aldehyde lyases

Summary

Summary: Enzymes that catalyze a reverse aldol condensation. A molecule containing a hydroxyl group and a carbonyl group is cleaved at a C-C bond to produce two smaller molecules (ALDEHYDES or KETONES). EC 4.1.2.

Top Publications

  1. ncbi Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism
    Kenji Matsui
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753 8515, Japan
    Curr Opin Plant Biol 9:274-80. 2006
  2. pmc Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance
    G Vancanneyt
    , Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, , 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 98:8139-44. 2001
  3. ncbi Sphingosine-1-phosphate lyase SPL is an endoplasmic reticulum-resident, integral membrane protein with the pyridoxal 5'-phosphate binding domain exposed to the cytosol
    Mika Ikeda
    Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 jo, Nishi 6 choume, Kita ku, Sapporo 060 0812, Japan
    Biochem Biophys Res Commun 325:338-43. 2004
  4. pmc Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution
    Gavin J Williams
    School of Biochemistry and Molecular Biology, Department of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom
    Proc Natl Acad Sci U S A 100:3143-8. 2003
  5. ncbi Directed evolution of an industrial biocatalyst: 2-deoxy-D-ribose 5-phosphate aldolase
    Stefan Jennewein
    DSM Anti Infectives, Delft, The Netherlands
    Biotechnol J 1:537-48. 2006
  6. ncbi Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts
    Theodora Farmaki
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, Universidad Autonoma de Madrid, Carretera de Colmenar Viejo km 15, 500 28049 Madrid, Spain
    J Exp Bot 58:555-68. 2007
  7. ncbi Silencing of hydroperoxide lyase and allene oxide synthase reveals substrate and defense signaling crosstalk in Nicotiana attenuata
    Rayko Halitschke
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str 8, D 07745 Jena, Germany
    Plant J 40:35-46. 2004
  8. ncbi Lymphocyte sequestration through S1P lyase inhibition and disruption of S1P gradients
    Susan R Schwab
    Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0414, USA
    Science 309:1735-9. 2005
  9. ncbi Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase
    Ningqing Ran
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
    J Am Chem Soc 129:6130-9. 2007
  10. ncbi Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids
    N Tijet
    Department of Pharmacology, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA
    Arch Biochem Biophys 386:281-9. 2001

Research Grants

  1. ENZYME CATALYZED SYNTHESIS OF MONOSACCHARIDES
    George Whitesides; Fiscal Year: 2000
  2. ALDOLASE GENES IN NORMAL AND FRUCTOSE INTOLERANT HUMANS
    DEAN TOLAN; Fiscal Year: 1991
  3. METALLOBIOCHEMISTRY OF VANADIUM(V)
    DEBBIE CRANS; Fiscal Year: 1993
  4. ENZYMES IN ORGANIC SYNTHESIS
    Chi Huey Wong; Fiscal Year: 1999
  5. NOVEL DHAP-DEPENDENT ALDOLASES FOR ORGANIC SYNTHESIS
    FRANCISCO MORIS VARAS; Fiscal Year: 2000
  6. The Evaluation of Synthetically Useful Aldolases
    Eric Toone; Fiscal Year: 2005
  7. MICROBIAL GLYCOLYSIS MUTANTS
    DAN FRAENKEL; Fiscal Year: 1992
  8. Sphingolipid signaling in olfactory mucosa
    Mary Beth Genter; Fiscal Year: 2004
  9. Strategies for Selective Covalent Conjugation of Proteins with Small Molecules
    Fujie Tanaka; Fiscal Year: 2007
  10. Universal Technology for Profiling the Dynamics of Normal & Oncogenic Signaling
    Karen Anderson; Fiscal Year: 2008

Detail Information

Publications199 found, 100 shown here

  1. ncbi Green leaf volatiles: hydroperoxide lyase pathway of oxylipin metabolism
    Kenji Matsui
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753 8515, Japan
    Curr Opin Plant Biol 9:274-80. 2006
    ....
  2. pmc Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance
    G Vancanneyt
    , Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, , 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 98:8139-44. 2001
    ..Thus, HPL-catalyzed production of C6 aldehydes may be a key step of a built-in resistance mechanism of plants against some sucking insect pests...
  3. ncbi Sphingosine-1-phosphate lyase SPL is an endoplasmic reticulum-resident, integral membrane protein with the pyridoxal 5'-phosphate binding domain exposed to the cytosol
    Mika Ikeda
    Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 jo, Nishi 6 choume, Kita ku, Sapporo 060 0812, Japan
    Biochem Biophys Res Commun 325:338-43. 2004
    ..This active site orientation is opposite to that of S1P phosphohydrolase, indicating that the degradation of S1P by two S1P-degrading enzymes occurs in spatially separated sides of the endoplasmic reticulum...
  4. pmc Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution
    Gavin J Williams
    School of Biochemistry and Molecular Biology, Department of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom
    Proc Natl Acad Sci U S A 100:3143-8. 2003
    ..This demonstration is of considerable significance to synthetic chemists requiring efficient syntheses of complex stereoisomeric products, such as carbohydrate mimetics...
  5. ncbi Directed evolution of an industrial biocatalyst: 2-deoxy-D-ribose 5-phosphate aldolase
    Stefan Jennewein
    DSM Anti Infectives, Delft, The Netherlands
    Biotechnol J 1:537-48. 2006
    ..By combination of the most beneficial mutations we obtained a tenfold improved variant compared to wild-type DERA with regard to (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside synthesis, under industrially relevant conditions...
  6. ncbi Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts
    Theodora Farmaki
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, Universidad Autonoma de Madrid, Carretera de Colmenar Viejo km 15, 500 28049 Madrid, Spain
    J Exp Bot 58:555-68. 2007
    ..It is suggested that, in addition to their differential expression pattern, this segregation underlies the regulation of metabolic fluxes through the alternative branches of the lipoxygenase pathway...
  7. ncbi Silencing of hydroperoxide lyase and allene oxide synthase reveals substrate and defense signaling crosstalk in Nicotiana attenuata
    Rayko Halitschke
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str 8, D 07745 Jena, Germany
    Plant J 40:35-46. 2004
    ..Elicitation of volatile terpenoids (an indirect defense) requires JA signaling, where as trypsin protease inhibitor elicitation (a direct defense) requires both functional JA and GLV cascades...
  8. ncbi Lymphocyte sequestration through S1P lyase inhibition and disruption of S1P gradients
    Susan R Schwab
    Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0414, USA
    Science 309:1735-9. 2005
    ..We conclude that lymphocyte egress is mediated by S1P gradients that are established by S1P lyase activity and that the lyase may represent a novel immunosuppressant drug target...
  9. ncbi Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase
    Ningqing Ran
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
    J Am Chem Soc 129:6130-9. 2007
    ..7% molar yield from glucose. Coamplification with transketolase, which increases d-erythrose 4-phosphate availability, afforded 16 g/L 3-dehydroshikimic acid in 8.5% molar yield...
  10. ncbi Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids
    N Tijet
    Department of Pharmacology, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA
    Arch Biochem Biophys 386:281-9. 2001
    ..Overall, the newly characterized cytochrome P450 enzyme is a fatty acid hydroperoxide lyase with a preference, but not absolute specificity for the 9-positional hydroperoxides of linoleic and linolenic acids...
  11. pmc In vivo selection for the directed evolution of L-rhamnulose aldolase from L-rhamnulose-1-phosphate aldolase (RhaD)
    Masakazu Sugiyama
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Bioorg Med Chem 15:5905-11. 2007
    ..An initial round of screening gave mutants allowing the selection strain to grow on MMRha plates. This in vivo selection system allows rapid screening of mutated aldolases that can utilize dihydroxyacetone as a donor substrate...
  12. pmc Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis
    Hui Duan
    Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801, USA
    Plant Physiol 139:1529-44. 2005
    ..Two of the three transcripts coding for aliphatic glucosinolates (CYP83A1, AOP3) are also expressed at significantly lower levels in Col flowers...
  13. ncbi Structure of tagatose-1,6-bisphosphate aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases
    David R Hall
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 277:22018-24. 2002
    ..The availability of more space and a wider range of aldehyde partners used by TBPA together with the highly specific nature of FBPA suggest that TBPA might be a preferred enzyme to modify for use in biotransformation chemistry...
  14. ncbi The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus
    Alex Theodossis
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, Fife KY16 9ST, Scotland
    J Biol Chem 279:43886-92. 2004
    ..It is proposed that the active site of the enzyme is rigid to keep its thermostability but incorporates extra functionality to be promiscuous...
  15. pmc Rice HYDROPEROXIDE LYASES with unique expression patterns generate distinct aldehyde signatures in Arabidopsis
    E W Chehab
    Section of Plant Biology, University of California, Davis, California 95616, USA
    Plant Physiol 141:121-34. 2006
    ..Together, these data suggest that additional tissue-specific mechanism(s) beyond transcript and HPL enzyme abundance, regulate the levels of HPL-derived metabolites...
  16. ncbi Elucidation of the mode of substrate binding to hydroxynitrile lyase from Hevea brasiliensis
    K Gruber
    Institut für Chemie Strukturbiologie, Universitat Graz, Heinrichstrasse 28, A 8010 Graz, Austria
    Proteins 44:26-31. 2001
    ....
  17. ncbi Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis
    U G Wagner
    Institut fur Physikalische Chemie, Universitat Graz, Austria
    Structure 4:811-22. 1996
    ..We set out to gain insight into the catalytic mechanism of this important class of enzymes by determining the three-dimensional structure of hydroxynitrile lyase from the rubber tree, Hevea brasiliensis...
  18. pmc Changing green leaf volatile biosynthesis in plants: an approach for improving plant resistance against both herbivores and pathogens
    Kaori Shiojiri
    Center for Ecological Research, Kyoto University, Otsu 520 2113, Japan
    Proc Natl Acad Sci U S A 103:16672-6. 2006
    ....
  19. ncbi Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme
    Hanspeter Lauble
    Institut fur Organische Chemie, Universitat Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    Biochemistry 41:12043-50. 2002
    ..The implications of this structure on the evolution of catalysis in the hydroxynitrile lyase superfamily are discussed...
  20. pmc The active site of hydroxynitrile lyase from Prunus amygdalus: modeling studies provide new insights into the mechanism of cyanogenesis
    Ingrid Dreveny
    Institut fur Chemie, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Protein Sci 11:292-300. 2002
    ....
  21. ncbi A bacterial selection for the directed evolution of pyruvate aldolases
    Jennifer S Griffiths
    Department of Chemistry, Duke University, Box 90317, Durham, NC 27708, USA
    Bioorg Med Chem 12:4067-74. 2004
    ..This selection system allows rapid screening of mutant enzyme libraries and facilitates the discovery of enzymes with novel substrate specificities...
  22. ncbi Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes
    Kiyoharu Ito
    Laboratory of Biomembrane and Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Biochem Biophys Res Commun 357:212-7. 2007
    ..The absence of both S1P-degrading enzymes has not been reported for other cell types. Thus, erythrocytes may be specialized cells for storing and supplying plasma S1P...
  23. ncbi Point-counterpoint of sphingosine 1-phosphate metabolism
    Julie D Saba
    Children s Hospital of Oakland Research Institute, Oakland, Calif, USA
    Circ Res 94:724-34. 2004
    ....
  24. ncbi Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis
    Ulrike Reiss
    Children s Hospital Oakland Research Institute, Oakland, California 94609 1673, USA
    J Biol Chem 279:1281-90. 2004
    ....
  25. pmc Sphingosine-1-phosphate lyase potentiates apoptosis via p53- and p38-dependent pathways and is down-regulated in colon cancer
    Babak Oskouian
    Children s Hospital Oakland Research Institute Center for Cancer Research, Oakland, CA 94609 1673, USA
    Proc Natl Acad Sci U S A 103:17384-9. 2006
    ..Our findings suggest that genetic or epigenetic changes affecting intestinal S1P metabolism may correlate with and potentially contribute to carcinogenesis...
  26. ncbi Fatty acid 9- and 13-hydroperoxide lyases from cucumber
    K Matsui
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753 8515, Japan
    FEBS Lett 481:183-8. 2000
    ..By extensive product analyses, it was determined that the recombinant C15 enzyme has only HPL activity and no AOS activity, in spite of its higher sequence similarity to AOS...
  27. ncbi Silencing the jasmonate cascade: induced plant defenses and insect populations
    Andre Kessler
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, Jena 07745, Germany
    Science 305:665-8. 2004
    ....
  28. ncbi Carving the active site of almond R-HNL for increased enantioselectivity
    Roland Weis
    Research Centre Applied Biocatalysis GmbH, Petersgasse 14, 8010 Graz, Austria
    Angew Chem Int Ed Engl 44:4700-4. 2005
  29. ncbi Characterization of three genes encoding enzymes of the folate biosynthetic pathway in Plasmodium falciparum
    C S Lee
    Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology UMIST, UK
    Parasitology 122:1-13. 2001
    ....
  30. pmc Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin
    H Lauble
    Institut für Organische Chemie der Universität Stuttgart, D 70569 Stuttgart, Germany
    Protein Sci 10:1015-22. 2001
    ..The His236 imidazolium cation then facilitates the leaving of the nitrile group by proton donating...
  31. ncbi Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry
    Thomas Purkarthofer
    Research Centre Applied Biocatalysis, Petersgasse 14, 8010 Graz, Austria
    Appl Microbiol Biotechnol 76:309-20. 2007
    ..Recently, protein engineering in combination with in silico methods gave rise to the development of a tailor-made HNL for large-scale manufacturing of a specific target cyanohydrin...
  32. ncbi Reaction mechanism of hydroxynitrile lyases of the alpha/beta-hydrolase superfamily: the three-dimensional structure of the transient enzyme-substrate complex certifies the crucial role of LYS236
    Karl Gruber
    Institut fur Chemie, Physikalische Chemie, Karl Franzens Universitaät Heinrichstrasse 28, A 8010 Graz, Austria
    J Biol Chem 279:20501-10. 2004
    ..Together with a threonine side chain, the active-site serine and lysine are also involved in substrate binding...
  33. ncbi Subcellular localization and membrane topology of sphingosine-1-phosphate lyase in rat liver
    P P Van Veldhoven
    Katolieke Universiteit Leuven, Faculty of Medicine, Department of Pharmacology, Belgium
    J Biol Chem 266:12502-7. 1991
    ..Treatment of intact microsomal vesicles with trypsin or thermolysin inactivated the lyase activity, confirming that its catalytic site(s) or other domains essential for activity face the cytosol...
  34. ncbi The synthesis of chiral cyanohydrins by oxynitrilases
    H Griengl
    Institute of Organic Chemistry, Technical University Graz, SFB Biocatalysis of the Austrian Science Fund, Stremayrgasse 16, A 8010 Graz, Austria
    Trends Biotechnol 18:252-6. 2000
    ..Sufficient quantities of enzyme are available via cheap natural sources and there have been recent advances in overexpression production of cyanohydrins on an industrial scale...
  35. ncbi Structure-based mutagenesis approaches toward expanding the substrate specificity of D-2-deoxyribose-5-phosphate aldolase
    Grace DeSantis
    Department of Chemistry, The Scripps Research Institute, 10550N Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 11:43-52. 2003
    ..coli strain SELECT (Deltaace, adhC, DE3) was developed to be used in the future for selection of DERA variants with novel nonphosphorylated acceptor specificity...
  36. ncbi The hydroxynitrile lyase from almond: a lyase that looks like an oxidoreductase
    I Dreveny
    , , Heinrichstrasse 28, Graz A-8010, Austria
    Structure 9:803-15. 2001
    ..e., a general base and a positive charge to stabilize the cyanide, are not obviously present in the putative active site of PaHNL1. Therefore, the reason for its HNL activity is far from being well understood at this point...
  37. ncbi A cDNA encoding 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate synthase of Pisum sativum L. (pea) functionally complements a kdsA mutant of the Gram-negative bacterium Salmonella enterica
    W Brabetz
    Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany
    Planta 212:136-43. 2000
    ..Thus, plant cells possess a specific enzyme for the biosynthesis of Kdo-8-P with remarkable structural and functional similarities to bacterial KdsA proteins...
  38. ncbi Arabidopsis 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate synthase: cDNA cloning and expression analyses
    Keiichi Matsuura
    Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606 8502, Japan
    J Exp Bot 54:1785-7. 2003
    ....
  39. pmc The gene expression and enzyme activity of plant 3-deoxy-D-manno-2-octulosonic acid-8-phosphate synthase are preferentially associated with cell division in a cell cycle-dependent manner
    Frédéric Delmas
    Unité Mixte de Recherche 619 de Physiologie et Biotechnologie Végétales, Institut de Biologie Végétale Moléculaire, Centre de Recherche Institut National de la Recherche Agronomique Bordeaux, boite postale 81, 33883 Villenave d Ornon Cedex, France
    Plant Physiol 133:348-60. 2003
    ..Furthermore, the transcription of the kdsA gene was found to oscillate during the cell cycle in tobacco (Nicotiana tabacum) Bright-Yellow 2 synchronized cells with a maximum during mitosis...
  40. ncbi Creation of a shikimate pathway variant
    Ningqing Ran
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
    J Am Chem Soc 126:6856-7. 2004
    ..coli constructs that lack phosphoenolpruvate-based DAHP synthase activity and rely on evolved KDPGal aldolase for biosynthesis of shikimate pathway intermediates and products...
  41. ncbi Hydroxynitrile lyases in stereoselective catalysis
    F Effenberger
    Institut für Organische Chemie der Universität Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    Curr Opin Biotechnol 11:532-9. 2000
    ..The enantioselectivity of chiral metal-complex-catalyzed additions of trimethylsilyl cyanide to aldehydes has been improved, but is, by far, not yet competitive with the HNL-catalyzed reactions...
  42. ncbi Cross-linked aggregates of (R)-oxynitrilase: a stable, recyclable biocatalyst for enantioselective hydrocyanation
    Luuk M van Langen
    Laboratory of Biocatalysis and Organic Chemistry, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands
    Org Lett 7:327-9. 2005
    ..The beneficial effect of these latter conditions on the hydrocyanation of slow-reacting aldehydes is demonstrated. The oxynitrilase CLEA was recycled 10 times without loss of activity...
  43. ncbi Study of the (S)-hydroxynitrile lyase from Hevea brasiliensis: mechanistic implications
    U Hanefeld
    Kluyver Laboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Biochim Biophys Acta 1432:185-93. 1999
    ..Therefore the earlier postulated reaction intermediate as well as the proposed action of the catalytic triad have to be reevaluated. The calculated molecular mass could be confirmed by mass spectroscopy...
  44. ncbi CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle association, and proposed new rules for the classification of CYP74 enzymes
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem Soc Trans 34:1223-7. 2006
    ....
  45. ncbi Oxynitrilases for asymmetric C-C bond formation
    D V Johnson
    Spezialforschungsbereich Biokatalyse, , Graz, A 8010, Austria
    Curr Opin Chem Biol 4:103-9. 2000
    ..Advances made in molecular cloning and genetics have delivered information on the oxynitrilase mechanism of action and sufficient quantities of enzyme to satisfy industrial requirements...
  46. ncbi The BST1 gene of Saccharomyces cerevisiae is the sphingosine-1-phosphate lyase
    J D Saba
    Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 272:26087-90. 1997
    ..This study confirms that sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism...
  47. pmc Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem J 395:641-52. 2006
    ..Highly concentrated and monodispersed samples of detergent-free CYP74C3 protein may be well suited for the purposes of crystallization and structural resolution of the first plant cytochrome P450 enzyme...
  48. ncbi How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications
    J N Andexer
    Institut für Molekulare Enzymtechnologie, Universitat Dusseldorf, D 52426 Jülich, Germany
    Trends Biotechnol 27:599-607. 2009
    ..Particular emphasis is placed on the necessity to combine approaches from different fields, such as enzyme engineering and reaction engineering...
  49. ncbi Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli
    Dean Rea
    Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK
    J Mol Biol 373:866-76. 2007
    ..6 A and 2.0 A, respectively. Comparison with similar structures of the homologous 2-dehydro-3-deoxygalactarate aldolase, coupled with site-directed mutagenesis data, implicate histidine 45 and arginine 70 as key catalytic residues...
  50. ncbi Observation of a short, strong hydrogen bond in the active site of hydroxynitrile lyase from Hevea brasiliensis explains a large pKa shift of the catalytic base induced by the reaction intermediate
    Gudrun R Stranzl
    Institut fur Chemie, Physikalische Chemie, Karl Franzens Universität Heinrichstrasse 28, A 8010 Graz, Austria
    J Biol Chem 279:3699-707. 2004
    ..6 kcal/mol, equivalent to a shift in the apparent pK(a) of His(235) from 2.5 to 10. This huge inhibitor-induced increase in basicity is a prerequisite for His(235) to act as general base in the HbHNL-catalyzed cyanohydrin reaction...
  51. ncbi [So-called "green odor" as plant origin--chemistry and biochemistry]
    Akikazu Hatanaka
    Library of Yamaguchi University, 1677 1 Yoshida, Yamaguchi 753 8516, Japan
    Seikagaku 75:1414-28. 2003
  52. ncbi Probing the role of metal ions in the catalysis of Helicobacter pylori 3-deoxy-D-manno-octulosonate-8-phosphate synthase using a transient kinetic analysis
    Apurba Kumar Sau
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 279:15787-94. 2004
    ....
  53. pmc Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-d-ribose-5-phosphate aldolase
    Haruhiko Sakuraba
    Department of Life System, Institute of Technology and Science, University of Tokushima, 2 1 Minamijosanjima cho, Tokushima 770 8506, Japan
    Appl Environ Microbiol 73:7427-34. 2007
    ..This promotes the formation of the unique dimeric structure and strengthens the hydrophobic intersubunit interactions. These structural features are considered responsible for the extremely high stability of the hyperthermophilic DERAs...
  54. ncbi Structure and catalytic mechanism of L-rhamnulose-1-phosphate aldolase
    Markus Kroemer
    Institut fur Organische Chemie und Biochemie, Albertstrasse 21, 79104 Freiburg im Breisgau, Germany
    Biochemistry 42:10560-8. 2003
    ..We suggest that this movement supports the catalysis mechanically...
  55. ncbi Cloning and characterization of a gene encoding phosphoketolase in a Lactobacillus paraplantarum isolated from Kimchi
    Do Won Jeong
    Department of Agricultural Biotechnology, Seoul National University, Seoul 151 742, Korea
    J Microbiol Biotechnol 17:822-9. 2007
    ..08 +/- 0.057 mM (mean +/- SD) and 499.21 +/- 4.33 micromol/min/mg, respectively, and the optimum temperature and pH for the production of acetyl phosphate were 45 degrees C and 7.0, respectively...
  56. pmc Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates
    Manoj Cheriyan
    Department of Chemistry, University of Michigan, Ann Arbor 48109, USA
    Protein Sci 16:2368-77. 2007
    ..Furthermore, the S184L KDPG aldolase mutant improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40-fold, making it a useful biocatalyst for the preparation of fine chemicals...
  57. pmc Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl ester
    Gabriel S Brandt
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 47:7734-43. 2008
    ..We also delineated the role of His29 (the sole potential acid-base catalyst in the active site other than the highly conserved Glu50) and Trp163 in cofactor activation and catalysis by benzaldehyde lyase...
  58. ncbi Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values
    Jan von Langermann
    Institut fur Chemie, Universitat Rostock, Albert Einstein Str 3A, 18059 Rostock, Germany
    Bioprocess Biosyst Eng 31:155-61. 2008
    ..e.(S)). Additionally, a new continuous spectroscopic cyanohydrin assay monitoring the formation of 3-phenoxy-benzaldehyde cyanohydrin was developed...
  59. ncbi Mechanistic insight into 3-deoxy-D-manno-octulosonate-8-phosphate synthase and 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase utilizing phosphorylated monosaccharide analogues
    David L Howe
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 42:4843-54. 2003
    ..coli KDO8-P synthase...
  60. ncbi Antenna domain mobility and enzymatic reaction of L-rhamnulose-1-phosphate aldolase
    Dirk Grueninger
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstrasse 21, 79104 Freiburg im Breisgau, Germany
    Biochemistry 47:607-14. 2008
    ..We suggest that, like K15W, the other mutations also followed the design, validating the correlation between antenna mobility and activity. This correlation suggests that the domain mobility facilitates the reaction...
  61. ncbi Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase
    Henry J Lamble
    Centre for Extremophile Research, the Department of Biology and Biochemistry, University of Bath, United Kingdom
    J Biol Chem 278:34066-72. 2003
    ..The physiological and evolutionary implications of this observation are discussed in terms of catalytic and metabolic promiscuity...
  62. ncbi Structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida
    B J Bell
    Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA
    Acta Crystallogr D Biol Crystallogr 59:1454-8. 2003
    ..Except for the N-terminal helix, the structure of KDPG aldolase from P. putida closely resembles the structure of the homologous enzyme from Escherichia coli...
  63. ncbi Cloning, expression, and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus
    Smadar Shulami
    Department of Food Engineering and Biotechnology, Technion Israel Institute of Technology, 32000 Haifa, Israel
    Extremophiles 7:471-81. 2003
    ..These results indicate that A. pyrophilus KDO8P synthase is a metal-dependent enzyme. A C11A mutant of KDO8P synthase from A. pyrophulis retained less than 1% of the wild-type activity and was shown to be incapable of metal binding...
  64. ncbi Mechanism of dihydroneopterin aldolase. NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes
    Yi Wang
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    FEBS J 274:2240-52. 2007
    ..The results show that the two enzymes have significantly different binding and catalytic properties, in accordance with the significant sequence differences between them...
  65. ncbi Structural and mechanistic changes along an engineered path from metallo to nonmetallo 3-deoxy-D-manno-octulosonate 8-phosphate synthases
    Fathima Kona
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 46:4532-44. 2007
    ..These differences may reflect an evolutionary adaptation of metallo and nonmetallo KDO8PS's to the cellular concentrations of these metabolites in their respective hosts...
  66. ncbi Kinetics of barley FA hydroperoxide lyase are modulated by salts and detergents
    Takao Koeduka
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yoshida 1677 1, Yamaguchi, 753 8515, Japan
    Lipids 38:1167-72. 2003
    ..These results suggest that these effectors induced a conformational change in barley HPL, resulting in an improvement in substrate binding and in enzyme activity...
  67. pmc Bacillus subtilis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase revisited: resolution of two long-standing enigmas
    Jing Wu
    Department of Medicinal Chemistry and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1065, USA
    Biochem J 390:583-90. 2005
    ..6+/-0.1 s(-1) for DAHPS activity and K(m)(chorismate)=850+/-97 microM, kcat=0.41+/-0.01 s(-1) for CM activity. B. subtilis DAHPS is inhibited by the Shikimate pathway intermediates prephenate and chorismate...
  68. ncbi The use of (E)- and (Z)-phosphoenol-3-fluoropyruvate as mechanistic probes reveals significant differences between the active sites of KDO8P and DAHP synthases
    Cristina M Furdui
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
    Biochemistry 44:7326-35. 2005
    ..1 s(-)(1) for (Z)-FPEP, respectively]. The observed data indicate substantial differences in the PEP subsites and open the opportunity for the design of selective inhibitors against these two families of enzymes...
  69. ncbi A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli
    Smadar Shulami
    Department of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa, Israel
    J Biol Chem 279:45110-20. 2004
    ....
  70. ncbi Thermodynamic properties of nucleotide reductase reactions
    Robert A Alberty
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 43:9840-5. 2004
    ..15 K. The apparent equilibrium constant for ADP + thioredoxin(red) = 2'-deoxyADP + H(2)O + thioredoxin(ox) is 1.4 x 10(11) at 298.15 K, pH 7, and 0.25 M ionic strength...
  71. ncbi Crystal structures of Escherichia coli KDO8P synthase complexes reveal the source of catalytic irreversibility
    Radion Vainer
    Department of Chemistry and Institute of Catalysis, Science and Technology, Technion Israel Institute of Technology, Technion City, Haifa 32000, Israel
    J Mol Biol 351:641-52. 2005
    ....
  72. ncbi Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: an ancestral 3-deoxyald-2-ulosonate-phosphate synthase?
    Linley R Schofield
    Institutes of Fundamental Sciences and Molecular BioSciences, Massey University, Palmerston North, New Zealand
    Biochemistry 44:11950-62. 2005
    ..furiosus DAH7PS may be the contemporary protein closest to the ancestral type I enzyme...
  73. ncbi Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions
    Meekyung Ahn
    Institute of Fundamental Sciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand
    Org Biomol Chem 3:4046-9. 2005
    ....
  74. ncbi Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase
    Thomas B Thompson
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 279:37956-63. 2004
    ..Together, the structure and functional analysis provide insight into the structural framework necessary to perform the complex rearrangements catalyzed by this class of polyketide cyclases...
  75. ncbi Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens
    Tanja G Mosbacher
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    FEBS J 272:6067-76. 2005
    ..Due to its substrate spectrum and stereospecificity, the enzyme extends the synthetic potential for carboligations appreciably...
  76. ncbi Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus
    Henry J Lamble
    Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    FEBS Lett 579:6865-9. 2005
    ..solfataricus is also promiscuous for the metabolism of both glucose and galactose...
  77. pmc Mechanism of the Class I KDPG aldolase
    Stephen W B Fullerton
    Centre for Biomolecular Sciences, The University of St Andrews, St Andrews, KY16 9ST, UK
    Bioorg Med Chem 14:3002-10. 2006
    ..Many aspects of this mechanism are observed in other Class I aldolases and suggest a mechanistically and, perhaps, evolutionarily related family of aldolases distinct from the N-acetylneuraminate lyase (NAL) family...
  78. ncbi Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism
    Verena König
    Department of Structural Chemistry, University of Gottingen, Tammannstr 4, D 37077 Gottingen, Germany
    J Mol Biol 337:675-90. 2004
    ....
  79. ncbi Hydroperoxide lyases (CYP74C and CYP74B) catalyze the homolytic isomerization of fatty acid hydroperoxides into hemiacetals
    Alexander N Grechkin
    Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P O Box 30, Kazan, 420111 Russia
    Biochim Biophys Acta 1761:1419-28. 2006
    ....
  80. ncbi Formation of volatile compounds in model experiments with crude leek (Allium ampeloprasum Var. Lancelot) enzyme extract and linoleic acid or linolenic acid
    Ghita Studsgaard Nielsen
    Department of Food Science and LMC, Centre of Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, 1958 Frederiksberg C, Denmark
    J Agric Food Chem 52:2315-21. 2004
    ..Leek LOX produced only the 13-hydroperoxide of linoleic acid and linolenic acid...
  81. pmc Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases
    Suzanne Wolterink-van Loo
    Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands
    Biochem J 403:421-30. 2007
    ..Water-mediated interactions permit binding of substrates in multiple conformations in the spacious hydrophilic binding site, and correlate with the observed broad substrate specificity...
  82. ncbi Structure of aldolase from Thermus thermophilus HB8 showing the contribution of oligomeric state to thermostability
    Neratur K Lokanath
    Highthroughput Factory, RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
    Acta Crystallogr D Biol Crystallogr 60:1816-23. 2004
    ..From these results, it is concluded that the oligomeric state of 2-deoxyribose-5-phosphate aldolase is important for the thermostability and not for the catalytic function...
  83. ncbi Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase
    Jian Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Mol Biol 324:205-14. 2002
    ..aeolicus KDO8PS is to orient PEP in the active site of the enzyme in such a way that a water molecule on the sinister (si) side of PEP can be activated by direct coordination to the metal ion...
  84. pmc De novo computational design of retro-aldol enzymes
    Lin Jiang
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Science 319:1387-91. 2008
    ..The atomic accuracy of the design process was confirmed by the x-ray crystal structure of active designs embedded in two protein scaffolds, both of which were nearly superimposable on the design model...
  85. ncbi Observation of covalent intermediates in an enzyme mechanism at atomic resolution
    A Heine
    Department of Molecular Biology, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 294:369-74. 2001
    ..A proton relay system appears to activate a conserved active-site water that functions as the critical mediator for proton transfer...
  86. ncbi Crystal structures of KDOP synthase in its binary complexes with the substrate phosphoenolpyruvate and with a mechanism-based inhibitor
    O Asojo
    Department of Chemistry, University of Houston, Houston, Texas 77204 5641, USA
    Biochemistry 40:6326-34. 2001
    ....
  87. ncbi Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli
    N Wymer
    Department of Chemistry, LSRC, Duke University, Durham, NC 27708, USA
    Structure 9:1-9. 2001
    ..To date such approaches have furnished novel activities through multiple mutations of residues involved in recognition; in no instance has a key catalytic residue been altered while activity is retained...
  88. pmc Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution
    J Allard
    Departement de Biochimie, Universite de Montreal, Montreal, QC, H3C 3J7 Canada
    Proc Natl Acad Sci U S A 98:3679-84. 2001
    ..Formation of the zwitterionic pair requires prior side chain rearrangement by protonated Lys-133 to displace a water molecule, hydrogen bonded to the zwitterionic residues...
  89. ncbi Purification, substrate specificity and binding, -decarboxylase activity, and other properties of Escherichia coli 2-keto-4-hydroxyglutarate aldolase
    H Nishihara
    J Biol Chem 247:5079-87. 1972
  90. ncbi The three-dimensional structure of N-acetylneuraminate lyase from Escherichia coli
    T Izard
    Biomolecular Research Institute, Parkville, Australia
    Structure 2:361-9. 1994
    ..The enzyme plays an important role in the regulation of sialic acid metabolism in bacteria. The reverse reaction can be exploited for the synthesis of sialic acid and some of its derivatives...
  91. ncbi Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis
    A C Joerger
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, D 79104 Freiburg im Breisgau, Germany
    Biochemistry 39:6033-41. 2000
    ..The effect of some mutations on enantioselectivity and on the ratio of diastereomer formation indicates clearly the binding site of the aldehyde moiety in relation to the other substrate dihydroxyacetone phosphate...
  92. ncbi Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase
    S Radaev
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 275:9476-84. 2000
    ..This observation suggests that KDO8P synthase and DAH7P synthase evolved from a common ancestor and that they adopt the same catalytic strategy...
  93. ncbi Catalytic mechanism of Kdo8P synthase: transient kinetic studies and evaluation of a putative reaction intermediate
    P H Liang
    Department of Chemistry, Technion Israel Institute of Technology, Haifa, Israel
    Biochemistry 37:16390-9. 1998
    ..and Abeles, R. H. (1988) Biochem. Biophys. Res. Commun. 157, 816-820], which suggests a reaction pathway involving an acyclic bisphosphate intermediate 1...
  94. ncbi The structure of L-ribulose-5-phosphate 4-epimerase: an aldolase-like platform for epimerization
    Y Luo
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Biochemistry 40:14763-71. 2001
    ..Detailed inspection of the epimerase structure, however, indicates that despite the close overall structural similarity to class II aldolases, the enzyme has evolved distinct active site features that promote its particular chemistry...
  95. ncbi Two class II D-tagatose-bisphosphate aldolases from enteric bacteria
    Andreas Brinkkötter
    Universitat Osnabruck, Fachbereich Biologie, 49069 Osnabruck, Germany
    Arch Microbiol 177:410-9. 2002
    ..The Z subunits alone did not show any aldolase activity. Close relatives of these new TagBP aldolases were found in several gram-negative and gram-positive bacteria, e.g., Streptomyces coelicolor...
  96. ncbi Structures of l-fuculose-1-phosphate aldolase mutants outlining motions during catalysis
    A C Joerger
    Institut fur Organische Chemie und Biochemie, Freiburg im Breisgau, Germany 79104, USA
    J Mol Biol 303:531-43. 2000
    ..This observation explains our failure to find mutants that accept phosphate-free substitutes for DHAP. The FucA zinc coordination sphere is compared with that of carbonic anhydrase...
  97. ncbi Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis
    J Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 40:15676-83. 2001
    ..Overall, the process can be described as a syn addition of water and A5P to the si side of PEP...
  98. pmc Phospholipase A2 activity triggers the wound-activated chemical defense in the diatom Thalassiosira rotula
    Georg Pohnert
    Max Planck Institut für Chemische Okologie, Winzerlaer Strasse 10, D 07745 Jena, Germany
    Plant Physiol 129:103-11. 2002
    ....
  99. ncbi Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase
    Jennifer S Griffiths
    Department of Chemistry, Duke University, Durham, NC 27708, USA
    Bioorg Med Chem 10:545-50. 2002
    ..A preparative scale reaction between 2-pyridine carboxaldehyde and pyruvate provided product of the same absolute configuration as mesophilic enzymes, but with diminished stereoselectivity...
  100. ncbi Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis
    Emine Mattern-Dogru
    Lehrstuhl fur Pharmazeutische Biologie, Institut fur Pharmazie, Johannes Gutenberg Universitat Mainz, Germany
    Eur J Biochem 269:2889-96. 2002
    ..The results showed that PNAE is a novel member of the alpha/beta hydrolase enzyme superfamily...
  101. ncbi Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase
    Minke A Noordermeer
    Bijvoet Center for Biomolecular Research, Department of Bio Organic Chemistry, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Agric Food Chem 50:4270-4. 2002
    ..Recombinant hydroperoxide lyase is stable and can be stored at 4 degrees C for 1 month without significant loss of activity...

Research Grants26

  1. ENZYME CATALYZED SYNTHESIS OF MONOSACCHARIDES
    George Whitesides; Fiscal Year: 2000
    ..Provide reagents and non-enzymatic reactions needed to support these efforts in enzyme-catalyzed synthesis. ..
  2. ALDOLASE GENES IN NORMAL AND FRUCTOSE INTOLERANT HUMANS
    DEAN TOLAN; Fiscal Year: 1991
    ..Finally, competition experiments performed in vivo will investigate the elements and modes of trans-regulatory control involved in this family of genes...
  3. METALLOBIOCHEMISTRY OF VANADIUM(V)
    DEBBIE CRANS; Fiscal Year: 1993
    ..Increased understanding of how vanadium acts may eventually result in vanadate derivatives that could be used biomedically...
  4. ENZYMES IN ORGANIC SYNTHESIS
    Chi Huey Wong; Fiscal Year: 1999
    ..5. Provide other enzymatic and non-enzymatic reactions, NMR and modeling techniques to support these efforts in synthesis and molecular design. ..
  5. NOVEL DHAP-DEPENDENT ALDOLASES FOR ORGANIC SYNTHESIS
    FRANCISCO MORIS VARAS; Fiscal Year: 2000
    ..Aldolases will help syntheses or pharmaceutical drugs or intermediates efficiently and economically and reduce the chemical waste generation. ..
  6. The Evaluation of Synthetically Useful Aldolases
    Eric Toone; Fiscal Year: 2005
    ..Together, these studies will significantly advance the goal of rational catalyst/biocatalyst design/redesign and enhance the synthetic utility of the pyruvate aldolases. ..
  7. MICROBIAL GLYCOLYSIS MUTANTS
    DAN FRAENKEL; Fiscal Year: 1992
    ..These include experiments on rate limitation in the pathway and on transit time...
  8. Sphingolipid signaling in olfactory mucosa
    Mary Beth Genter; Fiscal Year: 2004
    ..abstract_text> ..
  9. Strategies for Selective Covalent Conjugation of Proteins with Small Molecules
    Fujie Tanaka; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  10. Universal Technology for Profiling the Dynamics of Normal & Oncogenic Signaling
    Karen Anderson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  11. NAVBO Workshop: Biology of Signaling in the Cardiovascular System
    Timothy Hla; Fiscal Year: 2008
    ..End of Abstract) [unreadable] [unreadable] [unreadable]..
  12. Pathogenesis of a Novel Limb-Girdle Muscular Dystrophy
    Michio Hirano; Fiscal Year: 2003
    ..For the patients, achieving the proposed goals will allow more accurate prenatal diagnosis, genetic counseling, and perhaps contribute to more rational therapies in the future. ..
  13. Extracellular matrix in chloracetanilide carcinogenesis
    Mary Beth Genter; Fiscal Year: 2004
    ..abstract_text> ..
  14. MECHANISM OF INDUCTION OF OLFACTORY TUMORS BY ALACHLOR
    Mary Beth Genter; Fiscal Year: 2002
    ....
  15. Selective Antibody Conjugates
    Subhash C Sinha; Fiscal Year: 2010
    ..This strategy has the advantage that only a single antibody is required for a multiplicity of tasks and it taps into the unlimited chemical diversity and the specificity that can be engendered by organic synthesis. ..
  16. Asymmetric Aminocatalysis
    SUBHASH SINHA; Fiscal Year: 2003
    ..2) Designing novel aminocatalysts for aldol, Mannich, and Michael reactions. (3) High-throughput screening of aminocatalytic libraries. (4) Synthesizing biologically active natural products and drugs using aminocatalysis. ..
  17. Anticancer drug resistance studies using Dictyostelium
    Stephen Alexander; Fiscal Year: 2003
    ..These studies will identify new mechanisms for drug resistance and new targets for chemotherapeutic intervention which can subsequently be validated in human cells. ..
  18. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  19. Novel Approach to Study Transient Enzyme Intermediates
    Karen Anderson; Fiscal Year: 2008
    ..These studies will aid in pioneering a powerful and novel analytical methodology for detecting, characterizing and quantitating enzyme intermediates on a rapid time scale. ..
  20. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  21. PATHOPHYSIOLOGICAL ROLE OF 12-R-LIPOXYGENASE
    ALAN BRASH; Fiscal Year: 2002
    ..abstract_text> ..
  22. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2003
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..
  23. NOVEL OXYGENATIONS OF ARACHIDONIC ACID
    ALAN BRASH; Fiscal Year: 2006
    ..These studies will develop new insights into the biochemistry of mammalian cyclooxygenases and as a consequence have practical benefits in development of therapeutics. ..