muramoylpentapeptide carboxypeptidase

Summary

Summary: Enzyme which catalyzes the peptide cross-linking of nascent CELL WALL; PEPTIDOGLYCAN.

Top Publications

  1. ncbi Penicillin-binding proteins as resistance determinants in clinical isolates of Streptococcus pneumoniae
    P Reichmann
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Microb Drug Resist 2:177-81. 1996
  2. ncbi A surface plasmon resonance analysis of the interaction between the antibiotic moenomycin A and penicillin-binding protein 1b
    Katherina Stembera
    Institut fur Organische Chemie, Universitat Leipzig, Johannisallee 29, 04103 Leipzig, Germany
    Chembiochem 3:559-65. 2002
  3. ncbi beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
  4. pmc Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis
    B G Spratt
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
    Proc Natl Acad Sci U S A 86:8988-92. 1989
  5. ncbi Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
    Reiko Yoshida
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113, Japan
    J Antimicrob Chemother 51:247-55. 2003
  6. pmc Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test
    Annie Felten
    Service de Bactériologie Virologie Hygiène, Hopital Saint Louis, 75475 Paris Cedex 10, France
    J Clin Microbiol 40:2766-71. 2002
  7. ncbi TAK-599, a novel N-phosphono type prodrug of anti-MRSA cephalosporin T-91825: synthesis, physicochemical and pharmacological properties
    Tomoyasu Ishikawa
    Pharmaceutical Research Division, Takeda Chemical Industries, Ltd, 2 17 85 Jusohonmachi, Yodogawa ku, 532 8686, Osaka, Japan
    Bioorg Med Chem 11:2427-37. 2003
  8. ncbi Evaluation of different primers for detecting mecA gene by PCR in comparison with phenotypic methods for discrimination of methicillin-resistant Staphylococcus aureus
    Taweeporn Siripornmongcolchai
    Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
    Southeast Asian J Trop Med Public Health 33:758-63. 2002
  9. ncbi Penicillin-binding proteins involved in high-level piperacillin resistance in Veillonella spp
    Maria M Theron
    Department of Medical Microbiology G4, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa
    J Antimicrob Chemother 52:120-2. 2003
  10. ncbi First report of a catalase-negative methicillin-resistant Staphalococcus aureus
    Xavier Bertrand
    Service d Hygiène Hospitalière et d Epidémiologie moléculaire CHU, Besançon 25030, France
    Diagn Microbiol Infect Dis 43:245-6. 2002

Research Grants

  1. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
  2. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
  3. Acquisition of a 600 MHz NMR Spectrometer - Wayne State
    Shahriar Mobashery; Fiscal Year: 2002
  4. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
  5. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2010
  6. RIBOSOMAL FUNCTION & ANTIBIOTIC DESIGN
    Shahriar Mobashery; Fiscal Year: 2007
  7. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
  8. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
  9. ASSEMBLY OF THE DIVISION SEPTUM IN ESCHERICHIA COLI
    David Weiss; Fiscal Year: 2003
  10. PENICILLIN INTERACTIVE PROTEINS OF STAPHYLOCOCCUS AUREUS
    Henry Chambers; Fiscal Year: 2003

Detail Information

Publications189 found, 100 shown here

  1. ncbi Penicillin-binding proteins as resistance determinants in clinical isolates of Streptococcus pneumoniae
    P Reichmann
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Microb Drug Resist 2:177-81. 1996
    ..In contrast, high-level cefotaxime resistance appeared to be due to a low affinity PBP2a, indicating that this PBP can also function as a resistance determinant...
  2. ncbi A surface plasmon resonance analysis of the interaction between the antibiotic moenomycin A and penicillin-binding protein 1b
    Katherina Stembera
    Institut fur Organische Chemie, Universitat Leipzig, Johannisallee 29, 04103 Leipzig, Germany
    Chembiochem 3:559-65. 2002
    ..The method seems to be appropriate for use in screens for transglycosylase inhibitors that bind to the moenomycin-binding site of the enzyme...
  3. ncbi beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
    ....
  4. pmc Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis
    B G Spratt
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
    Proc Natl Acad Sci U S A 86:8988-92. 1989
    ..flavescens, or a very similar commensal Neisseria species, appears to have occurred independently during the development of altered penA genes in non-beta-lactamase-producing penicillin-resistant strains of N. gonorrhoeae...
  5. ncbi Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
    Reiko Yoshida
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113, Japan
    J Antimicrob Chemother 51:247-55. 2003
    ..The strain was characteristically a non-producer of autolytic enzyme, though the phenotype, which was lost easily, was not directly correlated with methicillin resistance...
  6. pmc Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test
    Annie Felten
    Service de Bactériologie Virologie Hygiène, Hopital Saint Louis, 75475 Paris Cedex 10, France
    J Clin Microbiol 40:2766-71. 2002
    ..Therefore, the cefoxitin and moxalactam disk diffusion methods were the best-performing tests for routine detection of all classes of MRSA...
  7. ncbi TAK-599, a novel N-phosphono type prodrug of anti-MRSA cephalosporin T-91825: synthesis, physicochemical and pharmacological properties
    Tomoyasu Ishikawa
    Pharmaceutical Research Division, Takeda Chemical Industries, Ltd, 2 17 85 Jusohonmachi, Yodogawa ku, 532 8686, Osaka, Japan
    Bioorg Med Chem 11:2427-37. 2003
    ..These results show that 1 (TAK-599) is a highly promising parenteral cephalosporin targeted for MRSA infection...
  8. ncbi Evaluation of different primers for detecting mecA gene by PCR in comparison with phenotypic methods for discrimination of methicillin-resistant Staphylococcus aureus
    Taweeporn Siripornmongcolchai
    Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
    Southeast Asian J Trop Med Public Health 33:758-63. 2002
    ..There was a good correlation among the mecA gene detection, ODD and OSS methods. The discrepancy of three isolates between PCR and phenotypic methods should be clarified for other resistant mechanisms...
  9. ncbi Penicillin-binding proteins involved in high-level piperacillin resistance in Veillonella spp
    Maria M Theron
    Department of Medical Microbiology G4, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa
    J Antimicrob Chemother 52:120-2. 2003
    ..To investigate high-level piperacillin resistance in Veillonella spp. in the absence of beta-lactamase activity...
  10. ncbi First report of a catalase-negative methicillin-resistant Staphalococcus aureus
    Xavier Bertrand
    Service d Hygiène Hospitalière et d Epidémiologie moléculaire CHU, Besançon 25030, France
    Diagn Microbiol Infect Dis 43:245-6. 2002
    ..The isolated strain had phenotypic and genotypic characteristics of the major epidemic clone spreading in our region with the exception that catalase activity could not be found...
  11. ncbi Inducibility and potential role of MecA-gene-positive oxacillin-susceptible Staphylococcus aureus from colonized healthcare workers as a source for nosocomial infections
    G Kampf
    Institute of Hygiene, University Hospital Benjamin Franklin, Free University Berlin, Berlin, Germany
    J Hosp Infect 54:124-9. 2003
    ..It might be useful to screen methicillin-susceptible S. aureus isolates from HCWs for the mecA gene when recurrent infections with MRSA occur on a ward and a source cannot be found...
  12. ncbi Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin
    A Antignac
    , Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Antimicrob Chemother 47:285-96. 2001
    ....
  13. ncbi [Comparison of methods used to detect methicillin resistance in staphylococci]
    Cigdem Kuzucu
    Inonu Universitesi Tip Fakultesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Malatya
    Mikrobiyol Bul 36:253-7. 2002
    ..aureus strains, but oxacillin agar screen revealed to be unsatisfactory for the detection of methicillin resistance in CNS...
  14. pmc In vitro activity of S-3578, a new broad-spectrum cephalosporin active against methicillin-resistant staphylococci
    Takaji Fujimura
    Discovery Research Laboratories, Shionogi and Co, Ltd, Toyonaka, Osaka 561 0825, Japan
    Antimicrob Agents Chemother 47:923-31. 2003
    ..Thus, S-3578 was considered to be worthy of further evaluation...
  15. pmc Rapid detection of methicillin resistance in coagulase-negative Staphylococci with the VITEK 2 system
    Matthias A Horstkotte
    Institut fur Medizinische Mikrobiologie und Immunologie, Universitatsklinikum Hamburg Eppendorf, D 20246 Hamburg, Germany
    J Clin Microbiol 40:3291-5. 2002
    ..The results of our study encourage the use of the VITEK 2 system, which proved to be a highly reliable and rapid phenotypic method for the detection of methicillin resistance in CoNS...
  16. ncbi Detection of intrinsic oxacillin resistance in non-multiresistant, oxacillin-resistant Staphylococcus aureus (NORSA)
    Iain B Gosbell
    J Antimicrob Chemother 51:468-70. 2003
  17. ncbi [Comparison of routine sensitivity tests and mecA gene analysis for determination of methicillin resistance in Staphylococcus aureus isolates]
    Tuba Atay
    Dokuz Eylul Universitesi Tip Fakultesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir
    Mikrobiyol Bul 36:133-40. 2002
    ..In conclusion, susceptibility tests, when performed according to NCCLS recommendations, are found to be reliable and decisive, for the detection of methicillin resistance of S. aureus...
  18. ncbi Survey of methicillin-resistant coagulase-negative staphylococci isolated from the fingers of nursing students
    Takashi Kitao
    Department of Medical Laboratory Technology, Ehime College of Health Science, 543 Takooda, Tobecho, Iyo gun, Ehime 791 2101, Japan
    J Infect Chemother 9:30-4. 2003
    ..The present results show that basic preventive measures for cross-infection should be considered in the future, using such genetic analysis methods, so that MRCNS may not cause hospital infection...
  19. ncbi Mueller-Hinton agar is superior to PDM blood agar for detection of methicillin-resistant Staphylococcus aureus
    T Monsen
    Department of Clinical Bacteriology, University Hospital of Umea, S 901 85 Umea, Sweden
    Clin Microbiol Infect 9:61-4. 2003
    ..05). Irrespective of the method of susceptibility testing evaluated, MH NaCl agar was superior to PDM blood agar for the detection of oxacillin resistance in mecA-containing S. aureus...
  20. ncbi Genetic basis of neonatal methicillin-resistant Staphylococcus aureus in Japan
    Ken Kikuchi
    Department of Infectious Diseases, Tokyo Women s Medical University, Tokyo, Japan
    Pediatr Int 45:223-9. 2003
    ..The environment of NICU and infection control intervention should be improved and a new strategy for control like vaccination or probiotics is required...
  21. pmc mecA gene is widely disseminated in Staphylococcus aureus population
    C L C Wielders
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands
    J Clin Microbiol 40:3970-5. 2002
    ..This supports the hypothesis that horizontal transfer plays an important role in the dissemination of the mecA gene in the S. aureus population...
  22. pmc Detection of methicillin and mupirocin resistance in Staphylococcus aureus isolates using conventional and molecular methods: a descriptive study from a burns unit with high prevalence of MRSA
    P U Krishnan
    Department of Clinical Microbiology, University College London Hospitals and PHLS Collaborating Centre, Grafton Way, III Floor, London W1E 6DB, UK
    J Clin Pathol 55:745-8. 2002
    ..To compare conventional phenotypic methods for the detection of methicillin and mupirocin resistance in Staphylococcus aureus in routine laboratory practice with reference to an established molecular method...
  23. ncbi Identification of a fmtA-like gene that has similarity to other PBPs and beta-lactamases in Staphylococcus aureus
    H Komatsuzawa
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    FEMS Microbiol Lett 188:35-9. 2000
    ..Recombinant FLP protein, however, lacks penicillin binding activity, and the inactivation of flp in two methicillin-resistant S. aureus strains did not cause a reduction in the methicillin resistance...
  24. ncbi Detection of mecA, mecR1 and mecI genes among clinical isolates of methicillin-resistant staphylococci by combined polymerase chain reactions
    E Petinaki
    Department of Microbiology, School of Medicine, University of Patras, 26500 Rion, Patras, Greece
    J Antimicrob Chemother 47:297-304. 2001
    ..Among methicillin-resistant S. aureus, a genetically heterogeneous population was identified, with several alterations and deletions of mec genes...
  25. ncbi Agar diffusion, agar dilution, Etest, and agar screening test in the detection of methicillin resistance in staphylococci
    Fabiana Rowe
    Hospital Mae de Deus, Porto Alegre, Brazil
    Diagn Microbiol Infect Dis 43:45-8. 2002
    ..For other species, all methods and conditions presented low specificity (ranging from 20% to 66.7%) and, particularly S. saprophyticus, may need molecular methods to correctly assess methicillin resistance...
  26. pmc Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay
    Hong Fang
    Department of Clinical Bacteriology, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    J Clin Microbiol 41:2894-9. 2003
    ..aureus were also tested by this method, which was proved to be a valuable tool for MRSA identification in a routine microbiological laboratory...
  27. ncbi The emergence and evolution of methicillin-resistant Staphylococcus aureus
    K Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo Bunkyo ku, Tokyo 113 8421, Japan
    Trends Microbiol 9:486-93. 2001
    ..aureus strains from the normal human flora into MRSA...
  28. pmc Genetic and molecular characterization of beta-lactamase-negative ampicillin-resistant Haemophilus influenzae with unusually high resistance to ampicillin
    Frank S Kaczmarek
    Department of Immunology and Infectious Disease, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    Antimicrob Agents Chemother 48:1630-9. 2004
    ..67 micro g/ml, typical for BLNAR strains. These studies illustrate that BLNAR strains with high ampicillin MICs exist that have combined resistance mechanisms in PBP3 and in the AcrAB efflux pump...
  29. ncbi Molecular characterization of penicillin non-susceptible Streptococcus pneumoniae in Christchurch, New Zealand
    David C Bean
    Centre for Infectious Disease, Barts and the London School of Medicine and Dentistry, Queen Mary College, University of London, London E1 2AD, UK
    J Antimicrob Chemother 54:122-9. 2004
    ..To determine the epidemiological relationship between non-invasive penicillin non-susceptible Streptococcus pneumoniae isolates collected in the Christchurch community between 1997 and 2001...
  30. ncbi [Methicillin resistance detection in Staphylococcus aureus: comparison between conventional methods and MRSA-Screen latex agglutination technique]
    R Soloaga
    Maestría en Microbiología Clínica, Pontificia Universidad Católica Argentina, Buenos Aires
    Rev Argent Microbiol 36:36-40. 2004
    ..All methods presented high sensitivity and specificity, but MRSA-Screen latex had the advantage of giving a reliable result, equivalent to PCR, in only 15 minutes...
  31. pmc Role of penicillin-binding protein 4 in expression of vancomycin resistance among clinical isolates of oxacillin-resistant Staphylococcus aureus
    J E Finan
    Department of Medicine, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia, USA
    Antimicrob Agents Chemother 45:3070-5. 2001
    ..These data suggest that alterations in PBP4 expression are at least partially responsible for the VISA phenotype...
  32. pmc In vitro and in vivo activities of a novel cephalosporin, BMS-247243, against methicillin-resistant and -susceptible staphylococci
    Joan C Fung-Tomc
    Department of Microbiology, Bristol Myers Squibb Co, Wallingford, Connecticut 06492, USA
    Antimicrob Agents Chemother 46:971-6. 2002
    ..In conclusion, BMS-247243 has in vitro and in vivo activities against methicillin-resistant staphylococci and thus may prove to be useful in the treatment of infections caused by these multidrug-resistant organisms...
  33. ncbi Nasopharyngeal carriage of drug-resistant Streptococcus pneumoniae in children with acute otitis media evaluated by polymerase chain reaction-based genotyping of penicillin-binding proteins
    Muneki Hotomi
    Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Japan
    Acta Otolaryngol 122:72-7. 2002
    ..Strains with mutations in the three PBP genes were often isolated from children < or = 2 years old. Evaluation of mutations in PBP genes using PCR will prove useful for studying the epidemiology of antibiotic resistance...
  34. pmc Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases
    Angela M Kearns
    Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, United Kingdom
    J Clin Microbiol 40:682-4. 2002
    ..pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases...
  35. ncbi A comparison of PCR detection of mecA with two standard methods of oxacillin disk susceptibility testing for coagulase-negative staphylococci
    Thean Y Tan
    Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff
    J Med Microbiol 51:83-5. 2002
    ..In this study, the clinical utility of mecA detection in CNS for the determination of methicillin resistance appears to be limited...
  36. pmc Differential expression of methicillin resistance by different biofilm-negative Staphylococcus epidermidis transposon mutant classes
    Dietrich Mack
    Institut fur Medizinische Mikrobiologie und Immunologie, Universitatsklinikum Hamburg Eppendorf, 20246 Hamburg, Federal Republic of Germany
    Antimicrob Agents Chemother 46:178-83. 2002
    ....
  37. pmc Effects of amino acid alterations in penicillin-binding proteins (PBPs) 1a, 2b, and 2x on PBP affinities of penicillin, ampicillin, amoxicillin, cefditoren, cefuroxime, cefprozil, and cefaclor in 18 clinical isolates of penicillin-susceptible, -intermedia
    Kensuke Nagai
    Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania, USA
    Antimicrob Agents Chemother 46:1273-80. 2002
    ..Overall, the MICs for and PBP affinities of the strains correlated with the changes found in the PBP active binding sites...
  38. pmc In vitro activities of piperacillin against beta-lactamase-negative ampicillin-resistant Haemophilus influenzae
    Yoshiro Morikawa
    Yodogawa Christian Hospital, Higashiyodogawa ku, Osaka, Japan
    Antimicrob Agents Chemother 48:1229-34. 2004
    ....
  39. pmc Mechanism of action of NB2001 and NB2030, novel antibacterial agents activated by beta-lactamases
    Geoffrey W Stone
    NewBiotics, Inc, San Diego, California 92121, USA
    Antimicrob Agents Chemother 48:477-83. 2004
    ..aureus and E. coli. Taken together, the results suggest that NB2001 and NB2030 act primarily as triclosan prodrugs in S. aureus and E. coli...
  40. ncbi Studies on anti-MRSA parenteral cephalosporins. IV. A novel water-soluble N-phosphono type prodrug for parental administration
    T Ishikawa
    Pharmaceutical Research Division, Takeda Chemical Industries, Ltd, Osaka, Japan
    J Antibiot (Tokyo) 54:364-74. 2001
    ....
  41. ncbi Methicillin-resistant Staphylococcus aureus in the hospitals of central Greece
    E Petinaki
    Department of Microbiology, School of Medicine, University of Thessaly, Larissa, Greece
    Int J Antimicrob Agents 18:61-5. 2001
    ..Misclassifications of isolates exhibiting low-level resistance (oxacillin MIC 2-4 mg/L) occurred with the salt agar screen, the oxacillin disk diffusion and the ATB Staph System methods...
  42. pmc Methicillin resistance in Staphylococcus aureus: mechanisms and modulation
    Paul D Stapleton
    School of Pharmacy, 29 39 Brunswick Square, London WC1N 1AX
    Sci Prog 85:57-72. 2002
    ....
  43. ncbi [Comparison of different detection methods for methicillin resistance in Staphylococcus aureus]
    R Soloaga
    Hospital de Ninos Ricardo Gutierrez, Buenos Aires, Argentina
    Rev Argent Microbiol 34:52-6. 2002
    ..According to our results the best performance was obtained with MOCP. However, as a rapid method, CR gave acceptable sensitivity for clinical purposes...
  44. ncbi Moenomycin-resistance is associated with vancomycin-intermediate susceptibility in Staphylococcus aureus
    Hiromi Nishi
    Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi, Hiroshima, Japan
    Microbiol Immunol 47:927-35. 2003
    ..These results indicate that the moenomycin-resistance is closely associated with vancomycin-intermediate susceptibility in S. aureus...
  45. ncbi Mechanisms of action of corilagin and tellimagrandin I that remarkably potentiate the activity of beta-lactams against methicillin-resistant Staphylococcus aureus
    Sumiko Shiota
    Department of Pathogenic Microbiology, School of Pharmacy, Shujitsu University, Okayama, Japan
    Microbiol Immunol 48:67-73. 2004
    ..Corilagin or tellimagrandin I suppressed the activity of beta-lactamase to some extent...
  46. pmc PBP 2a mutations producing very-high-level resistance to beta-lactams
    Yuki Katayama
    Division of Infectious Diseases, San Francisco General Hospital, University of California, San Francisco, California, USA
    Antimicrob Agents Chemother 48:453-9. 2004
    ..The emergence of PBP 2a-mediated resistance to beta-lactams that bind to PBP 2a with a high affinity is likely to require multiple mutations in mecA; chromosomal mutations appear to have a minor role...
  47. pmc Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci
    T Yamazumi
    Medical Microbiology Division, Department of Pathology University of Iowa College of Medicine, Iowa City, Iowa, USA
    J Clin Microbiol 39:3633-6. 2001
    ..Although the MRSA-Screen test required a slight procedural modification, both commercial methods achieved a sensitivity and specificity at detecting oxacillin resistance in CoNS at a level that was acceptable for clinical laboratory use...
  48. ncbi [Microbiology--laboratory examinations for bacterias]
    Renjun Hen
    Department of Clinical Laboratory Medicine, Fukushima Medical University, Fukushima 960 1247
    Rinsho Byori 50:1041-6. 2002
    ..The test was shown to be highly sensitive and specific. For the rapid identification of pathogenic microbes, simple and rapid test kits described here will be used more in clinical diagnosis...
  49. pmc Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis
    C Duez
    Centre d Ingénierie des Protéines and Laboratoire d Enzymologie, Institut de Chimie, Universite de Liege, B 4000 Liege, Belgium
    J Bacteriol 183:1595-9. 2001
    ..Cephaloridine, however, efficiently inactivates PBP4a (k(2)/K = 46,000 M(-1) s(-1)). PBP4a is also much more thermostable than the R39 enzyme...
  50. pmc RWJ-54428 (MC-02,479), a new cephalosporin with high affinity for penicillin-binding proteins, including PBP 2a, and stability to staphylococcal beta-lactamases
    Francois Malouin
    Essential Therapeutics, Inc, Mountain View, California 94043, USA
    Antimicrob Agents Chemother 47:658-64. 2003
    ..These data are consistent with its excellent activity against beta-lactam-resistant gram-positive bacteria...
  51. ncbi Bifunctional penicillin-binding proteins: focus on the glycosyltransferase domain and its specific inhibitor moenomycin
    Anne Marie Di Guilmi
    Institut de Biologie Structurale Jean Pierre Ebel CEA CNRS UMR 5075 UJF, Grenoble, France
    Curr Pharm Biotechnol 3:63-75. 2002
    ....
  52. pmc Recruitment of the mecA gene homologue of Staphylococcus sciuri into a resistance determinant and expression of the resistant phenotype in Staphylococcus aureus
    S W Wu
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 183:2417-24. 2001
    ..sciuri may be an evolutionary precursor of the methicillin resistance gene mecA of the pathogenic strains of MRSA...
  53. pmc The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration
    D L Popham
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
    J Bacteriol 177:4721-9. 1995
    ..The idea that loosely cross-linked, flexible cortex peptidoglycan has a mechanical activity involved in achieving spore core dehydration is not consistent with normal core dehydration in spores lacking only dacB...
  54. ncbi FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response
    L Wang
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    Mol Microbiol 29:731-40. 1998
    ..The expression of ftsK increased as part of the SOS response, and increased expression of ftsK conferred increased resistance to DNA damage...
  55. ncbi Identification of a new mutation in Escherichia coli that suppresses a pbpB (Ts) phenotype in the presence of penicillin-binding protein 1B
    F Garcia del Portillo
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain
    FEMS Microbiol Lett 68:7-13. 1991
    ..These results clearly give further support to the implication of PPB1B in the septation process in Escherichia coli...
  56. ncbi [Inflammatory response in pneumonia: are glucocorticosteroids useful?]
    C Agusti
    Arch Bronconeumol 39:143-5. 2003
  57. ncbi Resistance to beta-lactam antibiotics
    K Poole
    Department of Microbiology and Immunology, Queen s University, K7L 3N6, Kingston, Ontario, Canada
    Cell Mol Life Sci 61:2200-23. 2004
    ....
  58. pmc Penicillin-binding protein 1 of Staphylococcus aureus is essential for growth
    A Wada
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Tokyo 162, Japan
    J Bacteriol 180:2759-65. 1998
    ..Based on these observations, we concluded that pbpA is essential for the growth of methicillin-sensitive S. aureus...
  59. pmc Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications
    H F Chambers
    Medical Service, San Francisco General Hospital 94143, USA
    Clin Microbiol Rev 10:781-91. 1997
    ..Investigational agents that bind PBP 2a at low concentrations appear promising but have not been tested in humans. Alternatives to vancomycin are few due to the multiple drug resistances typical of methicillin-resistant staphylococci...
  60. ncbi Evaluation of bactericidal activity of cefotaxime and other beta-lactams by a novel method
    G Satta
    Istituto di Microbiologia dell Università di Siena
    Drugs 35:35-40. 1988
    ....
  61. pmc Lysis of Escherichia coli by beta-lactams which bind penicillin-binding proteins 1a and 1b: inhibition by heat shock proteins
    J K Powell
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202
    J Bacteriol 173:4021-6. 1991
    ..Since many environmental stresses promote the expression of HSPs, it is possible that their presence contributes an additional mechanism toward development in bacteria of phenotypic tolerance to beta-lactam antibiotics...
  62. pmc Point mutations in Staphylococcus aureus PBP 2 gene affect penicillin-binding kinetics and are associated with resistance
    C J Hackbarth
    Division of Infectious Diseases, San Francisco General Hospital, California 94110
    Antimicrob Agents Chemother 39:103-6. 1995
    ..These structural and biochemical changes may contribute to the strains' resistance to beta-lactam antibiotics...
  63. pmc Topographical and functional investigation of Escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis
    F Lefevre
    Institut de Pharmacologie et de Biologie Structurale, UPR 9062 du CNRS, Toulouse, France
    J Bacteriol 179:4761-7. 1997
    ..Finally, we have shown that the transglycosylase and the transpeptidase domains are separated by an inert linker region, where substitutions and insertions can be made without hindering the in vivo and in vitro activity of the protein...
  64. pmc Cloning, nucleotide sequence, mutagenesis, and mapping of the Bacillus subtilis pbpD gene, which codes for penicillin-binding protein 4
    D L Popham
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305
    J Bacteriol 176:7197-205. 1994
    ..A single transcription start site was detected just upstream of pbpD. The pbpD locus was mapped to the 275 to 280 degrees region of the chromosomal genetic map...
  65. ncbi Growth and division of Streptococcus pneumoniae: localization of the high molecular weight penicillin-binding proteins during the cell cycle
    Cecile Morlot
    Institut de Biologie Structurale J P Ebel CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Mol Microbiol 50:845-55. 2003
    ..pneumoniae...
  66. pmc Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus
    Y Katayama
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 45:1955-63. 2001
    ..haemolyticus clinical strains and that the IS431-mediated mecI gene deletion causes the expression of methicillin resistance through the derepression of mecA gene transcription...
  67. ncbi Complex relationship between acquisition of beta-lactam resistance and loss of virulence in Streptococcus pneumoniae
    V Rieux
    Institut National de la Sante et de la Recherche Medicale, Hopital Bichat Claude Bernard, Paris, France
    J Infect Dis 184:66-72. 2001
    ..2b and 23.2b.2x mutants had no change in MIC. Compensatory mutations are implicated in the revival of virulence...
  68. pmc Murein (peptidoglycan) binding property of the essential cell division protein FtsN from Escherichia coli
    Astrid Ursinus
    Universitat Tubingen, Fakultat fur Biologie, Lehrbereich Mikrobielle Genetik, Auf der Morgenstelle 28, 72076 Tubingen, Germany
    J Bacteriol 186:6728-37. 2004
    ..coli MC1061. We discuss the possibilities that the binding of FtsN to murein during cell division might either stabilize the septal region or might have a function unrelated to cell division...
  69. ncbi Analysis of the different molecular forms of penicillin-binding protein 1B in Escherichia coli ponB mutants lysogenized with specialized transducing lambda (ponB+) bacteriophages
    F Rojo
    Eur J Biochem 144:571-6. 1984
    ..coli chromosome and suggest that pbp 1b alpha is the first membrane-bound form of pbp 1b able to bind labelled beta-lactams, and is the precursor of pbp 1b beta which is, in turn, the precursor of pbp 1 beta gamma...
  70. pmc Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test
    M A Horstkotte
    , , D-20246 Hamburg, Germany
    J Clin Microbiol 39:3700-2. 2001
    ..Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 microg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population...
  71. pmc An amino acid substitution in penicillin-binding protein 3 creates pointed polar caps in Escherichia coli
    P E Taschner
    Department of Molecular Cell Biology, University of Amsterdam, The Netherlands
    J Bacteriol 170:4828-37. 1988
    ..The large change in the shape of the polar caps was accompanied by a small change in the kinetics of peptidoglycan synthesis and in the local rate of surface synthesis activity along the cell envelope...
  72. ncbi The basis for resistance to beta-lactam antibiotics by penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus
    Cosimo Fuda
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Biol Chem 279:40802-6. 2004
    ..The observed conformational changes are likely to be a hallmark for how this enzyme carries out its catalytic function in cross-linking the bacterial cell wall...
  73. ncbi Genomic analysis of penicillin-resistant Streptococcus pneumoniae in Southeastern Michigan
    Elizabeth K Yee
    Divisions of Pediatric Infectious Diseases, Department of Pediatrics University of Michigan, Ann Arbor, MI 48109 0244, USA
    J Infect 49:126-35. 2004
    ..The emergence of multidrug resistance within Streptococcus pneumoniae population was analysed, correlating penicillin resistance Pen(R) with secondary antibiotic resistance, capsular serotype, and genetic diversity among isolates...
  74. pmc Preparation and characterization of monoclonal antibodies against native membrane-bound penicillin-binding protein 1B of Escherichia coli
    T Den Blaauwen
    Department of Molecular Cell Biology, University of Amsterdam, The Netherlands
    J Bacteriol 171:1394-401. 1989
    ..This suggests that all the other monoclonal antibodies recognize conformational epitopes. These properties make the monoclonal antibodies suitable tools for further studies...
  75. ncbi FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division
    J C Chen
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Mol Microbiol 42:395-413. 2001
    ..coli cell division proteins assemble sequentially as a multimeric complex at the division site: first FtsZ, then FtsA and ZipA independently of each other, followed successively by FtsK, FtsQ, FtsL, FtsW, FtsI and FtsN...
  76. ncbi Modification of the peptidoglycan of Escherichia coli in the viable but nonculturable state
    Caterina Signoretto
    Dipartimento di Patologia, Sezione di Microbiologia, Universita di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Curr Microbiol 44:125-31. 2002
    ..Finally, VBNC cells displayed an autolytic capability which was far higher than that of exponentially growing cells. It is suggested that part of these alterations of peptidoglycan may be connected with the VBNC state...
  77. pmc Diversity of beta-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae
    Henri Dabernat
    Laboratoire de Microbiologie, Centre national de reference des Haemophilus influenzae, Toulouse, France
    Antimicrob Agents Chemother 46:2208-18. 2002
    ..The level of resistance remains low but opens the way to greater resistance in the future...
  78. ncbi [Clonal diffusion and evolution of mecA and Tn554 polymorphisms in methicillin-resistant Staphylococcus aureus in Italy]
    F Campanile
    Dipartimento di Scienze Microbiologiche e Scienze Ginecologiche Sezione di Microbiologia, Universit degli Studi di Catania
    Infez Med 9:30-8. 2001
    ....
  79. pmc A simple screen for murein transglycosylase inhibitors
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, 72076 Tubingen, Germany
    Antimicrob Agents Chemother 44:1181-5. 2000
    ..Crude membrane extracts can be used as a source for the PBPs, and different kinds of labels for the penicillin-PBP complexes can be used. The assay can be adapted to high-throughput screens...
  80. pmc Molecular characterization of multidrug-resistant Streptococcus pneumoniae isolates in Korea. The Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study Group
    J H Song
    Division of Infectious Diseases, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, Seoul, Korea
    J Clin Microbiol 38:1641-4. 2000
    ..8%) and amino acids (3.0 to 4. 3%), while Asn-276-->Lys, Arg-285-->Cys and Ser-305-->Phe substitutions were unique to Korean MDR strains, suggesting the spread of a few epidemic clones of resistant pneumococci within Korea...
  81. pmc Cloning, characterization, and inactivation of the gene pbpC, encoding penicillin-binding protein 3 of Staphylococcus aureus
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 182:1074-9. 2000
    ..Cells of abnormal size and shape and disoriented septa were produced when bacteria with inactivated pbpC were grown in the presence of a sub-MIC of methicillin...
  82. pmc Genotypes of invasive pneumococcal isolates recently recovered from Italian patients
    Giordano Dicuonzo
    Dipartimento di Medicina di Laboratorio e Microbiologia, Università Campus Bio Medico, Via Emilio Longoni 47, 00155 Rome, Italy
    J Clin Microbiol 40:3660-5. 2002
    ..One PSSP isolate was a novel type 24F isolate related to the multiresistant clone France(9V)-3. This work reports new PNSP strains and new serotype-clone associations...
  83. ncbi A kinetic characterization of the glycosyltransferase activity of Eschericia coli PBP1b and development of a continuous fluorescence assay
    Benjamin Schwartz
    Department of Chemical and Physical Sciences, Bristol Myers Squibb Company, Wilmington, Delaware 19880, USA
    Biochemistry 41:12552-61. 2002
    ....
  84. ncbi Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus
    H Murakami
    College of Medical Technology and Nursing, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan
    Biochem Biophys Res Commun 264:348-51. 1999
    ..Homology search revealed that Drp35 was a new protein. Our results revealed that it was specific in S. aureus and respondent to these agents in both methicillin-resistant and -sensitive strains of S. aureus...
  85. pmc Identification, purification, and characterization of transpeptidase and glycosyltransferase domains of Streptococcus pneumoniae penicillin-binding protein 1a
    A M Di Guilmi
    Institut de Biologie Structurale Jean Pierre Ebel CEA CNRS, 38027 Grenoble Cedex 1, France
    J Bacteriol 180:5652-9. 1998
    ..The GT domain (Ser 37 to Lys 263) was expressed as a recombinant GST fusion protein. Protection by moenomycin of the GT domain against trypsin degradation was interpreted as an interaction between the GT domain and the moenomycin...
  86. pmc Septal localization of penicillin-binding protein 1 in Bacillus subtilis
    L B Pedersen
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 181:3201-11. 1999
    ..subtilis. This is the first example of a high-molecular-weight class A PBP that is localized to the bacterial division septum...
  87. pmc Genetic organization of the mecA region in methicillin-susceptible and methicillin-resistant strains of Staphylococcus sciuri
    S Wu
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 180:236-42. 1998
    ..aureus...
  88. ncbi Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR
    Hakan Savli
    Tibbi Biyoloji AD, Kocaeli Universitesi, Tip Fakultesi, Sopali Ciftligi, 41900 Kocaeli, Turkey
    J Med Microbiol 52:403-8. 2003
    ....
  89. ncbi [Cephem antibiotics]
    Hajime Goto
    First Department of Internal Medicine, Kyorin University School of Medicine
    Nihon Rinsho 61:731-6. 2003
  90. pmc Penicillin-binding protein 5 and expression of ampicillin resistance in Enterococcus faecium
    L B Rice
    Medical and Research Services, VA Medical Center, Cleveland, Ohio 44106, USA
    Antimicrob Agents Chemother 45:1480-6. 2001
    ..However, quantities of psr transcript were increased in D344-SRF(pCWR583) compared to D344-SRF(pCWR558), especially after growth in ampicillin; suggesting that PSR acts in some manner to activate its own transcription...
  91. pmc Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression
    D Vinella
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universite Paris 7, France
    J Bacteriol 175:6704-10. 1993
    ..The plasmid pAQ, in which the ftsZ gene is inactivated, did not confer amdinocillin resistance. These results lead us to hypothesize that the nucleotide ppGpp activates ftsZ expression and thus couples cell division to protein synthesis...
  92. ncbi Analysis of Staphylococcus aureus genes encoding penicillin-binding protein 4 and an ABC-type transporter
    T L Domanski
    Department of Biological Sciences, University of Maryland, Baltimore 21228, USA
    Gene 167:111-3. 1995
    ..Based on these observations, a potential regulatory link between these two genes is discussed...
  93. ncbi X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzyme
    S Pares
    Institut de Biologie Structurale, Laboratoire de Cristallographie Macromoléculaire, Grenoble, France
    Nat Struct Biol 3:284-9. 1996
    ..5 A resolution. The molecule has three domains, the central domain being a transpeptidase, which is a suitable target for antibiotic development...
  94. pmc Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression
    X Liao
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    J Bacteriol 179:1490-6. 1997
    ..3H]penicillin-binding assays indicated that PBP3 was mainly produced during exponential growth whereas PBP3x was produced in the stationary phase of growth...
  95. ncbi Overproduction of penicillin-binding protein 7 suppresses thermosensitive growth defect at low osmolarity due to an spr mutation of Escherichia coli
    H Hara
    National Institute of Genetics, Shizuoka ken, Japan
    Microb Drug Resist 2:63-72. 1996
    ..Overproduction of mutant PBP 7 that had the active site serine residue replaced with alanine did not correct the spr thermosensitivity, suggesting importance of the DD-endopeptidase activity in the multicopy suppression...
  96. ncbi Penicillin-binding protein 5 as an inhibitory target of cefozopran in Enterococcus faecalis
    N Tsuchimori
    Takeda Chemical Industries Ltd, Osaka 532, Japan
    J Antimicrob Chemother 37:605-9. 1996
    ..No correlation between MICs of the test compounds and the affinity for PBP 1, 2 or 6 was found. These results suggest that cefozopran exhibits antimicrobial activity against E. faecalis TN2OO5 by binding to PBP 5...
  97. pmc Affinities of beta-lactams for penicillin binding proteins of Chlamydia trachomatis and their antichlamydial activities
    C Storey
    Division of Microbiology and Antimicrobial Research Centre, University of Leeds, Leeds LS2 9JT, United Kingdom
    Antimicrob Agents Chemother 45:303-5. 2001
    ..1 microg/ml) and minimum bactericidal concentration (0.25 microg/ml). Although the other beta-lactams inhibited a wider range of PBPs than mecillinam, their antichlamydial activities were inferior to that of mecillinam...
  98. pmc Sensitivity of amoxicillin-resistant Helicobacter pylori to other penicillins
    M P Dore
    Department of Medicine, VA Medical Center, and Baylor College of Medicine, Houston, Texas 77030, USA
    Antimicrob Agents Chemother 43:1803-4. 1999
    ..High-level resistance was associated with the smallest zone size for all penicillins tested...
  99. ncbi Peptidoglycan assembly machines: the biochemical evidence
    André Zapun
    Commissariat à l Energie Nucléaire, Institut de Biologie Structurale, Grenoble, France
    Microb Drug Resist 18:256-60. 2012
    ..We review here the reports of isolation of complexes comprising penicillin-binding proteins (PBPs), the enzymes that synthesize the peptidoglycan from its lipid-linked precursor...
  100. pmc Identification of Treponema pallidum penicillin-binding proteins
    T M Cunningham
    Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90024
    J Bacteriol 169:5298-300. 1987
    ..Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plasma membrane intactness in T. pallidum fractionation studies...
  101. pmc Staphylococcus aureus penicillin-binding protein 4 and intrinsic beta-lactam resistance
    U U Henze
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Antimicrob Agents Chemother 39:2415-22. 1995
    ..The abcA-pbp4-tagD gene cluster was located in the SmaI-D fragment in the S. aureus 8325 chromosome in close proximity to the RNA polymerase gene rpoB...

Research Grants10

  1. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
    ..abstract_text> ..
  2. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
    ....
  3. Acquisition of a 600 MHz NMR Spectrometer - Wayne State
    Shahriar Mobashery; Fiscal Year: 2002
    ..The availability of this instrument would stimulate further research in structural biology at Wayne State University, enhancing the strong existing capabilities that we enjoy in X-ray structure determination. ..
  4. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..Together, these projects utilize genetic, biochemical, biophysical, and proteomics approaches to generate new insights into the mechanisms of antibiotic action in N. gonorrhoeae. ..
  5. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2010
    ..The proposed work is to study how resistance to beta-lactam antibiotics has developed. The understanding of the mechanistic details should guide the way in devising strategies to circumvent the problem. ..
  6. RIBOSOMAL FUNCTION & ANTIBIOTIC DESIGN
    Shahriar Mobashery; Fiscal Year: 2007
    ..This collective effort will shed light both on the various molecular events within the ribosomal and on how to inhibit some of these processes. [unreadable] [unreadable]..
  7. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..
  8. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
    ..Our studies will provide novel and important information on the cellular mechanisms of P2Y receptor targeting and the regulation of P2Y receptor signaling activity in epithelial cells. ..
  9. ASSEMBLY OF THE DIVISION SEPTUM IN ESCHERICHIA COLI
    David Weiss; Fiscal Year: 2003
    ..In this regard, it is worth noting that four of the proteins to be studied here (FtsI and the three transglycosylases) are primary targets of beta-lactam antibiotics. ..
  10. PENICILLIN INTERACTIVE PROTEINS OF STAPHYLOCOCCUS AUREUS
    Henry Chambers; Fiscal Year: 2003
    ..To augment information about where PBPs localize, when they are expressed during the cell cycle will be determined by Northern blotting. ..