metalloendopeptidases

Summary

Summary: ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.

Top Publications

  1. ncbi A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells
    R A Black
    Immunex Corporation, Seattle, Washington 98101, USA
    Nature 385:729-33. 1997
  2. ncbi Changing views of the role of matrix metalloproteinases in metastasis
    A F Chambers
    Department of Oncology, University of Western Ontario, and London Regional Cancer Centre, Canada
    J Natl Cancer Inst 89:1260-70. 1997
  3. ncbi Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice
    R D Hautamaki
    Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, MO 63110, USA
    Science 277:2002-4. 1997
  4. pmc Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells
    Brian Head
    Department of Biological Chemistry, University of California, Los Angeles, 90095, USA
    J Cell Biol 187:959-66. 2009
  5. ncbi Tissue inhibitors of metalloproteinases: evolution, structure and function
    K Brew
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101, USA
    Biochim Biophys Acta 1477:267-83. 2000
  6. pmc Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1
    Sarah Ehses
    Institute for Genetics, University of Cologne, Germany
    J Cell Biol 187:1023-36. 2009
  7. ncbi A matrix metalloproteinase expressed on the surface of invasive tumour cells
    H Sato
    Department of Molecular Virology and Oncology, School of Medicine, Kanazawa University, Ishikawa, Japan
    Nature 370:61-5. 1994
  8. ncbi An essential role for ectodomain shedding in mammalian development
    J J Peschon
    Immunex Corporation, Seattle, WA 98101, USA
    Science 282:1281-4. 1998
  9. ncbi The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function
    Chaoqun Yao
    VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 132:1-16. 2003
  10. ncbi Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme
    P Reddy
    Department of Cell Sciences, Immunex Corporation, Seattle, Washington 98101, USA
    J Biol Chem 275:14608-14. 2000

Research Grants

Detail Information

Publications292 found, 100 shown here

  1. ncbi A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells
    R A Black
    Immunex Corporation, Seattle, Washington 98101, USA
    Nature 385:729-33. 1997
    ..Our results should facilitate the development of therapeutically useful inhibitors of TNF-alpha release, and they indicate that an important function of adamalysins may be to shed cell-surface proteins...
  2. ncbi Changing views of the role of matrix metalloproteinases in metastasis
    A F Chambers
    Department of Oncology, University of Western Ontario, and London Regional Cancer Centre, Canada
    J Natl Cancer Inst 89:1260-70. 1997
    ..Further clarification of the mechanisms by which MMPs regulate growth of primary and metastatic tumors will be important in the development of novel therapeutic strategies against metastases...
  3. ncbi Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice
    R D Hautamaki
    Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, MO 63110, USA
    Science 277:2002-4. 1997
    ..Thus, macrophage elastase is probably sufficient for the development of emphysema that results from chronic inhalation of cigarette smoke...
  4. pmc Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells
    Brian Head
    Department of Biological Chemistry, University of California, Los Angeles, 90095, USA
    J Cell Biol 187:959-66. 2009
    ..Inducible cleavage provides a mechanism for quality control because proteolytic inactivation of OPA1 promotes selective removal of defective mitochondrial fragments by preventing their fusion with the mitochondrial network...
  5. ncbi Tissue inhibitors of metalloproteinases: evolution, structure and function
    K Brew
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101, USA
    Biochim Biophys Acta 1477:267-83. 2000
    ....
  6. pmc Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1
    Sarah Ehses
    Institute for Genetics, University of Cologne, Germany
    J Cell Biol 187:1023-36. 2009
    ..Our findings link distinct peptidases to constitutive and induced OPA1 processing and shed new light on the pathogenesis of neurodegenerative disorders associated with mutations in m-AAA protease subunits...
  7. ncbi A matrix metalloproteinase expressed on the surface of invasive tumour cells
    H Sato
    Department of Molecular Virology and Oncology, School of Medicine, Kanazawa University, Ishikawa, Japan
    Nature 370:61-5. 1994
    ..The new metalloproteinase may thus trigger invasion by tumour cells by activating pro-gelatinase A on the tumour cell surface...
  8. ncbi An essential role for ectodomain shedding in mammalian development
    J J Peschon
    Immunex Corporation, Seattle, WA 98101, USA
    Science 282:1281-4. 1998
    ..The phenotype of mice lacking TACE suggests an essential role for soluble TGFalpha in normal development and emphasizes the importance of protein ectodomain shedding in vivo...
  9. ncbi The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function
    Chaoqun Yao
    VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
    Mol Biochem Parasitol 132:1-16. 2003
    ..The MSPs exquisitely demonstrate the multiple levels of post-transcriptional gene regulation that occur in Leishmania sp. and other trypanosomatid protozoa...
  10. ncbi Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme
    P Reddy
    Department of Cell Sciences, Immunex Corporation, Seattle, Washington 98101, USA
    J Biol Chem 275:14608-14. 2000
    ..IL-1R-II shedding required the cysteine-rich domain of TACE as well as the catalytic domain, whereas TNF and p75 TNFR shedding required only the tethered TACE catalytic domain...
  11. ncbi Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences
    A B Taylor
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 9:615-25. 2001
    ....
  12. ncbi Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha
    M L Moss
    Department of Molecular Biochemistry, Glaxo Wellcome Research and Development Inc, Research Triangle Park, North Carolina 27709, USA
    Nature 385:733-6. 1997
    ..The rTACE provides a readily available source of enzyme to help in the search for new anti-inflammatory agents that target the final processing stage of TNF-alpha production...
  13. ncbi TIMP-3 is a potent inhibitor of aggrecanase 1 (ADAM-TS4) and aggrecanase 2 (ADAM-TS5)
    M Kashiwagi
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101, USA
    J Biol Chem 276:12501-4. 2001
    ..This provides a starting point for developing a biologically available aggrecanase inhibitor suitable for the treatment of arthritis...
  14. ncbi Reduced angiogenesis and tumor progression in gelatinase A-deficient mice
    T Itoh
    Shionogi Institute for Medical Science, Shionogi and Co, Ltd, Osaka, Japan
    Cancer Res 58:1048-51. 1998
    ..014 and P < 0.0015, respectively). These results indicated that host-derived gelatinase A plays an important role in angiogenesis and tumor progression, suggesting the usefulness of gelatinase A inhibitors for anticancer chemotherapy...
  15. ncbi Cardiac hypertrophy is inhibited by antagonism of ADAM12 processing of HB-EGF: metalloproteinase inhibitors as a new therapy
    Masanori Asakura
    Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Osaka, Japan
    Nat Med 8:35-40. 2002
    ..These data suggest that shedding of HB-EGF by ADAM12 plays an important role in cardiac hypertrophy, and that inhibition of HB-EGF shedding could be a potent therapeutic strategy for cardiac hypertrophy...
  16. ncbi Membrane type I matrix metalloproteinase usurps tumor growth control imposed by the three-dimensional extracellular matrix
    Kevin B Hotary
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 114:33-45. 2003
    ..These observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D ECM...
  17. ncbi Defective prelamin A processing and muscular and adipocyte alterations in Zmpste24 metalloproteinase-deficient mice
    Alberto M Pendas
    Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Nat Genet 31:94-9. 2002
    ..These results indicate that prelamin A is a specific substrate for Zmpste24 and demonstrate the usefulness of genetic approaches for identifying the in vivo substrates of proteolytic enzymes...
  18. pmc A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification
    Basma El Yacoubi
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
    EMBO J 30:882-93. 2011
    ..We also showed that YaeZ, a YgjD paralog, is required for YgjD function in vivo in bacteria. This work lays the foundation for understanding the pleiotropic role of this universal protein family...
  19. ncbi Bone morphogenetic protein-1: the type I procollagen C-proteinase
    E Kessler
    Maurice and Gabriela Goldschleger Eye Research Institute, Tel Aviv University Sackler Faculty of Medicine, Sheba Medical Center, Tel Hashomer, Israel
    Science 271:360-2. 1996
    ..Here it is shown that BMP-1 and PCP are identical. This demonstration of enzymatic activity for a BMP-1/TLD-like protein links an enzyme involved in matrix deposition to genes involved in pattern formation...
  20. ncbi Leishmania GP63 alters host signaling through cleavage-activated protein tyrosine phosphatases
    Maria Adelaida Gomez
    Department of Experimental Medicine, McGill University, Montreal, Quebec, Canada
    Sci Signal 2:ra58. 2009
    ....
  21. ncbi von Willebrand factor-cleaving protease in thrombotic thrombocytopenic purpura and the hemolytic-uremic syndrome
    M Furlan
    Central Hematology Laboratory, University Hospital, Bern, Switzerland
    N Engl J Med 339:1578-84. 1998
    ..We investigated the prevalence of von Willebrand factor-cleaving protease deficiency in patients with familial and nonfamilial forms of these disorders...
  22. pmc Proteomic analyses reveal an acidic prime side specificity for the astacin metalloprotease family reflected by physiological substrates
    Christoph Becker-Pauly
    Cell and Matrix Biology, Johannes Gutenberg University, Johannes von Müller Weg 6, D 55128 Mainz, Germany
    Mol Cell Proteomics 10:M111.009233. 2011
    ..Moreover, the novel substrate FGF-19 was validated biochemically and shown to exhibit altered biological activity after meprin processing...
  23. ncbi Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage
    J M Gutierrez
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    Biochimie 82:841-50. 2000
    ....
  24. ncbi Carboxypeptidase M expressed by human bone marrow cells cleaves the C-terminal lysine of stromal cell-derived factor-1alpha: another player in hematopoietic stem/progenitor cell mobilization?
    Leah Marquez-Curtis
    Research and Development, Canadian Blood Services, Edmonton, Alberta, Canada
    Stem Cells 26:1211-20. 2008
    ..We suggest that cleavage of the C-terminal lysine residue of SDF-1alpha by CPM leads to attenuated chemotactic responses and could facilitate G-CSF-induced mobilization of HSPC from BM to peripheral blood...
  25. pmc gp63 in stable cationic liposomes confers sustained vaccine immunity to susceptible BALB/c mice infected with Leishmania donovani
    Swati Bhowmick
    Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Kolkata 700032, India
    Infect Immun 76:1003-15. 2008
    ..Our results demonstrated that stable cationic liposomes containing gp63 acted as a potent adjuvant for protein antigen to induce long-term protection against L. donovani that represents an alternative to DNA vaccination...
  26. pmc The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A
    Madhusudhan Srinivasan
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA
    EMBO J 30:873-81. 2011
    ..It remains to be determined whether all reported defects of KEOPS/EKC mutants can be attributed to the lack of t6A, or whether the complex has multiple functions...
  27. pmc Insulin-like growth factor 1 treatment extends longevity in a mouse model of human premature aging by restoring somatotroph axis function
    Guillermo Mariño
    Departamento de Bioquímica y Biología Molecular and Biología Funcional, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Proc Natl Acad Sci U S A 107:16268-73. 2010
    ..Our findings highlight the importance of IGF/GH balance in longevity and may be of therapeutic interest for devastating human progeroid syndromes associated with nuclear envelope abnormalities...
  28. pmc Loss of m-AAA protease in mitochondria causes complex I deficiency and increased sensitivity to oxidative stress in hereditary spastic paraplegia
    Luigia Atorino
    Human Molecular Genetics Unit, DIBIT San Raffaele Scientific Institute, 20132 Milan, Italy
    J Cell Biol 163:777-87. 2003
    ..These results shed new light on the molecular pathogenesis of HSP and functionally link AFG3L2 to this neurodegenerative disease...
  29. pmc TACE cleavage of proamphiregulin regulates GPCR-induced proliferation and motility of cancer cells
    Andreas Gschwind
    Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 22:2411-21. 2003
    ..Thus, TACE can function as an effector of GPCR-mediated signalling and represents a key element of the cellular receptor cross-talk network...
  30. ncbi Spastic paraplegia and OXPHOS impairment caused by mutations in paraplegin, a nuclear-encoded mitochondrial metalloprotease
    G Casari
    Telethon Institute of Genetics and Medicine, Milan, Italy
    Cell 93:973-83. 1998
    ..Analysis of muscle biopsies from two patients carrying Paraplegin mutations showed typical signs of mitochondrial OXPHOS defects, thus suggesting a mechanism for neurodegeneration in HSP-type disorders...
  31. pmc Meprins, membrane-bound and secreted astacin metalloproteinases
    Erwin E Sterchi
    Institute of Biochemistry and Molecular Biology, University of Berne, Buehlstrasse 28, CH 3012 Berne, Switzerland
    Mol Aspects Med 29:309-28. 2008
    ..The in vivo substrates of meprins are unknown, but the abundant expression of these proteinases in the epithelial cells of the intestine, kidney and skin provide clues to their functions...
  32. ncbi Partial purification and characterization of a protease from human plasma cleaving von Willebrand factor to fragments produced by in vivo proteolysis
    M Furlan
    Central Hematology Laboratory of the University, Inselspital, Bern, Switzerland
    Blood 87:4223-34. 1996
    ..The size, amino acid composition, and amino terminal sequence of the reduced fragments confirmed that the peptide bond 842Tyr-843Met had been cleaved, ie, the same bond that has been proposed to be cleaved in vivo...
  33. ncbi MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover
    K Holmbeck
    MMP Unit, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA
    Cell 99:81-92. 1999
    ....
  34. ncbi The neprilysin (NEP) family of zinc metalloendopeptidases: genomics and function
    A J Turner
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds, UK
    Bioessays 23:261-9. 2001
    ..These model organisms also provide convenient systems for examining cell-specific expression, developmental and functional roles of this peptidase family, and reveal the power of functional genomics...
  35. pmc Yeast homolog of a cancer-testis antigen defines a new transcription complex
    Elena Kisseleva-Romanova
    Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, Gif sur Yvette, Paris, France
    EMBO J 25:3576-85. 2006
    ..Functional complementation experiments indicate that the transcriptional function of this set of genes is conserved throughout evolution...
  36. pmc The Leishmania surface protease GP63 cleaves multiple intracellular proteins and actively participates in p38 mitogen-activated protein kinase inactivation
    Maxime Halle
    Rosalind and Morris Goodman Cancer Centre, Department of Biochemistry, McGill University, Montreal, Quebec H3A 1A3, Canada
    J Biol Chem 284:6893-908. 2009
    ..Our results establish that GP63 plays a central role in a number of hostcell molecular events that likely contribute to the infectivity of Leishmania...
  37. pmc Characterization of Leishmania donovani antigens encapsulated in liposomes that induce protective immunity in BALB/c mice
    Farhat Afrin
    Infectious Diseases Group, Indian Institute of Chemical Biology, Calcutta 700032, India
    Infect Immun 70:6697-706. 2002
    ..donovani...
  38. ncbi Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis
    D Pan
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720 3200, USA
    Cell 90:271-80. 1997
    ..We provide genetic and biochemical evidence that Notch is an in vivo substrate for the KUZ protease, and that this cleavage may be part of the normal biosynthesis of functional Notch proteins...
  39. pmc Variation in extracellular protease production among clinical isolates of Staphylococcus aureus due to different levels of expression of the protease repressor sarA
    Anna Karlsson
    Microbiology and Tumor Biology Center MTC, Karolinska Institutet, S 17177 Stockholm, Sweden
    Infect Immun 70:4239-46. 2002
    ..Our results suggest that the protease genes are conserved among clinical S. aureus strains and that the level of SigB-dependent expression of the protease repressor sarA determines the level of protease production in each strain...
  40. ncbi Soluble metalloendopeptidases and neuroendocrine signaling
    Corie N Shrimpton
    Baker Medical Research Institute, Melbourne, Australia 8008
    Endocr Rev 23:647-64. 2002
    ..The thermolysin-like family of metalloendopeptidases involved in the extracellular processing of neuroendocrine and cardiovascular peptides are of particular ..
  41. ncbi The cell surface: the stage for matrix metalloproteinase regulation of migration
    Motoharu Seiki
    Division of Cancer Cell Research, Institute of Medial Science, University of Tokyo, Japan
    Curr Opin Cell Biol 14:624-32. 2002
    ..In particular, some matrix metalloproteinases that associate with the cell surface have arisen as intriguing regulators of cellular functions, including migration...
  42. pmc ECM regulates MT1-MMP localization with beta1 or alphavbeta3 integrins at distinct cell compartments modulating its internalization and activity on human endothelial cells
    Beatriz G Galvez
    Departamento de Inmunologia, Hospital de la Princesa, C Diego de Leon 62, 28006 Madrid, Spain
    J Cell Biol 159:509-21. 2002
    ..These data show a novel mechanism by which ECM regulates MT1-MMP association with beta1 or alphavbeta3 integrins at distinct cellular compartments, thus modulating its internalization, activity, and function on human ECs...
  43. pmc The metalloprotease meprin β generates amino terminal-truncated amyloid β peptide species
    Jessica Bien
    Institute of Pathobiochemistry, University Medical Centre of the Johannes Gutenberg University of Mainz, 55128 Mainz, Germany
    J Biol Chem 287:33304-13. 2012
    ..This enzymatic activity of meprin β on APP and Aβ generation was also observed in the absence of BACE1/2 activity using a β-secretase inhibitor and BACE knock-out cells, indicating that meprin β acts independently of β-secretase...
  44. pmc Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex
    Arnaud Hecker
    Institut de Genetique et Microbiologie, Universite Paris Sud, IFR115 UMR8621 CNR, Orsay, France
    EMBO J 27:2340-51. 2008
    ..Direct interaction between Kae1p and Bud32p in yeast is required both for the transcription and the telomere homeostasis function of EKC/KEOPS...
  45. pmc Mechanisms of vascular damage by hemorrhagic snake venom metalloproteinases: tissue distribution and in situ hydrolysis
    Cristiani Baldo
    Laboratorio de Imunopatologia, Instituto Butantan, Sao Paulo, Sao Paulo, Brasil
    PLoS Negl Trop Dis 4:e727. 2010
    ....
  46. ncbi Protein rigidity and thermophilic adaptation
    Sebastian Radestock
    Mathematisch Naturwissenschaftliche Fakultät, Institut fur Pharmazeutische und Medizinische Chemie, Heinrich Heine Universitat Dusseldorf, Germany
    Proteins 79:1089-108. 2011
    ....
  47. ncbi A novel form of NF-kappaB is induced by Leishmania infection: involvement in macrophage gene expression
    David J Gregory
    Centre for the Study of Host Resistance, Research Institute of the McGill University Health Centre, Montreal, Canada
    Eur J Immunol 38:1071-81. 2008
    ..Viannia) braziliensis, but not the non-pathogenic L. tarentolae or treatment with IFN-gamma. These results indicate a novel mechanism by which a pathogen can subvert a macrophage's regulatory pathways to alter NF-kappaB activity...
  48. ncbi Role of meprin A in renal tubular epithelial cell injury
    C Herzog
    Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
    Kidney Int 71:1009-18. 2007
    ..Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of AKI...
  49. pmc Meprinα transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration
    Petra Minder
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buehlstrasse 28, CH 3012 Bern, Switzerland
    J Biol Chem 287:35201-11. 2012
    ..In conclusion, the implication of meprinα in the EGFR/MAPK signaling pathway indicates a role of meprinα in colorectal cancer progression...
  50. ncbi Emerging roles of mitochondrial proteases in neurodegeneration
    Paola Martinelli
    Laboratory of Genetic and Molecular Pathology, Istituto Neurologico C Besta, Milan, Italy
    Biochim Biophys Acta 1797:1-10. 2010
    ..Here we review our current knowledge on how disturbances of the mitochondrial proteolytic system affect neuronal maintenance and axonal function...
  51. pmc Enhanced activity of meprin-α, a pro-migratory and pro-angiogenic protease, in colorectal cancer
    Daniel Lottaz
    Department of Rheumatology, Clinical Immunology and Allergology, Inselspital, University Hospital of Bern, Bern, Switzerland
    PLoS ONE 6:e26450. 2011
    ..By virtue of its pro-migratory and pro-angiogenic activity, meprin-α may promote tumor progression in colorectal cancer...
  52. ncbi Development and evaluation of polymerase chain reaction assay to detect Burkholderia genus and to differentiate the species in clinical specimens
    Jeyanthi Suppiah
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    FEMS Microbiol Lett 306:9-14. 2010
    ..However, the real-time PCR was able to detect and differentiate the genus and species in single duplex assay...
  53. ncbi The role of CpG ODN in enhancement of immune response and protection in BALB/c mice immunized with recombinant major surface glycoprotein of Leishmania (rgp63) encapsulated in cationic liposome
    Mahmoud R Jaafari
    School of Pharmacy, Biotechnology Research Center and Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
    Vaccine 25:6107-17. 2007
    ..The results indicate that co-encapsulation of CpG ODN in liposomes improves the immunogenicity of Leishmania antigen...
  54. pmc Role of meprins to protect ileal mucosa of Crohn's disease patients from colonization by adherent-invasive E. coli
    Emilie Vazeille
    Clermont Universite, JE2526 Université d Auvergne, Clermont Ferrand, France
    PLoS ONE 6:e21199. 2011
    ..Thus, decreased levels of protective meprins as observed in CD patients may contribute to increased AIEC colonization...
  55. ncbi ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan
    K Kuno
    Department of Molecular Membrane Biology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan
    FEBS Lett 478:241-5. 2000
    ..In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo...
  56. ncbi Carboxypeptidase M and kinin B1 receptors interact to facilitate efficient b1 signaling from B2 agonists
    Xianming Zhang
    Department of Pharmacology, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60612, USA
    J Biol Chem 283:7994-8004. 2008
    ..Thus, a close relationship of B1Rs and CPM on the membrane is required for efficiently generating B1R signals, which play important roles in inflammation...
  57. ncbi Nardilysin regulates axonal maturation and myelination in the central and peripheral nervous system
    Mikiko Ohno
    Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    Nat Neurosci 12:1506-13. 2009
    ..On the basis of these data, we propose that NRDc regulates axonal maturation and myelination in the CNS and PNS, in part, through the modulation of NRG1 shedding...
  58. pmc Whole-exome sequencing identifies homozygous AFG3L2 mutations in a spastic ataxia-neuropathy syndrome linked to mitochondrial m-AAA proteases
    Tyler Mark Pierson
    NIH Undiagnosed Diseases Program, National Institutes of Health Office of Rare Diseases Research and National Human Genome Research Institute, Bethesda, Maryland, United States of America
    PLoS Genet 7:e1002325. 2011
    ..These findings expand the phenotype associated with AFG3L2 mutations and suggest that AFG3L2-related disease should be considered in the differential diagnosis of spastic ataxias...
  59. ncbi Endothelial tubulogenesis within fibrin gels specifically requires the activity of membrane-type-matrix metalloproteinases (MT-MMPs)
    Marc A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    J Cell Sci 115:3427-38. 2002
    ..Therefore MT-MMPs and specifically MT1-MMP are likely candidates for involvement during endothelial tubulogenesis within a fibrin matrix, and thus their blockade may be a viable strategy for inhibition of angiogenesis...
  60. pmc Immunogenicity and efficacy of single antigen Gp63, polytope and polytopeHSP70 DNA vaccines against visceral Leishmaniasis in experimental mouse model
    Rakhee Sachdeva
    Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
    PLoS ONE 4:e7880. 2009
    ..Therefore, our study indicates that polytope DNA vaccine is a feasible, practical and effective approach for visceral leishmaniasis...
  61. ncbi Proteomic comparison of membrane and extracellular proteins from invasive (PAO1) and cytotoxic (6206) strains of Pseudomonas aeruginosa
    Amanda S Nouwens
    Australian Proteome Analysis Facility, Sydney, Australia
    Proteomics 2:1325-46. 2002
    ..These studies will aid in elucidating the differences between invasive and cytotoxic P. aeruginosa at the proteome level...
  62. pmc The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to host cells
    Thomas E Grys
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 1300 University Ave, Room 481 MSC, Madison, WI 53706, USA
    Infect Immun 73:1295-303. 2005
    ..Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycoproteins, and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface...
  63. pmc Modulation of neutrophil function by a secreted mucinase of Escherichia coli O157:H7
    Rose L Szabady
    Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
    PLoS Pathog 5:e1000320. 2009
    ..StcE may contribute to inflammation and tissue destruction by mediating inappropriate neutrophil adhesion and activation...
  64. pmc Fibronectin binding and proteolytic degradation by Leishmania and effects on macrophage activation
    Manjusha M Kulkarni
    Division of Infectious Diseases and Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA
    Infect Immun 76:1738-47. 2008
    ....
  65. ncbi News from an ancient world: two novel astacin metalloproteases from the horseshoe crab
    Christoph Becker-Pauly
    Institute of Zoology, Cell and Matrix Biology, Johannes Gutenberg University, Mainz, Germany
    J Mol Biol 385:236-48. 2009
    ..For example, LAST_MAM is the only astacin protease known so far that is active in its zymogen form, indicating that the presence of the N-terminal propeptide does not prevent proteolytic activity...
  66. ncbi Membrane type 4 matrix metalloproteinase (MMP17) has tumor necrosis factor-alpha convertase activity but does not activate pro-MMP2
    W R English
    School of Biological Sciences, University of East Anglia, University Plain, Norwich, Norfolk NR4 7TJ, United Kingdom
    J Biol Chem 275:14046-55. 2000
    ..MT4-MMP was detected by Western blot in monocyte/macrophage cell lines which in combination with its fibrinolytic and TNFalpha-converting activity suggests a role in inflammation...
  67. pmc The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1
    S W Li
    Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 93:5127-30. 1996
    ..4.24.19), an enzyme that is essential for the processing of procollagens to fibrillar collagens. The results demonstrate that procollagen C-proteinase is identical to BMP-1...
  68. pmc MEP1A allele for meprin A metalloprotease is a susceptibility gene for inflammatory bowel disease
    S Banerjee
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA
    Mucosal Immunol 2:220-31. 2009
    ..Collectively, the data implicate MEP1A as a UC susceptibility gene and indicate that decreased meprin-alpha expression is associated with intestinal inflammation in IBD patients and in a mouse experimental model of IBD...
  69. ncbi Tobacco smoke-induced lung cell proliferation mediated by tumor necrosis factor alpha-converting enzyme and amphiregulin
    Hassan Lemjabbar
    Biomedical Sciences Program, Cardiovascular Research Institute and Department of Anatomy, University of California, San Francisco, California 94143, USA
    J Biol Chem 278:26202-7. 2003
    ..These results shed light on the pathogenesis of lung cancer, suggest novel drug targets for the reduction of cancer risk in smokers, and provide insight into how EGFR integrates responses to diverse noxious stimuli...
  70. ncbi A novel proteolytic cleavage involved in Notch signaling: the role of the disintegrin-metalloprotease TACE
    C Brou
    Unité de Biologie Moléculaire de l Expression Génique, URA 1773 CNRS, Institut Pasteur, Paris, France
    Mol Cell 5:207-16. 2000
    ..Furthermore, experiments carried out on TACE-/- bone marrow-derived monocytic precursor cells suggest that this metalloprotease plays a prominent role in the activation of the Notch pathway...
  71. pmc TIMP-2 is required for efficient activation of proMMP-2 in vivo
    Z Wang
    Roswell Park Cancer Institute, Department of Molecular and Cellular Biology, Buffalo, New York 16263, USA
    J Biol Chem 275:26411-5. 2000
    ..Mutant mice were overtly normal, viable, and fertile on the C57BL/6 background, indicating that both TIMP-2 and activated proMMP-2 are dispensable for normal development...
  72. pmc Overlapping and enzyme-specific contributions of matrix metalloproteinases-9 and -12 in IL-13-induced inflammation and remodeling
    Sophie Lanone
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Clin Invest 110:463-74. 2002
    ..The also demonstrate that MMPs-9 and -12 play different roles in the generation of IL-13-induced inflammation, with MMP-9 inhibiting neutrophil accumulation and MMP-12 contributing to the accumulation of eosinophils and macrophages...
  73. pmc Human meprin alpha and beta homo-oligomers: cleavage of basement membrane proteins and sensitivity to metalloprotease inhibitors
    Markus N Kruse
    Institute of Zoophysiology, University of Munster, Hindenburgplatz 55, D 48143 Munster, Germany
    Biochem J 378:383-9. 2004
    ....
  74. pmc Regulation of mitochondrial morphology through proteolytic cleavage of OPA1
    Naotada Ishihara
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
    EMBO J 25:2966-77. 2006
    ..Thus, mammalian mitochondrial function and morphology is regulated through processing of OPA1 in a DeltaPsi-dependent manner...
  75. pmc Laminopathies and the long strange trip from basic cell biology to therapy
    Howard J Worman
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    J Clin Invest 119:1825-36. 2009
    ..Here, we review the laminopathies and the long strange trip from basic cell biology to therapeutic approaches for these diseases...
  76. ncbi Matrix metalloproteinases: structures, evolution, and diversification
    I Massova
    Department of Chemistry, and Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48202 3489, USA
    FASEB J 12:1075-95. 1998
    ..Analysis indicates that a retrograde structure simplification may have accounted for the evolution of MMPs with simple domain constituents, such as matrilysin, from the larger and more elaborate enzymes...
  77. ncbi Fertilization defects in sperm from mice lacking fertilin beta
    C Cho
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Science 281:1857-9. 1998
    ..Fertilin could also have a direct role in sperm-zona binding or oviduct migration; alternatively, the effects on these functions could result from the absence of fertilin activity during spermatogenesis...
  78. pmc OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1L
    Zhiyin Song
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 178:749-55. 2007
    ..Cleavage at S2 is regulated by the i-AAA protease Yme1L. Our results suggest that mammalian cells have multiple pathways to control mitochondrial fusion through regulation of the spectrum of OPA1 isoforms...
  79. pmc Let it flow: Morpholino knockdown in zebrafish embryos reveals a pro-angiogenic effect of the metalloprotease meprin alpha2
    André Schütte
    Institute of Zoology, Cell and Matrix Biology, Johannes Gutenberg University, Mainz, Germany
    PLoS ONE 5:e8835. 2010
    ..The discovery of meprins alpha(1), alpha(2) and beta in teleost fish provided the basis for uncovering their physiological functions by gene silencing in vivo...
  80. ncbi Staphylococcus aureus: Staphylokinase
    Maria I Bokarewa
    Department of Rheumatology and Inflammation Research, Sahlgrenska University Hospital, Guldhedsgatan 10, SE 41346 Göteborg, Sweden
    Int J Biochem Cell Biol 38:504-9. 2006
    ..Our observations indicate that staphylokinase favours symbiosis of staphylococci with the host that makes it an important colonization factor...
  81. pmc Regulation of membrane-type matrix metalloproteinase 1 activity by dynamin-mediated endocytosis
    A Jiang
    Department of Pharmacology, University of Minnesota, 6-120 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 98:13693-8. 2001
    ..Thus, MT1-MMP is regulated by dynamin-dependent endocytosis in clathrin-coated pits through its cytoplasmic domain...
  82. pmc Shiga toxin B subunits induce VWF secretion by human endothelial cells and thrombotic microangiopathy in ADAMTS13-deficient mice
    Jing Huang
    Departments of Medicine, Biochemistry, and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Blood 116:3653-9. 2010
    ..These results demonstrate the existence of a novel Stx B-induced lipid raft-dependent signaling pathway in endothelial cells that may be responsible for some of the biological effects attributed previously to the cytotoxic Stx A subunit...
  83. pmc A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor
    K Fujimura-Kamada
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 136:271-85. 1997
    ..Ste24p and the proteins related to it define a new subfamily of proteins that are likely to function as intracellular, membrane-associated zinc metalloproteases...
  84. ncbi Role of the matrix metalloproteinase and plasminogen activator-plasmin systems in angiogenesis
    M S Pepper
    Department of Morphology, University Medical Center, Geneva, Switzerland
    Arterioscler Thromb Vasc Biol 21:1104-17. 2001
    ..Antiproteolysis, particularly in the context of angiogenesis, has become a key target in therapeutic strategies aimed at inhibiting tumor growth and other diseases associated with neovascularization...
  85. ncbi Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase
    Florence Friggi-Grelin
    Laboratoire de Genetique et Physiologie du Developpement, CNRS Université de la Méditerranée, Developmental Biology Institute of Marseille, Campus de Luminy, Case 907, F 13288 Marseille Cedex 9, France
    J Neurobiol 54:618-27. 2003
    ..We propose that TH-GAL4 will be useful for studies of the role of DA in behavior and disease models in Drosophila...
  86. ncbi A proteolytic transmembrane signaling pathway and resistance to beta-lactams in staphylococci
    H Z Zhang
    Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California at San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    Science 291:1962-5. 2001
    ..Compounds that disrupt this regulatory pathway could restore the activity of beta-lactam antibiotics against drug-resistant strains of staphylococci...
  87. ncbi Hepatocyte growth factor receptor, matrix metalloproteinase-11, tissue inhibitor of metalloproteinase-1, and fibronectin are up-regulated in papillary thyroid carcinoma: a cDNA and tissue microarray study
    Veli Matti Wasenius
    Department of Oncology, Helsinki University Central Hospital, FIN 00029 Helsinki, Finland
    Clin Cancer Res 9:68-75. 2003
    ....
  88. pmc Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis
    Katarina Wolf
    Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany
    J Cell Biol 160:267-77. 2003
    ....
  89. ncbi StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
    Wyndham W Lathem
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 45:277-88. 2002
    ..Cleavage of C1-INH by StcE could plausibly cause localized pro-inflammatory and coagulation responses resulting in tissue damage, intestinal oedema and thrombotic abnormalities...
  90. pmc Marimastat as maintenance therapy for patients with advanced gastric cancer: a randomised trial
    S R Bramhall
    Department of Surgery, Queen Elizabeth Hospital, Birmingham, UK
    Br J Cancer 86:1864-70. 2002
    ..The greatest benefit was observed in patients who had previously received chemotherapy. A further randomised study of marimastat in these patients is warranted...
  91. ncbi The ADAM gene family: surface proteins with adhesion and protease activity
    P Primakoff
    Department of Cell Biology and Anatomy, School of Medicine, University of California, Davis, CA 95616, USA
    Trends Genet 16:83-7. 2000
    ..We discuss the ADAM gene products with known functions that act in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis, embryonic TGF-alpha release and the inflammatory response...
  92. ncbi Identification and characterization of the fifth membrane-type matrix metalloproteinase MT5-MMP
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 274:8925-32. 1999
    ..Taken together, these properties serve to distinguish MT5-MMP as a versatile MT-MMP playing an important role in extracellular matrix remodeling events in the brain and during embryonic development...
  93. ncbi ADAM12 in human liver cancers: TGF-beta-regulated expression in stellate cells is associated with matrix remodeling
    Hélène Le Pabic
    INSERM U456, Detoxication et Reparation Tissulaire, Universite de Rennes I, France
    Hepatology 37:1056-66. 2003
    ..In conclusion, in liver cancers ADAM9 and ADAM12 expression is associated with tumor aggressiveness and progression...
  94. ncbi Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages
    S D Shapiro
    Department of Medicine, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
    J Biol Chem 268:23824-9. 1993
    ..HME is a unique human metalloproteinase that possesses elastolytic activity and is expressed in alveolar macrophages; it is therefore a candidate molecule for the causation of diseases characterized by damage to the extracellular matrix...
  95. pmc Transcription of quorum-sensing system genes in clinical and environmental isolates of Pseudomonas aeruginosa
    Ségolène Cabrol
    INSERM EMI 9933, Epidémiologie de la Résistance aux Anti infectieux, and AP HP Groupe Hospitalier Bichat Claude Bernard, 75877 Paris Cedex 18, France
    J Bacteriol 185:7222-30. 2003
    ....
  96. ncbi Analyzing the protease web in skin: meprin metalloproteases are activated specifically by KLK4, 5 and 8 vice versa leading to processing of proKLK7 thereby triggering its activation
    Anke Ohler
    Institute of Zoology, Cell and Matrix Biology, Johannes Gutenberg University, Mainz, Germany
    Biol Chem 391:455-60. 2010
    ..In summary, we demonstrate a specific interaction between meprin metalloproteases and kallikrein-related peptidases, revealing possible interactions within the proteolytic web...
  97. ncbi Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinases (TIMP)-2
    H Sato
    Department of Molecular Virology and Oncology, Kanazawa University, Japan
    FEBS Lett 393:101-4. 1996
    ..Tissue inhibitor of metalloproteinases (TIMP)-2 inhibited activation of progelatinase A by forming a stable complex with activated MT1-MMP...
  98. ncbi Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta
    H Nishimura
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Dev Biol 233:204-13. 2001
    ..Because the altered sperm protein expression must be responsible for the fertilization defects, our data suggest new models for the molecular basis of the affected steps in fertilization...
  99. ncbi Meprin metalloprotease expression and regulation in kidney, intestine, urinary tract infections and cancer
    Judith S Bond
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    FEBS Lett 579:3317-22. 2005
    ....
  100. ncbi Activation of progelatinase A (MMP-2) by neutrophil elastase, cathepsin G, and proteinase-3: a role for inflammatory cells in tumor invasion and angiogenesis
    P Shamamian
    Department of Surgery, S.A. Localio Laboratory for Surgical Research, New York University School of Medicine, New York, New York 10016, USA
    J Cell Physiol 189:197-206. 2001
    ..These findings indicate a novel role for PMN-mediated inflammation in a variety of tissue remodeling processes including tumor invasion and angiogenesis...
  101. ncbi Degradation of circulating von Willebrand factor and its regulator ADAMTS13 implicates secreted Bacillus anthracis metalloproteases in anthrax consumptive coagulopathy
    Myung Chul Chung
    National Center for Biodefense and Infectious Diseases, College of Sciences, George Mason University, 10900 University Boulevard, Manassas, VA 20110, USA
    J Biol Chem 283:9531-42. 2008
    ..Our results suggest a new mechanism of anthrax coagulopathy affecting the levels and functional activities of both VWF and its natural regulator ADAMTS13. This mechanism may contribute to hemorrhage and thrombosis typical in anthrax...

Research Grants62

  1. NEURAL REGULATION OF PANCREATIC FUNCTION
    KIMBERLY SAUNDERS KIRKWOOD; Fiscal Year: 2012
    ..Finally we will investigate pathways that terminate pancreatic inflammation by examining the role of metalloendopeptidases that degrade proinflammatory peptides on the cell surface and in endosomes...
  2. Meprin A Metalloproteinase in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Meprins, cell-surface and secreted oligomeric metalloendopeptidases of the 'astacin'family, are highly expressed at the brush-border membranes of the kidney proximal tubules...
  3. Mineralization of the matrix in disease and health
    PETER S ROWE; Fiscal Year: 2013
    ..Design PHEX synthetic peptides as tools to probe the role of ASARM peptides and PHEX in health and disease. ..
  4. Extracellular Matrix, Cocaine, and Memory
    Barbara A Sorg; Fiscal Year: 2013
    ..MMPs belong to a family of metalloendopeptidases that can direct changes in synaptic morphology via their effects on the extracellular matrix (ECM)...
  5. Nanocarrier formulated enzyme for the treatment of S. aureus infection
    GERARDO M CASTILLO; Fiscal Year: 2013
    ..We will determine and compare with commercially available antibiotics the effectiveness of our lyso formulation in vivo against MRSA. PharmaIN Corp. Confidential ..
  6. OCULAR FUNCTIONS & PEPTIDE HORMONE SYSTEMS
    James Ryan; Fiscal Year: 1991
    ....
  7. Nanocarrier with metal bridge for affinity based delivery of metal binding peptid
    Elijah Bolotin; Fiscal Year: 2009
    ..The product will contain an enzyme (lysostaphin) to digest bacterial cell walls and a nanocarrier drug delivery system that would make the enzyme stable to clear infections in blood and tissue of the patients. ..
  8. Mineralization and the Role of Matrixphosphoglycoprotein
    Peter Rowe; Fiscal Year: 2005
    ....
  9. PEPTIDE PROCESSING ENZYMES IN THE PLACENTA
    Sandra Reznik; Fiscal Year: 2002
    ..Specific aim 1 will test the hypothesis that two closely related metalloendopeptidases, endothelin converting enzyme-1 (ECE-1) and neutral endopeptidase (NEP), function in the regulation of ..
  10. LPS-PEPTIDE INTERACTION IN BLADDER INFLAMMATION
    Ricardo Saban; Fiscal Year: 2007
    ..Information generated by this study should elucidate whether bladder inflammatory responses, regardless of etiology, occur via a common mechanism or whether the endpoints are specific for each stimulus. ..
  11. EXTRACELLULAR PROCESSING OF CARTILAGE PROTEOGLYCANS
    John Sandy; Fiscal Year: 1991
    ..Such knowledge might suggest methods for the useful intervention in joint disease at the level of improving matrix repair by mature chondrocytes...
  12. HUMAN LUNG NEUTRAL METALLOENDOPEPTIDASE
    Bruce Marshall; Fiscal Year: 1993
    ....
  13. Neural EP24.15- A Model for Neuropeptidase Function
    MARC GLUCKSMAN; Fiscal Year: 2009
    ..15, of its isoforms, and of closely related metalloendopeptidases, with the goal of understandingthe prominence of EP24...
  14. LH ACTION IN OVARIAN CELL DIFFERENTIATION
    Joanne Richards; Fiscal Year: 2008
    ..Specific Aim II: Determine the function of AREG target genes that are uniquely expressed at high levels in ovulated COCs (compared to granulosa cells) and that impact ovulation. ..
  15. Molecular Evolution of Enterotoxigenic B fragilis
    AUGUSTO FRANCO MORA; Fiscal Year: 2004
    ..Our results will advance our understanding of the molecular pathogenesis of ETBF infections and will serve to delineate how ETBF originated during evolution. ..
  16. PHYSIOLOGY AND CLONING OF B FRAGILIS ENTEROTOXIN
    CYNTHIA SEARS; Fiscal Year: 2010
    ..Our studies may indicate that a common stool bacterium is oncogenic and contributes to colon cancer risk. If so, these data will lead to new public health approaches to the prevention of colon cancer. ..
  17. LH ACTION IN OVARIAN CELL DIFFERENTIATION
    Joanne Richards; Fiscal Year: 2007
    ..A related and longer term goal of the research is to identify and characterize the genes regulated by LH, prostaglandins (PGs), and C/EBPs that control key steps in the ovulatory process. ..
  18. The function of Runx1 in the ovary
    Misung Jo; Fiscal Year: 2007
    ..Such knowledge can be applied for promoting or inhibiting these critical facets of ovarian physiology, thereby allowing us to better manage fertility, infertility, and ovarian-based disorders. [unreadable] [unreadable]..
  19. Ovarian Follicular Developmental and Hormone Action
    Joanne Richards; Fiscal Year: 2007
    ..3) Determine the molecular, cellular and hormonal basis of the altered ovarian phenotype of the TAFII105 null mice. 4) Determine the function of Wnt-4 in the adult ovary. ..
  20. Protein Misprocessing in Krabbe Disease
    Christopher Eckman; Fiscal Year: 2007
    ..Further we propose to determine the subcellular localization of this increased activity to determine if the enzyme has reached its proper location and to monitor natural substrate cleavage as a prelude to in vivo testing. ..
  21. Targeting the PEX Domain of MT1-MMP: Novel Cancer Therapy
    Jian Cao; Fiscal Year: 2010
    ..I propose that the current project will help us to better understand cancer progression and lead to the development of novel basic tools for treatment of early stage cancer. ..
  22. Regulation of Lipid and Membrane Synthesis in Drosophila
    ROBERT RAWSON; Fiscal Year: 2009
    ..These hypotheses will be easier to test in the simpler fly model, thereby complementing rather than duplicating studies of SREBPs ongoing in other laboratories. ..
  23. MEPRINS-METALLOPROTEINASES OF THE KIDNEY AND INTESTINE
    Judith S Bond; Fiscal Year: 2010
    ....
  24. Protein Tranduction for Treatment of Krabbe Disease
    Christopher Eckman; Fiscal Year: 2004
    ..The lessons learned from these experiments may be useful for other diseases where delivery of a therapeutic protein may be desired, such as Alzheimer's disease and Parkinson's. ..
  25. CANDIDATE GENE FOR DIABETIC NEPHROPATHY--MEPRIN BETA
    JUDITH BOND; Fiscal Year: 2002
    ..These studies will establish whether the meprin-b gene is associated with susceptibility to DN in humans, and provide animal models to determine the role of this proteinase in normal kidney and in susceptibility to renal disease. ..
  26. Pathogen of Enterotoxigenic Bacteriodes Fragilis Infect
    CYNTHIA SEARS; Fiscal Year: 2003
    ..We hypothesize that ETBF is an under-recognized intestinal pathogen accounting for a portion of previously undiagnosed inflammatory intestinal infections in both children and adults. ..
  27. Consequences of Neonatal Hypoxia
    Nanduri Prabhakar; Fiscal Year: 2009
    ..Understanding the consequences of neonatal IH provide insights into how neonatal intermittent hypoxia caused by recurrent apneas affects the ventilation in premature infants. Experimental Animal Model: Rat Pups. ..
  28. Microsporidiosis:A Therapeutic Target
    Louis Weiss; Fiscal Year: 2003
    ....
  29. Mass Spectrometry in Systems Biology
    John Yates; Fiscal Year: 2003
    ..Topics emphasizing global measurements as well as highly specific and accurate molecular measurements will be presented. ..
  30. Arterial 5-HT Transporter Function
    Stephanie Watts; Fiscal Year: 2009
    ..abstract_text> ..
  31. MITOCHONDRIAL CYTOCHROME C RELEASE VIA MEMBRANE PORES
    Elliott Crouser; Fiscal Year: 2004
    ..These advisors are actively investigating the role mitochondria in the pathogenesis of disease. Thus, the prospects for successful collaborative initiatives, a critical determinant of future success, are excellent. ..
  32. MMPs and Synaptic Injury with HIV/METH
    Katherine Conant; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  33. Novel Therapies for Metabolic Complications in Patients with Lipodystrophies
    Abhimanyu Garg; Fiscal Year: 2010
    ..Additionally, the findings may be applicable to treating metabolic complications in patients with obesity and type 2 diabetes. ..
  34. MOUSE MODELS TO STUDY MULIBREY NANISM
    Juan Zapata; Fiscal Year: 2005
    ..study the developmental and phenotypic alterations of trim37 knock out and FINmajor-mutated trim37 mice. These mice will be the foundation for further studies on the biology and etiology of Mulibrey Nanism. ..
  35. DIET, IMMUNE MODULATION, AND ASTHMA IN EARLY LIFE
    Scott Weiss; Fiscal Year: 2002
    ....
  36. Vasoactive Peptides that Stimulate Smooth Muscles
    RANDAL A contact SKIDGEL; Fiscal Year: 2010
    ..abstract_text> ..
  37. 2006 Clinical Research Training Institute
    James George; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  38. Molecular and Cellular Characterization of Myeloproliferative Disease
    KATHLEEN MIHO SAKAMOTO; Fiscal Year: 2010
    ..These studies will provide new insights into the molecular pathways leading to MPD. ..
  39. GENE REGULATION DURING INTERMITTENT HYPOXIA
    Nanduri Prabhakar; Fiscal Year: 2003
    ....
  40. SIGNAL TRANSDUCTION FROM THE T CELL ANTIGEN RECEPTOR
    Robert Abraham; Fiscal Year: 2003
    ..However, experiments in fibroblasts stimulated with PDGF indicate that phosphorylation at residue 783 is necessary for increased enzymatic activity, while phosphorylation at Y771 results in inhibition of activity. ..
  41. MECHANISMS OF LIPODYSTROPHY IN HIV INFECTED PATIENTS
    Abhimanyu Garg; Fiscal Year: 2002
    ..Additionally, the study may prove effectiveness and reduced toxicity of alternative antiretroviral regimens without a PI. ..
  42. EPITHELIAL/MESENCHYMAL INTERACTIONS IN LUNG DEVELOPMENT
    Farrah Kheradmand; Fiscal Year: 2002
    ..Michael Mafthay, Carol Basbaum, and Gerald Cunha, all senior faculty members of Division of Pulmonary and Department of Anatomy at UCSF. (End of Abstract) ..
  43. ERM and Rho Signal Pathways in T Cell Immune Senescence
    GONZALO GARCIA; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  44. Matrix metalloproteinases as biomarkers of neurodegeneration
    Veronica Shubayev; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  45. Thrombin signaling and HIV dementia
    Katherine Conant; Fiscal Year: 2006
    ..abstract_text> ..
  46. Oxidative Stress And Sympathetic Nerve Activity
    Vito Campese; Fiscal Year: 2006
    ..If our hypothesis were to be correct, administration of inhibitors of the renin-angiotensin system particularly if combined with antioxidants should result in better control of BP in these models. ..
  47. Analysis of Toxin A synthesis in P. aeruginosa
    Abdul Hamood; Fiscal Year: 2006
    ....
  48. STRUCTURE AND FUNCTION OF THE COP9 SIGNALOSOME
    Ning Wei; Fiscal Year: 2005
    ....
  49. INHIBITION OF THE ANGIOGENIC RESPONSE
    Luisa Iruela Arispe; Fiscal Year: 2006
    ..abstract_text> ..
  50. The Role of Proteinases in Experimental Asthma
    Farrah Kheradmand; Fiscal Year: 2006
    ..Together these findings will substantially contribute to the understanding of regulation of Th2 immunity and will provide new approaches for the treatment of asthma. [unreadable] [unreadable] [unreadable]..
  51. DUX4 and Facioscapulohumeral Muscular Dystrophy
    Howard Worman; Fiscal Year: 2004
    ..This work could lead to the development of new diagnostic methods as well as the identification of potential protein targets for the treatment of FSHD. ..
  52. CHOLINE ACETYLTRANSFERASE
    Louis Hersh; Fiscal Year: 2004
    ....
  53. Antiidiotype mAb:an Antiangiogenic /Antimetastatic Agent
    FRANCIS MARKLAND; Fiscal Year: 2002
    ..According to the Angiogenesis Foundation, diseases that may be treatable with angiogenesis-based drugs encompass markets representing 20% of the $322 billion global pharmaceutical market. ..
  54. Lamin A Mutation and Hutchinson-Gilford Progeria
    Howard Worman; Fiscal Year: 2008
    ..This project will establish how mutations in nuclear lamins A and C cause HGPS, and if inhibition of protein farnesylation is a potential therapeutic intervention. ..
  55. Estrogen, androgen, neprilysin, and amyloid peptides.
    Louis Hersh; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  56. Second International Workshop on Microsporidia from Vertebrate and Invertebrate H
    Louis Weiss; Fiscal Year: 2007
    ..Overall, the goal of this[unreadable] congress is to foster the exchange of information between these diverse groups of[unreadable] scientists leading to advances in research on these emergent pathogens. ..
  57. Liposomal Disintegrin: Novel and Effective Antitumor Agent Phase I
    FRANCIS MARKLAND; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. International Network and Registry for TMA
    Howard Trachtman; Fiscal Year: 2007
    ..These include essential hypertension, diabetes, hyperlipidemia, myocardial infarction, and chronic kidney disease. [unreadable] [unreadable]..
  59. Lamin A in Adipocyte Differentation and Survival
    Howard Worman; Fiscal Year: 2004
    ..Confirmation of this hypothesis will have implications for the identification of new cellular targets to treat human obesity and diabetes mellitus. ..
  60. GROWTH FACTORS IN THE ADULT AND AGING BRAIN
    James Roberts; Fiscal Year: 2004
    ..Thus, we propose to establish whether new dopamine neurons are produced in response to injury or can be stimulated to differentiate by ectopically expressing cytokines, which are able to regulate neuronal. ..
  61. Induction of Inflammation by Mitochondrial Proteins
    Elliott Crouser; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  62. ROLE OF MYOSTATIN IN CACHEXIA
    Se Jin Lee; Fiscal Year: 2005
    ..abstract_text> ..