quinone reductases

Summary

Summary: NAD(P)H:(quinone acceptor) oxidoreductases. A family that includes three enzymes which are distinguished by their sensitivity to various inhibitors. EC 1.6.99.2 (NAD(P)H DEHYDROGENASE (QUINONE);) is a flavoprotein which reduces various quinones in the presence of NADH or NADPH and is inhibited by dicoumarol. EC 1.6.99.5 (NADH dehydrogenase (quinone)) requires NADH, is inhibited by AMP and 2,4-dinitrophenol but not by dicoumarol or folic acid derivatives. EC 1.6.99.6 (NADPH dehydrogenase (quinone)) requires NADPH and is inhibited by dicoumarol and folic acid derivatives but not by 2,4-dinitrophenol.

Top Publications

  1. ncbi The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked
    Takao Yagi
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:2266-74. 2003
  2. pmc The structure of the leukemia drug imatinib bound to human quinone reductase 2 (NQO2)
    Jonathan A Winger
    Department of Molecular and Cell Biology, California Institute for Quantitative Biosciences, Howard Hughes Medical Institute, University of California, Berkeley, USA
    BMC Struct Biol 9:7. 2009
  3. ncbi Quantitative chemical proteomics reveals mechanisms of action of clinical ABL kinase inhibitors
    Marcus Bantscheff
    Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 25:1035-44. 2007
  4. pmc Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1
    G Asher
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 98:1188-93. 2001
  5. ncbi Two minor NQO1 and NQO2 alleles predict poor response of breast cancer patients to adjuvant doxorubicin and cyclophosphamide therapy
    David Jamieson
    Northern Institute for Cancer Research, Paul O Gorman Building, Medical School, Newcastle University, Newcastle upon Tyne, UK
    Pharmacogenet Genomics 21:808-19. 2011
  6. ncbi New roles of flavoproteins in molecular cell biology: an unexpected role for quinone reductases as regulators of proteasomal degradation
    Sonja Sollner
    Technische Universitat Graz, Institut fur Biochemie, Austria
    FEBS J 276:4313-24. 2009
  7. ncbi Superoxide dismutase and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase polymorphisms and pancreatic cancer risk
    Beatrice Mohelnikova-Duchonova
    Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic
    Pancreas 40:72-8. 2011
  8. pmc Crystal structure of quinone reductase 2 in complex with resveratrol
    Leonid Buryanovskyy
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Biochemistry 43:11417-26. 2004
  9. ncbi Association of superoxide dismutases and NAD(P)H quinone oxidoreductases with prognosis of patients with breast carcinomas
    Miluse Hubackova
    Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic
    Int J Cancer 130:338-48. 2012
  10. ncbi Insights into the redox cycle of human quinone reductase 2
    Karine Reybier
    Université de Toulouse 3, UPS, UMR 152 PHARMA DEV, 118 route de Narbonne, F 31062 Toulouse Cedex 9, France
    Free Radic Res 45:1184-95. 2011

Research Grants

Detail Information

Publications193 found, 100 shown here

  1. ncbi The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked
    Takao Yagi
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:2266-74. 2003
  2. pmc The structure of the leukemia drug imatinib bound to human quinone reductase 2 (NQO2)
    Jonathan A Winger
    Department of Molecular and Cell Biology, California Institute for Quantitative Biosciences, Howard Hughes Medical Institute, University of California, Berkeley, USA
    BMC Struct Biol 9:7. 2009
    ..Recent screens carried out to find off-target proteins that bind to imatinib identified the oxidoreductase NQO2, a flavoprotein that is phosphorylated in a chronic myelogenous leukemia cell line...
  3. ncbi Quantitative chemical proteomics reveals mechanisms of action of clinical ABL kinase inhibitors
    Marcus Bantscheff
    Cellzome AG, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 25:1035-44. 2007
    ..The data suggest that our approach is a valuable tool for drug discovery...
  4. pmc Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1
    G Asher
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 98:1188-93. 2001
    ..Dicoumarol also reduced the level of p53 in its mutant form in M1 cells. The results indicate that NQO1 plays an important role in regulating p53 functions by inhibiting its degradation...
  5. ncbi Two minor NQO1 and NQO2 alleles predict poor response of breast cancer patients to adjuvant doxorubicin and cyclophosphamide therapy
    David Jamieson
    Northern Institute for Cancer Research, Paul O Gorman Building, Medical School, Newcastle University, Newcastle upon Tyne, UK
    Pharmacogenet Genomics 21:808-19. 2011
    ..We aimed to determine whether functional NQO2 variants are associated with altered response to adjuvant doxorubicin and cyclophosphamide therapy, with or without tamoxifen, in the treatment of breast cancer...
  6. ncbi New roles of flavoproteins in molecular cell biology: an unexpected role for quinone reductases as regulators of proteasomal degradation
    Sonja Sollner
    Technische Universitat Graz, Institut fur Biochemie, Austria
    FEBS J 276:4313-24. 2009
    b>Quinone reductases are ubiquitous soluble enzymes found in bacteria, fungi, plants and animals...
  7. ncbi Superoxide dismutase and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase polymorphisms and pancreatic cancer risk
    Beatrice Mohelnikova-Duchonova
    Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic
    Pancreas 40:72-8. 2011
    ....
  8. pmc Crystal structure of quinone reductase 2 in complex with resveratrol
    Leonid Buryanovskyy
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Biochemistry 43:11417-26. 2004
    ....
  9. ncbi Association of superoxide dismutases and NAD(P)H quinone oxidoreductases with prognosis of patients with breast carcinomas
    Miluse Hubackova
    Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic
    Int J Cancer 130:338-48. 2012
    ..004). Our results suggest that NQO2, SOD2 and SOD3 may significantly modify prognosis of breast cancer patients and that their significance should be further characterized...
  10. ncbi Insights into the redox cycle of human quinone reductase 2
    Karine Reybier
    Université de Toulouse 3, UPS, UMR 152 PHARMA DEV, 118 route de Narbonne, F 31062 Toulouse Cedex 9, France
    Free Radic Res 45:1184-95. 2011
    ..In conclusion, EPR analysis of QR2 enzyme activity through free radical production enables modulators and effective inhibitors to be distinguished...
  11. pmc New insights into type II NAD(P)H:quinone oxidoreductases
    Ana M P Melo
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica, Apartado 127, 2781 901 Oeiras, Portugal
    Microbiol Mol Biol Rev 68:603-16. 2004
    ..The latter showed that most organisms contain genes that potentially encode NDH-2. An overview of this development is presented, with special emphasis on microbial enzymes and on the identification of three subfamilies of NDH-2...
  12. pmc Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3
    Jun Jie Zhang
    Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
    J Bacteriol 191:2703-10. 2009
    ..J. Moonen, N. M. Kamerbeek, A. H. Westphal, S. A. Boeren, D. B. Janssen, M. W. Fraaije, and W. J. van Berkel, J. Bacteriol. 190:5190-5198, 2008), suggesting that the genes involved in PNP degradation are physically linked...
  13. ncbi NRH:quinone oxidoreductase 2 and NAD(P)H:quinone oxidoreductase 1 protect tumor suppressor p53 against 20s proteasomal degradation leading to stabilization and activation of p53
    Xing Gong
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    Cancer Res 67:5380-8. 2007
    ..These results suggest that stress-induced NQO1 and NQO2 transiently stabilize p53, which leads to protection against adverse effects of stressors...
  14. ncbi NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain
    K Matsushita
    Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Biochemistry 26:7732-7. 1987
    ....
  15. ncbi Flavin-dependent quinone reductases
    S Deller
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 II, A 8010, Graz, Austria
    Cell Mol Life Sci 65:141-60. 2008
    ..This family of flavin-dependent quinone reductases shares a flavodoxin-like structure and reaction mechanism pointing towards a common evolutionary origin...
  16. ncbi Steady-state kinetics and inhibitory action of antitubercular phenothiazines on mycobacterium tuberculosis type-II NADH-menaquinone oxidoreductase (NDH-2)
    Takahiro Yano
    Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:11456-63. 2006
    ..Trifluoperazine inhibition is non-competitive for NADH, whereas the inhibition kinetics is found to be uncompetitive in terms of Q2...
  17. pmc Inhibitors of type II NADH:menaquinone oxidoreductase represent a class of antitubercular drugs
    Edward A Weinstein
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:4548-53. 2005
    ..Several phenothiazines analogs are highly tuberculocidal in vitro, suppress Mtb growth in a mouse model of acute infection, and represent lead compounds that may give rise to a class of selective antibiotics...
  18. ncbi Kinetic mechanism of quinone oxidoreductase 2 and its inhibition by the antimalarial quinolines
    Jesse J Kwiek
    Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710, USA
    Biochemistry 43:4538-47. 2004
    ..Our studies shed light on the possible in vivo potency of the quinolines and provide a foundation for future studies aimed at creating more potent QR2 inhibitors and at understanding the physiological significance of QR2...
  19. ncbi Type II NADH: menaquinone oxidoreductase of Mycobacterium tuberculosis
    J S Teh
    Infectious Diseases, School of Medicine, University of Pennsylvania, USA
    Infect Disord Drug Targets 7:169-81. 2007
    ..In this chapter, we critically review the recent advances in this field, with particular emphasis on NDH-2, and underscore the kinds of research further needed for drug development...
  20. ncbi Flavin adenine dinucleotide content of quinone reductase 2: analysis and optimization for structure-function studies
    Kevin Ka Ki Leung
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Anal Biochem 420:84-9. 2012
    ..This method of purification and reconstitution will help to optimize structural and functional studies of NQO2 and possibly other flavoproteins...
  21. ncbi A dual role for plant quinone reductases in host-fungus interaction
    Eiri Heyno
    Department of Plant Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel
    Physiol Plant 149:340-53. 2013
    b>Quinone reductases (QR, EC 1.5.6.2) are flavoproteins that protect organisms from oxidative stress. The function of plant QRs has not as yet been addressed in vivo despite biochemical evidence for their involvement in redox reactions...
  22. ncbi Mechanism of sulfide-quinone reductase investigated using site-directed mutagenesis and sulfur analysis
    Christoph Griesbeck
    Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universitat Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    Biochemistry 41:11552-65. 2002
    ..On the basis of these data, reaction mechanisms for sulfide-dependent reduction and quinone-dependent oxidation of the enzyme and for the formation of polysulfide are proposed...
  23. pmc Identification, design and biological evaluation of heterocyclic quinolones targeting Plasmodium falciparum type II NADH:quinone oxidoreductase (PfNDH2)
    Suet C Leung
    Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK
    J Med Chem 55:1844-57. 2012
    ..falciparum providing the potential added benefit of a dual mechanism of action. The potent oral activity of 2-pyridyl quinolones underlines the potential of this template for further lead optimization studies...
  24. ncbi Structure of Escherichia coli YhdH, a putative quinone oxidoreductase
    Gerlind Sulzenbacher
    AFMB, UMR 6098, CNRS and Universités Aix Marseille I and II, 31 Chemin J Aiguier, F 13402 Marseille 20, France
    Acta Crystallogr D Biol Crystallogr 60:1855-62. 2004
    ..6 A resolution, respectively. The overall fold of YhdH is very similar to that of alcohol dehydrogenases and quinone reductases despite its low sequence identity...
  25. ncbi Chemical proteomic profiles of the BCR-ABL inhibitors imatinib, nilotinib, and dasatinib reveal novel kinase and nonkinase targets
    Uwe Rix
    Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna
    Blood 110:4055-63. 2007
    ..4) The oxidoreductase NQO2 was bound and inhibited by imatinib and nilotinib at physiologically relevant drug concentrations, representing the first nonkinase target of these drugs...
  26. ncbi Quinone reductases multitasking in the metabolic world
    David Ross
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Drug Metab Rev 36:639-54. 2004
    ....
  27. ncbi Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
    ..With an E. coli mutant deficient in complex I, the Na+ transport activity was low (1-3 nmol mg-1 min-1), and rotenone was without effect...
  28. ncbi Single eubacterial origin of eukaryotic sulfide:quinone oxidoreductase, a mitochondrial enzyme conserved from the early evolution of eukaryotes during anoxic and sulfidic times
    Ursula Theissen
    Institute of Botany III, University of Dusseldorf, Dusseldorf, Germany
    Mol Biol Evol 20:1564-74. 2003
    ....
  29. ncbi Chapter 17 Type II NADH: quinone oxidoreductases of Plasmodium falciparum and Mycobacterium tuberculosis kinetic and high-throughput assays
    Nicholas Fisher
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom
    Methods Enzymol 456:303-20. 2009
    ..In addition, owing to the interest in ndhs as potential chemotherapeutic targets, we describe a miniaturized endpoint assay that is validated for high-throughput screening (HTS) of chemical libraries...
  30. ncbi NRH:quinone reductase 2: an enzyme of surprises and mysteries
    Fanny Vella
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 125, chemin de Ronde 78290 Croissy sur Seine, France
    Biochem Pharmacol 71:1-12. 2005
    ..Finally QR2 might be implicated in the toxicity, in vivo, of quinones such as menadione. The present commentary attempts to summarize this information and discusses a series of hypotheses...
  31. ncbi Characterization of the melatoninergic MT3 binding site on the NRH:quinone oxidoreductase 2 enzyme
    François Mailliet
    Division de Pharmacologie Moleculaire et Cellulaire, Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy sur Seine, France
    Biochem Pharmacol 71:74-88. 2005
    ..The compounds described in the present paper are new tools for such a task...
  32. pmc Were there any "misassignments" among iron-sulfur clusters N4, N5 and N6b in NADH-quinone oxidoreductase (complex I)?
    Tomoko Ohnishi
    Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
    Biochim Biophys Acta 1777:703-10. 2008
    ..As to the claim of "misassignment" between clusters N4 and N6b, that was not a possibility because our mutagenesis systems did not contain cluster N6b. Therefore, we believe that we have not made any "misassignment" in our work...
  33. pmc The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration
    Marco Marcia
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 106:9625-30. 2009
    ....
  34. ncbi Mitochondrial quinone reductases: complex I
    Giorgio Lenaz
    Dipartimento di Biochemica, Universita di Bologna, Italy
    Methods Enzymol 382:3-20. 2004
  35. ncbi Deficiency of NRH:quinone oxidoreductase 2 increases susceptibility to 7,12-dimethylbenz(a)anthracene and benzo(a)pyrene-induced skin carcinogenesis
    Karim Iskander
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    Cancer Res 64:5925-8. 2004
    ..The results also suggest that lack of induction of p53, p21, and Bax proteins might contribute to increased sensitivity of NQO2-/- mice skin to benzo(a)pyrene carcinogenicity...
  36. pmc Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2
    Barbara Calamini
    Center for Pharmaceutical Biotechnology and Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The University of Illinois at Chicago, 900 S Ashland Ave M C 870, Chicago, IL 60607, USA
    Biochem J 413:81-91. 2008
    ..These results provide new insights into the binding mechanisms of melatonin and analogues to QR2...
  37. ncbi Disruption of dihydronicotinamide riboside:quinone oxidoreductase 2 (NQO2) leads to myeloid hyperplasia of bone marrow and decreased sensitivity to menadione toxicity
    Delwin J Long
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 277:46131-9. 2002
    ..The NQO2-null mice are a model for NQO2 deficiency in humans and can be used to determine the role of this enzyme in sensitivities to toxicity and carcinogenesis...
  38. ncbi Binding of the anticancer prodrug CB1954 to the activating enzyme NQO2 revealed by the crystal structure of their complex
    Majed AbuKhader
    School of Pharmacy and The Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Med Chem 48:7714-9. 2005
    ..The structure also reveals an unfavorable interaction, therefore suggesting possible avenues for DEPT-tailored engineering studies...
  39. ncbi Crystal structure of quinone reductase 2 in complex with cancer prodrug CB1954
    Yue Fu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Biochem Biophys Res Commun 336:332-8. 2005
    CB1954 is a cancer pro-drug that can be activated through reduction by Escherichia coli nitro-reductases and quinone reductases. Human quinone reductase 2 is very efficient in the activation of CB1954, approximately 3000 times more ..
  40. pmc Differential stress-induced regulation of two quinone reductases in the brown rot basidiomycete Gloeophyllum trabeum
    Roni Cohen
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 70:324-31. 2004
    b>Quinone reductases (QRDs) have two important functions in the basidiomycete Gloeophyllum trabeum, which causes brown rot of wood. First, a QRD is required to generate biodegradative hydroxyl radicals via redox cycling between two G...
  41. ncbi NAD(P)H:quinone oxidoreductase 1 and nrh:quinone oxidoreductase 2 activity and expression in bladder and ovarian cancer and lower NRH:quinone oxidoreductase 2 activity associated with an NQO2 exon 3 single-nucleotide polymorphism
    David Jamieson
    Northern Institute for Cancer Research, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Clin Cancer Res 13:1584-90. 2007
    ..The NQO activity of ovarian and bladder tumors was determined and the effect of NQO polymorphisms on NQO activity was investigated...
  42. pmc Inhibition of membrane-bound methane monooxygenase and ammonia monooxygenase by diphenyliodonium: implications for electron transfer
    Andrew K Shiemke
    Department of Biochemistry and Molecular Pharmacology, Robert C Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, West Virginia 26506 9142, USA
    J Bacteriol 186:928-37. 2004
    ..DPI does not affect hydroxylamine oxidoreductase activity and does not require AMO turnover to exert its inhibitory effect. Implications of these data for the electron transfer pathway from the quinone pool to pMMO and AMO are discussed...
  43. pmc Identification, design and biological evaluation of bisaryl quinolones targeting Plasmodium falciparum type II NADH:quinone oxidoreductase (PfNDH2)
    Chandrakala Pidathala
    Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK
    J Med Chem 55:1831-43. 2012
    ..Other quinolones presented (e.g., 6d, 6f, 14e) have the capacity to inhibit both PfNDH2 and P. falciparum cytochrome bc(1), and studies to determine the potential advantage of this dual-targeting effect are in progress...
  44. ncbi Characterization of the iron-sulfur cluster N7 (N1c) in the subunit NuoG of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli
    Eiko Nakamaru-Ogiso
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:301-7. 2005
    ....
  45. ncbi An unexpected effect of 5-MCA-NAT in chick retinal development
    Lucia de Fatima Sobral Sampaio
    Laboratório de Bioquímica do Desenvolvimento do Sistema Nervoso, Instituto de Ciencias Biologicas, Universidade Federal do Para, Rua Augusto Correa, 1, CEP 66075 110 Belém, PA, Brazil
    Int J Dev Neurosci 27:511-5. 2009
    ....
  46. ncbi NRH:quinone oxidoreductase 2 (NQO2) catalyzes metabolic activation of quinones and anti-tumor drugs
    Claudia M Celli
    Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Biochem Pharmacol 72:366-76. 2006
    ..These results concluded that NQO2 plays a significant role in the metabolic activation of both quinones and anti-tumor drugs leading to cytotoxicity and cell death...
  47. ncbi Differential regulation of wheat quinone reductases in response to powdery mildew infection
    David L Greenshields
    Department of Biology, University of Saskatchewan, Saskatoon, SK, S7N 5E2, Canada
    Planta 222:867-75. 2005
    At least two types of quinone reductases are present in plants: (1) the zeta-crystallin-like quinone reductases (QR1, EC 1.6.5...
  48. pmc A unique cytosolic activity related but distinct from NQO1 catalyses metabolic activation of mitomycin C
    P Joseph
    Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
    Br J Cancer 82:1305-11. 2000
    ..This activity, like NQO1, was inhibited by dicoumarol and immunologically related to NQO1...
  49. ncbi Two polycyclic aromatic hydrocarbon o-quinone reductases from a pyrene-degrading Mycobacterium
    Yong Hak Kim
    National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Arch Biochem Biophys 416:209-17. 2003
    ..80) and PQ (5.19) than PQR2 (13.9 for menadione and 176 for PQ). Additionally, PQR2 exhibited a broad substrate specificity with high specificity constants for 1,4-naphthalenequinone, 1,2-naphthalenequinone, and PyQ...
  50. ncbi Thermodynamic properties of the redox centers of Na(+)-translocating NADH:quinone oxidoreductase
    Alexander V Bogachev
    Department of Molecular Energetics of Microorganisms, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119899, Russia
    Biochemistry 45:3421-8. 2006
    ..On the basis of these data, plausible mechanisms for the translocation of transmembrane sodium ions by Na(+)-NQR are discussed...
  51. ncbi Inhibitor studies on non-photochemical plastoquinone reduction and H(2) photoproduction in Chlamydomonas reinhardtii
    Florence Mus
    CEA Cadarache, DSV DEVM Laboratoire d Ecophysiologie de la Photosynthèse, UMR 6191 CNRS CEA, Aix Marseille II, F 13108 Saint Paul lez Durance Cedex, France
    Biochim Biophys Acta 1708:322-32. 2005
    ..These results are discussed in relation to the absence of ndh genes in Chlamydomonas plastid genome and to the existence of 7 ORFs homologous to type-II NDHs in its nuclear genome...
  52. ncbi Nitrate reduction by Desulfovibrio desulfuricans: a periplasmic nitrate reductase system that lacks NapB, but includes a unique tetraheme c-type cytochrome, NapM
    Angeliki Marietou
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    FEMS Microbiol Lett 248:217-25. 2005
    ..We also suggest that, despite the absence of a twin-arginine targeting sequence, NapG might be located in the periplasm where it would provide an alternative direct electron donor to NapA...
  53. pmc Membrane topology mapping of the Na+-pumping NADH: quinone oxidoreductase from Vibrio cholerae by PhoA-green fluorescent protein fusion analysis
    Ellen B Duffy
    Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, NY 12180, USA
    J Bacteriol 188:8343-51. 2006
    ..The finding that all the redox cofactors are localized to the cytoplasmic side of the membrane is discussed...
  54. ncbi X- and W-band EPR and Q-band ENDOR studies of the flavin radical in the Na+ -translocating NADH:quinone oxidoreductase from Vibrio cholerae
    Blanca Barquera
    Department of Biochemistry University of Illinois at Urbana Champaign, 600 South Mathews Street, Urbana, IL 61801, USA
    J Am Chem Soc 125:265-75. 2003
    ..Alternatively, both forms of the radical signal may arise from a single, extremely stable, flavin semiquinone, which becomes deprotonated upon reduction of the enzyme...
  55. ncbi Photosynthetic activity in winter needles of the evergreen tree Taxus cuspidata at low temperatures
    Ayumi Tanaka
    Institute of Low Temperature Science, Hokkaido University, W8 N10, Sapporo 060 0819, Japan
    Tree Physiol 27:641-8. 2007
    ..The majority of Q(A) remained reduced even when winter needles were subjected to a temperature of -5 degrees C at low irradiance...
  56. ncbi A vertebrate-type ferredoxin domain in the Na+-translocating NADH dehydrogenase from Vibrio cholerae
    Po Chi Lin
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Honggerberg, Zurich, Switzerland
    J Biol Chem 280:22560-3. 2005
    ..This study reveals a novel function for vertebrate-type [2Fe-2S] clusters as redox cofactors in respiratory dehydrogenases...
  57. pmc Crystallization of the NADH-oxidizing domain of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae
    Minli Tao
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:110-2. 2006
    ..Crystals diffract to 2.8 A and belong to space group P622, with unit-cell parameters a = b = 145.3, c = 90.2 A, alpha = beta = 90, gamma = 120 degrees...
  58. ncbi The origin of the sodium-dependent NADH oxidation by the respiratory chain of Klebsiella pneumoniae
    Yulia V Bertsova
    Department of Molecular Energetics of Microorganisms, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119899, Russia
    FEBS Lett 563:207-12. 2004
    ..It is concluded that the NQR-type enzyme, not the NDH-1-type enzyme, catalyzes sodium-dependent NADH oxidation in K. pneumoniae...
  59. ncbi Structure-activity relationships in two-electron reduction of quinones
    Narimantas Cenas
    Institute of Biochemistry, Vilnius, Lithuania
    Methods Enzymol 382:258-77. 2004
  60. ncbi Involvement of sulfide:quinone oxidoreductase in sulfur oxidation of an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans NASF-1
    Satoshi Wakai
    Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Biosci Biotechnol Biochem 68:2519-28. 2004
    ..A model is proposed for the oxidation of reduced inorganic sulfur compounds in A. ferrooxidans NASF-1 cells...
  61. ncbi Characterization of a HMT2-like enzyme for sulfide oxidation from Pseudomonas putida
    Hiroomi Shibata
    School of Agriculture, Meiji University, Higahimita 1 1 1, Kawasaki, 214 8571, Japan
    Can J Microbiol 52:724-30. 2006
    ..The sulfide-quinone oxidoreductase activity in P. putida KT2440 was attributable to the presence of pp0053, and the activity showed a close relevance to enzymatic activities related to sulfur assimilation...
  62. pmc Covalent binding of flavins to RnfG and RnfD in the Rnf complex from Vibrio cholerae
    Julianne Backiel
    Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 Eight Street, Troy, New York 12180, USA
    Biochemistry 47:11273-84. 2008
    ..In contrast, in NqrC and NqrB the flavins are located in a cytoplasmic loop. This topological analysis suggests that there may be mechanistic differences between the Rnf and Na+-NQR complexes...
  63. ncbi Quinone oxidoreductases of the plasma membrane
    D James Morre
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, Lafayette, Indiana 47907, USA
    Methods Enzymol 378:179-99. 2004
  64. pmc Reduction of mitomycin C is catalysed by human recombinant NRH:quinone oxidoreductase 2 using reduced nicotinamide adenine dinucleotide as an electron donating co-factor
    D Jamieson
    Northern Institute for Cancer Research, University of Newcastle upon Tyne, Paul O Gorman Building, Medical School, Newcastle upon Tyne NE2 4HH, UK
    Br J Cancer 95:1229-33. 2006
    ..These data indicate that NQO2 may contribute to the metabolism of MMC to cytotoxic species...
  65. ncbi NADH oxidation by the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae: functional role of the NqrF subunit
    Karin Türk
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    J Biol Chem 279:21349-55. 2004
    ..The observed electron transfer NADH --> FAD --> [2Fe-2S] in NqrF requires positioning of the FAD and the Fe-S cluster in close proximity in accordance with a structural model of the subunit...
  66. pmc Regulatory loop between redox sensing of the NADH/NAD(+) ratio by Rex (YdiH) and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis
    Smita Gyan
    International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183 8509, Japan
    J Bacteriol 188:7062-71. 2006
    ..These results indicated that Rex and Ndh together form a regulatory loop which functions to prevent a large fluctuation in the NADH/NAD(+) ratio in B. subtilis...
  67. ncbi Quinol:fumarate oxidoreductases and succinate:quinone oxidoreductases: phylogenetic relationships, metal centres and membrane attachment
    Rita S Lemos
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Biochim Biophys Acta 1553:158-70. 2002
    ..Finally, the possible contribution of these enzymes to the formation/dissipation of a transmembrane proton gradient is discussed, considering recent experimental and structural data...
  68. ncbi Recent progress in the Na(+)-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    Biochim Biophys Acta 1505:37-44. 2001
    ..A novel antibiotic, korormicin, was found to specifically inhibit the NQR complex. From the homology search of the nqr operon, it was found that the Na(+)-pumping NQR complex is widely distributed among Gram-negative pathogenic bacteria...
  69. ncbi Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 356:330-2. 1994
    ..Among the subclones selected by probe C, the expression of the beta-subunit as a gene product was detected in Escherichia coli membranes by activity staining and Western blotting...
  70. ncbi Increased level of the mitochondrial ND5 transcript in chemically induced rat hepatomas
    M Corral
    Institut de pathologie moleculaire, U S A CNRS 1147, Paris, France
    Exp Cell Res 184:158-66. 1989
    ..Southern blot analysis showed a mitochondrial DNA heterogeneity in hepatomas with an alteration of the structure of part of the molecules...
  71. ncbi FMN is covalently attached to a threonine residue in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba, Japan
    FEBS Lett 488:5-8. 2001
    ..The phosphoester binding of FMN to a threonine residue reported here is a new type of flavin attachment to a polypeptide...
  72. ncbi Covalently bound flavin in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    FEBS Lett 474:165-8. 2000
    ..This is the first example that the flavin is linked to a threonine residue. The amino acid sequence around the flavin-linked threonine was well conserved between NqrB and NqrC. Identification of the flavin group is in progress...
  73. ncbi Detection of the Na(+)-translocating NADH-quinone reductase in marine bacteria using a PCR technique
    S Kato
    New Energy and Industrial Technology Development Organization NEDO, Marine Biotechnology Institute Co, Ltd, Tokyo, Japan
    Can J Microbiol 46:325-32. 2000
    ..The nucleotide and predicted amino acid sequences of nqr6 were highly conserved among the PCR-positive marine bacteria. A phylogenetic analysis of marine bacteria, based on nqr6 sequencing, was performed...
  74. ncbi Crystal structure of a new type of NADPH-dependent quinone oxidoreductase (QOR2) from Escherichia coli
    In Kwon Kim
    Laboratory of Biophysics, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151 742, Republic of Korea
    J Mol Biol 379:372-84. 2008
    ....
  75. ncbi The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme
    L M Garcia
    Departamento de Bioquimica, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico, DF Mexico
    Can J Microbiol 54:456-66. 2008
    ..The physiological properties and genome database analyses of B. cereus are consistent with the cereus group ancestor being an opportunistic pathogen...
  76. ncbi The Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus--redox states of the FAD prosthetic group and mechanism of Ag+ inhibition
    J Steuber
    ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 249:770-6. 1997
    ..The increase in fluorescence intensity correlated with the loss of enzyme activity. Gel filtration of the Ag+-treated Na+-NQR confirmed that FAD had been displaced from the holo-enzyme...
  77. ncbi Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 363:75-7. 1995
    ..FEBS Lett. 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex...
  78. ncbi Na(+)-Translocating NADH:quinone oxidoreductase: progress achieved and prospects of investigations
    A V Bogachev
    Department of Molecular Energetics of Microorganisms, Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia
    Biochemistry (Mosc) 70:143-9. 2005
    ..Based on the existing data, possible model mechanisms of sodium transfer by Na+-translocating NADH:quinone oxidoreductase are proposed...
  79. ncbi Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus
    P Beattie
    Institute for Cell and Molecular Biology, Edinburgh University, UK
    FEBS Lett 356:333-8. 1994
    ..From sequence comparisons, we conclude that the Na(+)-dependent NADH-ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H(+)-dependent enzymes of both prokaryotes and eukaryotes...
  80. ncbi Na(+) translocation by bacterial NADH:quinone oxidoreductases: an extension to the complex-I family of primary redox pumps
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1505:45-56. 2001
    ..alginolyticus, higher (Na(+) or H(+)) transport stoichiometries are expected for complex I, suggesting the presence of a second coupling site...
  81. ncbi Expression and mutagenesis of the NqrC subunit of the NQR respiratory Na(+) pump from Vibrio cholerae with covalently attached FMN
    B Barquera
    Department of Biochemistry, University of Illinois at Urbana Champaign, Urbana, 61801, USA
    FEBS Lett 492:45-9. 2001
    ..In contrast, no covalent flavin was detected when threonine-225 was replaced by leucine. The data show that the FMN attachment does not require assembly of the enzyme and are consistent with the unusual threonine attachment site...
  82. pmc FQR1, a novel primary auxin-response gene, encodes a flavin mononucleotide-binding quinone reductase
    Marta J Laskowski
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    Plant Physiol 128:578-90. 2002
    ..Sequence analysis reveals that FQR1 belongs to a family of flavin mononucleotide-binding quinone reductases. Partially purified His-tagged FQR1 isolated from Escherichia coli catalyzes the transfer of electrons from ..
  83. ncbi Mutations in cytochrome b that affect kinetics of the electron transfer reactions at center N in the yeast cytochrome bc1 complex
    Frederik A J Rotsaert
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail, Hanover, New Hampshire 03755, USA
    Biochim Biophys Acta 1777:239-49. 2008
    ..These results show that one can distinguish between the contribution of structural and thermodynamic factors to center N function...
  84. ncbi Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species
    Julia Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 269:1287-92. 2002
    ..alginolyticus. It is shown that the [2Fe-2S] cluster previously assigned to the Na+-NQR originates from the succinate dehydrogenase or the related enzyme fumarate reductase...
  85. ncbi Characterization of the membrane domain Nqo11 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 41:4377-84. 2002
    ..coli membranes have been performed by using cysteine mapping and immunochemical analyses. The data suggest that the Nqo11 subunit has three transmembrane segments and its C-terminus protrudes into the cytoplasmic phase...
  86. ncbi Characterization of the iron-sulfur cluster coordinated by a cysteine cluster motif (CXXCXXXCX27C) in the Nqo3 subunit in the proton-translocating NADH-quinone oxidoreductase (NDH-1) of Thermus thermophilus HB-8
    Eiko Nakamaru-Ogiso
    Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:1680-8. 2002
    ..Considering the fact that the same motif coordinates only tetranuclear clusters in other enzymes so far known, we propose that the CXXCXXXCX(27)C motif in the Nqo3 subunit most likely ligates the [4Fe-4S] cluster...
  87. ncbi Redox-dependent sodium binding by the Na(+)-translocating NADH:quinone oxidoreductase from Vibrio harveyi
    Alexander V Bogachev
    Department of Molecular Energetics of Microorganisms, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119899, Russia
    Biochemistry 46:10186-91. 2007
    ..The data indicate that energy conservation by NQR involves a mechanism modulating ion affinity by the redox state of an enzyme redox cofactor...
  88. ncbi Korormicin insensitivity in Vibrio alginolyticus is correlated with a single point mutation of Gly-140 in the NqrB subunit of the Na(+)-translocating NADH-quinone reductase
    Maki Hayashi
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Arch Biochem Biophys 401:173-7. 2002
    ..The fact that korormicin is a purely noncompetitive inhibitor for Q-1 strongly supports the presence of one of Q-1 binding sites in the NqrB subunit, which also has a covalently bound FMN at Thr-235...
  89. ncbi Rat liver NAD(P)H: Quinone reductase. Regulation of quinone reductase gene expression by planar aromatic compounds and determination of the exon structure of the quinone reductase structural gene
    R M Bayney
    Department of Molecular Pharmacology and Biochemistry, Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
    J Biol Chem 264:21793-7. 1989
    ..These data suggest the presence of a cis-acting regulatory element(s) in the 5'-flanking region of the quinone reductase structural gene which regulates inducible expression...
  90. ncbi The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus
    L R Swem
    Department of Biology, Indiana University, Bloomington 47405, USA
    J Mol Biol 309:121-38. 2001
    ..These data provide evidence that the RegB/RegA two-component system has a major role in controlling the synthesis of numerous processes that affect reducing equivalents in Rhodobacter capsulatus...
  91. ncbi Site-directed mutation of the highly conserved region near the Q-loop of the cytochrome bd quinol oxidase from Escherichia coli specifically perturbs heme b595
    J Zhang
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 40:8548-56. 2001
    ..This is the first report of a mutation that specifically affects the binding site of heme b(595)...
  92. ncbi Structural and sequence comparisons of quinone oxidoreductase, zeta-crystallin, and glucose and alcohol dehydrogenases
    K J Edwards
    Centre for Molecular Structure and Function, Research School of Chemistry, Australian National University, Canberra, Australia
    Arch Biochem Biophys 328:173-83. 1996
    ..Residues which are important for catalysis have been altered and the functions and activities of the enzymes have diverged, illustrating a classic example of divergent evolution among a superfamily of enzymes...
  93. ncbi Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 422:240-2. 1998
    ..By modifying the detection method of proteins on SDS-PAGE, we could detect all six subunits encoded by NQR operon in the purified NQR complex. The open reading frame of each subunit was identified from its N-terminal amino acid sequence...
  94. ncbi Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:9545-54. 2005
    ..Possible roles of these arginine residues and other conserved residues in the NuoK subunit for NDH-1 function were discussed...
  95. ncbi Transmembrane orientation and topology of the NADH:quinone oxidoreductase putative quinone binding subunit NuoH
    R Roth
    Department of Biochemistry, Lund University, Box 124, 22100, Lund, Sweden
    Biochim Biophys Acta 1504:352-62. 2001
    ..The N-terminal and C-terminal ends are located on the outside of the membrane. A topology model of NuoH based on these results is presented, and implications from the model are discussed...
  96. ncbi Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase
    S J Pilkington
    Medical Research Council Laboratory of Molecular Biology, Cambridge, U K
    Biochemistry 30:2166-75. 1991
    ....
  97. ncbi Crystal structure of a putative NADPH-dependent oxidoreductase (GI: 18204011) from mouse at 2.10 A resolution
    Inna Levin
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 56:629-33. 2004
  98. ncbi The nitroreductase enzyme in Walker cells that activates 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) to 5-(aziridin-1-yl)-4-hydroxylamino-2-nitrobenzamide is a form of NAD(P)H dehydrogenase (quinone) (EC 1.6.99.2)
    R J Knox
    Molecular Pharmacology Unit, Institute of Cancer Research, Sutton, Surrey, U K
    Biochem Pharmacol 37:4671-7. 1988
    ..This observation could explain the reported antagonistic action of the aminoimidazole carboxamides to the antitumour effects of CB 1954...
  99. ncbi Regulation of expression of Na+ -translocating NADH:quinone oxidoreductase genes in Vibrio harveyi and Klebsiella pneumoniae
    Maria S Fadeeva
    Department of Molecular Energetics of Microorganisms, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Leninskie Gory, Moscow 119992, Russia
    Arch Microbiol 188:341-8. 2007
    ..harveyi during anaerobic growth, and nqr expression is modulated by electron acceptors and values of their redox potentials. The latter effect was shown to be independent of the ArcAB regulatory system...
  100. ncbi Induction of NAD(P)H quinone oxidoreductase and glutathione S-transferase activities in livers of female August-Copenhagen Irish rats treated chronically with estradiol: comparison with the Sprague-Dawley rat
    Rosa I Sanchez
    Laboratory for Cellular and Biochemical Toxicology, College of Pharmacy, Rutgers, The State University of New Jersey, 41 Gordon Road, Piscataway, NJ 08854, USA
    J Steroid Biochem Mol Biol 87:199-206. 2003
    ....
  101. pmc MAK-4 and -5 supplemented diet inhibits liver carcinogenesis in mice
    Marialetizia Penza
    Laboratory of Biotechnology, Civic Hospital of Brescia, Brescia, Italy
    BMC Complement Altern Med 7:19. 2007
    ..MAK-4 and MAK-5 are each composed of different herbs and are said to have maximum benefit when used in combination. This investigation evaluated the cancer inhibiting effects of MAK-4 and MAK-5, in vitro and in vivo...

Research Grants23

  1. Novel Mechanisms of Quinone Toxicity
    David Ross; Fiscal Year: 2013
    ..The major mammalian quinone reductases NQO1 and NQO2 are highly polymorphic with a high prevalence of variant alleles resulting in marked ..
  2. Aldo-Keto Reductases and PAH Metabolism/Activation
    Trevor M Penning; Fiscal Year: 2013
    ..Four aims will elaborate the role of the AKR pathway in cancer of the airway. In Aim#1, the phase I [quinone reductases (QR)] enzymes that redox-cycle the PAH o-quinones will be identified...
  3. Signal transduction in cytoprotective responses
    Mihail Iordanov; Fiscal Year: 2006
    ..enzymes (a group of conjugating enzymes that includes, among others, glutathione S-transferases and quinone reductases) are the major cellular defense system against toxic environmental and metabolic electrophiles...
  4. Chemoprevention of prostate cancer by resveratrol
    Tze Chen Hsieh; Fiscal Year: 2005
    ....
  5. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
  6. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2008
    ..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
  7. NQO1 Inhibitors and Pancreatic Cancer Therapy
    David Ross; Fiscal Year: 2008
    ..This approach represents a novel therapeutic strategy for the treatment of pancreatic cancer, a disease where therapeutic options are very limited and where chemotherapy has made minimal impact. ..
  8. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2009
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  9. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond F Burk; Fiscal Year: 2010
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  10. Na+-pumping NADH:quinone oxidoreductase of V. cholerae
    Blanca Barquera; Fiscal Year: 2009
    ..We will also make a strong effort to crystallize Na+-NQR, since a 3-dimensional structural model is essential for a molecular-level understanding of the mechanism of the enzyme. ..
  11. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ....
  12. NQO1 in Protection Against Benzene Toxicicity
    David Ross; Fiscal Year: 2007
    ..abstract_text> ..
  13. NQ01, Oxidative Stress and Proteasomal Inhibition
    David Ross; Fiscal Year: 2006
    ..The ability of mutant NQO1 *2 protein to generate oxygen radicals becomes particularly important under conditions where the UPS is inhibited and the protein accumulates. ..
  14. Ferritin and Iron Nutrition in Health and Disease
    Elizabeth Theil; Fiscal Year: 2005
    ..The results will clarify mechanisms of ferritin iron uptake and characterize molecular genetic differences in iron uptake for improving dietary iron sources in health and disease. ..
  15. Chemopreventive Efficacy of Broccoli Sprouts in Humans
    Thomas Kensler; Fiscal Year: 2004
    ..abstract_text> ..
  16. Workshop on BioIron in Thalassemia, Sickle Cell Disease and Hemochromatosis
    Elizabeth Theil; Fiscal Year: 2004
    ..Fuller understanding of iron homeostasis in Thalassemia, Sickle Cell Disease and Hemochromatosis and improved disease management strategies can be expected. ..
  17. Workshop on Biolron in Thalassemia & Sickle Cell Disease
    Elizabeth Theil; Fiscal Year: 2002
    ..abstract_text> ..
  18. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2003
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  19. IRON & OXYGEN BIOLOGY IN SCD, HH & BETA-THAL MICE
    Elizabeth Theil; Fiscal Year: 2002
    ..The resulting information will be used to help probe the efficiency of presently available and experimental chelators, not only in promoting iron excretion but also in the prevention of defined types of cellular damage. ..
  20. Protection by Induction of Ubiquitin-Proteasome Systems
    Thomas Kensler; Fiscal Year: 2005
    ....
  21. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2011
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  22. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2003
    ..2) Effects of MPTP on transgenic mice expressing the Ndil enzyme in the dopamine-producing tissues. ..
  23. GENETIC MODELS TO STUDY ALCOHOL TOXICITY
    Vasilis Vasiliou; Fiscal Year: 2003
    ..These studies will greatly enhance our understanding of genetic factors involved in alcohol toxicity. ..