nitrite reductases

Summary

Summary: A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1.

Top Publications

  1. ncbi Bacterial nitric oxide synthesis
    F Cutruzzola
    Dipartimento di Scienze Biochimiche, Universita di Roma La Sapienza, p le A Moro, 5, 00185, Rome, Italy
    Biochim Biophys Acta 1411:231-49. 1999
  2. ncbi Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus)
    Wladyslaw Polcyn
    Department of Plant Physiology, Faculty of Biology, A Mickiewicz University, Al Niepodleglosci 14, 61 713 Poznan, Poland
    FEMS Microbiol Lett 226:331-7. 2003
  3. pmc Reciprocal regulation of cellular nitric oxide formation by nitric oxide synthase and nitrite reductases
    George B Stefano
    Neuroscience Research Institute, State University of New York College at Old Westbury, Old Westbury, NY 11568 0210, USA
    Med Sci Monit 17:RA221-6. 2011
  4. ncbi Homologues of nitrite reductases in ammonia-oxidizing archaea: diversity and genomic context
    Rita Bartossek
    Department of Biology, Centre for Geobiology, University of Bergen, PO Box 7803, 5020 Bergen, Norway
    Environ Microbiol 12:1075-88. 2010
  5. ncbi The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases
    Tereza Skalova
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v v i, Heyrovskeho nam 2, 162 06 Praha 6, Czech Republic
    J Mol Biol 385:1165-78. 2009
  6. pmc Diversity, abundance and expression of nitrite reductase (nirK)-like genes in marine thaumarchaea
    Marie B Lund
    Department of Environmental Earth System Science, Stanford University, Stanford, CA 94305 4216, USA
    ISME J 6:1966-77. 2012
  7. pmc Nitrite reductase NirS is required for type III secretion system expression and virulence in the human monocyte cell line THP-1 by Pseudomonas aeruginosa
    Nadine E Van Alst
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Infect Immun 77:4446-54. 2009
  8. pmc Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation
    Jack H Crawford
    Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, 35294, USA
    Blood 107:566-74. 2006
  9. ncbi Side-on copper-nitrosyl coordination by nitrite reductase
    Elitza I Tocheva
    Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Science 304:867-70. 2004
  10. ncbi The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers
    Kim Heylen
    Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Environ Microbiol 8:2012-21. 2006

Detail Information

Publications247 found, 100 shown here

  1. ncbi Bacterial nitric oxide synthesis
    F Cutruzzola
    Dipartimento di Scienze Biochimiche, Universita di Roma La Sapienza, p le A Moro, 5, 00185, Rome, Italy
    Biochim Biophys Acta 1411:231-49. 1999
    The structure-function relationships in nitrite reductases, key enzymes in the dissimilatory denitrification pathway which reduce nitrite to nitric oxide (NO), are reviewed in this paper...
  2. ncbi Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus)
    Wladyslaw Polcyn
    Department of Plant Physiology, Faculty of Biology, A Mickiewicz University, Al Niepodleglosci 14, 61 713 Poznan, Poland
    FEMS Microbiol Lett 226:331-7. 2003
    ..Lowering of growth temperature almost completely diminished nitrite accumulation and enabled consumption as high as 10 mM nitrate, which confirms such a conclusion...
  3. pmc Reciprocal regulation of cellular nitric oxide formation by nitric oxide synthase and nitrite reductases
    George B Stefano
    Neuroscience Research Institute, State University of New York College at Old Westbury, Old Westbury, NY 11568 0210, USA
    Med Sci Monit 17:RA221-6. 2011
    ..by enzymatic reduction of available cellular nitrite pools by a diverse class of cytosolic and mitochondrial nitrite reductases. Nitrite is a major metabolic product of NO and is found in all cell and tissue types that utilize NO ..
  4. ncbi Homologues of nitrite reductases in ammonia-oxidizing archaea: diversity and genomic context
    Rita Bartossek
    Department of Biology, Centre for Geobiology, University of Bergen, PO Box 7803, 5020 Bergen, Norway
    Environ Microbiol 12:1075-88. 2010
    ..Here we report on the occurrence of genes encoding potential homologues of copper-dependent nitrite reductases (NirK) in ammonia-oxidizing archaea of soils and other environments using metagenomic approaches and PCR ..
  5. ncbi The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases
    Tereza Skalova
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v v i, Heyrovskeho nam 2, 162 06 Praha 6, Czech Republic
    J Mol Biol 385:1165-78. 2009
    ..It consists of two domains and forms trimers and hence resembles the quaternary structure of nitrite reductases or ceruloplasmins more than that of large laccases...
  6. pmc Diversity, abundance and expression of nitrite reductase (nirK)-like genes in marine thaumarchaea
    Marie B Lund
    Department of Environmental Earth System Science, Stanford University, Stanford, CA 94305 4216, USA
    ISME J 6:1966-77. 2012
    ..Like the ammonia-oxidizing bacteria, AOA encode a gene homologous to copper-containing nitrite reductases (nirK), which has been studied very little to date...
  7. pmc Nitrite reductase NirS is required for type III secretion system expression and virulence in the human monocyte cell line THP-1 by Pseudomonas aeruginosa
    Nadine E Van Alst
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Infect Immun 77:4446-54. 2009
    ..These data suggest that NO generated via nitrite reductase NirS contributes to the regulation of expression of selected virulence factors in P. aeruginosa PA14...
  8. pmc Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation
    Jack H Crawford
    Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, 35294, USA
    Blood 107:566-74. 2006
    ..These data support a function for RBC hemoglobin as an allosterically and redox-regulated nitrite reductase whose "enzyme activity" couples hypoxia to increased NO-dependent blood flow...
  9. ncbi Side-on copper-nitrosyl coordination by nitrite reductase
    Elitza I Tocheva
    Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Science 304:867-70. 2004
    ..The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions...
  10. ncbi The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers
    Kim Heylen
    Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Environ Microbiol 8:2012-21. 2006
    Gene sequence analysis of nirS and nirK, both encoding nitrite reductases, was performed on cultivated denitrifiers to assess their incidence in different bacterial taxa and their taxonomical value...
  11. ncbi The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes
    J W Godden
    Department of Biochemistry, University of Washington, Seattle 98195
    Science 253:438-42. 1991
    ..The Cu-II site is at the bottom of a 12 A deep solvent channel and is the site to which the substrate (NO2-) binds, as evidenced by difference density maps of substrate-soaked and native crystals...
  12. ncbi Catalytic roles for two water bridged residues (Asp-98 and His-255) in the active site of copper-containing nitrite reductase
    M J Boulanger
    Departments of Biochemistry and Molecular Biology and of Microbiology and Immunology, University of British Columbia, Vancouver V6T 1Z3, Canada
    J Biol Chem 275:23957-64. 2000
    ..The interaction of His-255 with the bridging water appears to be necessary for catalysis and may donate a proton to reaction intermediates in addition to Asp-98...
  13. ncbi Protein film voltammetry of copper-containing nitrite reductase reveals reversible inactivation
    Hein J Wijma
    Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands
    J Am Chem Soc 129:8557-65. 2007
    ..Binding of nitrite to the type-2 site does not affect the midpoint potential of the type-1 site, thereby excluding cooperativity between the two sites...
  14. ncbi Characterization of two type 1 Cu sites of Hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases
    Kazuya Yamaguchi
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Biochemistry 43:14180-8. 2004
    ..and the 35-kDa C-terminal domain having type 1 Cu-binding and type 2 Cu-binding motifs such as common nitrite reductases (NIRs)...
  15. ncbi Identification of the proton channel to the active site type 2 Cu center of nitrite reductase: structural and enzymatic properties of the His254Phe and Asn90Ser mutants
    Michael A Hough
    Molecular Biophysics Group, School of Biological Sciences, University of Liverpool, UK
    Biochemistry 47:13547-53. 2008
    ..Two distinct proton channels have previously been identified in copper nitrite reductases based on high- to atomic-resolution crystal structures...
  16. pmc Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from the protomitochondrion?
    Sang Wan Kim
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 75:2652-8. 2009
    ..These results show that the fungal denitrifying system has the same origin as its bacterial counterpart...
  17. ncbi Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin
    Swati Basu
    Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
    Nat Chem Biol 3:785-94. 2007
    ....
  18. ncbi Atomic resolution structures of native copper nitrite reductase from Alcaligenes xylosoxidans and the active site mutant Asp92Glu
    Mark J Ellis
    Faculty of Applied Science, De Montfort University, Leicester LE1 9BH, UK
    J Mol Biol 328:429-38. 2003
    ..04, 1.15 and 1.12A resolutions, respectively. These structures provide the basis from which to build a detailed mechanism of this important enzyme...
  19. ncbi A plasma membrane-bound enzyme of tobacco roots catalyses the formation of nitric oxide from nitrite
    C Stohr
    Institut fur Botanik, Technische Universitat, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Planta 212:835-41. 2001
    ..The PM-associated NI-NOR may reduce the apoplastic nitrite produced by PM-NR in vivo and may play a role in nitrate signalling via NO formation...
  20. ncbi Structural basis of inter-protein electron transfer for nitrite reduction in denitrification
    Masaki Nojiri
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nature 462:117-20. 2009
    ....
  21. pmc Nitrite reductase activity of cytochrome c
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 283:32590-7. 2008
    ..These data suggest an additional role for cytochrome c as a nitrite reductase that may play an important role in regulating mitochondrial function and contributing to hypoxic, redox, and apoptotic signaling within the cell...
  22. ncbi Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
    Luisa B Maia
    REQUIMTE, Centro de Quimica Fina e Biotecnologia, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Campus da Caparica, 2829 516, Caparica, Portugal
    J Biol Inorg Chem 16:443-60. 2011
    ..Our results define a new catalytic performance for AOR-the nitrite reduction-and propose a new class of molybdenum-containing nitrite reductases.
  23. pmc Demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases
    Sibylle Brenner
    Manchester Interdisciplinary Biocentre and Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, United Kingdom
    J Biol Chem 284:25973-83. 2009
    ..61 +/- 0.02 between 3 and 10 mM. Our work provides the first experimental demonstration of proton-coupled electron transfer in both the resting and substrate-bound AxNiR, and two protons were found to be involved in turnover...
  24. ncbi Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil
    Zhe Chen
    Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
    Microb Ecol 60:850-61. 2010
    ..Therefore, long-term fertilization resulted in a strong impact on the community structure of nirK populations only, and total organic carbon was the dominant factor in relation to the variations of nir community sizes...
  25. ncbi Characterization of a novel copper-haem c dissimilatory nitrite reductase from Ralstonia pickettii
    Cong Han
    Molecular Biophysics Group, Institute of Integrative Biology, Faculty of Health and Life Sciences, University of Liverpool, UK
    Biochem J 444:219-26. 2012
    NiRs (nitrite reductases) convert nitrite into NO in the denitrification process. RpNiR (Ralstonia pickettii NiR), a new type of dissimilatory Cu-containing NiR with a C-terminal haem c domain from R...
  26. pmc Structure and function of a hexameric copper-containing nitrite reductase
    Masaki Nojiri
    Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560 0043, Japan
    Proc Natl Acad Sci U S A 104:4315-20. 2007
    ..The discussion of the intermolecular electron transfer process from cytochrome c(550) to the H. denitrificans NIR is based on x-ray crystallographic and kinetic results...
  27. pmc Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism
    Svetlana V Antonyuk
    Molecular Biophysics Group, Council for the Central Laboratory of the Research Councils Daresbury Laboratory, Warrington WA4 4AD, United Kingdom
    Proc Natl Acad Sci U S A 102:12041-6. 2005
    Copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (NO), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere...
  28. ncbi Binding and activation of nitrite and nitric oxide by copper nitrite reductase and corresponding model complexes
    Anna C Merkle
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Dalton Trans 41:3355-68. 2012
    The reduction of nitrite to nitric oxide in dissimilatory denitrification is carried out by copper nitrite reductases (CuNIRs) via a type 2 copper site...
  29. pmc Quantification of nitrogen reductase and nitrite reductase genes in soil of thinned and clear-cut Douglas-fir stands by using real-time PCR
    David J Levy-Booth
    Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 W Burnside Rd, Victoria, British Columbia V8Z 1M5, Canada
    Appl Environ Microbiol 76:7116-25. 2010
    ..Overall, no clear influence of tree thinning on nifH, nirS, and nirK was observed. However, soil OM, C, and N were found to significantly influence N-cycling gene abundance...
  30. ncbi Active site comparison of CoII blue and green nitrite reductases
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Chemistry 12:6647-59. 2006
    Copper-containing nitrite reductases (NiRs) possess type 1 (T1) and type 2 (T2) copper sites and can be either green or blue in color owing to differences at their T1 centers...
  31. ncbi Intramolecular electron transfer in nitrite reductases
    Scot Wherland
    Department of Chemistry, Washington State University, Pullman, WA 99164 4630, USA
    Chemphyschem 6:805-12. 2005
    The copper- and heme-containing nitrite reductases (NiRs) are key enzymes in denitrification...
  32. ncbi Anammox bacteria disguised as denitrifiers: nitrate reduction to dinitrogen gas via nitrite and ammonium
    Boran Kartal
    Radboud University Nijmegen, Institute for Water and Wetland Research, Department of Microbiology, Nijmegen, 6525 ED, The Netherlands
    Environ Microbiol 9:635-42. 2007
    ..This indicates that anammox bacteria could be mediating dissimilatory nitrate reduction to ammonium in natural ecosystems...
  33. ncbi Deoxymyoglobin is a nitrite reductase that generates nitric oxide and regulates mitochondrial respiration
    Sruti Shiva
    Vascular Medicine Branch, Clinical Center National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circ Res 100:654-61. 2007
    ....
  34. ncbi Molecular diversity of nitrite reductase genes (nirK) in nitrifying bacteria
    J Jason L Cantera
    Department of Environmental Sciences, Geology 2207, University of California, Riverside, CA 92521, USA
    Environ Microbiol 9:765-76. 2007
    ..Deduced NirK protein sequences were highly similar to other copper-containing nitrite reductases including conserved motifs...
  35. ncbi Denitrification and ammonia oxidation by Nitrosomonas europaea wild-type, and NirK- and NorB-deficient mutants
    Ingo Schmidt
    Department of Microbiology, University of Bayreuth, Universitaetsstrasse 30, 95447 Bayreuth, Germany
    Microbiology 150:4107-14. 2004
    ..In contrast, the mutant strains remained inactive. For recovery of ammonia oxidation activity the NO concentration had to be adjusted to about 10 p.p.m. in the aeration gas...
  36. ncbi Formation and possible roles of nitric oxide in plant roots
    Christine Stöhr
    Institut fur Botanik, Ernst Moritz Arndt Universitat, Grimmer Strasse 88, D 17487 Greifswald, Germany
    J Exp Bot 57:463-70. 2006
    ....
  37. pmc The nrfA and nirB nitrite reductase operons in Escherichia coli are expressed differently in response to nitrate than to nitrite
    H Wang
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles, California 90095 1489, USA
    J Bacteriol 182:5813-22. 2000
    ..In contrast, the nirB nitrite reductase is optimally synthesized only when nitrate or nitrite is in excess of the cell's capacity to consume it. Revised regulatory schemes are presented for NarL and NarP in control of the two operons...
  38. pmc The detection of the nitrite reductase and NO-generating properties of haemoglobin by mitochondrial inhibition
    Sruti Shiva
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA
    Cardiovasc Res 89:566-73. 2011
    ..Here, we characterize the NO-generating properties of Hb using the cGMP-independent and NO-dependent inhibition of mitochondrial cytochrome c oxidase...
  39. pmc The functional nitrite reductase activity of the heme-globins
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
    Blood 112:2636-47. 2008
    ..We will review these reactions in the biologic framework of hypoxic signaling in blood and the heart...
  40. ncbi Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:12916-27. 2007
    ....
  41. ncbi Nitric oxide formation by Escherichia coli. Dependence on nitrite reductase, the NO-sensing regulator Fnr, and flavohemoglobin Hmp
    Hazel Corker
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 278:31584-92. 2003
    ..A cytochrome bd-deficient mutant retained NO-generating activity. The present study reveals a critical balance between NO-generating and -detoxifying activities during anaerobic growth...
  42. ncbi The emerging role of nitrite as an endogenous modulator and therapeutic agent of cardiovascular function
    B Tota
    Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rende, CS, Italy
    Curr Med Chem 17:1915-25. 2010
    ....
  43. ncbi The existence and significance of a mitochondrial nitrite reductase
    Hans Nohl
    Research Institute for Biochemical Pharmacology and Toxicology, University of Veterinary Medicine of Vienna, Vienna, Austria
    Redox Rep 10:281-6. 2005
    ..In this paper, we describe the identity and the function of mitochondrial nitrite reductase and the consequences of NO-recycling in the metabolic compartment of mitochondria...
  44. ncbi Optical biosensor based on nitrite reductase immobilised in controlled pore glass
    Carla C Rosa
    Biosensors Laboratory, IBET ITQB Instituto de Biologia Experimental e Tecnológica Instituto de Tecnologia Química e Biológica, Apartado 12, P 2781 901 Oeiras, Portugal
    Biosens Bioelectron 17:45-52. 2002
    ..The corresponding detection limit of 0.93 microM is well below the maximum admissible concentration imposed by European Community norms, of 2.2 microM...
  45. ncbi X-ray structure and site-directed mutagenesis of a nitrite reductase from Alcaligenes faecalis S-6: roles of two copper atoms in nitrite reduction
    M Kukimoto
    Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan
    Biochemistry 33:5246-52. 1994
    ..Kinetic analysis of the electron transfer between NIR and pseudoazurin revealed that the electron-transfer rate between Met150Glu and pseudoazurin was reduced 1000-fold relative to that of wild-type NIR.(ABSTRACT TRUNCATED AT 250 WORDS)..
  46. ncbi Structure-based engineering of Alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin
    Kunishige Kataoka
    Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920 1192, Japan
    J Biol Chem 279:53374-8. 2004
    ..from Achromobacter cycloclastes pseudoazurin (AcPAZ) to wild-type and mutant Alcaligenes xylosoxidans nitrite reductases (AxNIRs) was investigated using steady-state kinetics and electrochemical methods...
  47. ncbi Electrochemical characterization of biosensor based on nitrite reductase and methyl viologen co-immobilized glassy carbon electrode
    De Quan
    The Chemical Sensor Research Group, Department of Chemistry, Kwangwoon University, Seoul 139 701, Republic of Korea
    Bioelectrochemistry 69:267-75. 2006
    ..In addition to the full characterization of the biosensor, kinetic study was also conducted in solution and the homogeneous rate constant (k(2)) between NiR and MV were determined by chronoamperometry to be 5.8 x 10(5) M(-1) s(-1)...
  48. ncbi Biosensing nitrite using the system nitrite redutase/Nafion/methyl viologen--a voltammetric study
    M Gabriela Almeida
    REQUIMTE, Departamento de Quimica, Centro de Quimica Fina e Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Monte de Caparica, Portugal
    Biosens Bioelectron 22:2485-92. 2007
    ..The operational stability of the biosensor and the influence of some possible interferences were evaluated...
  49. ncbi Application of nitrite reductase from Alcaligenes faecalis S-6 for nitrite measurement
    S Sasaki
    Research Center for Advanced Science and Technology, University of Tokyo, Japan
    Biosens Bioelectron 13:1-5. 1998
    ..Measurements using a batch-flow type system gave a lower detection limit of 0.01 mg/l. This is sufficient for the detection of nitrite in natural waters...
  50. ncbi Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications
    M E Murphy
    Department of Biological Structure, School of Medicine, University of Washington, Seattle, Washington 98195 7420, USA
    J Biol Chem 272:28455-60. 1997
    ....
  51. ncbi Sensing nitrite through a pseudoazurin-nitrite reductase electron transfer relay
    Yann Astier
    Inorganic Chemistry Laboratory, University of Oxford, UK
    Chemphyschem 6:1114-20. 2005
    ....
  52. ncbi Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135
    Dora Pinho
    REQUIMTE, Departamento de Quimica, Faculdade de Ciencias, Universidade do Porto, Portugal
    Eur J Biochem 271:2361-9. 2004
    ..Electrochemical experiments show that azurin from the same organism can donate electrons to this enzyme...
  53. ncbi Genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases
    Mark J Ellis
    Molecular Biophysics Group, Science and Technology Facilities Council, Daresbury Laboratory, Warrington, Cheshire, UK
    J Biol Inorg Chem 12:1119-27. 2007
    ....
  54. ncbi The activity of the high-affinity nitrate transport system I (NRT2;1, NAR2) is responsible for the efficient signalling of nitrate assimilation genes in Chlamydomonas reinhardtii
    Jesús Rexach
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cordoba, Campus de Rabanales, Edif Severo Ochoa, 14071 Cordoba, Spain
    Planta 215:606-11. 2002
    ..The bispecific nitrate/nitrite transport system I with the highest affinity for nitrate is the most efficient one for signalling the expression of nitrate/nitrite assimilation genes in C. reinhardtii...
  55. ncbi Nitrate reductase and nitrite reductase transcript levels in various mutants of Aspergillus nidulans: confirmation of autogenous regulation
    K L Hawker
    Plant Molecular Genetics Unit, University of St Andrews, Fife, UK
    Mol Gen Genet 231:485-8. 1992
    ....
  56. ncbi Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
    Kenyatta Cosby
    Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
    Nat Med 9:1498-505. 2003
    ..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation...
  57. pmc The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter
    M I Muro-Pastor
    Institut de Genetique et Microbiologie, Universite Paris Sud, UMR CNRS C8621, 91405 Orsay Cedex, France
    EMBO J 18:1584-97. 1999
    ..The results presented cast doubts on the role of NirA as the unique transducer of the nitrate induction signal. We demonstrate, for the first time in vivo, that a GATA factor is involved directly in chromatin remodelling...
  58. ncbi Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor
    Hubertus J E Beaumont
    BioCentrum Amsterdam, Department of Molecular Cell Physiology, Vrije Universiteit, De Boelelaan 1087, NL 1081 HV Amsterdam, The Netherlands
    Mol Microbiol 54:148-58. 2004
    ..The findings are consistent with the hypothesis that N. europaea expresses NirK as a defence against the toxic NO(2) (-) that is produced during nitrification...
  59. pmc A paradoxical mutant GATA factor
    M Isabel Muro-Pastor
    Institut de Genetique et Microbiologie, Universite Paris Sud, UMR8621, 91405 Orsay Cedex, France
    Eukaryot Cell 3:393-405. 2004
    ..The in vivo methylation pattern of the promoter is drastically altered, suggesting the recruitment of one or more unknown transcription factors and/or a local distortion on the DNA double helix...
  60. ncbi Catalytic function and local proton structure at the type 2 copper of nitrite reductase: the correlation of enzymatic pH dependence, conserved residues, and proton hyperfine structure
    Yiwei Zhao
    Department of Chemistry, Center for Biophysics and Biochemistry, University at Albany, SUNY, Albany, NY 12222, USA
    Biochemistry 41:7464-74. 2002
    ..Mutation of Ile289 to Ala and Val caused minor perturbation to enzyme activity, but as detected by ENDOR, allowed formate binding. Thus, bulky Ile289 may exclude non-nitrite ligands from the Type 2 active site...
  61. ncbi Conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules
    Elitza I Tocheva
    Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver BC, V6T 1Z3, Canada
    Biochemistry 47:4452-60. 2008
    ..Overall, the data substantiate the roles of Asp98 and Ile257 in discriminating substrate from other small anions...
  62. ncbi Calorimetric and spectroscopic investigations of the thermal denaturation of wild type nitrite reductase
    Andrea Stirpe
    Dipartimento di Fisica e Unità INFM, Laboratorio di Biofisica Molecolare, Universita della Calabria, Ponte P Bucci Cubo 31C, I 87036, Arcavacata di Rende CS, Italy
    Biochim Biophys Acta 1752:47-55. 2005
    ..Moreover, it is found that the conformational changes within the type-1 copper environments precede the denaturation of the whole protein. No evidence of protein dissociation within the temperature range investigated was observed...
  63. ncbi Opposing signals differentially regulate transcript stability in Aspergillus nidulans
    Mark X Caddick
    The University of Liverpool, School of Biological Sciences, Biosciences Building, Crown Street, Liverpool, L69 7ZB, UK
    Mol Microbiol 62:509-19. 2006
    ..Regulated transcript stability is therefore an integral part of the adaptive response. This represents the first example of distinct physiological signals competing to differentially regulate transcripts at the level of deadenylation...
  64. pmc Purification, characterization, and genetic analysis of Cu-containing dissimilatory nitrite reductase from a denitrifying halophilic archaeon, Haloarcula marismortui
    H Ichiki
    Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Shizuoka 422 8529, Japan
    J Bacteriol 183:4149-56. 2001
    ....
  65. ncbi Metal coordination and mechanism of multicopper nitrite reductase
    S Suzuki
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Acc Chem Res 33:728-35. 2000
    ..The noncoordinated Asp and His residues around type 2 Cu play important roles in both the electron-transfer and the catalytic processes...
  66. ncbi Observation of fast release of NO from ferrous d₁ haem allows formulation of a unified reaction mechanism for cytochrome cd₁ nitrite reductases
    Serena Rinaldo
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Sapienza Universita di Roma, P A Moro, 5 00185 Rome, Italy
    Biochem J 435:217-25. 2011
    ..We present a reaction mechanism proposed to be applicable to all cytochromes cd1 and conclude that the d1 haem has evolved to have low affinity for NO, as compared with other ferrous haems...
  67. ncbi Electron transport through nitrate and nitrite reductases in Campylobacter jejuni
    M S Pittman
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 33:190-2. 2005
    ..The pathways of electron transport to nitrate and nitrite are reviewed in this paper. Nitrate is considered as a potential in vivo electron acceptor and a role for nitrite reductase in NO detoxification is suggested...
  68. ncbi Nitrite-dependent vasodilation is facilitated by hypoxia and is independent of known NO-generating nitrite reductase activities
    Thomas Dalsgaard
    Department of Biological Sciences, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Heart Circ Physiol 292:H3072-8. 2007
    ..Possible reaction mechanisms for nitrite vasoactivity, including formation of S-nitrosothiols within the arterial smooth muscle, are discussed...
  69. ncbi Electron donation between copper containing nitrite reductases and cupredoxins: the nature of protein-protein interaction in complex formation
    Loretta M Murphy
    CLRC Daresbury Laboratory, Warrington, Cheshire, UK
    J Mol Biol 315:859-71. 2002
    In denitrifying organisms with copper containing dissimilatory nitrite reductases, electron donation from a reduced cupredoxin is an essential step in the reduction of nitrite to nitric oxide...
  70. pmc Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland
    Ellen Kandeler
    Institute of Soil Science, University of Hohenheim, D 70593 Stuttgart, Germany
    Appl Environ Microbiol 72:5957-62. 2006
    ..This study yields an initial insight into the ecology of bacteria carrying genes for the denitrification pathway in a newly developing alpine environment...
  71. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  72. pmc Nitrate/nitrite assimilation system of the marine picoplanktonic cyanobacterium Synechococcus sp. strain WH 8103: effect of nitrogen source and availability on gene expression
    Clare Bird
    School of Biological and Environmental Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom
    Appl Environ Microbiol 69:7009-18. 2003
    ..The ecological implications of these findings are discussed in the context of the oligotrophic nature of oceanic surface waters in which Synechococcus spp. thrive...
  73. ncbi Very early reaction intermediates detected by microsecond time scale kinetics of cytochrome cd1-catalyzed reduction of nitrite
    Katharine A Sam
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 283:27403-9. 2008
    ..Spectroscopic observation of the subsequent transformation of this species has provided data that demand reappraisal of the general belief that the two subunits of the enzyme function independently...
  74. ncbi Coordinate synthesis of azurin I and copper nitrite reductase in Alcaligenes xylosoxidans during denitrification
    Roger L Harris
    Department of Molecular Microbiology, John Innes Centre, Norwich, UK
    Arch Microbiol 186:241-9. 2006
    ..Taken together, these findings indicate that in A. xylosoxidans azuA expression is coordinated with denitrification and recombinant Az I is processed and matured in the periplasm of E. coli in the same way it is in A. xylosoxidans...
  75. ncbi Organization of the respiratory chain of Neisseria meningitidis
    M Deeudom
    Department of Biology Area 10, University of York, Heslington, York YO10 5YW, UK
    Biochem Soc Trans 34:139-42. 2006
    ..Nitrite inhibits the oxidation of cytochromes by oxygen in a nitrite reductase-independent manner, indicating that nitrite may inhibit oxidase activity directly, as well as via the intermediate of denitrification, nitric oxide...
  76. pmc Signal transduction protein PII phosphatase PphA is required for light-dependent control of nitrate utilization in synechocystis sp. strain PCC 6803
    Nicole Kloft
    Institut fur Mikrobiologie und Molekularbiologie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 26 32, D 35392 Giessen, Germany
    J Bacteriol 187:6683-90. 2005
    ..Uncontrolled nitrate uptake leads to light-dependent nitrite excretion even in wild-type cells, confirming that nitrate uptake controls nitrate utilization in response to limiting photon flux densities...
  77. ncbi Investigation into the role of the truncated denitrification chain in Rhizobium sullae strain HCNT1
    S Casella
    Dipartimento di Biotecnologie Agrarie, University of Padova, Agripolis, Viale dell Universita 16, 35020 Legnaro, Padova, Italy
    Biochem Soc Trans 34:130-2. 2006
    ..sullae strains and may provide an explanation as to why HCNT1 possesses this unusual truncation of its denitrification electron transport chain...
  78. ncbi Biophysics. Catching copper in the act
    Nermeen W Aboelella
    Department of Chemistry and Center for Metals in Biocatalysis, University of Minnesota, Minneapolis, MN 55455, USA
    Science 304:836-7. 2004
  79. ncbi Experimental evidence for plasmid-borne nor-nir genes in Sinorhizobium meliloti JJ1c10
    Yiu Kwok Chan
    Eastern Cereal and Oilseed Research Centre, Agriculture and Agri Food Canada, Ottawa, ON
    Can J Microbiol 50:657-67. 2004
    ..This relationship may be due to lateral gene transfer of denitrification genes from a common donor followed by rearrangement and recombination of these genes...
  80. pmc Mechanisms underlying erythrocyte and endothelial nitrite reduction to nitric oxide in hypoxia: role for xanthine oxidoreductase and endothelial nitric oxide synthase
    Andrew J Webb
    William Harvey Research Institute, Centre for Clinical Pharmacology, Barts and The London, Charterhouse Square, London, UK
    Circ Res 103:957-64. 2008
    ....
  81. ncbi Bidirectional catalysis by copper-containing nitrite reductase
    Hein J Wijma
    Leiden Institute of Chemistry, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochemistry 43:10467-74. 2004
    ..At pH 8 the K(eq) amounts to 10(3). The results show that dissimilatory nitrite reductases catalyze an unfavorable reaction at physiological pH (pH = 7-8)...
  82. ncbi Evidence for nitrite reductase activity in intact mouse Leydig tumor cells
    N S Panesar
    Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, PR China
    Steroids 71:984-92. 2006
    ..In conclusion, this study supports the existence of a surrogate mammalian nitrite reductase and the dormancy of L-arginine-NOS pathway in MLTC-1...
  83. pmc Differences in nitric oxide steady states between arginine, hypoxanthine, uracil auxotrophs (AHU) and non-AHU strains of Neisseria gonorrhoeae during anaerobic respiration in the presence of nitrite
    Kenneth Barth
    Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Can J Microbiol 54:639-46. 2008
    ....
  84. pmc Biphasic behavior of anammox regulated by nitrite and nitrate in an estuarine sediment
    Mark Trimmer
    School of Biological Sciences, Queen Mary, University of London, London E1 4NS, United Kingdom
    Appl Environ Microbiol 71:1923-30. 2005
    ..Overall, anammox is likely to be regulated by the availability of NO3- and NO2- and the relative size or activity of the anammox population...
  85. ncbi Linkage isomerism in nitrite reduction by cytochrome cd1 nitrite reductase
    Radu Silaghi-Dumitrescu
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Inorg Chem 43:3715-8. 2004
    ..O-Coordination would offer an advantage since the end product of nitrite reduction would be a ferric-hydroxo/water complex, rather than the more kinetically inert iron-nitrosyl complex implied by the N-nitrite mechanism...
  86. ncbi Pseudoazurin dramatically enhances the reaction profile of nitrite reduction by Paracoccus pantotrophus cytochrome cd1 and facilitates release of product nitric oxide
    Katharine A Sam
    Department of Biochemistry, University of Oxford South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 283:12555-63. 2008
    ..It is clear that cytochrome cd(1) functions significantly differently in the presence of its two substrates, nitrite and electron donor protein, than in the presence of nitrite alone...
  87. ncbi Unexpected dependence on pH of NO release from Paracoccus pantotrophus cytochrome cd1
    Katharine A Sam
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Biochem Biophys Res Commun 371:719-23. 2008
    ..The kinetically stable product formed at the end of the reaction differs significantly at pH 6.0 and 7.0, as does its rate of formation; thus the reaction is critically dependent on pH...
  88. ncbi The pathogen Neisseria meningitidis requires oxygen, but supplements growth by denitrification. Nitrite, nitric oxide and oxygen control respiratory flux at genetic and metabolic levels
    Jonathan D Rock
    Department of Biology Area 10, University of York, Heslington, York YO10 5YW, UK
    Mol Microbiol 58:800-9. 2005
    ..meningitidis...
  89. ncbi Denitrification of nitrate by the fungus Cylindrocarpon tonkinense
    Tomo o Watsuji
    Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Biosci Biotechnol Biochem 67:1115-20. 2003
    ..The denitrification was inhibited by tungstate, an inhibitor of Nar. These results demonstrated occurrence of a novel type of denitrification in C. tonkinense, in which assimilatory type Nar is possibly involved...
  90. pmc Catalytic and spectroscopic analysis of blue copper-containing nitrite reductase mutants altered in the environment of the type 2 copper centre: implications for substrate interaction
    M Prudencio
    Department of Biological Chemistry, John Innes Centre, Colney Lane, Norwich NR4 7UH, U K
    Biochem J 353:259-66. 2001
    ..These findings suggest that on formation of an electron transfer complex with azurin, a conformational change in NiR occurs that returns the catalytic Cu centre to a functionally active state capable of binding and reducing nitrite...
  91. pmc Cytochromes c(550), c(552), and c(1) in the electron transport network of Paracoccus denitrificans: redundant or subtly different in function?
    M F Otten
    Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Free University, 1081 HV Amsterdam, The Netherlands
    J Bacteriol 183:7017-26. 2001
    ....
  92. ncbi Two c-type cytochromes, NirM and NirC, encoded in the nir gene cluster of Pseudomonas aeruginosa act as electron donors for nitrite reductase
    N Hasegawa
    Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan
    Biochem Biophys Res Commun 288:1223-30. 2001
    ..NirC, as well as NirM, was able to mediate the electron donation from the membrane electron pathway to NIR, suggesting that the structural gene for NIR is followed by the genes for two electron donors for NIR...
  93. ncbi Alternate substrate binding modes to two mutant (D98N and H255N) forms of nitrite reductase from Alcaligenes faecalis S-6: structural model of a transient catalytic intermediate
    M J Boulanger
    Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Biochemistry 40:9132-41. 2001
    ..The formation of an unusual dinuclear type I copper site in the reduced nitrite soaked D98N and H255N crystal structures may represent an evolutionary link between the mononuclear type I copper centers and dinuclear Cu(A) sites...
  94. ncbi A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria
    H Hussain
    School of Biochemistry, University of Birmingham, UK
    Mol Microbiol 12:153-63. 1994
    ..The sequence of the 127 residue NrfF polypeptide, M(r) 14,522, is strikingly similar to the CcI2 protein of R. capsulatus, especially in the putative haem-binding motif, RCPQCQNQN.(ABSTRACT TRUNCATED AT 400 WORDS)..
  95. pmc Nitric oxide signaling and transcriptional control of denitrification genes in Pseudomonas stutzeri
    K U Vollack
    , D-76128 Karlsruhe, Germany
    J Bacteriol 183:2516-26. 2001
    ..stutzeri. The NO response adds to the previously identified nitrate-nitrite response mediated by the NarXL two-component system for the expression of respiratory nitrate reductase encoded by the narGHJI operon...
  96. ncbi Mechanism of nitrite reduction at T2Cu centers: electronic structure calculations of catalysis by copper nitrite reductase and by synthetic model compounds
    Mahesh Sundararajan
    School of Chemistry, University of Manchester, Manchester, M13 9PL, United Kingdom
    J Phys Chem B 111:5511-7. 2007
    ..Further protonation of this species leads to N-O bond cleavage, and an electron transfer from the Cu(I) center to the N-O+ ligand, resulting in loss of NO and regeneration of the resting state of the enzyme having a bound water molecule...
  97. ncbi Role of nitrite reductase in the ammonia-oxidizing pathway of Nitrosomonas europaea
    J Jason L Cantera
    Department of Environmental Sciences, Geology 2207, University of California, Riverside, CA 92521, USA
    Arch Microbiol 188:349-54. 2007
    ..europaea indicated the presence of an alternative, enzymatic pathway for N(2)O production...
  98. ncbi pH dependence of copper geometry, reduction potential, and nitrite affinity in nitrite reductase
    Frida Jacobson
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Box 462, SE 40530 Goteborg, Sweden
    J Biol Chem 282:6347-55. 2007
    ..Taken together, these spectroscopic and structural observations help to explain the pH dependence of nitrite reductase, highlighting the subtle relationship between copper site geometry, nitrite affinity, and enzyme activity...
  99. ncbi Role of Bradyrhizobium japonicum cytochrome c550 in nitrite and nitrate respiration
    Emilio Bueno
    Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Profesor Albareda 1, Granada, Spain
    FEMS Microbiol Lett 279:188-94. 2008
    ....
  100. pmc Nitrate assimilation genes of the marine diazotrophic, filamentous cyanobacterium Trichodesmium sp. strain WH9601
    Q Wang
    H Steinitz Marine Biology Laboratory, Hebrew University, Coral Beach, 88103 Eilat, Israel
    J Bacteriol 182:1764-7. 2000
    ..NapA was involved in both nitrate and nitrite uptake by the complemented B. subtilis cells. napA is distinct from the nrt genes that encode the nitrate transporter of freshwater cyanobacteria...
  101. pmc The requirement of RpoN (sigma factor sigma54) in denitrification by Pseudomonas stutzeri is indirect and restricted to the reduction of nitrite and nitric oxide
    E Hartig
    Lehrstuhl für Mikrobiologie der Universität Karlsruhe, D 76128 Karlsruhe, Germany
    Appl Environ Microbiol 64:3092-5. 1998
    ....

Research Grants31

  1. BIOENGINEERING DESIGN OF ARTIFICIAL BLOOD
    Marcos Intaglietta; Fiscal Year: 2011
    ..abstract_text> ..
  2. Myoglobin as a Nitrite Reductase that Regulates Hypoxic Cardiac NO Signaling
    Sruti Shiva; Fiscal Year: 2013
    ..Analogous to the bacterial nitrite reductases, a concerted proton and electron transfer reaction to nitrite reduces the anion to NO...
  3. Cell Free O2 Carriers: Cerebrovascular Control and Stroke
    RAYMOND CHARLES KOEHLER; Fiscal Year: 2013
    ....
  4. Role of hemoglobin b93cys residue in nitric oxide bioactivity
    RAKESH PRAVINCHANDRA PATEL; Fiscal Year: 2013
    ....
  5. Multiscale, Transport-Dependent NO Signaling: Cells to Vascular Networks
    Dov Jaron; Fiscal Year: 2013
    ..The proposed model will lead to an improved understanding of the complete system, and set the groundwork for future research that can shed light on NO-related pathologies. ..
  6. Study of copper oxidoreductases through metalloprotein engineering
    Steven M Berry; Fiscal Year: 2012
    ..Defining the features that lead to activity of copper in these enzymes can contribute to an understanding of factors underlying various diseases. ..
  7. Production of Bioactive NO: Origin of Hemoglobin E Associated Pathophysiology
    RHODA HIRSCH; Fiscal Year: 2012
    ..There is the potential for a new paradigm in which to develop therapies for HbE [unreadable]-thalassemia. ..
  8. RBC age and potentiation of transfusion related pathology in trauma patients
    JORDAN ALLAN WEINBERG; Fiscal Year: 2012
    ..We anticipate that elucidation of these mechanisms will improve our understanding of RBC functions in transfusions and allow the design of therapies to limit any toxic effects. ..
  9. Nitrogen Oxides and Heme
    GEORGE RICHTER ADDO; Fiscal Year: 2007
    ..Interestingly, myoglobin (Mb) and hemoglobin have recently been proposed to act as nitrite reductases under hypoxic conditions, generating NO in the oxygen-deficient environment...
  10. TRANSITION METAL ORGANOIMIDO PORPHYRINS
    JOANNE SMIEJA; Fiscal Year: 1990
    ....
  11. Polynuclear Clusters in Biology - Structure, Reactivity
    Dimitri Coucouvanis; Fiscal Year: 2005
    ..to ammonia in nitrogenase, the 6e-reduction of sulfite to sulfide and of nitrite to ammonia in sulfite and nitrite reductases respective And the 4e- oxidation of 2H20 to O2 in photosynthesis...
  12. FUNCTION OF GONOCOCCAL ANAEROBICALLY INDUCED PROTEINS
    Virginia Clark; Fiscal Year: 2007
    ..In addition, significant new insights into the mechanism of gonococcal suppression of the innate immune response may be attained. [unreadable] [unreadable]..
  13. EPR AND ENDOR OF PARAMAGNETIC BIOMOLECULES
    CHARLES SCHOLES; Fiscal Year: 2002
    ..The plan of this work is to uncover the liganding environment at and near the 6th heme ligand of the binuclear oxygen-consuming center of bo oxidase. ..
  14. Polynuclear Clusters in Nitrogenase-Structure Reactivity
    Dimitri Coucouvanis; Fiscal Year: 2007
    ..N2 to ammonia in nitrogenase, the 6ereduction of sulfite to sulfide and of nitrite to ammonia in sulfite and nitrite reductases respectively and the 4eoxidation of 2H2O to O2 in photosynthesis...
  15. AREA DETECTOR-ROTATING ANODE FOR PROTEIN CRYSTALLOGRAPHY
    Jung Ja Kim; Fiscal Year: 1992
    ..This facility will allow data to be collected in house at the Medical College of Wisconsin, at a high rate and permits us to expedite the progress and broaden the scope of above projects...
  16. EPR AND ENDOR OF PARAMAGNETIC BIOMOLECULES
    CHARLES SCHOLES; Fiscal Year: 2006
    ..abstract_text> ..
  17. DIOXO-ISOBACTERIOCHLORIN AND CD-TYPE CYTOCHROMES
    Chi Chang; Fiscal Year: 1993
    ....
  18. Flavonoid Modulation of Epithelial Migration and Signal*
    NORMAN HORD; Fiscal Year: 2004
    ..Ultimately, this research will provide critical information necessary for specific and rational dietary recommendations to decrease colon cancer risk in humans. ..
  19. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2006
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  20. Multifunctional Nanoparticles for Intracellular Delivery
    Stefan Franzen; Fiscal Year: 2006
    ..5. Develop strategies for covalent attachment of proteins and oligonucleotides to nanoparticles. 6. Determine of the efficiency of regulated in vitro protein expression using a well-characterized model system. ..
  21. HYPOXIC PULMONARY VASOCANSTRICTION AND RED BLOOD CELLS
    Steven Deem; Fiscal Year: 2002
    ....
  22. IRON, FUR AND ALGINATE PRODUCTION IN P AERUGINOSA
    DANIEL HASSETT; Fiscal Year: 2002
    ..Information derived from the studies proposed herein will advance the current knowledge about the role of these factors in the pathogenesis of P. aeruginosa infections in CF. ..
  23. OXYGEN SENSING AND REGULATION OF YEAST GENES
    ROBERT POYTON; Fiscal Year: 2002
    ..some other redox-sensitive oxygen binding protein involved in OCD gene expression? Aim 3: Does oxygen affect transcript turnover rate? Aim 4: What cis promoter sites mediate the OCD expression of COX6, COX5a, and CYC1? ..
  24. Proteogenome of Anaerobic P. aeruginosa in CF Mucus
    DANIEL HASSETT; Fiscal Year: 2003
    ..Our LONG TERM GOAL is to identify cellular targets that inhibit or significantly compromise the denitrification process that is occurring by P. aeruginosa in the CF airways. ..
  25. B.pseudomallei bioterrorism and quorum sensing
    DANIEL HASSETT; Fiscal Year: 2003
    ..These studies will provide important information that will lead to countermeasures against a potential bioterrorist attack using the potentially lethal gram-negative bacterium, B. pseudomallei. ..
  26. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  27. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  28. Red Blood Cells, Nitric Oxide and Pulmonary Circulation
    Steven Deem; Fiscal Year: 2008
    ..abstract_text> ..
  29. Old Westbury Neuroscience Research Institute MIDARP
    George Stefano; Fiscal Year: 2008
    ..abstract_text> ..
  30. Role of OxyR in P. aeruginosa Biofilm Resistance to H202
    DANIEL HASSETT; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  31. Mitchondria-Nuclear Crosstalk in Hypoxic Gene Induction
    ROBERT POYTON; Fiscal Year: 2009
    ..g., anemia, myocardial infarction, retinopathy, and the growth of tumors). An understanding of hypoxic signaling pathways may lead to new therapies for these diseases and may help with our understanding of human aging. ..