosome recombinases in Staphylococcus haemolyticus
Center of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, China
BMC Microbiol 13:64. 2013
..However, the mecA gene might not be carried by a SCCmec in a Staphylococcus haemolyticus clinical isolate, WCH1, as no cassette chromosome recombinase genes were detected. Therefore, the genetic context of mecA in WCH1 was investigated...
- HUH site-specific recombinases for targeted modification of the human genome
Departamento de Biología Molecular Universidad de Cantabria and IBBTEC UC, CSIC, SODERCAN, Santander, Spain
Trends Biotechnol 31:305-12. 2013
Site-specific recombinases (SSRs) have been crucial in the development of mammalian transgenesis...
- A phylogenomic study of DNA repair genes, proteins, and processes
J A Eisen
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Mutat Res 435:171-213. 1999
..We use this phylogenomic analysis to study the evolution of repair proteins and processes and to predict the repair phenotypes of those species for which we now know the complete genome sequence...
- Lactococcal phage genes involved in sensitivity to AbiK and their relation to single-strand annealing proteins
Julie D Bouchard
Departement de Biochimie et de Microbiologie, Faculté de Sciences et de Génie, Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada G1K 7P4
J Bacteriol 186:3649-52. 2004
..Four nonsimilar proteins involved in the sensitivity to AbiK (Sak) were identified. Two of these Sak proteins are related to Erf and RAD52, single-strand annealing proteins involved in homologous recombination...
- The mu transpososome through a topological lens
Rasika M Harshey
Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, TX, USA
Crit Rev Biochem Mol Biol 41:387-405. 2006
..This methodology has also revealed the order and dynamics of association of the three interacting DNA sites, as well as the role of the enhancer in assembly of the Mu transpososome...
- C-terminal interactions between the XerC and XerD site-specific recombinases
A J Spiers
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Mol Microbiol 32:1031-42. 1999
..By extrapolating the Cre DNA recombinase structure to the related site-specific recombinases XerC and XerD, it is predicted that the extreme C-termini of XerC and XerD interact with alpha-helix M in XerD ..
- Strategies for the use of site-specific recombinases in genome engineering
Julie R Jones
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA
Methods Mol Med 103:245-57. 2005
..Strategies that make use of site-specific recombinases such as Cre and/or Flp have been developed in recent years to overcome these limitations...
- Action of site-specific recombinases XerC and XerD on tethered Holliday junctions
L K Arciszewska
Department of Biochemistry, University of Oxford, UK
EMBO J 16:3731-43. 1997
In Xer site-specific recombination, two related recombinases, XerC and XerD, mediate the formation of recombinant products using Holliday junction-containing DNA molecules as reaction intermediates...
- Two site-specific recombinases are implicated in phenotypic variation and competitive rhizosphere colonization in Pseudomonas fluorescens
Departamento de Biologia, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
Microbiology 151:975-83. 2005
..variants under both culture and rhizosphere colonization conditions, implying that both site-specific recombinases are involved in phenotypic variation...
- Site-specific recombinase genes in three Shigella subgroups and nucleotide sequences of a pinB gene and an invertible B segment from Shigella boydii
Department of Biology, Faculty of Science, Okayama University, Japan
J Bacteriol 173:4079-87. 1991
..The defective pinF gene is suggested to hae the same origin as P-pin on e14 by the restriction map of the fragment cloned from a Pin+ transductant that was obtained in transduction from S. flexneri to E. coli delta pin...
- Resolving integral questions in site-specific recombination
Phoebe A Rice
Nat Struct Mol Biol 12:641-3. 2005
- New insights into host factor requirements for prokaryotic beta-recombinase-mediated reactions in mammalian cells
Departamento de Inmunologia y Oncologia and Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, , Cantoblanco, E-28049 Madrid, Spain
J Biol Chem 276:16257-64. 2001
..These data demonstrate the existence of cellular factors different from HMG-1 that can act as helpers for beta-recombinase activity in the eukaryotic environment...
- Chimeric recombinases with designed DNA sequence recognition
Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland
Proc Natl Acad Sci U S A 100:8688-91. 2003
..We describe chimeric recombinases with a catalytic domain from an activated multiple mutant of the bacterial enzyme Tn3 resolvase, fused to a DNA ..
- Elimination of selection markers from transgenic plants
Friedrich Miescher Institute, Maulbeerstrasse 66, 4058 Basel, Switzerland
Curr Opin Biotechnol 12:139-43. 2001
..Regimes to achieve their efficient elimination, mostly through site-specific recombination or transposition, are being developed...
- Excision of selectable marker genes from transgenic plants
Peter D Hare
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Nat Biotechnol 20:575-80. 2002
..The implementation of these strategies in crops and their further improvement will help to expedite widespread public acceptance of agricultural biotechnology..
- ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome
James G Thomson
Plant Gene Expression Center, 800 Buchanan Street, Albany, CA 94710, USA
Transgenic Res 18:237-48. 2009
- Cryptons: a group of tyrosine-recombinase-encoding DNA transposons from pathogenic fungi
Timothy J D Goodwin
Department of Biochemistry, University of Otago, Cumberland Street, Dunedin, New Zealand
Microbiology 149:3099-109. 2003
..Sequence comparisons suggest that cryptons may be related to the DIRS1 and Ngaro1 groups of tyrosine-recombinase-encoding retrotransposons...
- FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation
F X Barre
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, OX1 3QU, UK
Genes Dev 14:2976-88. 2000
..region of the chromosome, converts dimers to monomers through the sequential action of the XerC and XerD recombinases. Chromosome dimer resolution requires that dif is positioned correctly in the chromosome, and the activity of ..
- Challenging a paradigm: the role of DNA homology in tyrosine recombinase reactions
Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington 98125, USA
Microbiol Mol Biol Rev 73:300-9. 2009
..catalyzing site-specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp recombinases, is the ability to recombine substrates sharing very limited DNA sequence identity...
- DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination
Department of Microbiology, University of Illinois, Urbana Champaign, B103, Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801 3709, USA
Proc Natl Acad Sci U S A 98:8461-8. 2001
..In vitro properties of the eukaryotic recombinases suggest a bigger role for single-strand annealing in the eukaryotic recombinational repair.
- FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, United Kingdom
Cell 108:195-205. 2002
..a complete Xer recombination reaction between dif sites by switching the state of activity of the XerCD recombinases so that XerD makes the first pair of strand exchanges to form Holliday junctions that are then resolved by XerC...
- Crystal structure of the site-specific recombinase, XerD
H S Subramanya
Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
EMBO J 16:5178-87. 1997
- Flexibility in DNA recombination: structure of the lambda integrase catalytic core
H J Kwon
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston MA 02115, USA
Science 276:126-31. 1997
Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input...
- Tyrosine site-specific recombinases mediate DNA inversions affecting the expression of outer surface proteins of Bacteroides fragilis
Katja G Weinacht
Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Mol Microbiol 53:1319-30. 2004
- Interactions of the Caulobacter crescentus XerC and XerD recombinases with the E. coli dif site
Departement de Microbiologie et Immunologie, Universite de Montreal, C P 6128 Succ Centre Ville, Montreal, QC, Canada H3C3J7
FEMS Microbiol Lett 222:257-62. 2003
..These dimers are then resolved by the action of the XerC and XerD recombinases, which act on the chromosomal dif site in the presence of the FtsK cell division protein...