acetyl coa carboxylase

Summary

Summary: A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.

Top Publications

  1. ncbi Contraction-induced changes in acetyl-CoA carboxylase and 5'-AMP-activated kinase in skeletal muscle
    D Vavvas
    Department of Physiology and Diabetes and Metabolism Unit, Evans Department of Medicine, Boston University Medical Center, Boston, Massachusetts 02118, USA
    J Biol Chem 272:13255-61. 1997
  2. ncbi Thiol-disulfide exchange between nuclear-encoded and chloroplast-encoded subunits of pea acetyl-CoA carboxylase
    A Kozaki
    Laboratory of Plant Molecular Biology, Graduate School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
    J Biol Chem 276:39919-25. 2001
  3. ncbi RNA interference-mediated silencing of the acetyl-CoA-carboxylase-alpha gene induces growth inhibition and apoptosis of prostate cancer cells
    Koen Brusselmans
    Laboratory for Experimental Medicine and Endocrinology, University of Leuven, Leuven, Belgium
    Cancer Res 65:6719-25. 2005
  4. ncbi Soy protein suppresses gene expression of acetyl-coA carboxylase alpha from promoter PI in rat liver
    Hisa Aoki
    Course of the Science of Bioresources, The United Graduate School of Agricultural Sciences, Iwate University, Iwate, Japan
    Biosci Biotechnol Biochem 70:843-9. 2006
  5. ncbi Acetyl-CoA carboxylase alpha is essential to breast cancer cell survival
    Veronique Chajes
    Centre National de la Recherche Scientifique FRE 2939, Institut Gustave Roussy, Villejuif, France
    Cancer Res 66:5287-94. 2006
  6. ncbi Plant biotin-containing carboxylases
    Basil J Nikolau
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Arch Biochem Biophys 414:211-22. 2003
  7. ncbi Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase
    Simon A Hawley
    Division of Molecular Physiology University of Dundee, Dundee, Scotland, United Kingdom
    Cell Metab 2:9-19. 2005
  8. pmc Acc homoeoloci and the evolution of wheat genomes
    D Chalupska
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 105:9691-6. 2008
  9. pmc Acetyl-CoA carboxylase 2 mutant mice are protected against obesity and diabetes induced by high-fat/high-carbohydrate diets
    Lutfi Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:10207-12. 2003
  10. ncbi Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    FASEB J 19:1146-8. 2005

Detail Information

Publications238 found, 100 shown here

  1. ncbi Contraction-induced changes in acetyl-CoA carboxylase and 5'-AMP-activated kinase in skeletal muscle
    D Vavvas
    Department of Physiology and Diabetes and Metabolism Unit, Evans Department of Medicine, Boston University Medical Center, Boston, Massachusetts 02118, USA
    J Biol Chem 272:13255-61. 1997
    ..These alterations in ACC and AMPK activity, by diminishing the concentration of malonyl-CoA, could be responsible for the increase in fatty acid oxidation observed in skeletal muscle during exercise...
  2. ncbi Thiol-disulfide exchange between nuclear-encoded and chloroplast-encoded subunits of pea acetyl-CoA carboxylase
    A Kozaki
    Laboratory of Plant Molecular Biology, Graduate School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
    J Biol Chem 276:39919-25. 2001
    ..A covalent bond between a nuclear-encoded polypeptide and a chloroplast-encoded polypeptide probably regulates the enzyme activity in response to light...
  3. ncbi RNA interference-mediated silencing of the acetyl-CoA-carboxylase-alpha gene induces growth inhibition and apoptosis of prostate cancer cells
    Koen Brusselmans
    Laboratory for Experimental Medicine and Endocrinology, University of Leuven, Leuven, Belgium
    Cancer Res 65:6719-25. 2005
    ....
  4. ncbi Soy protein suppresses gene expression of acetyl-coA carboxylase alpha from promoter PI in rat liver
    Hisa Aoki
    Course of the Science of Bioresources, The United Graduate School of Agricultural Sciences, Iwate University, Iwate, Japan
    Biosci Biotechnol Biochem 70:843-9. 2006
    ..These data suggest that SPI feeding decreased the hepatic contents of ACC alpha mRNA mainly by regulating PI promoter via a nuclear factor(s) other than SREBP-1...
  5. ncbi Acetyl-CoA carboxylase alpha is essential to breast cancer cell survival
    Veronique Chajes
    Centre National de la Recherche Scientifique FRE 2939, Institut Gustave Roussy, Villejuif, France
    Cancer Res 66:5287-94. 2006
    ..These data confirm the importance of lipogenesis in cancer cell survival and indicate that this pathway represents a key target for antineoplastic therapy that, however, might require specific dietary recommendation for full efficacy...
  6. ncbi Plant biotin-containing carboxylases
    Basil J Nikolau
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Arch Biochem Biophys 414:211-22. 2003
    ..This minireview summarizes the recent developments in our understanding of the structure, regulation, and metabolic functions of these proteins in plants...
  7. ncbi Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase
    Simon A Hawley
    Division of Molecular Physiology University of Dundee, Dundee, Scotland, United Kingdom
    Cell Metab 2:9-19. 2005
    ..Our results suggest a potential Ca(2+)-dependent neuroprotective pathway involving phosphorylation and activation of AMPK by CaMKKbeta...
  8. pmc Acc homoeoloci and the evolution of wheat genomes
    D Chalupska
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 105:9691-6. 2008
    ..5 Mya. Such variation might result in a significant overestimate of the time since tetraploid wheat formation, which occurred no more than 0.5 Mya...
  9. pmc Acetyl-CoA carboxylase 2 mutant mice are protected against obesity and diabetes induced by high-fat/high-carbohydrate diets
    Lutfi Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:10207-12. 2003
    ..These results suggest that ACC2 plays an essential role in controlling fatty acid oxidation and is a potential target in therapy against obesity and related diseases...
  10. ncbi Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    FASEB J 19:1146-8. 2005
    ....
  11. ncbi Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2
    L Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 291:2613-6. 2001
    ..The mutant mice accumulated 50% less fat in their adipose tissue than did wild-type mice. These results raise the possibility that pharmacological manipulation of ACC2 may lead to loss of body fat in the context of normal caloric intake...
  12. ncbi Multi-subunit acetyl-CoA carboxylases
    John E Cronan
    Department of Microbiology, B103 Chemical and Life Sciences Laboratory, University of Illinois, 601 S Goodwin Avenue, Urbana, IL 61801, USA
    Prog Lipid Res 41:407-35. 2002
    ..Mammals, fungi, and plant cytosols contain the second type of ACC, a single large multifunctional polypeptide. This review will focus on the structures, regulation, and enzymatic mechanisms of the bacterial and plant MS-ACCs...
  13. ncbi Structure and regulation of acetyl-CoA carboxylase genes of metazoa
    Michael C Barber
    Hannah Research Institute, Ayr, KA6 5HL, Scotland, United Kingdom
    Biochim Biophys Acta 1733:1-28. 2005
    ..This review focuses on the structure of ACC genes and the putative individual roles of their gene products in fatty acid metabolism, taking an evolutionary viewpoint provided by data in genome databases...
  14. pmc Identification of a second human acetyl-CoA carboxylase gene
    J Widmer
    Department of Medicine, Dartmouth Medical School, Hanover NH 03755, USA
    Biochem J 316:915-22. 1996
    ..The newly discovered human gene and its rat homologue appear to be under different regulatory control to the HACC265 gene, as judged by tissue-specific expression in vivo and by independent modulation in cultured cells in vitro...
  15. ncbi Phylogenetic analysis of the acetyl-CoA carboxylase and 3-phosphoglycerate kinase loci in wheat and other grasses
    Shaoxing Huang
    Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637, USA
    Plant Mol Biol 48:805-20. 2002
    ..The ACCase and PGK genes provide a well-understood two-locus system to study grass phylogeny, evolution and systematics...
  16. ncbi Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction
    Song Xiang
    Department of Biological Sciences, 212 Amsterdam Avenue, Columbia University, New York, New York 10027, USA
    Nat Struct Mol Biol 15:295-302. 2008
    ..There are dramatic differences in domain positions in the monomer and the organization of the tetramer between these enzymes and the PC from Rhizobium etli...
  17. ncbi Isozyme-nonselective N-substituted bipiperidylcarboxamide acetyl-CoA carboxylase inhibitors reduce tissue malonyl-CoA concentrations, inhibit fatty acid synthesis, and increase fatty acid oxidation in cultured cells and in experimental animals
    H James Harwood
    Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development, Groton Laboratories, Pfizer Inc, Groton, Connecticut 06340, USA
    J Biol Chem 278:37099-111. 2003
    ..Taken together, These observations indicate that isozyme-nonselective ACC inhibition has the potential to favorably affect risk factors associated with the metabolic syndrome...
  18. pmc Mutant mice lacking acetyl-CoA carboxylase 1 are embryonically lethal
    Lutfi Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 102:12011-6. 2005
    ....
  19. ncbi Phylogenetic relationships between Hystrix and its closely related genera (Triticeae; Poaceae) based on nuclear Acc1, DMC1 and chloroplast trnL-F sequences
    Lina Sha
    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130 Sichuan, China
    Mol Phylogenet Evol 54:327-35. 2010
    ..Based on these results, we suggest that Hy. coreana, Hy. duthiei, Hy. duthiei ssp. longearistata, Hy. komarovii and Hy. californica should be included in the genus Leymus, and Hy. patula in the genus Elymus...
  20. pmc Expression, biotinylation and purification of a biotin-domain peptide from the biotin carboxy carrier protein of Escherichia coli acetyl-CoA carboxylase
    A Chapman-Smith
    Department of Biochemistry, University of Adelaide, Australia
    Biochem J 302:881-7. 1994
    ..The identify of the purified biotin-domain peptide was confirmed by N-terminal sequence analysis, amino acid analysis and m.s. The domain was readily produced and purified in sufficient quantities for n.m.r. structural analysis...
  21. ncbi Aging impairs insulin-stimulated glucose uptake in rat skeletal muscle via suppressing AMPKalpha
    Wan Qiang
    Department of Internal Medicine, Shandong Provincial Hospital Shandong University, Jinan 250021, China
    Exp Mol Med 39:535-43. 2007
    ..In conclusion, aging-related insulin resistance is associated with impaired AMPKalpha activity and could be ameliorated by AICAR, thus indicating a possible role of AMPK in aging-induced insulin resistance...
  22. ncbi Effect of AICAR treatment on glycogen metabolism in skeletal muscle
    William G Aschenbach
    Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:567-73. 2002
    ..Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake...
  23. ncbi A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product
    Yang Shen
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell 16:881-91. 2004
    ..Observations from native gel electrophoresis confirm this structural insight. The structural information provides a foundation for structure-based design of new inhibitors against these enzymes...
  24. ncbi Regulation of acetyl CoA carboxylase and carnitine palmitoyl transferase-1 in rat adipocytes
    Yan Zang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 13:1530-9. 2005
    b>Acetyl CoA carboxylase (ACC) is a key enzyme in energy balance. It controls the synthesis of malonyl-CoA, an allosteric inhibitor of carnitine palmitoyltransferase-1 (CPT-I). CPT-I is the gatekeeper of free fatty acid (FFA) oxidation...
  25. pmc Expression and characterization of recombinant fungal acetyl-CoA carboxylase and isolation of a soraphen-binding domain
    Stephanie C Weatherly
    Cropsolution, Inc, P O Box 14069, Research Triangle Park, NC 27560, USA
    Biochem J 380:105-10. 2004
    ..Together, these reagents will be useful for structural studies and assay development...
  26. ncbi Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery
    L Tong
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Cell Mol Life Sci 62:1784-803. 2005
    ..The current intense research on these enzymes could lead to the development of novel therapies against metabolic syndrome and other diseases...
  27. pmc Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation
    C Menendez
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Freiburg, Germany
    J Bacteriol 181:1088-98. 1999
    ..The results also provide further support for the occurrence of the 3-hydroxypropionate cycle in C. aurantiacus...
  28. ncbi Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation
    Michael Hügler
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Germany
    Eur J Biochem 270:736-44. 2003
    ..Due to its stability the archaeal carboxylase may prove an ideal subject for further structural studies...
  29. pmc Glucose and fat metabolism in adipose tissue of acetyl-CoA carboxylase 2 knockout mice
    Wonkeun Oh
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 102:1384-9. 2005
    ....
  30. pmc The subcellular localization of acetyl-CoA carboxylase 2
    L Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 97:1444-9. 2000
    ....
  31. ncbi The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme
    Patrick Bilder
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 45:1712-22. 2006
    ..0 and 3.0 A, respectively. Both structures reveal a small, independent zinc-binding domain that lacks a complement in the primary sequence or structure of the eukaryotic homologue...
  32. ncbi Selective activation of the fatty acid synthesis pathway in human prostate cancer
    J V Swinnen
    Laboratory for Experimental Medicine and Endocrinology, Gasthuisberg, Catholic University of Leuven, Belgium
    Int J Cancer 88:176-9. 2000
    ..These findings point to selective activation of the fatty acid synthesis pathway and suggest a shift in the balance of lipogenic gene expression in a subgroup of prostate cancers...
  33. ncbi Regulation of AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation by palmitate in skeletal muscle cells
    S Fediuc
    School of Kinesiology and Health Science, York University, Toronto, Canada
    J Lipid Res 47:412-20. 2006
    ..Here, we provide novel evidence that the AMPK/ACC pathway may operate as a mechanism to sense and respond to the lipid energy charge of skeletal muscle cells...
  34. ncbi The biotin enzyme family: conserved structural motifs and domain rearrangements
    Sarawut Jitrapakdee
    Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
    Curr Protein Pept Sci 4:217-29. 2003
    ..These structural homologies are so extensive as to be highly suggestive of evolutionary relationships between biotin carboxylases and these other enzymes...
  35. ncbi AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes
    Kurt Højlund
    Department of Human Physiology, Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 286:E239-44. 2004
    ..These data suggest that impaired insulin action on glycogen synthesis and lipid oxidation in skeletal muscle of obese type 2 diabetic subjects is unlikely to involve changes in AMPK expression and activity...
  36. ncbi Unique structural features and differential phosphorylation of the 280-kDa component (isozyme) of rat liver acetyl-CoA carboxylase
    R Winz
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada
    J Biol Chem 269:14438-45. 1994
    ....
  37. ncbi The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity
    A Chapman-Smith
    Dept of Biochemistry, University of Adelaide, Adelaide, Australia
    Trends Biochem Sci 24:359-63. 1999
    ..These, together with mutational analyses of biotinylated proteins, are beginning to elucidate the exceptional specificity of this protein modification...
  38. pmc Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis
    S J Li
    Department of Microbiology, University of Illinois, Urbana Champaign 61801
    J Bacteriol 175:332-40. 1993
    ..For example, the accA promoter was localized within the upstream polC gene (which encodes the DNA polymerase III catalytic subunit), suggesting that additional regulatory mechanisms exist...
  39. ncbi Identification by amino acid sequencing of three major regulatory phosphorylation sites on rat acetyl-CoA carboxylase
    M R Munday
    MRC Protein Phosphorylation Group, Biochemistry Department, University of Dundee, Tayside, Scotland
    Eur J Biochem 175:331-8. 1988
    ..They also open the way to experiments which could establish the physiological roles of these protein kinases in the control of fatty acid synthesis...
  40. ncbi The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase
    S J Li
    Department of Microbiology, University of Illinois, Urbana Champaign 61801
    J Biol Chem 267:16841-7. 1992
    ..Several conserved regions which may function as CoA-binding sites are noted...
  41. ncbi Human acetyl-CoA carboxylase 2. Molecular cloning, characterization, chromosomal mapping, and evidence for two isoforms
    L Abu-Elheiga
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 272:10669-77. 1997
    ..The regulation and physiological significance of the two ACC2 isoforms remain to be determined...
  42. pmc Plastid-localized acetyl-CoA carboxylase of bread wheat is encoded by a single gene on each of the three ancestral chromosome sets
    P Gornicki
    Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 94:14179-84. 1997
    ..The gene maps near the telomere on the short arm of chromosomes 2A, 2B, and 2D. Identification of three different cDNAs, two corresponding to genes Acc-1,1 and Acc-1,2, indicates that all three genes are transcriptionally active...
  43. ncbi Purification and characterization of intact and truncated forms of the Escherichia coli biotin carboxyl carrier subunit of acetyl-CoA carboxylase
    E Nenortas
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, 21228, USA
    J Biol Chem 271:7559-67. 1996
    ..Kinetic parameters estimated from these measurements indicate that the intact and truncated forms of the acceptor protein are functionally identical...
  44. ncbi Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing
    F K Athappilly
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Structure 3:1407-19. 1995
    ..In Escherichia coli, the three components reside in separate subunits: a biotinyl domain is the functional portion of one of these, biotin carboxy carrier protein (BCCP)...
  45. pmc Biochemical and molecular biological characterization of CAC2, the Arabidopsis thaliana gene coding for the biotin carboxylase subunit of the plastidic acetyl-coenzyme A carboxylase
    J Sun
    Department of Botany, Iowa State University, Ames 50011 1020, USA
    Plant Physiol 115:1371-83. 1997
    ..This hypothesis is consistent with the finding that the CAC2 and CAC1 gene promoters share a common set of sequence motifs that may be important in guiding the transcription of these genes...
  46. ncbi The biotinyl domain of Escherichia coli acetyl-CoA carboxylase. Evidence that the "thumb" structure id essential and that the domain functions as a dimer
    J E Cronan
    Departments of Microbiology and Biochemistry, University of Illinois, Urbana, Illinois 61801
    J Biol Chem 276:37355-64. 2001
    ..coli strain. This complementation was shown to be specific to BCCPs having native structure (excepting the biotinylated lysine) and is interpreted in terms of dimerization of the BCCP biotinyl domain during the ACC reaction...
  47. pmc Hepatic de novo lipogenesis is present in liver-specific ACC1-deficient mice
    Naomoto Harada
    Tsukuba Research Institute, Banyu Pharmaceutical Co, Ltd, 3 Okubo, Tsukuba, Ibaraki 300 2611, Japan
    Mol Cell Biol 27:1881-8. 2007
    ..Therefore, there does not appear to be strict compartmentalization of malonyl-CoA from either of the ACC isozymes in the liver...
  48. pmc Cloning of human acetyl-CoA carboxylase-beta and its unique features
    J Ha
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 93:11466-70. 1996
    ..A fragment of ACC-beta cDNA was expressed in Escherichia coli and antibodies against the peptide were generated to establish that the cDNA sequence that we cloned is that for ACC-beta...
  49. ncbi Three-dimensional structure of the biotin carboxylase subunit of acetyl-CoA carboxylase
    G L Waldrop
    Institute for Enzyme Research, Graduate School, University of Wisconsin, Madison 53705
    Biochemistry 33:10249-56. 1994
    ..Those amino acid residues believed to form part of the active site pocket include His 209-Glu 211, His 236-Glu 241, Glu 276, Ile 287-Glu 296, and Arg 338.2+ represents the first X-ray model of a biotin-dependent carboxylase...
  50. pmc Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase
    S Saitoh
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    J Cell Biol 134:949-61. 1996
    ..The cut6+ and lsd1+ genes are essential for viability and encode, respectively, acetyl CoA carboxylase and fatty acid synthetase, the key enzymes for fatty acid synthesis...
  51. ncbi Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase
    Hailong Zhang
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Science 299:2064-7. 2003
    ..Mutagenesis and kinetic studies reveal the functional roles of conserved residues here. The herbicides target the active site of CT, providing a lead for inhibitor development against human ACCs...
  52. ncbi Autotrophic carbon dioxide fixation in Acidianus brierleyi
    M Ishii
    Department of Biotechnology, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113, Japan
    Arch Microbiol 166:368-71. 1996
    ..brierleyi was growing autotrophically. We conclude that a modified 3-hydroxypropionate pathway operates in A. brierleyi...
  53. pmc DNA inhibits catalysis by the carboxyltransferase subunit of acetyl-CoA carboxylase: implications for active site communication
    Brian K Benson
    Division of Biochemistry and Molecular Biology, Louisana State University, Baton Rouge, Louisana 70803, USA
    Protein Sci 17:34-42. 2008
    ..An important mechanistic implication of these observations is that the dual active sites of ACC are functionally connected...
  54. ncbi Dissociation of AMPK activity and ACCbeta phosphorylation in human muscle during prolonged exercise
    Jørgen F P Wojtaszewski
    Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    Biochem Biophys Res Commun 298:309-16. 2002
    ....
  55. ncbi Increased Akt protein expression is associated with decreased ceramide content in skeletal muscle of troglitazone-treated mice
    Anna Planavila
    Pharmacology Unit, Department of Pharmacology and Therapeutic Chemistry, Faculty of Pharmacy, University of Barcelona, Spain
    Biochem Pharmacol 69:1195-204. 2005
    ..These findings implicate ceramide as an important intermediate in the regulation of Akt after troglitazone treatment...
  56. ncbi Expression of two Escherichia coli acetyl-CoA carboxylase subunits is autoregulated
    Ethan S James
    Departments of Microbiology and Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 279:2520-7. 2004
    ..These data support a model in which AccB acts as an autoregulator of accBC operon transcription...
  57. pmc ChREBP, but not LXRs, is required for the induction of glucose-regulated genes in mouse liver
    Pierre Damien Denechaud
    Institut Cochin, Departement d Endocrinologie, Métabolisme et Cancer, Universite Paris Descartes, CNRS, UMR 8104, Paris, France
    J Clin Invest 118:956-64. 2008
    ..Taken together, our results demonstrate that glucose is required for ChREBP functional activity and that LXRs are not necessary for the induction of glucose-regulated genes in liver...
  58. ncbi Tissue-specific regulation of acetyl-CoA carboxylase gene expression by dietary soya protein isolate in rats
    Chao Wu Xiao
    Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, 2203C Banting Research Centre, Ottawa, ON, Canada K1A 0L2
    Br J Nutr 95:1048-54. 2006
    ..The results indicate that the effect of SPI is tissue-specific and that alteration of ACC gene expression rather than phosphorylation status may play a major role in the regulation of ACC activities by soya proteins...
  59. ncbi Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase
    T Yamauchi
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Nat Med 8:1288-95. 2002
    ..Our data may provide a novel paradigm that an adipocyte-derived antidiabetic hormone, Ad, activates AMPK, thereby directly regulating glucose metabolism and insulin sensitivity in vitro and in vivo...
  60. ncbi Carboxylase genes of Sulfolobus metallicus
    N P Burton
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Arch Microbiol 172:349-53. 1999
    ..It is proposed that the three described genes could encode the key enzyme complex responsible for carbon dioxide fixation during autotrophic growth of the thermoacidophilic archaea...
  61. ncbi Critical phosphorylation sites for acetyl-CoA carboxylase activity
    J Ha
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907
    J Biol Chem 269:22162-8. 1994
    ....
  62. ncbi Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle
    Jorgen F P Wojtaszewski
    Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 2100 Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 284:E813-22. 2003
    ..It is concluded that muscle AMPK activity and ACCbeta Ser(221) phosphorylation at rest and during exercise are sensitive to the fuel status of the muscle. During exercise, this dependence may in part be mediated by humoral factors...
  63. ncbi AMPK expression and phosphorylation are increased in rodent muscle after chronic leptin treatment
    Gregory R Steinberg
    Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
    Am J Physiol Endocrinol Metab 284:E648-54. 2003
    ..However, taken as a whole, our data support recent evidence in rodent muscle that leptin stimulates FA oxidation through stimulation of AMPK and a subsequent downregulation of ACC activity...
  64. ncbi Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues
    Nathan K Lebrasseur
    Diabetes and Metabolism Research Unit, Boston University School of Medicine, 650 Albany St, X 820, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 291:E175-81. 2006
    ..They suggest that this effect is associated with a change in cellular energy state and that it is not dependent on PPARgamma-mediated gene transcription...
  65. ncbi LKB1-AMPK signaling in muscle from obese insulin-resistant Zucker rats and effects of training
    Apiradee Sriwijitkamol
    Diabetes Division, University of Texas Health Science Center at San Antonio, TX, USA
    Am J Physiol Endocrinol Metab 290:E925-32. 2006
    ..54, P = 0.02), and PGC-1alpha protein content (r = 0.81, P < 0.001). In summary, obese insulin-resistant rodents have abnormalities in the LKB1-AMPK-PGC-1 pathway in muscle, and these abnormalities can be restored by training...
  66. ncbi Hyperglycemia- and hyperinsulinemia-induced alteration of adiponectin receptor expression and adiponectin effects in L6 myoblasts
    X Fang
    Department of Biology, York University, Toronto, Canada
    J Mol Endocrinol 35:465-76. 2005
    ..In summary, our results suggest that both hyperglycemia and hyperinsulinemia cause gAd resistance in rat skeletal muscle cells. However, hyperinsulinemia induces a switch toward increased fAd sensitivity in these cells...
  67. ncbi Chronic activation of PPARalpha is detrimental to cardiac recovery after ischemia
    Nandakumar Sambandam
    Department of Pediatrics and Pharmacology, Univ of Alberta, Edmonton, AB, Canada
    Am J Physiol Heart Circ Physiol 290:H87-95. 2006
    ..75 +/- 9%). This study demonstrates that chronic activation of PPARalpha is detrimental to the cardiac recovery during reperfusion after ischemia...
  68. ncbi AMPK activation is not critical in the regulation of muscle FA uptake and oxidation during low-intensity muscle contraction
    Marcella A Raney
    Dept of Kinesiology and Biological Sciences, Diabetes Research Center, Univ of Southern California, 3560 Watt Way, PED 107, Los Angeles, CA 90089 0652, USA
    Am J Physiol Endocrinol Metab 288:E592-8. 2005
    ..These results suggest that while AMPK-dependent mechanisms may regulate FA uptake and FA oxidation at rest, AMPK-independent mechanisms predominate during low-intensity muscle contraction...
  69. ncbi AMP-activated protein kinase and coordination of hepatic fatty acid metabolism of starved/carbohydrate-refed rats
    Murwarid M Assifi
    Diabetes and Metabolism Unit, Boston Univ Medical Center, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 289:E794-800. 2005
    ..They also suggest that the changes in these enzymes, and later occurring increases in enzymes regulating fatty acid and glycerolipid synthesis, could be coordinated by AMPK...
  70. ncbi gAd-globular head domain of adiponectin increases fatty acid oxidation in newborn rabbit hearts
    Arzu Onay-Besikci
    Cardiovascular Research Group, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 279:44320-6. 2004
    ..These results suggest that a decrease in plasma insulin and increase in gAd are involved in the increase of cardiac fatty acid oxidation in the immediate newborn period...
  71. pmc A family of Acrp30/adiponectin structural and functional paralogs
    Guang W Wong
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 101:10302-7. 2004
    ..The discovery of a family of adiponectin paralogs has implications for understanding the control of energy homeostasis and could provide new targets for pharmacologic intervention in metabolic diseases such as diabetes and obesity...
  72. ncbi Skeletal muscle AMP-activated protein kinase phosphorylation parallels metabolic phenotype in leptin transgenic mice under dietary modification
    Tomohiro Tanaka
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Diabetes 54:2365-74. 2005
    ..activity in leptin-sensitive and -insensitive states, we examined phosphorylation of AMPK and its target, acetyl CoA carboxylase (ACC), in muscles from LepTg under dietary modification...
  73. ncbi Thyroid hormone activates adenosine 5'-monophosphate-activated protein kinase via intracellular calcium mobilization and activation of calcium/calmodulin-dependent protein kinase kinase-beta
    Masako Yamauchi
    Department of Endocrinology, Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Mol Endocrinol 22:893-903. 2008
    ..These results demonstrate that T3 nontranscriptionally activates AMPK via intracellular Ca2+ mobilization and CaMKKbeta activation, thereby stimulating mitochondrial fatty acid oxidation...
  74. pmc Short-term exercise training in humans reduces AMPK signalling during prolonged exercise independent of muscle glycogen
    Glenn K McConell
    Department of Physiology, University of Melbourne, Parkville, Victoria, Australia
    J Physiol 568:665-76. 2005
    ..It appears that muscle glycogen is not an important regulator of AMPK activation during exercise in humans when exercise is begun with normal or high muscle glycogen levels...
  75. ncbi Pioglitazone treatment activates AMP-activated protein kinase in rat liver and adipose tissue in vivo
    Asish K Saha
    Department of Medicine, Boston University Medical Center, Boston, MA 02118, USA
    Biochem Biophys Res Commun 314:580-5. 2004
    ..administered orally to intact rats, on AMPK phosphorylation (AMPK-P) (a measure of its activation) and acetyl CoA carboxylase (ACC) activity and malonyl CoA concentration in rat liver and adipose tissue...
  76. ncbi Prior exercise increases phosphorylation of Akt substrate of 160 kDa (AS160) in rat skeletal muscle
    Edward B Arias
    University of Michigan, Division of Kinesiology, Muscle Biology Laboratory, Ann Arbor, MI 48109 2214, USA
    Am J Physiol Endocrinol Metab 292:E1191-200. 2007
    ....
  77. ncbi Prevention of hepatic steatosis and hepatic insulin resistance in mitochondrial acyl-CoA:glycerol-sn-3-phosphate acyltransferase 1 knockout mice
    Susanne Neschen
    Howard Hughes Medical Institute, Department of Internal Medicine, Yale University School of Medicine, New Haven, CN 06520, USA
    Cell Metab 2:55-65. 2005
    ..These data suggest that mtGPAT1 might serve as a novel target for treatment of hepatic steatosis and hepatic insulin resistance and that long chain acyl-CoA's do not mediate fat-induced hepatic insulin resistance in this model...
  78. ncbi C75, a fatty acid synthase inhibitor, modulates AMP-activated protein kinase to alter neuronal energy metabolism
    Leslie E Landree
    Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:3817-27. 2004
    ..These data demonstrate that C75 modulates the levels of energy intermediates, thus, affecting the energy sensor AMPK. Similar effects in hypothalamic neurons could form the basis for the effects of C75 on feeding behavior...
  79. pmc c-Cbl-deficient mice have reduced adiposity, higher energy expenditure, and improved peripheral insulin action
    Juan C Molero
    Diabetes and Obesity Program, The Garvan Institute of Medical Research, Darlinghurst, New South Wales, Australia
    J Clin Invest 114:1326-33. 2004
    ..These findings suggest that c-Cbl plays an integral role in whole-body fuel homeostasis by regulating whole-body energy expenditure and insulin action...
  80. ncbi Mammalian AMP-activated protein kinase is homologous to yeast and plant protein kinases involved in the regulation of carbon metabolism
    D Carling
    Medical Research Council of Molecular Medicine, Royal Postgraduate Medical School, London, United Kingdom
    J Biol Chem 269:11442-8. 1994
    ..Our results suggest that AMPK may be involved in the regulation of a wide range of metabolic pathways...
  81. ncbi Regulation of fatty acid uptake and metabolism in L6 skeletal muscle cells by resistin
    Rengasamy Palanivel
    Department of Biology, York University, Toronto, Ont, Canada M3J 1P3
    FEBS Lett 579:5049-54. 2005
    ....
  82. ncbi AMP-activated protein kinase activation by adrenoceptors in L6 skeletal muscle cells: mediation by alpha1-adrenoceptors causing glucose uptake
    Dana S Hutchinson
    Department of Physiology, The Wenner Gren Institute, Stockholm University, SE 10691 Stockholm, Sweden
    Diabetes 55:682-90. 2006
    ..These results suggest a novel pathway where alpha1-adrenoceptor activation, independent of protein kinase C, leads to activation of AMPK in skeletal muscle, which contributes to alpha1-adrenoceptor-mediated increases in glucose uptake...
  83. ncbi Diphenylene iodonium stimulates glucose uptake in skeletal muscle cells through mitochondrial complex I inhibition and activation of AMP-activated protein kinase
    Dana S Hutchinson
    Department of Physiology, Arrhenius Laboratories F3, The Wenner Gren Institute, Stockholm University, 10691 Stockholm, Sweden
    Cell Signal 19:1610-20. 2007
    ..We postulate that DPI through inhibition of mitochondrial complex 1 and decreases in oxygen consumption, leading to decreases of ATP and activation of AMPK, stimulates glucose uptake in skeletal muscle cells...
  84. ncbi Acute regulation of fatty acid oxidation and amp-activated protein kinase in human umbilical vein endothelial cells
    Z Dagher
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Circ Res 88:1276-82. 2001
    ..transferase 1), which temporally parallels the increase in AMPK activity and a decrease in the activity of acetyl CoA carboxylase (ACC)...
  85. ncbi Nicotine-induced activation of AMP-activated protein kinase inhibits fatty acid synthase in 3T3L1 adipocytes: a role for oxidant stress
    Zhibo An
    Vascular Biology Laboratory, Department of Surgery, Graduate School of Medicine, University of Tennessee, Knoxville, TN 37922, USA
    J Biol Chem 282:26793-801. 2007
    ..Taken together, our results strongly suggest that nicotine, via ONOO(-) activates AMPK, resulting in enhanced threonine phosphorylation and consequent inhibition of FAS...
  86. ncbi Citrate diminishes hypothalamic acetyl-CoA carboxylase phosphorylation and modulates satiety signals and hepatic mechanisms involved in glucose homeostasis in rats
    Maristela Cesquini
    Departamento de Bioquimica, IB, Universidade Estadual de Campinas, SP, Brazil
    Life Sci 82:1262-71. 2008
    ....
  87. ncbi Ca2+/calmodulin-dependent protein kinase kinase is involved in AMP-activated protein kinase activation by alpha-lipoic acid in C2C12 myotubes
    Qingwu W Shen
    Department of Animal Science, Interdepartmental Molecular and Cellular Life Science Program, University of Wyoming, Laramie, WY 82071, USA
    Am J Physiol Cell Physiol 293:C1395-403. 2007
    ..Phosphorylation of the AMPK substrate, acetyl CoA carboxylase (ACC), at Ser(79) was also increased...
  88. ncbi LKB1 and the regulation of malonyl-CoA and fatty acid oxidation in muscle
    D M Thomson
    Dept of Physiology and Developmental Biology, Brigham Young Univ, Provo, UT 84602, USA
    Am J Physiol Endocrinol Metab 293:E1572-9. 2007
    ..Regardless, our results suggest that LKB1 is an important regulator of malonyl-CoA levels and fatty acid oxidation in skeletal muscle...
  89. ncbi Hepatic PGC-1beta overexpression induces combined hyperlipidemia and modulates the response to PPARalpha activation
    Christopher J Lelliott
    AstraZeneca R and D, Department of Integrative Pharmacology HE119, SE 431 83 Molndal, Sweden
    Arterioscler Thromb Vasc Biol 27:2707-13. 2007
    ..Here, we examined the in vivo effects of these two coactivators in potentiating the lipid lowering properties of the PPARalpha agonist Wy14,643 (Wy)...
  90. pmc Copper deficiency results in AMP-activated protein kinase activation and acetylCoA carboxylase phosphorylation in rat cerebellum
    Anna A Gybina
    Department of Biochemistry and Molecular Biology, University of Minnesota Medical School Duluth, 1035 University Drive, Duluth, MN 55812, USA
    Brain Res 1204:69-76. 2008
    ..AMPK activation may explain how ATP levels can be maintained even with a severe mitochondrial loss of CCO function...
  91. ncbi Activation of AMP-activated protein kinase, inhibition of pyruvate dehydrogenase activity, and redistribution of substrate partitioning mediate the acute insulin-sensitizing effects of troglitazone in skeletal muscle cells
    S Fediuc
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada
    J Cell Physiol 215:392-400. 2008
    ..These effects seem to be at least partially dependent on AMPK activation and may account for potential acute PPAR-gamma-independent anti-diabetic effects of thiazolidinediones in skeletal muscle...
  92. ncbi AMP-activated protein kinase phosphorylates transcription factors of the CREB family
    D M Thomson
    Dept of Physiology and Developmental Biology, 545 WIDB, Brigham Young Univ, Provo, UT 84602, USA
    J Appl Physiol (1985) 104:429-38. 2008
    ..We conclude that CREB and related proteins are direct downstream targets for AMPK and are therefore likely involved in mediating some effects of AMPK on expression of genes having a CRE in their promoters...
  93. ncbi Metabolic response to an acute jump in cardiac workload: effects on malonyl-CoA, mechanical efficiency, and fatty acid oxidation
    Lufang Zhou
    Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
    Am J Physiol Heart Circ Physiol 294:H954-60. 2008
    ....
  94. ncbi AMPK activation attenuates S6K1, 4E-BP1, and eEF2 signaling responses to high-frequency electrically stimulated skeletal muscle contractions
    David M Thomson
    Human Performance Laboratory, Department of Exercise and Sport Science, East Carolina University, Greenville, NC 27858, USA
    J Appl Physiol (1985) 104:625-32. 2008
    ..Stimulated and contralateral resting muscles were removed at 0, 20, and 40 min post-STIM, and AMPK, acetyl CoA carboxylase (ACC), Akt, eukaryotic initiation factor 4E-binding protein (4E-BP1), 70-kDa ribosomal protein S6 kinase (..
  95. ncbi Metoprolol improves cardiac function and modulates cardiac metabolism in the streptozotocin-diabetic rat
    Vijay Sharma
    Division of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Univ of British Columbia, Vancouver, Canada
    Am J Physiol Heart Circ Physiol 294:H1609-20. 2008
    ..The metabolism changes occur in parallel with changes in gene expression...
  96. ncbi AMPK and ACC phosphorylation: effect of leptin, muscle fibre type and obesity
    A Janovska
    Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia
    Mol Cell Endocrinol 284:1-10. 2008
    ..AMPK activation by leptin occurred independently of energy-related metabolites. These data demonstrate that the leptin effect on AMPKalpha and ACCbeta is muscle fibre type dependent and fails in diet-induced obesity...
  97. ncbi Carbohydrate ingestion does not alter skeletal muscle AMPK signaling during exercise in humans
    Robert S Lee-Young
    Dept of Physiology, The University of Melbourne, Carlton, Victoria, Australia
    Am J Physiol Endocrinol Metab 291:E566-73. 2006
    ....
  98. ncbi Chronic hexosamine flux stimulates fatty acid oxidation by activating AMP-activated protein kinase in adipocytes
    Bai Luo
    Division of Endocrinology, University of Utah School of Medicine and Veterans Affairs Medical Center, Salt Lake City, Utah 84132, USA
    J Biol Chem 282:7172-80. 2007
    ..Moreover, removal of O-GlcNAc by hexosaminidase reduced AMPK activity. We conclude that chronically high hexosamine flux stimulates fatty acid oxidation by activating AMPK in adipocytes, in part through O-linked glycosylation...
  99. ncbi Regulation of fatty acid oxidation and glucose metabolism in rat soleus muscle: effects of AICAR
    V K Kaushik
    Diabetes Unit, Section of Endocrinology, Boston University Medical School, Boston, Massachusetts 02118, USA
    Am J Physiol Endocrinol Metab 281:E335-40. 2001
    ....
  100. ncbi Malonyl CoA control of fatty acid oxidation in the ischemic heart
    Jason R B Dyck
    Cardiovascular Research Group, Departments of Pediatrics and Pharmacology, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Mol Cell Cardiol 34:1099-109. 2002
    ..The levels of malonyl CoA are regulated both by its synthesis by acetyl CoA carboxylase (ACC) and its degradation by malonyl CoA decarboxylase (MCD)...
  101. ncbi Modulation of fatty acid metabolism as a potential approach to the treatment of obesity and the metabolic syndrome
    Jun Kusunoki
    Department of Metabolic Disorders, Banyu Tsukuba Research Institute, Tsukuba, Japan
    Endocrine 29:91-100. 2006
    ..Inhibition of hormone-sensitive lipase is another possible approach. The rationale for modulating the activity of these enzymes and their relative merits (and downsides) as possible therapeutic targets are further discussed...

Research Grants62

  1. Intermediary Metabolism, Histone Acetylation, and Transcriptional Regulation
    Ales Vancura; Fiscal Year: 2013
    ..In addition, this project will provide an excellent research environment for undergraduate and graduate students, as they prepare for careers in biomedical research. ..
  2. Lipid Transport, Bile Acid Synthesis, and Cholesterol Homeostasis
    William M Pandak; Fiscal Year: 2013
    ..of key nuclear receptors important in lipid homeostasis, and reduced expression of HMG CoA reductase, acetyl CoA carboxylase, and fatty acid synthase (rate-determining enzymes in the biosynthesis of cholesterol and fatty acids)...
  3. Mechanisms of diet-induced leptin resistance in ARC and VTA
    Philip J Scarpace; Fiscal Year: 2013
    ..and activator of transcription protein 3 (STAT3) and the nutrient sensing AMP-activated kinase (AMPK)/acetyl CoA carboxylase (ACC) pathways. Leptin signals through the latter by promoting dephosphorylation of pAMPK/pACC...
  4. Smooth muscle cell lipid metabolism and serum amyloid A
    Barbara M Schreiber; Fiscal Year: 2010
    ..Moreover, SAA down-regulates expression of the known SREBP-regulated gene acetyl CoA carboxylase. Interestingly, unlike other agents that traffic cholesterol to the ER, SAA mediates this effect without ..
  5. REGULATION OF 15N UREA ISOTOPOMERS PRODUCTION
    Itzhak Nissim; Fiscal Year: 2009
    ..and disease states and thus may lead to development of a novel intervention to improve urea synthesis and detoxification of ammonia associated with fatty liver or in cases such as drug-induced inhibition of urea synthesis ..
  6. Cardiac metabolism and heart failure
    Rong Tian; Fiscal Year: 2010
    ..Results from the study will provide a basis for optimizing cardiac metabolism as part of the treatment strategy for heart failure. This is particularly important for patients with metabolic disorders such as obesity or diabetes. ..
  7. An investigation of the mechanisms by which down-regulation of lipoprotein lipase
    Jheem D Medh; Fiscal Year: 2013
    ..in the expression pattern of various genes involved in insulin signaling including Glut4, PI3Kinase, acetyl coA carboxylase, etc...
  8. FINR: A Novel Modulator of Insulin Signaling
    Marc R Montminy; Fiscal Year: 2010
    ..In recent studies, we found that TRB3 promotes fatty acid oxidation in adipose by associating with acetyl coA carboxylase (ACC), the rate limiting enzyme in fatty acid synthesis...
  9. Sir2 Regulates AMPK and Lipid Metabolism in Diabetes
    Mengwei Zang; Fiscal Year: 2012
    ..Activation of SIRT1/AMPK signaling will provide new therapeutic targets for intervention of diabetes and its vascular complications. ..
  10. Regulation of lipogenesis by lysophosphatidic acid in ovarian cancer
    Xianjun Fang; Fiscal Year: 2012
    ..This study will answer whether LPA is a pathophysiological determinant of the hyperactive lipogenesis in ovarian cancer. The results could be translated into a novel and innovative approach to targeting tumor-specific lipogenesis. ..
  11. ENGINEERED GLUCOSE METABOLISM IN INSULIN SECRETING CELLS
    Christopher B Newgard; Fiscal Year: 2010
    ..of metabolic function that link glucose and lipid metabolism in beta-cells, including citrate lyase, acetyl CoA carboxylase-1 (ACC-1), and the GPR40 receptor...
  12. Causative role of lipid peroxidation products in obesity and aging
    Sharda P Singh; Fiscal Year: 2013
    ..Future interventions based on the knowledge to be gained may delay the onset of these conditions. ..
  13. Treatment of Cytomegalovirus by Inhibition of Host Lipid Synthesis
    Bryson D Bennett; Fiscal Year: 2012
    ..The present project proposes to demonstrate proof-of-concept efficacy of host cell lipid metabolism as a novel target for anti-CMV therapy. ..
  14. The fuel sensing enzyme AMPK in the pathogenesis of prostate cancer
    Zhijun Luo; Fiscal Year: 2010
    ..We have also found that these effects are associated with inhibition of fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and mTOR...
  15. Metabolic regulatory mechanisms essential for Human Cytomegalovirus replication
    JOSHUA C MUNGER; Fiscal Year: 2013
    ..Elucidating these mechanisms will present targets to therapeutically block viral replication and attenuate HCMV-associated disease. ..
  16. Metabolic Pathways Involved in the CNS Regulation of Energy Balance
    MARGARET STEFATER; Fiscal Year: 2012
    ..This contribution is significant, because an understanding of central fuel sensing will identify novel therapeutic strategies for the treatment and/or prevention of obesity. ..
  17. Mechanisms and consequences of metabolic manipulation by human cytomegalovirus
    Thomas E Shenk; Fiscal Year: 2013
    ..These studies address an important and understudied area of HCMV biology, they will advance fundamental understanding of metabolic regulation, and they will lay the foundation for the discovery of novel anti-virals. ..
  18. QUANTITATIVE STUDIES OF THE BIOTIN REGULATORY SYSTEM
    Dorothy Beckett; Fiscal Year: 2010
    ..BirA funnels biotin into metabolism by catalyzing its linkage to the biotin-dependent carboxylase, acetyl CoA carboxylase, and binds sequence specifically to the biotin operator sequence to regulate transcription of the biotin ..
  19. Postnatal Actions of Maternal Obesity on Neonatal Metabolic Health
    JAMES LEWIS MCMANAMAN; Fiscal Year: 2013
    ....
  20. Controlling Fatty Acid Synthesis in African Trypanosomes
    KIMBERLY SUE PAUL; Fiscal Year: 2010
    ....
  21. DIETARY OMEGA-3 FATTY ACIDS, GENE EXPRESSION AND GROWTH
    William Heird; Fiscal Year: 2001
    ..abstract_text> ..
  22. Regulation of energy metabolism in the failing heart
    Raymond Russell; Fiscal Year: 2005
    ..The findings of these studies will help to characterize the defects in energy metabolism in the failing heart and aid in the design of therapies that improve energy transduction in the failing human heart. ..
  23. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ..For example, acetyl CoA carboxylase (ACC), a lipogenic enzyme, is modulated by phosphorylation (inactive)- dephosphorylation (active)...
  24. Role of Protein (S14)in Normal and Neoplastic Mammary
    WILLIAM KINLAW; Fiscal Year: 2005
    ..These studies will provide a comprehensive view of the role of this novel nuclear protein in both normal mammary physiology and in breast cancer, and are likely to reveal a new class of therapeutic targets. ..
  25. Structure-Function Analysis of Enzymes
    Hazel Holden; Fiscal Year: 2004
    ..systems known to employ substrate channeling: carbamoyl phosphate synthetase, asparagine synthetase, and acetyl CoA carboxylase. The first two enzymes utilize substrate channeling between active sites within a single polypeptide chain ..
  26. FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
    DIANE HICKSON BICK; Fiscal Year: 2002
    ..Location of a malonyl-CoA compartment that is inaccessible to CPT-I will shed light on the inconsistency between the high malonyl CoA content in heart and reliance on beta-oxidation. ..
  27. Therapeutics targeting fatty acid synthesis in P. aeruginosa
    Donald Moir; Fiscal Year: 2009
    ..Inhibitors will be verified to act on this pathway by testing them in a biochemical assay of the entire fatty acid synthesis pathway. ..
  28. CHOLESTEROL REGULATION BY CEREALS AND FUNGAL PRODUCTS
    WARREN BURGER; Fiscal Year: 1980
    ..The pronounced effects observed by us which are detailed in this proposal have implications for human nutrition and possible control of cardiovascular diseases in which cholesterol plays a key role...
  29. PUTATIVE MEDIATORS OF INSULIN SECRETION
    SUZANNE LAYCHOCK; Fiscal Year: 2000
    ....
  30. REGULATION OF LIPOGENESIS BY THYROID HORMONES AND DIET
    Hedley Freake; Fiscal Year: 1993
    ..Simple purification of these proteins will be attempted and their ability to stimulate RNA synthesis in an in vitro transcriptional system assessed...
  31. HORMONE REGULATION & STRUCTURE OF ACETYL-COA CARBOXYLASE
    SALIH WAKIL; Fiscal Year: 1990
    ..The cDNA will be used in determining the amino acid sequence of the carboxylase subunit and in the isolation and characterization of the carboxylase gene...
  32. REGULATION OF LIPOGENESIS BY POLYUNSATURATED FATS
    Steven Clarke; Fiscal Year: 1980
    ..In contrast to the rat, PUFA and SFA inhibit chick liver lipogenesis with similar efficacy...
  33. LIPOGENESIS IN TPN-INDUCED HEPATIC STEATOSIS
    Michael Narkewicz; Fiscal Year: 1991
    ..By defining the role of lipogenesis and lipid transport in the hepatic steatosis TPN, appropriate hormonal or nutritional therapy may be designed to prevent this complication...
  34. CATALYTIC MECHANISM OF BIOTIN DEPENDENT ENZYMES
    Grover Waldrop; Fiscal Year: 2001
    DESCRIPTION: The long term objective of this research is to understand the catalytic mechanism of acetyl CoA carboxylase from E. coli...
  35. PURCHASE OF AN INTERACTIVE LASER CYTOMETER
    Randolph Noelle; Fiscal Year: 1993
    ..by antiviral CTL, 11) the regulation of ion channel distribution at synapses, 12) the regulation of acetyl CoA carboxylase in hepatoma cells, 13) the selection of clones stably expressing androgen receptor and 14) studies into ..
  36. Roles and Regulation of Acetyl-CoA Carboxylase
    LEE WITTERS; Fiscal Year: 2006
    ..Our overall emphasis, building knowledge of the structure/function of the complex AMPK enzyme, is necessary to understand its important roles in cellular metabolic regulation. ..
  37. EXERCISE AND SKELETAL MUSCLE FATTY ACID METABOLISM
    LORRAINE TURCOTTE; Fiscal Year: 2002
    ..For the first time, malonyl-CoA levels will be correlated to the rate of fatty acid oxidation in skeletal muscle. ..
  38. EXERCISE, LIPOLYSIS AND FAT OXIDATION
    Robert Wolfe; Fiscal Year: 2002
    ..abstract_text> ..
  39. REGULATION OF CARDIAC SUBSTRATE METABOLISM DURING STRESS
    William Stanley; Fiscal Year: 2001
    ..CPT-I is inhibited by malonyl CoA, which is synthesized in the cytosol by acetyl CoA carboxylase (ACC)...
  40. REGULATION OF CARDIAC SUBSTRATE METABOLISM DURING STRESS
    William Stanley; Fiscal Year: 2002
    ..CPT-I is inhibited by malonyl CoA, which is synthesized in the cytosol by acetyl CoA carboxylase (ACC)...
  41. GENETIC REGULATION OF FATTY ACID BIOSYNTHESIS IN YEAST
    SUBRAHMANYAM CHIRALA; Fiscal Year: 1992
    ..Isolation of regulatory factors and characterization of the corepressor will help us understand the fatty-acid-mediated repression of these genes...
  42. INSULIN ACTION ON PROTEIN KINASE ACTIVITY
    Michael Czech; Fiscal Year: 1990
    ..Several typical insulin sensitive phosphoproteins such as the insulin receptor, acetyl CoA carboxylase and ATP citrate lyase will be evaluated as potential substrates for the kinase...
  43. DRUG DISCOVERY FOR TREATMENT OF OBESITY
    Ilya Okun; Fiscal Year: 2004
    ..on ADME criteria for the likelihood that they will exhibit specific inhibitory activity against mammalian acetyl CoA carboxylase 2 (ACC2). ACC2 is found in mitochondria, where it regulates fatty acid oxidation...
  44. Metabolic Control by novel Acrp30-like proteins
    GUANG WONG; Fiscal Year: 2006
    ..oligomeric states of Acrp30 activate NF-kappaB or induce phosphorylation of AMP-activated kinase and acetyl CoA carboxylase, I will examine if similar signaling pathways are also activated by CTRP2 and CTRP7...
  45. IDENTIFICATION OF NEW ACETYL-COA CARBOXYLASE INHIBITORS
    TEDD ELICH; Fiscal Year: 2004
    b>Acetyl CoA carboxylase catalyzes the first step in fatty acid biosynthesis and is a key regulator of fat metabolism...
  46. Discovery of Small Molecule hACC2 Inhibitors for Treatment of Obesity
    Ilya Okun; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  47. MEDIATION OF INSULIN ACTION
    Leonard Jarett; Fiscal Year: 1993
    ..regulate insulin-sensitive enzymes and, in particular, the low Km cyclic AMP phosphodiesterase and acetyl CoA carboxylase. The former enzyme may not be regulated by phosphorylation while the latter enzyme has its activity ..
  48. Regulation and function of SREBP-1c in skeletal muscle
    MICHAEL BIZEAU; Fiscal Year: 2004
    ..This research experience will allow the candidate to compete for R01 funding and establish an independent research career. ..
  49. Causes and consequences of AMPK activation in the heart
    JAMES BALSCHI; Fiscal Year: 2005
    ..abstract_text> ..
  50. INSULIN INDUCTION OF LIPOGENIC ENZYMES IN HEPATOCYTES
    David Gibson; Fiscal Year: 1980
    ..and change in concentration of the inactive (phosphorylated) and active (dephosphorylated) forms of acetyl CoA carboxylase and other enzymes as a function of preinduction time...
  51. Regulation of Skeletal Muscle LKB1
    WILLIAM WINDER; Fiscal Year: 2010
    ..The proposed studies will provide additional rationale for convincing a predominantly sedentary population of the importance of regular exercise in combating inactivity-related diseases. ..
  52. Antiadipogenic Influence of HIV Protease Inhibitors
    M Lane; Fiscal Year: 2005
    ..The long term objective of the proposed research is to facilitate the design of anti-HIV therapeutics that lack lipoatrophic potential. ..
  53. REGULATION OF LIPOGENESIS
    M Lane; Fiscal Year: 2001
    ..for derepression and repression of the SCD1 and SCD2 genes. ..
  54. FACTORS AND GENES RESPONSIBLE FOR ADIPOCYTE COMMITMENT
    M Lane; Fiscal Year: 2006
    ..abstract_text> ..
  55. Physiology of Malonyl-CoA in Muscle during Exercise
    WILLIAM WINDER; Fiscal Year: 2006
    ..The results will provide additional rationale for the use of regular exercise in preventing and treating type 2 diabetes and obesity. ..
  56. HORMONAL CONTROL OF ADIPOSE GENE EXPRESSION
    M Lane; Fiscal Year: 2007
    ..The 32kDa CUP/AP-2alpha- interacting protein, p32, previously identified in our lab, will be characterized and its role determined. ..
  57. IDENTIFICATION OF NEW ACETYL-COA CARBOXYLASE INHIBITORS
    STEVEN BREAZEALE; Fiscal Year: 2008
    ..Furthermore, soraphen is not a good starting point for drug development due to its complex structure. We propose to identify novel small molecules that target the soraphen binding ..
  58. Postsynthetic Modifications of Bacterial Membrane Lipids
    John Cronan; Fiscal Year: 2006
    ..These processes will be studied by a combination of biochemical, genetic, and molecular biological approaches including tests of pathogenesis in a mouse model system ..