enteropeptidase

Summary

Summary: A specialized proteolytic enzyme secreted by intestinal cells. It converts TRYPSINOGEN into its active form TRYPSIN by removing the N-terminal peptide. EC 3.4.21.9.

Top Publications

  1. pmc Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
    Kevin T Boulware
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 103:7583-8. 2006
  2. ncbi Trypsin IV, a novel agonist of protease-activated receptors 2 and 4
    Graeme S Cottrell
    Departments of Surgery and Physiology, University of California San Francisco, San Francisco, California 94143 0660, USA
    J Biol Chem 279:13532-9. 2004
  3. ncbi Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield
    Peter Simeonov
    Ultrasensitive Protein Detection Unit, Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany
    Protein Eng Des Sel 24:261-8. 2011
  4. ncbi Keratinocytes synthesize enteropeptidase and multiple forms of trypsinogen during terminal differentiation
    Jotaro Nakanishi
    Shiseido Research Center, Yokohama, Japan
    J Invest Dermatol 130:944-52. 2010
  5. ncbi Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli
    Liu di Yuan
    Department of Biochemistry, State Key Laboratory of Pharmaceutical Biotechnology and Institute of Molecular and Cell Biology, College of Life Sciences, Nanjing University, Nanjing 210093, People s Republic of China
    Protein Expr Purif 25:300-4. 2002
  6. ncbi Substrate specificities of porcine and bovine enteropeptidases toward the peptide Val-(Asp)4-Lys-Ile-Val-Gly and its analogs
    Yong Tae Kim
    Protein Research Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    Biosci Biotechnol Biochem 72:905-8. 2008
  7. ncbi Activation of recombinant proenteropeptidase by duodenase
    T S Zamolodchikova
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    FEBS Lett 466:295-9. 2000
  8. ncbi Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein
    T Hosfield
    Stratagene Cloning Systems, Inc, La Jolla, California 92037, USA
    Anal Biochem 269:10-6. 1999
  9. ncbi Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris
    L A Vozza
    Invitrogen Corporation, San Diego, CA 92121, USA
    Biotechnology (N Y) 14:77-81. 1996
  10. ncbi Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli
    Haidong Tan
    Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China
    Protein Expr Purif 56:40-7. 2007

Research Grants

  1. Fluorogenic Assays for Factor Vlla and Tissue Factor
    RICHARD JENNY; Fiscal Year: 2006
  2. ENTEROKINASE--AN INTESTINAL DIGESTIVE PROTEASE
    J Sadler; Fiscal Year: 2003
  3. Mechanism and Therapies for HAART Induced Diarrhea
    Tibor Sipos; Fiscal Year: 2001
  4. STRUCTURE/FUNCTION OF SURFACTANT PROTEIN D
    Erika Crouch; Fiscal Year: 2006
  5. Bionanotechnology for Breast and Prostate Cancer
    Elizabeth Singer; Fiscal Year: 2007
  6. A Global Test of Hemostatic Risk
    RICHARD JENNY; Fiscal Year: 2008
  7. Structure and Function of ADAMTS13 Metalloprotease
    X Long Zheng; Fiscal Year: 2009

Detail Information

Publications203 found, 100 shown here

  1. pmc Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
    Kevin T Boulware
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 103:7583-8. 2006
    ..of CLiPS was demonstrated by determining the substrate specificity of two unrelated proteases, caspase-3 and enteropeptidase (or enterokinase). CLiPS unambiguously identified the caspase-3 consensus cleavage sequence DXVDG...
  2. ncbi Trypsin IV, a novel agonist of protease-activated receptors 2 and 4
    Graeme S Cottrell
    Departments of Surgery and Physiology, University of California San Francisco, San Francisco, California 94143 0660, USA
    J Biol Chem 279:13532-9. 2004
    ..from prostate, colon, and airway and human colonic mucosa expressed mRNA encoding PAR(2), trypsinogen IV, and enteropeptidase, which activates the zymogen. Immunoreactive trypsinogen IV was detected in vesicles in these cells...
  3. ncbi Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield
    Peter Simeonov
    Ultrasensitive Protein Detection Unit, Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany
    Protein Eng Des Sel 24:261-8. 2011
    b>Enteropeptidase is a serine protease used in different biotechnological applications. For many applications the smaller light chain can be used to avoid the expression of the rather large holoenzyme...
  4. ncbi Keratinocytes synthesize enteropeptidase and multiple forms of trypsinogen during terminal differentiation
    Jotaro Nakanishi
    Shiseido Research Center, Yokohama, Japan
    J Invest Dermatol 130:944-52. 2010
    ..Both isoforms contained the sequence DDDDK-I, a putative cleavage site for activation by enteropeptidase. Thus, after activation, mesotrypsin would be produced...
  5. ncbi Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli
    Liu di Yuan
    Department of Biochemistry, State Key Laboratory of Pharmaceutical Biotechnology and Institute of Molecular and Cell Biology, College of Life Sciences, Nanjing University, Nanjing 210093, People s Republic of China
    Protein Expr Purif 25:300-4. 2002
    ..The purified intact EK catalytic subunit has a K(m) of 0.17 mM, and K(cat) is 20.8s(-1). From 100 ml flask culture, 4.3 mg pure active EK catalytic subunits were obtained...
  6. ncbi Substrate specificities of porcine and bovine enteropeptidases toward the peptide Val-(Asp)4-Lys-Ile-Val-Gly and its analogs
    Yong Tae Kim
    Protein Research Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    Biosci Biotechnol Biochem 72:905-8. 2008
    ..These results were essentially identical as between porcine and bovine enteropeptidases...
  7. ncbi Activation of recombinant proenteropeptidase by duodenase
    T S Zamolodchikova
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    FEBS Lett 466:295-9. 2000
    ..a serine proteinase from bovine Brunner's (duodenal) glands that was predicted to be a natural activator of enteropeptidase zymogen, cleaves and activates recombinant single-chain bovine proenteropeptidase (kcat/Km = 2700 M(-1) s(-1))..
  8. ncbi Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein
    T Hosfield
    Stratagene Cloning Systems, Inc, La Jolla, California 92037, USA
    Anal Biochem 269:10-6. 1999
    ..This makes EK an ideal choice to use as a protease to cleave any protein of interest cloned within the pESP4 yeast expression vector...
  9. ncbi Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris
    L A Vozza
    Invitrogen Corporation, San Diego, CA 92121, USA
    Biotechnology (N Y) 14:77-81. 1996
    ..Its application is demonstrated by the removal of thioredoxin (TrxA), and polyhistidine fusion partners from proteins of interest...
  10. ncbi Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli
    Haidong Tan
    Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China
    Protein Expr Purif 56:40-7. 2007
    ..No. E4906). This procedure produced approximately 53 mg of EK per 500 mL of cell culture, which was much higher than previous reports, thus providing a basis for large-scale production of EK and for further applications in biotechnology...
  11. ncbi Crystal structure of a supercharged variant of the human enteropeptidase light chain
    Peter Simeonov
    Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Universitat Leipzig, 04103 Leipzig, Germany
    Proteins 80:1907-10. 2012
    The highly specific serine protease human enteropeptidase light chain cleaves the Asp4Lys recognition sequence and represents an interesting enzyme for biotechnological applications...
  12. ncbi Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli
    Haarin Chun
    Department of Bioinformatics and Life Science, Soongsil University, Seoul, 156 743, Korea
    Biotechnol Lett 33:1227-32. 2011
    ..The production of EKL with higher specificity could widen the utility of EKL as a site-specific cleavage enzyme to produce various recombinant proteins with therapeutic or industrial values...
  13. ncbi Strategy for improvement of enteropeptidase efficiency in tag removal processes
    Marine E Gasparian
    Department of Bioengineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, 16 10 Miklukho Maklaya, 117997 Moscow, Russia
    Protein Expr Purif 79:191-6. 2011
    b>Enteropeptidase (synonym: enterokinase, EC 3.4.21...
  14. ncbi High level expression of human enteropeptidase light chain in Pichia pastoris
    Stanislav Pepeliaev
    CPN spol s r o, Dolni Dobrouc 401, 56102 Dolní Dobrouč, Czech Republic
    J Biotechnol 156:67-75. 2011
    Human enterokinase (enteropeptidase, rhEP), a serine protease expressed in the proximal part of the small intestine, converts the inactive form of trypsinogen to active trypsin by endoproteolytic cleavage...
  15. ncbi Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification
    Hye Won Song
    Protheon Incorporated, Yonsei Engineering Center B120E, Seoul 120 749, Korea
    Arch Biochem Biophys 400:1-6. 2002
    b>Enteropeptidase (enterokinase) is a serine protease highly specific for recognition and cleavage of the target sequence of Asp-Asp-Asp-Asp-Lys (D4K)...
  16. ncbi Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum
    Takahisa Imamura
    Division of Molecular Pathology, Kumamoto University Graduate School of Medical and Pharmaceutical Sciences, Kumamoto 860 0811, Japan
    Am J Physiol Gastrointest Liver Physiol 285:G1235-41. 2003
    b>Enteropeptidase (EP) is a serine proteinase and activates trypsinogen to trypsin, thus playing an important role in food digestion...
  17. ncbi Enteropeptidase, a type II transmembrane serine protease
    X Long Zheng
    Department of Pathology and Laboratory Medicine, The Children s Hospital of Philadelphia and the University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Front Biosci (Elite Ed) 1:242-9. 2009
    b>Enteropeptidase, a type II transmembrane serine protease, is localized to the brush border of the duodenal and jejunal mucosa...
  18. ncbi Enzymatic properties of rat myelencephalon-specific protease
    Sachiko I Blaber
    Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306 4380, USA
    Biochemistry 41:1165-73. 2002
    ....
  19. ncbi Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase
    E R Lavallie
    Genetics Institute, Inc, Cambridge, Massachusetts 02140
    J Biol Chem 268:23311-7. 1993
    Enterokinase (enteropeptidase) is a heterodimeric serine protease that is responsible for the physiological activation of trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)4-Lys...
  20. ncbi Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli
    Marine E Gasparian
    Laboratory of Protein Engineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, 16 10 Miklukho Maklaya, 117997 GSP, Moscow, Russia
    Protein Expr Purif 31:133-9. 2003
    b>Enteropeptidase (synonym:enterokinase, EC 3.4.21...
  21. ncbi A proteolytic method for distinguishing between lipid-free and lipid-bound apolipoprotein A-I
    W Safi
    Department of Pathology and Laboratory Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, OH 45267-0529, USA
    J Lipid Res 42:864-72. 2001
    ..As part of a search for a rapid assay for these forms of apoA-I, we have observed that the protease enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form...
  22. ncbi Intracellular co-localization of trypsin-2 and matrix metalloprotease-9: possible proteolytic cascade of trypsin-2, MMP-9 and enterokinase in carcinoma
    Suvi Tuuli Vilen
    Institute of Dentistry, University of Helsinki, Department of Oral and Maxillofacial Diseases, Helsinki University Hospital, Finland
    Exp Cell Res 314:914-26. 2008
    ....
  23. ncbi Biochemical characterization of human enteropeptidase light chain
    M E Gasparian
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
    Biochemistry (Mosc) 71:113-9. 2006
    The synthetic gene encoding human enteropeptidase light chain (L-HEP) was cloned into plasmid pET-32a downstream from the gene of fusion partner thioredoxin immediately after the DNA sequence encoding the enteropeptidase recognition site...
  24. ncbi Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development
    X Yuan
    Howard Hughes Medical Institute, Department of Medicine, Barnes Jewish Hospital, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Physiol 274:G342-9. 1998
    Enterokinase (enteropeptidase) is expressed only in proximal small intestine, where it initiates digestive enzyme activation by converting trypsinogen into trypsin...
  25. ncbi Overexpression and refolding of thioredoxin/TRAIL fusion from inclusion bodies and further purification of TRAIL after cleavage by enteropeptidase
    Marine E Gasparian
    Laboratory of Protein Engineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, 16 10 Miklukho Maklaya, Moscow, 117997 GSP, Russia
    Biotechnol Lett 29:1567-73. 2007
    ..Refolded fusion thioredoxin/TRAIL was cleaved by enteropeptidase and TRAIL was separated from thioredoxin on Ni-NTA agarose...
  26. pmc Mutations in the proenteropeptidase gene are the molecular cause of congenital enteropeptidase deficiency
    Andreas Holzinger
    Department of Pediatrics, Division of Clinical Chemistry and Metabolism, Dr V Hauner Children s Hospital, Ludwig Maximilian University, Munich, Germany
    Am J Hum Genet 70:20-5. 2002
    b>Enteropeptidase (enterokinase [E.C.3.4.21.9]) is a serine protease of the intestinal brush border in the proximal small intestine...
  27. ncbi Structural characterization of porcine enteropeptidase
    M Matsushima
    First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan
    J Biol Chem 269:19976-82. 1994
    b>Enteropeptidase (EC 3.4.21.9) is a key enzyme in the intestinal digestion cascade responsible for the conversion of trypsinogen to trypsin, which then activates various pancreatic zymogens...
  28. pmc The tetra-aspartate motif in the activation peptide of human cationic trypsinogen is essential for autoactivation control but not for enteropeptidase recognition
    Zsofia Nemoda
    Department of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Massachusetts 02118, USA
    J Biol Chem 280:29645-52. 2005
    ..A large body of research has defined the primary role of this acidic motif as a specific recognition site for enteropeptidase, the physiological activator of trypsinogen...
  29. ncbi The MUC1 SEA module is a self-cleaving domain
    Fiana Levitin
    Department of Cell Research and Immunology, Tel Aviv University, Ramat Aviv 69978, Israel
    J Biol Chem 280:33374-86. 2005
    ..It is likely that all cleaved SEA module-containing proteins follow a similar route...
  30. ncbi Enterokinase cleavage of fusion proteins for preparation of recombinant human parathyroid hormone 1-34
    Zhao Yang Xiu
    Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Science, Shanghai 200233, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:469-74. 2002
    ....
  31. ncbi Expression of a cold-adapted fish trypsin in Pichia pastoris
    Martin Macouzet
    Department of Food Science and Agricultural Chemistry, McGill University, Macdonald Campus, Ste Anne de Bellevue, QC, Canada
    FEMS Yeast Res 5:851-7. 2005
    ..Immobilized nickel affinity chromatography yielded a partially purified recombinant CFT, which exhibited trypsin-specific activity after activation with bovine enterokinase...
  32. ncbi Expression and preparation of recombinant hepcidin in Escherichia coli
    Huai Zhang
    Department of Pharmaceutical Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China
    Protein Expr Purif 41:409-16. 2005
    ..Following monomer refolding, hepcidin was cleaved from fusion protein by enterokinase and purified with reverse-phase chromatography. The recombinant hepcidin exhibited obvious antibacterial activity against Bacillus subtilis...
  33. ncbi Effect of calcium ions on enteropeptidase catalysis
    A G Mikhailova
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia
    Biochemistry (Mosc) 70:1129-35. 2005
    The effects of calcium ions on hydrolysis of low molecular weight substrates catalyzed by different forms of enteropeptidase were studied...
  34. ncbi Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites
    Oi Wah Liew
    Deputy Principal Academic s Office, Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore
    Protein Expr Purif 41:332-40. 2005
    ..Our results showed that non-target sequences can be preferentially recognized over the canonical DDDDK sequence when located accessibly at the ends of proteins...
  35. ncbi Large scale production of recombinant antibodies by utilizing cellulose-binding domains
    Itai Benhar
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
    Methods Mol Biol 207:443-54. 2003
  36. ncbi One-step purification of chimeric green fluorescent protein providing metal-binding avidity and protease recognition sequence
    Virapong Prachayasittikul
    Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand
    Asian Pac J Allergy Immunol 21:259-67. 2003
    ..This concludes that engineering of repetitive histidine regions onto interested target protein along with the enterokinase cleavage sites will ease the complication of protein purification...
  37. ncbi Functional analysis of the transmembrane domain and activation cleavage of human corin: design and characterization of a soluble corin
    Sabine Knappe
    Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA
    J Biol Chem 278:52363-70. 2003
    ....
  38. ncbi Expression of recombinant chinese bovine enterokinase catalytic subunit in P. pastoris and its purification and characterization
    Lei Fang
    The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
    Acta Biochim Biophys Sin (Shanghai) 36:513-7. 2004
    ..4 mg per liter of fermentation culture. This purified EKL-His6 demonstrates excellent cleavage activity towards fusion protein containing EK cleavage site...
  39. ncbi Inhibition of membrane-type serine protease 1/matriptase by natural and synthetic protease inhibitors
    Yoshie Yamasaki
    Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Nutr Sci Vitaminol (Tokyo) 49:27-32. 2003
    ..The present findings provide important information for the suppression of cancer invasion and metastasis for which MT-SP1/matriptase is responsible...
  40. ncbi Large scale preparation of recombinant human parathyroid hormone 1-84 from Escherichia coli
    Qinghai Liu
    State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, PR China
    Protein Expr Purif 54:212-9. 2007
    ..The efficient procedure for expression and purification of rhPTH(1-84) may be useful for the mass production of this important protein...
  41. ncbi [New substrates for enteropeptidase. I. Biologically active hepta-nonapeptides]
    V V Likhareva
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, GSP Moscow, 117997 Russia
    Bioorg Khim 29:129-34. 2003
    b>Enteropeptidase (enterokinase, EC 3.4.21.9) hydrolyzes peptide bonds formed by carboxyl groups of Lys or Arg residue provided that less than four negatively charged amino acid residues are in positions P2-P5 of its substrate...
  42. ncbi [Inhibition by IFN-(Asp)4-Lys-HIV chimeric protein of hydrolysis of the low molecular substrate by the enteropeptidase light chain]
    E D Shibanova
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, GSP 7, Moscow, 117997 Russia
    Vopr Med Khim 48:599-602. 2002
    The full length enteropeptidase or it's light chain have often used for the limited proteolysis of recombinant chimeric proteins incorporating the linker-(Asp)4Lys- to obtain the target protein...
  43. ncbi Ligand specificity of human surfactant protein D: expression of a mutant trimeric collectin that shows enhanced interactions with influenza A virus
    Erika Crouch
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:17046-56. 2005
    ..In addition, RAK resembled CL-43 in its greater capacity to inhibit the infectivity of influenza A virus and to increase uptake of influenza by neutrophils...
  44. ncbi Study of secondary specificity of enteropeptidase in comparison with trypsin
    A G Mikhailova
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia
    Biochemistry (Mosc) 69:909-17. 2004
    A comparative study of secondary specificities of enteropeptidase and trypsin was performed using peptide substrates with general formula A-(Asp/Glu)n-Lys(Arg)-(downward arrow)-B, where n = 1-4...
  45. ncbi Cloning and expression of ostrich trypsinogen: an avian trypsin with a highly sensitive autolysis site
    Borbála Szenthe
    Department of Biochemistry, Eotvos Lorand University, Pazmany Peter setany 1 C, H 1117 Budapest, Hungary
    Biochim Biophys Acta 1748:35-42. 2005
    ..These appear to be hydrolysed fast in a non-consecutive manner. Such an autolysis mechanism could not be inhibited by a single-site mutation which in humans is proposed to lead to pancreatitis...
  46. ncbi Prokaryotic expression of Chinese bovine enterokinase catalytic subunit
    He Huang
    Department of Biochemical Engineering, School of Chemical Engineering, Tianjin University, China
    Chin Med J (Engl) 117:286-90. 2004
    ..To express in vitro the bovine enterokinase catalytic subunit (EKL) protein, which could be used in the future for the cleavage and purification of fusion proteins...
  47. ncbi High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris
    Lisheng Peng
    The Open Laboratory for Marine Functional Genomics of State High Tech Development, Department of Biochemistry, College of Life Sciences, Sun Yat Sen Zhongshan University, Guangzhou 510275, PR China
    J Biotechnol 108:185-92. 2004
    ..pastoris, and this His-tagged EK(L) exhibited a similar enzymatic activity to the untagged EK(L)...
  48. ncbi Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology
    S I Choi
    Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, Seoul 120-749, Korea
    Biotechnol Bioeng 75:718-24. 2001
    ..The simple affinity tag renders rEK(L) extremely useful for purification, post-cleavage removal, recovery, and recycling and will broaden the utility and the versatility of the enterokinase for the production of recombinant proteins...
  49. ncbi Purification and characterization of RGD tumor-homing peptide conjugated human tumor necrosis factor alpha over-expressed in Escherichia coli
    Dingyuan Ma
    The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, PR China
    J Chromatogr B Analyt Technol Biomed Life Sci 857:231-9. 2007
    ..Since the RGD-hTNF molecule retained the cytotoxic activity of the TNF moiety and the integrin binding ability of the RGD moiety, the purification method provided material for assessing its anti-tumor activity in animal model...
  50. ncbi Expression and characterization of the carboxyl esterase Rv3487c from Mycobacterium tuberculosis
    Min Zhang
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China
    Protein Expr Purif 42:59-66. 2005
    ..The catalytic triad of Rv3487c consists of residues Ser90, Glu189, and His219 as demonstrated by amino acid sequence alignment, three-dimensional modeling, and site-directed mutagenesis...
  51. ncbi Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis
    Joseph D Platko
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
    Protein Expr Purif 57:57-62. 2008
    ..These data demonstrate the successful expression of Mcfp-3 in K. lactis and show an intrinsic ability of Mcfp-3 to self-assemble into stable, higher molecular weight forms...
  52. ncbi Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage
    Tina Kubitzki
    Institute of Biotechnology 2, Research Centre Juelich GmbH, 52425 Julich, Germany
    Bioprocess Biosyst Eng 31:173-82. 2008
    ..The immobilised enterokinase cleaves the fusion protein MUC1-IgG Fc in at least two repeated batches, proving the efficiency of the immobilisation method and the reusability of the biocatalyst...
  53. ncbi Interactions of pancreatic secretory trypsin inhibitor in small intestinal juice: its hydrolysis and protection by intraluminal factors
    T C Freeman
    Department of Medicine, Royal Postgraduate Medical School, London, UK
    Clin Chim Acta 195:27-39. 1990
    ..Our findings support the hypothesis that PSTIs mediate the modulation of CCK release by intraluminal proteinases, proteinase inhibitors and proteins...
  54. ncbi Induction of a selective and persistent extravasation of neutrophils into the peritoneal cavity by tryptase mouse mast cell protease 6
    C Huang
    Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Immunol 160:1910-9. 1998
    ....
  55. pmc Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains
    Y Kitamoto
    Department of Medicine, Jewish Hospital of St Louis, Washington University School of Medicine, MO 63110
    Proc Natl Acad Sci U S A 91:7588-92. 1994
    ..The specificity of enterokinase for the DDDDK-I sequence of trypsinogen may be explained by complementary basic-amino acid residues clustered in potential S2-S5 subsites...
  56. ncbi cDNA sequence and chromosomal localization of human enterokinase, the proteolytic activator of trypsinogen
    Y Kitamoto
    Howard Hughes Medical Institute, Department of Surgery, Jewish Hospital of St Louis, Washington University School of Medicine, Missouri 63110, USA
    Biochemistry 34:4562-8. 1995
    ..By Northern blotting, a 4.4 kb enterokinase mRNA was detected only in small intestine. The enterokinase gene was localized to human chromosome 21q21 by fluorescence in situ hybridization...
  57. ncbi Mesotrypsin: a new inhibitor-resistant protease from a zymogen in human pancreatic tissue and fluid
    H Rinderknecht
    Gastroenterology 86:681-92. 1984
    ..It is activated by enteropeptidase or trypsin, and the free enzyme possesses a substrate specificity similar to that of the trypsins...
  58. ncbi Trypsin IV or mesotrypsin and p23 cleave protease-activated receptors 1 and 2 to induce inflammation and hyperalgesia
    Wolfgang Knecht
    Molecular Pharmacology and Lead Generation, AstraZeneca Research and Development, Mölndal 431 83, Sweden
    J Biol Chem 282:26089-100. 2007
    ..b>Enteropeptidase cleaved both zymogens, liberating active trypsin IV and p23, which were resistant to soybean trypsin ..
  59. ncbi The acidity of protein fusion partners predominantly determines the efficacy to improve the solubility of the target proteins expressed in Escherichia coli
    Yu Su
    School of Life Sciences, East China Normal University, Zhongshan North Road 3663, Shanghai 200062, China
    J Biotechnol 129:373-82. 2007
    ..Meanwhile, the roles of other parameters such as protein hydrophilicity in solubility enhancement were discussed, a guideline to design or search an optimum protein solubility enhancer was also proposed...
  60. ncbi An SRLLR motif downstream of the scissile bond enhances enterokinase cleavage efficiency
    Oi Wah Liew
    Deputy Principal Academic s Office, Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore
    Biochimie 89:21-9. 2007
    ....
  61. ncbi The ways of realization of high specificity and efficiency of enteropeptidase
    Anna G Mikhailova
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russian Federation
    Protein Pept Lett 14:227-32. 2007
    Comparative substrate analysis of full-length bovine enteropeptidase and trypsin, bovine and human enteropeptidase light chains was performed using model N-terminal dodecapeptides corresponding to wild-type human trypsinogen and ..
  62. ncbi Tyrosine-based "activatable pro-tag": enzyme-catalyzed protein capture and release
    Angela T Lewandowski
    Center for Biosystems Research, University of Maryland Biotechnology Institute, 5115 Plant Sciences Building, College Park, Maryland 20742, USA
    Biotechnol Bioeng 93:1207-15. 2006
    ..Using GFP as a model protein, we demonstrated that enzymatic capture and release provides a simple, non-chromatographic means to recover proteins directly from cell lysates...
  63. ncbi Compatible solutes as protectants for zymogens against proteolysis
    Sonja Kolp
    Pharmaceutical Institute, Poppelsdorf, University of Bonn, Kreuzbergweg 26, D 53115 Bonn, Germany
    Biochim Biophys Acta 1764:1234-42. 2006
    ..hydroxyectoine and betaine on the activation of the zymogens trypsinogen and chymotrypsinogen, catalyzed by enteropeptidase and trypsin, respectively, was studied...
  64. ncbi Production of polyclonal antibodies against the human respiratory syncytial virus nucleoprotein and phosphoprotein expressed in Escherichia coli
    Fernando M Simabuco
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Avenida Professor Lineu Prestes, 1374, São Paulo SP 05508 900, Brazil
    Protein Expr Purif 53:209-15. 2007
    ..We now intend to purify the cleaved N and P proteins and use them in structural studies. The recombinant proteins will also be tested as potential inducers of a protective immunity against the HRSV...
  65. ncbi Expression of deletion mutants of the hepatitis B virus protein HBx in E. coli and characterization of their RNA binding activities
    E Rui
    Centro de Biologia Molecular Estrutural (CBME, , , Campinas-SP, Brazil
    Virus Res 74:59-73. 2001
    ..This new aspect of HBx function is discussed in the context of cellular transformation...
  66. ncbi Lectin-based affinity tag for one-step protein purification
    Denis Tielker
    Heinrich Heine University Duesseldorf, Juelich, Germany
    Biotechniques 41:327-32. 2006
    ..In conclusion, our results indicate that the lectin LecB of P. aeruginosa can be used as a tag for the high-yield one-step purification of recombinant proteins...
  67. ncbi A cyclophilin-like peptidyl-prolyl cis/trans isomerase from Legionella pneumophila--characterization, molecular cloning and overexpression
    B Schmidt
    Max Planck Gesellschaft, Arbeitsgruppe Enzymologie der Peptidbindung, Halle Saale, Germany
    Mol Microbiol 21:1147-60. 1996
    ..Like human cyclophilin, the L. p. Cyp18 exhibits nuclease activity, but this enzymatic activity does not appear to be linked with the native structure of the protein...
  68. ncbi Horse cytokine/IgG fusion proteins--mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines
    Bettina Wagner
    Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Hungerford Hill Road, Ithaca, NY 14853, USA
    Vet Immunol Immunopathol 105:1-14. 2005
    ....
  69. ncbi Cathepsin B activates human trypsinogen 1 but not proelastase 2 or procarboxypeptidase B
    Bjorn Lindkvist
    Department of Surgery, Malmo University Hospital, Lund University, Malmo, Sweden
    Pancreatology 6:224-31. 2006
    ..The aim of this study was to investigate cathepsin B and tryptase activation of pancreatic zymogens...
  70. ncbi The effect of endothelin-1, endothelin-2 and endothelin-3 in early cerulein-induced acute pancreatitis in rats
    J W Długosz
    Department of Gastroenterology and Internal Medicine, Medical University of Białystok, Poland
    Rocz Akad Med Bialymst 49:85-92. 2004
    ..To assess the effect of endothelins: ET-1, ET-2 and ET-3 on trypsinogen activation, lipase activity and histological changes in the pancreas in early (4 hrs) cerulein acute pancreatitis (AP) in rats...
  71. ncbi Role of various phospholipases A2 and inhibitors in the pathogenesis and prevention of pancreatic acinar cell necrosis: studies with isolated rat pancreatic acini
    J MOssner
    Department of Internal Medicine II, University of Leipzig, Germany
    Int J Pancreatol 27:29-38. 2000
    ....
  72. pmc Identification by heterologous expression and gene disruption of VisA as L-lysine 2-aminotransferase essential for virginiamycin S biosynthesis in Streptomyces virginiae
    Wises Namwat
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 184:4811-8. 2002
    ....
  73. pmc Effect of endothelin-1 receptor antagonists on histological and ultrastructural changes in the pancreas and trypsinogen activation in the early course of caerulein-induced acute pancreatitis in rats
    Anna Andrzejewska
    Department of Medical Pathomorphology, Medical University of Bialystok, Waszygton Str 13, 15 269 Białystok, Poland
    World J Gastroenterol 11:1115-21. 2005
    ..To assess the effect of non-selective ET(A/B) (LU 302872) and selective ET(A) (LU 302146) antagonist on pancreatic histology and ultrastructure of acinar cells in connection with trypsinogen activation in early caerulein-induced AP...
  74. ncbi Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris
    Theo Papakonstantinou
    ARC Special Research Centre for Green Chemistry, Building 75, Monash University, Clayton, Victoria 3800, Australia
    Protein Expr Purif 64:131-8. 2009
    ..chromatography, and for the removal of the FLAG affinity tag from purified recombinant human activin A by enteropeptidase, are described...
  75. ncbi Down-regulation of trypsinogen-2 expression by chemically modified tetracyclines: association with reduced cancer cell migration
    A Lukkonen
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Int J Cancer 86:577-81. 2000
    ..1 to 1.0 microM). DOXY specifically inhibited the activation of pro-MMP-9 and cell migration induced by enteropeptidase, a specific activator of trypsinogen...
  76. ncbi Mouse DESC1 is located within a cluster of seven DESC1-like genes and encodes a type II transmembrane serine protease that forms serpin inhibitory complexes
    John P Hobson
    Proteases and Tissue Remodeling Unit, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:46981-94. 2004
    ..Together, these data show that mouse DESC1 encodes a functional cell surface serine protease that may have important functions in the epidermis, oral, and reproductive epithelium...
  77. ncbi Production of active pediocin PA-1 in Escherichia coli using a thioredoxin gene fusion expression approach: cloning, expression, purification, and characterization
    Lucie Beaulieu
    Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montreal, QC 4P 2R2, Canada
    Can J Microbiol 53:1246-58. 2007
    ..0. The new production method, although not optimized, offers great potential for supporting further investigations on pediocin PA-1 and as a first-generation process for the production of pediocin PA-1 for high-value applications...
  78. ncbi Protamine-1 and -2 mRNA in round spermatids is associated with RNA-binding proteins
    Klaus Steger
    Institute of Veterinary Anatomy, Frankfurter Strasse 98, 35392 Giessen, Germany
    Histochem Cell Biol 117:227-34. 2002
    ..Furthermore, the presence of RNA-binding proteins within the coding sequence of mRNAs demands proteinase K treatment prior to cDNA synthesis, a compelling necessity for the study of gene expression...
  79. pmc Requirements of the engineered leader peptide of nisin for inducing modification, export, and cleavage
    Annechien Plat
    BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, Netherlands
    Appl Environ Microbiol 77:604-11. 2011
    ..Reducing the leader peptide to MSTKDFNLDLR led to 3- or 4-fold dehydration. Taken together, the FNLD box is crucial for inducing posttranslational modifications...
  80. ncbi Neurobin/TMPRSS11c, a novel type II transmembrane serine protease that cleaves fibroblast growth factor-2 in vitro
    Robert Stallmach
    Department of Biochemistry, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Biochem J 412:81-91. 2008
    ..These results indicate that neurobin is an authentic TTSP with trypsin-like activity and is able to process FGF-2 in vitro...
  81. pmc Serase-1B, a new splice variant of polyserase-1/TMPRSS9, activates urokinase-type plasminogen activator and the proteolytic activation is negatively regulated by glycosaminoglycans
    Yuushi Okumura
    Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, 3 18 15 Kuramoto cho, Tokushima 770 8503, Japan
    Biochem J 400:551-61. 2006
    ..Our results indicate that Serase-1B is a novel member of TTSPs that might be involved in uPA/plasmin-mediated proteolysis and possibly implicated in biological events such as fibrinolysis and tumour progression...
  82. ncbi The adrenal secretory serine protease AsP is a short secretory isoform of the transmembrane airway trypsin-like protease
    Immo A Hansen
    Endocrinology and Diabetes Unit, Department of Medicine, University of Wuerzburg, Germany
    Endocrinology 145:1898-905. 2004
    ..consisting of a short cytoplasmic domain, a transmembrane domain, a SEA (63-kDa sea urchin sperm protein, enteropeptidase, agrin) module, and a serine protease domain. The human homolog of MAT1 and RAT1 is the human AT (HAT)...
  83. ncbi Genetic recombinant expression and characterization of human augmenter of liver regeneration
    Chun fang Gao
    Department of Laboratory Medicine, Eastern Hepatobiliary Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China
    Dig Dis Sci 54:530-7. 2009
    ..To establish a highly effective prokaryotic recombinant expression system for human augmenter of liver regeneration (hALR) and to characterize the recombinant hALR both in vitro and in vivo...
  84. ncbi Impact of different modalities of continuous venovenous hemofiltration on sepsis-induced alterations in experimental pancreatitis
    Emre F Yekebas
    Department of Surgery, University Hospital Eppendorf, Hamburg, Germany
    Kidney Int 62:1806-18. 2002
    ..The impact of different treatment intensities of CVVH on immunologic and systemic alterations in experimental pancreatitis was evaluated...
  85. pmc Nuclear translocation of beta-dystroglycan reveals a distinctive trafficking pattern of autoproteolyzed mucins
    Maria Luisa Oppizzi
    California Pacific Medical Center Research Institute, 475 Brannan Street, Suite 220, San Francisco, CA 94107, USA
    Traffic 9:2063-72. 2008
    ..They also reveal an evolutionarily conserved role for SEA autoproteolysis, serving to enable independent functions of mucin transmembrane subunits, enacted by a shared and poorly understood pathway of segregated subunit trafficking...
  86. ncbi Purification and characterization of anthranilate synthase component I (TrpE) from Mycobacterium tuberculosis H37Rv
    Xiaohua Lin
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China
    Protein Expr Purif 64:8-15. 2009
    ..tuberculosis H37Rv TrpE. These results would be beneficial to the designing of novel anti-TB drugs with high potency and selectivity...
  87. ncbi Human anionic trypsinogen: properties of autocatalytic activation and degradation and implications in pancreatic diseases
    Zoltán Kukor
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, USA
    Eur J Biochem 270:2047-58. 2003
    ....
  88. ncbi Osteopontin, a novel substrate for matrix metalloproteinase-3 (stromelysin-1) and matrix metalloproteinase-7 (matrilysin)
    R Agnihotri
    Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074, USA
    J Biol Chem 276:28261-7. 2001
    ..We predict that interaction of MMPs with OPN at tumor and wound healing sites in vivo may be a mechanism of regulation of OPN bioactivity...
  89. ncbi Synthesis in Escherichia coli of avian reovirus core protein varsigmaA and its dsRNA-binding activity
    H S Yin
    Department of Veterinary Medicine, National Chung Hsing University, Taichung, 403, Taiwan
    Virology 266:33-41. 2000
    ..The results indicate that the expressed protein evarsigmaA has dsRNA-binding activity similar to that of varsigmaA obtained from infected cells, and its binding is sequence-independent...
  90. ncbi cDNA cloning and characterization of maize phosphoenolpyruvate carboxykinase, a bundle sheath cell-specific enzyme
    T Furumoto
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan
    Plant Mol Biol 41:301-11. 1999
    ..These results strongly suggest that the intact N-terminal extension may be involved in the regulation of PCK activity in vivo through some modification such as reversible phosphorylation...
  91. ncbi Characterization of immunoreactive trypsinogen activation peptide in urine in acute pancreatitis
    Ulf Petersson
    Department of Surgery, Malmo University Hospital, Lund University, Malmo, Sweden
    JOP 7:274-82. 2006
    ..Despite the extensive use of this original TAP assay, there is no characterization of the TAP-like immunoreactivity measured...
  92. pmc The guinea pig pancreas secretes a single trypsinogen isoform, which is defective in autoactivation
    Béla Ozsvári
    Department of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA, USA
    Pancreas 37:182-8. 2008
    ..The aim of the present study was to purify and clone the trypsinogen isoforms from the guinea pig pancreas and characterize their activation properties...
  93. pmc Fusion expression of pedA gene to obtain biologically active pediocin PA-1 in Escherichia coli
    Shan na Liu
    School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    J Zhejiang Univ Sci B 12:65-71. 2011
    ..The recombinant pediocin PA-1 from the soluble fraction attained 384 AU from 1 ml culture medium of E. coli (pPA003PED2). Therefore, biologically active pediocin PA-1 could be obtained by these two hybrid gene expression methods...
  94. ncbi Human angiogenin fused to human CD30 ligand (Ang-CD30L) exhibits specific cytotoxicity against CD30-positive lymphoma
    M Huhn
    Fraunhofer IME, Department of Pharmaceutical Product Development, 52074 Aachen, Germany
    Cancer Res 61:8737-42. 2001
    ..This is the first report on the specific cytotoxicity of a recombinant completely human fusion toxin with possibly largely reduced immunogenicity for the treatment of CD30-positive malignancies...
  95. ncbi Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria
    Chun Li
    Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, Breivika, N 9037 Tromsø, Norway
    Dev Comp Immunol 34:286-92. 2010
    ..coli suggest that both recombinant SpStrongylocins 1 and 2 conduct their antibacterial activity by intracellular killing mechanisms because no increase in membrane permeability was detected...
  96. ncbi Construction of heat-inducible expression vector of Corynebacterium glutamicum and C. ammoniagenes: fusion of lambda operator with promoters isolated from C. ammoniagenes
    Jong Uk Park
    Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yongin 449 791, Korea
    J Microbiol Biotechnol 18:639-47. 2008
    ..glutamicum was tried by temperature shift-up. The results indicated that the constructed vectors (pCeHEMG) can be successfully used in the expression and regulation of foreign genes in corynebacteria...
  97. ncbi Identification and expression of the cDNA-encoding human mesotrypsin(ogen), an isoform of trypsin with inhibitor resistance
    C N Nyaruhucha
    Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan
    J Biol Chem 272:10573-8. 1997
    ..It is concluded that the cloned cDNA encodes human mesotrypsinogen, a unique isoform of trypsinogen with inhibitor resistance...
  98. ncbi Purification and characterization of a functionally active Mycobacterium tuberculosis prephenate dehydrogenase
    Shengfeng Xu
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China
    Protein Expr Purif 49:151-8. 2006
    ..tuberculosis PDH is a monofunctional protein...
  99. ncbi [Cloning and expression of the cry1Ac-tchiB fusion gene from Bacillus thuringinesis and Tobacco and its insecticidal synergistic effect]
    Xue Zhi Ding
    College of Life Sciences of Hunan Normal University, Key Laboratory for Molecular Biology of Microorganism of Hunan province, Changsha 410081, China
    Wei Sheng Wu Xue Bao 47:1002-8. 2007
    ..The study will enhances the toxicity of B. thuringiensis Cry toxins protein and makes a ground for constructing the fusion genes of B. thuringiensis cry gene and other foreign toxin genes and the recombinant strain with high toxicity...
  100. pmc Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein
    B Thomas Bäckström
    Malaghan Institute of Medical Research, Wellington, New Zealand
    BMC Biotechnol 7:3. 2007
    ..As an alternative, we genetically engineered Escherichia coli to produce biopolyester (polyhdroxyalkanoate=PHA) granules displaying diagnostically relevant antigens in their native conformation and suitable for FACS analysis...
  101. ncbi BACE1 is a newly discovered protein secreted by the pancreas which cleaves enteropeptidase in vitro
    Albrecht Hoffmeister
    Department of Internal Medicine II, University of Leipzig, Leipzig, Germany
    JOP 10:501-6. 2009
    ..Strong BACE1 expression has also been described in pancreatic tissue...

Research Grants9

  1. Fluorogenic Assays for Factor Vlla and Tissue Factor
    RICHARD JENNY; Fiscal Year: 2006
    ..It is anticipated that by the end of the Phase-ll project period these assays will be ready to market for research and pre-clinical applications. ..
  2. ENTEROKINASE--AN INTESTINAL DIGESTIVE PROTEASE
    J Sadler; Fiscal Year: 2003
    b>Enteropeptidase (also known as enterokinase) is a protease of the intestinal brush border that specifically cleaves trypsinogen to yield trypsin, which then cleaves and activates other pancreatic zymogens...
  3. Mechanism and Therapies for HAART Induced Diarrhea
    Tibor Sipos; Fiscal Year: 2001
    ..proposed commercial application: not available ..
  4. STRUCTURE/FUNCTION OF SURFACTANT PROTEIN D
    Erika Crouch; Fiscal Year: 2006
    ..As in the past, secreted recombinant proteins with novel structural attributes will be characterized and utilized for collaborative studies of SP-D function. [unreadable] [unreadable]..
  5. Bionanotechnology for Breast and Prostate Cancer
    Elizabeth Singer; Fiscal Year: 2007
    ..Our preliminary results show that a device displaying multiple copies of the growth factor ligand heregulin can bind to cancer cells. ..
  6. A Global Test of Hemostatic Risk
    RICHARD JENNY; Fiscal Year: 2008
    ..Furthermore, this test may facilitate the administration and monitoring of individualized therapeutic treatment regimens in the thrombotic and hemorrhagic patient populations. [unreadable] [unreadable] [unreadable]..
  7. Structure and Function of ADAMTS13 Metalloprotease
    X Long Zheng; Fiscal Year: 2009
    ....