glyceraldehyde 3 phosphate dehydrogenases

Summary

Summary: Enzymes that catalyze the dehydrogenation of GLYCERALDEHYDE 3-PHOSPHATE. Several types of glyceraldehyde-3-phosphate-dehydrogenase exist including phosphorylating and non-phosphorylating varieties and ones that transfer hydrogen to NADP and ones that transfer hydrogen to NAD.

Top Publications

  1. pmc Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
    Nicholas Silver
    Molecular Haematology, Division of Gene and Cell Based Therapy, King s College London School of Medicine at King s College Hospital, Denmark Hill, London, SE5 9PJ, UK
    BMC Mol Biol 7:33. 2006
  2. ncbi Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    T D Schmittgen
    Department of Pharmaceutical Sciences, College of Pharmacy, and the Cancer Prevention and Research Center, Washington State University, Pullman, WA 99164 6534, USA
    J Biochem Biophys Methods 46:69-81. 2000
  3. ncbi Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:54046-52. 2004
  4. ncbi Differential expression of GAPDH and beta3-actin in growing collateral arteries
    Elisabeth Deindl
    Max Planck Institute, Department of Experimental Cardiology, Bad Nauheim, Germany
    Mol Cell Biochem 236:139-46. 2002
  5. ncbi The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
    K Dheda
    Centre for Infectious Diseases and International Health, Royal Free and University College Medical School, and Department of Thoracic and HIV Medicine, Royal Free Hospital, London NW3 2QG, UK
    Anal Biochem 344:141-3. 2005
  6. ncbi Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications
    Simone Holtgrefe
    Department of Plant Physiology, University of Osnabrueck, D 49069 Osnabrueck, Germany
    Physiol Plant 133:211-28. 2008
  7. ncbi GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues
    Robert D Barber
    Pharmagene Laboratories Limited, Royston, Hertfordshire, United Kingdom
    Physiol Genomics 21:389-95. 2005
  8. ncbi Validation of reference genes for transcription profiling in the salmon louse, Lepeophtheirus salmonis, by quantitative real-time PCR
    Petter Frost
    Department of Aquaculture, Institute of Marine Research, P O Box 1870, Nordnes, N 5817 Bergen, Norway
    Vet Parasitol 118:169-74. 2003
  9. pmc beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels
    E M Glare
    Department of Respiratory Medicine, Alfred Hospital and Monash University Medical School, Melbourne 3181, Australia
    Thorax 57:765-70. 2002
  10. ncbi Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes
    Ashish Mogal
    Department of Pathology, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA
    Mol Cell Probes 20:81-6. 2006

Research Grants

Detail Information

Publications219 found, 100 shown here

  1. pmc Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
    Nicholas Silver
    Molecular Haematology, Division of Gene and Cell Based Therapy, King s College London School of Medicine at King s College Hospital, Denmark Hill, London, SE5 9PJ, UK
    BMC Mol Biol 7:33. 2006
    ..On this basis, stability of the candidate housekeeping genes was ranked according to repeatability of the gene expression differences among 31 samples...
  2. ncbi Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    T D Schmittgen
    Department of Pharmaceutical Sciences, College of Pharmacy, and the Cancer Prevention and Research Center, Washington State University, Pullman, WA 99164 6534, USA
    J Biochem Biophys Methods 46:69-81. 2000
    ..The internal control gene needs to be properly validated when designing quantitative gene expression studies...
  3. ncbi Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:54046-52. 2004
    ..These data suggest that GAPDH imparts a unique function necessary for membrane trafficking from VTCs that does not require GAPDH glycolytic activity...
  4. ncbi Differential expression of GAPDH and beta3-actin in growing collateral arteries
    Elisabeth Deindl
    Max Planck Institute, Department of Experimental Cardiology, Bad Nauheim, Germany
    Mol Cell Biochem 236:139-46. 2002
    ..From these results we conclude that the 18S rRNA, but not the GAPDH- or beta-actin mRNA is an appropriate internal control for relative quantitation of gene expression under conditions of cell proliferation in growing vessels...
  5. ncbi The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
    K Dheda
    Centre for Infectious Diseases and International Health, Royal Free and University College Medical School, and Department of Thoracic and HIV Medicine, Royal Free Hospital, London NW3 2QG, UK
    Anal Biochem 344:141-3. 2005
  6. ncbi Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications
    Simone Holtgrefe
    Department of Plant Physiology, University of Osnabrueck, D 49069 Osnabrueck, Germany
    Physiol Plant 133:211-28. 2008
    ....
  7. ncbi GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues
    Robert D Barber
    Pharmagene Laboratories Limited, Royston, Hertfordshire, United Kingdom
    Physiol Genomics 21:389-95. 2005
    ..These data establish comparative levels of expression and can be used to add value to gene expression data in which GAPDH is used as the internal control...
  8. ncbi Validation of reference genes for transcription profiling in the salmon louse, Lepeophtheirus salmonis, by quantitative real-time PCR
    Petter Frost
    Department of Aquaculture, Institute of Marine Research, P O Box 1870, Nordnes, N 5817 Bergen, Norway
    Vet Parasitol 118:169-74. 2003
    ..salmonis life cycle. Furthermore our results indicated that 18S RNA, although constitutively expressed, is not a convenient reference gene for relative quantification of most transcripts...
  9. pmc beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels
    E M Glare
    Department of Respiratory Medicine, Alfred Hospital and Monash University Medical School, Melbourne 3181, Australia
    Thorax 57:765-70. 2002
    ..We hypothesised that airway samples which differ in their cellular profiles and activation status have different levels of expression of GAPDH and beta-actin...
  10. ncbi Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes
    Ashish Mogal
    Department of Pathology, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA
    Mol Cell Probes 20:81-6. 2006
    ..However, RPL13A mRNA levels remained constant in all the conditions tested. Therefore, we recommend use of RPL13A as a standard for normalization during TSA treatment...
  11. ncbi Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase: a role in high glucose-induced apoptosis in retinal Müller cells
    Linda L Kusner
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, USA
    Invest Ophthalmol Vis Sci 45:1553-61. 2004
    ..In this study, the role of nuclear translocation of GAPDH in hyperglycemia-induced apoptosis in retinal Müller cells and the ability of R-(-)-deprenyl to inhibit the translocation of GAPDH and apoptosis were investigated...
  12. pmc Effect of RNA quality on transcript intensity levels in microarray analysis of human post-mortem brain tissues
    Tatiana Popova
    Boehringer Ingelheim Pharma GmbH Co and KG, Birkendorfer Str 65, Biberach and der Riss, Germany
    BMC Genomics 9:91. 2008
    ..On the other hand microarray data analysis associated with these studies is a challenging task. In this publication we address the issue of low RNA quality data and corresponding data analysis strategies...
  13. pmc Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility
    Kiyoshi Miki
    Laboratories for Reproductive Biology, and Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 101:16501-6. 2004
    ....
  14. ncbi New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells
    Michael A Sirover
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Cell Biochem 95:45-52. 2005
    ..Considerations of future directions as well as the role of GAPDH post-translational modification as a basis for its multifunctional activities is suggested...
  15. pmc The multifunctional protein glyceraldehyde-3-phosphate dehydrogenase is both regulated and controls colony-stimulating factor-1 messenger RNA stability in ovarian cancer
    Yi Zhou
    Arizona Cancer Center, University of Arizona, P O Box 245024, Tucson, AZ 85724 5024, USA
    Mol Cancer Res 6:1375-84. 2008
    ..In ovarian cancers, we show that GAPDH expression is regulated, and we now recognize that one of the many functions of GAPDH is to promote mRNA stability of CSF-1, an important cytokine in tumor progression...
  16. ncbi Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells
    Erik Willems
    Lab for Cell Genetics, Vrije Universiteit Brussel VUB, Brussels, Belgium
    Int J Dev Biol 50:627-35. 2006
    ..When looking for single stable reference genes, beta-actin works best in both mouse embryo and ES cell experiments and glyceraldehyde-3-phosphate-dehydrogenase can be applied in both mouse and human ES cell experiments...
  17. ncbi Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress
    Z Dastoor
    Institute of Biochemistry, University of Fribourg, CH-1700 Fribourg, Switzerland
    J Cell Sci 114:1643-53. 2001
    ..Our observations indicate that nuclear translocation of GAPDH may play a role in apoptosis and oxidative stress, probably related to the activity of GAPDH as a DNA repair enzyme or as a nuclear carrier for pro-apoptotic molecules...
  18. pmc A major surface protein on group A streptococci is a glyceraldehyde-3-phosphate-dehydrogenase with multiple binding activity
    V Pancholi
    Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021
    J Exp Med 176:415-26. 1992
    ..The multiple binding capacity of the SDH in conjunction with its GAPDH activity may play a role in the colonization, internalization, and the subsequent proliferation of group A streptococci...
  19. pmc Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture
    Michael T Forrester
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, USA
    Nat Biotechnol 27:557-9. 2009
    ..This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation...
  20. pmc Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae
    Daniel Shenton
    Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology UMIST, P O Box 88, Manchester M60 1QD, UK
    Biochem J 374:513-9. 2003
    ..In summary, protein S-thiolation appears to serve an adaptive function during exposure to an oxidative stress by reprogramming metabolism and protecting protein synthesis against irreversible oxidation...
  21. pmc Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis
    Nilkantha Sen
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Nat Cell Biol 10:866-73. 2008
    ..Our findings reveal a pathway in which NO-induced nuclear GAPDH mediates cell death through p300/CBP...
  22. ncbi Nuclear-encoded, plastid-targeted genes suggest a single common origin for apicomplexan and dinoflagellate plastids
    N M Fast
    Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada
    Mol Biol Evol 18:418-26. 2001
    ....
  23. ncbi Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    Carmela Tricarico
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Anal Biochem 309:293-300. 2002
    ..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies...
  24. ncbi The active site cysteine of the proapoptotic protein glyceraldehyde-3-phosphate dehydrogenase is essential in oxidative stress-induced aggregation and cell death
    Hidemitsu Nakajima
    Laboratory of Veterinary Pharmacology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen cho, Naka ku, Sakai City 599 8531, Japan
    J Biol Chem 282:26562-74. 2007
    ..Thus, oxidative stresses induce amyloid-like aggregation of GAPDH via aberrant disulfide bonds of the active site cysteine, and the formation of such abnormal aggregates promotes cell death...
  25. ncbi Irradiation of GAPDH: a model for environmentally induced protein damage
    Peter Voss
    Research Institute for Environmental Medicine, Molecular Aging Research, Heinrich Heine University Dusseldorf, D 40225 Dusseldorf, Germany
    Biol Chem 388:583-92. 2007
    ..The possibility of using the amino acid oxidation pattern as a biomarker of the source of damage is discussed...
  26. pmc A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, 540 East Canfield Avenue, 6374 Scott Hall, Detroit, MI 48201, USA
    Traffic 8:733-41. 2007
    ..Our results suggest that Src plays a pivotal role in regulating the interaction of Rab2 effectors on the VTC...
  27. ncbi Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel
    Latifa Fourrat
    Laboratoire de Physiologie et Genetique Moleculaire, Departement de Biologie, Faculte des Sciences Ain Chock, Universite Hassan II, Casablanca, Morocco
    Acta Biochim Biophys Sin (Shanghai) 39:148-54. 2007
    ..21+/-0.08 mM, respectively. The V(max) of the purified protein was estimated to be 52.7+/-5.9 U/mg. These kinetic parameter values were different from those described previously, reflecting protein differences between species...
  28. ncbi Both beta-actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer
    Ryutaro Mori
    Department of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Prostate 68:1555-60. 2008
    ..We investigated which housekeeping gene is useful to study gene expression of paraffin embedded human tissue samples of prostate cancer...
  29. ncbi Interaction of glyceraldehyde-3-phosphate dehydrogenase with secondary and tertiary RNA structural elements of the hepatitis A virus 3' translated and non-translated regions
    Günter Dollenmaier
    Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9001 St Gallen, Switzerland
    J Gen Virol 84:403-14. 2003
    ....
  30. ncbi Analysis of glucose metabolism in diabetic rat retinas
    M Shamsul Ola
    Dept of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, P O Box 850, Hershey, PA 17033, USA
    Am J Physiol Endocrinol Metab 290:E1057-67. 2006
    ..Metabolites upstream of glucose such as the sorbitol pathway (which decreases NADPH) and polyol synthesis are increased...
  31. ncbi Glyceraldehyde-3-phosphate dehydrogenase binds to the AU-Rich 3' untranslated region of colony-stimulating factor-1 (CSF-1) messenger RNA in human ovarian cancer cells: possible role in CSF-1 posttranscriptional regulation and tumor phenotype
    Nathalie Bonafe
    Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Yale University School of Medicine, New Haven, Connecticut, USA
    Cancer Res 65:3762-71. 2005
    ....
  32. ncbi The GapA/B gene duplication marks the origin of Streptophyta (charophytes and land plants)
    Jörn Petersen
    Institut fur Genetik, Technische Universitat Braunschweig, Braunschweig, Germany
    Mol Biol Evol 23:1109-18. 2006
    ..This innovation may be one prerequisite for the development of storage tissues in land plants...
  33. pmc Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues
    M Piechaczyk
    Nucleic Acids Res 12:6951-63. 1984
    ..We therefore conclude that the tissue-specific differential GAPDH gene expression is regulated by adjusting the abundance of its mRNA at the post-transcriptional level...
  34. ncbi An enigmatic GAPDH gene in the symbiotic dinoflagellate genus Symbiodinium and its related species (the order Suessiales): possible lateral gene transfer between two eukaryotic algae, dinoflagellate and euglenophyte
    Kiyotaka Takishita
    Marine Biotechnology Institute, Kamaishi, Iwate 026 0001, Japan
    Protist 154:443-54. 2003
    ..It appears highly likely that at least two genes (cytosolic and plastid-targeted GAPDH genes) have been laterally transferred between these two eukaryotic algal groups...
  35. ncbi Validation of internal control genes for gene expression analysis in diabetic glomerulosclerosis
    Jason Biederman
    Division of Nephrology and Hypertension, Henry Ford Hospital, Detroit, Michigan 48202, USA
    Kidney Int 66:2308-14. 2004
    ....
  36. ncbi Reference genes identified in SH-SY5Y cells using custom-made gene arrays with validation by quantitative polymerase chain reaction
    Frederic J Hoerndli
    Division of Psychiatry Research, University of Zurich, August Forel Strasse 1, 8008 Zurich, Switzerland
    Anal Biochem 335:30-41. 2004
    ..By doing so, we were able to identify GAPD, M-RIP, and POLR2F as stable and usable reference genes irrespective of differentiation status and Abeta(42) treatment...
  37. pmc Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells
    Nitin Nitin
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Nucleic Acids Res 32:e58. 2004
    ..The peptide-linked molecular beacons approach promises to open new and exciting opportunities in sensitive gene detection and quantification in vivo...
  38. ncbi Housekeeping gene expression during fetal brain development in the rat-validation by semi-quantitative RT-PCR
    Maie Dawoud Al-Bader
    Department of Physiology, Faculty of Medicine, Kuwait University, P O Box 24923, Safat, Zip Code 13110, Kuwait
    Brain Res Dev Brain Res 156:38-45. 2005
    ..These results suggest that the choice of a housekeeping gene is critical to the interpretation of experimental results and should be modified according to the nature of the study...
  39. ncbi A novel CRM1-mediated nuclear export signal governs nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase following genotoxic stress
    Victor M Brown
    Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Biol Chem 279:5984-92. 2004
    ..Similar sequences (KKVV*7-13PLK) were found in two other human proteins, U5 small nuclear ribonucleoprotein, and transcription factor BT3...
  40. ncbi Housekeeping genes revisited: different expressions depending on gender, brain area and stressor
    N M Derks
    Department of Cellular Animal Physiology, Radboud University Nijmegen, P O Box 9010, 6500 GL Nijmegen, The Netherlands
    Neuroscience 156:305-9. 2008
    ....
  41. pmc Evidence for a chimeric nature of nuclear genomes: eubacterial origin of eukaryotic glyceraldehyde-3-phosphate dehydrogenase genes
    W Martin
    Institut fur Genetik, Technische Universitat Braunschweig, Germany
    Proc Natl Acad Sci U S A 90:8692-6. 1993
    ..Evidence that the eukaryotic nuclear genes for glycolytic GAPDH, as well as the Calvin-cycle genes, are of eubacterial origin suggests that eukaryotic genomes are more highly chimeric than previously assumed...
  42. ncbi Comparison of reverse transcriptases in gene expression analysis
    Anders Ståhlberg
    Department of Chemistry and Biosciences, Chalmers University of Technology, Gothenburg, Sweden
    Clin Chem 50:1678-80. 2004
  43. pmc De-regulation of common housekeeping genes in hepatocellular carcinoma
    Samuel Waxman
    Mount Sinai School of Medicine, Department of Medicine, Division of Hematology Oncology, New York, NY, USA
    BMC Genomics 8:243. 2007
    ..This study assessed eight stages of hepatitis C virus (HCV) induced hepatocellular carcinoma (HCC) to search for appropriate genes for normalization...
  44. ncbi Phylogeny of nuclear-encoded plastid-targeted GAPDH gene supports separate origins for the peridinin- and the fucoxanthin derivative-containing plastids of dinoflagellates
    Kiyotaka Takishita
    Japan Agency for Marine Earth Science and Technology, Yokosuka, Kanagawa 237 0061, Japan
    Protist 155:447-58. 2004
    ..The present results strongly support the hypothesis that the plastids of the peridinin- and the fucoxanthin derivative-containing dinoflagellates are of separate origin...
  45. ncbi HIF-2alpha regulates glyceraldehyde-3-phosphate dehydrogenase expression in endothelial cells
    Krista K Graven
    Department of Medicine, Section of Pulmonary and Critical Care, University of Wisconsin Medical School, 2590 Medical Sciences Center, 1300 University Avenue, Madison, WI 53706, USA
    Biochim Biophys Acta 1626:10-8. 2003
    ..These findings suggest that cell-specific patterns of HIF-1alpha and HIF-2alpha expression lead to cell-specific gene upregulation during hypoxia...
  46. ncbi Evidence for the two phosphate binding sites of an analogue of the thioacyl intermediate for the Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase-catalyzed reaction, from its crystal structure
    Marcelo S Castilho
    Instituto de Fisica de Sao Carlos, USP, P O Box 369, 13560 970 Sao Carlos, SP, Brazil
    Biochemistry 42:7143-51. 2003
    ..This result gives strong experimental support for the generally accepted flip-flop model of the catalytic mechanism in GAPDH...
  47. ncbi Vascular endothelial growth factor gene expression in middle cerebral artery occlusion in the rat
    F Lennmyr
    Department of Surgical Sciences, Section of Anesthesiology and Intensive Care, University Hospital, Uppsala University, Uppsala, Sweden
    Acta Anaesthesiol Scand 49:488-93. 2005
    ..In the present report we have investigated the concomitant changes in VEGF and glyceraldehyde dehydrogenase (GAPDH) mRNA expression in a model of permanent and transient cerebral ischemia...
  48. ncbi GAPDH as a sensor of NO stress
    Makoto R Hara
    Department of Neuroscience, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Biochim Biophys Acta 1762:502-9. 2006
    ..The NO/GAPDH/Siah1 death cascade will contribute to the molecular understanding of a role of GAPDH in neurodegenerative disorders and help to establish novel therapeutic strategies...
  49. ncbi Validation of two reference genes for mRNA level studies of murine disease models in neurobiology
    Michael Meldgaard
    Medical Biotechnology Center, University of Southern Denmark, Winsløwparken 25, DK 5000 Odense, Denmark
    J Neurosci Methods 156:101-10. 2006
    ..We furthermore demonstrate that above a certain threshold of test mRNA level changes and given high quality RNA processing, normalization to total RNA alone provides for equally reliable quantitative mRNA level results...
  50. ncbi Crystallization of three key glycolytic enzymes of the opportunistic pathogen Cryptosporidium parvum
    Olga Senkovich
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochim Biophys Acta 1750:166-72. 2005
    ..X-ray diffraction data have been collected for all crystals...
  51. pmc Highly efficient production of laccase by the basidiomycete Pycnoporus cinnabarinus
    Alexandra M C R Alves
    Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Haren, The Netherlands
    Appl Environ Microbiol 70:6379-84. 2004
    ..2 g of laccase per liter and thus representing the highest laccase activities reported for recombinant fungal strains. These results suggest that P. cinnabarinus may be a host of choice for the production of other proteins as well...
  52. ncbi Nuclear localization of glyceraldehyde-3-phosphate dehydrogenase is not involved in the initiation of apoptosis induced by 1-Methyl-4-phenyl-pyridium iodide (MPP+)
    Rieko Kodama
    Department of Neurology, Institute of Advanced Medicine, Wakayama Medical University, Japan
    Genes Cells 10:1211-9. 2005
    ..Thus, we have concluded that a simple nuclear localization of GAPDH does not induce apoptosis, and that MPP+-induced apoptosis is not caused by nuclear translocation of GAPDH...
  53. ncbi Glyceraldehyde-3-phosphate dehydrogenase is a GABAA receptor kinase linking glycolysis to neuronal inhibition
    Jacques J Laschet
    Laboratory of Molecular Neurobiology and Pharmacology, Institut National de la Santé et de la Recherche Médicale Unité 573, F 75014 Paris, France
    J Neurosci 24:7614-22. 2004
    ..Our results provide a molecular mechanism for the direct involvement of glycolysis in neurotransmission...
  54. ncbi Limitations of commonly used internal controls for real-time RT-PCR analysis of renal epithelial-mesenchymal cell transition
    Gerard Elberg
    Department of Pediatrics, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nephron Exp Nephrol 102:e113-22. 2006
    ..We report herein the limitations of commonly used reference genes for mRNA quantitation...
  55. ncbi Nucleus-encoded, plastid-targeted glyceraldehyde-3-phosphate dehydrogenase (GAPDH) indicates a single origin for chromalveolate plastids
    James T Harper
    Canadian Institute for Advanced Research, Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada
    Mol Biol Evol 20:1730-5. 2003
    ....
  56. ncbi Crystal structure of two ternary complexes of phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus with NAD and D-glyceraldehyde 3-phosphate
    Claude Didierjean
    Laboratoire de Cristallographie et de Modélisation des Matériaux Minéraux et Biologiques, Groupe Biocristallographie, UMR 7036, CNRS Université Henri Poincaré, Faculte des Sciences, 54506 Vandoeuvre Cedex, France
    J Biol Chem 278:12968-76. 2003
    ..Arguments are provided that the structures reported here are representative of a productive enzyme.NAD.D-G3P complex in the ground state (Michaelis complex)...
  57. ncbi Maternal diabetes in vivo and high glucose in vitro diminish GAPDH activity in rat embryos
    Parri Wentzel
    Department of Medical Cell Biology, Biomedical Center, Uppsala University, PO Box 571, SE 751 23 Uppsala, Sweden
    Diabetes 52:1222-8. 2003
    ..In conclusion, dysmorphogenesis caused by maternal diabetes is correlated with ROS-induced inhibition of GAPDH in embryos, which could indicate that inhibition of GAPDH plays a causal role in diabetic embryopathy...
  58. ncbi Hypoxia up-regulates glyceraldehyde-3-phosphate dehydrogenase in mouse brain capillary endothelial cells: involvement of Na+/Ca2+ exchanger
    Ryoichi Yamaji
    Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, 5998531, Osaka, Japan
    Biochim Biophys Acta 1593:269-76. 2003
    ..However, the GAPDH activity did not rise in proportion to the increase of GAPDH protein by hypoxia even in the cytosolic fraction. These results suggest that not all hypoxia-induced GAPDH molecules contribute to glycolysis...
  59. ncbi The use of housekeeping genes for real-time PCR-based quantification of fusion gene transcripts in acute myeloid leukemia
    M Weisser
    Leukemia 18:1551-3. 2004
  60. ncbi Structure of Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase complexed with chalepin, a natural product inhibitor, at 1.95 A resolution
    F Pavão
    Instituto de Quimica de Sao Carlos, USP, Sao Carlos, SP, Brazil
    FEBS Lett 520:13-7. 2002
    ..The final refined model of the complex shows extensive conformational changes when compared with the native structure. The mode of binding of chalepin to gGAPDH and its implications for inhibitor design are discussed...
  61. ncbi GAPDH gene diversity in spirochetes: a paradigm for genetic promiscuity
    R M Figge
    Institut fur Genetik, Technische Universitat Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany
    Mol Biol Evol 18:2240-9. 2001
    ..Furthermore, we provide evidence that the GAPDH genes present in the protists Parabasalia may also be of spirochetal descent...
  62. ncbi Expression of mRNA encoding G protein-coupled receptors involved in congestive heart failure--a quantitative RT-PCR study and the question of normalisation
    Trond Brattelid
    Dept of Pharmacology, University of Oslo, 1057, Blindern, 0316 Oslo, Norway
    Basic Res Cardiol 102:198-208. 2007
    ..Q-RT-PCR is a sensitive and powerful method to monitor changes in GPCR mRNA expression in CHF. However, the normalisation standard used is important for the interpretation of mRNA regulation...
  63. ncbi Minireview. Emerging new functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells
    M A Sirover
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Life Sci 58:2271-7. 1996
    ..These findings have the potential to alter our basic understanding of the molecular mechanisms through which human or mammalian cells utilize individual proteins in vital, yet unrelated, cell processes...
  64. ncbi Glyceraldehyde-3-phosphate dehydrogenase exerts different biologic activities in apoptotic and proliferating hepatocytes according to its subcellular localization
    Luciana Barbini
    Departamento de Fisiologia, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus Universitario, 27002 Lugo, Spain
    Mol Cell Biochem 300:19-28. 2007
    ..In conclusion, GAPDH plays different roles or biological activities in proliferating and apoptotic hepatocytes, according to its subcellular localization...
  65. ncbi Glyceraldehyde-3-phosphate dehydrogenase and apoptosis
    M D Berry
    Alviva Biopharmaceuticals, Inc, Suite 112C, 116, Research Drive, Innovation Place, Saskatoon, Saskatchewan, Canada
    J Neurosci Res 60:150-4. 2000
    ..This review summarizes the evidence implicating GAPDH in the initiation of one or more apoptotic cascades. The possible functions of GAPDH in the nucleus which could result in the initiation of apoptosis are also discussed...
  66. ncbi Compartment-specific isoforms of TPI and GAPDH are imported into diatom mitochondria as a fusion protein: evidence in favor of a mitochondrial origin of the eukaryotic glycolytic pathway
    M F Liaud
    Institute of Genetics, University of Braunschweig, Germany
    Mol Biol Evol 17:213-23. 2000
    ....
  67. ncbi Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene
    K K Graven
    The Pulmonary Center, Boston University School of Medicine, 715 Albany Street, R 304, Boston, MA 02118, USA
    Biochim Biophys Acta 1447:208-18. 1999
    ..Furthermore, the novel HIF-1 flanking sequence required for GAPDH upregulation and the protein(s) that bind to it may be EC specific...
  68. ncbi Glyceraldehyde-3-phosphate dehydrogenase expression during apoptosis and proliferation of rat ventral prostate
    D E Epner
    Department of Medicine, Baylor College of Medicine and VA Medical Center, Houston, Texas 77030, USA
    Biol Reprod 61:687-91. 1999
    ..Our studies suggest that GAPDH plays multiple roles during prostate epithelial cell apoptosis and proliferation...
  69. ncbi New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase
    M A Sirover
    Department of Pharmacology, Temple University School of Medicine, Philadelphia PA 19140, USA
    Biochim Biophys Acta 1432:159-84. 1999
    ..The interrelationship between new GAPDH activities and its role in cell pathologies is addressed...
  70. pmc GAPDH is not regulated in human glioblastoma under hypoxic conditions
    Harun M Said
    University of Wurzburg, Dept of Radiation Oncology, Germany
    BMC Mol Biol 8:55. 2007
    ..HIF-1alpha protein regulation under hypoxia was also determined on mRNA level in vitro in GaMG and on protein level in U251, U373 and GaMG cells...
  71. ncbi Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
    H Zhong
    Johns Hopkins Oncology Center, Brady Urological Institute, Baltimore, Maryland, 21287 2411, USA
    Biochem Biophys Res Commun 259:523-6. 1999
    ....
  72. ncbi Evaluation and validation of housekeeping genes in response to ionizing radiation and chemical exposure for normalizing RNA expression in real-time PCR
    Malathi Banda
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Mutat Res 649:126-34. 2008
    ....
  73. ncbi Origins of plastids and glyceraldehyde-3-phosphate dehydrogenase genes in the green-colored dinoflagellate Lepidodinium chlorophorum
    Kiyotaka Takishita
    Japan Agency for Marine Earth Science and Technology JAMSTEC, Yokosuka, Kanagawa, Japan
    Gene 410:26-36. 2008
    ..However, in the light of Lepidodinium GAPDH data, we need to closely examine whether the monophyly of the plastids in the above lineages inferred from plastid-targeted GapC genes truly reflects that of the host lineages...
  74. ncbi The rational design of trypanocidal drugs: selective inhibition of the glyceraldehyde-3-phosphate dehydrogenase in Trypanosomatidae
    M Callens
    Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Brussels, Belgium
    Ann Trop Med Parasitol 89:23-30. 1995
    ..2 and 0.3 mM, respectively. Moreover, the inhibition of the parasite enzyme is 45-fold (T. brucei) or 170-fold (L. mexicana) greater with this substituted analogue than that produced with adenosine...
  75. ncbi Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes
    L McAlister
    J Biol Chem 260:15019-27. 1985
    ..These data confirm that the three yeast glyceraldehyde-3-phosphate dehydrogenase genes encode catalytically active enzyme and that the genes are expressed at different levels during logarithmic cell growth...
  76. ncbi Structure of holo-glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus at 1.8 A resolution
    T Skarzynski
    J Mol Biol 193:171-87. 1987
    ..A significant number of buried water molecules have been found that play an important role in the structural integrity of the molecule...
  77. ncbi Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene
    L Ercolani
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114
    J Biol Chem 263:15335-41. 1988
    ..In contrast, a probe derived from an intron segment of the isolated gene detected only a single copy of the GAPDH gene. Collectively, these findings strongly suggest that the human genome encodes a single functional GAPDH gene...
  78. ncbi Structural analysis of glyceraldehyde 3-phosphate dehydrogenase from Escherichia coli: direct evidence of substrate binding and cofactor-induced conformational changes
    M Yun
    Department of Structural Biology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38105, USA
    Biochemistry 39:10702-10. 2000
    ..This finding suggests that the cofactor NAD(+) may stabilize the transition state oxyanion of the hemiacetal intermediate in support of the flip-flop model for GAP binding...
  79. ncbi Proliferative and nutritional dependent regulation of glyceraldehyde-3-phosphate dehydrogenase expression in the rat liver
    I R Corbin
    Liver Diseases Unit, Department of Medicine and Pharmacology, University of Manitoba, Winnipeg, Canada
    Cell Prolif 35:173-82. 2002
    ..The results also indicate that glyceraldehyde-3-phosphate dehydrogenase expression is affected by the nutritional status of animals undergoing abdominal sham surgery...
  80. ncbi Identification of an additional hypoxia responsive element in the glyceraldehyde-3-phosphate dehydrogenase gene promoter
    Shan Lu
    Department of Medicine, Baylor College of Medicine, VA Medical Center, Houston, TX 77030, USA
    Biochim Biophys Acta 1574:152-6. 2002
    ..Mutation of HIF-1 sites within the novel HRE resulted in complete loss of function...
  81. ncbi Glyceraldehyde-3-phospate dehydrogenase is translocated into nuclei through Golgi apparatus during apoptosis induced by 6-hydroxydopamine in human dopaminergic SH-SY5Y cells
    Wakako Maruyama
    Laboratory of Biochemistry and Metabolism, Department of Basic Gerontology, National Institute for Longevity Sciences, Obu, Aichi 474 8522, Japan
    Neurosci Lett 321:29-32. 2002
    ..These results suggest that the Golgi apparatus may be involved in the compartmentalization of GAPDH from cytosol to nuclei...
  82. ncbi Concerted evolution in the GAPDH family of retrotransposed pseudogenes
    P Garcia-Meunier
    Laboratoire genome et populations, CNRS URA 1493, Universite de Montpellier II, France
    Mamm Genome 4:695-703. 1993
    ..The homogenization we observe may be due to the pseudogenes themselves (concerted evolution in a strict sense), which explains the occurrence of long-term homogenization of old sequences and subfamily groupings...
  83. ncbi Reversible nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase upon serum depletion
    H D Schmitz
    Biocentre, Goethe University of Frankfurt, Germany
    Eur J Cell Biol 80:419-27. 2001
    ..Thus, the nuclear accumulation of GAPDH upon growth factor depletion is a reversible process not related directly to cell cycle and likely triggered by survival signals...
  84. ncbi Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes
    K Gorzelniak
    Franz-Volhard-Klinik, , , Germany
    Horm Metab Res 33:625-7. 2001
    ..Only GAPDH and transferrin receptor gene expression levels did not change under any of the stimuli tested, thus appeared best suited for gene expression studies in human adipose cells across a wide range of experimental settings...
  85. ncbi Glyceraldehyde-3-phosphate dehydrogenase is negatively regulated by ADP-ribosylation in the fungus Phycomyces blakesleeanus
    M Deveze-Alvarez
    , , Universidad de Guanajuato, Apdo. postal 187, Guanajuato, Gto, 36000, Mexico
    Microbiology 147:2579-84. 2001
    ..ADP-ribosylation of the GAPDH occurred by transfer of the ADP-ribose moiety from NAD to an arginine residue. A model for the regulation of GAPDH activity and its role in spore germination in P. blakesleeanus is proposed...
  86. pmc Adenosine analogues as selective inhibitors of glyceraldehyde-3-phosphate dehydrogenase of Trypanosomatidae via structure-based drug design
    J C Bressi
    Department of Chemistry, University of Washington, Seattle, WA 98195, USA
    J Med Chem 44:2080-93. 2001
    ..We also explored adenosine analogues containing 5'-amido substituents and found that 2',5'-dideoxy-2'-(3,5-dimethoxybenzamido)-5'-(diphenylacetamido)adenosine (49) displays an IC(50) of 60-100 microM against the three parasite GAPDH's...
  87. ncbi Validity of messenger RNA expression analyses of human saliva
    Shalini V Kumar
    Laboratory of Human Toxicology and Molecular Epidemiology, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    Clin Cancer Res 12:5033-9. 2006
    ..The origins of expression microarray and reverse transcription-PCR (RT-PCR) signals in human saliva were evaluated...
  88. ncbi Effects of dimethyl sulfoxide and dexamethasone on mRNA expression of housekeeping genes in cultures of C2C12 myotubes
    Masuhiro Nishimura
    New Product and Business Development, Research and Development Center, Otsuka Pharmaceutical Factory, Inc, 115 Tateiwa, Muya cho, Naruto, Tokushima 772 8601, Japan
    Biochem Biophys Res Commun 367:603-8. 2008
    ..The GUSB, PPIA, and TFRC mRNA levels were unaffected by exposure to Dex. GUSB, HPRT1, and PPIA are thus suitable internal controls for evaluating mRNA expression levels in cultures of C2C12 cells...
  89. ncbi Genetic polymorphism of aldehyde oxidase in Donryu rats
    Kunio Itoh
    Department of Drug Metabolism and Pharmacokinetics, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Aoba ku, Sendai 981 8558, Japan
    Drug Metab Dispos 35:734-9. 2007
    ..If a new drug candidate is primarily metabolized by aldehyde oxidase, attention should be given to using a rat strain with high aldehyde oxidase activity and small individual variation...
  90. ncbi Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II
    David Baillat
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Cell 123:265-76. 2005
    ..The Integrator complex is evolutionarily conserved in metazoans and directly interacts with the C-terminal domain of the RNA polymerase II largest subunit...
  91. ncbi Quantitative analysis of messenger RNA abundance for ribosomal protein L-15, cyclophilin-A, phosphoglycerokinase, beta-glucuronidase, glyceraldehyde 3-phosphate dehydrogenase, beta-actin, and histone H2A during bovine oocyte maturation and early embryogen
    Anilkumar Bettegowda
    Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, Michigan 48824, USA
    Mol Reprod Dev 73:267-78. 2006
    ..Results demonstrate a valid approach for quantitative analysis of mRNA abundance in oocytes and embryos, but do not support constitutive expression of above genes during early embryonic development...
  92. ncbi Renal vascular endothelial growth factor in neonatal obstructive nephropathy. I. Endogenous VEGF
    Laura E Burt
    Dept of Pediatrics, Univ of Virginia, Box 800386, Charlottesville, VA 22908, USA
    Am J Physiol Renal Physiol 292:F158-67. 2007
    ..Following UUO in the adult, the VEGF receptor is upregulated. Endogenous VEGF may serve an adaptive role in responding to tubular injury caused by UUO and may modulate adaptation by the contralateral kidney...
  93. ncbi Post-mortem changes in gene expression of the muscle tissue of rainbow trout, Oncorhynchus mykiss
    Masataka Saito
    Laboratory of Food Science and Technology, Kagawa Nutrition University, Chiyoda, Sakado, Saitama, Japan
    J Agric Food Chem 54:9417-21. 2006
    ..These results suggest that the increased mRNAs in rainbow trout muscle occurred by transcription immediately after death...
  94. ncbi The evaluation of ghrelin mRNA expression in human somatotroph adenomas
    Ryszard Wasko
    Department of Endocrinology, Metabolism and Internal Diseases, Karol Marcinkowski University of Medical Sciences in Poznan, Poland
    Neuro Endocrinol Lett 27:169-73. 2006
    ..We postulate that the absence of the ghrelin gene transcript is mainly due to the treatment with somatostatin analogues administered preoperatively, which could have suppressed the ghrelin gene transcription...
  95. ncbi Cloning and pharmacological characterization of the dog histamine H4 receptor
    Wen Jiang
    Johnson and Johnson Pharmaceutical Research and Development, L L C, 3210 Merryfield Row, San Diego, CA 92121, United States
    Eur J Pharmacol 592:26-32. 2008
    ..The cloning and in vitro pharmacological characterization of the dog histamine H4 receptor provide useful information for future studies using dog models as well as in understanding the ligand-receptor interactions of the receptor...
  96. ncbi GABA(A) alpha1 and alpha2 receptor subunit expression in rostral ventrolateral medulla in nonpregnant and pregnant rats
    C Michael Foley
    Department of Biomedical Sciences, Dalton Cardiovascular Research Center, 134 Research Park Drive, University of Missouri Columbia, Columbia, MO 65211 3300, USA
    Brain Res 975:196-206. 2003
    ....
  97. ncbi Essential role of Rac1/NADPH oxidase in nerve growth factor induction of TRPV1 expression
    Preeti Puntambekar
    Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9629, USA
    J Neurochem 95:1689-703. 2005
    ..We conclude that the Rac1/NADPH oxidase pathway regulates p38 activation and TRPV1 expression which aids in the maintenance of peripheral neuron integrity and pain perception...
  98. ncbi Recovery of exfoliated cells from the gastrointestinal tract of premature infants: a new tool to perform "noninvasive biopsies?"
    Bertrand Kaeffer
    UMR 1280, Physiologie des Adaptations Nutritionnelles, F 44093 Nantes cedex 1, France
    Pediatr Res 62:564-9. 2007
    ..Exfoliated cells can be recovered from gastric aspirates and fecal samples and serve as a tool to investigate the impact of therapeutic and nutritional regimens on the maturation of GI functions...
  99. ncbi Evaluation of various housekeeping genes for their applicability for normalization of mRNA expression in dioxin-treated rats
    Raimo Pohjanvirta
    Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
    Chem Biol Interact 160:134-49. 2006
    ..These findings show that normalization genes for qRT-PCR must be carefully validated in advance, especially if the study involves a potent modifier of gene expression...
  100. ncbi Bifunctional modulating effects of an indigo dimer (bisindigotin) to CYP1A1 induction in H4IIE cells
    K P Lai
    Croucher Institute of Environmental Sciences and Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China
    Toxicology 226:188-96. 2006
    ..Nevertheless our results indicate that SLY-1 is not only an inducer of the CYP1A1 system, but also a potent inhibitor of CYP1A1 enzyme...
  101. ncbi Expression of alphasmooth muscle actin in lens epithelia from human donors and cataract patients
    Elisabeth Rungger-Brandle
    Cell Biology Laboratory, University Eye Clinic, 22, Rue Alcide Jentzer, CH 1211 Geneve, Switzerland
    Exp Eye Res 81:539-50. 2005
    ..In pathologic situations such as PSO and ASF, persisting stimulation and additional incentives may induce increased alphaSMA expression and more elaborate patterning, eventually leading to completion of EMT...

Research Grants34

  1. VTC Events Mediated by the Rab2 Complex
    Ellen J Tisdale; Fiscal Year: 2010
    ..The studies proposed in this application will develop a new and novel paradigm that couples Rab2-Apkc lA-GAPDH dysfunction with pathological consequences. ..
  2. MECHANISMS OF ENDOTHELIAL GAPDH REGULATION BY HYPOXIA
    KRISTA GRAVEN; Fiscal Year: 2002
    ..abstract_text> ..
  3. Cytokines on Olfactory Receptor Neuron Function
    Andrew Lane; Fiscal Year: 2006
    ..Additional bench research will be performed in the laboratory of Dr. Schleimer, an established scientist with expertise in airway inflammation and cytokine modulation of cell function. ..
  4. PROTEOMIC ANALYSIS OF DECREASED AUTOPHAGY IN AGING
    Ana Cuervo; Fiscal Year: 2005
    ..abstract_text> ..
  5. Mentored Patient-Oriented Research Career Development Award
    Ruey Kang Chang; Fiscal Year: 2006
    ..The principal investigator will take advanced courses to enhance knowledge and skills needed for patient-oriented research and work with the mentors to conduct the proposed project during the award period. ..
  6. Gordon Conference-Autophagy/Stress/Development/Disease
    Ana Cuervo; Fiscal Year: 2007
    ....
  7. Steroid influences on brain maturation
    JULIA ZEHR; Fiscal Year: 2006
    ..A basic understanding of the processes controlling pubertal brain development provides a timely foundation for future studies of self-administered steroids, disease states, or environmental endocrine disrupters. ..
  8. New Mechanism of Carcinogen and Antioxidant Detection
    MICHAEL SIROVER; Fiscal Year: 2006
    ..En toto, through this exploratory study, we shall define a new mechanism through which the generation of endothelial cell ROS may be used as a detection mechanism for potential carcinogens or therapeutic antioxidants. ..
  9. Alveolar Biology: Sex, Age, and Alveolar Turnover
    Donald Massaro; Fiscal Year: 2007
    ..abstract_text> ..
  10. Molecular Analysis of the Human Aqueous Outflow Pathway
    Michael P Fautsch; Fiscal Year: 2010
    ..We will also determine gene and protein expression changes in the meshwork induced by normal and POAG aqueous humor. Develop a medium that can be used to mimic aqueous humor for trabecular meshwork cell culture. ..
  11. Cortical Dynamics for Pain Perception in Behaving Rats
    APKAR APKARIAN; Fiscal Year: 2005
    ..abstract_text> ..
  12. Photoreceptor Cell Vulnerability and Glutathione Status
    Barry S Winkler; Fiscal Year: 2010
    ..These outcomes should help in the design of therapeutic treatments for photoreceptor degenerations that are linked to an inadequate antioxidative defense mechanism. ..
  13. Gap Junction and Bone Cell Responses to Physical Signals
    Henry J Donahue; Fiscal Year: 2010
    ..Additionally, an understanding of how mechanical signals regulate bone cell proliferation and differentiation would be beneficial in designing in vitro environments, i.e. bioreactors, for novel bone tissue engineering protocols. ..
  14. PERMISSIVE RETINAL GENE EXPRESSION VIA HYPOMETHYLATION
    JEFFREY BOATRIGHT; Fiscal Year: 2001
    ..abstract_text> ..
  15. EXPERIMENTAL EMPHYSEMA--STUDIES ON REMEDIATION
    Donald Massaro; Fiscal Year: 2001
    ....
  16. REMEDIATION OF EMPHYSEMA--MECHANISMS, CHARACTERISTICS
    Donald Massaro; Fiscal Year: 2001
    ....
  17. GAPDH--NOVEL REGULATOR OF CELL STRUCTURE AND MIGRATION
    Daniel Epner; Fiscal Year: 2002
    ..Large portions of GAPDH will then be deleted from the fusion protein to determine which regions other than the actin-binding domain, if any, are critical for actin localization. ..
  18. Effect of diflunisal (IND68092) on familial amyloidosis
    JOHN BERK; Fiscal Year: 2009
    ..poiuytrewq c Diflunisal drug levels will be determined to assess treatment compliance. We will correlate FAP disease progression with TTR stabilization. ..
  19. GLUTAMINE AND BREAST CANCER PROLIFERATION AND TREATMENT
    STEVE ABCOUWER; Fiscal Year: 2002
    ..This may provide the basis for novel metabolically directed cancer therapies. ..
  20. POST-TRANSLATIONAL MECHANISMS IN URACIL-DNA REPAIR
    SALVATORE CARADONNA; Fiscal Year: 2004
    ..Our data indicate another level of regulation of this DNA repair enzyme. Since UDG1A is the first step in this DNA repair pathway, regulation of UDG1A activity will dictate control over the enitre pathway. ..
  21. Transcriptional Regulation of PDGF Receptor Alpha
    Jane Reusch; Fiscal Year: 2009
    ..Determine how PDGFRa expression is affected by changing concentrations and activation states of CREB and C/EBPdelta. 3. To determine whether PPARa mediated suppression of PDGFRa gene expression works through CREB and C/EBPs. ..
  22. CELLULAR BIOLOGY OF THE TRABECULAR MESHWORK
    Michael P Fautsch; Fiscal Year: 2010
    ..These regions will be "preferential flow" regions, and aqueous tracers should appear in these regions first. If this occurs, it would change our basic understanding of how aqueous passes through the TM (aim 5). ..
  23. Experimental COPD: remediation, mitigation
    DONALD JOHN MASSARO; Fiscal Year: 2010
    ..There is not treatment that slows the relentless progression of the disease or that reverses it. In this application, we use an animal model to investigate methods to slow progression and reverse the disease. ..
  24. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  25. IC Communication in Breast Cancer Metastasis to Bone
    Henry Donahue; Fiscal Year: 2005
    ..The results of this project will provide insights into the mechanism by which breast cancer cells preferentially metastasize to bone, an important step in developing approaches to inhibit bone metastasis. ..
  26. ARSENIC TRIOXIDE TREATMENT OF LYMPHOPROLIFERATIVE DISORD
    Samuel Waxman; Fiscal Year: 2003
    ..Should our laboratory study identify agents or schedules that enhance the response to As203, we will use them to appropriately modify the initial phase II pilot study. ..
  27. Characterizing temporal dynamics of spontaneous pain
    APKAR APKARIAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  28. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  29. Molecular Detection of Occult Neuroblastoma
    Irene Cheung; Fiscal Year: 2004
    ..If these two molecular markers of MRD can independently predict patient outcome, they will in the future provide an objective endpoint for stopping further treatment, and serve as sensitive surrogates for evaluating adjuvant therapies. ..
  30. METABOLIC AND HOMEOSTATIC MECHANISMS IN RETINA AND RPE
    Barry Winkler; Fiscal Year: 2003
    ..g., diamox) which have been reported to decrease retinal edema. A long-term goal of the second project is to understand better the nature of the interactions between the retina and RPE and factors regulating retinal detachment. ..
  31. Trichomonas vaginalis: Molecular Analysis of Adherence
    JOHN ALDERETE; Fiscal Year: 2007
    ..abstract_text> ..
  32. Role of estrogen receptor beta in cancer prevention by dietary indole-3-carbinol
    Abby Benninghoff; Fiscal Year: 2008
    ..abstract_text> ..
  33. Survival and Age Biases in Gene Associations with Coronary Disease
    Jennifer R Dungan; Fiscal Year: 2010
    ..This research may help improve health outcomes and survival for people with heart disease. ..