carbon oxygen lyases

Summary

Summary: Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. EC 4.2.

Top Publications

  1. ncbi Enhancement of the primary flavor compound methional in potato by increasing the level of soluble methionine
    Rong Di
    Biotechnology Center for the Agriculture and the Environment, Department of Plant Biology and Pathology, Rutgers University, Cook College, 59 Dudley Road, New Brunswick, NJ 08901 8520, USA
    J Agric Food Chem 51:5695-702. 2003
  2. ncbi Keynote: past, present, and future aspects of base excision repair
    T Lindahl
    ICRF Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Prog Nucleic Acid Res Mol Biol 68:xvii-xxx. 2001
  3. ncbi BER, MGMT, and MMR in defense against alkylation-induced genotoxicity and apoptosis
    B Kaina
    Division of Applied Toxicology, Institute of Toxicology, University of Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany
    Prog Nucleic Acid Res Mol Biol 68:41-54. 2001
  4. ncbi The HAP1 protein stimulates the turnover of human mismatch-specific thymine-DNA-glycosylase to process 3,N(4)-ethenocytosine residues
    C V Privezentzev
    , UMR 8532 CNRS, 39 rue C. Desmoulins, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Mutat Res 480:277-84. 2001
  5. pmc Highly efficient base excision repair (BER) in human and rat male germ cells
    A K Olsen
    Section for Product Toxicology, Department of Environmental Medicine, National Institute of Public Health, PO Box 4404 Nydalen, N 0403 Oslo, Norway
    Nucleic Acids Res 29:1781-90. 2001
  6. ncbi From famine to feast: the role of methylglyoxal production in Escherichia coli
    S Tötemeyer
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, UK
    Mol Microbiol 27:553-62. 1998
  7. pmc The N-terminal region of Arabidopsis cystathionine gamma-synthase plays an important regulatory role in methionine metabolism
    Yael Hacham
    Plant Science Laboratory, Migal Galilee Technology Center, Rosh Pina 12100, Israel
    Plant Physiol 128:454-62. 2002
  8. pmc Nascent peptide-mediated translation elongation arrest coupled with mRNA degradation in the CGS1 gene of Arabidopsis
    Hitoshi Onouchi
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Genes Dev 19:1799-810. 2005
  9. ncbi Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants
    Golan Amira
    Plant Science Laboratory, Migal Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
    J Exp Bot 56:2443-52. 2005
  10. ncbi An in vivo internal deletion in the N-terminus region of Arabidopsis cystathionine gamma-synthase results in CGS expression that is insensitive to methionine
    Yael Hacham
    Laboratory of Plant Science, Migal Galilee Technology Center, P O Box 831, Kiryat Shmona 12100, Israel
    Plant J 45:955-67. 2006

Detail Information

Publications166 found, 100 shown here

  1. ncbi Enhancement of the primary flavor compound methional in potato by increasing the level of soluble methionine
    Rong Di
    Biotechnology Center for the Agriculture and the Environment, Department of Plant Biology and Pathology, Rutgers University, Cook College, 59 Dudley Road, New Brunswick, NJ 08901 8520, USA
    J Agric Food Chem 51:5695-702. 2003
    ..These results provide the first evidence that the methional level can be enhanced in processed potatoes by increasing the production of its precursor, methionine...
  2. ncbi Keynote: past, present, and future aspects of base excision repair
    T Lindahl
    ICRF Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Prog Nucleic Acid Res Mol Biol 68:xvii-xxx. 2001
    ....
  3. ncbi BER, MGMT, and MMR in defense against alkylation-induced genotoxicity and apoptosis
    B Kaina
    Division of Applied Toxicology, Institute of Toxicology, University of Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany
    Prog Nucleic Acid Res Mol Biol 68:41-54. 2001
    ..Obviously, nonrepaired BER intermediates are clastogenic and act as a strong trigger of the apoptotic pathway. The elements of this pathway are currently under investigation...
  4. ncbi The HAP1 protein stimulates the turnover of human mismatch-specific thymine-DNA-glycosylase to process 3,N(4)-ethenocytosine residues
    C V Privezentzev
    , UMR 8532 CNRS, 39 rue C. Desmoulins, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Mutat Res 480:277-84. 2001
    ..However, using a variety of techniques including gel retardation assay, surface plasmon resonance and two-hybrid system, it was not possible to detect evidence for a complex including the substrate, the hTDG and HAP1 proteins...
  5. pmc Highly efficient base excision repair (BER) in human and rat male germ cells
    A K Olsen
    Section for Product Toxicology, Department of Environmental Medicine, National Institute of Public Health, PO Box 4404 Nydalen, N 0403 Oslo, Norway
    Nucleic Acids Res 29:1781-90. 2001
    ..This efficient BER removes frequently occurring DNA lesions that arise spontaneously or via environmental agents, thereby minimising the number of potential mutations transferred to the next generation...
  6. ncbi From famine to feast: the role of methylglyoxal production in Escherichia coli
    S Tötemeyer
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, UK
    Mol Microbiol 27:553-62. 1998
    ..A mutant lacking MGS was constructed, and it grew normally on a range of carbon sources and on low-phosphate medium. However, the mutant failed to produce MG during growth on xylose in the presence of cAMP, and growth was inhibited...
  7. pmc The N-terminal region of Arabidopsis cystathionine gamma-synthase plays an important regulatory role in methionine metabolism
    Yael Hacham
    Plant Science Laboratory, Migal Galilee Technology Center, Rosh Pina 12100, Israel
    Plant Physiol 128:454-62. 2002
    ....
  8. pmc Nascent peptide-mediated translation elongation arrest coupled with mRNA degradation in the CGS1 gene of Arabidopsis
    Hitoshi Onouchi
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Genes Dev 19:1799-810. 2005
    ..Furthermore, our data suggest that the ribosome stalls at the step of translocation rather than at the step of peptidyl transfer...
  9. ncbi Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants
    Golan Amira
    Plant Science Laboratory, Migal Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
    J Exp Bot 56:2443-52. 2005
    ....
  10. ncbi An in vivo internal deletion in the N-terminus region of Arabidopsis cystathionine gamma-synthase results in CGS expression that is insensitive to methionine
    Yael Hacham
    Laboratory of Plant Science, Migal Galilee Technology Center, P O Box 831, Kiryat Shmona 12100, Israel
    Plant J 45:955-67. 2006
    ....
  11. pmc Constitutive overexpression of cystathionine gamma-synthase in Arabidopsis leads to accumulation of soluble methionine and S-methylmethionine
    Jungsup Kim
    Biotechnology Center for Agriculture and the Environment, Plant Science Department, Rutgers University, New Brunswick, New Jersey 08901 8520, USA
    Plant Physiol 128:95-107. 2002
    ..The results suggest that the abnormalities associated with CGS and SAM synthetase silencing are due in part to a reduced ability to produce SAM and that SAM may be a regulator of CGS expression...
  12. pmc Functional analysis of cystathionine gamma-synthase in genetically engineered potato plants
    Oliver Kreft
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Department of Professor Dr Lothar Willmitzer, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Physiol 131:1843-54. 2003
    ..These results are inconsistent with the current hypothesis that CgS exerts major Met metabolic flux control in higher plants...
  13. pmc S-adenosyl-L-methionine is an effector in the posttranscriptional autoregulation of the cystathionine gamma-synthase gene in Arabidopsis
    Yukako Chiba
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Proc Natl Acad Sci U S A 100:10225-30. 2003
    ..Together, the results show that the basic reactions of CGS1 exon 1-mediated posttranscriptional regulation occur in the wheat germ in vitro translation system, and that AdoMet acts as the effector...
  14. pmc Antisense inhibition of threonine synthase leads to high methionine content in transgenic potato plants
    M Zeh
    , , 14476 Golm, Germany
    Plant Physiol 127:792-802. 2001
    ..Tubers of TS antisense potato plants contain a Met level increased by a factor of 30 and no reduction in Thr. These plants offer a major biotechnological advance toward the development of crop plants with improved nutritional quality...
  15. ncbi Methylglyoxal bypass identified as source of chiral contamination in l(+) and d(-)-lactate fermentations by recombinant Escherichia coli
    T B Grabar
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
    Biotechnol Lett 28:1527-35. 2006
    ..In mineral salts medium containing 1 mM betaine, both strains produced over 115 g (1.3 mol) lactate from 12% (w/v) glucose, >95% theoretical yield...
  16. ncbi Phototriggered formation and repair of DNA containing a site-specific single strand break of the type produced by ionizing radiation or AP lyase activity
    K Zhang
    Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA
    Biochemistry 40:153-9. 2001
    ....
  17. ncbi Contribution of E. coli AlkA, TagA glycosylases and UvrABC-excinuclease in MMS mutagenesis
    E Grzesiuk
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul Pawinskiego 5a, 02 106 Warszawa, Poland
    Mutat Res 480:77-84. 2001
    ..In the absence of only TagA and AlkA glycosylases, UvrABC excinuclease mediates activation of the SOS response, and this results in an increase of mutagenesis induced by the presence of alkylated bases in DNA...
  18. pmc Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylases
    Erik J Spek
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 184:3501-7. 2002
    ..These studies shed light on the underlying mechanism of NO*-induced homologous recombination...
  19. ncbi Alternative nucleotide incision repair pathway for oxidative DNA damage
    Alexander A Ischenko
    Groupe Réparation de l ADN, UMR 8532 CNRS, LBPA ENS Cachan, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Nature 415:183-7. 2002
    ..The 3'-hydroxyl terminus provides the proper end for DNA repair synthesis; the dangling damaged nucleotide on the 5' side is then a good substrate for human flap-structure endonuclease and for DNA polymerase I of E. coli...
  20. pmc Repair of chromosomal abasic sites in vivo involves at least three different repair pathways
    M Otterlei
    Institute of Cancer Research and Molecular Biology, The Faculty of Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    EMBO J 19:5542-51. 2000
    ..Spontaneous mutation spectra were generally strongly dependent upon the repair background. In conclusion, BER, recombination and TLS all contribute to the handling of chromosomal AP sites in E.coli in vivo...
  21. ncbi Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates
    Mary Ann Pope
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84103, USA
    J Biol Chem 277:22605-15. 2002
    ..These results constitute the first evidence of an interaction between the MutY-product DNA complex and Endo IV or Exo III. Furthermore, they suggest a role for the C-terminal domain of MutY in mediating this interaction...
  22. ncbi Arabidopsis UVH3 gene is a homolog of the Saccharomyces cerevisiae RAD2 and human XPG DNA repair genes
    Z Liu
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    Plant J 26:329-38. 2001
    ..Finally, we show that the AtRAD2/XPG gene is expressed at moderate levels in all plant tissues...
  23. pmc A novel fluorometric oligonucleotide assay to measure O( 6)-methylguanine DNA methyltransferase, methylpurine DNA glycosylase, 8-oxoguanine DNA glycosylase and abasic endonuclease activities: DNA repair status in human breast carcinoma cells overexpressin
    E L Kreklau
    Department of Pharmacology, Indiana University Cancer Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Nucleic Acids Res 29:2558-66. 2001
    ....
  24. ncbi Human endonuclease III acts preferentially on DNA damage opposite guanine residues in DNA
    L Eide
    Department of Molecular Biology, Institute of Medical Microbiology, National Hospital of Norway, 0027 Oslo, Norway
    Biochemistry 40:6653-9. 2001
    ....
  25. pmc Enhanced activity of adenine-DNA glycosylase (Myh) by apurinic/apyrimidinic endonuclease (Ape1) in mammalian base excision repair of an A/GO mismatch
    H Yang
    Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA and Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Nucleic Acids Res 29:743-52. 2001
    ..Consequently, Ape1 preserves the Myh preference for A/GO over A/G and improves overall glycosylase efficiency. Our study suggests that protein-protein interactions may occur in vivo to achieve efficient BER of A/GO...
  26. pmc Reconstitution of the base excision repair pathway for 7,8-dihydro-8-oxoguanine with purified human proteins
    B Pascucci
    Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanita, Viale Regina Elena 299, I 00161 Rome, Italy
    Nucleic Acids Res 30:2124-30. 2002
    ..Thus, the assembly of all the core proteins for 8-oxoG repair catalyses one major pathway that involves single nucleotide repair patches...
  27. pmc Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step
    A E Vidal
    , , UMR217 CNRS-CEA, BP6, F92265 Fontenay aux Roses, France
    Nucleic Acids Res 29:1285-92. 2001
    ..These data indicate that, in the presence of HAP1, the BER of 8-oxoG residues can be highly efficient by bypassing the AP lyase activity of hOGG1 and thus excluding a potentially rate limiting step...
  28. pmc Recombinational repair is critical for survival of Escherichia coli exposed to nitric oxide
    E J Spek
    Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 183:131-8. 2001
    ..can induce. Furthermore, the importance of recombinational repair for cellular survival of NO(.) exposure reveals a potential susceptibility factor for invading microbes...
  29. pmc Stimulation of human 8-oxoguanine-DNA glycosylase by AP-endonuclease: potential coordination of the initial steps in base excision repair
    J W Hill
    Sealy Center for Molecular Science, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
    Nucleic Acids Res 29:430-8. 2001
    ..These results suggest coordinated functions of OGG1 and APE1, and possibly other enzymes, in the DNA base excision repair pathway...
  30. pmc Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress
    Robert W Sobol
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 99:6860-5. 2002
    ..These results illustrate that BER plays a role in protecting mouse embryonic fibroblast cells against methylation-induced mutations and characterize the effect of a particular combination of BER gene defect and environmental exposure...
  31. ncbi Yeast ribosomal protein S3 has an endonuclease activity on AP DNA
    S O Jung
    Laboratory of Biochemistry, Graduate School of Biotechnology and BioInstitute, Korea University, Seoul
    Mol Cells 12:84-90. 2001
    ..From these results, both the mammalian rpS3 and Rps3p appear to be involved in DNA damage processing, but in different modes...
  32. ncbi Zinc binding and redox control of p53 structure and function
    P Hainaut
    Group of Molecular Carcinogenesis, International Agency for Research on Cancer, Lyon, France
    Antioxid Redox Signal 3:611-23. 2001
    ..This redox sensitivity may be one of the biochemical mechanisms by which p53 acts as a "sensor" of multiple forms of stress...
  33. ncbi The OGG1 gene encodes a repair enzyme for oxidatively damaged DNA and is involved in human carcinogenesis
    K Shinmura
    Biology Division, National Cancer Center Research Institute, Tokyo, Japan
    Antioxid Redox Signal 3:597-609. 2001
    ..Repair activities of some mutated and polymorphic OGG1 proteins are lower than those of wild-type OGG1-type la-Ser326 protein and, thus, could be involved in human carcinogenesis...
  34. ncbi Repair of 8-oxoguanine and Ogg1-incised apurinic sites in a CHO cell line
    S Boiteux
    , France
    Prog Nucleic Acid Res Mol Biol 68:95-105. 2001
    ..The results also show that CHO cells efficiently repair DNA molecules that contain an Ogg1-incised AP site, which is the first intermediate in the course of base excision repair of 8-oxoG...
  35. ncbi Potential double-flipping mechanism by E. coli MutY
    P G House
    Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    Prog Nucleic Acid Res Mol Biol 68:349-64. 2001
    ..Molecular modeling techniques employed to integrate the two domains of MutY with DNA suggest that MutY can wrap around the DNA and initiate catalysis by potentially flipping adenine and 8-oxoguanine out of the DNA helix...
  36. ncbi The mechanism of switching among multiple BER pathways
    E Dogliotti
    Laboratory of Comparative Toxicology and Ecotoxicology, , Viale Regina Elena 299, 00161 Rome, Italy
    Prog Nucleic Acid Res Mol Biol 68:3-27. 2001
    ..These findings raise the interesting possibility that different BER pathways might be acting as a function of the cell cycle stage...
  37. ncbi Yeast base excision repair: interconnections and networks
    P W Doetsch
    Departments of Biochemistry and Radiation Oncology, Emory University, Atlanta, Georgia 30322, USA
    Prog Nucleic Acid Res Mol Biol 68:29-39. 2001
    ..cerevisiae. Our results also suggest a physiological role for the AP lyase activity of certain BER N-glycosylases in vivo...
  38. ncbi Mechanism of action of Escherichia coli formamidopyrimidine N-glycosylase: role of K155 in substrate binding and product release
    L Rabow
    Division of Cancer Biology, Department of Radiation Oncology, Emory University School of Medicine, Atlanta, Georgia 30335, USA
    Prog Nucleic Acid Res Mol Biol 68:223-34. 2001
    ..This is consistent with the idea that K155A dissociates prematurely from the covalent complex, leading to a higher turnover number observed for K155A for DNA substrate containing an AP site...
  39. ncbi Multiple DNA glycosylases for repair of 8-oxoguanine and their potential in vivo functions
    T K Hazra
    Sealy Center for Molecular Science, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Prog Nucleic Acid Res Mol Biol 68:193-205. 2001
    ..The physiological significance of multiple OGGs and their in vivo reaction mechanisms remain to be elucidated by further studies...
  40. ncbi Molecular mechanism of PCNA-dependent base excision repair
    Y Matsumoto
    Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Prog Nucleic Acid Res Mol Biol 68:129-38. 2001
    ..Therefore, UNG2 and MYH may serve for replication-coupled base excision repair through the direct interaction with PCNA in the replication machinery...
  41. ncbi Mammalian Ogg1/Mmh gene plays a major role in repair of the 8-hydroxyguanine lesion in DNA
    S Nishimura
    Banyu Tsukuba Research Institute in collaboration with Merck Research Laboratories, Okubo 3, Tsukuba, Ibaraki 300-2611, Japan
    Prog Nucleic Acid Res Mol Biol 68:107-23. 2001
    ..These results indicate that exposure of DNA to internal oxidative species constantly produces the mutagenic DNA adduct 8-OH-G in mice, and that Mmh plays an essential role in the repair of this type of oxidative DNA damage...
  42. ncbi Structure and activity of a thermostable thymine-DNA glycosylase: evidence for base twisting to remove mismatched normal DNA bases
    Clifford D Mol
    Department of Molecular Biology MB4, Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Mol Biol 315:373-84. 2002
    ....
  43. pmc DNA polymerase beta is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts
    S L Allinson
    MRC Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK
    EMBO J 20:6919-26. 2001
    ..We find that, when repair synthesis proceeds through a Pol beta-dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol beta is essential for repair of both lesions...
  44. ncbi Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV
    J S Sung
    Departments of Environmental and Molecular Toxicology and Biochemistry and Biophysics and the Environmental Health Science Center, Oregon State University, Corvallis 97731, USA
    Biochemistry 39:10224-35. 2000
    ..G mispair. Analysis of reaction products revealed a time dependent appearance of repaired form I DNA; addition of purified Dug to the cell extract stimulated the rate of repair...
  45. ncbi Regulatory roles of p21 and apurinic/apyrimidinic endonuclease 1 in base excision repair
    S Tom
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 276:48781-9. 2001
    ..This model implies that BER can be regulated differentially, based upon the assembly of relevant proteins around APE1 in the presence or absence of PCNA...
  46. pmc The stalling of transcription at abasic sites is highly mutagenic
    Sung Lim Yu
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1061, USA
    Mol Cell Biol 23:382-8. 2003
    ..From the various observations presented here, we infer that the stalling of transcription at AP sites is highly mutagenic...
  47. ncbi High accumulation of oxidative DNA damage, 8-hydroxyguanine, in Mmh/Ogg1 deficient mice by chronic oxidative stress
    Tsuyoshi Arai
    Banyu Tsukuba Research Institute in collaboration with Merck Research Laboratories, 3 Okubo, Ibaraki 300 2611, Japan
    Carcinogenesis 23:2005-10. 2002
    ..These results indicate that 8-OH-G, produced by chronic exposure to exogenous oxidative stress agents, is not repaired to any significant extent within the overall genome of Ogg1(-/-) mice kidney...
  48. ncbi Cross-linking of 2-deoxyribonolactone and its beta-elimination product by base excision repair enzymes
    Kelly M Kroeger
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 42:2449-55. 2003
    ....
  49. pmc Mitochondrial targeting of human DNA glycosylases for repair of oxidative DNA damage
    M Takao
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai 980 77, Japan
    Nucleic Acids Res 26:2917-22. 1998
    ..These results suggest that the previously observed removal of oxidative base lesions in mitochondrial DNA is initiated by the above DNA glycosylases...
  50. ncbi The base substitution fidelity of DNA polymerase beta-dependent single nucleotide base excision repair
    Toshiro Matsuda
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:25947-51. 2003
    ..Thus other proteins in the BER reaction may enhance the base substitution fidelity of DNA polymerase beta during single nucleotide BER...
  51. pmc Endonuclease V (nfi) mutant of Escherichia coli K-12
    G Guo
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602, USA
    J Bacteriol 180:46-51. 1998
    ....
  52. pmc Suppressed catalytic activity of base excision repair enzymes on rotationally positioned uracil in nucleosomes
    Brian C Beard
    Department of Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4660, USA
    Proc Natl Acad Sci U S A 100:7465-70. 2003
    ..e., pol beta) may require nucleosome remodeling for efficient DNA BER in at least some regions of chromatin in eukaryotic cells...
  53. ncbi Base excision repair activities required for yeast to attain a full chronological life span
    Morag J Maclean
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC 1E 6BT, UK
    Aging Cell 2:93-104. 2003
    ..They also reveal an appreciable overlap in the G0 maintenance functions of the different BER DNA glycosylases and AP endonucleases...
  54. ncbi Bistranded oxidized purine damage clusters: induced in DNA by long-wavelength ultraviolet (290-400 nm) radiation?
    Joon Myong Song
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
    Biochemistry 41:8683-8. 2002
    ....
  55. ncbi cDNA and deduced amino acid sequence of a mouse DNA repair enzyme (APEX nuclease) with significant homology to Escherichia coli exonuclease III
    S Seki
    Department of Molecular Biology, Okayama University Medical School, Japan
    J Biol Chem 266:20797-802. 1991
    ..The deduced amino acid sequence of mouse APEX nuclease exhibits a significant homology to those of exonuclease III of E. coli and ExoA protein of Streptococcus pneumoniae and an intensive homology with that of bovine AP endonuclease 1...
  56. ncbi Oxidoreductive modification of two cysteine residues in paired domain by Ref-1 regulates DNA-binding activity of Pax-8
    Xia Cao
    Division of Molecular and Cellular Adaptation, Department of Endocrinology and Metabolism, Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Japan
    Biochem Biophys Res Commun 297:288-93. 2002
    ..These results demonstrated that oxidoreductive modification of Cys-45 and Cys-57 via Ref-1 plays a role in redox regulation of Pax-8 in living cells...
  57. pmc Characterization of a thermostable DNA glycosylase specific for U/G and T/G mismatches from the hyperthermophilic archaeon Pyrobaculum aerophilum
    H Yang
    Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Bacteriol 182:1272-9. 2000
    ..The unique conserved amino acid motifs of MIG proteins are proposed to distinguish MIG proteins from the closely related Nth/MutY DNA glycosylases...
  58. ncbi Maintenance of mitochondrial DNA integrity: repair and degradation
    Dongchon Kang
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Curr Genet 41:311-22. 2002
    ..In addition to DNA repair, the degradation of damaged mtDNA may be tolerated because there are multiple copies of mtDNA molecules in a cell...
  59. ncbi DNA damage repair in bladder urothelium after the Chernobyl accident in Ukraine
    Alina Romanenko
    Department of Pathology, Institute of Urology and Nephrology, Academy of Medical Sciences of Ukraine, Kiev, Ukraine
    J Urol 168:973-7. 2002
    ....
  60. pmc Stimulation of 3'-->5' exonuclease and 3'-phosphodiesterase activities of yeast apn2 by proliferating cell nuclear antigen
    Ildiko Unk
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555 1061, USA
    Mol Cell Biol 22:6480-6. 2002
    ..In the absence of DNA, Apn2 binds PCNA through its IDCL domain, whereas in the presence of DNA, when PCNA has been loaded onto the template-primer junction by replication factor C, the C-terminal domain of PCNA mediates the binding...
  61. pmc Repair of DNA lesions induced by hydrogen peroxide in the presence of iron chelators in Escherichia coli: participation of endonuclease IV and Fpg
    R S Galhardo
    Laboratório de Radiobiologia Molecular, Programa de Biologia Molecular, Instituto de Biofisica Carlos Chagas Filho, Centro de Ciências da Saúde Bloco G, Universidade Federal do Rio de Janeiro, CEP 21949 900 Rio de Janeiro, RJ, Brazil
    J Bacteriol 182:1964-8. 2000
    ....
  62. ncbi Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis
    D J Hosfield
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 98:397-408. 1999
    ....
  63. ncbi Truncation of amino-terminal tail stimulates activity of human endonuclease III (hNTH1)
    Xiang Liu
    Division of Carcinogenesis and Molecular Epidemiology, American Health Foundation Cancer Center, 1 Dana Road, Valhalla, NY 10595, USA
    J Mol Biol 321:265-76. 2002
    ..The inhibitory role of the N-terminal tail in catalytic turnover explains the low activity of hNTH1 compared to that of its E.coli homologue...
  64. pmc Quantitative comparison of catalytic mechanisms and overall reactions in convergently evolved enzymes: implications for classification of enzyme function
    Daniel E Almonacid
    Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, United States of America
    PLoS Comput Biol 6:e1000700. 2010
    ..The results also indicate that mechanistic convergence of reaction steps is widespread, suggesting that quantitative measurement of mechanistic similarity can inform approaches for functional annotation...
  65. ncbi Mutational analysis of O-acetylserine (thiol) lyase conducted in yeast two-hybrid system
    Frantz Liszewska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul Pawinskiego 5a, Warsaw, Poland
    Biochim Biophys Acta 1774:450-5. 2007
    ..Effects of mutagenesis were verified in a yeast two-hybrid model that allowed monitoring both, protein-protein interactions and the enzymatic activity of OASTL...
  66. ncbi Enrichment of tomato flavor by diversion of the early plastidial terpenoid pathway
    Rachel Davidovich-Rikanati
    Department of Vegetable Crops, Newe Ya ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay, 30095 Israel
    Nat Biotechnol 25:899-901. 2007
    ..Monoterpene accumulation was at the expense of reduced lycopene accumulation. Similar approaches may be applicable for carotenoid-accumulating fruits and flowers of other species...
  67. ncbi Cloning, expression, and characterization of a novel methylglyoxal synthase from Thermus sp. strain GH5
    Mohammad Pazhang
    Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
    Appl Biochem Biotechnol 162:1519-28. 2010
    ..Gel filtration experiments showed that TMGS is a hexameric enzyme, and its quaternary structure did not change in the presence of phosphate...
  68. ncbi APE/Ref-1 is controlled by both redox and cAMP-dependent mechanisms in rat thyroid cells
    G Tell
    Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine M A T I Center, Udine, Italy
    Horm Metab Res 34:303-10. 2002
    ..In contrast, the cytoplasm-to-nucleus translocation is dependent on redox-mediated mechanisms. Based upon the data shown in this study and in others, a bimodal control of APE/Ref-1 by TSH can be delineated...
  69. ncbi Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria
    Daniel Agren
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 283:31567-74. 2008
    ..The existence of an alternative biosynthetic pathway to cysteine in this pathogen has implications for the design strategy aimed at inhibition of this metabolic route...
  70. ncbi Apurinic/apyrimidinic endonuclease activity is elevated in human adult gliomas
    M S Bobola
    Department of Neurological Surgery, University of Washington, 1959 N E Pacific Street, Seattle, WA 98195, USA
    Clin Cancer Res 7:3510-8. 2001
    ....
  71. pmc Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate
    Rachel A Okrent
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    J Biol Chem 284:9742-54. 2009
    ..This suggests a general mechanism for the rapid, reversible regulation of GH3 activity and small molecule cross-talk. For PBS3, this may allow for coordination of flux through diverse chorismate-derived pathways...
  72. ncbi Brain expression of apurinic/apyrimidinic endonuclease (APE/Ref-1) multifunctional DNA repair enzyme gene in the mouse with special reference to the suprachiasmatic nucleus
    Hidehito Kimura
    Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Neurosci Res 46:443-52. 2003
    ..In the circadian center SCN, levels of APE/ref-1 mRNA transcripts were constantly high, and were not influenced by either circadian rhythms or by exposure to light...
  73. pmc Embryonic extracts derived from the nematode Caenorhabditis elegans remove uracil from DNA by the sequential action of uracil-DNA glycosylase and AP (apurinic/apyrimidinic) endonuclease
    Andrea Shatilla
    University of Montreal, Maisonneuve Rosemont Hospital, Guy Bernier Research Centre, 5415 de l Assomption, Montreal, Quebec, Canada H1T 2M4
    Biochem J 365:547-53. 2002
    ..It would appear that these activities correspond to the first two steps of a putative base-excision-repair pathway in C. elegans...
  74. ncbi Characterization of the hom-thrC-thrB cluster in aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331
    Mónica Fernández
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, Avda del Real, No 1, 24006 Leon, Spain
    Microbiology 148:1413-20. 2002
    ..NRRL 5331 and suggested that the branching point of the aspartic acid-derived biosynthetic route of this metabolite should lie earlier on the threonine biosynthetic route...
  75. ncbi Cystathionine gamma-synthase and threonine synthase operate in concert to regulate carbon flow towards methionine in plants
    Rachel Amir
    Plant Science Laboratory, MIGAL Galilee Technological Center, PO Box 90000, Rosh Pina 12100, Israel
    Trends Plant Sci 7:153-6. 2002
    ....
  76. ncbi Methylglyoxal synthetase, enol-pyruvaldehyde, glutathione and the glyoxalase system
    Irwin A Rose
    Department of Physiology and Biophysics, University of California, Irvine, Irvine, California 92717 1700, USA
    J Am Chem Soc 124:13047-52. 2002
    ....
  77. ncbi The exonuclease activity of human apurinic/apyrimidinic endonuclease (APE1). Biochemical properties and inhibition by the natural dinucleotide Gp4G
    Kai Ming Chou
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:18289-96. 2003
    ..APE1 may exist in two different conformations, and each conformation has a preference for a substrate. The different conformations can be affected by MgCl(2) or salt concentrations...
  78. ncbi Nutritional modulation of DNA repair in a human intervention study
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, POB 1046 Blindern, N 0316 Oslo, Norway
    Carcinogenesis 24:511-5. 2003
    ..Kiwifruit provides a dual protection against oxidative DNA damage, enhancing antioxidant levels and stimulating DNA repair. It is probable that together these effects would decrease the risk of mutagenic changes leading to cancer...
  79. ncbi Redox factor-1 contributes to the regulation of progression from G0/G1 to S by PDGF in vascular smooth muscle cells
    Tongrong He
    Free Radical and Radiation Biology Program, Dept of Radiation Oncology, The University of Iowa, Iowa City, IA 52242 1181, USA
    Am J Physiol Heart Circ Physiol 285:H804-12. 2003
    ..These results suggest that Ref-1 contributes to the regulation of PDGF-BB-stimulated cell cycle progression from G0/G1 to S in SMCs, with one of the possible steps being redox-regulation of AP-1 by Ref-1 protein...
  80. ncbi Modulation of glutathione and thioredoxin systems by calorie restriction during the aging process
    Chong Gun Cho
    Department of Molecular Pathology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229 3900, USA
    Exp Gerontol 38:539-48. 2003
    ..Our conclusion is that a redox imbalance occurs during aging and that redox changes are minimized through the anti-oxidative action of CR...
  81. ncbi O-acetylserine (thiol) lyase activity in Phragmites and Typha plants under cadmium and NaCl stress conditions and the involvement of ABA in the stress response
    Erika Fediuc
    Biostress Research Laboratory, J Blaustein Institute for Desert Researches, Ben Gurion University of the Negev, Sede Boqer Campus, 84990 Israel
    J Plant Physiol 162:865-72. 2005
    ..The increase of ABA content indicates the involvement of this phytohormone in early stress responses, while the stimulation of OASTL following the ABA application suggests that ABA has a role in an OASTL activation pathway...
  82. ncbi Effect of glucose on assimilatory sulphate reduction in Arabidopsis thaliana roots
    Holger Hesse
    Max Planck Institute for Molecular Plant Physiology, Department of Molecular Physiology, Am Muehlenberg 1, D 14476 Golm, Germany
    J Exp Bot 54:1701-9. 2003
    ..Furthermore, signals originating from nitrogen and carbon metabolism regulate APR synergistically...
  83. ncbi The molecular chaperone Hsp90 mediates heme activation of the yeast transcriptional activator Hap1
    Hee Chul Lee
    Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
    J Biol Chem 277:7430-7. 2002
    ..These experiments together suggest that Hsp90 promotes Hap1 activation by inducing or maintaining Hap1 in a transcriptionally active conformation...
  84. ncbi Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae
    J Hansen
    Carlsberg Research Laboratory, Copenhagen, Valby, Denmark
    Mol Gen Genet 263:535-42. 2000
    ..While some repression of MET genes could be accomplished by homocysteine in a strain that cannot form SAM from methionine, a low intracellular level of SAM seems to be necessary for full cysteine-mediated repression to take place...
  85. ncbi Roles of O-acetyl-L-homoserine sulfhydrylases in micro-organisms
    S Yamagata
    Department of Biology, Faculty of General Education, Gifu University, Japan
    Biochimie 71:1125-43. 1989
    ..The protein can also catalyze the de novo synthesis of L-cysteine and O-alkyl-L-homoserine in some microorganisms. The enzyme possibly recycles the methylthio group of methionine...
  86. pmc Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene
    D Thomas
    Laboratoire d Enzymologie du Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Mol Cell Biol 9:3292-8. 1989
    ..Moreover, we demonstrate that in addition to this transcriptional regulation, a posttranscriptional regulation, probably targeted at the 5' region of mRNA, is involved in MET25 expression...
  87. ncbi 5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro
    K Bebenek
    Laboratory of Molecular Genetics and, Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Science 291:2156-9. 2001
    ..These data and three distinct catalytic properties of pol iota implicate it in specialized forms of base excision repair (BER)...
  88. ncbi Effects of the amplification of the genes coding for the L-threonine biosynthetic enzymes on the L-threonine production from methanol by a gram-negative obligate methylotroph, Methylobacillus glycogenes
    H Motoyama
    Tokyo Research Laboratory, Kyowa Hakko Kogyo Co Ltd, Japan
    Appl Microbiol Biotechnol 42:67-72. 1994
    ..The effects of gene amplification were confirmed by the cultivation in 5-1 jar fermentors. The best producer, an A513 transconjugant containing the plasmid carrying the hom-thrC genes, produced 16.3 g/l L-threonine for 72 h...
  89. ncbi Evolutionary comparisons of three enzymes of the threonine biosynthetic pathway among several microbial species
    B Cami
    Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, Marseille, France
    Biochimie 75:487-95. 1993
    ....
  90. ncbi Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli
    M C Wahl
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Planegg Martinsried, Germany
    FEBS Lett 414:492-6. 1997
    ..The crystallographic results corroborate and extend previous solution studies regarding the quaternary organization of the enzyme...
  91. pmc Cloning and nucleotide sequences of the homoserine dehydrogenase genes (hom) and the threonine synthase genes (thrC) of the gram-negative obligate methylotroph Methylobacillus glycogenes
    H Motoyama
    Tokyo Research Laboratories, Kyowa Hakko Kogyo Co Ltd, Japan
    Appl Environ Microbiol 60:111-9. 1994
    ..Northern (RNA) blot analysis suggests that the hom and thrC genes are organized in an operon. Significant homology between the predicted amino acid sequences of the hom and thrC genes and those from other microorganisms was found...
  92. pmc Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri
    J Belfaiza
    , , El Jadida, Morocco
    J Bacteriol 180:250-5. 1998
    ..Our results indicate, therefore, that the L. meyeri MetY enzyme is able to perform direct sulfhydrylation for methionine biosynthesis by using O-acetylhomoserine as a substrate...
  93. pmc Transcriptional repression at a distance through exclusion of activator binding in vivo
    M Shimizu
    School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Proc Natl Acad Sci U S A 94:790-5. 1997
    ..Thus Rme1p may alter chromatin to prevent binding of transcriptional activators to distant DNA sequences...
  94. ncbi Characterization of Bacillus subtilis ExoA protein: a multifunctional DNA-repair enzyme similar to the Escherichia coli exonuclease III
    T Shida
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan
    Biosci Biotechnol Biochem 63:1528-34. 1999
    ..Thus, it has been confirmed that ExoA is a multifunctional DNA-repair enzyme in B. subtilis that is very similar to E. coli exonuclease III except that ExoA has lower 3'-5' exonuclease activity than that of E. coli exonuclease III...
  95. ncbi Regulation of sulphate assimilation in Saccharomyces cerevisiae
    B Ono
    Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan
    Yeast 12:1153-62. 1996
    ..e. cysteine inhibits SATase to lower the cellular concentration of OAS which is required for induction of the sulphate assimilation enzymes including OAS/OAH SHLase. Subsequently, we obtained data supporting this speculation...
  96. pmc The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis
    R A Bennett
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 19:1800-9. 1999
    ..Thus, in apn1 cells where the major AP endonuclease activity is missing, ETH1 offers an alternate capacity for repair of spontaneous or induced damage to DNA that is normally repaired by Apn1 protein...
  97. ncbi Genetic interaction between HAP1/REF-1 and p53
    L B Meira
    Nat Genet 17:145. 1997
  98. ncbi Sequence analysis identifies TTRAP, a protein that associates with CD40 and TNF receptor-associated factors, as a member of a superfamily of divalent cation-dependent phosphodiesterases
    F Rodrigues-Lima
    Faculté de Médecine Pitié Salpêtrière, CNRS UMR 7000, Paris, 75013, France
    Biochem Biophys Res Commun 285:1274-9. 2001
    ..This potential novel function of TTRAP raises the intriguing possibility for a role of APE1-like DNA-repair endonucleases in TNF receptor family-mediated signaling and functions...
  99. pmc Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites
    R E Johnson
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    Genes Dev 12:3137-43. 1998
    ..Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites...
  100. ncbi Structure and regulation of cysD, the homocysteine synthase gene of Aspergillus nidulans
    M Sienko
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawi nskiego 5a, 02 106 Warsaw, Poland
    Curr Genet 33:136-44. 1998
    ..nidulans strains, revealed a marked DNA-sequence polymorphism manifested mostly by silent point mutations. There was, however, much less polymorphism in the protein sequence...
  101. ncbi The apurinic-apyrimidinic endonuclease IV family of DNA repair enzymes
    D Ramotar
    Hopital Maisonneuve Rosemont, Centre de Recherche, Universite de Montreal, QC, Canada
    Biochem Cell Biol 75:327-36. 1997
    ..These AP endonucleases have evolved not only to repair AP sites but also to correct distinct DNA lesions...

Research Grants22

  1. OXIDATIVE STRESS--CELLULAR RESPONSES AND GENE CONTROL
    Bruce Demple; Fiscal Year: 2004
    ..The knowledge gained from these studies will illuminate approaches to dissecting the mechanisms and roles of redox and NO signal transduction in all organisms. ..
  2. Society for Research on Biological Rhythms Meeting
    Carla Green; Fiscal Year: 2006
    ..A goal of the 2006 meeting is to bring in a number of excellent scientists from outside fields to increase the inter-disciplinary aspects of clock studies. [unreadable] [unreadable] [unreadable]..
  3. Imbalancing DNA BER to enhance Ovarian Tumor Sensitivity
    Mark Kelley; Fiscal Year: 2008
    ..abstract_text> ..
  4. Therapeutic/Mechanistic Role of Ape1 in Germ Cell Tumors
    Mark Kelley; Fiscal Year: 2007
    ..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
  5. OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLS
    Mark Kelley; Fiscal Year: 2003
    ..abstract_text> ..
  6. REGULATION OF ALVEOLAR MACROPHAGE FUNCTION
    DAWN FLAHERTY; Fiscal Year: 2005
    ..The studies are important because the shift in phenotype of macrophages in the lung may be critical to the pathogenesis of a number of chronic lung disorders. ..
  7. PRECANCEROUS EVENTS IN CLINICALLY NORMAL SKIN
    Douglas Brash; Fiscal Year: 2001
    ....
  8. Ape1, oxidative stress and glioma alkylator resistance
    JOHN SILBER; Fiscal Year: 2008
    ..Evidence for a contribution of Ap endo to resistance would identify a new target for anti-resistance strategies directed against Ape1/Ref-1 (the major human Ap endo), either alone or together with MGMT. ..
  9. Ap endo as a predictor of response to glioma therapy
    JOHN SILBER; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  10. How Substrates Activate Fumarase and Glyoxalase Evolved
    Irwin Rose; Fiscal Year: 2005
    ..Since methylglyoxal is toxic to cells and must be removed by an active glyoxalse system, inhibition of a MSH based system could result in a treatment for many mycobacterial-based diseases such as tuberculosis. ..
  11. COMBATING ALKYLATING AGENT RESISTANCE IN HUMAN GLIOMAS
    JOHN SILBER; Fiscal Year: 2002
    ..In an effort to define the role of 3-MAG in clinical drug resistance, enzyme levels will be correlated with response to alkylator adjuvant therapy and clinical course. ..
  12. Pineal Cell Biology Gordon Conference
    Carla Green; Fiscal Year: 2002
    ..The previous meetings have been quite successful, and attendance of this conference has steadily increased such that the last meeting was well over-subscribed. ..
  13. Repair of Oxidatively Damaged Guanines
    A Lien Lu Chang; Fiscal Year: 2008
    ..We anticipate that these studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. ..
  14. DNA Damage Clusters: Repair in Mammalian Cells
    Betsy Sutherland; Fiscal Year: 2004
    ..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..
  15. A Bioinformatic Approach to Inferring Protein Contacts
    MARCELLA MCCLURE; Fiscal Year: 2005
    ..Several experimental laboratories have agreed to test the residue contact predictions resulting from these studies for Ebola, VSV and measles. ..
  16. SUNLIGHT-RELATED STEPS IN HUMAN SKIN CANCER
    Douglas Brash; Fiscal Year: 2007
    ..Together, these studies may reveal both DNA damage and repair variations across the genome and mechanisms underlying them. Project Description Page 6 ..
  17. IN VIVO STUDIES OF VERTEBRATE CIRCADIAN CLOCK GENES
    Ignacio Provencio; Fiscal Year: 2008
    ..Because these clocks are conserved, information gained from these studies will provide insight into vertebrate clocks in general, including those in humans. [unreadable] [unreadable]..
  18. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2008
    ..Results from these experiments will support future microbiological studies of the role of this system in chlamydial infection and survival in the host cell. [unreadable] [unreadable] [unreadable]..
  19. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
    ..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
  20. Visualizing Clonal Expansion in Living Mice
    Douglas Brash; Fiscal Year: 2005
    ..abstract_text> ..
  21. Retrovirus Restriction by Trima5alpha
    Paul Bieniasz; Fiscal Year: 2009
    ....