mixed function oxygenases

Summary

Summary: Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.

Top Publications

  1. pmc The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
  2. ncbi Gibberellin metabolism: new insights revealed by the genes
    P Hedden
    Crop Production and Improvement Dept, IACR Long Ashton Research Station, University of Bristol Dept of Agricultural Sciences, Long Ashton, Bristol, UK BS41 9AF
    Trends Plant Sci 5:523-30. 2000
  3. ncbi Identification of the gene for vitamin K epoxide reductase
    Tao Li
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nature 427:541-4. 2004
  4. pmc FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity
    P C Mahon
    Institute of Genetic Medicine, Departments of Pediatrics and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 3914, USA
    Genes Dev 15:2675-86. 2001
  5. ncbi AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
    Pål Ø Falnes
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, National Hospital, 0027 Oslo, Norway
    Nature 419:178-82. 2002
  6. ncbi FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes
    Robert P Hausinger
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    Crit Rev Biochem Mol Biol 39:21-68. 2004
  7. ncbi Randomized trial of genotype-guided versus standard warfarin dosing in patients initiating oral anticoagulation
    Jeffrey L Anderson
    Cardiovascular Department, LDS Hospital, Intermountain Healthcare, Salt Lake City, UT, USA
    Circulation 116:2563-70. 2007
  8. pmc Use of pharmacogenetic and clinical factors to predict the therapeutic dose of warfarin
    B F Gage
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Clin Pharmacol Ther 84:326-31. 2008
  9. pmc Warfarin pharmacogenetics: a single VKORC1 polymorphism is predictive of dose across 3 racial groups
    Nita A Limdi
    University of Alabama at Birmingham, USA
    Blood 115:3827-34. 2010
  10. ncbi Inactivation of the Fto gene protects from obesity
    Julia Fischer
    Institute for Animal Developmental and Molecular Biology, Heinrich Heine University, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Nature 458:894-8. 2009

Research Grants

Detail Information

Publications290 found, 100 shown here

  1. pmc The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
    ..Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass...
  2. ncbi Gibberellin metabolism: new insights revealed by the genes
    P Hedden
    Crop Production and Improvement Dept, IACR Long Ashton Research Station, University of Bristol Dept of Agricultural Sciences, Long Ashton, Bristol, UK BS41 9AF
    Trends Plant Sci 5:523-30. 2000
    ..A future challenge will be to dissect the complex, interacting pathways that mediate the regulation of gibberellin metabolism...
  3. ncbi Identification of the gene for vitamin K epoxide reductase
    Tao Li
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nature 427:541-4. 2004
    ..The expressed enzyme is 163 amino acids long, with at least one transmembrane domain. Identification of the VKOR gene extends our understanding of blood clotting, and should facilitate development of new anticoagulant drugs...
  4. pmc FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity
    P C Mahon
    Institute of Genetic Medicine, Departments of Pediatrics and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 3914, USA
    Genes Dev 15:2675-86. 2001
    ..Involvement of VHL in association with FIH-1 provides a unifying mechanism for the modulation of HIF-1alpha protein stabilization and transcriptional activation in response to changes in cellular O(2) concentration...
  5. ncbi AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
    Pål Ø Falnes
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, National Hospital, 0027 Oslo, Norway
    Nature 419:178-82. 2002
    ..This mechanism represents a new pathway for DNA repair and the third type of DNA damage reversal mechanism so far discovered...
  6. ncbi FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes
    Robert P Hausinger
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    Crit Rev Biochem Mol Biol 39:21-68. 2004
    ....
  7. ncbi Randomized trial of genotype-guided versus standard warfarin dosing in patients initiating oral anticoagulation
    Jeffrey L Anderson
    Cardiovascular Department, LDS Hospital, Intermountain Healthcare, Salt Lake City, UT, USA
    Circulation 116:2563-70. 2007
    ..Pharmacogenetic-guided dosing of warfarin is a promising application of "personalized medicine" but has not been adequately tested in randomized trials...
  8. pmc Use of pharmacogenetic and clinical factors to predict the therapeutic dose of warfarin
    B F Gage
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Clin Pharmacol Ther 84:326-31. 2008
    ..To facilitate use of these pharmacogenetic and clinical algorithms, we developed a nonprofit website, http://www.WarfarinDosing.org...
  9. pmc Warfarin pharmacogenetics: a single VKORC1 polymorphism is predictive of dose across 3 racial groups
    Nita A Limdi
    University of Alabama at Birmingham, USA
    Blood 115:3827-34. 2010
    ..Thus, clinicians should recognize that, although at a population level, the contribution of VKORC1 toward dose requirements is higher in whites than in nonwhites; genotype predicts similar dose requirements across racial groups...
  10. ncbi Inactivation of the Fto gene protects from obesity
    Julia Fischer
    Institute for Animal Developmental and Molecular Biology, Heinrich Heine University, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Nature 458:894-8. 2009
    ..Taken together, these experiments provide, to our knowledge, the first direct demonstration that Fto is functionally involved in energy homeostasis by the control of energy expenditure...
  11. pmc Analysis of the developmental roles of the Arabidopsis gibberellin 20-oxidases demonstrates that GA20ox1, -2, and -3 are the dominant paralogs
    Andrew R G Plackett
    Plant Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom
    Plant Cell 24:941-60. 2012
    ..Some phenotypic recovery of late flowers in GA-deficient mutants, including ga1-3, indicated the involvement of non-GA pathways in floral development...
  12. pmc A genome-wide association study confirms VKORC1, CYP2C9, and CYP4F2 as principal genetic determinants of warfarin dose
    Fumihiko Takeuchi
    Wellcome Trust Sanger Institute, Hinxton, UK
    PLoS Genet 5:e1000433. 2009
    ..5% of dose variance. These GWAS results provide further impetus for conducting large-scale trials assessing patient benefit from genotype-based forecasting of warfarin dose...
  13. pmc A mutation in human CMP-sialic acid hydroxylase occurred after the Homo-Pan divergence
    H H Chou
    Glycobiology Program, Divisions of Hematology Oncology and Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 95:11751-6. 1998
    ..Also, the expression of Neu5Gc previously reported in human fetuses and tumors as well as the traces detected in some normal adult humans must be mediated by an alternate pathway...
  14. pmc Potential sites of bioactive gibberellin production during reproductive growth in Arabidopsis
    Jianhong Hu
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Plant Cell 20:320-36. 2008
    ..The immediate upregulation of GA3ox1 and GA3ox4 after anthesis suggests that pollination and/or fertilization is a prerequisite for de novo GA biosynthesis in fruit, which in turn promotes initial elongation of the silique...
  15. ncbi Cytochrome P450 2C9 (CYP2C9) and vitamin K epoxide reductase (VKORC1) genotypes as determinants of acenocoumarol sensitivity
    Laurent Bodin
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Recherche Scientifique, University Rene Descartes, Paris, France
    Blood 106:135-40. 2005
    ....
  16. ncbi Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development
    Melissa G Mitchum
    Developmental, Cell, and Molecular Biology Group, Department of Botany, Box 91000, Duke University, Durham, NC 27708 1000, USA
    Plant J 45:804-18. 2006
    ....
  17. ncbi A polymorphism in the VKORC1 gene is associated with an interindividual variability in the dose-anticoagulant effect of warfarin
    Giovanna D'Andrea
    Unita di Aterosclerosi e Trombosi, Istituto di Ricovero e Cura a Carattere Scientifico, S Giovanni Rotondo, Foggia, Italy
    Blood 105:645-9. 2005
    ..353) of the interindividual variability. Genetic variants of the VKORC1 gene locus modulate the mean daily dose of drug prescribed to acquire the target anticoagulation intensity...
  18. ncbi Genome-wide association study identifies genetic determinants of warfarin responsiveness for Japanese
    Pei Chieng Cha
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Hum Mol Genet 19:4735-44. 2010
    ..Our study also implied that common SNPs other than those in the CYP2C9, VKORC1 and CYP4F2 genes that show strong effect on the therapeutic warfarin dose might not exist...
  19. pmc Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins
    Matthew E Cockman
    Central Proteomics Facility, University of Oxford, Oxford, United Kingdom
    Mol Cell Proteomics 8:535-46. 2009
    ..These findings indicate that asparaginyl hydroxylation is likely to be widespread among the approximately 300 ARD-containing species in the human proteome...
  20. ncbi The impact of CYP2C9 and VKORC1 genetic polymorphism and patient characteristics upon warfarin dose requirements: proposal for a new dosing regimen
    Elizabeth A Sconce
    School of Clinical and Laboratory Sciences, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Blood 106:2329-33. 2005
    ..Based upon the data, a new warfarin dosing regimen has been developed. The validity of the dosing regimen was confirmed in a second cohort of patients on warfarin therapy...
  21. pmc The wp mutation of Glycine max carries a gene-fragment-rich transposon of the CACTA superfamily
    Gracia Zabala
    Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA
    Plant Cell 17:2619-32. 2005
    ....
  22. pmc Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization
    Roberto Orru
    Department of Genetics and Microbiology, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
    J Biol Chem 286:29284-91. 2011
    ....
  23. ncbi Clinical significance of the cytochrome P450 2C19 genetic polymorphism
    Zeruesenay Desta
    Division of Clinical Pharmacology, Indiana University School of Medicine, Wishard Hospital, Indianapolis 46202, USA
    Clin Pharmacokinet 41:913-58. 2002
    ..Finally, many studies have attempted to identify relationships between CYP2C19 genotype and phenotype and susceptibility to xenobiotic-induced disease, but none of these are compelling...
  24. ncbi A genome-wide association study of acenocoumarol maintenance dosage
    Martina Teichert
    Department of Epidemiology, Erasmus Medical Center, Rotterdam, The Netherlands
    Hum Mol Genet 18:3758-68. 2009
    ..Besides age, gender, body mass index and target INR, one polymorphism within each of the VKORC1, CYP2C9, CYP4F2 and CYP2C18 genes could explain 48.8% of acenocoumarol dosage variation...
  25. ncbi Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts
    W J H van Berkel
    Laboratory of Biochemistry, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands
    J Biotechnol 124:670-89. 2006
    ..This review provides an inventory of known flavoprotein monooxygenases belonging to these different enzyme subclasses. Furthermore, the biocatalytic potential of a selected number of flavoprotein monooxygenases is highlighted...
  26. ncbi Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining
    Marco W Fraaije
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Appl Microbiol Biotechnol 66:393-400. 2005
    ..This study demonstrates that, using effective annotation tools, genomes can efficiently be exploited as a source of novel BVMOs...
  27. pmc Carnitine biosynthesis in mammals
    Frederic M Vaz
    Laboratory for Genetic Metabolic Diseases, Departments of Clinical Chemistry and Paediatrics, Emma Children s Hospital, Academic Medical Centre, University of Amsterdam, P O Box 22700, 1100 DE Amsterdam, The Netherlands
    Biochem J 361:417-29. 2002
    ..This review aims to cover the current knowledge of the enzymological, molecular, metabolic and regulatory aspects of mammalian carnitine biosynthesis, with an emphasis on the human and rat...
  28. pmc Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways
    Xiaofeng Zheng
    Departments of Cell and Molecular Biology and Molecular Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 105:3368-73. 2008
    ..In conclusion, the data reveal posttranslational modification of the activated form of the Notch receptor and an intricate mode of cross-coupling between the Notch and hypoxia signaling pathways...
  29. pmc A non-heme iron-mediated chemical demethylation in DNA and RNA
    Chengqi Yi
    Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA
    Acc Chem Res 42:519-29. 2009
    ..The chemical cross-linking method demonstrated here can be applied to trap other labile protein-DNA interactions and can serve as a general strategy for exploring the structural and functional aspects of base-flipping proteins...
  30. ncbi Integration of oxygen signaling at the consensus HRE
    Roland H Wenger
    Institute of Physiology, Center for Integrative Human Physiology, University of Zurich, CH 8057 Zurich, Switzerland
    Sci STKE 2005:re12. 2005
    ....
  31. pmc Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains
    Ming Yang
    Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, UK
    FEBS J 278:1086-97. 2011
    ..NMR and crystallographic analyses show that the histidinyl hydroxylation occurs at the β-position. The results further expand the scope of FIH-catalysed hydroxylations...
  32. pmc Analysis of substrate access to active sites in bacterial multicomponent monooxygenase hydroxylases: X-ray crystal structure of xenon-pressurized phenol hydroxylase from Pseudomonas sp. OX1
    Michael S McCormick
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    Biochemistry 50:11058-69. 2011
    ....
  33. ncbi A warfarin-dosing model in Asians that uses single-nucleotide polymorphisms in vitamin K epoxide reductase complex and cytochrome P450 2C9
    Lai San Tham
    Department of Haematology Oncology, National University Hospital, and Translational Interface, Oncology Research Institute, Singapore
    Clin Pharmacol Ther 80:346-55. 2006
    ..The aims of this study were to derive a pharmacogenetics-based dosing algorithm by use of retrospective information and to validate it through a data-splitting method in a separate cohort of equal size...
  34. ncbi Association of VKORC1 -1639 G>A polymorphism with carotid intima-media thickness in type 2 diabetes mellitus
    Anna Tavridou
    Lab of Pharmacology, Medical School, Democritus University of Thrace, Dragana Campus, 68100 Alexandroupolis, Greece
    Diabetes Res Clin Pract 94:236-41. 2011
    ..We examined a potential association between VKORC1 -1639 G>A polymorphism and T2DM and, also, the association of this polymorphism with carotid intima-media thickness (cIMT)...
  35. ncbi VKORC1 and CYP2C9 genotype and patient characteristics explain a large proportion of the variability in warfarin dose requirement among children
    Tina T Biss
    Institute of Cellular Medicine, Newcastle upon Tyne, United Kingdom
    Blood 119:868-73. 2012
    ..A pharmacogenomic approach to warfarin dosing has the potential to improve the efficacy and safety of warfarin therapy in children. However, algorithms should be derived from data in children if their potential benefit is to be realized...
  36. ncbi A novel functional VKORC1 promoter polymorphism is associated with inter-individual and inter-ethnic differences in warfarin sensitivity
    Hsiang Yu Yuan
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    Hum Mol Genet 14:1745-51. 2005
    ..The differences in allele frequencies of A/G allele and its levels of VKORC1 promoter activity may underscore the inter-individual differences in warfarin dosage as well as inter-ethnic differences between Chinese and Caucasians...
  37. pmc Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification
    E C Wolff
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4340, USA
    Amino Acids 33:341-50. 2007
    ....
  38. ncbi CYP98A3 from Arabidopsis thaliana is a 3'-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway
    G Schoch
    Department of Plant Stress Response, Institute of Plant Molecular Biology, CNRS UPR 2357, 28 rue Goethe, F 67083 Strasbourg cedex, France
    J Biol Chem 276:36566-74. 2001
    ..This hydroxylation occurs on depsides, the function of which was so far not understood, revealing an additional and unexpected level of networking in lignin biosynthesis...
  39. pmc Structural consequences of effector protein complex formation in a diiron hydroxylase
    Lucas J Bailey
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 105:19194-8. 2008
    ..This description provides insight into the functional role of effector protein binding in all carboxylate-bridged diiron hydroxylases...
  40. pmc Exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB
    Deyu Li
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 134:8896-901. 2012
    ..The observation that AlkB can demethylate m6A in vitro suggests a role for AlkB in regulation of important cellular functions in vivo...
  41. pmc Group-specific monitoring of phenol hydroxylase genes for a functional assessment of phenol-stimulated trichloroethylene bioremediation
    H Futamata
    Marine Biotechnology Institute, Kamaishi Laboratories, Heita, Kamaishi City, Iwate 026-0001, Japan
    Appl Environ Microbiol 67:4671-7. 2001
    ..It is also suggested that phenol biostimulation could become a powerful TCE bioremediation strategy when bacteria possessing group I LmPHs are selectively stimulated...
  42. pmc The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy
    Prajal H Patel
    Program in Developmental Biology, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    J Cell Biol 185:1181-94. 2009
    ..Our data also indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis...
  43. pmc Genome-wide characterization of the lignification toolbox in Arabidopsis
    Jeroen Raes
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B 9052 Gent, Belgium
    Plant Physiol 133:1051-71. 2003
    ..Together, these data describe the full complement of monolignol biosynthesis genes in Arabidopsis, provide a unified nomenclature, and serve as a basis for further functional studies...
  44. pmc An integrative method for scoring candidate genes from association studies: application to warfarin dosing
    Nicholas P Tatonetti
    Biomedical Informatics Training Program, Stanford University School of Medicine, Stanford, CA, USA
    BMC Bioinformatics 11:S9. 2010
    ..We then define a summary score for each gene based on allele frequencies and train linear and logistic regression classifiers to predict drug response phenotypes...
  45. ncbi Distinct octopamine cell population residing in the CNS abdominal ganglion controls ovulation in Drosophila melanogaster
    Maria Monastirioti
    Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, 71110 Heraklion, Crete, Greece
    Dev Biol 264:38-49. 2003
    ..The above results point to a neuronal focus that can synthesize and release octopamine in specific sites of the female reproductive system where the amine is required to trigger ovulation...
  46. pmc Mechanism and regulation of the Two-component FMN-dependent monooxygenase ActVA-ActVB from Streptomyces coelicolor
    Julien Valton
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    J Biol Chem 283:10287-96. 2008
    ..These results suggest that, in Streptomyces coelicolor, the reductase component ActVB can act as a regulatory component of the monooxygenase activity by controlling the transfer of reduced FMN to the monooxygenase...
  47. pmc New genetic variant that might improve warfarin dose prediction in African Americans
    Hedi Schelleman
    Center for Clinical Epidemiology and Biostatistics, Department of Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Pittsburgh, PA 19104 6021, USA
    Br J Clin Pharmacol 70:393-9. 2010
    ..However, these factors seem to explain less of the dose variability in African Americans who have a lower prevalence of the CYP2C9*2 and *3 and VKORC1 1173T alleles...
  48. ncbi ZmGA3ox2, a candidate gene for a major QTL, qPH3.1, for plant height in maize
    Feng Teng
    National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
    Plant J 73:405-16. 2013
    ..The successfully isolated qPH3.1 enriches our knowledge on the genetic basis of plant height in maize, and provides an opportunity for improvement of plant architecture in maize breeding...
  49. ncbi Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis
    Yue Hua Xiao
    Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture of China, Biotechnology Research Center Southern Campus, Southwest University, Tiansheng Road 216, Beibei, Chongqing 400716, PR China
    J Plant Physiol 167:829-37. 2010
    ..Our results suggest that GA 20-oxidase is involved in fiber development by regulating GA levels, and corresponding genes might be employed as target genes for the manipulation of fiber initiation and elongation in cotton...
  50. ncbi Correlating structure with function in bacterial multicomponent monooxygenases and related diiron proteins
    Matthew H Sazinsky
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Acc Chem Res 39:558-66. 2006
    ..Selected findings are compared and contrasted with the properties of other carboxylate-bridged diiron proteins, revealing common structural and functional themes...
  51. ncbi Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis
    Steven D Doig
    Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK
    Biotechnol Prog 18:1039-46. 2002
    ....
  52. ncbi Genetic polymorphisms of cytochromes P450: CYP2C9, CYP2C19, and CYP2D6 in Croatian population
    Nada Bozina
    University Institute of Laboratory Diagnosis, Zagreb University Hospital Center and School of Medicine, Zagreb, Croatia
    Croat Med J 44:425-8. 2003
    ..To determine the prevalence of most common mutations of cytochrome P450 (CYP), ie, allelic variants of CYP2C9, CYP2C19, and CYP2D6, and to predict genotype frequency in the Croatian population...
  53. ncbi Baeyer-Villiger monooxygenases: recent advances and future challenges
    Daniel E Torres Pazmiño
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Curr Opin Chem Biol 14:138-44. 2010
    ..This review provides an overview on the recently gained knowledge on BVMOs and sketches the outlook for future industrial applications of these unique oxidative biocatalysts...
  54. ncbi Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation
    Qunrui Li
    School of Life Sciences, Northeast Normal University, 130024, Changchun, China
    Transgenic Res 19:1053-68. 2010
    ..We discuss the impact of paralogs with different expression profiles and functions on the regulation of carotenoid synthesis in corn...
  55. pmc X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. OX1 phenol hydroxylase
    Matthew H Sazinsky
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 45:15392-404. 2006
    ..Comparisons between the ToMOH and PHH structures provide insights into their substrate regiospecificities...
  56. ncbi Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and biotypes
    Merike Merimaa
    Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia
    Arch Microbiol 186:287-96. 2006
    ..Our data suggest that few central routes for the degradation of phenolic compounds may emerge in bacteria as a result of the combination of genetically diverse catabolic genes...
  57. ncbi Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane
    L Zhang
    Department of Medicine and the Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 272:23389-97. 1997
    ..Taken together, these results suggest that junctin, calsequestrin, triadin, and the ryanodine receptor form a quaternary complex that may be required for normal operation of Ca2+ release...
  58. ncbi Genotype-guided dosing of coumarin derivatives: the European pharmacogenetics of anticoagulant therapy (EU-PACT) trial design
    Rianne M F van Schie
    Utrecht University, Faculty of Science, Utrecht, The Netherlands
    Pharmacogenomics 10:1687-95. 2009
    ..The primary outcome measure is the percentage time in the therapeutic range for international normalized ratio. This report describes the design and protocol for the trial...
  59. pmc Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis
    A L Phillips
    IACR Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, United Kingdom
    Plant Physiol 108:1049-57. 1995
    ....
  60. ncbi Modulation of differentiation-related gene 1 expression by cell cycle blocker mimosine, revealed by proteomic analysis
    Zizheng Dong
    Department of Pharmacology and Toxicology, Indiana University Cancer Center and Walther Oncology Center Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Cell Proteomics 4:993-1001. 2005
    ..Because induction of Drg-1 is an early event after mimosine treatment and is observed before a notable reduction in the steady-state level of mature eIF5A, eIF5A does not appear to be involved in the modulation of Drg-1 expression...
  61. ncbi Recent developments in the application of Baeyer-Villiger monooxygenases as biocatalysts
    Gonzalo de Gonzalo
    Laboratory of Biochemistry, University of Groningen, Groningen, The Netherlands
    Chembiochem 11:2208-31. 2010
    ....
  62. ncbi Ethnic differences in the VKORC1 gene polymorphism and an association with warfarin dosage requirements in cardiovascular surgery patients
    Kenji Nakai
    Iwate Medical University, Department of Laboratory Medicine, Morioka 020 8505, Japan
    Pharmacogenomics 8:713-9. 2007
    ....
  63. pmc Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis
    Hanna M Dudek
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    Appl Environ Microbiol 77:5730-8. 2011
    ..These findings will aid in more dedicated and effective redesign of PAMO and related monooxygenases toward an expanded substrate scope...
  64. ncbi Optimization of an in vivo plant P450 monooxygenase system in Saccharomyces cerevisiae
    Hanxiao Jiang
    School of Chemical Engineering, FRNY Hall, Purdue University, West Lafayette, Indiana 47907, USA
    Biotechnol Bioeng 85:130-7. 2004
    ..A small, but significant (P < 0.05) increase in whole cell F5H activity was observed following ALA addition...
  65. ncbi Metabolic engineering of Escherichia coli for improving L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose
    Ana Joyce Muñoz
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, 62210 Cuernavaca, Morelos, Mexico
    J Ind Microbiol Biotechnol 38:1845-52. 2011
    ..6 mg/g/h, L-DOPA yield from glucose of 51.7 mg/g and a final L-DOPA titer of 320 mg/l. In a batch fermentor culture in rich medium this strain produced 1.51 g/l of L-DOPA in 50 h...
  66. ncbi Pharmacogenetic testing of CYP2C9 and VKORC1 alleles for warfarin
    David A Flockhart
    Indiana University School of Medicine, Indianapolis, Indiana, USA
    Genet Med 10:139-50. 2008
    ....
  67. ncbi Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in Acinetobacter calcoaceticus PHEA-2
    Yuquan Xu
    Department of Biotechnology, Institute for Application of Atomic Energy, Chinese Academy of Agricultural Sciences, P O Box 5109, Beijing, 100094, China
    Curr Microbiol 46:235-40. 2003
    ..The organization of the mph and ben operons is different from that described previously...
  68. ncbi Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli
    Takuya Makino
    School of Fisheries Sciences, Kitasato University, Sanriku cho, Ofunato, 022 0101 Japan
    Plant Cell Physiol 49:1867-78. 2008
    ..PCC 6803, Anabaena sp. PCC 7120 and N. punctiforme PCC 73102, based on the present and previous findings. This explains why these cyanobacteria cannot produce astaxanthin and why only Synechocystis sp. PCC 6803 can produce zeaxanthin...
  69. pmc Cloning of the pleiotropic T locus in soybean and two recessive alleles that differentially affect structure and expression of the encoded flavonoid 3' hydroxylase
    Gracia Zabala
    Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801, USA
    Genetics 163:295-309. 2003
    ....
  70. pmc Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases
    Jan B van Beilen
    Institute of Biotechnology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 72:59-65. 2006
    ..Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases...
  71. pmc Overexpression of Fto leads to increased food intake and results in obesity
    Chris Church
    MRC Harwell, Metabolism and Inflammation, Harwell Science and Innovation Campus, Harwell, UK
    Nat Genet 42:1086-92. 2010
    ..Mice with increased Fto expression on a high-fat diet develop glucose intolerance. This study provides the first direct evidence that increased Fto expression causes obesity in mice...
  72. ncbi Localization of flavonoid enzymes in Arabidopsis roots
    D Saslowsky
    Department of Biology, Virginia Tech, Blacksburg, VA 24061 0406, USA
    Plant J 27:37-48. 2001
    ..Staining of flavonoid endproducts with DPBA was consistent with expression of CHS and CHI in these seedlings...
  73. pmc Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis
    A L Silverstone
    Department of Biology, Box 91000, Duke University, Durham, NC 27708 1000, USA
    Plant Cell 13:1555-66. 2001
    ..The effect of rga on GA4 gene expression and the effect of GA on RGA protein level allow us to identify part of the mechanism by which GA homeostasis is achieved...
  74. ncbi Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
    Per Arne Aas
    Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nature 421:859-63. 2003
    ..The different catalytic properties and the different subnuclear localization patterns shown by the human homologues indicate that hABH2 and hABH3 have distinct roles in the cellular response to alkylation damage...
  75. ncbi Regulation of hypoxia-inducible factor 1 by prolyl and asparaginyl hydroxylases
    Kiichi Hirota
    Department of Anesthesia, Kyoto University Hospital, Kyoto University, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 338:610-6. 2005
    ..In this review, we will summarize recent progress in elucidating the molecular mechanisms of HIF-1 activation, focusing on the role of oxygen-dependent prolyl and asparaginyl hydroxylases in hypoxia signal transduction...
  76. pmc Characterization of the beta-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice
    Hao Du
    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China
    Plant Physiol 154:1304-18. 2010
    ..We conclude that the DSM2 gene significantly contributes to control of the xanthophyll cycle and ABA synthesis, both of which play critical roles in the establishment of drought resistance in rice...
  77. pmc Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis
    M Ruegger
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    Plant Physiol 119:101-10. 1999
    ..In contrast, the positive regulatory function of the downstream region is not required for F5H transcript or sinapoylcholine accumulation in embryos...
  78. ncbi VKORC1 haplotypes and their impact on the inter-individual and inter-ethnical variability of oral anticoagulation
    Christof Geisen
    Institute of Transfusion Medicine and Immunohaematology, Department of Molecular Haemostasis, DRK Blood Donor Service Baden Wuerttemberg, Hessen, Frankfurt am Main, Germany
    Thromb Haemost 94:773-9. 2005
    ....
  79. pmc ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice
    Yongyou Zhu
    National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Plant Cell 18:442-56. 2006
    ..The identification of Eui as a GA catabolism gene provides additional evidence that the GA metabolism pathway is a useful target for increasing the agronomic value of crops...
  80. pmc Use of array CGH to detect exonic copy number variants throughout the genome in autism families detects a novel deletion in TMLHE
    Patricia B S Celestino-Soper
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Mol Genet 20:4360-70. 2011
    ..gov/geo/, date last accessed on 30 August 2011). Genboree accession: http://genboree.org/java-bin/gbrowser.jsp?refSeqId=1868&entryPointId=chr17&from=53496072&to=53694382&isPublic=yes, date last accessed on 30 August 2011...
  81. pmc Identification of the gene encoding alkylglycerol monooxygenase defines a third class of tetrahydrobiopterin-dependent enzymes
    Katrin Watschinger
    Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, A 6020 Innsbruck, Austria
    Proc Natl Acad Sci U S A 107:13672-7. 2010
    ..This sequence assignment suggests that alkylglycerol monooxygenase forms a distinct third group among tetrahydrobiopterin-dependent enzymes...
  82. ncbi Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat
    Nigel E J Appleford
    Rothamsted Research, AL5 2JN Harpenden, Herts, UK
    Planta 223:568-82. 2006
    ..In contrast to TaGA20ox1, TaGA3ox2 is expressed in internodes, as well as nodes and the ear of the elongating stem. It is also highly expressed in developing and germinated embryos...
  83. pmc Molecular cloning of GA 2-oxidase3 from spinach and its ectopic expression in Nicotiana sylvestris
    Dong Ju Lee
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 1312, USA
    Plant Physiol 138:243-54. 2005
    ..It is concluded that genetic manipulation of plant stature by increasing deactivation of precursors of active GA is more advantageous than increased deactivation of bioactive GA1 itself...
  84. pmc Impact of altered gibberellin metabolism on biomass accumulation, lignin biosynthesis, and photosynthesis in transgenic tobacco plants
    Sophia Biemelt
    Institut für Pflanzengenetik and Kulturpflanzenforschung, D 06466 Gatersleben, Germany
    Plant Physiol 135:254-65. 2004
    ..The number of lignified vessels was increased in AtGA20-ox plants pointing to a stimulation of xylem formation while the number of pith cells declined indicating a negative regulation...
  85. ncbi X-ray crystal structure of Escherichia coli taurine/alpha-ketoglutarate dioxygenase complexed to ferrous iron and substrates
    Jonathan M Elkins
    Dyson Perrins Laboratory, Oxford University, South Parks Road, Oxford, England, OX1 3QY, and Departments of Microbiology and Molecular Genetics and Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1011
    Biochemistry 41:5185-92. 2002
    ..These results, along with sequence alignment information, reveal how TauD selects a tetrahedral substrate anion in preference to the planar carboxylate selected by TfdA, providing insight into the mechanism of enzyme catalysis...
  86. pmc Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury
    Peter Hill
    Renal Section, Division of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK
    J Am Soc Nephrol 19:39-46. 2008
    ..Therefore, pharmacological activation of HIF may offer an effective strategy to protect the kidney from ischemic injury...
  87. pmc CYP4F2 is a vitamin K1 oxidase: An explanation for altered warfarin dose in carriers of the V433M variant
    Matthew G McDonald
    Department of Medicinal Chemistry, University of Washington, Seattle, 98195, USA
    Mol Pharmacol 75:1337-46. 2009
    ..Therefore, patients with the rs2108622 polymorphism are likely to have elevated hepatic levels of VK1, necessitating a higher warfarin dose to elicit the same anticoagulant response...
  88. ncbi Effects of CYP2C9 and VKORC1 on INR variations and dose requirements during initial phase of anticoagulant therapy
    Marta Spreafico
    A Bianchi Bonomi Hemophilia and Thrombosis Center, University of Milan and Department of Medicine and Medical Specialties, IRCCS Maggiore Hospital, Mangiagalli and Regina Elena Foundation, Via Pace 9, 20122 Milan, Italy
    Pharmacogenomics 9:1237-50. 2008
    ..However, they have a narrow therapeutic range and show inter- and intra-individual variability in dose requirement, largely conditioned by both environmental and genetic factors...
  89. ncbi Genetic polymorphism of cytochrome P450 2C19 in Mexican Americans: a cross-ethnic comparative study
    Huai Rong Luo
    Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, 66160 7417, USA
    Clin Pharmacol Ther 80:33-40. 2006
    ..Our objectives were to investigate cytochrome P450 (CYP) 2C19 polymorphism in Mexican Americans and compare the findings with those in 4 other ethnic groups...
  90. pmc Metabolite sorting of a germplasm collection reveals the hydroxylase3 locus as a new target for maize provitamin A biofortification
    Ratnakar Vallabhaneni
    Department of Biological Sciences, Lehman College, City University of New York, Bronx, New York 10468, USA
    Plant Physiol 151:1635-45. 2009
    ..A simple PCR assay to track and identify Hydroxylase3 alleles will be valuable for predicting nutritional content in genetically diverse cultivars found worldwide...
  91. ncbi Low serum vitamin K in PXE results in defective carboxylation of mineralization inhibitors similar to the GGCX mutations in the PXE-like syndrome
    Olivier M Vanakker
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Lab Invest 90:895-905. 2010
    ..Although in PXE-like patients this is due to mutations in the GGCX gene, a deficiency of the carboxylation co-factor VK is at the basis of the decreased activity of calcification inhibitors in PXE...
  92. pmc Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b
    N Elango
    Department of Biochemistry, Medical School, University of Minnesota, Minneapolis 55455, USA
    Protein Sci 6:556-68. 1997
    ..Fifteen additional sequence differences distributed randomly over the three chains were also observed, including D alpha 209E, a ligand of one of the irons...
  93. ncbi A new genetic defect in human CYP2C19: mutation of the initiation codon is responsible for poor metabolism of S-mephenytoin
    R J Ferguson
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 284:356-61. 1998
    ..The two cDNAs were also used in an in vitro coupled transcription/translation assay. CYP2C19 protein was translated only from the CYP2C19*1 allele. These data indicate that CYP2C19*4 represents a new PM allele...
  94. ncbi Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study
    John F Carlquist
    Department of Medicine, Division of Cardiology, University of Utah School of Medicine, Cardiovascular Department, LDS Hospital, Salt Lake City, Utah, USA
    J Thromb Thrombolysis 22:191-7. 2006
    ..vitamin K epoxide reductase complex subunit 1-VKORC1). We hypothesized that variants in these two genes explain a substantial proportion of variability in stable warfarin dose and could be used as a basis for improved dosing algorithms...
  95. ncbi Influence of coagulation factor, vitamin K epoxide reductase complex subunit 1, and cytochrome P450 2C9 gene polymorphisms on warfarin dose requirements
    Christina L Aquilante
    Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL 32610, USA
    Clin Pharmacol Ther 79:291-302. 2006
    ....
  96. ncbi Evolution of the soluble diiron monooxygenases
    Joseph G Leahy
    Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    FEMS Microbiol Rev 27:449-79. 2003
    ....
  97. pmc Influence of CYP2C9 and VKORC1 1173C/T genotype on the risk of hemorrhagic complications in African-American and European-American patients on warfarin
    N A Limdi
    Department of Neurology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Clin Pharmacol Ther 83:312-21. 2008
    ..7; 95% CI: 0.7-4.4) or minor (HR 0.8; 95% CI: 0.5-1.3) hemorrhage. The variant CYP2C9 genotype is associated with an increased risk of major hemorrhage, which persists even after stabilization of therapy...
  98. pmc Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay
    Ernst R Werner
    Division of Biological Chemistry, Biocenter, Innsbruck Medical University, A 6020 Innsbruck, Austria
    J Lipid Res 48:1422-7. 2007
    ....
  99. ncbi Alkylation damage in DNA and RNA--repair mechanisms and medical significance
    Finn Drabløs
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    DNA Repair (Amst) 3:1389-407. 2004
    ..An evaluation of the biological effects of environmental mutagens, as well as understanding the mechanism of action and resistance to alkylating drugs require a detailed understanding of DNA repair processes...
  100. ncbi The evolution and function of carotenoid hydroxylases in Arabidopsis
    Joonyul Kim
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Plant Cell Physiol 50:463-79. 2009
    ....
  101. ncbi AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo
    James C Delaney
    Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Struct Mol Biol 12:855-60. 2005
    ..These reactions show a previously unrecognized chemical versatility of AlkB. In mammals, the corresponding AlkB homologs may defend against aging, cancer and oxidative stress...

Research Grants62

  1. REGULATION OF GRANULOCYTE AND MACROPHAGE PRODUCTION
    E Stanley; Fiscal Year: 2001
    ..Further, the mechanistic studies provided interesting clues in reveling their modes of action on the molecular level. Another year of funding is requested for the completion of these studies. ..
  2. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2007
    ..abstract_text> ..
  3. METABOLIC BASIS OF CONJUGATED DIENES TOXICITY
    ADNAN ELFARRA; Fiscal Year: 1992
    ..This will include an investigation of the possible inactivation of enzymes involved in the bioactivation of conjugated dienes by the generated reactive metabolites...
  4. Insecticide resistance in Africa Malaria mosquitoes
    Maureen Coetzee; Fiscal Year: 2004
    ..2. Transfer these technologies to the VCRU and perform limited field trials in order to generate preliminary data that will enable the development of a full R01 application. ..
  5. JUVENILE TROUT IN VITRO MODEL FOR TOXICITY TESTING
    PAULA DEHN; Fiscal Year: 1990
    ..and 2) the suitability of applying this in vitro model to monitoring programs for aquatic systems...
  6. CHEMOPREVENTION OF SKIN TUMORS BY DHEA ANALOGS
    Arthur Schwartz; Fiscal Year: 1993
    ..In addition, we will also determine the effect of 8354 on the NADPH/NADP ratio in epidermis and on the level of 6-phosphogluconate...
  7. CARCINOGEN TESTING: A MOUSE LYMPHOMA MUTATION SYSTEM
    Henry Bourne; Fiscal Year: 1980
    ..The use of multiple markers may make it possible to infer plausible mechanisms of carcinogen action. ..
  8. DISPOSITION METABOLISM & TOXICITY OF HALOALDEHYDES
    DEAN CARTER; Fiscal Year: 1992
    ..If the compounds inhibit aldehyde dehydrogenase like trichloroacetaldehyde, then enzyme isolation and purification experiments will attempt to identify the enzymes responsible for metabolism of the chloroaldehydes...
  9. HISTOCOMPATIBILITY SYSTEMS AND AGING
    Roy Walford; Fiscal Year: 1990
    ....
  10. ROLE OF CYTOCHROMES P450 IN ACETAMINOPHEN NEPHROTOXICITY
    JOAN TARLOFF; Fiscal Year: 1991
    ..These experiments will assist in establishing the pathways involved in APAP bioactivation in the kidney and will clarify the role of cytochrome P450 in the development of APAP nephrotoxicity...
  11. MODIFICATION OF BILIARY EXCRETION BY TOXIC CHEMICALS
    HARIHARA MEHENDALE; Fiscal Year: 1980
    ....
  12. MAJOR HISTOCOMPATIBILTY COMPLEX--AGING & TRANSGENIC MICE
    Rita Effros; Fiscal Year: 1992
    ..Further inbreeding of Peromyscus and serologic and molecular genetic studies of Peromyscus MHC will also be done, as adjunct to the above studies...
  13. Pharmacogenetic Optimization of Anticoagulation Therapy
    Nita Limdi; Fiscal Year: 2007
    ..With the award of the K23 grant, structured training, guidance of mentors, environment and institutional support provided by the University, I will develop the skills necessary for a productive career in pharmacogenetics research. ..
  14. Vitamin K, Bone, and Arterial Calcification
    Reidar Wallin; Fiscal Year: 2006
    ..The proposed work will provide basic knowledge of BMP-2 growth factor mediated calcification as it is linked to the function of a fat-soluble vitamin (vitamin K) and biosynthesis of vitamin K-dependent proteins. ..
  15. Beta-receptors and cardiovascular pharmacogenomics
    Julie Johnson; Fiscal Year: 2006
    ..abstract_text> ..
  16. Beta-adrenoceptor genetic polymorphisms and obesity
    Julie Johnson; Fiscal Year: 2002
    ..Additionally, genotyping individuals early in age may help to identify those at increased risk of obesity prior to them becoming obese so that they may take appropriate preventive measures. ..
  17. VITAMIN K, METABOLISM AND FUNCTION
    Reidar Wallin; Fiscal Year: 2001
    ..The experiments should also contribute to the exciting, rapidly expanding area of research on extra-hepatic vitamin K-dependent proteins. ..
  18. BETA-ADRENERGIC RECEPTOR POLYMORPHISMS AND HYPERTENSION
    Julie Johnson; Fiscal Year: 2003
    ..the BAR genotype significantly impacts the BP response to B-blockers could lead to improved patient outcomes, as patients could be genotyped prior to institution of B-blocker therapy, thus avoiding this therapy in those likely to respond ..
  19. Hypertension Pharmacogenetics
    Julie Johnson; Fiscal Year: 2006
    ..The proposed studies should add substantial new information about antihypertensive pharmacogenetics, and could influence how antihypertensive medications are prescribed in the future. ..
  20. MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONS
    MARTIN E NEWCOMB; Fiscal Year: 2010
    ..A better understanding of the fundamental nature of the reactive species formed in the enzymes and the mechanisms of their reactions is expected to lead to lead ultimately to health-related advances in disease treatment and drug design. ..
  21. Safety of Nicotine Reduction Strategy
    Neal Benowitz; Fiscal Year: 2009
    ..The proposed research is needed to guide governmental regulators as to how best to regulate nicotine in cigarettes and is an essential first step in examining the feasibility of a national regulation program. ..
  22. Active Site Models of CYP4 Enzymes
    ALLAN EDWARD RETTIE; Fiscal Year: 2010
    ..abstract_text> ..
  23. Pharmacogenetics of ADRs: Warfarin Toxicity
    ALLAN EDWARD RETTIE; Fiscal Year: 2010
    ..abstract_text> ..
  24. Warfarin Pharmacogenetics in Pediatric Patients
    RONALD N contact HINES; Fiscal Year: 2010
    ..The IWPC clearly stated that additional research was needed with respect to the development of a similar algorithm for children and young adults. The proposed research will fill this important knowledge gap. ..
  25. NUTRITIONAL COPPER STATUS AND THE NERVOUS SYSTEM
    JOSEPH ROBERT PROHASKA; Fiscal Year: 2010
    ..AIM 4: We will test the hypothesis that limitation in Cu-dependent ferroxidases lead to lower brain iron and are responsible by comparing rats reared on an iron-fortified diet to restore brain iron. ..
  26. HUMAN FMO'S DETERMINANTS OF SUBSTRATE SPECIFICITY
    Allan Rettie; Fiscal Year: 2004
    ..D) Determine the mechanism underlying loss of function in FMO3 variants associated with trimethylaminuria as a further test of the model. (E) Prepare diffraction quality crystals of CMO. ..
  27. REGULATION OF FLAVIN MONOOXYGENASE GENE EXPRESSION
    RONALD HINES; Fiscal Year: 2003
    ..It will be critical for the future rationale design of drugs and therapeutic regimens, as well as for the development of prevention/intervention strategies for at risk individuals or populations. ..
  28. ACTH INDUCTION OF CYTOCHROME P450 IN ADRENAL CELLS
    Michael Waterman; Fiscal Year: 2002
    ..These events are required for the biosynthesis of steroid hormones leading to reproductive capacity and metabolic homeostasis. ..
  29. MECHANISMS OF TOXICITY GORDON CONFERENCE
    RONALD HINES; Fiscal Year: 2002
    ..There also is substantial involvement of new personnel as Chairs and co-Chairs. Thus, the Mechanism of Toxicity GRC is poised to continue its growth as the single leading small conference on Molecular Toxicology. ..
  30. NITROGENASE MECHANISM AND ASSEMBLY
    Thomas Poulos; Fiscal Year: 2002
    ..These studies are expected to have an important impact on our understanding of the general issue of how preformed metal clusters can be inserted into proteins. ..
  31. 7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEIN
    Thomas Poulos; Fiscal Year: 2002
    ..g. superoxide, NO). ..
  32. DNA RADICAL KINETICS
    Martin Newcomb; Fiscal Year: 2001
    ....
  33. Comparison of CYP2 Genes Across Mammal Species
    Susan Hoffman; Fiscal Year: 2004
    ..abstract_text> ..
  34. Biochemistry of Phenazine Production in Pseudomonas aeruginosa
    James Parsons; Fiscal Year: 2009
    ..New strategies that complement existing antimicrobial therapies clearly need to be developed to more effectively combat P. aeruginosa infections. ..
  35. Chromatin Remodeling as a Mechanism to Produce Drug Tolerance
    Nigel Atkinson; Fiscal Year: 2009
    ..We will provide a unified description of how this gene senses and responds to the effects of abused solvent sedation. ..
  36. Protein radicals in O2 reduction by Cytochrome Oxidase
    DENIS PROSHLYAKOV; Fiscal Year: 2008
    ..Application of UV spectroscopy for radical detection will provide a simple and accessible tool for an early detection and kinetic studies of transient radicals in the rapidly growing family of metalloradical enzymes. ..
  37. Nanoscale approaches to allelic silencing in myocardial disease states
    Euan Ashley; Fiscal Year: 2009
    ..This familial condition where the heart grows too thick is the most common cause of sudden death in young people and athletes. ..
  38. CHEMOPREVENTIVE AGENTS AND HUMAN CYTOCHROME P450 IN VIVO
    GRANT WILKINSON; Fiscal Year: 2002
    ..Moreover, the information will be of value in the further development and evaluation of the efficacy/ toxicity of these new chemopreventive agent. ..
  39. MECHANISM OF UREASE METALLOCENTER BIOSYNTHESIS
    Robert P Hausinger; Fiscal Year: 2010
    ..Significantly, our focus on understanding urease activation will uncover common principles that can be applied to these less tractable systems. In addition, we will expand our knowledge regarding the biochemistry of nickel. ..
  40. Gordon Research Conference on Protein-Derived Cofactors
    JUDITH KLINMAN; Fiscal Year: 2002
    ..The vigor of discipline is evidenced by the exponential growth in the papers published in this field, and the students trained during the last ten years. ..
  41. Structure & Function of the Gamma-Glutamyl Carboxylase
    Darrel W Stafford; Fiscal Year: 2011
    ....
  42. FUNCTIONAL CHARACTERIZATION OF AGT GENETIC POLYMORPHISM
    Jun yan Hong; Fiscal Year: 2001
    ..It could have a significant impact on cancer prevention by identifying susceptible subpopulations in future epidemiological studies. ..
  43. The Role of 20-HETE in the Pathogenesis of Stroke
    Samuel Poloyac; Fiscal Year: 2009
    ..Furthermore, these studies will provide important information about the role of monohydroxylated arachidonic acid metabolites as a contributing pathway in the pathogenesis of stroke. ..
  44. Creation of a fatty acid 2-hydoxylase-knockout mouse model
    Hiroko Hama; Fiscal Year: 2008
    ..The Fa2h-knockout mouse will be used in future studies to determine the role of 2- hydroxy fatty acids of GalC/sGalC in myelination and myelin function. [unreadable] [unreadable] [unreadable]..
  45. VITAMIN D P450 FUNCTION--NATURAL & DIRECTED MUTAGENESIS
    John Omdahl; Fiscal Year: 2002
    ....
  46. Enzymatic Mechanisms of Two Pyrimidine Decarboxylases
    Jeffrey Smiley; Fiscal Year: 2003
    ....
  47. SLEEP DEPRIVATION AND 5-HT AUTOREGULATORY PROCESSES
    RUSSELL POLAND; Fiscal Year: 2005
    ..A better understanding of these sleep deprivation-related changes has the potential to advance our knowledge, both in sleep physiology and antidepressant mechanisms of action. ..
  48. IKK Beta In Intestinal Inflammation
    Laurence Egan; Fiscal Year: 2005
    ..abstract_text> ..
  49. Is there a Class IV ribonucleotide reductase?
    Pamela Riggs Gelasco; Fiscal Year: 2003
    ..Observation of an Fe cofactor would make the C. ammoniagenes RNR reaction a model for those in pathogenic bacteria. In either case, the factors that control the cofactor assembly and optimal activity will be investigated. ..
  50. Genomic Analysis of Antibiotic Biosynthesis
    David Sherman; Fiscal Year: 2005
    ..coelicolor genome. ..
  51. P-Glycoprotein and Disposition of Psychostimulants
    JOHN MARKOWITZ; Fiscal Year: 2005
    ....
  52. Altitude-induced hypoxia,IUGR and placental function
    Stacy Zamudio; Fiscal Year: 2006
    ..Such work will aid substantially in elucidating the pathologies that involve placenta hypoxia, e.g., preeclampsia, diabetes and IUGR. ..
  53. FUNCT. CHARACTERIZATION OF HUMAN CYP2A6 GENETIC VARIANTS
    Jun yan Hong; Fiscal Year: 2002
    ....
  54. MECHANISM OF INDUCTION OF OLFACTORY TUMORS BY ALACHLOR
    Mary Beth Genter; Fiscal Year: 2002
    ....
  55. Mechanisms of High Flow Induced Vasculopathy
    Elizabeth Jacobs; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  56. A Behavioral Economic Approach to Smoking Cessation
    Janet Audrain McGovern; Fiscal Year: 2007
    ..g., adolescents, older adults) and to other contexts (e.g., prevention). ..
  57. Mitochondrial Ceramide in Chemotherapy-induced Apoptosis
    Lina M Obeid; Fiscal Year: 2010
    ..These studies will set the stage for pre-clinical development of these molecules and will establish mito-ceramides as potential novel chemotherapeutic agents. ..