luciferases

Summary

Summary: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.

Top Publications

  1. ncbi Photonic detection of bacterial pathogens in living hosts
    C H Contag
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402, USA
    Mol Microbiol 18:593-603. 1995
  2. pmc PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
    Seung Hee Yoo
    Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 101:5339-46. 2004
  3. ncbi Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells
    O Tetsu
    University of California, San Francisco, School of Medicine, Cancer Research Institute, 94143 0128, USA
    Nature 398:422-6. 1999
  4. ncbi Entrainment of the circadian clock in the liver by feeding
    K A Stokkan
    National Science Foundation Center for Biological Timing and Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Science 291:490-3. 2001
  5. ncbi Multiple microRNAs modulate p21Cip1/Waf1 expression by directly targeting its 3' untranslated region
    S Wu
    Department of Hematology, Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fuzhou, China
    Oncogene 29:2302-8. 2010
  6. ncbi Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
  7. pmc Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
    Angie M Cheng
    Ambion, Inc 2130 Woodward Street, Austin, TX 78744 1832, USA
    Nucleic Acids Res 33:1290-7. 2005
  8. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
  9. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
  10. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002

Detail Information

Publications300 found, 100 shown here

  1. ncbi Photonic detection of bacterial pathogens in living hosts
    C H Contag
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402, USA
    Mol Microbiol 18:593-603. 1995
    ..In vivo efficacy of an antibiotic was monitored using this optical method. This study demonstrates that real time non-invasive analyses of pathogenic events and pharmacological monitoring can be performed in vivo...
  2. pmc PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
    Seung Hee Yoo
    Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 101:5339-46. 2004
    ..These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level...
  3. ncbi Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells
    O Tetsu
    University of California, San Francisco, School of Medicine, Cancer Research Institute, 94143 0128, USA
    Nature 398:422-6. 1999
    ..Abnormal levels of beta-catenin may therefore contribute to neoplastic transformation by causing accumulation of cyclin D1...
  4. ncbi Entrainment of the circadian clock in the liver by feeding
    K A Stokkan
    National Science Foundation Center for Biological Timing and Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Science 291:490-3. 2001
    ..They also raise the possibility that peripheral circadian oscillators like those in the liver may be coupled to the SCN primarily through rhythmic behavior, such as feeding...
  5. ncbi Multiple microRNAs modulate p21Cip1/Waf1 expression by directly targeting its 3' untranslated region
    S Wu
    Department of Hematology, Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fuzhou, China
    Oncogene 29:2302-8. 2010
    ....
  6. ncbi Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo
    Bakhos A Tannous
    Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Ther 11:435-43. 2005
    ..and naturally secreted, with flash bioluminescence characteristics similar to those of other coelenterazine luciferases. hGLuc generated over 1000-fold higher bioluminescent signal intensity from live cells together with their ..
  7. pmc Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
    Angie M Cheng
    Ambion, Inc 2130 Woodward Street, Austin, TX 78744 1832, USA
    Nucleic Acids Res 33:1290-7. 2005
    ..From these screens, we conclude that miRNA-mediated regulation has a complexity of cellular outcomes and that miRNAs can be mediators of regulation of cell growth and apoptosis pathways...
  8. pmc Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    Rui Yi
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Genes Dev 17:3011-6. 2003
    ..Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function...
  9. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
    ..These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target...
  10. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  11. ncbi MicroRNA-125b suppresses the development of bladder cancer by targeting E2F3
    Li Huang
    Department of Urology, Sun Yat Sen Memorial Hospital, Sun Yat Sen University, Guangzhou, China
    Int J Cancer 128:1758-69. 2011
    ..Taken together, miR-125b may act as a tumor suppressor in bladder urothelium, and downregulation of miR-125b may contribute to the tumorigenesis of bladder cancer...
  12. pmc MicroRNA genes are transcribed by RNA polymerase II
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul, Korea
    EMBO J 23:4051-60. 2004
    ..These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes...
  13. pmc Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo
    Bakhos A Tannous
    Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Protoc 4:582-91. 2009
    ..Additionally, the Gluc signal can be detected in urine and the signal can be localized in animals using in vivo bioluminescence imaging...
  14. ncbi IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector
    H Mizuguchi
    Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan
    Mol Ther 1:376-82. 2000
    ..e., care should be taken regarding the decreased capacity of IRES-dependent downstream gene expression as well as in determining which gene should be positioned as the first or second gene in a bicistronic vector...
  15. ncbi Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
    Akin Akinc
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Gene Med 7:657-63. 2005
    ..According to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosomes, resulting in the release of the vector into the cytoplasm...
  16. pmc Bioluminescence imaging in mouse models quantifies beta cell mass in the pancreas and after islet transplantation
    John Virostko
    Vanderbilt University, Institute of Imaging Science, Nashville, TN, USA
    Mol Imaging Biol 12:42-53. 2010
    ..We developed a mouse model that enables non-invasive assessment of changes in beta cell mass...
  17. ncbi Advances in in vivo bioluminescence imaging of gene expression
    Christopher H Contag
    Department of Pediatrics, Stanford University School of Medicine, California 94305 5208, USA
    Annu Rev Biomed Eng 4:235-60. 2002
    ..technology for in vivo analysis employs internal biological sources of light emitted from luminescent enzymes, luciferases, to label genes and cells...
  18. pmc Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestration
    Blandine Franke-Fayard
    Department of Parasitology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 102:11468-73. 2005
    ..Real-time in vivo imaging of parasitic processes may be used to evaluate the molecular basis of pathology and develop strategies to prevent pathology...
  19. pmc Non-invasive detection of a small number of bioluminescent cancer cells in vivo
    Jae Beom Kim
    Caliper Life Sciences Inc, Alameda, California, United States of America
    PLoS ONE 5:e9364. 2010
    ..Significantly higher luciferase activity in these cells allowed us to detect micrometastases in both, syngeneic Balb/c and nu/nu mice...
  20. ncbi High-throughput mapping of a dynamic signaling network in mammalian cells
    Miriam Barrios-Rodiles
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5
    Science 307:1621-5. 2005
    ..We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions...
  21. pmc Use of the ODD-luciferase transgene for the non-invasive imaging of spontaneous tumors in mice
    Scott J Goldman
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America
    PLoS ONE 6:e18269. 2011
    ..Since solid tumors often contain hypoxic regions, we performed proof-of-principle experiments testing whether this transgenic mouse model may be used as a universal platform for non-invasive imaging analysis of spontaneous solid tumors...
  22. pmc Primary involvement of pharynx and peyer's patch in inhalational and intestinal anthrax
    Ian J Glomski
    Unité des Toxines et Pathogénie Bactérienne, Institut Pasteur, Paris, France
    PLoS Pathog 3:e76. 2007
    ..anthracis pathogenesis...
  23. pmc ATP-dependent human RISC assembly pathways
    Mayuko Yoda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Nat Struct Mol Biol 17:17-23. 2010
    ..All these features of human AGO proteins are highly reminiscent of fly Ago1 but not fly Ago2...
  24. pmc Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract
    M Chelsea Lane
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    Proc Natl Acad Sci U S A 104:16669-74. 2007
    ..These data provide compelling evidence that wild-type UPEC express flagellin and presumably utilize flagellum-mediated motility during UTI to ascend to the upper urinary tract and disseminate within the host...
  25. pmc Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes
    K Deitsch
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 29:850-3. 2001
    ..The findings reported here suggest that the malaria genome may be continually exposed to exogenous DNA from residual nuclear material in host erythrocytes...
  26. ncbi Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization
    Monique Verhaegent
    Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada
    Anal Chem 74:4378-85. 2002
    ..of GLuc with streptavidin (SA), thereby avoiding chemical conjugation reactions that are known to inactivate luciferases. Purified GLuc can be detected down to 1 amol with a signal-to-background ratio of 2 and a linear range ..
  27. ncbi The VNTR polymorphism of the human dopamine transporter (DAT1) gene affects gene expression
    S Fuke
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
    Pharmacogenomics J 1:152-6. 2001
    ..This suggests that VNTR polymorphism affects the expression of the dopamine transporter...
  28. pmc RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation
    Philippe P Roux
    Department of Pathology and Cell Biology, Institut de recherche en immunologie et en cancérologie IRIC, Universite de Montreal, Montreal, Quebec, Canada
    J Biol Chem 282:14056-64. 2007
    ..These data demonstrate that RSK provides an mTOR-independent pathway linking the Ras/ERK signaling cascade to the translational machinery...
  29. pmc Optimisation of bioluminescent reporters for use with mycobacteria
    Nuria Andreu
    Department of Medicine, Imperial College London, London, UK
    PLoS ONE 5:e10777. 2010
    ..Despite the extensive use of luminescent reporters in mycobacteria, the resultant luminescent strains have not been fully applied to BLI...
  30. ncbi Applications of bioluminescence imaging to the study of infectious diseases
    Martha Hutchens
    Immunology Program, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    Cell Microbiol 9:2315-22. 2007
    ..We expect that both the range of organisms and the molecular events able to be studied by BLI will continue to expand, yielding important insights into mechanisms of pathogenesis...
  31. ncbi Resetting central and peripheral circadian oscillators in transgenic rats
    S Yamazaki
    NSF Center for Biological Timing and Department of Biology, University of Virginia, Charlottesville, VA 22903-2477, USA
    Science 288:682-5. 2000
    ....
  32. pmc Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals
    Kathryn E Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:12288-93. 2004
    ....
  33. ncbi Circadian clock mutants in Arabidopsis identified by luciferase imaging
    A J Millar
    National Science Foundation NSF Center for Biological Timing, Department of Biology, University of Virginia, Charlottesville 22903
    Science 267:1161-3. 1995
    ....
  34. ncbi Self-illuminating quantum dot conjugates for in vivo imaging
    Min Kyung So
    Molecular Imaging Program, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305 5484, USA
    Nat Biotechnol 24:339-43. 2006
    ..Compared with existing quantum dots, self-illuminating quantum dot conjugates have greatly enhanced sensitivity in small animal imaging, with an in vivo signal-to-background ratio of > 10(3) for 5 pmol of conjugate...
  35. ncbi GATA4 loss-of-function mutations in familial atrial fibrillation
    Yi qing Yang
    Department of Cardiovascular Research, Shanghai Chest Hospital, Medical College of Shanghai Jiaotong University, PR China
    Clin Chim Acta 412:1825-30. 2011
    ..Growing studies demonstrate that genetic defects play pivotal roles in a subgroup of AF. However, AF is a genetically heterogeneous disorder and the molecular basis of AF in a majority of cases remains unknown...
  36. ncbi In vivo imaging of light-emitting probes
    B W Rice
    Xenogen Corporation, 860 Atlantic Avenue, Alameda, CA 94501, USA
    J Biomed Opt 6:432-40. 2001
    ..Instrumentation for in vivo imaging developed at Xenogen is described and several examples of images of mice with bioluminescent cells are presented...
  37. ncbi Superluminescent variants of marine luciferases for bioassays
    Sung Bae Kim
    Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology AIST, Onogawa, Tsukuba, Japan
    Anal Chem 83:8732-40. 2011
    In this study, a rational synthesis of superluminescent variants from marine luciferases with prolonged bioluminescence has been demonstrated...
  38. pmc High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases
    Naoki Hida
    Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    PLoS ONE 4:e5868. 2009
    ..We herein describe an imaging method for real-time analysis of protein-protein interactions using multicolor luciferases with different spectral characteristics...
  39. ncbi Biophysical characterization of highly active recombinant Gaussia luciferase expressed in Escherichia coli
    Tharangani Rathnayaka
    Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2 24 16 Nakamachi, Koganei Shi, Tokyo 184 8588, Japan
    Biochim Biophys Acta 1804:1902-7. 2010
    ..These results indicate that both the structure and bioluminescence activity of GLuc remain stable at high temperatures, and they strongly suggest GLuc's potential as a reporter protein...
  40. pmc siRNAs can function as miRNAs
    John G Doench
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 17:438-42. 2003
    ..The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs...
  41. pmc Assessment of immunity to mycobacterial infection with luciferase reporter constructs
    V A Snewin
    Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St Mary s Campus, London W2 1PG, United Kingdom
    Infect Immun 67:4586-93. 1999
    ....
  42. ncbi Cell uptake and tissue distribution of radioiodine labelled D-luciferin: implications for luciferase based gene imaging
    K H Lee
    Department of Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
    Nucl Med Commun 24:1003-9. 2003
    ..Thus, substrate availability should be considered as a potential limiting factor for photon emission efficiency in certain organs when attempting quantitative interpretation of optical luc gene imaging...
  43. ncbi Calcineurin/NFAT coupling participates in pathological, but not physiological, cardiac hypertrophy
    Benjamin J Wilkins
    Division of Molecular Cardiovascular Biology, Department of Pediatrics, Children s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, Ohio 45229 3039, USA
    Circ Res 94:110-8. 2004
    ....
  44. pmc Real-time imaging of the somite segmentation clock: revelation of unstable oscillators in the individual presomitic mesoderm cells
    Yoshito Masamizu
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 103:1313-8. 2006
    ....
  45. ncbi Induction of IL-8 expression by human herpesvirus 8 encoded vFLIP K13 via NF-kappaB activation
    Q Sun
    Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center, Dallas, TX, USA
    Oncogene 25:2717-26. 2006
    ....
  46. ncbi Non-invasive imaging of GFAP expression after neuronal damage in mice
    Lingyun Zhu
    Xenogen Corporation, 860 Atlantic Avenue, Alameda, CA 94501, USA
    Neurosci Lett 367:210-2. 2004
    ....
  47. ncbi Simple and sensitive antimalarial drug screening in vitro and in vivo using transgenic luciferase expressing Plasmodium berghei parasites
    B Franke-Fayard
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
    Int J Parasitol 38:1651-62. 2008
    ..The standard methodology of anti-malarial drug screening and validation, which includes testing in rodent models of malaria, can be improved by the incorporation of such assays...
  48. ncbi Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasis
    Darlene E Jenkins
    Xenogen Corporation, Alameda, California 94501, USA
    Clin Exp Metastasis 20:733-44. 2003
    ..The ability to temporally assess tumor development and responses to drug therapies in vivo also improves upon current standard animal models that are based on single end point data...
  49. pmc Dual-color click beetle luciferase heteroprotein fragment complementation assays
    Victor Villalobos
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Department of Developmental Biology, Washington University, St Louis, MO 63110, USA
    Chem Biol 17:1018-29. 2010
    ..of reversible multicolored heteroprotein complementation fragments based on various firefly and click beetle luciferases that utilize the same substrate, D-luciferin...
  50. ncbi In vivo bioluminescence imaging for integrated studies of infection
    Timothy C Doyle
    Molecular Imaging Program at Stanford MIPS, Clark Center, Bio X Program, 318 Campus Drive, Room E 150, Stanford University School of Medicine, Stanford, CA 94305 5427, USA
    Cell Microbiol 6:303-17. 2004
    ..The use of internal biological sources of light, luciferases, to tag cells, pathogens, and genes has proved to be a versatile tool to provide in vivo indicators that can be ..
  51. ncbi Rational siRNA design for RNA interference
    Angela Reynolds
    Dharmacon, Inc, 2650 Crescent Drive, Suite 100, Lafayette, Colorado 80026, USA
    Nat Biotechnol 22:326-30. 2004
    ..This highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies...
  52. ncbi A highly sensitive protein-protein interaction assay based on Gaussia luciferase
    Ingrid Remy
    Departement de Biochimie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, Quebec, H3C 3J7, Canada
    Nat Methods 3:977-9. 2006
    ..We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling...
  53. pmc Noninvasive bioluminescence imaging of herpes simplex virus type 1 infection and therapy in living mice
    Gary D Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 76:12149-61. 2002
    ..These data demonstrate that bioluminescence imaging can be used for noninvasive, real-time monitoring of HSV-1 infection and therapy in living mice...
  54. ncbi Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells
    Jolene M Ignowski
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, MC 1462, University of California Berkeley, Berkeley, CA 94720 1462, USA
    Biotechnol Bioeng 86:827-34. 2004
    ..Finally, we have modeled luciferase activity in live cells for the purposes of understanding and translating the luciferase signal into a more effective metric of gene expression and cell behavior...
  55. ncbi Bioluminescent imaging: a critical tool in pre-clinical oncology research
    Karen O'Neill
    UCD School of Medicine and Medical Science, Health Science Building, University College Dublin, Belfield, Dublin 4, Ireland
    J Pathol 220:317-27. 2010
    ..Such novel studies highlight the versatility and potential of bioluminescent imaging in future oncological research...
  56. pmc Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions
    Simon D'Archivio
    Laboratoire des Processus Infectieux à Trypanosoma, Department of Infection and Epidemiology, Paris, France
    PLoS Negl Trop Dis 5:e1461. 2011
    ..We then developed highly reproducible conditions that can be used to obtain and select stably transfected mutants that continue metacyclogenesis and are infectious in immunocompetent rodents...
  57. pmc Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro
    Gregory Michel
    Universite de Nice Sophia Antipolis, Faculte de Medecine, Nice, France
    PLoS Negl Trop Dis 5:e1323. 2011
    ..This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection...
  58. pmc Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system
    Bram Stynen
    Department of Molecular Microbiology, VIB, Leuven, Belgium
    Microbiol Mol Biol Rev 76:331-82. 2012
    ..Applications of these technologies are discussed, together with the advantages and limitations of the available assays...
  59. ncbi Noninvasive monitoring of β-cell mass and fetal β-cell genesis in mice using bioluminescence imaging
    Yukari Sekiguchi
    Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8575, Japan
    Exp Anim 61:445-51. 2012
    ..In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri...
  60. pmc Evaluation of immunity against malaria using luciferase-expressing Plasmodium berghei parasites
    Ivo Ploemen
    Department of Medical Microbiology, Radboud University Nijmegen Medical Center RUNMC, Nijmegen, The Netherlands
    Malar J 10:350. 2011
    ..This study explores the benefit of using these parasites for the evaluation of immunity against malaria, compared to qRT-PCR techniques in vivo and in vitro...
  61. pmc Profile of the GSK published protein kinase inhibitor set across ATP-dependent and-independent luciferases: implications for reporter-gene assays
    Patricia Dranchak
    National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States of America
    PLoS ONE 8:e57888. 2013
    ..The study also underscores the general need for strategies such as the use of orthogonal reporters to identify kinase or non-kinase mediated cellular responses...
  62. pmc Cardiac atrial circadian rhythms in PERIOD2::LUCIFERASE and per1:luc mice: amplitude and phase responses to glucocorticoid signaling and medium treatment
    Daan R van der Veen
    Department of Biological Sciences, University of Notre Dame, Indiana, United States of America
    PLoS ONE 7:e47692. 2012
    ..The current data describe novel entrainment signals for the atrial circadian clock and specifically highlight entrainment by mechanical treatment, an intriguing observation considering the mechanical nature of cardiac tissue...
  63. pmc A highly sensitive assay for monitoring the secretory pathway and ER stress
    Christian E Badr
    Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 2:e571. 2007
    ..We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc)...
  64. pmc Activation of the nucleotide oligomerization domain signaling pathway by the non-bacterially derived xanthone drug 5'6-dimethylxanthenone-4-acetic acid (Vadimezan)
    Guanjun Cheng
    Thoracic Oncology Research Laboratory, University of Pennsylvania, 3615 Civic Center Boulevard, Philadelphia, Pennsylvania 19104 4318, USA
    J Biol Chem 285:10553-62. 2010
    ..This information also expands our knowledge on the mechanisms of action of the anti-tumor agent DMXAA (currently in clinical trials) and may be important for its biological activity...
  65. ncbi Evaluation of impaired beta-cell function in nonobese-diabetic (NOD) mouse model using bioluminescence imaging
    Dror Sever
    Department of Endocrinology, Hadassah University Hospital, P O Box 12000, 91120 Jerusalem, Israel
    FASEB J 25:676-84. 2011
    ....
  66. ncbi Noninvasive real-time monitoring of liver-stage development of bioluminescent Plasmodium parasites
    Agnes Mwakingwe
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Infect Dis 200:1470-8. 2009
    ..Plasmodium liver stages are the targets of new chemotherapeutics and vaccines, but there are limited tools available to study this stage in vivo...
  67. ncbi A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells
    S Freundlieb
    Zentrum für Molekulare Biologie ZMBH, Universitat Heidelberg, Germany
    J Gene Med 1:4-12. 1999
    ..g., during transient expression, intrinsic residual transcription persists in their 'off' state, which can differ in intensity among different cell types...
  68. ncbi Non-viral S/MAR vectors replicate episomally in vivo when provided with a selective advantage
    S P Wong
    Gene Therapy Research Group, Section of Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, UK
    Gene Ther 18:82-7. 2011
    ..These results provide proof-of-principle that S/MAR vectors are capable of preventing transgene silencing, are resistant to integration and are able to confer mitotic stability in vivo when provided with a selective advantage...
  69. pmc Transglutaminase 2 regulates early chondrogenesis and glycosaminoglycan synthesis
    Dmitry Nurminsky
    Dept Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
    Mech Dev 128:234-45. 2011
    ..These findings further advance understanding of cartilage formation and disease, and contribute to cartilage bioengineering...
  70. pmc In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodies
    Nicholas S Heaton
    Icahn School of Medicine at Mount Sinai, Department of Microbiology, New York, New York, USA
    J Virol 87:8272-81. 2013
    ..The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future antiviral therapies. ..
  71. ncbi In vivo imaging of functional targeting of miR-221 in papillary thyroid carcinoma
    Hyun Joo Kim
    Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea
    J Nucl Med 49:1686-93. 2008
    ..To evaluate the abundant gene regulation of miR-221 in papillary thyroid carcinoma (PTC), we performed microarray analysis and developed a Gaussia luciferase (Gluc) reporter system regulated by miR-221...
  72. pmc miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes
    Qin Liu
    Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People s Republic of China
    Nucleic Acids Res 36:5391-404. 2008
    ..Taken together, our data suggests that miR-16 family triggers an accumulation of cells in G0/G1 by silencing multiple cell cycle genes simultaneously, rather than the individual target...
  73. ncbi The orphan nuclear receptor Nur77 regulates decidual prolactin expression in human endometrial stromal cells
    Yue Jiang
    Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, PR China
    Biochem Biophys Res Commun 404:628-33. 2011
    ..01) induced by 8-Br-cAMP and MPA. These results demonstrate that Nur77 is a novel transcription factor that contributes significantly to the regulation of prolactin gene expression in human endometrial stromal cells...
  74. ncbi Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Prefecture 278 0037, Japan
    Biochim Biophys Acta 1597:271-9. 2002
    ..cruciata luciferase. The pH-dependence of the bioluminescence emission spectra was also examined. In contrast to wild-type firefly luciferases characterized to date, the mutant did not show the pH-dependent red spectrum shift.
  75. pmc Dynamic changes in the microRNA expression profile reveal multiple regulatory mechanisms in the spinal nerve ligation model of neuropathic pain
    David von Schack
    Biological Technologies, Pfizer Global Research and Development, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e17670. 2011
    ..Our results suggest the potential for miRNAs to play a direct role in neuropathic pain...
  76. ncbi The role of cell types in cytomegalovirus infection in vivo
    Torsten Sacher
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    Eur J Cell Biol 91:70-7. 2012
    ..This allowed us to study the role of endothelial cells and hepatocytes for virus dissemination and will permit detailed studies on innate and adaptive immune responses to CMV...
  77. ncbi Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging
    Katy M Venisnik
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Mol Imaging Biol 9:267-77. 2007
    ..4% ID/g) at 21 hours. Although further optimization of this fusion protein may be needed to improve in vivo performance, the diabody-GLDelta15 is a promising optical imaging probe for tumor detection in vivo...
  78. ncbi A sensitive phenotypic assay for the determination of human immunodeficiency virus type 1 tropism
    Nuria González
    AIDS Immunopathology Unit, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    J Antimicrob Chemother 65:2493-501. 2010
    ..To develop a sensitive phenotypic assay based on recombinant viruses (RVs) for characterizing HIV-1 tropism...
  79. pmc Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging
    Bradley A Creamer
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Genesis 47:234-45. 2009
    ..The study shows that, unlike their progeny in mammary epithelial transplants as reported previously, PI-MECs themselves may not belong to the long-term label-retaining epithelial subtype...
  80. pmc Structural basis for the inhibition of firefly luciferase by a general anesthetic
    N P Franks
    Biophysics Section, The Blackett Laboratory, Imperial College of Science, Technology and Medicine, London, England
    Biophys J 75:2205-11. 1998
    ..2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity...
  81. ncbi Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing
    T Ozawa
    Department of Chemistry, School of Science, The University of Tokyo, Japan
    Anal Chem 73:2516-21. 2001
    ..High-throughput drug screening and quantitative analysis for a specific pathway in tyrosine phosphorylation of IRS-1 in insulin signaling are also made possible in this system...
  82. ncbi An improved tet-on system for gene expression in neurons delivered by a single lentiviral vector
    Xuebi Tian
    Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Hum Gene Ther 20:113-23. 2009
    ..Transgene expression from the NSE promoter also provided tightly regulated gene expression in neurons in vivo...
  83. pmc Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice
    Irene Bergwerf
    Laboratory of Experimental Hematology, University of Antwerp, Antwerp, Belgium
    BMC Biotechnol 9:1. 2009
    ..However, in many pre-clinical cell therapy studies, reporter gene-assisted imaging of cellular implants in the CNS and potential reporter gene and/or cell-based immunogenicity, still remain challenging research topics...
  84. pmc Protective role of reactive oxygen species in endotoxin-induced lung inflammation through modulation of IL-10 expression
    Jing Deng
    Section of Pulmonary, Critical Care, Sleep, and Allergy, Department of Medicine, University of Illinois, Chicago, IL 60612, USA
    J Immunol 188:5734-40. 2012
    ..Our data support the innovative concept that generation of ROS is essential for counterregulation of acute lung inflammation...
  85. ncbi Novel GATA4 mutations in lone atrial fibrillation
    Jin qi Jiang
    Department of Emergency, Shanghai Chest Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200030, PR China
    Int J Mol Med 28:1025-32. 2011
    ..The findings expand the mutation spectrum of GATA4 linked to AF and provide novel insight into the molecular mechanism involved in the pathogenesis of AF...
  86. ncbi High-active truncated luciferase of copepod Metridia longa
    Svetlana V Markova
    Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia
    Biochem Biophys Res Commun 417:98-103. 2012
    ..The results clearly suggest that the truncated Metridia luciferase is well suited as a secreted reporter ensuring higher detection sensitivity in comparison with a wild type enzyme...
  87. ncbi Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring
    Mi Sook Lee
    Division of Bio Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, Kangwon Do 200 701, Republic of Korea
    Biochem Biophys Res Commun 394:348-53. 2010
    ..This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy...
  88. ncbi Clinical potential of intravenous neural stem cell delivery for treatment of neuroinflammatory disease in mice?
    Kristien P Reekmans
    Laboratory of Experimental Hematology, University of Antwerp, Belgium
    Cell Transplant 20:851-69. 2011
    ..However, given the fact that grafted NSCs have an intrinsic survival capacity in the CNS, their therapeutic exploitation should be further investigated, and-in contrast to several other reports-will most likely be highly complex...
  89. pmc AAV-mediated in vivo knockdown of luciferase using combinatorial RNAi and U1i
    A Koornneef
    Department of Research and Development, Amsterdam Molecular Therapeutics, Amsterdam, The Netherlands
    Gene Ther 18:929-35. 2011
    ..In conclusion, these results provide a proof of principle for the combinatorial use of RNAi and U1i to enhance target gene knockdown in vivo...
  90. pmc Enhanced beetle luciferase for high-resolution bioluminescence imaging
    Yoshihiro Nakajima
    National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan
    PLoS ONE 5:e10011. 2010
    ..These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc...
  91. pmc In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals
    Dan M Close
    The Center for Environmental Biotechnology, 676 Dabney Hall, The University of Tennessee, Knoxville, TN 37996, USA
    Sensors (Basel) 11:180-206. 2011
    ..This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism...
  92. pmc Shifting foci of hematopoiesis during reconstitution from single stem cells
    Yu An Cao
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:221-6. 2004
    ....
  93. pmc Inhibition of intracellular hepatitis C virus replication by synthetic and vector-derived small interfering RNAs
    Takanori Yokota
    Department of Neurology and Neurological Science, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    EMBO Rep 4:602-8. 2003
    ..Our results support the feasibility of using siRNA-based gene therapy to inhibit HCV replication, which may prove to be valuable in the treatment of hepatitis C...
  94. ncbi Visualizing gene expression in living mammals using a bioluminescent reporter
    C H Contag
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5119, USA
    Photochem Photobiol 66:523-31. 1997
    ..Thus, real-time, noninvasive study of gene expression in living animal models for human development and disease is possible...
  95. pmc Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase
    Laura Mezzanotte
    Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy
    PLoS ONE 6:e19277. 2011
    ..A new red emitting codon-optimized luciferase reporter gene mutant of Photinus pyralis, Ppy RE8, has been developed and used in combination with the green click beetle luciferase, CBG99...
  96. pmc Luciferase imaging of a neurotropic viral infection in intact animals
    Susan H Cook
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Virol 77:5333-8. 2003
    ....
  97. ncbi Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes
    Takako Noguchi
    National Institute of Advanced Industrial Science and Technology AIST, 1 8 31 Midorigaoka, Ikeda, Osaka 563 8577, Japan
    Biochemistry 49:8053-61. 2010
    ..The expression of the two genes is monitored with green- and red-emitting beetle luciferases with a single luminescent substrate...
  98. pmc Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line
    Dan M Close
    The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee, United States of America
    PLoS ONE 5:e12441. 2010
    ....
  99. ncbi In vitro gene delivery mediated by chitosan. effect of pH, serum, and molecular mass of chitosan on the transfection efficiency
    T Sato
    Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan
    Biomaterials 22:2075-80. 2001
    ..Heptamer (1.3 kDa) did not show any gene expression. Cationic liposome (lipofectin)-associated gene expression was inhibited by serum, while chitosan showed resistance to serum...
  100. ncbi Guided by the light: visualizing biomolecular processes in living animals with bioluminescence
    Jennifer A Prescher
    Molecular Imaging Program at Stanford, Stanford School of Medicine, Stanford, CA, USA
    Curr Opin Chem Biol 14:80-9. 2010
    ..b>Luciferases can be incorporated into a variety of non-luminescent hosts and used to track cells, visualize gene expression, ..
  101. ncbi Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice
    Blandine Franke-Fayard
    Malaria Research Group, Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Nat Protoc 1:476-85. 2006
    ..Real-time in vivo imaging of infected erythrocytes will provide increased insights into the dynamics of sequestration and its role in pathology, and can be used to evaluate strategies that prevent sequestration...

Research Grants75

  1. Myxoma Virus (MV) Oncolysis for treating human cancer
    Grant McFadden; Fiscal Year: 2013
    ..The cancer cells and viruses will be tagged with distinguishable luciferases that allow the engrafted tumor cells and the therapeutic virus to be independently tracked in vivo...
  2. Diagnostic assay systems for Botulinum Neurotoxins
    Markus Kalkum; Fiscal Year: 2013
    ..Bioluminescent substrates will be produced as genetically- engineered inactive versions of luciferases that are activated by BoNTs in the microcolumn-based ALISSA...
  3. Genetically Encoded Light-Production and Light-Sensing for Neuronal Manipulation
    UTE H HOCHGESCHWENDER; Fiscal Year: 2013
    ..e. combining light-sensing molecules (opsins) with biologically produced light through luciferases. The concept of having light-production and light-sensing each genetically encoded permits a) to expand the use ..
  4. An Autonomous Biologic Controller for Optogenetic Applications
    Jack Tung; Fiscal Year: 2013
    ..In the first aim, we will show that luciferases can be used to activate opsins in vitro...
  5. Expanding the bioluminescent toolbox for multi-cellular imaging of tumor heteroge
    JENNIFER PRESCHER; Fiscal Year: 2013
    ..This technology employs enzymes (luciferases) that produce light upon incubation with small molecule substrates (luciferins)...
  6. Autonomous Optogenetic Inhibition of Epileptic Activity Using a Bioluminescent Li
    Robert E Gross; Fiscal Year: 2013
    ..Calcium-sensitive luciferases are a kind of bioluminescent reporter that has been successfully used for imaging neural activity in vivo...
  7. Luciferase-encoded influenza viruses for antiviral screening
    DEAN KEDES; Fiscal Year: 2006
    ..which was designed to allow robotic high-throughput analysis of cells containing genes of Firefly and Renilla luciferases grown in 96- or 384-well plates...
  8. Enhanced synthetic luciferin substrates for firefly luciferase
    Stephen C Miller; Fiscal Year: 2013
    ..1) synthesis of new luciferin substrates with enhanced light emission properties;2) identification of mutant luciferases that efficiently utilize synthetic luciferins;3) evaluation of synthetic luciferins and mutant luciferases for ..
  9. Xenogen IVIS Imaging System 200
    LAURA KNOLL; Fiscal Year: 2007
    ..a living animal when D-Luciferin or Coelenterazine is injected and used as a substrate by firefly or renilla luciferases, respectively...
  10. Understanding and finding new fluorescent proteins
    Marc Zimmer; Fiscal Year: 2009
    ..Proteins in the Global Ocean Sampling Expedition Database Bioinformatic and homology modeling of FPs and luciferases in GOS. 5...
  11. NOVEL BEETLE LUCIFERASES FOR BIOMEDICAL DEVELOPMENT
    Keith Wood; Fiscal Year: 1993
    ..Through fundamental research on the enzymatic mechanism of beetle luciferases, Promega Corporation has developed new chemistry which provides greater luminescence intensity with simpler ..
  12. Cellular mechanisms of ketogenic diet epilepsy treatment
    JAMES BERG; Fiscal Year: 2006
    ..isolated neurons while changing their metabolic state with ketone bodies, then by using an innovative optical technique, targeted luciferases, to investigate possible fluctuations in sub-cellular Iocalizations of ATP.
  13. VISIBLE ANIMAL MODELS OF NEOPLASTIC DISEASE
    Christopher Contag; Fiscal Year: 2002
    ..cell sorter (FACS) by developing multifunctional reporter genes (fusion proteins comprised of luciferases and fluorescent proteins) that are detectable in vivo by bioluminescence and ex vivo by fluorescence...
  14. Extremely Stable and Bright Periphylla Luciferase
    MILLARD CULL; Fiscal Year: 2005
    b>Luciferases have become invaluable detection tools in molecular biology and other fields. Their utility is imited by their physical liability...
  15. PRODUCTION AND FUNCTION OF GHRH IN THE IMMUNE SYSTEM
    DOUGLAS WEIGENT; Fiscal Year: 2003
    ..It is further anticipated that the new understanding gained from the results will identify important sites for drug intervention against various immunodeficiencies including AIDS, autoimmunity, and aging. ..
  16. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
    ..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. [unreadable] [unreadable] [unreadable]..
  17. Glycosaminoglycan-based inhibitors for treatment of prostate cancer
    Vinata B Lokeshwar; Fiscal Year: 2010
    ..If proven efficacious, sHA compounds and 4-MU will be a novel class of glycosaminoglycan-based target-specific anti-tumor drugs. ..
  18. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  19. Nutritional manipulation of circadian rhythms and cancer
    Alec Davidson; Fiscal Year: 2007
    ..The experiments will form the basis for future study of circadian and nutritional influences on development of this and other cancers. [unreadable] [unreadable] [unreadable]..
  20. PSMA-based PET Ligands for Prostate Cancer Imaging
    Martin Pomper; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. Anticarcinogenic Mechanisms of Tea Constituents
    Zigang Dong; Fiscal Year: 2007
    ....
  22. Regulation of Sulfotransferases by LXR and its Implication in Pathophysiology
    Wen Xie; Fiscal Year: 2011
    ..Since estrogens are prerequisites for breast cancer, delineating the regulatory effects of LXRs on EST/SULT1E1 expression may have a broader significance in the understanding of breast cancer prevention and treatment. ..
  23. The Hepatoprotective Role of the Orphan Nuclear Receptor LXR
    Wen Xie; Fiscal Year: 2010
    ..It is hoped that the development of selective LXR agonists may represent a novel strategy for the prevention and treatment of APAP-induced hepatotoxicity. ..
  24. Study on Ultraviolet-induced Signal Transduction
    Zigang Dong; Fiscal Year: 2010
    ..These preclinical studies are designed to lead to the development of new strategies for the chemoprevention of human skin cancers. ..
  25. PXR Controlled Bile Acid Detoxification in Colon Cancer
    Wen Xie; Fiscal Year: 2008
    ..It is anticipated that the development of selective PXR agonists may represent a novel strategy to eliminate toxic bile acids and to prevent colon cancer. ..
  26. Electroporation System for Cutaneous Gene Transfer
    Richard Heller; Fiscal Year: 2009
    ..This information will help establish an effective way to deliver naked DNA to the skin. The investigators have pioneered the use of electroporation for in vivo delivery so are well suited to successfully complete the study. ..
  27. Mechanisms of chemopreventive effect of resveratrol
    Zigang Dong; Fiscal Year: 2009
    ..Our long-term goal is to identify the molecular mechanism explaining the anti-tumor effect exhibited by resveratrol. Such knowledge will help in developing better chemopreventive agents with fewer side effects. ..
  28. INHIBITION OF CARCINOGENESIS BY TEA AND TEA CONSTITUENTS
    Zigang Dong; Fiscal Year: 2010
    ..abstract_text> ..
  29. High density mutagenesis of mtDNA
    Mikhail Alexeyev; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  30. Anti-HIV-1 Composite Cellulose Acetate Phthalate Film
    Shibo Jiang; Fiscal Year: 2008
    ....
  31. PSMA-based Gene Reporter-Probe System(RMI)
    Martin Pomper; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  32. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  33. Mucosal Helper-dependent Adenovirus Vaccines Against HIV-1
    Michael Barry; Fiscal Year: 2008
    ..Successful pursuit of this project will enable more specific and less dangerous adenoviral vaccines for future testing in rhesus macaque SHIV challenge models and for future use in humans. ..
  34. Linking folate deficiency to changes in gene expression
    KAREN KATULA; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  35. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  36. DNA damage signaling in oxygen toxicity in lung cells
    KUMUDA DAS; Fiscal Year: 2007
    ..Using these molecular approaches, we will define the mechanisms of DNA damage signaling n hyperoxia, which will significantly improve our understanding of pulmonary oxygen toxicity in lung cells [unreadable] [unreadable]..
  37. PROSTATIC VASCULOGENIC MIMICRY: A NEW METASTATIC PATHWAY
    Mary Hendrix; Fiscal Year: 2003
    ..abstract_text> ..
  38. Gene Therapy, Neural Grafts & Parkinson's Disease
    DAVID YUREK; Fiscal Year: 2005
    ..These studies will also determine if combinations of viral vectors expressing different neurotrophic factors can be used to improve the therapeutic effects of dopamine grafts. ..
  39. Circadian organization in the aged rodent
    Alec Davidson; Fiscal Year: 2004
    ....
  40. GENETIC VACCINES AGAINST HIV MUTANTS
    Michael Barry; Fiscal Year: 2004
    ..If successful, this project in HLA-A 0201 MHC I transgenic mice will lay the groundwork for future prophylactic vaccine trials in rhesus macaques and ultimately in humans. ..
  41. PHENOTYPE SCREENS FOR BONE MARROW FAILURE
    Michael Barry; Fiscal Year: 2003
    ..End of Abstract.) ..
  42. Poly (ADP-Ribose) synthetase in expression of endothelia
    Basilia Zingarelli; Fiscal Year: 2003
    ..abstract_text> ..
  43. Mineralocorticoid-R Control of HPA Stress Response
    THADDEUS PACE; Fiscal Year: 2003
    ..And finally, the role of MR in controlling HPA responding will be examined after a stressor has been experienced repeatedly, a situation previously shown to increase the number of brain MR. ..
  44. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  45. IN VIVO REPORTER SYSTEM FOR IMAGING GENE TRANSFER
    Kurt Zinn; Fiscal Year: 2002
    ..The proposed reporter system will determine if gene therapy vectors are targeted to tumor, and when further gene therapy treatment is necessary. ..
  46. ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
    Richard Heller; Fiscal Year: 2002
    ..An important consideration in the treatment of soft tissue sarcoma is to be able to perform limb sparing procedures which maintain functionality. ECT combined with surgery could fill this role. ..
  47. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  48. CNS METABOLIC CORRELATES OF HIV DEMENTIA BY MRS IMAGING
    Martin Pomper; Fiscal Year: 2001
    ..abstract_text> ..
  49. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  50. Imaging Serotonergic Transmission in HIV Depression
    Martin Pomper; Fiscal Year: 2006
    ..abstract_text> ..
  51. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  52. Identification of new Wnt signaling components
    Kenneth Cadigan; Fiscal Year: 2006
    ..Obtaining genetic confirmation of the importance of these putative Wnt signaling components will provide the confidence to pursue their study in the context of cancer biology and as targets for drug discovery. ..
  53. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005
    ..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
  54. Nipah Virus and Hendra Virus Subunit Vaccines
    Christopher Broder; Fiscal Year: 2006
    ..We will evaluate monoclonal and polyclonal antibodies in both reporter-gene membrane fusion assays and in virus-neutralization assays. ..
  55. Cell Targeting Ligands and Vectors for CLL
    Michael Barry; Fiscal Year: 2005
    ..abstract_text> ..
  56. Glycosaminoglycans For IC Pathophysiology and Prognosis
    Vinata Lokeshwar; Fiscal Year: 2005
    ..Furthermore, it might yield a test or a combination of tests that can be used in the follow-up of IC patients and for monitoring 9 treatment responses. ..
  57. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. Stem Cell Adaptability in Parkinson' Disease
    DAVID YUREK; Fiscal Year: 2009
    ..The variable of host age on ESC-derived dopamine neuron adaptability will be studied. ..
  59. Nitric oxide mimetic NSAIDs for treatment of Alzheimer's Disease
    GREGORY THATCHER; Fiscal Year: 2007
    ..This project will yield a drug candidate for which amyloid load will be measured in transgenic models in subsequent research, providing the impetus for moving this drug candidate from discovery to the clinic. [unreadable] [unreadable]..
  60. Menopause: Decreased Response to Increasing Inflammation
    ADRIANA CATERINA MAGGI; Fiscal Year: 2010
    ....
  61. ALVEOLAR TYPE II CELL GROWTH IN INJURY
    RANDOLPH HASTINGS; Fiscal Year: 2003
    ..The project will evaluate the potential use of PTHrP-related therapeutic interventions that might speed or improve recovery following silica-induced lung injury. ..
  62. Variation in SP-C and Key UPR Genes among Infants with Respiratory Distress
    JENNIFER WAMBACH; Fiscal Year: 2008
    ..Finally, the third aim is to look at the interaction of SFTPC variants with genes of the UPR that increase the risk for RDS using sequential logistic regression. [unreadable] [unreadable] [unreadable] [unreadable]..
  63. Association and Function of Opioid Receptor Gene Variants to Substance Dependence
    Huiping Zhang; Fiscal Year: 2008
    ..Jeffrey Gruen (co-mentor), who are both excellent supervisors with substantial experience in genetic studies of complex disorders and who have both mentored numerous previous trainees. [unreadable] [unreadable] [unreadable]..
  64. Mouse Modeling Techniques for Bioluminescence Tomography
    Wenxiang Cong; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  65. Role of Integrins in Pulmonary Fibrosis
    Eric White; Fiscal Year: 2006
    ....
  66. Biochemical analysis of the human vs. mouse Ah receptor
    Gary Perdew; Fiscal Year: 2007
    ..Collectively, these studies will establish the level of divergence in human vs. mouse AhR function and should allow a better assessment of the significance of human exposure to TCDD. [unreadable] [unreadable] [unreadable]..
  67. Xenobiotic Receptors in toxicology and Carcinogenesis
    Gary Perdew; Fiscal Year: 2002
    ..A detailed web site has been established: http://www. bmb. psu. edu/symposium/. ..
  68. STRUCTURE/FUNCTION OF THE CCK-B/GASTRIN RECEPTOR
    Alan Kopin; Fiscal Year: 2004
    ....
  69. Molecular Analysis of Dopamine 2 Like Receptor Function
    Alan Kopin; Fiscal Year: 2009
    ..Once identified, these genes will be functionally assessed to determine their potential role in dopaminergic signaling. ..
  70. GPCR Variants as Genetic Determinants of Obesity
    Alan Kopin; Fiscal Year: 2009
    ..In Aim 3, combinations of anorexigenic, orexigenic and hedonic receptor variants will be assessed as potential synergistic or additive factors underlying the polygenic basis of obesity and/or other Look AHEAD study endpoints. ..
  71. Cloning of Ah receptor bound regulatory DNA
    Gary Perdew; Fiscal Year: 2005
    ..This method should in the future be utilized in biology for a wide range of mechanistic studies. ..
  72. Genetic Analysis of Feeding Behavior and Fat Deposition
    Alan Kopin; Fiscal Year: 2009
    ..Outliers will be characterized as described above. We anticipate that the proposed studies will identify novel genes relevant to the pathophysiology of human obesity. ..
  73. A Unified Reporter Gene for Multi-Modality Imaging
    Jianghong Rao; Fiscal Year: 2005
    ..abstract_text> ..