hydroxysteroid dehydrogenases

Summary

Summary: Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.

Top Publications

  1. ncbi Human and rodent type 1 11beta-hydroxysteroid dehydrogenases are 7beta-hydroxycholesterol dehydrogenases involved in oxysterol metabolism
    M Hult
    Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Cell Mol Life Sci 61:992-9. 2004
  2. ncbi Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli
    N Tanaka
    Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan
    Biochemistry 35:7715-30. 1996
  3. ncbi Minireview: 11beta-hydroxysteroid dehydrogenase type 1- a tissue-specific amplifier of glucocorticoid action
    J R Seckl
    Endocrinology Unit, Department of Medical Sciences, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Endocrinology 142:1371-6. 2001
  4. ncbi Glucocorticoids inhibit interconversion of 7-hydroxy and 7-oxo metabolites of dehydroepiandrosterone: a role for 11beta-hydroxysteroid dehydrogenases?
    Boaz Robinzon
    Department of Biochemistry, University of Louisville Health Science Center, Louisville, KY 40292, USA
    Arch Biochem Biophys 412:251-8. 2003
  5. ncbi Tissue-specific Cushing's syndrome, 11beta-hydroxysteroid dehydrogenases and the redefinition of corticosteroid hormone action
    Paul M Stewart
    Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, Birmingham B15 2TH, UK
    Eur J Endocrinol 149:163-8. 2003
  6. ncbi Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Chi Ching Hwang
    Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    J Biol Chem 280:3522-8. 2005
  7. pmc Transgenic amplification of glucocorticoid action in adipose tissue causes high blood pressure in mice
    Hiroaki Masuzaki
    Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 112:83-90. 2003
  8. ncbi Novel whole-cell biocatalysts with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid
    Michael Braun
    Institute of Biochemical Engineering, Technische Universitat Munchen, Boltzmannstr 15, 85748 Garching, Germany
    Appl Microbiol Biotechnol 95:1457-68. 2012
  9. ncbi Androgens, estrogens, and hydroxysteroid dehydrogenases
    Dario Mizrachi
    Divisions of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8857, USA
    Mol Cell Endocrinol 301:37-42. 2009
  10. ncbi Mechanism of proton transfer in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Yi Hsun Chang
    Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih Chuan 1st Road, Kaohsiung, Taiwan
    J Biol Chem 282:34306-14. 2007

Detail Information

Publications203 found, 100 shown here

  1. ncbi Human and rodent type 1 11beta-hydroxysteroid dehydrogenases are 7beta-hydroxycholesterol dehydrogenases involved in oxysterol metabolism
    M Hult
    Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    Cell Mol Life Sci 61:992-9. 2004
    ..These results demonstrate an enzymatic origin of species differences in 7-oxysterol metabolism, establish the origin of endogenous 7beta-OH cholesterol in humans, and point to a possible involvement of 11beta-HSD1 in atherosclerosis...
  2. ncbi Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli
    N Tanaka
    Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan
    Biochemistry 35:7715-30. 1996
    ....
  3. ncbi Minireview: 11beta-hydroxysteroid dehydrogenase type 1- a tissue-specific amplifier of glucocorticoid action
    J R Seckl
    Endocrinology Unit, Department of Medical Sciences, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Endocrinology 142:1371-6. 2001
    11beta-hydroxysteroid dehydrogenases (11beta-HSDs) catalyze the interconversion of active glucocorticoids (cortisol, corticosterone) and inert 11-keto forms (cortisone, 11-dehydrocorticosterone)...
  4. ncbi Glucocorticoids inhibit interconversion of 7-hydroxy and 7-oxo metabolites of dehydroepiandrosterone: a role for 11beta-hydroxysteroid dehydrogenases?
    Boaz Robinzon
    Department of Biochemistry, University of Louisville Health Science Center, Louisville, KY 40292, USA
    Arch Biochem Biophys 412:251-8. 2003
    ..Distinct species-specific routes of metabolism of DHEA and the interconversion of its metabolites obviate extrapolation of animal studies to humans...
  5. ncbi Tissue-specific Cushing's syndrome, 11beta-hydroxysteroid dehydrogenases and the redefinition of corticosteroid hormone action
    Paul M Stewart
    Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, Birmingham B15 2TH, UK
    Eur J Endocrinol 149:163-8. 2003
    ..Modulation of enzyme activity may offer a future therapeutic approach to treating these diseases whilst circumventing the endocrine consequences of glucocorticoid excess or deficiency...
  6. ncbi Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Chi Ching Hwang
    Department of Biochemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    J Biol Chem 280:3522-8. 2005
    ....
  7. pmc Transgenic amplification of glucocorticoid action in adipose tissue causes high blood pressure in mice
    Hiroaki Masuzaki
    Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Clin Invest 112:83-90. 2003
    ..The potential role of adipose 11beta-HSD1 in mediating critical features of the metabolic syndrome extends beyond obesity and metabolic complications to include the most central cardiovascular feature of this disorder...
  8. ncbi Novel whole-cell biocatalysts with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid
    Michael Braun
    Institute of Biochemical Engineering, Technische Universitat Munchen, Boltzmannstr 15, 85748 Garching, Germany
    Appl Microbiol Biotechnol 95:1457-68. 2012
    ..Various biocatalytic alternatives have already been discussed with hydroxysteroid dehydrogenases (HSDH) playing a crucial role...
  9. ncbi Androgens, estrogens, and hydroxysteroid dehydrogenases
    Dario Mizrachi
    Divisions of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8857, USA
    Mol Cell Endocrinol 301:37-42. 2009
    ..and activity of potent androgens and estrogens in human physiology is largely relegated to the hydroxysteroid dehydrogenases (HSDs)...
  10. ncbi Mechanism of proton transfer in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Yi Hsun Chang
    Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih Chuan 1st Road, Kaohsiung, Taiwan
    J Biol Chem 282:34306-14. 2007
    ..Furthermore, a proton inventory on V/Et is bowl-shaped for both the wild-type and K159A mutant enzymes and indicates a two-proton transfer in the transition state from Tyr(155) to Lys(159) via 2'-OH of ribose...
  11. ncbi Contributions of active site residues to cofactor binding and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase
    Yi Hsun Chang
    Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    Biochim Biophys Acta 1804:235-41. 2010
    ..Substitution of alanine for N86 has a minor effect on NADH binding through K159, resulting in a slight increase in the mobility of the bound NADH and decreases in affinity and catalytic constant...
  12. ncbi Role of S114 in the NADH-induced conformational change and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
    Yi Hsun Chang
    Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80731, Taiwan
    Biochim Biophys Acta 1794:1459-66. 2009
    ..Substitution of alanine for S114 eliminates the hydrogen bonding interaction with P185, causing a conformational change in a nonproductive binding of NADH and a significant loss of activity...
  13. ncbi The 11beta-hydroxysteroid dehydrogenases: functions and physiological effects
    Z Krozowski
    Laboratory of Molecular Hypertension, Baker Medical Research Institute, Melbourne, Australia
    Mol Cell Endocrinol 151:121-7. 1999
    ..11Beta-HSD2 may also play important roles in pulmonary physiology and breast cancer. This review focuses on recent developments...
  14. ncbi Hydroxysteroid dehydrogenases (HSDs) in bacteria: a bioinformatic perspective
    Michael Kisiela
    Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig Holstein, Kiel, Germany
    J Steroid Biochem Mol Biol 129:31-46. 2012
    ..b>Hydroxysteroid dehydrogenases (HSDs), which belong to the superfamily of short-chain dehydrogenases/reductases (SDR) or aldo-keto ..
  15. ncbi Effects of the 11 beta-hydroxysteroid dehydrogenase inhibitor carbenoxolone on insulin sensitivity in men with type 2 diabetes
    Robert C Andrews
    University of Edinburgh, Endocrinology Unit, Department of Medical Sciences, Western General Hospital, Edinburgh, United Kingdom EH4 2XU
    J Clin Endocrinol Metab 88:285-91. 2003
    ..However, clinically useful therapeutic effects will probably require selective 11 beta-HSD1 inhibitors that lower intraadipose cortisol levels and enhance peripheral glucose uptake...
  16. ncbi NAD(+)-dependent isoform of 11 beta-hydroxysteroid dehydrogenase. Cloning and characterization of cDNA from sheep kidney
    A K Agarwal
    Department of Pediatrics, New York Hospital Cornell Medical Center, New York 10021
    J Biol Chem 269:25959-62. 1994
    ..In humans, this gene is a candidate locus for the syndrome of apparent mineralocorticoid excess, a form of hypertension postulated to result from 11-HSD deficiency in mineralocorticoid target tissues...
  17. ncbi The N-terminal anchor sequences of 11beta-hydroxysteroid dehydrogenases determine their orientation in the endoplasmic reticulum membrane
    A Odermatt
    Division of Nephrology, Department of Medicine, University of Berne, 3010 Berne, Switzerland
    J Biol Chem 274:28762-70. 1999
    ..Our results emphasize the importance of the N-terminal transmembrane segments of 11beta-HSD enzymes for their proper function and demonstrate that they are sufficient to determine their orientation in the ER membrane...
  18. ncbi Distribution of glucocorticoid and mineralocorticoid receptors and 11beta-hydroxysteroid dehydrogenases in human and rat ocular tissues
    J Stokes
    Department of Medicine, University of Edinburgh, Western General Hospital, Scotland
    Invest Ophthalmol Vis Sci 41:1629-38. 2000
    ....
  19. ncbi Carbonyl reductases and pluripotent hydroxysteroid dehydrogenases of the short-chain dehydrogenase/reductase superfamily
    Frank Hoffmann
    Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig Holstein, Campus Kiel, Brunswiker Strasse, Kiel, 10, 24105, Germany
    Drug Metab Rev 39:87-144. 2007
    ..In addition, there exist a variety of pluripotent hydroxysteroid dehydrogenases (HSDs) of both superfamilies that specifically catalyze the oxidoreduction at different positions of ..
  20. ncbi Selective inhibition of 11beta-hydroxysteroid dehydrogenase type 1 decreases blood glucose concentrations in hyperglycaemic mice
    P Alberts
    Pharmacology 2, Department of Biology, Research, Biovitrum, Stockholm, Sweden
    Diabetologia 45:1528-32. 2002
    ..The aim of the present study is to test if selective inhibition of 11beta-hydroxysteroid dehydrogenase type 1 lowers blood glucose concentrations in a hyperglycaemic and hyperinsulinaemic mouse model...
  21. ncbi Structure-function relationships in 3alpha-hydroxysteroid dehydrogenases: a comparison of the rat and human isoforms
    Trevor M Penning
    Department of Pharmacology, School of Medicine, University of Pennsylvania, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Steroid Biochem Mol Biol 85:247-55. 2003
    3alpha-Hydroxysteroid dehydrogenases (3alpha-HSDs) inactivate steroid hormones in the liver, regulate 5alpha-dihydrotestosterone (5alpha-DHT) levels in the prostate, and form the neurosteroid, allopregnanolone in the CNS...
  22. ncbi Selective reduction of AKR1C2 in prostate cancer and its role in DHT metabolism
    Qing Ji
    Division of Gastrointestinal and Liver Disease, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Prostate 54:275-89. 2003
    ..Functional activity and regulation of AKR1C2, a 3 alpha-hydroxysteroid dehydrogenase (HSD) type III, was also assessed in prostate cancer cell lines...
  23. ncbi Hydroxysteroid dehydrogenases in bovine and porcine granulosa cells convert zearalenone into its hydroxylated metabolites alpha-zearalenol and beta-zearalenol
    H Malekinejad
    Department of Veterinary Pharmacology, Pharmacy and Toxicology, Utrecht University, Yalelaan 16, 3508 TD, Utrecht, The Netherlands
    Vet Res Commun 30:445-53. 2006
    ..This is the first report demonstrating the extrahepatic biotransformation of zearalenone in target tissues...
  24. pmc Human hydroxysteroid dehydrogenases and pre-receptor regulation: insights into inhibitor design and evaluation
    Trevor M Penning
    Center of Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    J Steroid Biochem Mol Biol 125:46-56. 2011
    b>Hydroxysteroid dehydrogenases (HSDs) represent a major class of NAD(P)(H) dependent steroid hormone oxidoreductases involved in the pre-receptor regulation of hormone action...
  25. ncbi Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization in adult albino rats
    Kuladip Jana
    Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Calcutta 700 037, West Bengal, India
    Contraception 73:289-300. 2006
    ..To investigate a method of chemical sterilization and its efficacy in adult albino rats...
  26. ncbi Several homozygous mutations in the gene for 11 beta-hydroxysteroid dehydrogenase type 2 in patients with apparent mineralocorticoid excess
    R C Wilson
    Department of Pediatrics, New York Hospital Cornell Medical Center, New York 10021, USA
    J Clin Endocrinol Metab 80:3145-50. 1995
    ..This results from consanguinity in two families and possibly from endogamy or a founder effect in four of the other five families. The absence of compound heterozygotes remains to be investigated...
  27. ncbi cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine, Los Angeles 90033
    J Biol Chem 268:10448-57. 1993
    ..Future studies will define the physiologic significance of this novel, multifunctional protein in bile acid transport and xenobiotic metabolism...
  28. ncbi Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization of male Black Bengal goats (Capra hircus): a dose-dependent study
    Kuladip Jana
    Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, 64 1 14 Belgachia Road Milk Colony, 37 and 68 K B Sarani, Calcutta 700037, West Bengal, India
    Anim Reprod Sci 86:89-108. 2005
    ..Changes in these parameters were not significant. An intratesticular injection of calcium chloride at specified doses could be a suitable method of sterilization in preference to surgical castration of goats...
  29. ncbi Hydroxysteroid dehydrogenases: ancient and modern regulators of adrenal and sex steroid action
    M E Baker
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0823, USA
    Mol Cell Endocrinol 175:1-4. 2001
    ..We propose that hydroxysteroid dehydrogenases that metabolized functional groups at different sites on steroids (e.g...
  30. ncbi Mineralocorticoid activity of liquorice: 11-beta-hydroxysteroid dehydrogenase deficiency comes of age
    P M Stewart
    Department of Medicine, Western General Hospital, Edinburgh
    Lancet 2:821-4. 1987
    ....
  31. ncbi Cortisol metabolism in healthy young adults: sexual dimorphism in activities of A-ring reductases, but not 11beta-hydroxysteroid dehydrogenases
    M J Finken
    University of Edinburgh, Department of Medical Sciences, Western General Hospital, United Kingdom
    J Clin Endocrinol Metab 84:3316-21. 1999
    ..by A-ring reductases (5alpha- and 5beta-reductases) and reversibly (to cortisone) by 11beta-hydroxysteroid dehydrogenases (11betaHSDs). In rats, estradiol down-regulates 11betaHSD1 expression...
  32. ncbi Increased expression of genes converting adrenal androgens to testosterone in androgen-independent prostate cancer
    Michael Stanbrough
    Cancer Biology Program, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Cancer Res 66:2815-25. 2006
    ..These results indicate that enhanced intracellular conversion of adrenal androgens to testosterone and dihydrotestosterone is a mechanism by which prostate cancer cells adapt to androgen deprivation and suggest new therapeutic targets...
  33. ncbi Phenotypic analysis of mice bearing targeted deletions of 11beta-hydroxysteroid dehydrogenases 1 and 2 genes
    M C Holmes
    Molecular Endocrinology, Molecular Medicine Centre, Western General Hospital, Edinburgh, UK
    Mol Cell Endocrinol 171:15-20. 2001
    The glucocorticoid metabolising enzymes, 11beta-hydroxysteroid dehydrogenases (11beta-HSD), play a critical role in determining the availability of glucocorticoids to activate their receptors and hence modulate target gene transcription...
  34. ncbi Crystal structure of human type III 3alpha-hydroxysteroid dehydrogenase/bile acid binding protein complexed with NADP(+) and ursodeoxycholate
    Y Jin
    Department of Pharmacology, Department of Biochemistry and Biophysics, and The Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 40:10161-8. 2001
    ....
  35. ncbi SDR-type human hydroxysteroid dehydrogenases involved in steroid hormone activation
    Xiaoqiu Wu
    Structural Genomics Consortium, University of Oxford, Oxford OX3 7LD, United Kingdom
    Mol Cell Endocrinol 265:71-6. 2007
    b>Hydroxysteroid dehydrogenases catalyze the NAD(P)(H)-dependent oxidoreduction of hydroxyl and oxo-functions at distinct positions of steroid hormones...
  36. ncbi Growth hormone replacement inhibits renal and hepatic 11 beta-hydroxysteroid dehydrogenases in ACTH-deficient patients
    B R Walker
    University of Edinburgh, Department of Medicine, Western General Hospital, UK
    Clin Endocrinol (Oxf) 49:257-63. 1998
    ..In this study, we examined the effects of GH replacement on cortisol metabolism...
  37. ncbi Distinguishing the activities of 11beta-hydroxysteroid dehydrogenases in vivo using isotopically labeled cortisol
    Ruth Andrew
    Endocrinology Unit, Department of Medical Sciences, University of Edinburgh, Western General Hospital, Edinburgh, Scotland EH4 2XU, United Kingdom
    J Clin Endocrinol Metab 87:277-85. 2002
    ..This technique offers a significant advance in the methods available to measure turnover in 11betaHSDs and isozymes of 11betaHSDs in vivo in human studies, and this study confirms that carbenoxolone inhibits both isozymes of 11betaHSD...
  38. ncbi Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, Laval University, Quebec, Canada G1V 4G2
    J Mol Biol 369:525-40. 2007
    ..steroid-binding mode for an AKR is similar to that adopted by other steroid-binding proteins, the hydroxysteroid dehydrogenases of the short-chain dehydrogenases/reductases (SDR) family and the steroid hormone nuclear receptors...
  39. ncbi 11beta-hydroxysteroid dehydrogenases, cell proliferation and malignancy
    E H Rabbitt
    Department of Endocrinology, Division of Medical Sciences, Institute of Clinical Research, Queen Elizabeth Hospital, The University of Birmingham, Birmingham B15 2TH, UK
    J Steroid Biochem Mol Biol 85:415-21. 2003
    ..These and other issues are discussed in the present review...
  40. ncbi A cluster of eight hydroxysteroid dehydrogenase genes belonging to the aldo-keto reductase supergene family on mouse chromosome 13
    Laurent Vergnes
    Department of Human Genetics and Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    J Lipid Res 44:503-11. 2003
    A subclass of hydroxysteroid dehydrogenases (HSD) are NADP(H)-dependent oxidoreductases that belong to the aldo-keto reductase (AKR) superfamily...
  41. ncbi Evolution of 17beta-hydroxysteroid dehydrogenases and their role in androgen, estrogen and retinoid action
    M E Baker
    Department of Medicine, 0823 University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0823, USA
    Mol Cell Endocrinol 171:211-5. 2001
    17beta-Hydroxysteroid dehydrogenases (17beta-HSDs) regulate androgen and estrogen concentrations in mammals...
  42. ncbi Characterization of two isoforms of mouse 3(17)alpha-hydroxysteroid dehydrogenases of the aldo-keto reductase family
    Syuhei Ishikura
    Laboratory of Biochemistry, Gifu Pharmaceutical University, Japan
    Biol Pharm Bull 27:1939-45. 2004
    Mouse kidney contains two 3(17)alpha-hydroxysteroid dehydrogenases (HSDs) that show essentially the same properties except for their isoelectric points...
  43. ncbi Xanthomonas maltophilia CBS 897.97 as a source of new 7beta- and 7alpha-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of bile acids
    Paola Pedrini
    Dipartimento delle Risorse Naturali e Culturali, Universita di Ferrara, C so Ercole I d Este 32, I 44100 Ferrara, Italy
    Steroids 71:189-98. 2006
    The paper reports the partial purification and characterization of the 7beta- and 7alpha-hydroxysteroid dehydrogenases (HSDH) and cholylglycine hydrolase (CGH), isolated from Xanthomonas maltophilia CBS 897.97...
  44. ncbi The effect of allelic variation in aldo-keto reductase 1C2 on the in vitro metabolism of dihydrotestosterone
    Ryan H Takahashi
    Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, University of British Columbia, 2146 East Mall, Vancouver, BC, Canada V6T 1Z3
    J Pharmacol Exp Ther 329:1032-9. 2009
    ..These variant AKR1C2 alleles may represent one factor involved in the variable degradation of DHT in vivo...
  45. pmc Human cytosolic hydroxysteroid dehydrogenases of the aldo-ketoreductase superfamily catalyze reduction of conjugated steroids: implications for phase I and phase II steroid hormone metabolism
    Yi Jin
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    J Biol Chem 284:10013-22. 2009
    ....
  46. ncbi Evolution of mammalian 11beta- and 17beta-hydroxysteroid dehydrogenases-type 2 and retinol dehydrogenases from ancestors in Caenorhabditis elegans and evidence for horizontal transfer of a eukaryote dehydrogenase to E. coli
    M E Baker
    Department of Medicine, University of California, San Diego, La Jolla 92093 0623, USA
    J Steroid Biochem Mol Biol 66:355-63. 1998
    ..Sequence comparisons and phylogenetic analysis indicate that the E. coli protein may be an example of horizontal transfer from a eukaryote ancestor...
  47. ncbi An intracellular protein that binds amyloid-beta peptide and mediates neurotoxicity in Alzheimer's disease
    S D Yan
    Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
    Nature 389:689-95. 1997
    ..By interacting with intracellular amyloid-beta, ERAB may therefore contribute to the neuronal dysfunction associated with Alzheimer's disease...
  48. ncbi Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme
    Frédérick Faucher
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Quebec QC, Canada G1V 4G2
    J Mol Biol 364:747-63. 2006
    ....
  49. ncbi Pathophysiology of modulation of local glucocorticoid levels by 11beta-hydroxysteroid dehydrogenases
    T C Sandeep
    Endocrinology Unit, Dept of Medical Sciences, University of Edinburgh, Western General Hospital, Edinburgh, UK EH4 2XU
    Trends Endocrinol Metab 12:446-53. 2001
    11beta-Hydroxysteroid dehydrogenases (11beta HSDs) are enzymes that catalyse the interconversion of active glucocorticoids (cortisol and corticosterone) into their inactive 11-keto products (cortisone and 11-deoxycorticosterone)...
  50. ncbi Hydroxysteroid dehydrogenases and pre-receptor regulation of steroid hormone action
    Trevor M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Hum Reprod Update 9:193-205. 2003
    ..This pre-receptor regulation can be achieved by hydroxysteroid dehydrogenases (HSDs)...
  51. ncbi Purification of full-length recombinant human and rat type 1 11beta-hydroxysteroid dehydrogenases with retained oxidoreductase activities
    C Stefan I Nobel
    Biovitrum AB, Division of Pharmaceuticals, Department of Assay Development and Screening, S 112 87 Stockholm, Sweden
    Protein Expr Purif 26:349-56. 2002
    ..Both enzymes were 90-95% pure and exhibited dehydrogenase and reductase activities with K(M) values in agreement with those reported in the literature...
  52. ncbi Morphologic change and elevation of cortisol secretion in cultured human normal adrenocortical cells caused by mutant p21K-ras protein
    Chia Hung Wu
    Graduate Institute of Biochemistry, Kaohsiung Medical University Kaohsiung, Taiwan, ROC
    DNA Cell Biol 21:21-9. 2002
    ..These results, combined with previous reports of steroidogenic K-ras in bovine adrenocortical cells, suggest that the K-ras oncogene is involved in steroidogenesis in human adrenocortical cells...
  53. ncbi Transcript profiling of the androgen signal in normal prostate, benign prostatic hyperplasia, and prostate cancer
    David R Bauman
    Department of Pharmacology, Center of Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, 130C John Morgan Building, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104 6084, USA
    Endocrinology 147:5806-16. 2006
    ..280 in normal PSC) were maintained in PEC and PSC in diseased prostate. These data suggest that CaP may be more responsive to an ERbeta agonist and BPH may be more responsive to androgen ablation...
  54. ncbi Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution
    I Dufort
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Quebec G1V 4G2, Canada
    J Clin Endocrinol Metab 86:841-6. 2001
    3 alpha-Hydroxysteroid dehydrogenases (3 alpha-HSDs) catalyze the conversion of 3-ketosteroids to 3 alpha-hydroxy compounds...
  55. ncbi Changes in the activities of hydroxysteroid dehydrogenases in mouse oocytes during meiotic maturation
    Sueo Niimura
    Faculty of Agriculture, Niigata University, Japan
    J Reprod Dev 49:451-6. 2003
    The activities of hydroxysteroid dehydrogenases (HSDs) were histochemically demonstrated in mouse oocytes in the process of maturation in vivo and in vitro, and the changes in steroid metabolism during meiotic maturation and also the ..
  56. ncbi Acute intrarenal administration of cortisol has no effect on renal blood flow in hypertensive individuals
    Stan H M van Uum
    Division of General Internal Medicine, Division of Endocrinology, Department of Medicine, University Medical Center, Nijmegen, The Netherlands
    J Hypertens 20:2275-83. 2002
    ..Cortisol is known to increase blood pressure. One possible mechanism is the reported increase in renal vascular resistance (RVR). It is unknown whether this is due to a direct effect of cortisol on the kidneys...
  57. ncbi Antenatal dexamethasone: effect on ovine placental 11beta-hydroxysteroid dehydrogenase type 2 expression and fetal growth
    Leslie S Kerzner
    Brown University School of Medicine, Department of Pediatrics, Women and Infants Hospital of Rhode Island, Providence 02905, USA
    Pediatr Res 52:706-12. 2002
    ..05). We conclude that maternal antenatal dexamethasone treatment reduces placental 11beta-HSD-2 expression and fetal weight at mid-gestation in the ovine pregnancy...
  58. ncbi Furosemide and 11beta-hydroxysteroid dehydrogenase activity, in man
    M Palermo
    Institute of Endocrinology, University of Sassari, Sassari, Italy
    Exp Clin Endocrinol Diabetes 110:272-6. 2002
    ..Our results suggest that furosemide does not play a significant role in 11 beta-OHSD modulation in humans, at least at the dosage used in clinical practice...
  59. ncbi Inhibition of 11beta-hydroxysteroid dehydrogenase type 1 lowers intraocular pressure in patients with ocular hypertension
    S Rauz
    Academic Unit of Ophthalmology, Division of Immunity and Infection, University of Birmingham, Birmingham, UK
    QJM 96:481-90. 2003
    ....
  60. ncbi Increased ratio of serum cortisol to cortisone in acute-phase response
    M Vogeser
    Institute of Clinical Chemistry, Ludwig Maximilians Universitat, Klinikum Grosshadern, Munich, Germany
    Horm Res 58:172-5. 2002
    ..The aim of this pilot study was to investigate whether the serum cortisol:cortisone ratio is altered during the acute-phase response, possibly due to altered modulation of 11beta-hydroxysteroid dehydrogenase isoforms...
  61. ncbi Regulation of 11beta-hydroxysteroid dehydrogenase type 1 gene expression in human ovarian surface epithelial cells by interleukin-1
    Peter Y K Yong
    Assisted Conception Programme, Royal Infirmary of Edinburgh, Edinburgh EH16 4SA, UK
    Hum Reprod 17:2300-6. 2002
    ..Human 11betaHSD type 1 (11betaHSD1) is principally an 11-oxoreductase that reversibly reduces cortisone to cortisol...
  62. ncbi 11beta-hydroxysteroid dehydrogenase activity in proteinuric patients and the effect of angiotensin-II receptor blockade
    M N Kerstens
    University Hospital Groningen, The Netherlands
    Eur J Clin Invest 32:513-8. 2002
    ..We studied the parameters of 11betaHSD activity in proteinuric patients, in particular its activity at the kidney level. We also studied the effect of angiotensin-II receptor blockade on the parameters of 11betaHSD activity...
  63. ncbi Measurement of cortisol secretion rate by stable isotope methodology following oral administration of 13C-labeled cortisol to a human subject
    Yasuji Kasuya
    Department of Medicinal Chemistry and Clinical Pharmacy, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo, Japan
    Steroids 67:783-8. 2002
    ....
  64. ncbi 11beta-Hydroxysteroid dehydrogenase types 1 and 2 are up- and downregulated in cortisol-secreting adrenal adenomas
    Giuseppina Mazzocchi
    Department of Human Anatomy and Physiology, School of Medicine, University of Padua, Italy
    J Investig Med 50:288-92. 2002
    ..In contrast, 11betaHSD1 has been investigated to a much lesser degree. We therefore studied and compared the expression and activity of the two isoenzymes in the human adrenal cortex (HAC) and cortisol-secreting adenomas (CSAs)...
  65. ncbi Enantioselectivity of carbonyl reduction of 4-methylnitrosamino-1-(3-pyridyl)-1-butanone by tissue fractions from human and rat and by enzymes isolated from human liver
    Ursula Breyer-Pfaff
    Department of Pharmacology and Toxicology, University of Tubingen, Germany
    Drug Metab Dispos 32:915-22. 2004
    ..Rat liver and lung microsomes produced NNAL with about 33% and 55% (R)-enantiomer and a mean contribution of 11beta-HSD1 of 12% and 32%, respectively. Multiple enzymes seem to participate in NNK reduction in human and rat tissues...
  66. ncbi Chenodeoxycholic acid and deoxycholic acid inhibit 11 beta-hydroxysteroid dehydrogenase type 2 and cause cortisol-induced transcriptional activation of the mineralocorticoid receptor
    Anita T Stauffer
    Division of Nephrology and Hypertension, Department of Clinical Research, University of Berne, 3010 Berne, Switzerland
    J Biol Chem 277:26286-92. 2002
    ....
  67. ncbi Expression of steroid-converting enzymes in osteoblasts derived from rat vertebrae
    Y Ishida
    Faculty of Dentistry, University of Toronto, Ontario, Canada
    Osteoporos Int 13:235-40. 2002
    ....
  68. ncbi [Tissue-specific changes in cortisol metabolism and their potential role in the metabolic syndrome]
    M N Kerstens
    Universitair Medisch Centrum Groningen, Afd Endocrinologie, Postbus 30 001, 9700 RB Groningen
    Ned Tijdschr Geneeskd 149:871-6. 2005
    ..Pharmacological inhibition of IIbettaHSD type I activity provides an interesting mechanism for the development of novel therapeutic agents for type-2 diabetes mellitus...
  69. ncbi The role of 11beta-hydroxysteroid dehydrogenase activity in the metabolic syndrome: lessons learned from the animal model
    Jorg Dotsch
    Klinik fur Kinder und Jugendliche, Loschgestr 15, 91054 Erlangen, Friedrich Alexander Universitat Erlangen Nurnberg, Germany
    Eur J Endocrinol 146:603-5. 2002
  70. ncbi Hypertension and the cortisol-cortisone shuttle
    Marcus Quinkler
    Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
    J Clin Endocrinol Metab 88:2384-92. 2003
    ..At a prereceptor level, 11 beta-HSD2 plays a key role in normal physiology in the corticosteroid regulation of sodium homeostasis and pathophysiology of hypertension...
  71. ncbi Localization of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in the male reproductive tract
    Brendan J Waddell
    School of Anatomy and Human Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, Western Australia 6009, Australia
    Endocrinology 144:3101-6. 2003
    ....
  72. ncbi Expression of 11beta-hydroxysteroid dehydrogenase type 1 in adipose tissue is not increased in human obesity
    J W Tomlinson
    Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, Edgbaston, Birmingham, United Kingdom B15 2TH
    J Clin Endocrinol Metab 87:5630-5. 2002
    ..05). Enhanced preadipocyte proliferation within omental adipose tissue as a consequence of decreased 11beta-HSD1 mRNA levels and activity may contribute to increases in visceral adipose tissue mass in obese patients...
  73. ncbi Role of local 11 beta-hydroxysteroid dehydrogenase type 2 expression in determining the phenotype of adrenal adenomas
    Tomoatsu Mune
    Third Department of Internal Medicine, Gifu University School of Medicine, Gifu 500 8705, Japan
    J Clin Endocrinol Metab 88:864-70. 2003
    ..Nonfunctioning adenomas and those causing preclinical and overt Cushing's syndrome may represent a continuum with clinical manifestations depending mainly on tumor size and HSD11B2 expression levels...
  74. ncbi [11 beta-hydroxysteroid dehydrogenase type 2 activity in Chilean patients with hypertension]
    Lorena Mosso
    Departamentos de Endocrinología y Medicina Interna, Centro de Investigaciones Medicas, Escuela de Medicina, Pontificia Universidad Catolica de Chile
    Rev Med Chil 130:1201-8. 2002
    ..If the enzyme fails, cortisol will stimulate sodium and water reabsorption and increase blood pressure...
  75. ncbi Hypoxia causes down-regulation of 11 beta-hydroxysteroid dehydrogenase type 2 by induction of Egr-1
    Christian D Heiniger
    Division of Nephrology and Hypertension, University of Berne, 3010 Berne, Switzerland
    FASEB J 17:917-9. 2003
    ..Thus, hypoxia decreases 11beta-HSD2 transcription and activity by inducing Egr-1 in vivo and in vitro. This mechanism might account for enhanced renal sodium retention and hypertension associated with hypoxic conditions...
  76. ncbi Greater replication and differentiation of preadipocytes in inherited corticosteroid-binding globulin deficiency
    J M Joyner
    Department of Medicine, Redland Hospital, Cleveland, Queensland 4163, Australia
    Am J Physiol Endocrinol Metab 284:E1049-54. 2003
    ..Furthermore, these observations may be relevant to obesity occurring with suppressed CBG concentrations associated with hyperinsulinemia...
  77. ncbi Enhanced 11beta-hydroxysteroid dehydrogenase type 1 activity in stress adaptation in the guinea pig
    M Quinkler
    Department of Endocrinology, Klinikum Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    J Endocrinol 176:185-92. 2003
    The 11beta-hydroxysteroid dehydrogenases (11beta-HSDs) convert cortisol to its inactive metabolite cortisone and vice versa...
  78. ncbi Gestation-related and betamethasone-induced changes in 11beta-hydroxysteroid dehydrogenase types 1 and 2 in the baboon placenta
    Xiao Hong Ma
    Laboratory of Pregnancy and Newborn Research, Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Am J Obstet Gynecol 188:13-21. 2003
    ..We examined whether maternal glucocorticoid administration alters placental 11beta-HSD 2 expression...
  79. ncbi Regulation of 11 beta-hydroxysteroid dehydrogenase enzymes in the rat kidney by estradiol
    Elise P Gomez-Sanchez
    Endocrine Section and Research Service, G V Sonny Montgomery Veterans Affairs Medical Center, Jackson, MS 39216, USA
    Am J Physiol Endocrinol Metab 285:E272-9. 2003
    ..NAD+-dependent enzymatic activity in the absence or presence of a reducing agent demonstrated that the increase in 11betaHSD2 protein was not associated with an increase in in vitro activity unless the dimers were reduced to monomers...
  80. ncbi Low-dose growth hormone inhibits 11 beta-hydroxysteroid dehydrogenase type 1 but has no effect upon fat mass in patients with simple obesity
    Jeremy W Tomlinson
    Division of Medical Sciences, Queen Elizabeth Hospital, University of Birmingham, Birmingham, United Kingdom B15 2TH
    J Clin Endocrinol Metab 88:2113-8. 2003
    ..09, GH vs. placebo, P = 0.07). Treatment with low-dose GH in obesity fails to alter fat mass despite a significant elevation in IGF-I and a shift in the global set point of E to F conversion consistent with inhibition of 11 beta-HSD1...
  81. ncbi Short-term cortisol infusion in the brachial artery, with and without inhibiting 11 beta-hydroxysteroid dehydrogenase, does not alter forearm vascular resistance in normotensive and hypertensive subjects
    S H M van Uum
    Department of Medicine, University Medical Center Nijmegen, Nijmegen, The Netherlands
    Eur J Clin Invest 32:874-81. 2002
    ..We studied the acute effect of cortisol on forearm vascular resistance (FVR) by infusing cortisol directly into the brachial artery, both with and without inhibition of 11 beta-HSD, in normotensive and hypertensive subjects...
  82. ncbi Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles
    L M Thurston
    Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School, University College London, Rowland Hill Street, London NW3 2PF, UK
    Reproduction 124:801-12. 2002
    ....
  83. ncbi Placental 11 beta-hydroxysteroid dehydrogenase-2 and fetal cortisol/cortisone shuttle in small preterm infants
    Eero Kajantie
    Hospital for Children and Adolescents, Helsinki University Central Hospital, 00029 HUS, Helsinki, Finland
    J Clin Endocrinol Metab 88:493-500. 2003
    ..Whether these conditions are associated with early postnatal adrenal insufficiency or long-term cardiovascular risk remains an important issue for further study...
  84. ncbi 11 beta-Hydroxysteroid dehydrogenase activity in hypothalamic obesity
    Dov Tiosano
    Departments of Pediatrics, Meyer Children s Hospital, Haifa 31096, Israel
    J Clin Endocrinol Metab 88:379-84. 2003
    ..We propose that deficiency of hypothalamic messengers after surgical injury induces a paracrine/autocrine effect of enhanced glucocorticoid activity due to up-regulated 11 beta-HSD1 activity...
  85. ncbi Placental 11beta-hydroxysteroid dehydrogenase in Dahl and spontaneously hypertensive rats
    Karla Mazancova
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Am J Hypertens 16:401-6. 2003
    ..It cannot be excluded, therefore, that the placental glucocorticoid barrier in Dahl rats influences the pathways that might lead to the sensitivity of blood pressure to high salt intake in later life...
  86. ncbi Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum
    M Tetsuka
    Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan 080 8555
    J Endocrinol 177:445-52. 2003
    ..local concentrations of active glucocorticoid are determined by the relative expression of two 11beta-hydroxysteroid dehydrogenases (HSDs): bi-directional 11beta-HSD type1 (11HSD1) that mainly activates cortisone to cortisol, and ..
  87. ncbi Increased cortisol metabolites and reduced activity of 11beta-hydroxysteroid dehydrogenase in patients on hemodialysis
    Verena N'Gankam
    Division of Nephrology and Hypertension, University of Berne, Berne, Switzerland
    Kidney Int 61:1859-66. 2002
    ..We hypothesized that first, these metabolites accumulate and second, their concentration pattern changes in patients on hemodialysis...
  88. ncbi Human adrenal cortex and aldosterone secreting adenomas express both 11beta-hydroxysteroid dehydrogenase type 1 and type 2 genes
    Giovanna Albertin
    Department of Human Anatomy and Physiology, Section of Anatomy, University of Padua, I 35121 Padua, Italy
    Int J Mol Med 9:495-8. 2002
    ....
  89. ncbi Reduced 11beta-hydroxysteroid dehydrogenase type 2 activity is associated with decreased birth weight centile in pregnancies complicated by asthma
    Vanessa E Murphy
    Mothers and Babies Research Centre, University of Newcastle, New South Wales, Australia
    J Clin Endocrinol Metab 87:1660-8. 2002
    ....
  90. ncbi Increased ACTH levels do not alter renal 11beta-hydroxysteroid dehydrogenase type 2 gene expression in the sheep
    A L Albiston
    Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia
    Endocr J 48:119-22. 2001
    ....
  91. ncbi The intracellular localization of the mineralocorticoid receptor is regulated by 11beta-hydroxysteroid dehydrogenase type 2
    A Odermatt
    Department of Clinical Research, Division of Nephrology and Hypertension, University of Berne, 3010 Berne, Switzerland
    J Biol Chem 276:28484-92. 2001
    ..Our results provide evidence that 11beta-HSD2, besides inactivating 11beta-hydroxyglucocorticoids, functionally interacts with the MR and directly regulates the magnitude of aldosterone-induced MR activation...
  92. ncbi Apparent mineralocorticoid excess
    R C Wilson
    Pediatric Endocrinology, New York-Presbyterian Hospital and the Weill Medical College of Cornell University, 525 East 68th Street, New York, NY 10021, USA
    Trends Endocrinol Metab 12:104-11. 2001
    ..In 1998, a mild form of this disease was reported, which might represent an important cause of low-renin hypertension. Early and vigilant treatment might prevent or improve the morbidity and mortality of end-organ damage...
  93. ncbi Expression and NNK reducing activities of carbonyl reductase and 11beta-hydroxysteroid dehydrogenase type 1 in human lung
    C Finckh
    Department of Pharmacology and Toxicology, School of Medicine, Karl von Frisch Strasse 1, Philipps University of Marburg, D 35033, Marburg, Germany
    Chem Biol Interact 130:761-73. 2001
    ....
  94. ncbi Characterization of enzymes participating in carbonyl reduction of 4-methylnitrosamino-1-(3-pyridyl)-1-butanone (NNK) in human placenta
    A Atalla
    Department of Pharmacology and Toxicology, School of Medicine, Philipps-University of Marburg, D-35033, Marburg, Germany
    Chem Biol Interact 130:737-48. 2001
    ..1.1.184) seems to perform this reaction in human placenta cytosol. The presence of carbonyl reductase was confirmed by RT-PCR. This is the first report to provide evidence that NNAL formation in placenta is mediated by carbonyl reductase...
  95. ncbi Guinea pig 11beta-hydroxysteroid dehydrogenase type 1: primary structure and catalytic properties
    X Pu
    The Lawson Research Institute, St Joseph s Health Centre, Departments of Obstetrics and Gynecology and Physiology, University of Western Ontario, 268 Grosvenor Street, London, Ontario, Canada
    Steroids 65:148-56. 2000
    ..Thus, the guinea pig provides a useful model in which the structural determinants of catalytic function of 11beta-HSD1 may be studied...
  96. ncbi [Polycystic ovary syndrome of extra-ovarian origin. Review]
    J Terán Dávila
    Servicio de Salud Reproductiva, Centro Colaborador del Programa Especial de Reproducción Humana de la Organización Mundial de la Salud, Maternidad Concepcion Palacios, Caracas, Venezuela
    Invest Clin 42:51-78. 2001
    ..It is possible to improve the ovarian function in some patients with POS. This could be achieved with clomiphene citrate associated with glucocorticoids to induce ovulation...
  97. ncbi Expression of 11 beta-hydroxysteroid dehydrogenase isozymes and corticosteroid hormone receptors in primary cultures of human trophoblast and placental bed biopsies
    P M Driver
    Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, UK
    Mol Hum Reprod 7:357-63. 2001
    ..The presence of the MR within trophoblast indicates that some of the effects of cortisol could be MR- rather than GR-mediated...
  98. ncbi Tissue-specific dysregulation of cortisol metabolism in human obesity
    E Rask
    Departments of Public Health and Clinical Medicine, , Sweden
    J Clin Endocrinol Metab 86:1418-21. 2001
    ....
  99. ncbi Decreased gene expression of 11beta-hydroxysteroid dehydrogenase type 2 and 15-hydroxyprostaglandin dehydrogenase in human placenta of patients with preeclampsia
    E Schoof
    Department of Pediatrics, University of Erlangen-Nuremberg, 91054 Erlangen, Germany
    J Clin Endocrinol Metab 86:1313-7. 2001
    ..By means of an autocrine or paracrine mechanism, the diminished conversion of placental cortisol may lead to reduced PGDH mRNA expression as found in the present study...
  100. ncbi A mutation in the cofactor-binding domain of 11beta-hydroxysteroid dehydrogenase type 2 associated with mineralocorticoid hypertension
    A Odermatt
    Division of Nephrology and Hypertension, Inselspital, University of Berne, 3010 Berne, Switzerland
    J Clin Endocrinol Metab 86:1247-52. 2001
    ....
  101. ncbi Steroidogenic enzyme gene expression in the human heart
    K M Kayes-Wandover
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235 9063, USA
    J Clin Endocrinol Metab 85:2519-25. 2000
    ..1% those in the adrenal gland. These findings are consistent with autocrine or paracrine roles for corticosterone and deoxycorticosterone, but not cortisol or aldosterone, in the normal adult human heart...

Research Grants40

  1. HUMAN PROSTATIC 3A HYDROXYSTEROID DEHYDROGENASE
    Hsueh Kung Lin; Fiscal Year: 2002
    DESCRIPTION (Adapted from the Applicant's Abstract): In androgen target tissues 3a-hydroxysteroid dehydrogenases (3a-HSDs) may regulate the occupancy of androgen receptor (AR) by interconverting 5a-dihydrotestosterone (5a-DHT, a potent ..
  2. MOLECULAR STUDIES OF A KEY BILE ACID METABOLIZING ENZYME
    Andrew Stolz; Fiscal Year: 1993
    ..We have previously identified that the interactions of bile acids with the cytosolic 3 alpha hydroxysteroid dehydrogenases (HSD) is an important determinant in their ultimate uptake and excretion by hepatocytes...
  3. MECHANISMS AND CHEMISTRY OF DEHYDROGENASE INACTIVATORS
    DOUGLAS COVEY; Fiscal Year: 1992
    b>Hydroxysteroid dehydrogenases (HSD)s constitute an important class of steroid metabolizing enzymes...
  4. ENDOCRINOLOGY OF 17BETA-HYDROXYSTEROID DEHYDROGENASES
    A Andersson; Fiscal Year: 2000
    ..the research proposed are to define the biochemistry, function, and physiological roles of the 17beta-hydroxysteroid dehydrogenases (17beta-HSDs), a group of isozymes crucial to the biosynthesis and activation/inactivation of sex ..
  5. Molecular Mechanisms of Multistage Carcinogenesis
    TREVOR PENNING; Fiscal Year: 2006
    ..By studying the discrete molecular events responsible for the causation of cancer this Program may lead to the early prevention and intervention of this disease. ..
  6. Symposium on Aldo-Keto Reductases & Toxicant Metabolism
    TREVOR PENNING; Fiscal Year: 2002
    ..abstract_text> ..
  7. Renal 11b-Hydroxysteroid Dehydrogenases and Hypertension
    Mingyu Liang; Fiscal Year: 2008
    ..of the pathophysiology of salt-sensitive hypertension by investigating the role of renal 11beta-hydroxysteroid dehydrogenases (11beta-HSDs). 11beta-HSDs are critical controllers of local levels of active glucocorticoids...
  8. ANDROGEN PRODUCTION IN ADRENARCHE AND PCOS
    WALTER MILLER; Fiscal Year: 2006
    ..abstract_text> ..
  9. Functions of Very Large G-protein Coupled Receptor-1
    Perrin White; Fiscal Year: 2008
    ..We are specifically interested in the other proteins with very large ectodomains, protocadherin- 15 and cadherin-23. These studies should shed new light on mechanisms controlling development of the retina and inner ear. ..
  10. REGULATION OF HUMAN ALDOSTERONE SYNTHASE
    Perrin White; Fiscal Year: 2002
    ..These studies should provide insight into mechanisms underlying a genetic risk factor for cardiovascular disease. ..
  11. REGULATION OF INTESTINAL BILE ACID BINDING PROTEIN
    SANDY HWANG; Fiscal Year: 2002
    ..Because BA absorption is immature in human infants as well as in suckling rats, this project may provide clinical application to managing neonates, especially those who are premature. ..
  12. MOLECULAR GENETICS OF LOW RENIN HYPERTENSION
    Perrin White; Fiscal Year: 2001
    ..The investigators will determine the role of 11-HSD K in the development of essential hypertension, using both linkage and association studies. ..
  13. Biochemical Basis of Cortisone Reductase Deficiency
    Perrin White; Fiscal Year: 2007
    ..Subsets of the genotyping data will be analyzed as an association study, as an affected sib pair study, and by transmission disequilibrium testing. Additional polymorphisms in these genes will be sought in PCOS subjects. ..
  14. Xenogen Rodent Imaging System
    JOSEPH NAPOLI; Fiscal Year: 2008
    ..Availability of this instrument would undoubtedly stimulate its use by others on campus and in the area. [unreadable] [unreadable] [unreadable]..
  15. Contribution of ATGL in skeletal muscle to lipid metabolism and insulin action
    ERIN KERSHAW; Fiscal Year: 2008
    ..Evaluating the role of ATGL-mediated fat breakdown in skeletal muscle will provide important insights into the pathophysiology and treatment of obesity, insulin resistance, and related metabolic disorders. [unreadable] [unreadable]..
  16. The Role of 11beta-HSD1 in Type II Diabetes and Obesity
    Yanjun Liu; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  17. Biosynthesis of 9-cis-retinoic acid/retinol homeostasis
    JOSEPH NAPOLI; Fiscal Year: 2007
    ..This work will provide new insight into retinoid metabolism/ function. ..
  18. RETINOID METABOLISM
    JOSEPH NAPOLI; Fiscal Year: 2007
    ..This work will contribute to revealing pathology caused or exacerbated by impaired atRA biosynthesis during carcinogenesis, skin disease, birth defects, or diseases of intermediary metabolism. ..
  19. Cortisol at MR Mediate Fetal Physiologic/Genomic Effects
    Maureen Keller Wood; Fiscal Year: 2007
    ..abstract_text> ..
  20. Adrenal decommissioning and hypertension
    Elise Gomez Sanchez; Fiscal Year: 2007
    ..Using similar protocols as those in Aim 1, we will study and compare the process of adrenal decommissioning between SHR-SP and regular SHR rats with emphasis of the ZG genes identified as important in Aim 1. ..
  21. RETINAL DEHYDROGENASES
    JOSEPH NAPOLI; Fiscal Year: 2009
    ..The fundamental insight and reagents generated will be useful to identify diseases and age-related degeneration that may be caused or exacerbated by impaired RA biosynthesis. ..
  22. Hormones, hormone-related genes, and colorectal cancer
    Jennifer Lin; Fiscal Year: 2008
    ..Findings from the proposed study of estrogen and progesterone will enhance our understanding of these lifestyle and genetic factors in relation to colorectal carcinogenesis. ..
  23. CONTROL OF CORTICOTROPIN DURING PREGNANCY
    Maureen Keller Wood; Fiscal Year: 2006
    ..Finally, the ability of progesterone to inhibit MR activation will be tested in gel-shift and super-shift assays using specific response elements in the 5HT1A promoter, including a nGRE and the SP-1 site. ..
  24. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 2006
    ..Proof of the successful prediction of the structures of 20-30 hypothetical members of the SCOR proteome could provide a model for the prediction of protein folding on the basis of as little as 20% sequence homology. ..
  25. STRUCTURE/FUNCTION OF C1 CHANNEL GENES IN CILIARY BODIES
    Miguel Coca Prados; Fiscal Year: 2001
    ..4) To determine the organization of the human pICln and ClC-3 genes at their 5' flanking region. The goal in these studies will be to obtain information from genomic clones of the putative regulatory elements present in these genes. ..
  26. MATERNAL CORTICOSTEROIDS AND FETAL HOMEOSTASIS
    Maureen Keller Wood; Fiscal Year: 2001
    ..The results of these studies will indicate whether volume homeostasis in the fetus depends on maternal cortisol concentration. ..
  27. Disruption of the SGk1 gene in aldosterone target cells.
    ANIKO NARAY FEJES TOTH; Fiscal Year: 2002
    ..abstract_text> ..
  28. SMITH-LEMLI-OPITZ SYNDROME STEROIDOGENESIS
    Cedric Shackleton; Fiscal Year: 2002
    ..Finally, if ring B dehydrosteroids are produced, is the ratio of the individual dehydrometabolites to corresponding conventional metabolites related to the dehydrocholesterol to cholesterol ratio and clinical severity? ..
  29. STEROIDS /VASCULAR REACTIVITY /NITRIC OXIDE PRODUCTION
    Maureen Keller Wood; Fiscal Year: 2002
    ..These studies will also to our understanding of the counterbalancing effects of increased cortisol and increased estrogens in control of normal blood pressure in normal pregnancy. ..
  30. Gas chromatograph-mass spectrometer with EI and CI
    Cedric Shackleton; Fiscal Year: 2003
    ..g., malondialdehyde) and markers of DNA damage (incorporation of uracil). Dr Kuypers has several projects on the red cell membrane, which require profiling of fatty acids using EI GC/MS. ..
  31. ERAB, A BETA, NEUROTOXICITY AND ALZHEIMERS DISEASE
    Shi Du Yan; Fiscal Year: 2003
    ..abstract_text> ..
  32. FOLDING OF THE INTRACELLULAR LIPID-BINDING PROTEINS
    Ira Ropson; Fiscal Year: 2003
    ..Single-site mutant proteins will be generated and studied for their effects on the transition state in protein folding. ..
  33. ALDOSTERONE, HYPERTENSION AND THE BRAIN
    Elise Gomez Sanchez; Fiscal Year: 2003
    ..4) Study of the metabolism of aldosterone and corticosterone and the regulation of the synthesis of low polarity derivatives in the brain, including the identification of the sites of formation of aldosterone acyl esters. ..
  34. DIFFERENTIAL GENE EXPRESSION IN CILIARY EPITHELIUM
    Miguel Coca Prados; Fiscal Year: 2005
    ..To determine any light-sensing responses in intact and cultured ciliary epithelial cells, by studying the cGMP-gated channel under light and dark conditions. ..
  35. The Role of RAGE and EAE
    Shi Du Yan; Fiscal Year: 2005
    ....
  36. Nutritional Biochemistry of beta-Carotene Dioxygenase
    A Andersson; Fiscal Year: 2005
    ..The studies described in this proposal will further our knowledge on vitamin A synthesis from dietary provitamin A carotenoids in man. ..
  37. Neurosteroids and Smith-Lemli-Optiz Syndrome
    Cedric Shackleton; Fiscal Year: 2005
    ..All studies will require high sensitivity steroid analysis by chemical ionization (CI) GC/MS and HPLC/MS. ..
  38. ALDO SPECIFICITY OF MINERALOCORTICOID TARGET CELLS
    ANIKO NARAY FEJES TOTH; Fiscal Year: 2009
    ..WT mice, we will identify aldosterone-regulated genes that are not downstream of SGK1. CCD cells will be isolated by immuno-selection, and differentially expressed genes identified using DNA microarrays. ..