nadph oxidoreductases nadh

Summary

Summary: A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6.

Top Publications

  1. pmc Redox-dependent change of nucleotide affinity to the active site of the mammalian complex I
    Vera G Grivennikova
    Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
    Biochemistry 46:10971-8. 2007
  2. ncbi NAD(P)H oxidase activity in cultured human podocytes: effects of adenosine triphosphate
    S Greiber
    Abteilung Neprologie, Universitätsklink Freiburg, Germany
    Kidney Int 53:654-63. 1998
  3. ncbi The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat
    Nathalie Nesi
    UMR118 INRA Agrocampus Ouest Université de Rennes1 Amélioration des Plantes et Biotechnologies Végétales, Le Rheu Cedex, France
    Plant Cell Rep 28:601-17. 2009
  4. ncbi Recent developments in pyridine nucleotide regeneration
    Wilfred A van der Donk
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, IL 61801, USA
    Curr Opin Biotechnol 14:421-6. 2003
  5. ncbi Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes
    David E Kelley
    Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Diabetes 51:2944-50. 2002
  6. ncbi Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis
    De Yu Xie
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Science 299:396-9. 2003
  7. ncbi GW112, a novel antiapoptotic protein that promotes tumor growth
    Xiuwu Zhang
    Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cancer Res 64:2474-81. 2004
  8. pmc Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolytica
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Eukaryot Cell 1:181-90. 2002
  9. pmc The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3
    Jun Zhang
    Greenebaum Cancer Center, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 100:9342-7. 2003
  10. pmc GRIM-19, a death-regulatory gene product, suppresses Stat3 activity via functional interaction
    Chengchen Lufei
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    EMBO J 22:1325-35. 2003

Research Grants

  1. Structure/Function Studies on Flavoproteins
    Irina Sevrioukova; Fiscal Year: 2007
  2. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
  3. TUMOR GROWTH INHIBITION BY INTERFERON B AND RETINOIDS
    DHAN KALVAKOLANU; Fiscal Year: 2001
  4. Profiling of Redox-Sensitive Signaling Proteins
    Leslie Poole; Fiscal Year: 2006
  5. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
  6. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
  7. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
  8. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
  9. Microfilaments in Giardia Attachment and Virulence
    HEIDI ELMENDORF; Fiscal Year: 2006
  10. MECHANISTIC STUDIES ON PHOSPHORUS REDOX ENZYMES
    WILLEM VAN DER DONK; Fiscal Year: 2006

Detail Information

Publications214 found, 100 shown here

  1. pmc Redox-dependent change of nucleotide affinity to the active site of the mammalian complex I
    Vera G Grivennikova
    Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
    Biochemistry 46:10971-8. 2007
    ..Possible models to explain the different titration patterns for the forward and reverse reactions are briefly discussed...
  2. ncbi NAD(P)H oxidase activity in cultured human podocytes: effects of adenosine triphosphate
    S Greiber
    Abteilung Neprologie, Universitätsklink Freiburg, Germany
    Kidney Int 53:654-63. 1998
    ..Activation of NAD(P)H oxidase by ATP might be secondary to increased mRNA expression of the NADPH oxidase subunit gp67phox...
  3. ncbi The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat
    Nathalie Nesi
    UMR118 INRA Agrocampus Ouest Université de Rennes1 Amélioration des Plantes et Biotechnologies Végétales, Le Rheu Cedex, France
    Plant Cell Rep 28:601-17. 2009
    ....
  4. ncbi Recent developments in pyridine nucleotide regeneration
    Wilfred A van der Donk
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, IL 61801, USA
    Curr Opin Biotechnol 14:421-6. 2003
    ..The use of electrochemical methods has allowed cofactor regeneration for monooxygenases and natural or engineered whole-cell systems provide alternatives to approaches relying on purified enzymes...
  5. ncbi Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes
    David E Kelley
    Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Diabetes 51:2944-50. 2002
    ..01). We conclude that there is an impaired bioenergetic capacity of skeletal muscle mitochondria in type 2 diabetes, with some impairment also present in obesity...
  6. ncbi Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis
    De Yu Xie
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Science 299:396-9. 2003
    ..Ectopic expression of BAN in tobacco flower petals and Arabidopsis leaves results in loss of anthocyanins and accumulation of CTs...
  7. ncbi GW112, a novel antiapoptotic protein that promotes tumor growth
    Xiuwu Zhang
    Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cancer Res 64:2474-81. 2004
    ..Taken together, our data suggest that GW112 is an important regulator of cell death that plays important roles in tumor cell survival and tumor growth...
  8. pmc Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolytica
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Eukaryot Cell 1:181-90. 2002
    ..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
  9. pmc The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3
    Jun Zhang
    Greenebaum Cancer Center, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 100:9342-7. 2003
    ..Because constitutively active STAT3 up-regulates antiapoptotic genes to promote tumor survival, its inhibition by GRIM-19 also demonstrates an antioncogenic effect exerted by biological therapeutics...
  10. pmc GRIM-19, a death-regulatory gene product, suppresses Stat3 activity via functional interaction
    Chengchen Lufei
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    EMBO J 22:1325-35. 2003
    ..Our data suggest that GRIM-19 is a novel negative regulator of Stat3...
  11. ncbi Molecular cloning, characterisation and ligand-bound structure of an azoreductase from Pseudomonas aeruginosa
    Chan Ju Wang
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
    J Mol Biol 373:1213-28. 2007
    ..The structure of the ligand-bound protein also highlights the pi-stacking interactions between FMN and the azo substrate...
  12. ncbi Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalis
    Zhi Jie Liu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Arch Biochem Biophys 463:68-77. 2007
    ..A putative NADH binding site could be identified and a plausible mechanism for substrate reduction is proposed. Expression studies revealed azoA gene to be expressed constitutively in E. faecalis...
  13. pmc GRIM-19, a cell death regulatory protein, is essential for assembly and function of mitochondrial complex I
    Guochang Huang
    Signal Transduction Laboratory, Institute of Molecular and Cell Biology, Proteos Building, Room 6 19B, 61 Biopolis Dr, Singapore 138673, Republic of Singapore
    Mol Cell Biol 24:8447-56. 2004
    ..Our result demonstrates that GRIM-19, a gene product with a specific role in IFN-RA-induced cell death, is a functional component of mitochondrial complex I and is essential for early embryonic development...
  14. ncbi Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation
    Dan Su
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 280:26491-8. 2005
    ..Together, TGR and GPx4 can serve as a novel disulfide bond formation system. Both enzymes contain a catalytic selenocysteine consistent with the role of selenium in male reproduction...
  15. ncbi The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development
    M Devic
    Laboratoire de Physiologie et Biologie Moléculaire des Plantes, Universite de Perpignan, Perpignan, France
    Plant J 19:387-98. 1999
    ..BAN is probably involved in a metabolic channelling between the production of anthocyanins and pro-anthocyanidins in the seed coat...
  16. pmc Genetic and epigenetic factors are associated with expression of respiratory chain component NDUFB6 in human skeletal muscle
    Charlotte Ling
    Department of Clinical Sciences, Lund University, University Hospital MAS, Malmo, Sweden
    J Clin Invest 117:3427-35. 2007
    ....
  17. pmc Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems
    Q A Sun
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 98:3673-8. 2001
    ....
  18. ncbi Preferential inhibition by (-)-epigallocatechin-3-gallate of the cell surface NADH oxidase and growth of transformed cells in culture
    D J Morre
    Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
    Biochem Pharmacol 60:937-46. 2000
    ..The findings correlate inhibition of cell surface NADH oxidase activity and inhibition of growth with EGCg-induced apoptosis...
  19. ncbi The disulfide redox system of Schistosoma mansoni and the importance of a multifunctional enzyme, thioredoxin glutathione reductase
    Heather M Alger
    Department of Biological Sciences, Illinois State University, Normal, IL 61790 4120, USA
    Mol Biochem Parasitol 121:129-39. 2002
    ..This is the first example of an organism with a redox system based exclusively on thioredoxin glutathione reductase...
  20. ncbi Betraying the parasite's redox system: diaryl sulfide-based inhibitors of trypanothione reductase: subversive substrates and antitrypanosomal properties
    Bernhard Stump
    Laboratorium fur Organische Chemie, ETH Zurich, Honggerberg, HCI, 8093 Zurich, Switzerland
    ChemMedChem 2:1708-12. 2007
  21. pmc Platyhelminth mitochondrial and cytosolic redox homeostasis is controlled by a single thioredoxin glutathione reductase and dependent on selenium and glutathione
    Mariana Bonilla
    Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Piso 2, Montevideo, Uruguay
    J Biol Chem 283:17898-907. 2008
    ..These studies establish the selenium- and glutathione-dependent regulation of cytosolic and mitochondrial redox homeostasis through a single TGR enzyme in platyhelminths...
  22. ncbi Will new antischistosomal drugs finally emerge?
    Donato Cioli
    Institute of Cell Biology, CNR, 32 Via Ramarini, 00015 Monterotondo, Rome, Italy
    Trends Parasitol 24:379-82. 2008
    ..This rational and legitimate concern might now begin to be relieved by the recent proposal of a new class of compounds that could represent a novel source of drugs against schistosomiasis...
  23. ncbi Alternative mRNAs arising from trans-splicing code for mitochondrial and cytosolic variants of Echinococcus granulosus thioredoxin Glutathione reductase
    Astrid Agorio
    Catedra de Inmunologia, Facultad de Química Ciencias, Universidad de la Republica, Avenida Alfredo Navarro 3051, Piso 2, CP 11 600, Montevideo, Uruguay
    J Biol Chem 278:12920-8. 2003
    ..granulosus thioredoxin...
  24. pmc Synthesis and evaluation of 1-(1-(Benzo[b]thiophen-2-yl)cyclohexyl)piperidine (BTCP) analogues as inhibitors of trypanothione reductase
    Stephen Patterson
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    ChemMedChem 4:1341-53. 2009
    ..Analogues with improved enzymatic and biological activity were obtained. The structure-activity relationships of this novel series are discussed...
  25. ncbi NADH oxidase activity of mitochondrial apoptosis-inducing factor
    M D Miramar
    Departamento de Bioquimica y Biologia Molecular y Celular. Universidad de Zaragoza, Plaza San Francisco s/n 50009 Zaragoza, Spain
    J Biol Chem 276:16391-8. 2001
    ..Altogether, these data indicate that AIF has a marked oxidoreductase activity which can be dissociated from its apoptosis-inducing function...
  26. pmc An AIF orthologue regulates apoptosis in yeast
    Silke Wissing
    Institute for Physiological Chemistry, University of Tubingen, Hoppe Seyler Strasse 4, Tuebingen 72076, Germany
    J Cell Biol 166:969-74. 2004
    ..We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1)...
  27. pmc Somatic and germline mutation in GRIM-19, a dual function gene involved in mitochondrial metabolism and cell death, is linked to mitochondrion-rich (Hurthle cell) tumours of the thyroid
    V Máximo
    IPATIMUP Institute of Molecular Pathology and Immunology of the University of Porto, Rua Dr Roberto Frias s n, 4200 465 Porto, Portugal
    Br J Cancer 92:1892-8. 2005
    ....
  28. pmc Viral interferon regulatory factor 1 of Kaposi's sarcoma-associated herpesvirus interacts with a cell death regulator, GRIM19, and inhibits interferon/retinoic acid-induced cell death
    Taegun Seo
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Virol 76:8797-807. 2002
    ..Our results collectively indicate that vIRF1 modulates IFN/RA-cell death signals via interactions with GRIM19...
  29. ncbi GRIM-19, a cell death regulatory gene product, is a subunit of bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I)
    I M Fearnley
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 276:38345-8. 2001
    ..6) is the bovine homolog of GRIM-19, the product of a cell death regulatory gene induced by interferon-beta and retinoic acid, thus providing a new link between the mitochondrion and its electron-transport chain and apoptotic cell death...
  30. pmc Tumor suppressive protein gene associated with retinoid-interferon-induced mortality (GRIM)-19 inhibits src-induced oncogenic transformation at multiple levels
    Sudhakar Kalakonda
    Department of Microbiology and Immunology, Greenebaum Cancer Center, University of Maryland School of Medicine, 660 West Redwood St, Howard Hall 350, Baltimore, MD 21201, USA
    Am J Pathol 171:1352-68. 2007
    ..Thus, GRIM-19 not only blocks src-induced gene expression through STAT3 but also the activation of cell adhesion molecules...
  31. pmc Mitochondrial DNA somatic mutations (point mutations and large deletions) and mitochondrial DNA variants in human thyroid pathology: a study with emphasis on Hürthle cell tumors
    Valdemar Máximo
    Institute of Molecular Pathology and Immunology, The University of Porto, Porto, Portugal
    Am J Pathol 160:1857-65. 2002
    ..Germline polymorphisms of the ATPase 6 gene are associated with the occurrence of mtDNA CD, the hallmark of Hürthle cell tumors...
  32. ncbi Molecular cloning and characterization of a tumor-associated, growth-related, and time-keeping hydroquinone (NADH) oxidase (tNOX) of the HeLa cell surface
    Pin Ju Chueh
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 41:3732-41. 2002
    ..A single protein with two alternating enzymatic activities indicative of a time-keeping function is unprecedented in the biochemical literature...
  33. pmc Down-regulation of Leishmania donovani trypanothione reductase by heterologous expression of a trans-dominant mutant homologue: effect on parasite intracellular survival
    J Tovar
    Department of Tropical and Infectious Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 95:5311-6. 1998
    ....
  34. ncbi A virtual screening approach applied to the search for trypanothione reductase inhibitors
    D Horvath
    CEREP Lille, France
    J Med Chem 40:2412-23. 1997
    ..The docking model has also been used to obtain hints about the binding modes of TR ligands...
  35. ncbi Irreversible inactivation of trypanothione reductase by unsaturated Mannich bases: a divinyl ketone as key intermediate
    Brittany Lee
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 504, 69120 Heidelberg, Germany
    J Med Chem 48:7400-10. 2005
    ..Interaction of these compounds with both trypanothione and trypanothione reductase could account for their potent trypanocidal effect against Trypanosoma brucei...
  36. ncbi Reaction mechanism and regulation of mammalian thioredoxin/glutathione reductase
    Qi An Sun
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 44:14528-37. 2005
    ....
  37. pmc Ellman's-reagent-mediated regeneration of trypanothione in situ: substrate-economical microplate and time-dependent inhibition assays for trypanothione reductase
    Chris J Hamilton
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, Carnelley Building, University of Dundee, Dundee DD1 4HN, UK
    Biochem J 369:529-37. 2003
    ..The characterization of a novel time-dependent inhibitor, cis -3-oxo-8,9b-bis-(N(1)-acrylamidospermidyl)-1,2,3,4,4a,9b-hexahydrobenzofuran (PK43), is also described using these procedures...
  38. ncbi Time-dependent inhibitors of trypanothione reductase: analogues of the spermidine alkaloid lunarine and related natural products
    Chris J Hamilton
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, Carnelley Building, University of Dundee, Dundee DD1 4HN, UK
    Bioorg Med Chem 14:2266-78. 2006
    ..biennis confirms the importance of the unique structure of the tricyclic core as a motif for inhibitor design and reveals that the non-natural enantiomer may be a more suitable scaffold upon which thiophilic groups may be presented...
  39. ncbi Inhibitors of Trypanosoma cruzi trypanothione reductase revealed by virtual screening and parallel synthesis
    Svea Meiering
    Biochemie Zentrum, Universitat Heidelberg, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
    J Med Chem 48:4793-802. 2005
    ..Remarkably, all three derivatives carried two copies of an identical 2-methoxy-4-methyl-1-(phenylmethoxy)benzene substituent...
  40. ncbi Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737
    Yan Bin
    School of Environmental and Biological Science and Technology, Dalian University of Technology, 116023, PR China
    FEMS Microbiol Lett 236:129-36. 2004
    ..The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage...
  41. ncbi Antitrypanosomal, antileishmanial, and antimalarial activities of quaternary arylalkylammonium 2-amino-4-chlorophenyl phenyl sulfides, a new class of trypanothione reductase inhibitor, and of N-acyl derivatives of 2-amino-4-chlorophenyl phenyl sulfide
    Seheli Parveen
    School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, UK
    J Med Chem 48:8087-97. 2005
    ..The phenothiazine and diaryl sulfide quaternary compounds were also powerful antimalarials, providing a new structural framework for antimalarial design...
  42. ncbi Isolation and characterization of a putative anthocyanidin reductase gene from Ginkgo biloba
    Guo an Shen
    Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, PR China
    J Plant Physiol 163:224-7. 2006
    ..DNA gel blot analysis indicates that GbANR belongs to a small gene family. Transcription analysis indicates that GbANR is preferentially expressed in leaves...
  43. ncbi Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis
    Werner Hummel
    Institut für Enzymtechnologie der Heinrich Heine Universität, Forschungszentrum Julich, Wilhelm Johnen Str, D 52426 Jülich, Germany
    Biotechnol Lett 25:51-4. 2003
    ..Highest activity was from pH 5.5-7 and at 40 degrees C. The enzyme requires dithiothreitol to prevent oxidative deactivation. The Km value for NADH was 24 microM...
  44. pmc Two interacting binding sites for quinacrine derivatives in the active site of trypanothione reductase: a template for drug design
    Ahilan Saravanamuthu
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 279:29493-500. 2004
    ....
  45. ncbi Benzofuranyl 3,5-bis-polyamine derivatives as time-dependent inhibitors of trypanothione reductase
    Chris J Hamilton
    Division of Biological Chemistry and Molecular Microbiology, Faculty of Life Sciences, Carnelley Building, University of Dundee, DD1 4HN, Dundee, UK
    Bioorg Med Chem 11:3683-93. 2003
    ..The kinetics of time dependent inactivation of trypanothione reductase by 1 and 4 have been determined and are compared and discussed herein...
  46. ncbi 8-Methoxy-naphtho[2,3-b]thiophen-4,9-quinone, a non-competitive inhibitor of trypanothione reductase
    Carlos L Zani
    Laboratório de Química de Produtos Naturais, Centro de Pesquisas Ren Rachou, FIOCRUZ, Belo Horizonte, MG, 30190 002, Brasil
    Mem Inst Oswaldo Cruz 98:565-8. 2003
    ..6 M, respectively. When tested against human glutathione reductase, this compound did not display any significant inhibition at 100 M, indicating a good selectivity against the parasite enzyme...
  47. ncbi Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Road, Jefferson, AR 72079 9502, USA
    Microbiology 151:1433-41. 2005
    ..4 microM min(-1) (mg protein)(-1). Azo1 was also able to metabolize Orange II, Amaranth, Ponceau BS and Ponceau S azo dyes. Azo1 represents the first azoreductase to be identified and characterized from human skin microflora...
  48. ncbi Information-based methods in the development of antiparasitic drugs
    Kristina Wolf
    4SC AG, Am Klopferspitz 19a, 82152 Martinsried, Germany
    Parasitol Res 90:S91-6. 2003
    ..Parasitic targets and antiparasitic compounds studied by various information-based methods include trypanosomal trypanothione reductase, antiprotozoal bisphosphonates, and trypanosomal glycosomal glyceraldehyde-3-phosphate dehydrogenase...
  49. ncbi Trypanosoma cruzi trypanothione reductase inhibitors: phenothiazines and related compounds modify experimental Chagas' disease evolution
    H W Rivarola
    Cátedra de Física Biomédica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Santa Rosa 1085, 5000 Cordoba, Argentina
    Curr Drug Targets Cardiovasc Haematol Disord 2:43-52. 2002
    ..Phenothiazines and related compounds are promising trypanocidal agents for treatment of Chagas' disease. Other trypanocidal agents as nifurtimox, benznidazol,Allopurinol, cystein protease inhibitors and others, are also discussed...
  50. ncbi Cloning and characterization of the gene coding for the aerobic azoreductase from Pigmentiphaga kullae K24
    S Blumel
    Institut fur Mikrobiologie der Universitat Stuttgart, Allmandring 31, 70569, Stuttgart, Germany
    Appl Microbiol Biotechnol 62:186-90. 2003
    ..The azoreductase was heterologously expressed in Escherichia coli and shown to convert the sulfonated azo dye Orange I and furthermore Magneson II [4-(4-nitrophenylazo)-1-naphthol]...
  51. pmc Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development
    Isabelle Debeaujon
    Laboratoire de Biologie des Semences, Unite Mixte de Recherche, 204 Institut National de la Recherche Agronomique, Paris Grignon, 78026 Versailles, France
    Plant Cell 15:2514-31. 2003
    ..Importantly, these seeds had no obvious defects in endosperm and embryo development...
  52. ncbi Linked thioredoxin-glutathione systems in platyhelminths
    Gustavo Salinas
    Catedra de Inmunologia, Facultad de Quimica, Instituto de Higiene, Avda A Navarro 3051, Montevideo, CP 11600, Uruguay
    Trends Parasitol 20:340-6. 2004
    ..Analysis of published data and expressed-sequence tag databases indicates the presence of linked thioredoxin-glutathione systems in the cytosolic and mitochondrial compartments of these parasites...
  53. ncbi Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Protein Expr Purif 34:302-10. 2004
    ..AzoA is the first aerobic azoreductase to be identified and characterized from human intestinal gram-positive bacteria...
  54. ncbi Mechanism and structure-activity relationships of norspermidine-based peptidic inhibitors of trypanothione reductase
    Mark J Dixon
    School of Chemistry, University of Southampton, Southampton SO17 1BJ, UK
    Bioorg Med Chem 13:4513-26. 2005
    ....
  55. ncbi Recent advances in azo dye degrading enzyme research
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Curr Protein Pept Sci 7:101-11. 2006
    ..In this review, structures and carcinogenicity of azo colorants, protein structure, enzymatic function, and substrate specificity, as well as application of the azo dyes and azoreductases will be discussed...
  56. ncbi Novel alkylpolyaminoguanidines and alkylpolyaminobiguanides with potent antitrypanosomal activity
    Xiangdong Bi
    Department of Pharmaceutical Sciences, Wayne State University, 259 Mack Ave, Detroit, MI 48202, USA
    Bioorg Med Chem Lett 16:3229-32. 2006
    ..The most effective analogues, 7a, 7b and 8d, inhibited parasitic growth in vitro with IC50 values of 0.18, 0.09 and 0.18 microM, respectively. These agents represent a promising new class of potential antitrypanosomal agents...
  57. ncbi Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms
    Kosuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 281:20567-76. 2006
    ..Furthermore, comparison of the active site structure with that of NQO1 complexed with substrates provides clues to the possible substrate-binding mechanism of AzoR...
  58. ncbi Putative ACP phosphodiesterase gene (acpD) encodes an azoreductase
    M Nakanishi
    Laboratory of Molecular Biochemistry, Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501 1193, Japan
    J Biol Chem 276:46394-9. 2001
    ..These data indicate that the acpD gene product is not acyl carrier protein phosphodiesterase but an azoreductase...
  59. ncbi Mathematical modelling of NADH oxidation catalyzed by new NADH oxidase from Lactobacillus brevis in continuously operated enzyme membrane reactor
    Zvjezdana Findrik
    Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c 16, HR 10 000 Zagreb, Croatia
    J Biosci Bioeng 104:275-80. 2007
    ..The mathematical model simulations revealed that a high enzyme concentration (up to 30 g cm(-3)) is necessary to obtain and maintain the stationary NADH conversion near 100% for a longer period of time...
  60. ncbi Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Microbiology 154:2659-67. 2008
    ..2, 30.6 and 8.2-fold, respectively). However, these mutated enzymes maintained K(m) values similar to the wild-type enzyme. This study provides an insight into the catalytic properties of AzoA in FMN stabilization and enzyme activity...
  61. ncbi Diaryl sulfide-based inhibitors of trypanothione reductase: inhibition potency, revised binding mode and antiprotozoal activities
    Bernhard Stump
    Laboratorium fur Organische Chemie, ETH Zurich, Honggerberg, HCI, 8093, Zurich, Switzerland
    Org Biomol Chem 6:3935-47. 2008
    ..In vitro studies showed IC(50) values in the low micromolar to submicromolar range against Trypanosoma brucei rhodesiense as well as the malaria parasite Plasmodium falciparum...
  62. ncbi Potent and specific inhibitors of trypanothione reductase from Trypanosoma cruzi: bis(2-aminodiphenylsulfides) for fluorescent labeling studies
    S Girault
    UMR 8525 CNRS, , Institut de Biologie et Institut Pasteur de Lille, France
    Bioorg Med Chem 9:837-46. 2001
    ..This transformation might explain the absence of correlation between the potent TR inhibition and the in vitro and in vivo antiparasitic activity with both of the first generation of 2-aminodiphenylsulfides...
  63. pmc Trypanothione reductase high-throughput screening campaign identifies novel classes of inhibitors with antiparasitic activity
    Georgina A Holloway
    The Walter and Eliza Hall Institute of Medical Research Biotechnology Centre, 1G Royal Parade, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Campus, 2 Parkville, VIC 3052, Australia
    Antimicrob Agents Chemother 53:2824-33. 2009
    ..This paper explores the process of identifying, investigating, and evaluating a series of hits from a high-throughput screening campaign...
  64. ncbi Polyamines with N-(3-phenylpropyl) substituents are effective competitive inhibitors of trypanothione reductase and trypanocidal agents
    Z Li
    Department of Chemistry, Indiana State University, Terre Haute 47809, USA
    Bioorg Med Chem Lett 11:251-4. 2001
    ..The most effective inhibitor studied was compound 12 with a Ki value of 0.151 microM. Six of the compounds described are also effective trypanocides with IC50 values < 1 microM...
  65. ncbi 2- and 3-substituted 1,4-naphthoquinone derivatives as subversive substrates of trypanothione reductase and lipoamide dehydrogenase from Trypanosoma cruzi: synthesis and correlation between redox cycling activities and in vitro cytotoxicity
    L Salmon-Chemin
    , Institut de Biologie de Lille et Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
    J Med Chem 44:548-65. 2001
    ..brucei and T. cruzi cultures. The results obtained here confirm that reduction of NQs by parasitic flavoenzymes is a promising strategy for the development of new trypanocidal drugs...
  66. ncbi Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil
    Y Suzuki
    Departments of Pathology, Food Microbiology, and Environmental Sanitation, Osaka Prefectural Institute of Public Health, 1 3 69, Nakamichi, Higashinari ku, Osaka 537 0025, Japan
    J Biol Chem 276:9059-65. 2001
    ..This is the first report describing the sequencing and characterization of a gene encoding the azo dye-reducing enzyme, azoreductase, from aerobic bacteria and its expression in E. coli...
  67. ncbi Synthesis and evaluation of 9,9-dimethylxanthene tricyclics against trypanothione reductase, Trypanosoma brucei, Trypanosoma cruzi and Leishmania donovani
    K Chibale
    Department of Chemistry, University of Cape Town, South Africa
    Bioorg Med Chem Lett 10:1147-50. 2000
    ..02, 0.48 and 0.32 microM, for Trypanosoma brucei, Trypanosoma cruzi, and Leishmania donovani, respectively). The lack of correlation between inhibitory activity against TR and ED50 values suggests that TR is not the target...
  68. ncbi The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2F
    W A van den Berg
    Department of Biomolecular Sciences, Wageningen University, The Netherlands
    Eur J Biochem 267:666-76. 2000
    ..The protein is only detected in the facultative anaerobes E. coli and Morganella morganii after cultivation under anaerobic conditions in the presence of nitrate or nitrite, suggesting a role in nitrate-and/or nitrite respiration...
  69. pmc Improved tricyclic inhibitors of trypanothione reductase by screening and chemical synthesis
    John L Richardson
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    ChemMedChem 4:1333-40. 2009
    ..brucei (EC(50)=775 nM)...
  70. ncbi Metabolic consequences of the cytochrome c oxidase deficiency in brain of copper-deficient Mo(vbr) mice
    W S Kunz
    Department of Epileptology, University of Bonn Medical Center, Germany
    J Neurochem 72:1580-5. 1999
    ..Owing to the reserve capacity of respiratory chain enzymes, the reported changes in activities do not seem to affect whole-brain high-energy phosphates, as observed in a previous study using 31P NMR...
  71. ncbi Azoreductase from Rhodobacter sphaeroides AS1.1737 is a flavodoxin that also functions as nitroreductase and flavin mononucleotide reductase
    Guangfei Liu
    School of Environmental and Biological Science and Technology, Dalian University of Technology, 116024 Dalian, People s Republic of China
    Appl Microbiol Biotechnol 76:1271-9. 2007
    ..AZR with broad substrate specificity is a member of a new nitro/FMN reductase family demonstrating potential application in bioremediation...
  72. ncbi Polyamine derivatives as inhibitors of trypanothione reductase and assessment of their trypanocidal activities
    M C O'Sullivan
    Department of Chemistry, Indiana State University, Terre Haute 47809, USA
    Bioorg Med Chem 5:2145-55. 1997
    ..However, these compounds did not prolong the lives of mice infected with trypanosomes. This work indicates that certain polyamine derivatives which target a unique pathway in Trypanosomatidae have potential as antitrypanosomal agents...
  73. pmc High-throughput screening affords novel and selective trypanothione reductase inhibitors with anti-trypanosomal activity
    Derek C Martyn
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Bioorg Med Chem Lett 17:1280-3. 2007
    ..Thirteen compounds from these four chemotypes were purchased, and their in vitro activity against TR and Trypanosoma brucei is described...
  74. ncbi Peptoid inhibition of trypanothione reductase as a potential antitrypanosomal and antileishmanial drug lead
    C Chan
    School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, U K
    Amino Acids 22:297-308. 2002
    ....
  75. ncbi An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp.: functional expression and enzymatic characterization
    Toshihiko Ooi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, 060 8628, Sapporo, Japan
    Appl Microbiol Biotechnol 75:377-86. 2007
    ..The enzyme was not only able to decolorize MR but also able to decolorize sulfonated azo dyes such as Orange I and Acid Red 88...
  76. pmc Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F
    Silke Blümel
    Institut fur Mikrobiologie der Universitat Stuttgart, 70569 Stuttgart, Germany
    Appl Environ Microbiol 68:3948-55. 2002
    ..The turnover of several industrially relevant azo dyes by cell extracts from the recombinant E. coli strain was demonstrated...
  77. pmc Discovery of 2-iminobenzimidazoles as a new class of trypanothione reductase inhibitor by high-throughput screening
    Georgina A Holloway
    The Walter and Eliza Hall Institute of Medical Research, Biotechnology Centre, 4 Research Avenue, La Trobe Research and Development Park, Bundoora, VIC 3086, Australia
    Bioorg Med Chem Lett 17:1422-7. 2007
    ..These 2-iminobenzimidazoles display potent trypanocidal activity against Trypanosoma brucei rhodesiense, do not inhibit closely related human glutathione reductase and have low cytotoxicity against mammalian cells...
  78. pmc Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug target
    Angela N Kuntz
    Department of Biological Sciences, Illinois State University, Normal, Illinois, United States of America
    PLoS Med 4:e206. 2007
    ..Our goal was to investigate the potential of a unique, selenium-containing parasite enzyme thioredoxin glutathione reductase (TGR) as a drug target...
  79. ncbi Enzymes of the trypanothione metabolism as targets for antitrypanosomal drug development
    Armin Schmidt
    Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, Heidelberg, 69120, Germany
    Curr Top Med Chem 2:1239-59. 2002
    ..The most effective inhibitors of the enzymes known to date, their mode of action, and the (dis)advantages of different types of inhibitors as potential drug candidates will be discussed...
  80. ncbi Biosynthesis of flavan 3-ols by leucoanthocyanidin 4-reductases and anthocyanidin reductases in leaves of grape (Vitis vinifera L.), apple (Malus x domestica Borkh.) and other crops
    Judith Pfeiffer
    Chair of Floriculture Crops and Horticultural Plant Breeding, Technical University Munich, Am Hochanger 4, 85350 Freising, Germany
    Plant Physiol Biochem 44:323-34. 2006
    ..ANR enzyme activity was demonstrated for a number of other crop plants and its correlation with (-)-epicatechin and obvious competition with UDP-glycosyl:flavonoid-3-O-glycosyltransferases was considered...
  81. ncbi Novel antitrypanosomal agents based on palladium nitrofurylthiosemicarbazone complexes: DNA and redox metabolism as potential therapeutic targets
    Lucia Otero
    Departamento Estrella Campos, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay
    J Med Chem 49:3322-31. 2006
    ..Moreover, the complexes were found to be irreversible inhibitors of trypanothione reductase...
  82. ncbi A drug-unresponsive and protease-resistant CNOX protein from human sera
    D Sedlak
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
    Arch Biochem Biophys 386:106-16. 2001
    ..5- to 24-kDa proteins of human sera, human lymphocytes, and plasma membranes from Escherichia coli with the molecular weight depending on source and conditions of treatment with proteinase K...
  83. ncbi Comparative enzymatic analysis of azoreductases from Bacillus sp. B29
    Toshihiko Ooi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 73:1209-11. 2009
    ..Other enzymatic properties, including the substrate specificities of both azoreductases, were also investigated...
  84. ncbi Cell surface NADH oxidases (ECTO-NOX proteins) with roles in cancer, cellular time-keeping, growth, aging and neurodegenerative diseases
    D James Morre
    Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, West Lafayette, IN, USA
    Free Radic Res 37:795-808. 2003
    ..Period length is independent of temperature (temperature compensated) and synchrony is achieved through entrainment...
  85. ncbi Evaluation of enzymatic assays and compounds affecting ATP production in mitochondrial respiratory chain complex I deficiency
    Ann Saada
    Metabolic Disease Unit, Shaare Zedek Medical Center, POB 3235, Jerusalem 91031, Israel
    Anal Biochem 335:66-72. 2004
    ..The NADH-ferricyanide reductase assay play a helpful role in directing mutation analysis and identifying patients which are more likely to have their cells amenable for ATP production assessment...
  86. ncbi Mode of inhibitory action of Deltalac-acetogenins, a new class of inhibitors of bovine heart mitochondrial complex I
    Masatoshi Murai
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochemistry 45:9778-87. 2006
    ..The unique inhibitory action of hydrophobic Deltalac-acetogenins may be closely associated with the dynamic function of the membrane domain of complex I...
  87. ncbi Psi-screen, an in vitro toxicity test system: applications in the bioassay of perfumes and fragrance chemicals
    David E Griffiths
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Altern Lab Anim 33:471-86. 2005
    ..Twenty candidate fragrance chemicals were investigated and all inhibited Complex I (5 at <35 microM). Mass screening strategies and high-throughput screening assays are discussed...
  88. ncbi Cell activating capacity of 50 Hz magnetic fields to release reactive oxygen intermediates in human umbilical cord blood-derived monocytes and in Mono Mac 6 cells
    Madeleine Lupke
    University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Albert Einstein Str 3, D 18059 Rostock, Germany
    Free Radic Res 38:985-93. 2004
    ..Therefore, we suggest that ELF-MF exposure induces the activation of NADH oxidase in these cells. However, the MF-effect was inhibited by DPI in monocytes, indicating the activation of the NADPH oxidase after exposure to ELF-MF...
  89. ncbi HQNO-sensitive NADH:quinone oxidoreductase of Bacillus cereus KCTC 3674
    Jiwon Kang
    Department of Microbiology, Changwon National University, Sarim dong, Changwon, Kyungnam 641 773, Korea
    J Biochem Mol Biol 40:53-7. 2007
    ..cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor...
  90. ncbi Reactive oxygen species generation at the plasma membrane for antibody control
    F L Crane
    Department of Biological Science, Purdue University, W Lafayette, IN, USA
    Autoimmun Rev 7:518-22. 2008
    ..The specific controls of the oxidases in different tissues allow a basis for localized autoantibody modification in response to stress or environment...
  91. ncbi Decylubiquinol impedes mitochondrial respiratory chain complex I activity
    Paule Benit
    INSERM, U676, Hopital Robert Debre, Bât Ecran, Paris, France
    Mol Cell Biochem 314:45-50. 2008
    ..Use of these conditions is however restricted to tissues/cells with limited contaminating NADH dehydrogenase activities that are prone to react with redox active compounds...
  92. ncbi Periodic fluctuations in oxygen consumption comparing HeLa (cancer) and CHO (non-cancer) cells and response to external NAD(P)+/NAD(P)H
    John Orczyk
    Department of Medicinal Chemistry, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Mol Cell Biochem 273:161-7. 2005
    ....
  93. ncbi S-nitrosation of mitochondrial complex I depends on its structural conformation
    Alexander Galkin
    Wolfson Institute for Biomedical Research, University College London, London, UK
    J Biol Chem 282:37448-53. 2007
    ..The implications of this finding for hypoxic or pathophysiological conditions in vivo are discussed...
  94. pmc AIF suppresses chemical stress-induced apoptosis and maintains the transformed state of tumor cells
    Alexander Urbano
    Cell Death and Human Disease Group, Institute of Molecular and Cell Biology, Proteos, Republic of Singapore
    EMBO J 24:2815-26. 2005
    ..AIF maintains the transformed state of colon cancer cells through its NADH oxidase activity, by mechanisms that involve complex I function. On both counts, AIF represents a novel type of cancer drug target...
  95. ncbi The nuclear ABC1 gene is essential for the correct conformation and functioning of the cytochrome bc1 complex and the neighbouring complexes II and IV in the mitochondrial respiratory chain
    G Brasseur
    Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR9036, Institut de Biologie Structurale et Microbiologie, CNRS, Marseille, France
    Eur J Biochem 246:103-11. 1997
    ....
  96. ncbi Vascular NADH oxidase is involved in impaired endothelium-dependent vasodilation in OLETF rats, a model of type 2 diabetes
    Yong K Kim
    Department of Internal Medicine and Pharmacology, College of Medicine, Pusan National University, Pusan, South Korea
    Diabetes 51:522-7. 2002
    ..These findings suggest that upregulated expression of p22phox mRNA and enhanced NADH oxidase activity contribute to the impaired endothelium-dependent vasodilation in OLETF rats...
  97. ncbi Mitochondrial DNA and respiratory chain function in spinal cords of ALS patients
    Falk R Wiedemann
    Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, USA
    J Neurochem 80:616-25. 2002
    ....
  98. ncbi Melatonin increases the activity of the oxidative phosphorylation enzymes and the production of ATP in rat brain and liver mitochondria
    Miguel Martin
    Departamento de Fisiologia, Instituto de Biotecnologia, Universidad de Granada, E 18012 Granada, Spain
    Int J Biochem Cell Biol 34:348-57. 2002
    ..These results provide new hormonal mechanism regulating mitochondrial homeostasis and may explain, at least in part, the anti-aging and neuroprotective properties of melatonin...
  99. ncbi GD3 recruits reactive oxygen species to induce cell proliferation and apoptosis in human aortic smooth muscle cells
    Anil Kumar Bhunia
    Department of Pediatrics, Lipid Research Atherosclerosis Unit, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21044, USA
    J Biol Chem 277:16396-402. 2002
    ..These observations might have relevance in regard to the potential role of GD3 in aortic smooth muscle cell proliferation and apoptosis that may contribute to plaque rupture in atherosclerosis...
  100. ncbi Oxidative phosphorylation enzyme complexes in caloric restriction
    Abdullah Olgun
    Department of Biochemistry and Clinical Biochemistry, Gulhane School of Medicine, Etlik 06018, Ankara, Turkey
    Exp Gerontol 37:639-45. 2002
    ....
  101. ncbi Generation of reactive oxygen species by the mitochondrial electron transport chain
    Yuanbin Liu
    Cellular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 9062, USA
    J Neurochem 80:780-7. 2002
    ..These new insights clarify an elusive target for intervening mitochondrial ROS-related processes or diseases...

Research Grants65

  1. Structure/Function Studies on Flavoproteins
    Irina Sevrioukova; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  2. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  3. TUMOR GROWTH INHIBITION BY INTERFERON B AND RETINOIDS
    DHAN KALVAKOLANU; Fiscal Year: 2001
    ..This proposal is a translational study that provides a basis and the necessary reagents for future phase II trials with IFN/RA or other drug combinations for cancer therapy. ..
  4. Profiling of Redox-Sensitive Signaling Proteins
    Leslie Poole; Fiscal Year: 2006
    ..These tools will usher in a new era of redox proteomics and enable proteome-scale studies of effects of oxidative stress and antioxidant therapies on cell pathways and signaling networks. [unreadable] [unreadable] [unreadable]..
  5. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  6. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
    ..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
  7. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....
  8. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  9. Microfilaments in Giardia Attachment and Virulence
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
  10. MECHANISTIC STUDIES ON PHOSPHORUS REDOX ENZYMES
    WILLEM VAN DER DONK; Fiscal Year: 2006
    ..The studies on HtxA focus on potential use of alternative substrates as well as developing a fundamental understanding of the mode of catalysis. ..
  11. BIOSYNTHESIS OF SOME MICROBIAL METABOLITES
    Ronald Parry; Fiscal Year: 2007
    ..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
  12. Role of Oxidatve Stress in Chronic Beryllium Disease
    Brian Day; Fiscal Year: 2006
    ..These studies have the potential to further define the etiology of CBD, risk factors, and suggest novel approaches to prevent and treat this disease. ..
  13. T CELL ANTIGEN RECOGNITION
    Ellis L Reinherz; Fiscal Year: 2010
    ..abstract_text> ..
  14. GENES ENCODING T CELL RECEPTORS
    Ellis Reinherz; Fiscal Year: 2006
    ..Third, the molecular mechanism by which the CD2 cytoplasmic tail interacting proteins CD2BP1, CD2BP3 and CD2BP4 affect cell motility, polarity and lipid biogenesis will be investigated. ..
  15. 600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER
    Daniel Sem; Fiscal Year: 2005
    ..It will also better leverage the use of regional facilities for follow-up refinement studies, rather than routine early-stage experiments. ..
  16. Transcriptional Regulation in Giardia lamblia
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
  17. Signaling for cardioprotection against oxidative stress
    Yuichiro Suzuki; Fiscal Year: 2006
    ..In addition, it is expected that the results will fundamentally advance the field of cardiac muscle cell biology. ..
  18. MACROPHAGES, DENDRITIC CELLS AND PATHOGENS
    Carl Nathan; Fiscal Year: 2007
    ..Cores provide for BSL3 wet lab and mouse work; microarray analysis of gene expression; computation and comparison of gene expression results; and high throughput screening of chemical libraries. ..
  19. Glutathione Peroxidase & Redox State in Atherosclerosis
    Francis Miller; Fiscal Year: 2009
    ..Information gained from these studies will provide a foundation for additional studies of redox status in vascular disease and potential novel therapeutic strategies to modify the progression of atherosclerosis in patients. ..
  20. Collaborative Development of a Vaccine against Cutaneous and Visceral Leishmanias
    MARY EDYTHE WILSON; Fiscal Year: 2010
    ..Through a coordinated approach in the three laboratories, we will systematically perform pre-clinical testing against several Leishmania species, with the goal of developing a pan-leishmania protective vaccine. ..
  21. Mechanism of apoptosis in lung vascular smooth muscle
    YUICHIRO JUSTIN SUZUKI; Fiscal Year: 2010
    ..These results will be significant because they are expected to provide new agents against pulmonary hypertension. In addition, the results will fundamentally advance the field of lung biology. ..
  22. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2009
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  23. The mechanism of MPTP resistance in synuclein null mice.
    William Dauer; Fiscal Year: 2008
    ..This proposal exemplifies the type of clinically related fundamental neurobiological research I plan to pursue during my career. ..
  24. Mucin-degrading microflora for prophylactic antibiotics
    ROBERT CARMAN; Fiscal Year: 2008
    ..difficile, and reduces the incidence of C. difficile- induced antibiotic-associated diarrhea. [unreadable] [unreadable] [unreadable]..
  25. T CELL ANTIGEN RECOGNITION
    Ellis Reinherz; Fiscal Year: 2008
    ..The completion of these experiments will be instrumental in defining specific modes of movement and illuminating the structural mechanism by which TCR signaling is initiated. ..
  26. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2008
    ..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
  27. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2011
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  28. 2007 Vitamin B12 and Corphins Gordon Conference
    WILLEM VAN DER DONK; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Thermodynamic Properties of NADH:Nitrate Reductase
    Michael Barber; Fiscal Year: 2005
    ..These studies will enhance our understanding of important structure-function relationships in NR and other related metalloflavoproteins. ..
  30. Mitochondrial control of neuronal excitotoxicity
    David Nicholls; Fiscal Year: 2005
    ..4. To test the hypothesis that mitochondria at specific intracellular locations may be selectively vulnerable to glutamate excitotoxicity because of their proximity to NMDA receptors, or their location in regions of high-energy demand. ..
  31. The Major Surface Protease of Trypanosomatid Protozoa
    Mary Wilson; Fiscal Year: 2003
    ..chagasi whose coding sequences predict they lack the usual GPI anchor, and to contrast the amino acid sequences that regulate the intracellular trafficking of these unusual MSPs with the MSPs that possess a GPI membrane anchor. ..
  32. UBIQUINONE AND MITOCHONDRIAL OXIDATIVE DISORDERS OF AGIN
    Manuchair Ebadi; Fiscal Year: 2003
    ..The completion of these studies will undoubtedly provide additional items of information on reactive oxygen species-induced damage of mitochondrial DNA and the protective effects of coenzyme Q10(ubiquinol) to minimize it. ..
  33. STRUCTURE AND MECHANISM OF FLAVOPROTEIN HYDROXYLASES
    Shiao Chun Tu; Fiscal Year: 2003
    ..The proposed investigations on luciferase will impact on fields beyond the immediate area of flavoprotein hydroxylases. ..
  34. PINEAL OPIOID RECEPTORS & ANALGESIC ACTION OF MELATONIN
    Manuchair Ebadi; Fiscal Year: 2002
    ..Therefore, future research must synthesize highly efficacious melatonin analogues capable of providing maximum analgesia and hopefully being devoid of addiction liability now associated with currently available narcotics. ..
  35. SIGNAL TRANSDUCTION SYSTEMS IN ALZHEIMERS DISEASE
    Gary Gibson; Fiscal Year: 2003
    ..abstract_text> ..
  36. NITROGENASE MECHANISM AND ASSEMBLY
    Thomas Poulos; Fiscal Year: 2002
    ..These studies are expected to have an important impact on our understanding of the general issue of how preformed metal clusters can be inserted into proteins. ..
  37. 7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEIN
    Thomas Poulos; Fiscal Year: 2002
    ..g. superoxide, NO). ..
  38. PATHOPHYSIOLOGY IN NEURODEGENERATION
    Russell Swerdlow; Fiscal Year: 2002
    ....
  39. HYDROXYNONENAL MODIFICATION OF SUPEROXIDE DISMUTASE
    Kenneth Hensley; Fiscal Year: 2002
    ..abstract_text> ..
  40. BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDS
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
  41. GP63 EXPRESSION IN LEISHMANIA CHAGASI
    Mary Wilson; Fiscal Year: 2001
    ..They will also provide a model for differential expression of tandemly linked genes in this group of protozoa. ..
  42. Thymic vaccination: Manipulating the T cell repertoire
    Ellis Reinherz; Fiscal Year: 2003
    ..T cell protection resulting from generation of new TCR specificities against viral determinants not previously recognized by B6 mice should be demonstrable. ..
  43. MYCOBACTERIAL RESISTANCE TO REACTIVE NITROGEN/OXYGEN
    Carl Nathan; Fiscal Year: 2003
    ....
  44. Immunogenetics of Leishmania chagasi Infection
    Mary Wilson; Fiscal Year: 2005
    ..chagasi infection, and to identify specific alleles that influence human immune responses and thereby modify the course of disease. ..
  45. Dihydrolipoamide Acyltransferase:Target for Chemotherapy
    Carl Nathan; Fiscal Year: 2004
    ..abstract_text> ..
  46. Mitochondiral enzymes/oxidative stress in Alzheimer's
    Gary Gibson; Fiscal Year: 2004
    ..These models will also provide systems to test the efficacy of approaches to limit or reverse the changes in mitochondria. ..
  47. C. difficile Toxin Membrane Test with Magnetic Particles
    ROBERT CARMAN; Fiscal Year: 2004
    ..perfringens enterotoxin, another cause of AAD. The technology developed in this project will be widely applicable for the development of new highly sensitive stool antigen tests. ..
  48. TARGETING DEFENSES OF MYCOBACTERIUM TUBERCULOSIS
    Carl Nathan; Fiscal Year: 2004
    ..abstract_text> ..
  49. MOLEC GENETICS OF COMPLEX I IN MAMMALIAN MITOCHONDRIA
    Immo Scheffler; Fiscal Year: 2004
    ..Such mice promise to become model systems for mitochondrial diseases. 5) Known cDNAs will be tested to identify the defective gene in the other four complementation groups with complex I defects. ..
  50. Mitochondria and regulation of HIF-1
    Faton Agani; Fiscal Year: 2004
    ....
  51. POSITIVE SELECTION OF THYMOCYTES
    Ellis Reinherz; Fiscal Year: 2004
    ..Fourth, the potential role for germline Valpha genes in positive selection will be assessed, with emphasis on CDR1 as suggested by x-ray analysis of N15- VSV8/K/b co-crystals. ..
  52. Multifunctional Antioxidants as Anti-Cataract Agents
    Peter Kador; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  53. Investigating the Molecular Mechanism of Hexose-induced Stress in Lens and Retina
    Peter F Kador; Fiscal Year: 2010
    ..Moreover, these studies should help identify new drug targets for the treatment of these diabetic complications. ..
  54. Antioxidants and oxidative damage in mitochondria
    Cecilia Giulivi; Fiscal Year: 2002
    ..The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function. ..
  55. Antioxidants and oxidative damage in mitochondria
    Cecilia Giulivi; Fiscal Year: 2004
    ..Structure-activity association of naturally occurring flavonoids in the abrogation of protein nitration, and 4. Bioavailability of flavonoids containing diets in nitrative stress. ..
  56. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2008
    ..The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. [unreadable] [unreadable]..
  57. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2007
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  58. Estrogen Receptor-related Receptor Action in Zebrafish
    Ann Tarrant; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  59. Regulation of Mitochondrial Nitric-oxide Synthase
    Cecilia Giulivi; Fiscal Year: 2003
    ..Studying the biochemistry of mtNOS will provide a better understanding of the role of nitric oxide in the cell-mitochondria interactions relevant to physiological situations. ..
  60. Magnetic Circular Dichroism/Circular Discroism System
    Ah Lim Tsai; Fiscal Year: 2008
    ..To guarantee a reliable MCD/CD facility for ongoing research and training purposes, we chose to buy JASCO spectrometers for their versatile performance, durability and ease of use. [unreadable] [unreadable] [unreadable]..
  61. Endothelial Specific Caveolin-1 Overexpression
    PHILIP BAUER; Fiscal Year: 2003
    ....
  62. TGF-BETA AND IMMUNOSUPPRESSION IN ORGAN TRANSPLANTATION
    Ashwani Khanna; Fiscal Year: 2002
    ..These studies will directly determine the role (if any) of the TGF-B and/or p21 pathways in the efficacy, and TGF-B in the toxicity, induced by CsA and tacrolimus. ..