oxygenases

Summary

Summary: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.

Top Publications

  1. pmc Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis
    Youfa Cheng
    Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093 0116, USA
    Genes Dev 20:1790-9. 2006
  2. ncbi Strigolactone inhibition of shoot branching
    Victoria Gomez-Roldan
    Universite de Toulouse, UPS, CNRS, Surface Cellulaire et Signalisation chez les Végétaux, 24 Chemin de Borde Rouge, F 31326 Castanet Tolosan, France
    Nature 455:189-94. 2008
  3. ncbi Aromatic hydrocarbon dioxygenases in environmental biotechnology
    D T Gibson
    Department of Microbiology, The Center for Biocatalysis and Bioprocessing, The University of lowa, lowa City, IA 52242, USA
    Curr Opin Biotechnol 11:236-43. 2000
  4. ncbi Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins
    Ian J Clifton
    The Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, Oxon OX1 3TA, UK
    J Inorg Biochem 100:644-69. 2006
  5. pmc Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis
    Youfa Cheng
    Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093 0116, USA
    Plant Cell 19:2430-9. 2007
  6. ncbi Role of the jelly-roll fold in substrate binding by 2-oxoglutarate oxygenases
    Weishen Aik
    Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, United Kingdom
    Curr Opin Struct Biol 22:691-700. 2012
  7. ncbi Structural studies on human 2-oxoglutarate dependent oxygenases
    Michael A McDonough
    Chemistry Research Laboratory and the Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford OX1 3TA, UK
    Curr Opin Struct Biol 20:659-72. 2010
  8. ncbi Non-heme iron oxygenases
    Matthew J Ryle
    Department of Microbiology and Molecular Genetics, 160 Giltner Hall, Michigan State University, East Lansing, Michigan 48824, USA
    Curr Opin Chem Biol 6:193-201. 2002
  9. pmc MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea
    Karim Sorefan
    Department of Biology, University of York, York, YO10 5YW, United Kingdom
    Genes Dev 17:1469-74. 2003
  10. ncbi Filling the gap in vitamin A research. Molecular identification of an enzyme cleaving beta-carotene to retinal
    J von Lintig
    Albert Ludwig Universität Freiburg, Institut für Biologie I, Neurobiologie und Tierphysiologie, D 79104 Freiburg, Germany
    J Biol Chem 275:11915-20. 2000

Research Grants

  1. Electron Transfer in Iron and Copper Proteins
    Jay R Winkler; Fiscal Year: 2013
  2. Regulated Accessibility of a cytokine gene locus.
    Anjana Rao; Fiscal Year: 2013
  3. Biosynthesis of Microbial Isoprenoids
    David E Cane; Fiscal Year: 2013
  4. Blood Pressure Regulation: Novel Roles for the Kidney
    Pablo A Ortiz; Fiscal Year: 2013
  5. HEMOPROTEIN OXIDATION AND HEME CATABOLISM
    Paul R Ortiz de Montellano; Fiscal Year: 2013
  6. 12-HETrE regulation of platelets
    MICHAEL ALLAN HOLINSTAT; Fiscal Year: 2013
  7. HORMONAL REGULATION OF BLOOD PRESSURE
    Michal Laniado Schwartzman; Fiscal Year: 2013
  8. Chemistry and Mechanism of Direct DNA Repair Proteins
    Chuan He; Fiscal Year: 2013
  9. HEME OXYGENASE REGULATION OF EICOSANOID BIOSYNTHESIS
    Nader G Abraham; Fiscal Year: 2013
  10. PEDIATRIC PHARMACOLOGY RESEARCH UNIT
    Mary Lieh Lai; Fiscal Year: 2009

Detail Information

Publications295 found, 100 shown here

  1. pmc Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis
    Youfa Cheng
    Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093 0116, USA
    Genes Dev 20:1790-9. 2006
    ....
  2. ncbi Strigolactone inhibition of shoot branching
    Victoria Gomez-Roldan
    Universite de Toulouse, UPS, CNRS, Surface Cellulaire et Signalisation chez les Végétaux, 24 Chemin de Borde Rouge, F 31326 Castanet Tolosan, France
    Nature 455:189-94. 2008
    ..Furthermore, ccd8 mutants demonstrate the diverse effects of strigolactones in shoot branching, mycorrhizal symbiosis and parasitic weed interaction...
  3. ncbi Aromatic hydrocarbon dioxygenases in environmental biotechnology
    D T Gibson
    Department of Microbiology, The Center for Biocatalysis and Bioprocessing, The University of lowa, lowa City, IA 52242, USA
    Curr Opin Biotechnol 11:236-43. 2000
    Aromatic hydrocarbon dioxygenases belong to a large family of Rieske non-heme iron oxygenases. The dioxygenases have a broad substrate specificity and catalyze enantiospecific reactions with a wide range of substrates...
  4. ncbi Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins
    Ian J Clifton
    The Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, Oxon OX1 3TA, UK
    J Inorg Biochem 100:644-69. 2006
    Mononuclear non-heme ferrous iron dependent oxygenases and oxidases constitute an extended enzyme family that catalyze a wide range of oxidation reactions...
  5. pmc Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis
    Youfa Cheng
    Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093 0116, USA
    Plant Cell 19:2430-9. 2007
    ..Our data demonstrate that auxin synthesized by the YUC flavin monooxygenases is an essential auxin source for Arabidopsis thaliana embryogenesis and postembryonic organ formation...
  6. ncbi Role of the jelly-roll fold in substrate binding by 2-oxoglutarate oxygenases
    Weishen Aik
    Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, United Kingdom
    Curr Opin Struct Biol 22:691-700. 2012
    2-Oxoglutarate (2OG) and ferrous iron dependent oxygenases catalyze two-electron oxidations of a range of small and large molecule substrates, including proteins/peptides/amino acids, nucleic acids/bases, and lipids, as well as natural ..
  7. ncbi Structural studies on human 2-oxoglutarate dependent oxygenases
    Michael A McDonough
    Chemistry Research Laboratory and the Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford OX1 3TA, UK
    Curr Opin Struct Biol 20:659-72. 2010
    2-Oxoglutarate and ferrous iron-dependent oxygenases have emerged as an important family of human enzymes that catalyse hydroxylations and related demethylation reactions...
  8. ncbi Non-heme iron oxygenases
    Matthew J Ryle
    Department of Microbiology and Molecular Genetics, 160 Giltner Hall, Michigan State University, East Lansing, Michigan 48824, USA
    Curr Opin Chem Biol 6:193-201. 2002
    Our understanding of the biological significance and chemical properties of non-heme iron oxygenases has increased dramatically in recent years. New group members have emerged from genome sequences and biochemical analyses...
  9. pmc MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea
    Karim Sorefan
    Department of Biology, University of York, York, YO10 5YW, United Kingdom
    Genes Dev 17:1469-74. 2003
    ..A similar role has been proposed for the pea gene, RMS1. Accordingly, MAX4 and RMS1 were found to encode orthologous, auxin-inducible members of the polyene dioxygenase family...
  10. ncbi Filling the gap in vitamin A research. Molecular identification of an enzyme cleaving beta-carotene to retinal
    J von Lintig
    Albert Ludwig Universität Freiburg, Institut für Biologie I, Neurobiologie und Tierphysiologie, D 79104 Freiburg, Germany
    J Biol Chem 275:11915-20. 2000
    ..The carotene-cleaving enzyme, identified here for the first time on the molecular level, is the basis of the numerous branches of vitamin A action and links plant and animal carotene metabolism...
  11. pmc The copper active site of CBM33 polysaccharide oxygenases
    Glyn R Hemsworth
    Department of Chemistry, University of York, Heslington, York YO10 5DD, United Kingdom
    J Am Chem Soc 135:6069-77. 2013
    The capacity of metal-dependent fungal and bacterial polysaccharide oxygenases, termed GH61 and CBM33, respectively, to potentiate the enzymatic degradation of cellulose opens new possibilities for the conversion of recalcitrant biomass ..
  12. ncbi Genetic analysis of genes controlling natural variation of seed coat and flower colors in soybean
    Kiwoung Yang
    Bio Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon, Chungbuk 363 883, Republic of Korea
    J Hered 101:757-68. 2010
    ....
  13. ncbi Cloning and characterization of two 9-cis-epoxycarotenoid dioxygenase genes, differentially regulated during fruit maturation and under stress conditions, from orange (Citrus sinensis L. Osbeck)
    Maria Jesus Rodrigo
    Instituto de Agroquimica y Tecnologia de Alimentos IATA CSIC, Apartado de Correos 73, 46100 Burjassot, Valencia, Spain
    J Exp Bot 57:633-43. 2006
    ..Based on these results, it is suggested that 9-cis-violaxanthin may be the predominant substrate for NCED in the peel of Citrus fruits, whereas 9'-cis-neoxanthin would be the precursor of ABA in photosynthetic tissues...
  14. ncbi Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy
    Valerie Lefebvre
    Laboratoire de Biologie des Semences, UMR 204 INRA INAPG, Institut Jean Pierre Bourgin, 78026 Versailles Cedex, France
    Plant J 45:309-19. 2006
    ..These demonstrate that ABA synthesized in both the endosperm and the embryo participates in the hormonal balance that controls seed dormancy and germination...
  15. pmc Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis
    Guodong Ren
    State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, China
    Plant Physiol 144:1429-41. 2007
    ..These results collectively indicate that NYE1 plays an important regulatory role in Chl degradation during senescence by modulating pheophorbide a oxygenase activity...
  16. pmc A new classification system for bacterial Rieske non-heme iron aromatic ring-hydroxylating oxygenases
    Ohgew Kweon
    Microbiology Division, National Center for Toxicological Research US FDA, Jefferson, AR 72079, USA
    BMC Biochem 9:11. 2008
    Rieske non-heme iron aromatic ring-hydroxylating oxygenases (RHOs) are multi-component enzyme systems that are remarkably diverse in bacteria isolated from diverse habitats...
  17. pmc Structural consequences of effector protein complex formation in a diiron hydroxylase
    Lucas J Bailey
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 105:19194-8. 2008
    ..This description provides insight into the functional role of effector protein binding in all carboxylate-bridged diiron hydroxylases...
  18. ncbi Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase
    Veerle Rottiers
    Huffington Center on Aging, Department of Molecular and Cellular Biology, Baylor College of Medicine, Room M320, One Baylor Plaza, Houston, Texas 77030, USA
    Dev Cell 10:473-82. 2006
    ..These results imply that C. elegans hormone production has multiple steps and is distributed, and that it may provide one way that tissues register their current physiological state during organismal commitments...
  19. ncbi Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana
    U Oster
    Institute of Botany, University of Munich, Menzinger Str 67, D 80638 Munich, Germany, andInstitute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan
    Plant J 21:305-10. 2000
    ..The recombinant enzyme produced in Escherichia coli catalyses an unusual two-step oxygenase reaction that is the 'missing link' in the chlorophyll cycle of higher plants...
  20. pmc Nitric oxide dioxygenase: an enzymic function for flavohemoglobin
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, and Department of Pediatrics, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:10378-83. 1998
    ....
  21. pmc Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M
    Stephen M Smith
    Departments of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois 60208, United States
    Biochemistry 50:10231-40. 2011
    ..capsulatus (Bath) pMMO. In these new structures, the intramembrane zinc/copper binding site has a different coordination environment from that in previous models...
  22. pmc Analysis of substrate access to active sites in bacterial multicomponent monooxygenase hydroxylases: X-ray crystal structure of xenon-pressurized phenol hydroxylase from Pseudomonas sp. OX1
    Michael S McCormick
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    Biochemistry 50:11058-69. 2011
    ....
  23. pmc Auxin biosynthesis: a simple two-step pathway converts tryptophan to indole-3-acetic acid in plants
    Yunde Zhao
    Section of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0116, USA
    Mol Plant 5:334-8. 2012
    ..The two-step conversion of Trp to IAA is the main auxin biosynthesis pathway that plays an essential role in many developmental processes...
  24. ncbi The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana
    Nicole Schenk
    Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH 3013 Bern, Switzerland
    FEBS Lett 581:5517-25. 2007
    ..From our data we conclude that AtCLHs are not required for senescence-related chlorophyll breakdown in vivo and propose that genuine chlorophyllase has not yet been molecularly identified...
  25. ncbi Practical issues in the application of oxygenases
    Jan B van Beilen
    Institute of Biotechnology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Trends Biotechnol 21:170-7. 2003
    b>Oxygenases carry out the regio-, stereo- and chemoselective introduction of oxygen in a tremendous range of organic molecules. This versatility has already been exploited in several commercial processes...
  26. pmc Biphenyl dioxygenases: functional versatilities and directed evolution
    Kensuke Furukawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812 8581, Japan
    J Bacteriol 186:5189-96. 2004
  27. ncbi Crystal structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath) demonstrating geometrical variability at the dinuclear iron active site
    D A Whittington
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 123:827-38. 2001
    ..Collectively, these results help to explain previous spectroscopic observations and provide new insight into catalysis by the enzyme...
  28. ncbi Recent advances in betalain research
    Dieter Strack
    Abteilung Sekundärstoffwechsel, Leibniz Institut für Pflanzenbiochemie, Weinberg 3, D 06120 Halle Saale, Germany
    Phytochemistry 62:247-69. 2003
    ..Betalains have attracted workers in applied fields because of their use for food colouring and their antioxidant and radical scavenging properties for protection against certain oxidative stress-related disorders...
  29. pmc Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis
    Hanna M Dudek
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    Appl Environ Microbiol 77:5730-8. 2011
    ..These findings will aid in more dedicated and effective redesign of PAMO and related monooxygenases toward an expanded substrate scope...
  30. ncbi Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family
    Bao Cai Tan
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA
    Plant J 35:44-56. 2003
    ..In addition, differential membrane-binding capacity of AtNCEDs is a potential means of post-translational regulation of NCED activity...
  31. ncbi Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)
    Yantao Li
    School of Biological Sciences, University of Hong Kong, Hong Kong, China
    Planta 228:735-43. 2008
    ..Taken altogether, we conclude that in dark-grown Chlorella zofingiensis, the transcription of BKT and CHYb genes are differently regulated by the metabolism of glucose, through which the biosynthesis of astaxanthin is regulated...
  32. ncbi Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli
    Takuya Makino
    School of Fisheries Sciences, Kitasato University, Sanriku cho, Ofunato, 022 0101 Japan
    Plant Cell Physiol 49:1867-78. 2008
    ..PCC 6803, Anabaena sp. PCC 7120 and N. punctiforme PCC 73102, based on the present and previous findings. This explains why these cyanobacteria cannot produce astaxanthin and why only Synechocystis sp. PCC 6803 can produce zeaxanthin...
  33. ncbi Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering
    Tomohisa Hasunuma
    Research institute of Innovative Technology for the Earth RITE, 9 2 Kizugawadai, Kizugawa shi, Kyoto 619 0292, Japan
    Plant J 55:857-68. 2008
    ..The photosynthesis rate of the transformants was also found to be similar to that of wild-type plants under ambient CO2 concentrations of 1500 micromol photons m(-2) s(-1) light intensity...
  34. ncbi Stress-related differential expression of multiple beta-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis
    Jun Chao Huang
    Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
    J Biotechnol 122:176-85. 2006
    ..pluvialis accumulates large amounts of astaxanthin in an instant response to stress environments...
  35. ncbi Regulation of ABA level and water-stress tolerance of Arabidopsis by ectopic expression of a peanut 9-cis-epoxycarotenoid dioxygenase gene
    Xiao Rong Wan
    Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China
    Biochem Biophys Res Commun 347:1030-8. 2006
    ....
  36. ncbi Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase
    B Kauppi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Structure 6:571-86. 1998
    ..The present study was conducted to provide essential structural information necessary for elucidating the mechanism of action of NDO...
  37. pmc The Arabidopsis YUCCA1 flavin monooxygenase functions in the indole-3-pyruvic acid branch of auxin biosynthesis
    Anna N Stepanova
    Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
    Plant Cell 23:3961-73. 2011
    ....
  38. ncbi Arabidopsis thaliana expresses a second functional flavonol synthase
    Anja Preuss
    Philipps Universitat Marburg, Institut fur Pharmazeutische Biologie, Marburg Lahn, Germany
    FEBS Lett 583:1981-6. 2009
    ..These double mutant and biochemical data demonstrate for the first time that LDOX is capable of catalyzing the in planta formation of flavonols...
  39. ncbi A role for flavin monooxygenase-like enzymes in auxin biosynthesis
    Y Zhao
    Howard Hughes Medical Institute, Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Science 291:306-9. 2001
    ..Results from tryptophan analog feeding experiments and biochemical assays indicate that YUCCA catalyzes hydroxylation of the amino group of tryptamine, a rate-limiting step in tryptophan-dependent auxin biosynthesis...
  40. ncbi CMO1 deficiency abolishes vitamin A production from beta-carotene and alters lipid metabolism in mice
    Susanne Hessel
    Institute of Biology I, Animal Physiology and Neurobiology, Hauptstrasse 1, D 79104 Freiburg, Germany
    J Biol Chem 282:33553-61. 2007
    ..These plant-derived compounds must be cleaved and metabolically converted by intrinsic carotenoid oxygenases to support the panoply of vitamin A-dependent physiological processes...
  41. ncbi Regulation of Jumonji-domain-containing histone demethylases by hypoxia-inducible factor (HIF)-1alpha
    Patrick J Pollard
    Henry Wellcome Building for Molecular Physiology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Biochem J 416:387-94. 2008
    ..we have analysed regulation by hypoxia among transcripts encoding human Fe(II)- and 2-oxoglutarate-dependent oxygenases. Our results show that many of these genes are regulated by hypoxia and define two groups of histone ..
  42. ncbi Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds
    Hikaru Suenaga
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Environ Microbiol 9:2289-97. 2007
    ..The metagenomic approach was thus used to retrieve novel EDO genes as well as to gain insights into the gene evolution of EDOs...
  43. pmc Oxidation of benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by toluene 4-monooxygenase of Pseudomonas mendocina KR1 and toluene 3-monooxygenase of Ralstonia pickettii PKO1
    Ying Tao
    Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269 3222, USA
    Appl Environ Microbiol 70:3814-20. 2004
    ..0 +/- 0.8, 4.0 +/- 0.6, and 2.4 +/- 0.3 nmol/min/mg of protein for T4MO, T3MO, and TOM, respectively, at a toluene concentration of 165 microM)...
  44. ncbi SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis
    Lin Chuan Li
    National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China
    New Phytol 179:751-64. 2008
    ....
  45. ncbi Oxygen sensing by HIF hydroxylases
    Christopher J Schofield
    The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, Department of Chemistry, South Parks Road, Oxford OX1 3QY, UK
    Nat Rev Mol Cell Biol 5:343-54. 2004
  46. ncbi Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A
    C Kiefer
    University of Freiburg, Instiute of Biology I, Animal Physiology and Neurobiology, Hauptstrasse 1, D 79104 Freiburg, Germany
    J Biol Chem 276:14110-6. 2001
    ..Thus, in contrast to Drosophila, in vertebrates both symmetric and asymmetric cleavage pathways exist for carotenes, revealing a greater complexity of carotene metabolism...
  47. ncbi The role of active-site residues in naphthalene dioxygenase
    Rebecca E Parales
    Section of Microbiology, 226 Briggs Hall, University of California, Davis, CA 95616, USA
    J Ind Microbiol Biotechnol 30:271-8. 2003
    ..strain NCIB 9816-4. A member of a large family of bacterial Rieske non-heme iron oxygenases, naphthalene dioxygenase is known to oxidize over 60 different aromatic compounds, and many of the products are ..
  48. ncbi The Arabidopsis TDS4 gene encodes leucoanthocyanidin dioxygenase (LDOX) and is essential for proanthocyanidin synthesis and vacuole development
    Sharon Abrahams
    CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, Australia
    Plant J 35:624-36. 2003
    ..These results show that in addition to its established role in the formation of anthocyanin, LDOX is also part of the PA biosynthesis pathway...
  49. pmc Characterization and functional identification of a novel plant 4,5-extradiol dioxygenase involved in betalain pigment biosynthesis in Portulaca grandiflora
    Laurent Christinet
    Laboratory of Plant Cell Genetics, Department of Plant Molecular Biology, University of Lausanne, CH 1015 Lausanne, Switzerland
    Plant Physiol 134:265-74. 2004
    ..These homologs are part of a novel conserved plant gene family probably involved in aromatic compound metabolism...
  50. ncbi Evolution of the soluble diiron monooxygenases
    Joseph G Leahy
    Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    FEMS Microbiol Rev 27:449-79. 2003
    ..likely to have been acquired by horizontal transfer from ancestors common to unrelated diiron and Rieske center oxygenases and other enzymes...
  51. ncbi Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens
    K Saito
    Laboratory of Molecular Biology and Biotechnology, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Plant J 17:181-9. 1999
    ..exhibits significant similarities in amino acid sequence with members of a family of 2-oxoglutarate-dependent oxygenases, no biochemical evidence has been presented which identifies the actual reaction that is catalyzed by ANS...
  52. pmc Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. strain DSM 17874
    Mimmi Throne-Holst
    SINTEF Materials and Chemistry, Department of Biotechnology, Norwegian University of Science and Technology, Sem Saelandsvei 2A, 7465 Trondheim, Norway
    Appl Environ Microbiol 73:3327-32. 2007
    ..Genes encoding AlmA homologues were also identified in other long-chain n-alkane-degrading Acinetobacter strains...
  53. ncbi The X-ray structure of ferric Escherichia coli flavohemoglobin reveals an unexpected geometry of the distal heme pocket
    Andrea Ilari
    CNR Center of Molecular Biology and the Department of Biochemical Sciences, University of La Sapienza, 00185 Rome, Italy
    J Biol Chem 277:23725-32. 2002
    ..Surprisingly, imidazole, cyanide, and azide binding profiles at equilibrium are not accounted for by a single site titration curve but are biphasic and strongly suggest the presence of two distinct conformers within the liganded species...
  54. ncbi Oxygenases: mechanisms and structural motifs for O(2) activation
    T D Bugg
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Curr Opin Chem Biol 5:550-5. 2001
    ..High-valent iron-oxo intermediates have been implicated in heme- and pterin-dependent mono-oxygenases. Structural motifs have been identified for binding of non-heme iron(II) (His,His,Glu) and iron(III) (His(2)Tyr(..
  55. pmc X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. OX1 phenol hydroxylase
    Matthew H Sazinsky
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 45:15392-404. 2006
    ..Comparisons between the ToMOH and PHH structures provide insights into their substrate regiospecificities...
  56. ncbi Functional and evolutionary relationships among diverse oxygenases
    S Harayama
    Department of Medical Biochemistry, University of Geneva, Switzerland
    Annu Rev Microbiol 46:565-601. 1992
    b>Oxygenases that incorporate one or two atoms of dioxygen into substrates are found in many metabolic pathways...
  57. pmc Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a
    A Tanaka
    The Institute of Low Temperature Science, Hokkaido University, Sapporo 0600819, Japan
    Proc Natl Acad Sci U S A 95:12719-23. 1998
    ..The results demonstrate that a chlorophyll a oxygenase is involved in chlorophyll b formation. The reaction mechanism of chlorophyll b formation is discussed...
  58. pmc Mammalian carotenoid-oxygenases: key players for carotenoid function and homeostasis
    Glenn P Lobo
    Case Western Reserve University, School of Medicine, Department of Pharmacology, Cleveland, OH 44106, USA
    Biochim Biophys Acta 1821:78-87. 2012
    ..for carotenoid metabolism have been identified, including an evolutionarily well conserved family of carotenoid-oxygenases. Studies in knockout mouse models for these enzymes revealed that carotenoid metabolism is a highly regulated ..
  59. pmc Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity
    M Petrusma
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Appl Environ Microbiol 75:5300-7. 2009
    ..tuberculosis enzyme may provide a novel target for treatment of tuberculosis. Detailed knowledge about the biochemical properties of KSH thus is highly relevant in the research fields of biotechnology and medicine...
  60. ncbi Differential developmental and tissue-specific regulation of expression of the genes encoding three members of the flavin-containing monooxygenase family of man, FMO1, FMO3 and FM04
    C T Dolphin
    Department of Biochemistry, Queen Mary and Westfield College, University of London, UK
    Eur J Biochem 235:683-9. 1996
    ..FMO4 mRNA is present in low abundance in several foetal and adult tissues and thus the corresponding gene appears to be expressed constitutively...
  61. pmc A novel testosterone catabolic pathway in bacteria
    Yann Lii Leu
    Natural Products Laboratory, Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, Taiwan
    J Bacteriol 193:4447-55. 2011
    ..To our knowledge, an enzymatic hydration reaction occurring at the A ring of steroid compounds has not been reported before...
  62. ncbi Cloning and sequencing of flavin-containing monooxygenases FMO3 and FMO4 from rabbit and characterization of FMO3
    V L Burnett
    Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 269:14314-22. 1994
    ..Rabbit genomic DNA was probed under conditions of low stringency with a mixture of 5' cDNA fragments encoding all five FMO forms and produced results consistent with the possibility of one additional FMO...
  63. pmc EcdGHK are three tailoring iron oxygenases for amino acid building blocks of the echinocandin scaffold
    Wei Jiang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Chem Soc 135:4457-66. 2013
    ....
  64. ncbi An insight into the origin and functional evolution of bacterial aromatic ring-hydroxylating oxygenases
    Joydeep Chakraborty
    Department of Microbiology, Bose Institute, Kolkata, 700 054, India
    J Biomol Struct Dyn 30:419-36. 2012
    Bacterial aromatic ring-hydroxylating oxygenases (RHOs) are multicomponent enzyme systems which have potential utility in bioremediation of aromatic compounds in the environment...
  65. pmc Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b
    N Elango
    Department of Biochemistry, Medical School, University of Minnesota, Minneapolis 55455, USA
    Protein Sci 6:556-68. 1997
    ..Fifteen additional sequence differences distributed randomly over the three chains were also observed, including D alpha 209E, a ligand of one of the irons...
  66. pmc Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400
    B D Erickson
    Bioremediation Laboratory, General Electric Co, Schenectady, New York 12301
    J Bacteriol 174:2903-12. 1992
    ..Transcription initiating at the site furthest upstream is greatly increased when the LB400 cells are grown on biphenyl as the sole carbon source...
  67. pmc Versatility of biological non-heme Fe(II) centers in oxygen activation reactions
    Elena G Kovaleva
    Department of Biochemistry, Molecular Biology and Biophysics and the Center for Metals in Biocatalysis, University of Minnesota, 6 155 Jackson Hall, Minneapolis, Minnesota 55455, USA
    Nat Chem Biol 4:186-93. 2008
    ..The reactive species formed in these reactions have been proposed to encompass four oxidation states of iron and all forms of reduced O2 as well as several of the reactive oxygen species that derive from O-O bond cleavage...
  68. pmc Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases
    E L Neidle
    Department of Biology, Yale University, New Haven, Connecticut 06511
    J Bacteriol 173:5385-95. 1991
    ..Regions of possible NAD and flavin adenine dinucleotide binding were identified...
  69. ncbi Carotenoid oxygenases: cleave it or leave it
    Giovanni Giuliano
    ENEA, Casaccia Research Centre, Roma, Italy
    Trends Plant Sci 8:145-9. 2003
    ..The first step in their biosynthesis is the oxidative cleavage of a carotenoid catalysed by a non-heme iron oxygenase. A novel family of enzymes, which can cleave different carotenoids at different positions, has been characterized...
  70. ncbi Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase
    Ralf Stracke
    Genome Research, Bielefeld University, 33594, Bielefeld, Germany
    Planta 229:427-45. 2009
    ..By using a leucoanthocyanidin dioxygenase (ldox) fls1-2 double mutant, we present evidence that the remaining flavonol glycosides found in the fls1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme...
  71. pmc Two structures of an N-hydroxylating flavoprotein monooxygenase: ornithine hydroxylase from Pseudomonas aeruginosa
    Jose Olucha
    Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 286:31789-98. 2011
    ....
  72. pmc The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean
    X Qin
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 96:15354-61. 1999
    ..The results provide evidence that drought-induced ABA biosynthesis is regulated by the 9-cis-epoxycarotenoid cleavage reaction and that this reaction takes place in the thylakoids, where the carotenoid substrate is located...
  73. pmc The membrane-associated form of methane mono-oxygenase from Methylococcus capsulatus (Bath) is a copper/iron protein
    Piku Basu
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U K
    Biochem J 369:417-27. 2003
    ..The EPR measurements also demonstrated the presence of a tightly bound mononuclear Fe(3+) ion in an octahedral environment that may well be exchange-coupled to another paramagnetic species...
  74. ncbi Dynamical regulation of ligand migration by a gate-opening molecular switch in truncated hemoglobin-N from Mycobacterium tuberculosis
    Axel Bidon-Chanal
    Departament de Fisicoquimica, Facultat de Farmacia, Universitat de Barcelona, Avenida Diagonal 643, 08028, Barcelona, Spain
    J Am Chem Soc 129:6782-8. 2007
    ..The results support a molecular mechanism evolved to ensure access of NO to the heme cavity in the oxygenated form of the protein, which should warrant survival of the microorganism under stress conditions...
  75. ncbi Quantum mechanical/molecular mechanical study on the mechanism of the enzymatic Baeyer-Villiger reaction
    Iakov Polyak
    Max Planck Institut fur Kohlenforschung, Kaiser Wilhelm Platz 1, D 45470 Mulheim an der Ruhr, Germany
    J Am Chem Soc 134:2732-41. 2012
    ....
  76. pmc Ruffling of metalloporphyrins bound to IsdG and IsdI, two heme-degrading enzymes in Staphylococcus aureus
    Woo Cheol Lee
    Department of Microbiology and Immunology, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 283:30957-63. 2008
    ..belong to a newly identified heme degradation family distinct from canonical eukaryotic and prokaryotic heme oxygenases. Here we report the crystal structures of an inactive N7A variant of IsdG in complex with Fe(3+)-protoporphyrin ..
  77. ncbi A carotenoid synthesis gene cluster from a non-marine Brevundimonas that synthesizes hydroxylated astaxanthin
    Luan Tao
    Biological and Chemical Sciences and Engineering, Central Research and Development, EI DuPont de Nemours Inc, Wilmington, DE 19880 0328, USA
    Gene 379:101-8. 2006
    ..The main difference was that the CrtG from B. aurantiaca worked well on canthaxanthin or astaxanthin, but the CrtG from DC263 did not work on either of the ketocarotenoids...
  78. ncbi Potential roles for regulatory oxygenases in rickettsial pathogenesis
    Sanjeev K Sahni
    Hematology Oncology Unit, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Ann N Y Acad Sci 1063:207-14. 2005
    ....
  79. ncbi Identification, expression, and substrate specificity of a mammalian beta-carotene 15,15'-dioxygenase
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:6560-5. 2001
    ..Identification of beta-CD in an accessible model organism will create new opportunities to study vitamin A metabolism...
  80. ncbi Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin
    Sabine Steiger
    Biosynthesis Group, Botanical Institute, Goethe University, P O Box 111932, D 60054 Frankfurt, Germany
    Biotechnol Lett 26:813-7. 2004
    ..The gene product of crtW38 was unable to catalyze this reaction. Both ketolases differ in their interaction with a hydroxylase in the biosynthetic pathway from beta-carotene to astaxanthin...
  81. ncbi The cyanobacterium Anabaena sp. PCC 7120 has two distinct beta-carotene ketolases: CrtO for echinenone and CrtW for ketomyxol synthesis
    Mari Mochimaru
    Natural Science Faculty, Komazawa University, Tokyo 154 8525, Japan
    FEBS Lett 579:6111-4. 2005
    ..This cyanobacterium was the first species found to have both enzymes, which functioned in two distinct biosynthetic pathways...
  82. ncbi Characterization of two beta-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
    Seon Kang Choi
    Marine Biotechnology Institute, Heita, Kamaishi shi, Iwate 026 0001, Japan
    Appl Microbiol Biotechnol 75:1335-41. 2007
    ....
  83. ncbi Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad
    S Han
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Science 270:976-80. 1995
    ..The iron atom has five ligands in square pyramidal geometry: one glutamate and two histidine side chains, and two water molecules...
  84. ncbi Rieske business: structure-function of Rieske non-heme oxygenases
    Daniel J Ferraro
    Department of Biochemistry, University of Iowa Roy J and Lucille A Carver College of Medicine, 51 Newton Road, 4 403 BSB, Iowa City, IA 52242, USA
    Biochem Biophys Res Commun 338:175-90. 2005
    Rieske non-heme iron oxygenases (RO) catalyze stereo- and regiospecific reactions. Recently, an explosion of structural information on this class of enzymes has occurred in the literature...
  85. pmc Mutation in bovine beta-carotene oxygenase 2 affects milk color
    S D Berry
    ViaLactia Biosciences, Newmarket, Auckland, New Zealand
    Genetics 182:923-6. 2009
    ..The BCO2 enzyme is thereby identified as a key regulator of beta-carotene metabolism...
  86. ncbi Crystal structure of naphthalene dioxygenase: side-on binding of dioxygen to iron
    Andreas Karlsson
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, Biomedical Center, 75124 Uppsala, Sweden
    Science 299:1039-42. 2003
    ..The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase...
  87. ncbi Towards a better understanding of carotenoid metabolism in animals
    Johannes von Lintig
    Institute of Biology I, Dept of Animal Physiology and Neurobiology, University of Freiburg, Hauptstrasse 1, D 79104 Freiburg, Germany
    Biochim Biophys Acta 1740:122-31. 2005
    ..This review will summarize the advanced state of knowledge about the vitamin A biosynthetic pathway and will discuss biochemical, physiological, developmental and medical aspects of carotenoids and their numerous derivatives...
  88. ncbi Expanding chemical biology of 2-oxoglutarate oxygenases
    Christoph Loenarz
    Chemistry Research Laboratory and the Oxford Centre for Integrative Systems Biology, Mansfield Road, Oxford OX1 3TA, UK
    Nat Chem Biol 4:152-6. 2008
    ..signaling and fatty acid metabolism, recent reports have now revealed roles for human 2-oxoglutarate-dependent oxygenases in histone and nucleic acid demethylation and in signaling protein hydroxylation...
  89. ncbi Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane
    A C Rosenzweig
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139
    Nature 366:537-43. 1993
    ..An extended canyon runs between alpha beta pairs, which have many long alpha-helices, for possible docking of the reductase and coupling proteins required for catalysis...
  90. pmc The membrane-associated methane monooxygenase (pMMO) and pMMO-NADH:quinone oxidoreductase complex from Methylococcus capsulatus Bath
    Dong W Choi
    Department of Biochemistry, Biophysics, and Molecular Biology, and Graduate Program in Microbiology, Iowa State University, Ames, Iowa 50011 3211, USA
    J Bacteriol 185:5755-64. 2003
    ..capsulatus Bath, along with NADH and duroquinol, to enzyme assays increased the activity of purified preparations. The NDH and NADH were added to maintain a high duroquinol/duroquinone ratio...
  91. pmc Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize
    Changfu Zhu
    Departament de Produccio Vegetal i Ciencia Forestal, Universitat de Lleida, Avenue Alcalde Rovira Roure, 191, 25198 Lleida, Spain
    Proc Natl Acad Sci U S A 105:18232-7. 2008
    ..Combinatorial transformation is a versatile approach that could be used to modify any metabolic pathway and pathways controlling other biochemical, physiological, or developmental processes...
  92. pmc Hydroquinone dioxygenase from pseudomonas fluorescens ACB: a novel member of the family of nonheme-iron(II)-dependent dioxygenases
    Mariëlle J H Moonen
    Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands
    J Bacteriol 190:5199-209. 2008
    ..HQDO is a novel member of the family of nonheme-iron(II)-dependent dioxygenases. The enzyme shows insignificant sequence identity with known dioxygenases...
  93. pmc The metal centers of particulate methane monooxygenase from Methylosinus trichosporium OB3b
    Amanda S Hakemian
    Department of Biochemistry, Northwestern University, Evanston, Illinois 60208, USA
    Biochemistry 47:6793-801. 2008
    ..trichosporium OB3b pMMO. These findings extend previous work on pMMO from M. capsulatus (Bath) and provide new insight into the functional importance of the different metal centers...
  94. ncbi MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone
    Jonathan Booker
    Department of Biology, Area 11, University of York, York, YO10 5YW, United Kingdom
    Dev Cell 8:443-9. 2005
    ..Moreover, the proposed enzymatic series for MAX hormone synthesis resembles that of two already characterized signal biosynthetic pathways: prostaglandins in animals and oxilipins in plants...
  95. pmc Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin
    Rick W Ye
    DuPont Experimental Station, Route 141 and Henry Clay Road, Wilmington, DE 19880, USA
    Appl Environ Microbiol 72:5829-37. 2006
    ..Three randomly generated mutants, carrying L175M, M99V, and M99I, were identified to have improved activity. These mutants are potentially useful in pathway engineering for the production of astaxanthin...
  96. ncbi Nitric oxide scavenging and detoxification by the Mycobacterium tuberculosis haemoglobin, HbN in Escherichia coli
    Ranjana Pathania
    Institute of Microbial Technology, Sector 39A, Chandigarh, 160 036 India
    Mol Microbiol 45:1303-14. 2002
    ..tuberculosis HbN. The catalytic NO scavenging by HbN may be highly advantageous for the survival of tubercle bacilli during infection and pathogenesis...
  97. ncbi Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1
    Yutaka Furusawa
    Biological Information Research Center BIRC, National Institute of Advanced Industrial Science and Technology AIST, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
    J Mol Biol 342:1041-52. 2004
    ..The cooperative actions of residues in the three groups seem to determine the substrate specificity of the enzyme...
  98. pmc Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR
    Brett R Baldwin
    School of Civil Engineering, Purdue University, West Lafayette, Indiana 47907 2051, USA
    Appl Environ Microbiol 69:3350-8. 2003
    ..PCR primer sets were identified for the large subunits of aromatic oxygenases from alignments of known gene sequences and tested with genetically well-characterized strains...
  99. pmc The IsdG-family of haem oxygenases degrades haem to a novel chromophore
    Michelle L Reniere
    Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
    Mol Microbiol 75:1529-38. 2010
    Enzymatic haem catabolism by haem oxygenases is conserved from bacteria to humans and proceeds through a common mechanism leading to the formation of iron, carbon monoxide and biliverdin...
  100. ncbi Crystal structures of cyclohexanone monooxygenase reveal complex domain movements and a sliding cofactor
    I Ahmad Mirza
    Department of Biochemistry, McGill University, 3649 Prom Sir William Osler, Bellini Pavilion, Room 466, Montreal, QC, Canada H3G 0B1
    J Am Chem Soc 131:8848-54. 2009
    ..Finally, the previously identified BVMO signature sequence is highlighted for its role in coordinating domain movements. Taken together, these structures provide mechanistic insights into CHMO-catalyzed Baeyer-Villiger oxidation...
  101. pmc CD and MCD of CytC3 and taurine dioxygenase: role of the facial triad in alpha-KG-dependent oxygenases
    Michael L Neidig
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 129:14224-31. 2007
    The alpha-ketoglutarate (alpha-KG)-dependent oxygenases are a large and diverse class of mononuclear non-heme iron enzymes that require FeII, alpha-KG, and dioxygen for catalysis with the alpha-KG cosubstrate supplying the additional ..

Research Grants62

  1. Electron Transfer in Iron and Copper Proteins
    Jay R Winkler; Fiscal Year: 2013
    ..Important members of this class of enzymes are the heme-thiolate mono- oxygenases such as cytochrome P450 and nitric oxide synthases (NOS)...
  2. Regulated Accessibility of a cytokine gene locus.
    Anjana Rao; Fiscal Year: 2013
    ..that the TET proteins TET1, TET2 and TET3 constitute a new family of 2-oxoglutarate (2OG)- and Fe(II)-dependent oxygenases, that convert 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) in DNA...
  3. Biosynthesis of Microbial Isoprenoids
    David E Cane; Fiscal Year: 2013
    ..2) A variety of catalytically novel oxygenases will be investigated that play key roles in the metabolic conversion of terpene hydrocarbons and alcohols to ..
  4. Blood Pressure Regulation: Novel Roles for the Kidney
    Pablo A Ortiz; Fiscal Year: 2013
    ..Project 3 will test whether heme oxygenases in the macula densa produce carbon monoxide (CO) and biliverdin, which act synergistically and in an autocrine ..
  5. HEMOPROTEIN OXIDATION AND HEME CATABOLISM
    Paul R Ortiz de Montellano; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Heme oxygenases (HOs) oxidize heme to biliverdin, iron, and carbon monoxide (CO) via a complex, three-stage process that requires seven electrons and three molecules of O2...
  6. 12-HETrE regulation of platelets
    MICHAEL ALLAN HOLINSTAT; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Formation of bioactive lipids by oxygenases is known to play both a protective and pro-thrombotic role in circulation...
  7. HORMONAL REGULATION OF BLOOD PRESSURE
    Michal Laniado Schwartzman; Fiscal Year: 2013
    ..EETs and 20-HETE, and two distinct regulatory circuits, the renin-angiotensin system (RAS) and the heme oxygenases (HO). This theme is depicted in three projects...
  8. Chemistry and Mechanism of Direct DNA Repair Proteins
    Chuan He; Fiscal Year: 2013
    ..oxidative repair intermediates which should give clear mechanistic pictures for these as well as related oxygenases. We also propose to characterize protein-DNA interactions in the absence of DNA damage, thus providing further ..
  9. HEME OXYGENASE REGULATION OF EICOSANOID BIOSYNTHESIS
    Nader G Abraham; Fiscal Year: 2013
    ..Previous work has identified the heme oxygenases (HO-1/HO-2), EETs and adiponectin as key endothelial protective molecules...
  10. PEDIATRIC PHARMACOLOGY RESEARCH UNIT
    Mary Lieh Lai; Fiscal Year: 2009
    ..The demonstration of the role of NFKb in the gene expression of inflammatory mediators including the cyclo-oxygenases identified a target focus for drug intervention...
  11. Studies of redox-active sites in Photosystem II
    Victor S Batista; Fiscal Year: 2010
    ..g., P-450s, peroxidases and oxygenases) common to different classes of organisms from bacteria to humans, including drug targets in cancer, antiviral, ..
  12. Regulation of P450 Activity by Cytochrome P450 Oxidoreductase
    JUNG JA P KIM; Fiscal Year: 2013
    ..single CYPOR protein is capable of interacting with ~50 microsomal P450s, as well as the non-P450 enzymes, heme oxygenases (HO-1 and HO-2)...
  13. Transient catalytic oxygen species in iron enzymes
    Denis A Proshlyakov; Fiscal Year: 2013
    ..We will investigate three representative enzymes: ribonucleotide reductase is involved in DNA replication and two bacterial oxygenases provide important models of analogous enzymes in mammals.
  14. Overcoming O2 limitations: Reaction of Oxygenases with in situ generated O2
    BENNETT RICHARD STREIT; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): With non-heme Fe oxidases and oxygenases carrying out some fundamentally very significant biological reactions, gaining an understanding of how they work is vital to being able to implement an ..
  15. Structure and Function of JmjC Histone Demethylases
    Gongyi Zhang; Fiscal Year: 2010
    ..structural determinants for cofactors such as Fe(II) and a-ketoglutarate, a hallmark of non-heme containing oxygenases. There, however, are major questions that remained to be answered, such as the structural basis of the ..
  16. Spectroscopic and Computational Investigation of Copper Monooxygenases
    RYAN COWLEY; Fiscal Year: 2013
    ..catalysis and provide a rationale for active site structural elements in the non-coupled binuclear copper oxygenases. Combined with previous results in the coupled binuclear family, an electronic structure/function model will be ..
  17. Carotinoids in Vision
    JOHANNES FRIEDERICH VONLINTIG; Fiscal Year: 2013
    ..Due to structural conservation of carotenoid-oxygenases and RPE65, comparisons of the substrate binding regions provide an opportunity to indentify amino acid residues ..
  18. Roles of protein structure and diiron cluster chemistry in oxygen activation
    John D Lipscomb; Fiscal Year: 2013
    ..structure in O2 activation, and to further explore the range of chemistry accessible by the diiron cluster oxygenases, new classes of diiron oxygenases are needed that: (i) present the diiron core in a different protein ..
  19. Mechanisms of nonribosomal peptide natural product biosynthesis
    Steven D Bruner; Fiscal Year: 2013
    ..DpgC is a member of a very small group of cofactor/metal independent oxygenases and has unique chemistry and structure...
  20. Mechanistic Insights into TET-Mediated Demethylation in Leukemia
    Louis C Dore; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Recently, mutations in the TET family of oxygenases have been described in a subset of acute myeloid leukemias (AML)...
  21. Heme Oxygenase 2005 -- the 4th International Conference
    FRITZ BACH; Fiscal Year: 2005
    ..and discuss the most recent information on heme oxygenase and the products of the degradation of heme by heme oxygenases: carbon monoxide (CO), Fe++ and biliverdin...
  22. Omega-3 PUFA-gene interaction in prostate cancer
    Yong Q Chen; Fiscal Year: 2013
    ..To systematically assess the interaction between oxygenases and PUFAs in PCa, we knocked out Cox1, Cox2, Lox5, Lox12, and Lox15 in prostate-specific Pten-null mice...
  23. 6th International Conference on Heme Oxygenases
    Anupam Agarwal; Fiscal Year: 2009
    ..provided by applicant): This is a request for partial funding of the 6th International Conference on Heme Oxygenases "Heme Oxygenases in Biology and Medicine" scheduled for September 30th to October 4th, 2009 at Miami Beach, ..
  24. Direct single nucleotide mapping of genomic CpG marks
    Saulius Klimasauskas; Fiscal Year: 2013
    ..substantial fraction of 5mC in CpG dinucleotides is converted to 5-hydroxymethylcytosine (hmC) by the action of oxygenases. These findings provided important clues in a long elusive mechanism of active DNA demethylation and bolstered a ..
  25. PROSTANOIDS IN COLON CANCER--CELL/MOLECULAR MECHANISMS
    James Roche; Fiscal Year: 1999
    ..can be channelled through several enzymatic pathways, including prostaglandin endoperoxide synthases (cyclo-oxygenases) to yield ultimately the prostanoids...
  26. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2004
    ..Heme oxygenases are the only enzymes that metabolize the pro-oxidant heme to bile pigments, iron, and carbon monoxide...
  27. RAPID KINETIC AND OTHER STUDIES OF BIOLOGICAL OXYGENASES
    David Ballou; Fiscal Year: 2002
    This project is a multi-disciplinary study of the structure and function of oxygenases and other redox enzymes. Our goal is to understand the chemical mechanism whereby oxygen is activated by oxygenases...
  28. Nitroxyl adducts as structural probes of oxygenase/substrate interactions
    Thomas Poulos; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): The structures of heme-based oxygenases (e.g...
  29. INSECT HEME OXYGENASE
    Boris Dunkov; Fiscal Year: 2004
    ..of the mammalian and algal heme oxygenase systems, practically no attention has been given to the heme oxygenases in insects. I proposed to study for the first time heme oxygenase in an invertebrate animal...
  30. Understanding coupled transfers of electrons and protons relevant to biological c
    James Mayer; Fiscal Year: 2007
    ..is a key step in the catalytic cycles of many metalloenzymes, including cytochrome P450, various non-heme iron oxygenases, and vitamin B12-dependent enzymes...
  31. NONHEME IRON DIOXYGEN ACTIVATION IN ENZYMES AND MODELS
    Lawrence Que; Fiscal Year: 2009
    ..thiol for isopenicillin N synthase, and the alpha-keto acid co-substrate for alphaketoglutarate- dependent oxygenases. Several of these enzymes have now been shown by crystallography to have an iron center with a common 2- His-1-..
  32. Structure Function Studies on Non-Heme Iron Oxygenases
    Ramaswamy Subramanian; Fiscal Year: 2006
    ..This reaction is catalyzed by the multi-component Rieske non-heme iron oxygenases, which are the focus of this proposal...
  33. ALCOHOL METABOLISM--ROLE OF P450 OXYGENASES
    Minor Coon; Fiscal Year: 2000
    ..Our goals are as follows: 1. To determine the role of alcohol-inducible P450 oxygenases (rabbit liver microsomal isozymes 2E1 and 2E2) in the oxidative cleavage of esters and amides, with the eventual ..
  34. CYTOCHROME P450 ACTIVE OXYGEN STRUCTURE AND MECHANISMS
    John Dawson; Fiscal Year: 2000
    DESCRIPTION: Understanding dioxygen activation by the cytochrome P450 mono-oxygenases requires knowledge of structure at the molecular level...
  35. SYNTHESES OF HEMES FOR PROTEIN STUDIES
    Kevin Smith; Fiscal Year: 2003
    ..relationships in a variety of biologically important heme proteins such as hemoglobins, myoglobins, cytochromes, oxygenases, and peroxidases...
  36. SYNTHESES OF HEMES FOR PROTEIN STUDIES
    Kevin Smith; Fiscal Year: 2002
    ..relationships in a variety of biologically important heme proteins such as hemoglobins, myoglobins, cytochromes, oxygenases, and peroxidases...
  37. NON-HEME IRON OXYGEN ACTIVATION IN ENZYME AND MODELS
    Lawrence Que; Fiscal Year: 1999
    ..and the a-keto acid cofactor for prolyl hydroxylase, clavarninate synthase and other a-ketoglutarate-dependent oxygenases. It is proposed that a common general mechanism unites the chemistry of these enzymes, but the details of the ..
  38. RAPID KINETIC AND OTHER STUDIES OF BIOLOGICAL OXYGENASES
    David Ballou; Fiscal Year: 2009
    Plan and Goals: The long-term objective of the proposed research is to discover how iron in Rieske non-heme iron oxygenases and in cytochromes P450 oxygenases activates oxygen for reactions with substrates...
  39. Regulation of Renal Oxygenation and Blood Pressure
    Pin Lan Li; Fiscal Year: 2006
    ..that the activities of many paracrine or autocrine systems such as nitric oxide synthases, cyclooxyganses, heme oxygenases and others are much higher in the renal medulla than in the renal cortex...
  40. PROSTAGLANDIN SYNTHASE--STRUCTURE AND FUNCTION
    RICHARD KULMACZ; Fiscal Year: 1990
    ..These characteristics are shared by other fatty acid oxygenases and may be important in regulation of eicosanoid biosynthesis...
  41. CHEMISTRY AND BIOLOGY OF NON-HEME IRON OXYGENASES
    Ellen Neidle; Fiscal Year: 2002
    ..A catalytic mechanism for these enzymes and several tests of the mechanism are also proposed. ..
  42. GENERATION, TRANSPORT AND EFFECTS OF EICOSANOIDS
    EDWARD GOETZL; Fiscal Year: 1993
    ..intermediates, lipoxygenase activation and secretion, and natural and pharmacological inhibition of the oxygenases. Similarly, cellular transport proteins and receptors for some eicosanoids have been identified and ..
  43. Oxidative Modifications in Atherogenesis
    Garret FitzGerald; Fiscal Year: 2006
    ..the covalent modifications to DNA resulting from hydroperoxide derived electrophiles; investigate the role of oxygenases in this process; determine from studies in Projects 1 and 5 whether vitamin C administration results in such DNA ..
  44. PGE2 in the Pathogenesis of Allergic Airway Disease
    Beverly Koller; Fiscal Year: 2009
    ..PGE2 is synthesized from arachidonic acid by the sequential actions of phospholipases, cyclo-oxygenases, and PGE synthases. To date, 3 putative PGE synthases have been identified that generate PGE2 from endoperoxides...
  45. OXYGENASE ENZYME MECHANISM
    John Lipscomb; Fiscal Year: 2003
    ..This work should yield fundamental information about the chemistry of oxygenases, O2, and iron.
  46. 600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER
    Daniel Sem; Fiscal Year: 2005
    ..cytochrome P450 oxidoreductase (Kim) and (6) Substrate binding geometries in Fell dependent non-heme oxygenases from paramagnetic relaxation and heteronudear correlation (Moran)...
  47. Molecular Assessment of Hydrocarbon Degradation
    Gerben Zylstra; Fiscal Year: 2004
    ..goal of this project is to obtain information about community function by focusing on the critical initial oxygenases that are the rate-limiting step in productive degradation and eventual mineralization of aromatic compounds in ..
  48. METAL-DIOXYGEN CHEMISTRY OF BIOLOGICAL INTEREST
    William Armstrong; Fiscal Year: 1991
    ..and tetradentate ligands; (4) use of these ligands to prepare iron complexes of potential relevance to non-heme oxygenases and examination of their oxygen chemistry; (5) synthesis of peroxide-bridged dimanganese complexes with bulky ..
  49. EICOSANOIDS BLOOD VESSELS AND HYPERTENSION
    Patrick Wong; Fiscal Year: 1993
    ..Little is known about the mechanism and modulation of the secondary metabolism of EETs by other oxygenases and the possible conjugation with glutathione...
  50. Proposal for Central California 900 MHZ NMR Spectrometer
    David Wemmer; Fiscal Year: 2007
    ..will include large viral and ribosomal RNAs, VS ribozyme, viral protein-RNA interactions, metalloprotein heine oxygenases, TNF ligand and receptor interactions, bacterial transcriptional activators, proteins studied inside cells, ..
  51. Amine N-Oxygenation by FMO3 and FMO4
    John Cashman; Fiscal Year: 2005
    ..Such fundamental information will be useful in the development of safer drugs, the prevention of adverse drug reactions and the protection of humans from disease. ..
  52. New Opioids for Alcoholism
    John Cashman; Fiscal Year: 2006
    ..The potential commercial application of the work is that the research could lead to a 'blockbuster' drug product. [unreadable] [unreadable]..
  53. COMBINATORIAL PHARMACOTHERAPIES FOR COCAINE DEPENDENCE
    John Cashman; Fiscal Year: 2001
    ..The study will provide an understanding of the structural and pharmaceutical properties of hDAT antagonists. The work will lead to new insight into the preparation of human medications necessary in the cessation of cocaine abuse. ..
  54. MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONS
    MARTIN E NEWCOMB; Fiscal Year: 2010
    ..A better understanding of the fundamental nature of the reactive species formed in the enzymes and the mechanisms of their reactions is expected to lead to lead ultimately to health-related advances in disease treatment and drug design. ..
  55. DNA RADICAL KINETICS
    Martin Newcomb; Fiscal Year: 2001
    ....
  56. 20-HETE IN NO-MEDIATED REGULATION OF RENAL FUNCTION
    Adebayo Oyekan; Fiscal Year: 2001
    ....