sulfatases

Summary

Top Publications

  1. ncbi Catalytic proficiency: the extreme case of S-O cleaving sulfatases
    David R Edwards
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 134:525-31. 2012
  2. ncbi Riding the sulfur cycle--metabolism of sulfonates and sulfate esters in gram-negative bacteria
    M A Kertesz
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092, Zurich, Switzerland
    FEMS Microbiol Rev 24:135-75. 2000
  3. ncbi Multiple sulfatase deficiency is caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme
    Thomas Dierks
    Biochemie II, Universitat Gottingen, Germany
    Cell 113:435-44. 2003
  4. pmc WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier
    Valérie A Schumacher
    Department of Medicine, Children s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Soc Nephrol 22:1286-96. 2011
  5. pmc Sulfatases and a radical S-adenosyl-L-methionine (AdoMet) enzyme are key for mucosal foraging and fitness of the prominent human gut symbiont, Bacteroides thetaiotaomicron
    Alhosna Benjdia
    INRA, UMR1319 MICALIS, F 78352 Jouy en Josas, France
    J Biol Chem 286:25973-82. 2011
  6. pmc The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases
    Gregor Hagelueken
    Division of Structural Biology, German Research Centre for Biotechnology, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 103:7631-6. 2006
  7. ncbi Introducing genetically encoded aldehydes into proteins
    Isaac S Carrico
    Department of Chemistry, Howard Hughes Medical Institute, B84 Hildebrand Hall, University of California, Berkeley, California 94720, USA
    Nat Chem Biol 3:321-2. 2007
  8. ncbi Neural crest migration requires the activity of the extracellular sulphatases XtSulf1 and XtSulf2
    Emily C Guiral
    Department of Biology, University of York, York YO10 5YW, UK
    Dev Biol 341:375-88. 2010
  9. ncbi A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency
    B Schmidt
    Universitat Gottingen, Federal Republic of Germany
    Cell 82:271-8. 1995
  10. ncbi The heparanome--the enigma of encoding and decoding heparan sulfate sulfation
    William C Lamanna
    Department of Chemistry, Biochemistry I, Bielefeld University, Universitatsstr 25, 33615 Bielefeld, Germany
    J Biotechnol 129:290-307. 2007

Detail Information

Publications193 found, 100 shown here

  1. ncbi Catalytic proficiency: the extreme case of S-O cleaving sulfatases
    David R Edwards
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 134:525-31. 2012
    ..Comparison of that value with established k(cat) values of bacterial sulfatases indicates that these enzymes produce rate enhancements (k(cat)/k(uncat)) of up to 2 × 10(26)-fold for the ..
  2. ncbi Riding the sulfur cycle--metabolism of sulfonates and sulfate esters in gram-negative bacteria
    M A Kertesz
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092, Zurich, Switzerland
    FEMS Microbiol Rev 24:135-75. 2000
    ..In these organisms, expression of sulfatases and sulfonatases is repressed in the presence of sulfate, in a process mediated by the LysR-type regulator ..
  3. ncbi Multiple sulfatase deficiency is caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme
    Thomas Dierks
    Biochemie II, Universitat Gottingen, Germany
    Cell 113:435-44. 2003
    C(alpha)-formylglycine (FGly) is the catalytic residue in the active site of eukaryotic sulfatases. It is posttranslationally generated from a cysteine in the endoplasmic reticulum...
  4. pmc WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier
    Valérie A Schumacher
    Department of Medicine, Children s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Soc Nephrol 22:1286-96. 2011
    ....
  5. pmc Sulfatases and a radical S-adenosyl-L-methionine (AdoMet) enzyme are key for mucosal foraging and fitness of the prominent human gut symbiont, Bacteroides thetaiotaomicron
    Alhosna Benjdia
    INRA, UMR1319 MICALIS, F 78352 Jouy en Josas, France
    J Biol Chem 286:25973-82. 2011
    ..To be active, sulfatases must undergo a critical post-translational modification catalyzed in anaerobic bacteria by the radical AdoMet ..
  6. pmc The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases
    Gregor Hagelueken
    Division of Structural Biology, German Research Centre for Biotechnology, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 103:7631-6. 2006
    ..The enzyme SdsA1 thus represents a previously undescribed class of sulfatases that allows P. aeruginosa to survive and thrive under otherwise bacteriocidal conditions.
  7. ncbi Introducing genetically encoded aldehydes into proteins
    Isaac S Carrico
    Department of Chemistry, Howard Hughes Medical Institute, B84 Hildebrand Hall, University of California, Berkeley, California 94720, USA
    Nat Chem Biol 3:321-2. 2007
    ..This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications...
  8. ncbi Neural crest migration requires the activity of the extracellular sulphatases XtSulf1 and XtSulf2
    Emily C Guiral
    Department of Biology, University of York, York YO10 5YW, UK
    Dev Biol 341:375-88. 2010
    ....
  9. ncbi A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency
    B Schmidt
    Universitat Gottingen, Federal Republic of Germany
    Cell 82:271-8. 1995
    ..sulfatase deficiency (MSD) is a lysosomal storage disorder characterized by a decreased activity of all known sulfatases. The deficiency of sulfatases was proposed to result from the lack of a co- or posttranslational modification ..
  10. ncbi The heparanome--the enigma of encoding and decoding heparan sulfate sulfation
    William C Lamanna
    Department of Chemistry, Biochemistry I, Bielefeld University, Universitatsstr 25, 33615 Bielefeld, Germany
    J Biotechnol 129:290-307. 2007
    ..The recent discovery of two sulfatases, Sulf1 and Sulf2, with the unique ability to edit sulfation patterns at the cell surface, has opened up a new ..
  11. ncbi Regulation of Wnt signaling and embryo patterning by an extracellular sulfatase
    G K Dhoot
    Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
    Science 293:1663-6. 2001
    ..avian ortholog of an evolutionarily conserved protein family related to heparan-specific N-acetyl glucosamine sulfatases. QSulf1 expression is induced by Sonic hedgehog in myogenic somite progenitors in quail embryos and is required ..
  12. ncbi Molecular basis for multiple sulfatase deficiency and mechanism for formylglycine generation of the human formylglycine-generating enzyme
    Thomas Dierks
    Department of Biochemistry II, University of Gottingen, D 37073 Gottingen, Germany
    Cell 121:541-52. 2005
    b>Sulfatases are enzymes essential for degradation and remodeling of sulfate esters. Formylglycine (FGly), the key catalytic residue in the active site, is unique to sulfatases...
  13. ncbi SULF1 inhibits tumor growth and potentiates the effects of histone deacetylase inhibitors in hepatocellular carcinoma
    Jin Ping Lai
    Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Gastroenterology 130:2130-44. 2006
    ..Histone deacetylase (HDAC) inhibitors reprogram cellular gene expression through the acetylation of nucleosomal histones and promote cell growth arrest and apoptosis. Hence, they are a promising modality for cancer treatment...
  14. ncbi Mechanistic investigations of anaerobic sulfatase-maturating enzyme: direct Cbeta H-atom abstraction catalyzed by a radical AdoMet enzyme
    Alhosna Benjdia
    INRA, UPR 910, UEPSD, F 78352 Jouy en Josas, France
    J Am Chem Soc 131:8348-9. 2009
    b>Sulfatases are unique in requiring an essential post-translational modification of a critical active-site cysteinyl or seryl residue to 3-oxoalanine usually called C alpha-formylglycine (FGly)...
  15. ncbi Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313
    A Kahnert
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092 Zurich, Switzerland
    J Biol Chem 275:31661-7. 2000
    ..The properties and amino acid sequence of the AtsK enzyme thus define it as an unusual oxygenolytic alkylsulfatase and a novel member of the alpha-ketoglutarate-dependent dioxygenase family...
  16. ncbi New enzymes for biotransformations: microbial alkyl sulfatases displaying stereo- and enantioselectivity
    Petra Gadler
    Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A 8010 Graz, Austria
    Trends Biotechnol 25:83-8. 2007
    ..The latter can be achieved by (alkyl) sulfatases, which can be employed for the enantio-convergent transformation of racemic sulfate esters into a single ..
  17. pmc Type 2 diabetes in mice induces hepatic overexpression of sulfatase 2, a novel factor that suppresses uptake of remnant lipoproteins
    Keyang Chen
    Section of Endocrinology, Diabetes and Metabolism, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Hepatology 52:1957-67. 2010
    ..Adiponectin and insulin each suppressed SULF2 protein in cultured liver cells and in murine livers in vivo, consistent with a role in energy flux. Likewise, both hormones enhanced remnant lipoprotein catabolism in vitro...
  18. pmc Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer
    Junsheng Li
    Department of General Surgery, University of Heidelberg, Heidelberg, Germany
    Mol Cancer 4:14. 2005
    ..Since pancreatic cancers over-express a number of heparin-binding growth factors and their receptors, the expression and function of this enzyme in pancreatic cancer was analyzed...
  19. ncbi Crystal structure of the alkylsulfatase AtsK: insights into the catalytic mechanism of the Fe(II) alpha-ketoglutarate-dependent dioxygenase superfamily
    Ilka Müller
    Lehrstuhl für Strukturchemie, Universitat Gottingen, Tammannstrasse 4, D 37077 Gottingen, Germany
    Biochemistry 43:3075-88. 2004
    ..From comparison of these structures with the crystal structure of AtsK and its complexes, we propose a general mechanism for the catalytic cycle of the alpha-ketoglutarate-dependent dioxygenase superfamily...
  20. ncbi Human sulfatases: a structural perspective to catalysis
    D Ghosh
    Hauptman Woodward Medical Research Institute and Roswell Park Cancer Institute, 700 Ellicott Street, Buffalo, NY 14203, USA
    Cell Mol Life Sci 64:2013-22. 2007
    ..Seventeen genes have been identified in this class of sulfatases, many of which are associated with genetic disorders leading to reduction or loss of function of the ..
  21. ncbi Differential involvement of the extracellular 6-O-endosulfatases Sulf1 and Sulf2 in brain development and neuronal and behavioural plasticity
    Ina Kalus
    Department of Chemistry, Biochemistry I, Bielefeld University, Bielefeld, Germany
    J Cell Mol Med 13:4505-21. 2009
    The extracellular sulfatases Sulf1 and Sulf2 remove specific 6-O-sulfate groups from heparan sulfate, thereby modulating numerous signalling pathways underlying development and homeostasis...
  22. ncbi Sulfs are regulators of growth factor signaling for satellite cell differentiation and muscle regeneration
    Aliete Langsdorf
    Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA
    Dev Biol 311:464-77. 2007
    ..Our results establish Sulfs as essential regulators of HS-dependent growth factor signaling in the adult muscle stem cell niche...
  23. pmc Proteoglycan desulfation determines the efficiency of chondrocyte autophagy and the extent of FGF signaling during endochondral ossification
    Carmine Settembre
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Genes Dev 22:2645-50. 2008
    ..GAGs desulfation, a necessary step for their degradation, is exerted by sulfatases that are activated by another enzyme, Sulfatase-Modifying Factor 1 (SUMF1), whose inactivation in humans leads ..
  24. ncbi Tinkering with heparan sulfate sulfation to steer development
    Bushra Gorsi
    Division of Cardiac and Endocrine Sciences, University of Manchester, UK, M13 9NT
    Trends Cell Biol 17:173-7. 2007
    ..In addition, the latest discovery of HS acting enzymes, the Sulfs, responsible for extracellular tweaking of HS sulfation levels subsequent to biosynthesis will be considered...
  25. pmc Function and structure of a prokaryotic formylglycine-generating enzyme
    Brian L Carlson
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720, USA
    J Biol Chem 283:20117-25. 2008
    Type I sulfatases require an unusual co- or post-translational modification for their activity in hydrolyzing sulfate esters...
  26. pmc Differential sulfation remodelling of heparan sulfate by extracellular 6-O-sulfatases regulates fibroblast growth factor-induced boundary formation by glial cells: implications for glial cell transplantation
    Jennifer R Higginson
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, Glasgow G12 8TA, United Kingdom
    J Neurosci 32:15902-12. 2012
    ..Moreover, these data suggest manipulation of HS sulfation after CNS injury as a potential novel approach for therapeutic intervention in CNS repair...
  27. ncbi Biosynthetic oligosaccharide libraries for identification of protein-binding heparan sulfate motifs. Exploring the structural diversity by screening for fibroblast growth factor (FGF)1 and FGF2 binding
    Per Jemth
    Department of Medical Biochemistry and Microbiology, Uppsala University, Biomedical Center, Box 582, SE 751 23 Uppsala, Sweden
    J Biol Chem 277:30567-73. 2002
    ..FGF1 binding was also augmented by 2-O-sulfation, preferentially in combination with an adjacent upstream 6-O-sulfate group. These results demonstrate the potential of the enzymatically generated oligosaccharide libraries...
  28. ncbi A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes
    Olivier Berteau
    Unité d Ecologie et Physiologie du Système Digestif, Jonas, France
    J Biol Chem 281:22464-70. 2006
    b>Sulfatases are a highly conserved family of enzymes found in all three domains of life...
  29. ncbi New developments in intracrinology of human breast cancer: estrogen sulfatase and sulfotransferase
    Hironobu Sasano
    Department of Pathology Tohoku University School of Medicine, Sendai, Japan
    Ann N Y Acad Sci 1155:76-9. 2009
    ....
  30. pmc Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2
    David H Lum
    Department of Anatomy, HSW 1323, University of California San Francisco, CA 94143 0452, USA
    Mol Cell Biol 27:678-88. 2007
    ..These data suggest that Sulf2 may have roles in several tissues but that there is compensation by and/or redundancy with Sulf1...
  31. ncbi Identification of a novel nonlysosomal sulphatase expressed in the floor plate, choroid plexus and cartilage
    Tatsuyuki Ohto
    Department of Molecular Neurobiology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Genes Cells 7:173-85. 2002
    ..However, the mechanism by which such diversity is generated remains unclear...
  32. pmc The SULFs, extracellular sulfatases for heparan sulfate, promote the migration of corneal epithelial cells during wound repair
    Inna Maltseva
    Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 8:e69642. 2013
    ..Further understanding of the mechanistic action of the SULFs in promoting corneal repair may lead to new therapeutic approaches for the treatment of corneal injuries...
  33. pmc Expression regulation and function of heparan sulfate 6-O-endosulfatases in the spermatogonial stem cell niche
    Aliete Langsdorf
    The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Glycobiology 21:152-61. 2011
    ..Together, our studies not only identify Sulfs as essential regulators of GDNF signaling in the SSC niche, but also as direct downstream targets of WT1, thus establishing a physiological role of WT1 in Sertoli cells...
  34. ncbi Posttranslational modification of serine to formylglycine in bacterial sulfatases. Recognition of the modification motif by the iron-sulfur protein AtsB
    Claudia Marquordt
    Institut fur Biochemie und Molekulare Zellbiologie, Abt Biochemie II, Universitat Gottingen, Heinrich Düker Weg 12, Germany
    J Biol Chem 278:2212-8. 2003
    Calpha-formylglycine is the catalytic residue of sulfatases. Formylglycine is generated by posttranslational modification of a cysteine (pro- and eukaryotes) or serine (prokaryotes) located in a conserved (C/S)XPXR motif...
  35. pmc Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation
    Adam Kleinschmit
    Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, MN 55455, USA
    Dev Biol 345:204-14. 2010
    ..Thus, Sulf1 participates in a feedback loop, potentially stabilizing the shape of the Wg gradient. Our study shows that the modification of HS fine structure provides a novel mechanism for the regulation of morphogen gradients...
  36. ncbi A sulfatase regulating the migratory potency of oligodendrocyte progenitor cells through tyrosine phosphorylation of beta-catenin
    Yoshihiko Kakinuma
    Laboratory for Cell Recovery Mechanisms, Brain Science Institute, RIKEN, Saitama, Japan
    J Neurosci Res 77:653-61. 2004
    ..Thus, the present study revealed that a sulfatase is responsible for the migration of OPCs and activates intracellular mechanisms that regulate migration...
  37. pmc Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways
    Shuhei Otsuki
    Department of Molecular and Experimental Medicine and Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:10202-7. 2010
    ..Approaches to correct abnormal Sulf expression have the potential to protect against cartilage degradation and promote cartilage repair in OA...
  38. ncbi The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases
    Maria Pia Cosma
    Telethon Institute of Genetics and Medicine TIGEM, Naples, Italy
    Cell 113:445-56. 2003
    In multiple sulfatase deficiency (MSD), a human inherited disorder, the activities of all sulfatases are impaired due to a defect in posttranslational modification...
  39. pmc Cloning and characterization of two extracellular heparin-degrading endosulfatases in mice and humans
    Megumi Morimoto-Tomita
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    J Biol Chem 277:49175-85. 2002
    ..cloning of a full-length cDNA encoding the human ortholog (HSulf-1) of the developmentally regulated putative sulfatases QSulf-1 (Dhoot, G. K., Gustafsson, M. K., Ai, X., Sun, W., Standiford, D. M., and Emerson, C. P., Jr...
  40. pmc Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer
    Megumi Morimoto-Tomita
    Department of Anatomy and the UCSF Comprehensive Cancer Center, University of California, San Francisco, 94143 0452, USA
    Neoplasia 7:1001-10. 2005
    ..In support of the possible mechanistic relevance of Sulf-2 upregulation in tumors, purified recombinant Sulf-2 promoted angiogenesis in the chick chorioallantoic membrane assay...
  41. ncbi Sulfatases and human disease
    Graciana Diez-Roux
    Telethon Institute of Genetics and Medicine TIGEM, Department of Pediatrics, Federico II University, Naples 80131, Italy
    Annu Rev Genomics Hum Genet 6:355-79. 2005
    b>Sulfatases are a highly conserved family of proteins that cleave sulfate esters from a wide range of substrates...
  42. ncbi Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
    Sarah R Hanson
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC 357, La Jolla, California 92037, USA
    Angew Chem Int Ed Engl 43:5736-63. 2004
    b>Sulfatases, which cleave sulfate esters in biological systems, play a key role in regulating the sulfation states that determine the function of many physiological molecules...
  43. ncbi Biology and function of the reversible sulfation pathway catalysed by human sulfotransferases and sulfatases
    M W Coughtrie
    Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, UK
    Chem Biol Interact 109:3-27. 1998
    ..system and the enzymes involved, and illustrates how heterologous expression of sulfotransferases (SULTs) and sulfatases is aiding our appreciation of the properties of these important proteins...
  44. pmc A superfamily of metalloenzymes unifies phosphopentomutase and cofactor-independent phosphoglycerate mutase with alkaline phosphatases and sulfatases
    M Y Galperin
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
    Protein Sci 7:1829-35. 1998
    ..phosphatase, alkaline phosphodiesterase/nucleotide pyrophosphatase PC-1, and several closely related sulfatases. In addition to the metal-binding motifs, all these enzymes contain a set of conserved amino acid residues that ..
  45. pmc Systemic inflammation and neurodegeneration in a mouse model of multiple sulfatase deficiency
    Carmine Settembre
    Telethon Institute of Genetics and Medicine TIGEM, Via P Castellino 111, 80131 Naples, Italy
    Proc Natl Acad Sci U S A 104:4506-11. 2007
    b>Sulfatases are involved in several biological functions such as degradation of macromolecules in the lysosomes...
  46. ncbi SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation
    Xingbin Ai
    Boston Biomedical Research Institute, Watertown, MA 02472, USA
    Development 134:3327-38. 2007
    ..These findings provide the first in vivo evidence that Sulfs are essential developmental regulators of cellular HS 6-O-sulfation for matrix transmission and reception of GDNF signal from muscle to innervating neurons...
  47. pmc Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells
    Roman Nawroth
    Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e392. 2007
    ..Sulf-1 and Sulf-2 are novel extracellular sulfatases that act on internal glucosamine-6-sulfate (6S) modifications within HSPGs and thereby modulate HSPG ..
  48. pmc Purification and characterization of an inverting stereo- and enantioselective sec-alkylsulfatase from the gram-positive bacterium Rhodococcus ruber DSM 44541
    Mateja Pogorevc
    Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A 8010 Graz, Austria
    Appl Environ Microbiol 69:2810-5. 2003
    ..Due to the fact that prim-sulfates were not accepted, the enzyme was classified as sec-alkylsulfatase (EC 3.1.6.X)...
  49. ncbi Inhibition of phosphatase and sulfatase by transition-state analogues
    P J Stankiewicz
    Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    Biochemistry 27:206-12. 1988
    ..It is apparent that the effectiveness of metal oxyanions as inhibitors of phosphatases and sulfatases can be selectively enhanced in the presence of other solutes...
  50. ncbi Sulf loss influences N-, 2-O-, and 6-O-sulfation of multiple heparan sulfate proteoglycans and modulates fibroblast growth factor signaling
    William C Lamanna
    Fakultat fur Chemie, Biochemie I, Universitat Bielefeld, 33615 Bielefeld, Germany
    J Biol Chem 283:27724-35. 2008
    ....
  51. ncbi Sulfotransferases, sulfatases and formylglycine-generating enzymes: a sulfation fascination
    Pavla Bojarová
    School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melboume, Parkville, Victoria 3010, Australia
    Curr Opin Chem Biol 12:573-81. 2008
    Sulfotransferases and sulfatases are the major enzymes responsible for sulfate transfer processes...
  52. pmc Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity
    William C Lamanna
    Department of Chemistry, Biochemistry I, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Biochem J 400:63-73. 2006
    ....
  53. ncbi Crystal structure of human pFGE, the paralog of the Calpha-formylglycine-generating enzyme
    Achim Dickmanns
    Abteilung Molekulare Strukturbiologie, Institut fur Mikrobiologie und Genetik, Georg August Universitat, Justus von Liebig Weg 9, D 37077 Gottingen, Germany
    J Biol Chem 280:15180-7. 2005
    In eukaryotes, sulfate esters are degraded by sulfatases, which possess a unique Calpha-formylglycine residue in their active site...
  54. ncbi Domain-specific modification of heparan sulfate by Qsulf1 modulates the binding of the bone morphogenetic protein antagonist Noggin
    Beth L Viviano
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, St Louis, Missouri 63110, USA
    J Biol Chem 279:5604-11. 2004
    ....
  55. ncbi Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development
    Andreas Ratzka
    Center for Medical Biotechnology, University of Duisburg Essen, Essen, Germany
    Dev Dyn 237:339-53. 2008
    ..The secreted sulfatases, Sulf1 and Sulf2, desulfate HS on 6-O-positions extracellularly...
  56. pmc QSulf1 remodels the 6-O sulfation states of cell surface heparan sulfate proteoglycans to promote Wnt signaling
    Xingbin Ai
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Cell Biol 162:341-51. 2003
    ....
  57. pmc QSulf1, a heparan sulfate 6-O-endosulfatase, inhibits fibroblast growth factor signaling in mesoderm induction and angiogenesis
    Shouwen Wang
    Department of Cell and Developmental Biology, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 101:4833-8. 2004
    ....
  58. pmc Secreted sulfatases Sulf1 and Sulf2 have overlapping yet essential roles in mouse neonatal survival
    Charles R Holst
    Department of Molecular Oncology, Genentech, Inc, South San Francisco, California, United States of America
    PLoS ONE 2:e575. 2007
    ..Various sulfotransferases and sulfatases with differing specificities control the pattern of HSPG sulfation, which is functionally critical...
  59. ncbi Molecular cloning and initial characterization of three novel human sulfatases
    Alvaro J Obaya
    Departamento de Biologia Funcional, Area de Fisiologia, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Gene 372:110-7. 2006
    b>Sulfatases constitute a group of enzymes capable of hydrolyzing the sulphate ester bond of a variety of biological compounds. To date, thirteen members of this family have been cloned and characterized as part of the human genome...
  60. ncbi Substrate specificity and domain functions of extracellular heparan sulfate 6-O-endosulfatases, QSulf1 and QSulf2
    Xingbin Ai
    Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA
    J Biol Chem 281:4969-76. 2006
    The extracellular sulfatases (Sulfs) are an evolutionally conserved family of heparan sulfate (HS)-specific 6-O-endosulfatases...
  61. pmc Sulfatase modifying factor 1 trafficking through the cells: from endoplasmic reticulum to the endoplasmic reticulum
    Ester Zito
    Telethon Institute of Genetics and Medicine TIGEM, Naples, Italy
    EMBO J 26:2443-53. 2007
    ..sulfatase deficiency (MSD) that encodes the formylglycine-generating enzyme, an essential activator of all the sulfatases. SUMF1 is a glycosylated enzyme that is resident in the endoplasmic reticulum (ER), although it is also secreted...
  62. ncbi Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship
    M Sardiello
    Telethon Institute of Genetics and Medicine, Naples, Italy
    Hum Mol Genet 14:3203-17. 2005
    b>Sulfatases catalyze the hydrolysis of sulfate ester bonds from a wide variety of substrates...
  63. ncbi Deficiencies of glucosamine-6-sulfate or galactosamine-6-sulfate sulfatases are responsible for different mucopolysaccharidoses
    N Di Ferrante
    Laboratories of Connective Tissue Research, Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Science 199:79-81. 1978
    ..These findings demonstrate the existence of two different hexosamine-6-sulfate sulfatases, specific for the glucose or galactose configuration of their substrates...
  64. pmc A general binding mechanism for all human sulfatases by the formylglycine-generating enzyme
    Dirk Roeser
    Department of Molecular Structural Biology, University of Gottingen, Justus von Liebig Weg 11, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:81-6. 2006
    ..FGly)-generating enzyme (FGE) uses molecular oxygen to oxidize a conserved cysteine residue in all eukaryotic sulfatases to the catalytically active FGly...
  65. ncbi Synthetic potential of molluscan sulfatases for the library synthesis of regioselectively O-sulfonated D-galacto-sugars
    Hirotaka Uzawa
    Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology AIST, Central 5, 11 1 Higashi, Tsukuba 305 8565, Japan
    Chembiochem 4:640-7. 2003
    The substrate specificities of three molluscan sulfatases (E.C. 3.1.6...
  66. ncbi Molecular and functional analysis of SUMF1 mutations in multiple sulfatase deficiency
    Maria Pia Cosma
    Telethon Institute of Genetics and Medicine, Naples, Italy
    Hum Mutat 23:576-81. 2004
    Multiple sulfatase deficiency (MSD) is a rare disorder characterized by impaired activity of all known sulfatases. The gene mutated in this disease is SUMF1, which encodes a protein involved in a post-translational modification at the ..
  67. ncbi Sulfotransferases and sulfatases in mycobacteria
    Joseph D Mougous
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Chem Biol 9:767-76. 2002
    ..The sulfotransferases join an equally complex family of mycobacterial sulfatases: a large family of sulfatase genes has been found in all of the mycobacterial genomes examined...
  68. ncbi The sulfatase gene family
    G Parenti
    Department of Pediatrics, Federico II University, Naples, Italy
    Curr Opin Genet Dev 7:386-91. 1997
    ..These discoveries, together with the resolution of the crystallographic structure of sulfatases, have improved our understanding of the function and evolution of this fascinating family of enzymes.
  69. ncbi Anaerobic sulfatase-maturating enzymes: radical SAM enzymes able to catalyze in vitro sulfatase post-translational modification
    Alhosna Benjdia
    INRA, Unité d Ecologie et Physiologie du Système Digestif, 78352 Jouy en Josas, France
    J Am Chem Soc 129:3462-3. 2007
  70. ncbi The heparin/heparan sulfate 2-O-sulfatase from Flavobacterium heparinum. A structural and biochemical study of the enzyme active site and saccharide substrate specificity
    Rahul Raman
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 278:12167-74. 2003
    ..This combinatorial approach of structure modeling and biochemical studies provides insight into the molecular basis of enzyme function...
  71. ncbi Measuring the activities of the Sulfs: two novel heparin/heparan sulfate endosulfatases
    Kenji Uchimura
    Department of Anatomy, Program in Immunology, UCSF, USA
    Methods Enzymol 416:243-53. 2006
    b>Sulfatases hydrolyze sulfate esters on a variety of molecules including glycosaminoglycans, sulfoglycolipids, and cytosolic steroids...
  72. ncbi Phytoestrogens are potent inhibitors of estrogen sulfation: implications for breast cancer risk and treatment
    R M Harris
    School of Biosciences and Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Clin Endocrinol Metab 89:1779-87. 2004
    ..Hence, dietary flavonoids may be able to influence the bioavailability of endogenous estrogens, and disrupt endocrine balance, by increasing the ratio of active estrogens to inactive estrogen sulfates in human tissues...
  73. ncbi Dydrogesterone (Duphaston) and its 20-dihydro-derivative as selective estrogen enzyme modulators in human breast cancer cell lines. Effect on sulfatase and on 17beta-hydroxysteroid dehydrogenase (17beta-HSD) activity
    Gerard Samuel Chetrite
    Hormones and Cancer Research Unit, Institut de Puericulture, 26 Boulevard Brune, 75014 Paris, France
    Anticancer Res 24:1433-8. 2004
    ..Duphaston is a progestogen with properties similar to the endogenous progesterone. The data open interesting perspectives to study the biological responses of these progestogens in clinical trials of patients with breast cancer...
  74. ncbi Effect of nomegestrol acetate on estrogen biosynthesis and transformation in MCF-7 and T47-D breast cancer cells
    J Shields-Botella
    Non Clinical Research and Development Department, Théramex, 6 Avenue Prince Hereditaire Albert, 98000, Monaco
    J Steroid Biochem Mol Biol 93:1-13. 2005
    ....
  75. ncbi The origin of oestrone sulphate in normal and malignant breast tissues in postmenopausal women
    A Purohit
    Unit of Metabolic Medicine, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Horm Metab Res 24:532-6. 1992
    ..It is concluded that oestrone sulphate, as such, is not taken up by breast tissues and that any contribution that oestrone sulphate makes to the oestrogen content of breast tissues will depend upon prior hydrolysis...
  76. ncbi Structural characterization of heparan sulfate and chondroitin sulfate of syndecan-1 purified from normal murine mammary gland epithelial cells. Common phosphorylation of xylose and differential sulfation of galactose in the protein linkage region tetrasa
    M Ueno
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan
    J Biol Chem 276:29134-40. 2001
    ....
  77. ncbi Some new aspects of 17alpha-estradiol metabolism in man
    Gerhard Hobe
    Hans Knöll Institute for Natural Products Research, Beutenberg Str 11, 07745 Jena, Germany
    Steroids 67:883-93. 2002
    ..From this, it has to be concluded that the serum conjugates were deconjugated and newly reconjugated before urinary excretion...
  78. ncbi Inhibition of oestrone sulphatase activity by tibolone and its metabolites
    A Purohit
    Dept of Endocrinology and Metabolic Medicine, Imperial College School of Medicine, St Mary s Hospital, London, W2 1NY, UK
    Horm Metab Res 34:1-6. 2002
    ..Results from this study have confirmed that tibolone and its metabolites can inhibit E1-STS activity. This may explain the absence of breast stimulation as observed in clinical studies...
  79. ncbi Estrone 3-sulfate mimics, inhibitors of estrone sulfatase activity: homology model construction and docking studies
    Nicola M Howarth
    Medicinal Chemistry, Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, UK
    Biochemistry 41:14801-14. 2002
    ..Docking studies using the substrate and estrone-3-sulfate mimics that are active inhibitors indicate they are positioned in the area of proposed catalysis, confirming the predictive power of the model...
  80. ncbi Steroidal oxathiazine inhibitors of estrone sulfatase
    Richard H Peters
    SRI International, Life Science Division, 333 Ravenswood Avenue Bldg, 100 2, Menlo Park, CA 94025, USA
    Steroids 68:97-110. 2003
    ..The synthesis and biological activity of a number of these unique steroidal ESIs are described...
  81. ncbi Enzymatic synthesis of chondroitin sulfate E by N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase purified from squid cartilage
    Osami Habuchi
    Department of Life Science, Aichi University of Education, Igaya cho, Kariya, Aichi 448 8542, Japan
    Anal Biochem 310:129-36. 2002
    ..Squid GalNAc4S-6ST thus should provide a useful tool for preparing CS-E and oversulfated DS with a defined proportion of GalNAc(4,6-SO(4)) residues...
  82. ncbi Sulfated steroids as endogenous neuromodulators
    Terrell T Gibbs
    Laboratory of Molecular Neurobiology, Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, United States
    Pharmacol Biochem Behav 84:555-67. 2006
    ..The sites to which neuroactive steroids bind may also serve as targets for the discovery of therapeutic neuromodulators...
  83. pmc Syndecan-1: a dynamic regulator of the myeloma microenvironment
    Ralph D Sanderson
    Department of Pathology, Division of Cellular and Molecular Pathology, University of Alabama at Birmingham, 814 Shelby Building, 1530 Third Avenue South, Birmingham, AL 35294 2182, USA
    Clin Exp Metastasis 25:149-59. 2008
    ..This strategy of targeting heparan sulfate may be particularly effective for attacking cancers like myeloma where extensive genetic chaos renders them unlikely to respond well to agents that target a single signaling pathway...
  84. ncbi Progestins and breast cancer
    Jorge R Pasqualini
    Hormones and Cancer Research Unit, Institut de Puériculture et de Périnatalogie, Paris, France
    Gynecol Endocrinol 23:32-41. 2007
    ....
  85. ncbi Biological effects of progestins in breast cancer
    J R Pasqualini
    Hormones and Cancer Research Unit, , Paris, France
    Gynecol Endocrinol 15:44-52. 2001
    ..Consequently, the action of progestins in blocking estradiol formation via sulfatase, or in stimulating the effect on sulfotransferase activity, can open interesting and new possibilities in clinical applications in breast cancer...
  86. ncbi Differential effects of progestins on breast tissue enzymes
    J R Pasqualini
    Hormones and Cancer Research Unit, Institut de Puericulture, 26 Blvd Brune, Paris 75014, France
    Maturitas 46:S45-54. 2003
    ..Some progestins and tibolone can also stimulate sulfotransferase activity. These various progestins may therefore provide a new option for the treatment of breast cancer...
  87. ncbi Pharmacokinetics of the glucuronide and sulfate conjugates of genistein and daidzein in men and women after consumption of a soy beverage
    Susan R Shelnutt
    Arkansas Children s Nutrition Center and the Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72202, USA
    Am J Clin Nutr 76:588-94. 2002
    ..Isoflavone conjugates may serve as sources of aglycones at specific target tissues and may have bioactivity. Yet, very little is known about the plasma pharmacokinetics of isoflavone conjugates after soy ingestion...
  88. ncbi Fate of 3,3'-diindolylmethane in cultured MCF-7 human breast cancer cells
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Chem Res Toxicol 19:436-42. 2006
    ..These findings show that the uptake of DIM by membranes significantly increases its localized concentration, which may contribute to its biological activities...
  89. ncbi Metabolism of pyrene by aquatic crustacean, Daphnia magna
    Yoshinori Ikenaka
    Division of Science for Inland Water Environment, Institute of Mountain Science, Shinshu University, 5 2 4 Kogandori Suwa, Nagano 392 0027, Japan
    Aquat Toxicol 80:158-65. 2006
    ..These results indicated that CYP and several conjugation enzymes participate in its biotransformation, and sulfation is important in D. magna for metabolism and elimination of xenobiotics...
  90. ncbi Compositional profiling of heparin/heparan sulfate using mass spectrometry: assay for specificity of a novel extracellular human endosulfatase
    Ola M Saad
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Glycobiology 15:818-26. 2005
    ..Proof of principle was performed by using various heparin/HS samples isolated from bovine and porcine tissues...
  91. ncbi Evidence for sulfatase and 17beta-hydroxysteroid dehydrogenase type 1 activities in equine epididymis and uterus
    Emmanuel Lemazurier
    Laboratory of Biochemistry and Molecular Biology, University of Caen, France
    Theriogenology 58:113-21. 2002
    ..Because E2 appears to play a major role in male and female reproduction, we propose that the bioconversion of seminal E1S could affect male and female fertility...
  92. ncbi The heparin/heparan sulfate 2-O-sulfatase from Flavobacterium heparinum. Molecular cloning, recombinant expression, and biochemical characterization
    James R Myette
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 278:12157-66. 2003
    ..Finally, we describe the substrate-product relationship of the 2-O-sulfatase to the Delta4,5-glycuronidase and the analytical value of using both of these enzymes in tandem for elucidating heparin/heparan sulfate composition...
  93. ncbi Caprine mucopolysaccharidosis IIID: fetal and neonatal brain and liver glycosaminoglycan and morphological perturbations
    Margaret Z Jones
    Division of Human Pathology, Colleges of Human and Osteopathic Medicine, Michigan State University, East Lansing, MI 48824, USA
    J Mol Neurosci 24:277-91. 2004
    ..Based on the time frame of development of CNS lesions and biochemical perturbations, prenatal therapeutic intervention in caprine MPS IIID is likely to be necessary to prevent or ameliorate substantive CNS and systemic lesions...
  94. ncbi Synthesis and biochemical evaluation of novel and potent inhibitors of the enzyme oestrone sulphatase (ES)
    Sabbir Ahmed
    School of Chemical and Pharmaceutical Sciences, Kingston University, Penrhyn Road, Kingston upon Thames, Surrey KT1 2EE, UK
    J Steroid Biochem Mol Biol 80:419-27. 2002
    ..5 aids the inhibition through the rapid expulsion of the carbon backbone from the active site. We also propose that the same factor is responsible for the hydrolysis of oestrone sulphate reaction, appearing to be an irreversible process...
  95. ncbi Estradiol as an anti-aromatase agent in human breast cancer cells
    J R Pasqualini
    Hormones and Cancer Research Unit, Institut de Puériculture et de Périnatalogie, 26 Blvd Brune, 75014 Paris, France
    J Steroid Biochem Mol Biol 98:12-7. 2006
    ..The dual inhibition of the aromatase and sulfatase activities, two crucial enzymes for the biosynthesis of E(2) by E(2) itself in breast cancer add interesting and attractive information for the use of estrogen therapeutic treatments...
  96. ncbi Design, synthesis and biochemical evaluation of AC ring mimics as novel inhibitors of the enzyme estrone sulfatase (ES)
    Sabbir Ahmed
    School of Chemical and Pharmaceutical Sciences, Kingston University, Penrhyn Road, Kingston upon Thames, Surrey KT1 2EE, UK
    J Steroid Biochem Mol Biol 82:425-35. 2002
    ..Furthermore, the compounds are observed to be irreversible inhibitors...
  97. ncbi Genomic basis of mucopolysaccharidosis type IIID (MIM 252940) revealed by sequencing of GNS encoding N-acetylglucosamine-6-sulfatase
    Andrea Mok
    Robarts Research Institute, London, Ontario, Canada N6A 5K8
    Genomics 81:1-5. 2003
    ..This first report of a mutation in GNS resulting in MPS IIID indicates the potential utility of molecular diagnosis for this rare condition...
  98. ncbi The design, synthesis, and biochemical evaluation of derivatives of biphenyl sulfamate-based compounds as novel inhibitors of estrone sulfatase
    Sabbir Ahmed
    School of Chemical and Pharmaceutical Sciences, Kingston University, Penrhyn Road, Kingston upon Thames, Surrey KT1 2EE, UK
    Biochem Biophys Res Commun 294:180-3. 2002
    ....
  99. ncbi Purification, characterization and crystallization of human placental estrone/dehydroepiandrosterone sulfatase, a membrane-bound enzyme of the endoplasmic reticulum
    F G Hernandez-Guzman
    Department of Molecular and Cellular Biophysics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    J Steroid Biochem Mol Biol 78:441-50. 2001
    ..This work paves the way for the first structural study of a membrane bound human sulfatase and subsequent rational design of inhibitors for use as anti-tumor agents...
  100. ncbi Ramipril and aminoguanidine restore renal lysosomal processing in streptozotocin diabetic rats
    T M Osicka
    Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia
    Diabetologia 44:230-6. 2001
    ....
  101. pmc Genetic analysis of murine arylsulfatase C and steroid sulfatase
    B M Keinanen
    Genetics 105:191-206. 1983
    ..Positive correlation between hepatic and renal 4MUS-sulfatase activities indicates that both activities are most likely influenced by the same gene...

Research Grants32

  1. Role of Heparan Sulfate-Degrading Sulfatases in Pancreatic Adenocarcinomas
    Steven Rosen; Fiscal Year: 2007
    ..Our exploratory R21 project proposes to investigate the role of two novel sulfatases, called HSulf-1 and HSulf-2, in Wnt signaling within pancreatic adenocarcinomas and their contributions to the ..
  2. Extracellular Sulfatases As Novel Biomarkers For Early Detection of Cancer
    Joanna Phillips; Fiscal Year: 2013
    ....
  3. Disposition of Flavonoids via Metabolic Interplay
    Ming Hu; Fiscal Year: 2012
    ..be bioavailable since local and systemic hydrolysis of conjugated flavonoids to aglycones by glucuronidases and sulfatases is a viable means of providing aglycone to target organs...
  4. CONTROL OF MUSCLE PROTEIN SYNTHESIS DURING MYOGENESIS
    Charles P Emerson; Fiscal Year: 2010
    ..These studies will focus on a family of two novel Sulfatases that were identified in our screen for somite-activated genes and are required for activation of MyoD in somites ..
  5. Expression of Heparan Sulfate 6-O-Endosulfatases in Idiopathic Pulmonary Fibrosis
    Xinping Yue; Fiscal Year: 2010
    ..TGF-21 induces heparan sulfate (HS) 6-O-endosulfatase 1 and 2 (Sulf1 and Sulf2), which are novel cell surface sulfatases that remove the 6-O-sulfates from specific HS intra-chain sites...
  6. Mucosal Immune Barrier in Infection and Immunity
    Keith E Mostov; Fiscal Year: 2012
    ..Projects 1 (Mostov), 2 (Engel) and 3 (Rosen) study mechanisms of epithelial wound healing, the roles of sulfs (sulfatases which modify heparan sulfate proteoglycans) in epithelial response to injury and the interaction of polarized ..
  7. MOLECULAR PATHOGENESIS OF HEPATOCELLULAR CARCINOMA
    Lewis Roberts; Fiscal Year: 2009
    ..These sulfatases modify the binding of heparin-binding growth factors to their receptors on the cell surface or in the ..
  8. Sulfatase Activated Fluorescent Probes for In Vivo Diagnostic Imaging of Cancer
    KIMBERLY ELIZABETH BEATTY; Fiscal Year: 2010
    ..The expression levels of the recently characterized sulfatases HSulf-1 and HSulf-2 are increased in certain cancers (e.g...
  9. Structure-Function Relationship of Glycosaminoglycans
    Ram Sasisekharan; Fiscal Year: 2013
    ..many of these enzymes, and other GAG or glycan binding proteins, have poor sequence identity [this includes key sulfatases, fibroblast growth factors or influenza virus hemagglutinin (HA)], we developed newer tools to study GAG-protein ..
  10. Post-biosynthetic remodeling of heparan sulfate
    Joseph Zaia; Fiscal Year: 2013
    ..Aim 3 is to determine the manner in which extracellular enzymes (mammalian endo- sulfatases and heparanase) remodel HS chains in biological systems...
  11. DEFINING HOST-MICROBIAL INTERACTIONS THAT TRIGGER INFLAMMATORY BOWEL DISEASE
    Thaddeus S Stappenbeck; Fiscal Year: 2013
    ..We have preliminary data to show that deletion of a single gene in B. theta that controls the activity of 28 sulfatases (anSME for anaerobic sulfatase maturating enzyme) completely inhibits colitis in colonized dnKO mice...
  12. BIOCHEMICAL BASIS OF SULFATASE ACTIVITY
    ALLEN HORWITZ; Fiscal Year: 1992
    Mammalian sulfatases are a diverse group of enzymes which hydrolize steroid, mucopolysaccharide and glycolipid sulfates...
  13. MOLECULAR GENETICS OF IDURONATE SULFATASE DEFICIENCIES
    Joseph Muenzer; Fiscal Year: 1991
    ..Iduronate sulfatase is also known to be decreased in multiple sulfatase deficiency along with at least six other sulfatases. This disorder combines features of MPS and metachromatic leukodystrophy...
  14. SULFONYL ANALOGUES OF SULFATES AND PHOSPHATES
    THEODORE WIDLANSKI; Fiscal Year: 1993
    ..Their ability to inhibit sulfatases and processes dependant on sulfatases, such as fertilization will be tested...
  15. Genetic Variation in Estrogen Sulfation Genes and Endometrial Cancer Risk
    VERONICA SETIAWAN; Fiscal Year: 2009
    ..Estrogen sulfatases, on the other hand, hydrolyze biologically inactive estrogen-sulfate to active estrogens...
  16. ISOLATION AND CLONING OF GLYCOSAMINOGLYCAN SULFATASES
    Ganesh Venkataraman; Fiscal Year: 1999
    DESCRIPTION: (adapted from applicant's abstract) The goal of this grant application is to isolate and clone the sulfatases of heparin-like glycosaminoglycans...
  17. Sulfotransferases in the Synthesis of L-Selectin Ligands
    Steven D Rosen; Fiscal Year: 2010
    ..Understanding this mechanism may lead to new pharmacologic approaches for dampening eosinophil responses. ..
  18. MAMMALIAN SPERM SURFACE MANNOSIDASE
    DAULAT TULSIANI; Fiscal Year: 2003
    ....
  19. CHOLESTEROL TRANSPORT IN GALLBLADDER EPITHELIAL CELLS
    Rahul Kuver; Fiscal Year: 2003
    ..3. Compare and contrast the mechanisms of activation of ABCA1 via the cAMP responsive and the LXRalpha/RXR- responsive pathways. ..
  20. STRUCTURE/FUNCTION OF ZONA PELLUCIDA GLYCOPROTEINS
    DAULAT TULSIANI; Fiscal Year: 2001
    ....
  21. PATHOGENESIS OF DISEASE IN MUCOPOLYSACCHARIDOSIS I AND VII
    Katherine P Ponder; Fiscal Year: 2010
    ..These studies may demonstrate that the development of disease in the aorta and the cervical spine in MPS is due to a common mechanism, and may identify a therapy for these difficult-to-treat sites. ..
  22. Structure of Microsomal Enzymes in Estrogen Biosynthesis
    Debashis Ghosh; Fiscal Year: 2005
    ....
  23. GENE THERAPY FOR BLOOD PROTEIN DEFICIENCIES
    KATHERINE PONDER; Fiscal Year: 2005
    ..Success in this project might lead to a safe, effective, and permanent therapy for Hemophilia B. ..
  24. Developmental control of cell polarity in vertebrate embryos.
    John B Wallingford; Fiscal Year: 2010
    ..Together, these experiments will help us to understand how PCP signaling influences such diverse cellular processes as convergent extension and ciliogenesis. ..
  25. NATURAL VITAMIN E FORMS AS ANTI-INFLAMMATORY DRUGS
    Qing Jiang; Fiscal Year: 2006
    ..abstract_text> ..
  26. Biliary oxysterols and cholangiocarcinoma
    RAHUL P KUVER; Fiscal Year: 2010
    ..abstract_text> ..
  27. IMPROVED METHODS FOR IN VIVO HEPATIC GENE THERAPY
    KATHERINE PONDER; Fiscal Year: 2006
    ..Success in this project might lead to a safe, effective, and permanent therapy for genetic deficiencies that involve proteins synthesized by the liver. ..
  28. MUCIN SECRETION IN COLUMNAR EPITHELIAL CELLS
    Rahul Kuver; Fiscal Year: 2003
    ..The sponsor's group's expertise in biliary cell biology will ensure a supportive and stimulating environment for the candidate. ..
  29. Role of heparanase in osteolytic bone metastasis
    Ralph Sanderson; Fiscal Year: 2007
    ..abstract_text> ..
  30. SYNDECAN FUNCTION ON MYELOMA CELLS
    Ralph Sanderson; Fiscal Year: 2004
    ..Insight gained from these studies will be used to design new therapeutic strategies aimed at halting tumor growth and metastasis. ..
  31. SULFOTRANSFERASE EXPRESSION IN EPITHELIA AND CARCINOMAS
    THOMAS DOOLEY; Fiscal Year: 2001
    ..The anticipated results will provide valuable insights into the roles of the human ST gene superfamily in the metabolism and bioavailability of drugs and endogenous compounds in human epithelia and cancers. ..
  32. Ocular Albinism type 1: from molecular bases to gene delivery
    Andrea Ballabio; Fiscal Year: 2007
    ..Finally, the gene delivery approaches developed may represent important tools for future treatment of eye diseases involving RPE. ..