Genomes and Genes
nadph oxidoreductases nadh
Summary: A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6.
Publications214 found, 100 shown here
- Redox-dependent change of nucleotide affinity to the active site of the mammalian complex IVera G Grivennikova
Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
Biochemistry 46:10971-8. 2007..Possible models to explain the different titration patterns for the forward and reverse reactions are briefly discussed...
- NAD(P)H oxidase activity in cultured human podocytes: effects of adenosine triphosphateS Greiber
Abteilung Neprologie, Universitätsklink Freiburg, Germany
Kidney Int 53:654-63. 1998..Activation of NAD(P)H oxidase by ATP might be secondary to increased mRNA expression of the NADPH oxidase subunit gp67phox...
- The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coatNathalie Nesi
UMR118 INRA Agrocampus Ouest Université de Rennes1 Amélioration des Plantes et Biotechnologies Végétales, Le Rheu Cedex, France
Plant Cell Rep 28:601-17. 2009....
- Recent developments in pyridine nucleotide regenerationWilfred A van der Donk
Department of Chemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, IL 61801, USA
Curr Opin Biotechnol 14:421-6. 2003..The use of electrochemical methods has allowed cofactor regeneration for monooxygenases and natural or engineered whole-cell systems provide alternatives to approaches relying on purified enzymes...
- Dysfunction of mitochondria in human skeletal muscle in type 2 diabetesDavid E Kelley
Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
Diabetes 51:2944-50. 2002..01). We conclude that there is an impaired bioenergetic capacity of skeletal muscle mitochondria in type 2 diabetes, with some impairment also present in obesity...
- Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesisDe Yu Xie
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Science 299:396-9. 2003..Ectopic expression of BAN in tobacco flower petals and Arabidopsis leaves results in loss of anthocyanins and accumulation of CTs...
- GW112, a novel antiapoptotic protein that promotes tumor growthXiuwu Zhang
Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710, USA
Cancer Res 64:2474-81. 2004..Taken together, our data suggest that GW112 is an important regulator of cell death that plays important roles in tumor cell survival and tumor growth...
- Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolyticaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
Eukaryot Cell 1:181-90. 2002..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
- The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3Jun Zhang
Greenebaum Cancer Center, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Proc Natl Acad Sci U S A 100:9342-7. 2003..Because constitutively active STAT3 up-regulates antiapoptotic genes to promote tumor survival, its inhibition by GRIM-19 also demonstrates an antioncogenic effect exerted by biological therapeutics...
- GRIM-19, a death-regulatory gene product, suppresses Stat3 activity via functional interactionChengchen Lufei
Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
EMBO J 22:1325-35. 2003..Our data suggest that GRIM-19 is a novel negative regulator of Stat3...
- Molecular cloning, characterisation and ligand-bound structure of an azoreductase from Pseudomonas aeruginosaChan Ju Wang
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
J Mol Biol 373:1213-28. 2007..The structure of the ligand-bound protein also highlights the pi-stacking interactions between FMN and the azo substrate...
- Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalisZhi Jie Liu
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Arch Biochem Biophys 463:68-77. 2007..A putative NADH binding site could be identified and a plausible mechanism for substrate reduction is proposed. Expression studies revealed azoA gene to be expressed constitutively in E. faecalis...
- GRIM-19, a cell death regulatory protein, is essential for assembly and function of mitochondrial complex IGuochang Huang
Signal Transduction Laboratory, Institute of Molecular and Cell Biology, Proteos Building, Room 6 19B, 61 Biopolis Dr, Singapore 138673, Republic of Singapore
Mol Cell Biol 24:8447-56. 2004..Our result demonstrates that GRIM-19, a gene product with a specific role in IFN-RA-induced cell death, is a functional component of mitochondrial complex I and is essential for early embryonic development...
- Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturationDan Su
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
J Biol Chem 280:26491-8. 2005..Together, TGR and GPx4 can serve as a novel disulfide bond formation system. Both enzymes contain a catalytic selenocysteine consistent with the role of selenium in male reproduction...
- The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat developmentM Devic
Laboratoire de Physiologie et Biologie Moléculaire des Plantes, Universite de Perpignan, Perpignan, France
Plant J 19:387-98. 1999..BAN is probably involved in a metabolic channelling between the production of anthocyanins and pro-anthocyanidins in the seed coat...
- Genetic and epigenetic factors are associated with expression of respiratory chain component NDUFB6 in human skeletal muscleCharlotte Ling
Department of Clinical Sciences, Lund University, University Hospital MAS, Malmo, Sweden
J Clin Invest 117:3427-35. 2007....
- Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systemsQ A Sun
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
Proc Natl Acad Sci U S A 98:3673-8. 2001....
- Preferential inhibition by (-)-epigallocatechin-3-gallate of the cell surface NADH oxidase and growth of transformed cells in cultureD J Morre
Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biochem Pharmacol 60:937-46. 2000..The findings correlate inhibition of cell surface NADH oxidase activity and inhibition of growth with EGCg-induced apoptosis...
- The disulfide redox system of Schistosoma mansoni and the importance of a multifunctional enzyme, thioredoxin glutathione reductaseHeather M Alger
Department of Biological Sciences, Illinois State University, Normal, IL 61790 4120, USA
Mol Biochem Parasitol 121:129-39. 2002..This is the first example of an organism with a redox system based exclusively on thioredoxin glutathione reductase...
- Betraying the parasite's redox system: diaryl sulfide-based inhibitors of trypanothione reductase: subversive substrates and antitrypanosomal propertiesBernhard Stump
Laboratorium fur Organische Chemie, ETH Zurich, Honggerberg, HCI, 8093 Zurich, Switzerland
ChemMedChem 2:1708-12. 2007
- Platyhelminth mitochondrial and cytosolic redox homeostasis is controlled by a single thioredoxin glutathione reductase and dependent on selenium and glutathioneMariana Bonilla
Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Piso 2, Montevideo, Uruguay
J Biol Chem 283:17898-907. 2008..These studies establish the selenium- and glutathione-dependent regulation of cytosolic and mitochondrial redox homeostasis through a single TGR enzyme in platyhelminths...
- Will new antischistosomal drugs finally emerge?Donato Cioli
Institute of Cell Biology, CNR, 32 Via Ramarini, 00015 Monterotondo, Rome, Italy
Trends Parasitol 24:379-82. 2008..This rational and legitimate concern might now begin to be relieved by the recent proposal of a new class of compounds that could represent a novel source of drugs against schistosomiasis...
- Alternative mRNAs arising from trans-splicing code for mitochondrial and cytosolic variants of Echinococcus granulosus thioredoxin Glutathione reductaseAstrid Agorio
Catedra de Inmunologia, Facultad de Química Ciencias, Universidad de la Republica, Avenida Alfredo Navarro 3051, Piso 2, CP 11 600, Montevideo, Uruguay
J Biol Chem 278:12920-8. 2003..granulosus thioredoxin...
- Synthesis and evaluation of 1-(1-(Benzo[b]thiophen-2-yl)cyclohexyl)piperidine (BTCP) analogues as inhibitors of trypanothione reductaseStephen Patterson
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
ChemMedChem 4:1341-53. 2009..Analogues with improved enzymatic and biological activity were obtained. The structure-activity relationships of this novel series are discussed...
- NADH oxidase activity of mitochondrial apoptosis-inducing factorM D Miramar
Departamento de Bioquimica y Biologia Molecular y Celular. Universidad de Zaragoza, Plaza San Francisco s/n 50009 Zaragoza, Spain
J Biol Chem 276:16391-8. 2001..Altogether, these data indicate that AIF has a marked oxidoreductase activity which can be dissociated from its apoptosis-inducing function...
- An AIF orthologue regulates apoptosis in yeastSilke Wissing
Institute for Physiological Chemistry, University of Tubingen, Hoppe Seyler Strasse 4, Tuebingen 72076, Germany
J Cell Biol 166:969-74. 2004..We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1)...
- Somatic and germline mutation in GRIM-19, a dual function gene involved in mitochondrial metabolism and cell death, is linked to mitochondrion-rich (Hurthle cell) tumours of the thyroidV Máximo
IPATIMUP Institute of Molecular Pathology and Immunology of the University of Porto, Rua Dr Roberto Frias s n, 4200 465 Porto, Portugal
Br J Cancer 92:1892-8. 2005....
- Viral interferon regulatory factor 1 of Kaposi's sarcoma-associated herpesvirus interacts with a cell death regulator, GRIM19, and inhibits interferon/retinoic acid-induced cell deathTaegun Seo
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
J Virol 76:8797-807. 2002..Our results collectively indicate that vIRF1 modulates IFN/RA-cell death signals via interactions with GRIM19...
- GRIM-19, a cell death regulatory gene product, is a subunit of bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I)I M Fearnley
Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
J Biol Chem 276:38345-8. 2001..6) is the bovine homolog of GRIM-19, the product of a cell death regulatory gene induced by interferon-beta and retinoic acid, thus providing a new link between the mitochondrion and its electron-transport chain and apoptotic cell death...
- Tumor suppressive protein gene associated with retinoid-interferon-induced mortality (GRIM)-19 inhibits src-induced oncogenic transformation at multiple levelsSudhakar Kalakonda
Department of Microbiology and Immunology, Greenebaum Cancer Center, University of Maryland School of Medicine, 660 West Redwood St, Howard Hall 350, Baltimore, MD 21201, USA
Am J Pathol 171:1352-68. 2007..Thus, GRIM-19 not only blocks src-induced gene expression through STAT3 but also the activation of cell adhesion molecules...
- Mitochondrial DNA somatic mutations (point mutations and large deletions) and mitochondrial DNA variants in human thyroid pathology: a study with emphasis on Hürthle cell tumorsValdemar Máximo
Institute of Molecular Pathology and Immunology, The University of Porto, Porto, Portugal
Am J Pathol 160:1857-65. 2002..Germline polymorphisms of the ATPase 6 gene are associated with the occurrence of mtDNA CD, the hallmark of Hürthle cell tumors...
- Molecular cloning and characterization of a tumor-associated, growth-related, and time-keeping hydroquinone (NADH) oxidase (tNOX) of the HeLa cell surfacePin Ju Chueh
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 41:3732-41. 2002..A single protein with two alternating enzymatic activities indicative of a time-keeping function is unprecedented in the biochemical literature...
- Down-regulation of Leishmania donovani trypanothione reductase by heterologous expression of a trans-dominant mutant homologue: effect on parasite intracellular survivalJ Tovar
Department of Tropical and Infectious Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
Proc Natl Acad Sci U S A 95:5311-6. 1998....
- A virtual screening approach applied to the search for trypanothione reductase inhibitorsD Horvath
CEREP Lille, France
J Med Chem 40:2412-23. 1997..The docking model has also been used to obtain hints about the binding modes of TR ligands...
- Irreversible inactivation of trypanothione reductase by unsaturated Mannich bases: a divinyl ketone as key intermediateBrittany Lee
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 504, 69120 Heidelberg, Germany
J Med Chem 48:7400-10. 2005..Interaction of these compounds with both trypanothione and trypanothione reductase could account for their potent trypanocidal effect against Trypanosoma brucei...
- Reaction mechanism and regulation of mammalian thioredoxin/glutathione reductaseQi An Sun
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 44:14528-37. 2005....
- Ellman's-reagent-mediated regeneration of trypanothione in situ: substrate-economical microplate and time-dependent inhibition assays for trypanothione reductaseChris J Hamilton
Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, Carnelley Building, University of Dundee, Dundee DD1 4HN, UK
Biochem J 369:529-37. 2003..The characterization of a novel time-dependent inhibitor, cis -3-oxo-8,9b-bis-(N(1)-acrylamidospermidyl)-1,2,3,4,4a,9b-hexahydrobenzofuran (PK43), is also described using these procedures...
- Time-dependent inhibitors of trypanothione reductase: analogues of the spermidine alkaloid lunarine and related natural productsChris J Hamilton
Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, Carnelley Building, University of Dundee, Dundee DD1 4HN, UK
Bioorg Med Chem 14:2266-78. 2006..biennis confirms the importance of the unique structure of the tricyclic core as a motif for inhibitor design and reveals that the non-natural enantiomer may be a more suitable scaffold upon which thiophilic groups may be presented...
- Inhibitors of Trypanosoma cruzi trypanothione reductase revealed by virtual screening and parallel synthesisSvea Meiering
Biochemie Zentrum, Universitat Heidelberg, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
J Med Chem 48:4793-802. 2005..Remarkably, all three derivatives carried two copies of an identical 2-methoxy-4-methyl-1-(phenylmethoxy)benzene substituent...
- Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737Yan Bin
School of Environmental and Biological Science and Technology, Dalian University of Technology, 116023, PR China
FEMS Microbiol Lett 236:129-36. 2004..The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage...
- Antitrypanosomal, antileishmanial, and antimalarial activities of quaternary arylalkylammonium 2-amino-4-chlorophenyl phenyl sulfides, a new class of trypanothione reductase inhibitor, and of N-acyl derivatives of 2-amino-4-chlorophenyl phenyl sulfideSeheli Parveen
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, UK
J Med Chem 48:8087-97. 2005..The phenothiazine and diaryl sulfide quaternary compounds were also powerful antimalarials, providing a new structural framework for antimalarial design...
- Isolation and characterization of a putative anthocyanidin reductase gene from Ginkgo bilobaGuo an Shen
Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, PR China
J Plant Physiol 163:224-7. 2006..DNA gel blot analysis indicates that GbANR belongs to a small gene family. Transcription analysis indicates that GbANR is preferentially expressed in leaves...
- Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevisWerner Hummel
Institut für Enzymtechnologie der Heinrich Heine Universität, Forschungszentrum Julich, Wilhelm Johnen Str, D 52426 Jülich, Germany
Biotechnol Lett 25:51-4. 2003..Highest activity was from pH 5.5-7 and at 40 degrees C. The enzyme requires dithiothreitol to prevent oxidative deactivation. The Km value for NADH was 24 microM...
- Two interacting binding sites for quinacrine derivatives in the active site of trypanothione reductase: a template for drug designAhilan Saravanamuthu
Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
J Biol Chem 279:29493-500. 2004....
- Benzofuranyl 3,5-bis-polyamine derivatives as time-dependent inhibitors of trypanothione reductaseChris J Hamilton
Division of Biological Chemistry and Molecular Microbiology, Faculty of Life Sciences, Carnelley Building, University of Dundee, DD1 4HN, Dundee, UK
Bioorg Med Chem 11:3683-93. 2003..The kinetics of time dependent inactivation of trypanothione reductase by 1 and 4 have been determined and are compared and discussed herein...
- 8-Methoxy-naphtho[2,3-b]thiophen-4,9-quinone, a non-competitive inhibitor of trypanothione reductaseCarlos L Zani
Laboratório de Química de Produtos Naturais, Centro de Pesquisas Ren Rachou, FIOCRUZ, Belo Horizonte, MG, 30190 002, Brasil
Mem Inst Oswaldo Cruz 98:565-8. 2003..6 M, respectively. When tested against human glutathione reductase, this compound did not display any significant inhibition at 100 M, indicating a good selectivity against the parasite enzyme...
- Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoproteinHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Road, Jefferson, AR 72079 9502, USA
Microbiology 151:1433-41. 2005..4 microM min(-1) (mg protein)(-1). Azo1 was also able to metabolize Orange II, Amaranth, Ponceau BS and Ponceau S azo dyes. Azo1 represents the first azoreductase to be identified and characterized from human skin microflora...
- Information-based methods in the development of antiparasitic drugsKristina Wolf
4SC AG, Am Klopferspitz 19a, 82152 Martinsried, Germany
Parasitol Res 90:S91-6. 2003..Parasitic targets and antiparasitic compounds studied by various information-based methods include trypanosomal trypanothione reductase, antiprotozoal bisphosphonates, and trypanosomal glycosomal glyceraldehyde-3-phosphate dehydrogenase...
- Trypanosoma cruzi trypanothione reductase inhibitors: phenothiazines and related compounds modify experimental Chagas' disease evolutionH W Rivarola
Cátedra de Física Biomédica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Santa Rosa 1085, 5000 Cordoba, Argentina
Curr Drug Targets Cardiovasc Haematol Disord 2:43-52. 2002..Phenothiazines and related compounds are promising trypanocidal agents for treatment of Chagas' disease. Other trypanocidal agents as nifurtimox, benznidazol,Allopurinol, cystein protease inhibitors and others, are also discussed...
- Cloning and characterization of the gene coding for the aerobic azoreductase from Pigmentiphaga kullae K24S Blumel
Institut fur Mikrobiologie der Universitat Stuttgart, Allmandring 31, 70569, Stuttgart, Germany
Appl Microbiol Biotechnol 62:186-90. 2003..The azoreductase was heterologously expressed in Escherichia coli and shown to convert the sulfonated azo dye Orange I and furthermore Magneson II [4-(4-nitrophenylazo)-1-naphthol]...
- Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed developmentIsabelle Debeaujon
Laboratoire de Biologie des Semences, Unite Mixte de Recherche, 204 Institut National de la Recherche Agronomique, Paris Grignon, 78026 Versailles, France
Plant Cell 15:2514-31. 2003..Importantly, these seeds had no obvious defects in endosperm and embryo development...
- Linked thioredoxin-glutathione systems in platyhelminthsGustavo Salinas
Catedra de Inmunologia, Facultad de Quimica, Instituto de Higiene, Avda A Navarro 3051, Montevideo, CP 11600, Uruguay
Trends Parasitol 20:340-6. 2004..Analysis of published data and expressed-sequence tag databases indicates the presence of linked thioredoxin-glutathione systems in the cytosolic and mitochondrial compartments of these parasites...
- Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalisHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
Protein Expr Purif 34:302-10. 2004..AzoA is the first aerobic azoreductase to be identified and characterized from human intestinal gram-positive bacteria...
- Mechanism and structure-activity relationships of norspermidine-based peptidic inhibitors of trypanothione reductaseMark J Dixon
School of Chemistry, University of Southampton, Southampton SO17 1BJ, UK
Bioorg Med Chem 13:4513-26. 2005....
- Recent advances in azo dye degrading enzyme researchHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
Curr Protein Pept Sci 7:101-11. 2006..In this review, structures and carcinogenicity of azo colorants, protein structure, enzymatic function, and substrate specificity, as well as application of the azo dyes and azoreductases will be discussed...
- Novel alkylpolyaminoguanidines and alkylpolyaminobiguanides with potent antitrypanosomal activityXiangdong Bi
Department of Pharmaceutical Sciences, Wayne State University, 259 Mack Ave, Detroit, MI 48202, USA
Bioorg Med Chem Lett 16:3229-32. 2006..The most effective analogues, 7a, 7b and 8d, inhibited parasitic growth in vitro with IC50 values of 0.18, 0.09 and 0.18 microM, respectively. These agents represent a promising new class of potential antitrypanosomal agents...
- Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganismsKosuke Ito
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
J Biol Chem 281:20567-76. 2006..Furthermore, comparison of the active site structure with that of NQO1 complexed with substrates provides clues to the possible substrate-binding mechanism of AzoR...
- Putative ACP phosphodiesterase gene (acpD) encodes an azoreductaseM Nakanishi
Laboratory of Molecular Biochemistry, Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501 1193, Japan
J Biol Chem 276:46394-9. 2001..These data indicate that the acpD gene product is not acyl carrier protein phosphodiesterase but an azoreductase...
- Mathematical modelling of NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in continuously operated enzyme membrane reactorZvjezdana Findrik
Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c 16, HR 10 000 Zagreb, Croatia
J Biosci Bioeng 104:275-80. 2007..The mathematical model simulations revealed that a high enzyme concentration (up to 30 g cm(-3)) is necessary to obtain and maintain the stationary NADH conversion near 100% for a longer period of time...
- Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysisHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
Microbiology 154:2659-67. 2008..2, 30.6 and 8.2-fold, respectively). However, these mutated enzymes maintained K(m) values similar to the wild-type enzyme. This study provides an insight into the catalytic properties of AzoA in FMN stabilization and enzyme activity...
- Diaryl sulfide-based inhibitors of trypanothione reductase: inhibition potency, revised binding mode and antiprotozoal activitiesBernhard Stump
Laboratorium fur Organische Chemie, ETH Zurich, Honggerberg, HCI, 8093, Zurich, Switzerland
Org Biomol Chem 6:3935-47. 2008..In vitro studies showed IC(50) values in the low micromolar to submicromolar range against Trypanosoma brucei rhodesiense as well as the malaria parasite Plasmodium falciparum...
- Potent and specific inhibitors of trypanothione reductase from Trypanosoma cruzi: bis(2-aminodiphenylsulfides) for fluorescent labeling studiesS Girault
UMR 8525 CNRS, , Institut de Biologie et Institut Pasteur de Lille, France
Bioorg Med Chem 9:837-46. 2001..This transformation might explain the absence of correlation between the potent TR inhibition and the in vitro and in vivo antiparasitic activity with both of the first generation of 2-aminodiphenylsulfides...
- Trypanothione reductase high-throughput screening campaign identifies novel classes of inhibitors with antiparasitic activityGeorgina A Holloway
The Walter and Eliza Hall Institute of Medical Research Biotechnology Centre, 1G Royal Parade, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Campus, 2 Parkville, VIC 3052, Australia
Antimicrob Agents Chemother 53:2824-33. 2009..This paper explores the process of identifying, investigating, and evaluating a series of hits from a high-throughput screening campaign...
- Polyamines with N-(3-phenylpropyl) substituents are effective competitive inhibitors of trypanothione reductase and trypanocidal agentsZ Li
Department of Chemistry, Indiana State University, Terre Haute 47809, USA
Bioorg Med Chem Lett 11:251-4. 2001..The most effective inhibitor studied was compound 12 with a Ki value of 0.151 microM. Six of the compounds described are also effective trypanocides with IC50 values < 1 microM...
- 2- and 3-substituted 1,4-naphthoquinone derivatives as subversive substrates of trypanothione reductase and lipoamide dehydrogenase from Trypanosoma cruzi: synthesis and correlation between redox cycling activities and in vitro cytotoxicityL Salmon-Chemin
, Institut de Biologie de Lille et Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
J Med Chem 44:548-65. 2001..brucei and T. cruzi cultures. The results obtained here confirm that reduction of NQs by parasitic flavoenzymes is a promising strategy for the development of new trypanocidal drugs...
- Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soilY Suzuki
Departments of Pathology, Food Microbiology, and Environmental Sanitation, Osaka Prefectural Institute of Public Health, 1 3 69, Nakamichi, Higashinari ku, Osaka 537 0025, Japan
J Biol Chem 276:9059-65. 2001..This is the first report describing the sequencing and characterization of a gene encoding the azo dye-reducing enzyme, azoreductase, from aerobic bacteria and its expression in E. coli...
- Synthesis and evaluation of 9,9-dimethylxanthene tricyclics against trypanothione reductase, Trypanosoma brucei, Trypanosoma cruzi and Leishmania donovaniK Chibale
Department of Chemistry, University of Cape Town, South Africa
Bioorg Med Chem Lett 10:1147-50. 2000..02, 0.48 and 0.32 microM, for Trypanosoma brucei, Trypanosoma cruzi, and Leishmania donovani, respectively). The lack of correlation between inhibitory activity against TR and ED50 values suggests that TR is not the target...
- The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2FW A van den Berg
Department of Biomolecular Sciences, Wageningen University, The Netherlands
Eur J Biochem 267:666-76. 2000..The protein is only detected in the facultative anaerobes E. coli and Morganella morganii after cultivation under anaerobic conditions in the presence of nitrate or nitrite, suggesting a role in nitrate-and/or nitrite respiration...
- Improved tricyclic inhibitors of trypanothione reductase by screening and chemical synthesisJohn L Richardson
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
ChemMedChem 4:1333-40. 2009..brucei (EC(50)=775 nM)...
- Metabolic consequences of the cytochrome c oxidase deficiency in brain of copper-deficient Mo(vbr) miceW S Kunz
Department of Epileptology, University of Bonn Medical Center, Germany
J Neurochem 72:1580-5. 1999..Owing to the reserve capacity of respiratory chain enzymes, the reported changes in activities do not seem to affect whole-brain high-energy phosphates, as observed in a previous study using 31P NMR...
- Azoreductase from Rhodobacter sphaeroides AS1.1737 is a flavodoxin that also functions as nitroreductase and flavin mononucleotide reductaseGuangfei Liu
School of Environmental and Biological Science and Technology, Dalian University of Technology, 116024 Dalian, People s Republic of China
Appl Microbiol Biotechnol 76:1271-9. 2007..AZR with broad substrate specificity is a member of a new nitro/FMN reductase family demonstrating potential application in bioremediation...
- Polyamine derivatives as inhibitors of trypanothione reductase and assessment of their trypanocidal activitiesM C O'Sullivan
Department of Chemistry, Indiana State University, Terre Haute 47809, USA
Bioorg Med Chem 5:2145-55. 1997..However, these compounds did not prolong the lives of mice infected with trypanosomes. This work indicates that certain polyamine derivatives which target a unique pathway in Trypanosomatidae have potential as antitrypanosomal agents...
- High-throughput screening affords novel and selective trypanothione reductase inhibitors with anti-trypanosomal activityDerek C Martyn
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
Bioorg Med Chem Lett 17:1280-3. 2007..Thirteen compounds from these four chemotypes were purchased, and their in vitro activity against TR and Trypanosoma brucei is described...
- Peptoid inhibition of trypanothione reductase as a potential antitrypanosomal and antileishmanial drug leadC Chan
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, U K
Amino Acids 22:297-308. 2002....
- An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp.: functional expression and enzymatic characterizationToshihiko Ooi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, 060 8628, Sapporo, Japan
Appl Microbiol Biotechnol 75:377-86. 2007..The enzyme was not only able to decolorize MR but also able to decolorize sulfonated azo dyes such as Orange I and Acid Red 88...
- Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46FSilke Blümel
Institut fur Mikrobiologie der Universitat Stuttgart, 70569 Stuttgart, Germany
Appl Environ Microbiol 68:3948-55. 2002..The turnover of several industrially relevant azo dyes by cell extracts from the recombinant E. coli strain was demonstrated...
- Discovery of 2-iminobenzimidazoles as a new class of trypanothione reductase inhibitor by high-throughput screeningGeorgina A Holloway
The Walter and Eliza Hall Institute of Medical Research, Biotechnology Centre, 4 Research Avenue, La Trobe Research and Development Park, Bundoora, VIC 3086, Australia
Bioorg Med Chem Lett 17:1422-7. 2007..These 2-iminobenzimidazoles display potent trypanocidal activity against Trypanosoma brucei rhodesiense, do not inhibit closely related human glutathione reductase and have low cytotoxicity against mammalian cells...
- Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug targetAngela N Kuntz
Department of Biological Sciences, Illinois State University, Normal, Illinois, United States of America
PLoS Med 4:e206. 2007..Our goal was to investigate the potential of a unique, selenium-containing parasite enzyme thioredoxin glutathione reductase (TGR) as a drug target...
- Enzymes of the trypanothione metabolism as targets for antitrypanosomal drug developmentArmin Schmidt
Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, Heidelberg, 69120, Germany
Curr Top Med Chem 2:1239-59. 2002..The most effective inhibitors of the enzymes known to date, their mode of action, and the (dis)advantages of different types of inhibitors as potential drug candidates will be discussed...
- Biosynthesis of flavan 3-ols by leucoanthocyanidin 4-reductases and anthocyanidin reductases in leaves of grape (Vitis vinifera L.), apple (Malus x domestica Borkh.) and other cropsJudith Pfeiffer
Chair of Floriculture Crops and Horticultural Plant Breeding, Technical University Munich, Am Hochanger 4, 85350 Freising, Germany
Plant Physiol Biochem 44:323-34. 2006..ANR enzyme activity was demonstrated for a number of other crop plants and its correlation with (-)-epicatechin and obvious competition with UDP-glycosyl:flavonoid-3-O-glycosyltransferases was considered...
- Novel antitrypanosomal agents based on palladium nitrofurylthiosemicarbazone complexes: DNA and redox metabolism as potential therapeutic targetsLucia Otero
Departamento Estrella Campos, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay
J Med Chem 49:3322-31. 2006..Moreover, the complexes were found to be irreversible inhibitors of trypanothione reductase...
- A drug-unresponsive and protease-resistant CNOX protein from human seraD Sedlak
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
Arch Biochem Biophys 386:106-16. 2001..5- to 24-kDa proteins of human sera, human lymphocytes, and plasma membranes from Escherichia coli with the molecular weight depending on source and conditions of treatment with proteinase K...
- Comparative enzymatic analysis of azoreductases from Bacillus sp. B29Toshihiko Ooi
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan
Biosci Biotechnol Biochem 73:1209-11. 2009..Other enzymatic properties, including the substrate specificities of both azoreductases, were also investigated...
- Cell surface NADH oxidases (ECTO-NOX proteins) with roles in cancer, cellular time-keeping, growth, aging and neurodegenerative diseasesD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, West Lafayette, IN, USA
Free Radic Res 37:795-808. 2003..Period length is independent of temperature (temperature compensated) and synchrony is achieved through entrainment...
- Evaluation of enzymatic assays and compounds affecting ATP production in mitochondrial respiratory chain complex I deficiencyAnn Saada
Metabolic Disease Unit, Shaare Zedek Medical Center, POB 3235, Jerusalem 91031, Israel
Anal Biochem 335:66-72. 2004..The NADH-ferricyanide reductase assay play a helpful role in directing mutation analysis and identifying patients which are more likely to have their cells amenable for ATP production assessment...
- Mode of inhibitory action of Deltalac-acetogenins, a new class of inhibitors of bovine heart mitochondrial complex IMasatoshi Murai
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
Biochemistry 45:9778-87. 2006..The unique inhibitory action of hydrophobic Deltalac-acetogenins may be closely associated with the dynamic function of the membrane domain of complex I...
- Psi-screen, an in vitro toxicity test system: applications in the bioassay of perfumes and fragrance chemicalsDavid E Griffiths
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
Altern Lab Anim 33:471-86. 2005..Twenty candidate fragrance chemicals were investigated and all inhibited Complex I (5 at <35 microM). Mass screening strategies and high-throughput screening assays are discussed...
- Cell activating capacity of 50 Hz magnetic fields to release reactive oxygen intermediates in human umbilical cord blood-derived monocytes and in Mono Mac 6 cellsMadeleine Lupke
University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Albert Einstein Str 3, D 18059 Rostock, Germany
Free Radic Res 38:985-93. 2004..Therefore, we suggest that ELF-MF exposure induces the activation of NADH oxidase in these cells. However, the MF-effect was inhibited by DPI in monocytes, indicating the activation of the NADPH oxidase after exposure to ELF-MF...
- HQNO-sensitive NADH:quinone oxidoreductase of Bacillus cereus KCTC 3674Jiwon Kang
Department of Microbiology, Changwon National University, Sarim dong, Changwon, Kyungnam 641 773, Korea
J Biochem Mol Biol 40:53-7. 2007..cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor...
- Reactive oxygen species generation at the plasma membrane for antibody controlF L Crane
Department of Biological Science, Purdue University, W Lafayette, IN, USA
Autoimmun Rev 7:518-22. 2008..The specific controls of the oxidases in different tissues allow a basis for localized autoantibody modification in response to stress or environment...
- Decylubiquinol impedes mitochondrial respiratory chain complex I activityPaule Benit
INSERM, U676, Hopital Robert Debre, Bât Ecran, Paris, France
Mol Cell Biochem 314:45-50. 2008..Use of these conditions is however restricted to tissues/cells with limited contaminating NADH dehydrogenase activities that are prone to react with redox active compounds...
- Periodic fluctuations in oxygen consumption comparing HeLa (cancer) and CHO (non-cancer) cells and response to external NAD(P)+/NAD(P)HJohn Orczyk
Department of Medicinal Chemistry, Purdue University, West Lafayette, Indiana 47907 2064, USA
Mol Cell Biochem 273:161-7. 2005....
- S-nitrosation of mitochondrial complex I depends on its structural conformationAlexander Galkin
Wolfson Institute for Biomedical Research, University College London, London, UK
J Biol Chem 282:37448-53. 2007..The implications of this finding for hypoxic or pathophysiological conditions in vivo are discussed...
- AIF suppresses chemical stress-induced apoptosis and maintains the transformed state of tumor cellsAlexander Urbano
Cell Death and Human Disease Group, Institute of Molecular and Cell Biology, Proteos, Republic of Singapore
EMBO J 24:2815-26. 2005..AIF maintains the transformed state of colon cancer cells through its NADH oxidase activity, by mechanisms that involve complex I function. On both counts, AIF represents a novel type of cancer drug target...
- The nuclear ABC1 gene is essential for the correct conformation and functioning of the cytochrome bc1 complex and the neighbouring complexes II and IV in the mitochondrial respiratory chainG Brasseur
Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR9036, Institut de Biologie Structurale et Microbiologie, CNRS, Marseille, France
Eur J Biochem 246:103-11. 1997....
- Vascular NADH oxidase is involved in impaired endothelium-dependent vasodilation in OLETF rats, a model of type 2 diabetesYong K Kim
Department of Internal Medicine and Pharmacology, College of Medicine, Pusan National University, Pusan, South Korea
Diabetes 51:522-7. 2002..These findings suggest that upregulated expression of p22phox mRNA and enhanced NADH oxidase activity contribute to the impaired endothelium-dependent vasodilation in OLETF rats...
- Mitochondrial DNA and respiratory chain function in spinal cords of ALS patientsFalk R Wiedemann
Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, USA
J Neurochem 80:616-25. 2002....
- Melatonin increases the activity of the oxidative phosphorylation enzymes and the production of ATP in rat brain and liver mitochondriaMiguel Martin
Departamento de Fisiologia, Instituto de Biotecnologia, Universidad de Granada, E 18012 Granada, Spain
Int J Biochem Cell Biol 34:348-57. 2002..These results provide new hormonal mechanism regulating mitochondrial homeostasis and may explain, at least in part, the anti-aging and neuroprotective properties of melatonin...
- GD3 recruits reactive oxygen species to induce cell proliferation and apoptosis in human aortic smooth muscle cellsAnil Kumar Bhunia
Department of Pediatrics, Lipid Research Atherosclerosis Unit, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21044, USA
J Biol Chem 277:16396-402. 2002..These observations might have relevance in regard to the potential role of GD3 in aortic smooth muscle cell proliferation and apoptosis that may contribute to plaque rupture in atherosclerosis...
- Oxidative phosphorylation enzyme complexes in caloric restrictionAbdullah Olgun
Department of Biochemistry and Clinical Biochemistry, Gulhane School of Medicine, Etlik 06018, Ankara, Turkey
Exp Gerontol 37:639-45. 2002....
- Generation of reactive oxygen species by the mitochondrial electron transport chainYuanbin Liu
Cellular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 9062, USA
J Neurochem 80:780-7. 2002..These new insights clarify an elusive target for intervening mitochondrial ROS-related processes or diseases...
- Structure/Function Studies on FlavoproteinsIrina Sevrioukova; Fiscal Year: 2007..unreadable] [unreadable]..
- Antioxidant Functions of Lipoic AcidJames May; Fiscal Year: 2004..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
- TUMOR GROWTH INHIBITION BY INTERFERON B AND RETINOIDSDHAN KALVAKOLANU; Fiscal Year: 2001..This proposal is a translational study that provides a basis and the necessary reagents for future phase II trials with IFN/RA or other drug combinations for cancer therapy. ..
- Profiling of Redox-Sensitive Signaling ProteinsLeslie Poole; Fiscal Year: 2006..These tools will usher in a new era of redox proteomics and enable proteome-scale studies of effects of oxidative stress and antioxidant therapies on cell pathways and signaling networks. [unreadable] [unreadable] [unreadable]..
- Antioxidant Interaction of Selenium and Vitamins C & EJames May; Fiscal Year: 2008..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
- REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSINGMichael S Wolin; Fiscal Year: 2010..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
- PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATIONMichael S Wolin; Fiscal Year: 2010....
- Antioxidant vitamins in models of Alzheimer's DiseaseJames May; Fiscal Year: 2008..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
- Microfilaments in Giardia Attachment and VirulenceHEIDI ELMENDORF; Fiscal Year: 2006..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
- MECHANISTIC STUDIES ON PHOSPHORUS REDOX ENZYMESWILLEM VAN DER DONK; Fiscal Year: 2006..The studies on HtxA focus on potential use of alternative substrates as well as developing a fundamental understanding of the mode of catalysis. ..
- BIOSYNTHESIS OF SOME MICROBIAL METABOLITESRonald Parry; Fiscal Year: 2007..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
- Role of Oxidatve Stress in Chronic Beryllium DiseaseBrian Day; Fiscal Year: 2006..These studies have the potential to further define the etiology of CBD, risk factors, and suggest novel approaches to prevent and treat this disease. ..
- T CELL ANTIGEN RECOGNITIONEllis L Reinherz; Fiscal Year: 2010..abstract_text> ..
- GENES ENCODING T CELL RECEPTORSEllis Reinherz; Fiscal Year: 2006..Third, the molecular mechanism by which the CD2 cytoplasmic tail interacting proteins CD2BP1, CD2BP3 and CD2BP4 affect cell motility, polarity and lipid biogenesis will be investigated. ..
- 600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETERDaniel Sem; Fiscal Year: 2005..It will also better leverage the use of regional facilities for follow-up refinement studies, rather than routine early-stage experiments. ..
- Transcriptional Regulation in Giardia lambliaHEIDI ELMENDORF; Fiscal Year: 2006..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
- Signaling for cardioprotection against oxidative stressYuichiro Suzuki; Fiscal Year: 2006..In addition, it is expected that the results will fundamentally advance the field of cardiac muscle cell biology. ..
- MACROPHAGES, DENDRITIC CELLS AND PATHOGENSCarl Nathan; Fiscal Year: 2007..Cores provide for BSL3 wet lab and mouse work; microarray analysis of gene expression; computation and comparison of gene expression results; and high throughput screening of chemical libraries. ..
- Glutathione Peroxidase & Redox State in AtherosclerosisFrancis Miller; Fiscal Year: 2009..Information gained from these studies will provide a foundation for additional studies of redox status in vascular disease and potential novel therapeutic strategies to modify the progression of atherosclerosis in patients. ..
- Collaborative Development of a Vaccine against Cutaneous and Visceral LeishmaniasMARY EDYTHE WILSON; Fiscal Year: 2010..Through a coordinated approach in the three laboratories, we will systematically perform pre-clinical testing against several Leishmania species, with the goal of developing a pan-leishmania protective vaccine. ..
- Mechanism of apoptosis in lung vascular smooth muscleYUICHIRO JUSTIN SUZUKI; Fiscal Year: 2010..These results will be significant because they are expected to provide new agents against pulmonary hypertension. In addition, the results will fundamentally advance the field of lung biology. ..
- MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTSTakao Yagi; Fiscal Year: 2009..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
- The mechanism of MPTP resistance in synuclein null mice.William Dauer; Fiscal Year: 2008..This proposal exemplifies the type of clinically related fundamental neurobiological research I plan to pursue during my career. ..
- Mucin-degrading microflora for prophylactic antibioticsROBERT CARMAN; Fiscal Year: 2008..difficile, and reduces the incidence of C. difficile- induced antibiotic-associated diarrhea. [unreadable] [unreadable] [unreadable]..
- T CELL ANTIGEN RECOGNITIONEllis Reinherz; Fiscal Year: 2008..The completion of these experiments will be instrumental in defining specific modes of movement and illuminating the structural mechanism by which TCR signaling is initiated. ..
- Development of therapies to retard Parkinson's diseaseTakao Yagi; Fiscal Year: 2008..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
- NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANSTakao Yagi; Fiscal Year: 2011..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
- 2007 Vitamin B12 and Corphins Gordon ConferenceWILLEM VAN DER DONK; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Thermodynamic Properties of NADH:Nitrate ReductaseMichael Barber; Fiscal Year: 2005..These studies will enhance our understanding of important structure-function relationships in NR and other related metalloflavoproteins. ..
- Mitochondrial control of neuronal excitotoxicityDavid Nicholls; Fiscal Year: 2005..4. To test the hypothesis that mitochondria at specific intracellular locations may be selectively vulnerable to glutamate excitotoxicity because of their proximity to NMDA receptors, or their location in regions of high-energy demand. ..
- The Major Surface Protease of Trypanosomatid ProtozoaMary Wilson; Fiscal Year: 2003..chagasi whose coding sequences predict they lack the usual GPI anchor, and to contrast the amino acid sequences that regulate the intracellular trafficking of these unusual MSPs with the MSPs that possess a GPI membrane anchor. ..
- UBIQUINONE AND MITOCHONDRIAL OXIDATIVE DISORDERS OF AGINManuchair Ebadi; Fiscal Year: 2003..The completion of these studies will undoubtedly provide additional items of information on reactive oxygen species-induced damage of mitochondrial DNA and the protective effects of coenzyme Q10(ubiquinol) to minimize it. ..
- STRUCTURE AND MECHANISM OF FLAVOPROTEIN HYDROXYLASESShiao Chun Tu; Fiscal Year: 2003..The proposed investigations on luciferase will impact on fields beyond the immediate area of flavoprotein hydroxylases. ..
- PINEAL OPIOID RECEPTORS & ANALGESIC ACTION OF MELATONINManuchair Ebadi; Fiscal Year: 2002..Therefore, future research must synthesize highly efficacious melatonin analogues capable of providing maximum analgesia and hopefully being devoid of addiction liability now associated with currently available narcotics. ..
- SIGNAL TRANSDUCTION SYSTEMS IN ALZHEIMERS DISEASEGary Gibson; Fiscal Year: 2003..abstract_text> ..
- NITROGENASE MECHANISM AND ASSEMBLYThomas Poulos; Fiscal Year: 2002..These studies are expected to have an important impact on our understanding of the general issue of how preformed metal clusters can be inserted into proteins. ..
- 7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEINThomas Poulos; Fiscal Year: 2002..g. superoxide, NO). ..
- PATHOPHYSIOLOGY IN NEURODEGENERATIONRussell Swerdlow; Fiscal Year: 2002....
- HYDROXYNONENAL MODIFICATION OF SUPEROXIDE DISMUTASEKenneth Hensley; Fiscal Year: 2002..abstract_text> ..
- BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDSRonald Parry; Fiscal Year: 2001..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
- GP63 EXPRESSION IN LEISHMANIA CHAGASIMary Wilson; Fiscal Year: 2001..They will also provide a model for differential expression of tandemly linked genes in this group of protozoa. ..
- Thymic vaccination: Manipulating the T cell repertoireEllis Reinherz; Fiscal Year: 2003..T cell protection resulting from generation of new TCR specificities against viral determinants not previously recognized by B6 mice should be demonstrable. ..
- MYCOBACTERIAL RESISTANCE TO REACTIVE NITROGEN/OXYGENCarl Nathan; Fiscal Year: 2003....
- Immunogenetics of Leishmania chagasi InfectionMary Wilson; Fiscal Year: 2005..chagasi infection, and to identify specific alleles that influence human immune responses and thereby modify the course of disease. ..
- Dihydrolipoamide Acyltransferase:Target for ChemotherapyCarl Nathan; Fiscal Year: 2004..abstract_text> ..
- Mitochondiral enzymes/oxidative stress in Alzheimer'sGary Gibson; Fiscal Year: 2004..These models will also provide systems to test the efficacy of approaches to limit or reverse the changes in mitochondria. ..
- C. difficile Toxin Membrane Test with Magnetic ParticlesROBERT CARMAN; Fiscal Year: 2004..perfringens enterotoxin, another cause of AAD. The technology developed in this project will be widely applicable for the development of new highly sensitive stool antigen tests. ..
- TARGETING DEFENSES OF MYCOBACTERIUM TUBERCULOSISCarl Nathan; Fiscal Year: 2004..abstract_text> ..
- MOLEC GENETICS OF COMPLEX I IN MAMMALIAN MITOCHONDRIAImmo Scheffler; Fiscal Year: 2004..Such mice promise to become model systems for mitochondrial diseases. 5) Known cDNAs will be tested to identify the defective gene in the other four complementation groups with complex I defects. ..
- Mitochondria and regulation of HIF-1Faton Agani; Fiscal Year: 2004....
- POSITIVE SELECTION OF THYMOCYTESEllis Reinherz; Fiscal Year: 2004..Fourth, the potential role for germline Valpha genes in positive selection will be assessed, with emphasis on CDR1 as suggested by x-ray analysis of N15- VSV8/K/b co-crystals. ..
- Multifunctional Antioxidants as Anti-Cataract AgentsPeter Kador; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
- Investigating the Molecular Mechanism of Hexose-induced Stress in Lens and RetinaPeter F Kador; Fiscal Year: 2010..Moreover, these studies should help identify new drug targets for the treatment of these diabetic complications. ..
- Antioxidants and oxidative damage in mitochondriaCecilia Giulivi; Fiscal Year: 2002..The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function. ..
- Antioxidants and oxidative damage in mitochondriaCecilia Giulivi; Fiscal Year: 2004..Structure-activity association of naturally occurring flavonoids in the abrogation of protein nitration, and 4. Bioavailability of flavonoids containing diets in nitrative stress. ..
- STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHSAh Lim Tsai; Fiscal Year: 2008..The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. [unreadable] [unreadable]..
- Structure-Function and Reacation Mechanism of eNOSAh Lim Tsai; Fiscal Year: 2007..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
- Estrogen Receptor-related Receptor Action in ZebrafishAnn Tarrant; Fiscal Year: 2007..unreadable] [unreadable]..
- Regulation of Mitochondrial Nitric-oxide SynthaseCecilia Giulivi; Fiscal Year: 2003..Studying the biochemistry of mtNOS will provide a better understanding of the role of nitric oxide in the cell-mitochondria interactions relevant to physiological situations. ..
- Magnetic Circular Dichroism/Circular Discroism SystemAh Lim Tsai; Fiscal Year: 2008..To guarantee a reliable MCD/CD facility for ongoing research and training purposes, we chose to buy JASCO spectrometers for their versatile performance, durability and ease of use. [unreadable] [unreadable] [unreadable]..
- Endothelial Specific Caveolin-1 OverexpressionPHILIP BAUER; Fiscal Year: 2003....
- TGF-BETA AND IMMUNOSUPPRESSION IN ORGAN TRANSPLANTATIONAshwani Khanna; Fiscal Year: 2002..These studies will directly determine the role (if any) of the TGF-B and/or p21 pathways in the efficacy, and TGF-B in the toxicity, induced by CsA and tacrolimus. ..