beta galactosidase


Summary: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS GM1.

Top Publications

  1. ncbi Three-dimensional structure of beta-galactosidase from E. coli
    R H Jacobson
    Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene 97403
    Nature 369:761-6. 1994
  2. ncbi Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells
    D J Kurz
    Cell Biology Group, Centre for Cardiovascular Biology and Medicine, Department of Medicine, Royal Free and University College Medical School, University College London, London WC1E 6JJ, UK
    J Cell Sci 113:3613-22. 2000
  3. ncbi Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells
    H Ota
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Oncogene 25:176-85. 2006
  4. pmc SIRT1 overexpression antagonizes cellular senescence with activated ERK/S6k1 signaling in human diploid fibroblasts
    Jing Huang
    Peking University Research Center on Aging, Beijing, People s Republic of China
    PLoS ONE 3:e1710. 2008
  5. ncbi Senescence-associated beta-galactosidase is lysosomal beta-galactosidase
    Bo Yun Lee
    Department of Life Science, University of Seoul, Dongdaemungu, Jeonnongdong, Seoul, Korea 130 743
    Aging Cell 5:187-95. 2006
  6. ncbi Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
  7. ncbi Extension of life-span by introduction of telomerase into normal human cells
    A G Bodnar
    Geron Corporation, Menlo Park, CA 94025, USA
    Science 279:349-52. 1998
  8. ncbi Sirt1 modulates premature senescence-like phenotype in human endothelial cells
    Hidetaka Ota
    Department of Geriatric Medicine, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    J Mol Cell Cardiol 43:571-9. 2007
  9. ncbi A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
    Melanie Jonuscheit
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
    Mol Microbiol 48:1241-52. 2003
  10. pmc T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification
    T P Yamaguchi
    Department of Molecular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:3185-90. 1999

Research Grants

Detail Information

Publications238 found, 100 shown here

  1. ncbi Three-dimensional structure of beta-galactosidase from E. coli
    R H Jacobson
    Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene 97403
    Nature 369:761-6. 1994
    ..Non-crystallographic symmetry averaging proved to be a very powerful tool in the structure determination, as has been shown in other contexts...
  2. ncbi Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells
    D J Kurz
    Cell Biology Group, Centre for Cardiovascular Biology and Medicine, Department of Medicine, Royal Free and University College Medical School, University College London, London WC1E 6JJ, UK
    J Cell Sci 113:3613-22. 2000
    ..These findings demonstrate that senescence-associated (beta)-galactosidase is a manifestation of residual lysosomal activity at a suboptimal pH, which becomes detectable due to the increased lysosomal content in senescent cells...
  3. ncbi Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells
    H Ota
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Oncogene 25:176-85. 2006
    ..These results suggest that inhibitors for Sirt1 may have anticancer potential, and that impaired activation of Ras-MAPK pathway might take part in a senescence-like growth arrest program induced by Sirtinol...
  4. pmc SIRT1 overexpression antagonizes cellular senescence with activated ERK/S6k1 signaling in human diploid fibroblasts
    Jing Huang
    Peking University Research Center on Aging, Beijing, People s Republic of China
    PLoS ONE 3:e1710. 2008
    ..These findings suggested that SIRT1-promoted cell proliferation and antagonized cellular senescence in human diploid fibroblasts may be, in part, via the activation of ERK/ S6K1 signaling...
  5. ncbi Senescence-associated beta-galactosidase is lysosomal beta-galactosidase
    Bo Yun Lee
    Department of Life Science, University of Seoul, Dongdaemungu, Jeonnongdong, Seoul, Korea 130 743
    Aging Cell 5:187-95. 2006
    ..SA-beta-gal induction during senescence was due at least in part to increased expression of the lysosomal beta-galactosidase protein. These results also indicate that SA-beta-gal is not required for senescence...
  6. ncbi Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
    ..This microfluidic single cell assay opens up possibilities for system-wide characterization of the expression of these low copy number proteins...
  7. ncbi Extension of life-span by introduction of telomerase into normal human cells
    A G Bodnar
    Geron Corporation, Menlo Park, CA 94025, USA
    Science 279:349-52. 1998
    ..The ability to maintain normal human cells in a phenotypically youthful state could have important applications in research and medicine...
  8. ncbi Sirt1 modulates premature senescence-like phenotype in human endothelial cells
    Hidetaka Ota
    Department of Geriatric Medicine, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    J Mol Cell Cardiol 43:571-9. 2007
    ..Our data suggest that Sirt1 may exert protective effects against endothelial dysfunction by preventing stress-induced premature senescence and deranged expression of PAI-1 and eNOS...
  9. ncbi A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
    Melanie Jonuscheit
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
    Mol Microbiol 48:1241-52. 2003
  10. pmc T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification
    T P Yamaguchi
    Department of Molecular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:3185-90. 1999
    ..Our results suggest that Wnt3a, signaling via Brachyury, modulates a balance between mesodermal and neural cell fates during gastrulation...
  11. pmc Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells
    B P Zambrowicz
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 94:3789-94. 1997
    ..This third transcript potentially encodes a novel protein of at least 505 amino acids that is conserved in humans and in Caenorhabditis elegans...
  12. ncbi Expression of beta-galactosidase and beta-xylosidase genes during microspore and pollen development
    Petra Hrubá
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Pernikarce 15, 160 00, Prague 6, Czech Republic
    Planta 220:931-40. 2005
  13. pmc FlhA influences Bacillus thuringiensis PlcR-regulated gene transcription, protein production, and virulence
    Laurent Bouillaut
    Unité Génétique Microbienne et Environnement, INRA, La Minière, 78285 Guyancourt Cedex, France
    Appl Environ Microbiol 71:8903-10. 2005
    ..The decrease in virulence may be due to both a lack of flagella and a lower production of secreted factors. Hence, FlhA appears to be an essential virulence factor with a pleiotropic role...
  14. ncbi Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice
    R Kothary
    Division of Molecular and Developmental Biology, Mt Sinai Hospital Research Institute, Toronto, Ontario, Canada
    Development 105:707-14. 1989
    ..This tightly regulated inducible promoter may provide a useful tool for short-term inducible gene expression in transgenic mice...
  15. ncbi Photoacoustic imaging of lacZ gene expression in vivo
    Li Li
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 12:020504. 2007
    ..With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research...
  16. pmc High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation
    D H Juers
    Institute of Molecular Biology, and Department of Physics, University of Oregon, Eugene 97403 1229, USA
    Protein Sci 9:1685-99. 2000
    ..Taken together, the overall arrangement provides a structural basis for the phenomenon of alpha-complementation...
  17. ncbi Neuroprotective effect of a CNTF-expressing lentiviral vector in the quinolinic acid rat model of Huntington's disease
    L P de Almeida
    Division of Surgical Research and Gene Therapy Center, Lausanne Medical School, Switzerland
    Neurobiol Dis 8:433-46. 2001
    ..These results further establish that lentiviruses may represent an efficient gene delivery system for the screening of therapeutic molecules in Huntington's disease...
  18. ncbi Loss of AP-2alpha impacts multiple aspects of ventral body wall development and closure
    Stephanie Brewer
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Biol 267:399-417. 2004
    ..These findings provide a fundamental understanding of how AP-2alpha functions in the closure of the ventral body wall, as well as offer insight into related human birth defects...
  19. ncbi Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus
    Qian Zhang
    Genelux Corporation, San Diego Science Center, San Diego, California 92109, USA
    Cancer Res 67:10038-46. 2007
    ..These findings suggest that immune activation may combine with viral oncolysis to induce tumor eradication in this model, providing a novel perspective for the design of oncolytic viral therapies for human cancers...
  20. pmc Immunotherapy with dendritic cells directed against tumor antigens shared with normal host cells results in severe autoimmune disease
    B Ludewig
    Institute of Experimental Immunology, CH 8091 Zurich, Switzerland
    J Exp Med 191:795-804. 2000
    ..These results reveal the delicate balance between tumor immunity and autoimmunity and therefore point out important limitations for the use of not strictly tumor-specific antigens in antitumor vaccination with DCs...
  21. ncbi The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase
    N Illing
    Sir William Dunn School of Pathology, University of Oxford, UK
    Mol Microbiol 5:1927-40. 1991
    ..Since the onset of spoIIID expression occurs about 10 minutes after that of spoIIIA, spoIIIA expression is transient. Thus spoIIIA defines a third temporal class of gene controlled by the sigma E form of RNA polymerase...
  22. ncbi Oligopeptide permease is required for expression of the Bacillus thuringiensis plcR regulon and for virulence
    M Gominet
    , CNRS (URA2172, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex, France
    Mol Microbiol 40:963-75. 2001
    ..subtilis. However, analysis of plcR expression in DeltaoppB, Deltaspo0A and DeltaoppB Deltaspo0A mutants indicates that Opp is required for plcR expression via a Spo0A-independent mechanism...
  23. ncbi Synchronous activity of inhibitory networks in neocortex requires electrical synapses containing connexin36
    M R Deans
    Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Neuron 31:477-85. 2001
    ..However, the synchrony of these rhythms was weaker and more spatially restricted in the knockout. We conclude that electrical synapses containing Cx36 are critical for the generation of widespread, synchronous inhibitory activity...
  24. pmc Ink4a/Arf expression is a biomarker of aging
    Janakiraman Krishnamurthy
    Department of Medicine, The Lineberger Comprehensive Cancer Center, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27599 7295, USA
    J Clin Invest 114:1299-307. 2004
    ..These data suggest that expression of the Ink4a/Arf tumor suppressor locus is a robust biomarker, and possible effector, of mammalian aging...
  25. pmc Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes
    B Hartl
    Institute of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany
    J Bacteriol 183:2696-9. 2001
    ..These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes...
  26. ncbi Senescence delay of human diploid fibroblast induced by anti-sense p16INK4a expression
    J Duan
    Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, Beijing 100083, People's Republic of China
    J Biol Chem 276:48325-31. 2001
    ..These data not only strongly support a role for p16(INK4a) in replicative senescence but also raise the possibility of using the antisense p16(INK4a) therapeutically...
  27. ncbi Microfabricated arrays of femtoliter chambers allow single molecule enzymology
    Yannick Rondelez
    Nat Biotechnol 23:361-5. 2005
    ..The approach should be of interest for many ultrasensitive bioassays at the single-molecule level...
  28. ncbi Cold-active beta-galactosidase from Arthrobacter sp. C2-2 forms compact 660 kDa hexamers: crystal structure at 1.9A resolution
    Tereza Skalova
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho nam 2, 1606 Praha 6, Czech Republic
    J Mol Biol 353:282-94. 2005
    ..This structure reveals new insights into the cold-adaptation mechanisms of enzymatic pathways of extremophiles...
  29. pmc Identification of new flagellar genes of Salmonella enterica serovar Typhimurium
    Jonathan Frye
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Bacteriol 188:2233-43. 2006
    ..Null alleles in cheV, mcpA, mcpB, mcpC, and srfB did not affect motility, while deletion of yhjH did result in reduced motility compared to that of the wild type...
  30. pmc Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes
    Isabelle Ventre
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, Unite Propre de Recherche 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 103:171-6. 2006
  31. ncbi Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose
    A L Rojas
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Av Trabalhador Sao Carlense 400, CEP 13560 970 São Carlos, SP, Brazil
    J Mol Biol 343:1281-92. 2004
    ..Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date...
  32. pmc Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli
    H M Giaever
    Institute of Fisheries, University of Tromsø, Norway
    J Bacteriol 170:2841-9. 1988
    ..But an osmotically tolerant phenotype was restored in the presence of glycine betaine, which also partially repressed the synthesis of synthase in the wild type and of beta-galactosidase in ots-lacZ fusion mutants...
  33. pmc Vascular bed-specific expression of an endothelial cell gene is programmed by the tissue microenvironment
    W C Aird
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02119, USA
    J Cell Biol 138:1117-24. 1997
    ..Taken together, the results suggest that the vWF gene is regulated by vascular bed-specific pathways in response to signals derived from the local microenvironment...
  34. ncbi Temperature alternation by an on-chip microheater to reveal enzymatic activity of beta-galactosidase at high temperatures
    Hideyuki F Arata
    Institute of Industrial Science, The University of Tokyo, Meguro ku, Tokyo 153 8505, Japan
    Anal Chem 77:4810-4. 2005
    ..Its activity at 60 degrees C was revealed to be approximately 4 times greater than that at room temperature. This method not only gives new kinetic information in biochemistry but also enables application in highly sensitive biosensors...
  35. pmc Molecular cloning of a {beta}-galactosidase from radish that specifically hydrolyzes {beta}-(1->3)- and {beta}-(1->6)-galactosyl residues of Arabinogalactan protein
    Toshihisa Kotake
    Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Sakura ku, Saitama 338 8570, Japan
    Plant Physiol 138:1563-76. 2005
  36. ncbi Irreversible cellular senescence induced by prolonged exposure to H2O2 involves DNA-damage-and-repair genes and telomere shortening
    Jianming Duan
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China
    Int J Biochem Cell Biol 37:1407-20. 2005
    ..Our findings indicate that several aging theories can be merged together by a common mechanism of oxidative damage, and that the level of oxidative DNA-damage-and-repair capacity may be exploited as reliable markers of cell senescence...
  37. pmc Cold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis
    A Hoyoux
    Laboratory of Biochemistry, Institute of Chemistry, University of Liege, Sart-Tilman, B-4000 Liege, Belgium
    Appl Environ Microbiol 67:1529-35. 2001
    ..lactis, suggesting that the cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants...
  38. pmc Senescence associated beta galactosidase activity in human retinal pigment epithelial cells exposed to mild hyperoxia in vitro
    Shigeru Honda
    Department of Ophthalmology, University of California, Davis, CA, USA
    Br J Ophthalmol 86:159-62. 2002
    ..To determine if mild hyperoxia induces senescence in retinal pigment epithelium (RPE) cells in vitro...
  39. ncbi Beta galactosidase enzyme fragment complementation as a novel technology for high throughput screening
    Richard M Eglen
    Research and Development, DiscoveRx Corp, 42501 Albrae St, Suite 100, Fremont, CA 94538, USA
    Comb Chem High Throughput Screen 6:381-7. 2003
    In this review, the applications of beta galactosidase complementation are described. alpha Complementation is a naturally occurring process in bacteria and in engineered cells, and can also occur in eukaryotic cells...
  40. ncbi kappa B-like motifs regulate the induction of immune genes in Drosophila
    Y Engstrom
    Department of Molecular Biology, Stockholm University, Sweden
    J Mol Biol 232:327-33. 1993
    ..Our data suggest a functional and evolutionary relationship between these insect immune response factors and the mammalian NF-kappa B...
  41. ncbi Thermal denaturation of beta-galactosidase and of two site-specific mutants
    R A Edwards
    Division of Biochemistry, Faculty of Science, University of Calgary, Alberta, Canada
    Biochemistry 29:11001-8. 1990
    ..Other effects result from changes in ligand binding, but the magnitude of the resulting changes in stability is not related to the strength of ligand binding in a simple fashion...
  42. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  43. ncbi Analyzing mRNA-protein complexes using a yeast three-hybrid system
    David S Bernstein
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Methods 26:123-41. 2002
    ..Multicomponent complexes containing more than one protein can be detected, identified, and analyzed. We describe the method and how to use it, and discuss applications that bear particularly on eukaryotic mRNAs...
  44. ncbi Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice
    G Friedrich
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
    Genes Dev 5:1513-23. 1991
    ..Because no overt phenotype was detected in the remaining strains, these results suggest that a substantial proportion of mammalian genes identified by this approach are not essential for development...
  45. ncbi A selectable bifunctional beta-galactosidase::phleomycin-resistance fusion protein as a potential marker for eukaryotic cells
    M Baron
    Laboratoire de Microbiologie, Universite Paul Sabatier, Toulouse, France
    Gene 114:239-43. 1992
    ..This system appears to be a potentially useful tool for the direct selection of transformants, and the evaluation of gene expression and regulation in a wide variety of prokaryotic and eukaryotic hosts...
  46. ncbi Endothelial cell senescence in human atherosclerosis: role of telomere in endothelial dysfunction
    Tohru Minamino
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan
    Circulation 105:1541-4. 2002
    ..However, whether senescent vascular cells are present in the vasculature and contribute to the pathogenesis of atherosclerosis remains unclear...
  47. ncbi Papaya beta-galactosidase/galactanase isoforms in differential cell wall hydrolysis and fruit softening during ripening
    Hamid Lazan
    Faculty of Science and Technology, School of Biosciences and Biotechnology, University Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia
    Plant Physiol Biochem 42:847-53. 2004
    ..Collectively, the results support the case for a functional relevance of the papaya enzymes in softening related changes during ripening...
  48. pmc A high-throughput screen for synthetic riboswitches reveals mechanistic insights into their function
    Sean A Lynch
    Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, GA 30322, USA
    Chem Biol 14:173-84. 2007
    ..We anticipate that the screening method and design principles will be generally useful for creating functional synthetic riboswitches...
  49. pmc Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration
    Christine Lyn Le Maitre
    Tissue Injury and Repair Group, School of Medicine, Stopford Building, The University of Manchester, Oxford Road, Manchester, UK, M13 9PT
    Arthritis Res Ther 9:R45. 2007
    ..Furthermore, the senescent phenotype is associated with increased catabolism, implicating cellular senescence in the pathogenesis of IVD degeneration...
  50. pmc Expression of senescence-related genes in human corneal endothelial cells
    Zhenhua Song
    State Key Lab Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
    Mol Vis 14:161-70. 2008
    ..To investigate the expression of p16(INK4a), p21(WAF1/CIP1), p27(KIP1), and p53 in human corneal endothelial cell (HCEC) senescence ex vivo at various donor ages...
  51. ncbi Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35
    Young Ock Ahn
    Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Phytochemistry 68:1510-20. 2007
    ..It also cleaved beta-(1,3)-, beta-(1,4)-, and beta-(1,6)-linked galactobiosides and galactotriosides, showing a marked preference for beta-(1,3)- and beta-(1,4)-linkages...
  52. ncbi A structural view of the action of Escherichia coli (lacZ) beta-galactosidase
    D H Juers
    Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Biochemistry 40:14781-94. 2001
    ..The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation...
  53. ncbi Multimeric connexin interactions prior to the trans-Golgi network
    J Das Sarma
    Institute for Environmental Medicine, Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Cell Sci 114:4013-24. 2001
    ..In contrast, ROS osteoblastic cells, which differentially sort Cx43 and Cx46, did not form Cx43/Cx46 heteromers. Thus, cells have the capacity to regulate whether or not compatible connexins intermix...
  54. ncbi Inducible Cre-mediated control of gene expression in the murine gastrointestinal tract: effect of loss of beta-catenin
    Heather Ireland
    Department of Oncology, Cancer Research UK, Cambridge Institute of Medical Research, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Gastroenterology 126:1236-46. 2004
  55. ncbi Mode of action of the TyrR protein: repression and activation of the tyrP promoter of Escherichia coli
    Ji Yang
    Department of Microbiology and Immunology, The University of Melbourne, Victoria, 3010, Australia
    Mol Microbiol 52:243-56. 2004
    ..This observation coupled with the results from mutational analysis supports the proposal that TyrR-phenylalanine activates tyrP transcription by stimulating the onset of open complex formation...
  56. ncbi Antisense suppression of a beta-galactosidase gene (TB G6) in tomato increases fruit cracking
    Edgar Moctezuma
    Produce Quality and Safety Laboratory, USDA ARS, Building 002, Henry A Wallace Beltsville Area Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    J Exp Bot 54:2025-33. 2003
    ..Although the exact function of the TBG6 product is still unknown, these results implicate an important role for this enzyme in early fruit growth and development in tomato...
  57. pmc Detailed map of a cis-regulatory input function
    Y Setty
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 100:7702-7. 2003
    ..This possibility means that the wild-type region is selected to perform an elaborate computation in setting the transcription rate. The present approach can be generally used to map the input functions of other genes...
  58. pmc The function of SECIS RNA in translational control of gene expression in Escherichia coli
    Martin Thanbichler
    Department of Biology I, Microbiology, University of Munich, Maria Ward Strasse 1a, D 80638 Munich, Germany
    EMBO J 21:6925-34. 2002
  59. ncbi Central role of the proteasome in senescence and survival of human fibroblasts: induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation
    Niki Chondrogianni
    National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, 48 Vas Constantinou Ave, Athens 11635, Greece
    J Biol Chem 278:28026-37. 2003
    ..In summary these data demonstrate the central role of the proteasome during cellular senescence and survival as well as provide insights toward a better understanding of proteasome regulation...
  60. pmc The antibacterial arm of the drosophila innate immune response requires an IkappaB kinase
    Y Lu
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Genes Dev 15:104-10. 2001
    ..The ird5 gene encodes a Drosophila homolog of mammalian IkappaB kinases (IKKs). The ird5 phenotype and sequence suggest that the gene is specifically required for the activation of Relish, a Drosophila NF-kappaB family member...
  61. pmc Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes
    G F Chen
    Department of Biochemistry, Robert Wood Johnson Medical School UMDNJ, Rutgers, Piscataway 08854
    Nucleic Acids Res 18:1465-73. 1990
    ..From these results, we propose that the position of the minor codons in an mRNA plays an important role in the regulation of gene expression possibly by modulating the stability of the initiation complex for protein synthesis...
  62. pmc Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice
    X Mao
    Division of Hematology Oncology, Department of Pediatrics, Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:5037-42. 1999
    ..This improved reporter strain should facilitate monitoring in vivo Cre-mediated excision events in a variety of experimental contexts...
  63. ncbi Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli
    J C Gebler
    Department of Chemistry, University of British Columbia, Vancouver, Canada
    J Biol Chem 267:11126-30. 1992
    ..and Legler, G. (1984) Eur. J. Biochem. 138, 527-531) on the basis of affinity labeling studies with conduritol C cis-epoxide. A role for glutamic acid residue 461 as the acid/base catalyst is proposed and justified...
  64. ncbi Insulin-like growth factor binding protein-related protein 1 inhibits proliferation of MCF-7 breast cancer cells via a senescence-like mechanism
    Heather Marie P Wilson
    Department of Pathology, University of Washington School of Medicine, Seattle 98195, USA
    Cell Growth Differ 13:205-13. 2002
    ..These findings indicate that IGFBP-rP1 is an inhibitor of MCF-7 breast cancer cell proliferation and may act via a cellular senescence-like mechanism...
  65. ncbi Treatment of anaplastic thyroid carcinoma in vitro with a mutant vaccinia virus
    Shu Fu Lin
    Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Surgery 142:976-83; discussion 976-83. 2007
    ..Anaplastic thyroid carcinoma (ATC) is a fatal disease resistant to all conventional treatments. Novel therapies are needed to improve dismal outcomes...
  66. ncbi Hair follicle renewal: organization of stem cells in the matrix and the role of stereotyped lineages and behaviors
    Emilie Legue
    Unité de Biologie moléculaire du Développement, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Development 132:4143-54. 2005
    ..Thus, our studies suggest that fate and behavior are organized by two systems (uncoupled), and this uncoupling may represent a fundamental way to simplify morphogenesis...
  67. ncbi Telomere erosion and senescence in human articular cartilage chondrocytes
    J A Martin
    Department of Orthopaedic Surgery, Iowa City Veterans Administration Medical Center and University of Iowa, Iowa City 52242, USA
    J Gerontol A Biol Sci Med Sci 56:B172-9. 2001
    ..The data also imply that transplantation procedures performed to restore damaged articular surfaces could be limited by the inability of older chondrocytes to form new cartilage after transplantation...
  68. ncbi Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans
    Allen L Honeyman
    Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, 12901 Bruce B Downs Boulevard, Tampa, FL 33612, USA
    J Microbiol Methods 49:163-71. 2002
    ..mutans. The plasmids described in this paper will provide new tools and techniques for the analysis of S. mutans gene regulation. In addition, we have compiled the complete nucleotide sequences of these gene reporter plasmids...
  69. ncbi Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesis
    C G Cupples
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Biol Chem 265:5512-8. 1990
    ..The enzyme with His substituted for Glu-461 had the highest kappa 3 value. This is probably a result of the formation of a covalent bond between His and the galactosyl part of the substrate...
  70. pmc Caspase-mediated processing of the Drosophila NF-kappaB factor Relish
    Svenja Stoven
    Umea Centre for Molecular Pathogenesis, Umea University, S 901 87 Umea, Sweden
    Proc Natl Acad Sci U S A 100:5991-6. 2003
    ..Finally, an N-terminal serine-rich region in Relish and the PEST domain were found to negatively regulate Relish activation...
  71. ncbi Efficient retroviral gene transfer to the liver in vivo using nonpolypeptidic mitogens
    V Pichard
    , , Centre Hospitalier Universitaire de Nantes, Nantes Cedex 01, 44035, France
    Biochem Biophys Res Commun 286:929-35. 2001
    ..Since these two compounds have a long history of safe clinical use, we propose that these liver mitogens may have potential for clinical application in liver-directed gene therapy...
  72. ncbi Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability
    Hoang Duc Nguyen
    Institute of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany Vietnam National University Ho Chi Minh City, College of Natural Sciences, Faculty of Biology, Ho Chi Minh City, Viet Nam
    Plasmid 54:241-8. 2005
    ..The versatility of these expression vectors was demonstrated by fusing their promoters to a reporter gene and by overexpression of the HtpG protein with three of them. All recombinant vectors exhibited full structural stability...
  73. pmc Senescence in human intervertebral discs
    S Roberts
    Centre for Spinal Studies, Robert Jones and Agnes Hunt Orthopaedic and District Hospital, NHS Trust, Oswestry, Shropshire, SY10 7AG, UK
    Eur Spine J 15:S312-6. 2006
  74. pmc Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an Antarctic arthrobacter isolate
    James A Coker
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 185:5473-82. 2003
    ..Mutation studies of the BgaS enzyme should aid our understanding of such specific, localized changes affecting enzyme thermal properties...
  75. ncbi Lactose utilization by Saccharomyces cerevisiae strains expressing Kluyveromyces lactis LAC genes
    M Rubio-Texeira
    Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Cientificas, Apartado de Correos 73, E46100, Valencia, Burjassot, Spain
    J Biotechnol 84:97-106. 2001
    ..Additionally, we describe the construction of a new strain, which combines the Lac(+) phenotype with additional properties of biotechnological interest: flocculence, and the ability to hydrolyze starch...
  76. pmc Phosphorylation near nuclear localization signal regulates nuclear import of adenomatous polyposis coli protein
    F Zhang
    Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Building 555, Room 5263, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 97:12577-82. 2000
    ..Our data provide evidence that control of APC's nuclear import through phosphorylation is a potential mechanism for regulating APC's nuclear activity...
  77. ncbi Cytotoxic immune response blunts long-term transgene expression after efficient retroviral-mediated hepatic gene transfer in rat
    Dominique Aubert
    Laboratoire de Thérapie Génique INSERM 0105, Nantes, France
    Mol Ther 5:388-96. 2002
    ..This study demonstrates that retrovirus-mediated gene transfer may induce cytotoxic T lymphocytes specifically directed against transgene-expressing cells...
  78. ncbi Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media
    Manuel Becerra
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    J Biotechnol 109:131-7. 2004
    ..This strain is effective for the heterologous production and release of K. lactis beta-galactosidase into the extra-cellular medium after osmotic shock...
  79. ncbi Differential effects of genotoxic stress on both concurrent body growth and gradual senescence in the adult zebrafish
    Stephanie B Tsai
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, USA
    Aging Cell 6:209-24. 2007
    ..Moreover, our present findings indicate that genotoxic DNA damage may play a role as a rate-limiting factor during the induction of senescence, but not in the inhibition of continuous, density-dependent growth in adult zebrafish...
  80. ncbi Markers of senescence?
    Philip J Coates
    J Pathol 196:371-3. 2002
  81. ncbi Eukaryotic promoters can direct protein synthesis in Gram-negative bacteria
    Sylvie Goussard
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Mol Microbiol Biotechnol 6:211-8. 2003
    ..These observations should incite to exert caution when interpreting data on the DNA transfer from bacteria to mammallian cells when the genes of interest are under the control of the PCMV or PRSV promoter...
  82. ncbi In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates
    I Matsumura
    Institute of Cellular and Molecular Biology, ICMB A4800 MBB 3 424, University of Texas, 26th and Speedway, Austin, TX, 78712, USA
    J Mol Biol 305:331-9. 2001
    ..These results are consistent with the "patchwork" hypothesis, which postulates that modern enzymes diverged from ancestors with broad specificity...
  83. ncbi In vivo imaging of beta-galactosidase activity using far red fluorescent switch
    Ching Hsuan Tung
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Cancer Res 64:1579-83. 2004
    ..The results indicated that in vivo real-time detection of beta-gal activity is possible by fluorescence imaging technology...
  84. pmc Novel oncolytic agent GLV-1h68 is effective against malignant pleural mesothelioma
    Kaitlyn J Kelly
    Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Hum Gene Ther 19:774-82. 2008
    ..GLV-1h68 was successfully used to treat MPM in vitro and in an orthotopic model (in vivo). These promising results warrant clinical investigation of GLV-1h68 as a novel agent in the treatment of MPM...
  85. ncbi Regulation of cellular senescence and p16(INK4a) expression by Id1 and E47 proteins in human diploid fibroblast
    Wenjie Zheng
    Department of Biochemistry and Molecular Biology, Peking University, Health Science Center, 38 Xueyuan Road, Beijing 100083, People s Republic of China
    J Biol Chem 279:31524-32. 2004
  86. ncbi Endless versatility in the biotechnological applications of Kluyveromyces LAC genes
    Marta Rubio-Texeira
    68 541, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, USA
    Biotechnol Adv 24:212-25. 2006
    ..Emphasis is also made on the potential that some of the latest technologies, such as the generation of transgenics, will have for a further benefit in the use of these and related genes...
  87. ncbi Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
    Thomas S Wehrman
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Methods 3:295-301. 2006
    ..Thus, coupling the properties of FLuc to the advantages of beta-gal permits bioluminescent imaging applications that previously were not possible...
  88. ncbi Purification and characterization of a beta-galactosidase from peach (Prunus persica)
    Dong Hoon Lee
    Department of Horticulture, Yeungnam University, Gyeongsan 712 749, Korea
    Mol Cells 15:68-74. 2003
    ..Therefore, enzymatic, immunological, and molecular evidences in this study indicate that the purified 42-kDa protein is a peach beta-galactosidase...
  89. ncbi Novel NMR approach to assessing gene transfection: 4-fluoro-2-nitrophenyl-beta-D-galactopyranoside as a prototype reporter molecule for beta-galactosidase
    Weina Cui
    Department of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9058, USA
    Magn Reson Med 51:616-20. 2004
    ..Molecular and 19F NMR characteristics of PFONPG in solution, blood, and prostate tumor cells are presented. This prototype molecule facilitates a novel approach for assaying gene activity in vivo...
  90. ncbi Fluctuations in transcription factor binding can explain the graded and binary responses observed in inducible gene expression
    Jason R Pirone
    Biomathematics Graduate Program and Department of Environmental and Molecular Toxicology, Campus Box 8203, North Carolina State University, Raleigh, NC 27695 8203, USA
    J Theor Biol 226:111-21. 2004
    ..Our modeling results are in good qualitative agreement with the experimental data, and illustrate how the binary and graded responses can stem from the same underlying mechanism...
  91. ncbi Cloning of a Cicer arietinum beta-galactosidase with pectin-degrading function
    Rocío Esteban
    Dpto de Fisiología Vegetal Facultad de Biología, Universidad de Salamanca, Centro Hispano Luso de Investigaciones Agrarias, Pza Doctores de la Reina s n, Salamanca 37007, Spain
    Plant Cell Physiol 44:718-25. 2003
    ..It is suggested that the increase in the size of cell wall pores could permit access of other cell wall-modifying enzymes to their substrate...
  92. ncbi Superoxide dismutase 1 knock-down induces senescence in human fibroblasts
    Gil Blander
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 278:38966-9. 2003
    ..Together, these findings support the notion that in normal human cells the SOD1 protein may play a role in the regulation of cellular lifespan by p53 and may also regulate the death signals in cancer cells...
  93. ncbi Mature hepatocytes are the source of small hepatocyte-like progenitor cells in the retrorsine model of liver injury
    Audrey Avril
    Biothérapies Hépatiques, CIC INSERM 00 04, CHU Hotel Dieu, 44093 Nantes Cedex 01, France
    J Hepatol 41:737-43. 2004
    ..Our aim was to test whether SHPCs could originate from mature hepatocytes...
  94. ncbi The zebrafish as a vertebrate model of functional aging and very gradual senescence
    Shuji Kishi
    Department of Cancer Biology, Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, MA 02115 6084, USA
    Exp Gerontol 38:777-86. 2003
  95. ncbi Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
    Hubert Cieśliński
    Department of Microbiology, Gdansk University of Technology, ul G Narutowicza 11 12, 80 952 Gdansk, Poland
    Protein Expr Purif 39:27-34. 2005
    ..Because of its attributes, the recombinant Pseudoalteromonas sp. 22b beta-galactosidase could be applied at refrigeration temperatures for production of lactose-reduced dairy products...
  96. pmc Transcriptional regulation of sitABCD of Salmonella enterica serovar Typhimurium by MntR and Fur
    Jack S Ikeda
    Department of Microbiology, University of Illinois, B103 CLSL, 601 S Goodwin Ave, Urbana, IL 61801, USA
    J Bacteriol 187:912-22. 2005
  97. ncbi The role of chondrocyte senescence in the pathogenesis of osteoarthritis and in limiting cartilage repair
    James A Martin
    Department of Orthopaedics, University of Iowa College of Medicine, Iowa City 52242, USA
    J Bone Joint Surg Am 85:106-10. 2003
    ..These observations led us to hypothesize that progressive cell senescence decreases the ability of chondrocytes to maintain and to restore articular cartilage...
  98. ncbi Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
    K F Chilvers
    Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, UK
    J Appl Microbiol 91:1118-30. 2001
  99. ncbi Isolation of the promoter of a cotton beta-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants
    Aimin Wu
    Laboratory of Molecular Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Sci China C Life Sci 49:105-14. 2006
    ..These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development...
  100. ncbi Beta galactosidase complementation: a cell-based luminescent assay platform for drug discovery
    Keith R Olson
    DiscoveRx Corp, Fremont, CA, USA
    Assay Drug Dev Technol 5:137-44. 2007
    ..This review describes the various uses of this flexible technology to cell-based assay development...
  101. ncbi 19F-NMR detection of lacZ gene expression via the enzymic hydrolysis of 2-fluoro-4-nitrophenyl beta-D-galactopyranoside in vivo in PC3 prostate tumor xenografts in the mouse
    Li Liu
    Department of Radiology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390 9058, USA
    FASEB J 21:2014-9. 2007
    ..More importantly, we demonstrate for the first time the ability to differentiate wild-type and lacZ-expressing prostate tumor xenografts in mice using this approach...

Research Grants62

  1. Gene Therapy for Neurodegenerative Lysosomal Storage Diseases
    Douglas R Martin; Fiscal Year: 2013
    ..Validation of this approach to generate AAV vectors with CNS-tropism after iv infusion will pave the way for development of such vectors for human application. ..
  2. Satellite Cell Development and Potential
    David J Goldhamer; Fiscal Year: 2010
    ..abstract_text> ..
  3. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..abstract_text> ..
  4. Regulation of p53 by BRCA1 in Breast Cancer
    JOY CHIEH YU LIN; Fiscal Year: 2013
    ..These novel links between BRCA1 and p53 will help identify those individuals at increased breast cancer risk and to devise more specific therapies. ..
  5. Functional Role of IL-6 in Fetal Brain Development and Abnormal Behavior
    Elaine Hsiao; Fiscal Year: 2012
    ..The long-term goals are to elucidate the molecular mechanisms by which IL-6 causes behavioral abnormalities relevant for autism and schizophrenia. ..
  6. Epithelial Positioning Organization and Ovarian Cancer
    XIANGXI MIKE XU; Fiscal Year: 2010
    ..Such studies will lead to a better understanding of cancer etiology and will contribute to public health by providing cancer preventive stratagy and treatment therapy. ..
  7. Gustatory coding in brainstem nuclei using a novel c-Fos-tau-LacZ mouse model
    JENNIFER STRATFORD; Fiscal Year: 2013
    ..The data obtained from this proposal will provide valuable insight into the spatial and morphological organization of taste representation within the nucleus of the solitary tract. ..
  8. Regulation and function of cdk5 and ezrin in senescence
    Philip W Hinds; Fiscal Year: 2010
    ..3) Ascertain the effects of constitutive cdk5 or ERM "knock-down" or loss on proliferation and tumorigenesis. ..
    Gregory Christman; Fiscal Year: 2001
    ..Together, these studies will provide in vivo efficacy and safety data for the future clinical application of gene therapy in uterine myometrium. ..
    Lee Riley; Fiscal Year: 1999
    ..S. enteritidis infection was selected as the initial mode to evaluate this strategy because of the ready availability of an animal model, knowledge of its genetics, and its importance in human and veterinary public health. ..
    Lee Riley; Fiscal Year: 1999
    ..While this may be a high risk project, the yield, if shown to work, even as a method to enhance mammalian cell delivery of proteins, should have a significant impact. ..
    MARLENE WILSON; Fiscal Year: 2000
    ..It will thus help to elucidate the role(s) of specific, localized neurotransmitter functions in complex behaviors such as anxiety. ..
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
    Kunihiko Suzuki; Fiscal Year: 2001
    ..These point mutations are known in sap B and C in humans causing clinical diseases due to deficiency of the respective activator proteins without affecting processing of other domains. ..
    Thaddeus Golos; Fiscal Year: 2003
    ..abstract_text> ..
    Gerald Pier; Fiscal Year: 1999
    ..epidermidis with either biomaterials or skin affects PS/A production by use of transcriptional fusions of beta galactosidase to PS/A-genes...
    CHARLES SOKOLIK; Fiscal Year: 2001
    ..The utilization of the AFM to probe libraries made by the ink-jet printer technology will provide for a faster, more reliable and reproducible methodology for uncovering the binding characteristics of an antibody. ..
  18. Complementation assay for protein kinase inhibitors
    RICHARD EGLEN; Fiscal Year: 2002
    ..The market for these assays will be in the millions of dollars. ..
    Thaddeus Kurczynski; Fiscal Year: 1980
    ..The effects of psychosine on cellular structure and beta-galactosidase activities will be evaluated in both canine and human fibroblasts...
    KENDRIC SMITH; Fiscal Year: 1990
    ..whether the xmu mutations inhibit induction of the recA gene, using a recA::lacZ fusion and an assay for beta galactosidase activity, and to test whether xmu mutations can be suppressed by mutations in other genes, and to test ..
    Martin Sanda; Fiscal Year: 1999
    ..poxvirus vector vaccination in rat Dunning prostate cancer cells expressing a model target antigen (beta galactosidase); 2) Using a transgenic prostate cancer model (in which SV40 T antigen serves as an androgen-responsive, ..
  22. Gene delivery methods for global therapy in the CNS
    Miguel Esteves; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  23. Estrogen Receptors and Maintenance of Lens Transparency
    Vicki Davis; Fiscal Year: 2005
    ..This study will provide the gateway for future studies to investigate how exposure to various estrogens influence risk of age-related cataracts and the potential of estrogens as a therapy for cataract prevention. ..
    Robert Stroud; Fiscal Year: 1992
    ..Similarities to mammalian rhodopsin and to transmembrane channeling receptors will illuminate processes in vision, neurochemistry and cell-cell regulation...
  25. Development of a High Throughput Genotoxicity Assay
    NEAL HUEBOTTER; Fiscal Year: 2004
    ..abstract_text> ..
    Suzanne Oparil; Fiscal Year: 2003
    ..These fundamental mechanistic studies will establish more rational strategies for therapeutic intervention in vascular diseases, including the basis for future gene therapy. ..
    Kyonggeun Yoon; Fiscal Year: 2001
    ..Gene conversion will be measured at the biochemical, genetic, and molecular level utilizing the shuttle vector system already developed to understand the mechanisms involved in RDO-mediated correction and to optimize RDO design. ..
  28. Engineering Insulin Production by K-Cells to Treat T1DM
    Burton Wice; Fiscal Year: 2004
    ..Results from these pilot studies should provide a sound basis for devising a strategy to isolate, genetically modify and then transplant adult human intestinal epithelial stem cells as a potential treatment for T1DM. ..
  29. Remote Viral Gene Delivery in a Mouse Model of ALS
    Bret Mobley; Fiscal Year: 2003
    ..Data from this study will aid in elucidating the rational choice of viral vector(s) and mode(s) of delivery for optimal expression of therapeutic proteins in the central nervous system of the ALS mouse model. ..
    STEVEN POPOFF; Fiscal Year: 1999
    ..Future studies aimed at developing methods to "block" its osteolytic effect could be used therapeutically to inhibit inflammation- mediated bone loss. ..
  31. Development and use of systems to study estrogen action
    SUSAN NAGEL; Fiscal Year: 2005
  32. A Model System for HIV Multi-Drug Therapy Design
    Chen Chen Kan; Fiscal Year: 2003
    ..This exploratory project marks the first step in establishing a general design strategy for multidrug therapy. ..
    David Pascual; Fiscal Year: 2000
    MALCOLM CASADABAN; Fiscal Year: 1993
    ..Marker enzymes will be selected for their ease of use and ability to function under a wide range of conditions...
  35. Human CD4+ T Cell Based HighThroughput Assay-Drug-R(RMI)
    Chen Chen Kan; Fiscal Year: 2005
    Michael Freeman; Fiscal Year: 2005
    ..5) Determine if Nrf2 must be phosphorylated in order to induce ARE4. This will be approached using biochemical, genetic, and pharmacological techniques. ..
  37. Regulation of Vascular Function by Progesterone Receptor
    Luisa Iruela Arispe; Fiscal Year: 2007
    ..abstract_text> ..
    Gary Smith; Fiscal Year: 1999
    ..The final goal is to characterize, using the technique of differential display, changes in gene expression patterns that accompany the transplantation of tumor cells into different tissue microenvironments. ..
  39. Hepatic engraftment of marrow cells in mice and humans
    Neil Theise; Fiscal Year: 2004
    BRIAN HERRING; Fiscal Year: 2005
    ..The function of SRFs' interaction with these proteins will be elucidated. ..
  41. Non-viral Cutaneous Gene Delivery
    Mohammed Kashani Sabet; Fiscal Year: 2005
    ..We will determine whether efficient expression of the XPC gene in mice will alter the response to UV-mediated DNA damage. ..
    Peter Stambrook; Fiscal Year: 1993
    ..Lastly, a relationship, or absence thereof, can be established between organs and tissues that incur mutations and those that later produce tumors...
    Hung Fan; Fiscal Year: 1999
    ..replication-defective helper-free SIV vectors expressing a readily detectable reporter gene, bacterial beta galactosidase. The vectors will be important for precise elucidation of the pattern of initial infection after vaginal ..
    PYARE KHANNA; Fiscal Year: 2001
    ..This would be a market worth hundreds of millions of dollars. Furthermore, the nuclease assays would be the first such assays for HTS and could open up a new field of drug discovery, garnering even more funds. ..
  45. Nuclear Transcription Factor Translocation Assay
    PYARE KHANNA; Fiscal Year: 2006
    ..In the future, we will adapt this assay as a general technology for drug discovery against any TF to open up an entirely new field of transcription factor Pharmacology and therapeutics. ..
  46. Nuclear Transcription Factor Translocation Assay
    PYARE KHANNA; Fiscal Year: 2003
    ..In the future, we will adapt this assay as a general technology for drug discovery against any TF to open up an entirely new field of transcription factor Dharmacoloav and therapeutics. ..
    JIM KARAM; Fiscal Year: 1992
    ..Antibodies will be generated to different protein segments of the T4 DNA polymerase and used for purification of and analysis of activities intrinsic to mutant peptides. ..
    JAMES DICE; Fiscal Year: 1991
    ..The amount of free ubiquitin and ubiquitin-protein conjugates will be quantified in young and old cells in order to identify the portions of the ubiquitin- mediated degradation pathway that are altered with aging...
    David Borsook; Fiscal Year: 1999
  50. Bone Marrow Cells as Precursors of Alveolar Epithelium
    Alan Fine; Fiscal Year: 2005
    ..These studies, we believe, will help provide a foundation for the application of cell-based therapies for conditions associated with extensive damage to the epithelial surfaces of the lung. ..
  51. Transgenic/SCID-hu Mouse Model to Study HIV Therapeutics
    Harris Goldstein; Fiscal Year: 2005
    ..We will also examine the efficacy of Env-directed toxin-treatment to eliminate reservoirs of HIV-1-infected cells using our well-established thy/liv- SCID-hu mouse system. ..
  52. Cell Based EFC Assay for Protease Drug Discovery
    PYARE KHANNA; Fiscal Year: 2003
    ..When further developed in future phase II SBIR studies, our technology can be used as a general screening assay to discover drugs against most medically important proteases. ..
    Don Wolf; Fiscal Year: 2001
    ..abstract_text> ..
    JAMES DICE; Fiscal Year: 1999
    ..These cell-free systems will permit the detailed biochemical characterization of the age-related defects in the serum-regulated pathway of proteolysis. ..
    ALISON VIGERS; Fiscal Year: 1999
  56. Growth Factor Signaling in Prostate Development & Cancer
    Michael Shen; Fiscal Year: 2004
    ..Our studies should identify critical growth factor pathways for prostate induction and differentiation, and should help elucidate the role of TGF-b signaling in normal prostate development as well as carcinogenesis. ..
    Amy Lee; Fiscal Year: 2006
    KARL WEBER; Fiscal Year: 2002
    ..Insights into these fundamental questions of tissue repair will contribute to prospects for protective interventions that will enable effective management of heart failure of ischemic origin. ..
  59. Estrogen receptor activation in the CNS
    JEFFREY BLAUSTEIN; Fiscal Year: 2005
    ..This initial research is essential for future work on the molecular consequences of ligand independent activation of ERs in the brain of males and females, which can then be studied in an unprecedented way. ..
  60. Cell type gene delivery and alcoholic liver disease
    Michael Wheeler; Fiscal Year: 2006
    ..In addition, through didactic training, and interactions with his mentor and key faculty, the applicant will acquire new skills that will allow him to become a successful member of the alcohol research community. ..
    DALE SANNES GREGERSON; Fiscal Year: 2010
    ..Aim II examines the possibility that local antigen presenting cells are limiting, in number and/or function, thus contributing to retinal immune ignorance and privilege. ..
  62. Local Retinal Antigen Presentation
    DALE SANNES GREGERSON; Fiscal Year: 2010
    ..dendritic cells;or 2. MHC class II on subsets of immune cells in the retina. The ability of circulating antigen presenting cells, or their precursors, to replace these deficiencies in antigen presentation will be tested. ..