ribonucleases

Summary

Summary: Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.

Top Publications

  1. ncbi GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell 44:120-33. 2011
  2. ncbi Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis
    Marcin Nowotny
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 121:1005-16. 2005
  3. ncbi Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay
    Kazufumi Matsushita
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nature 458:1185-90. 2009
  4. pmc Deadenylation is a widespread effect of miRNA regulation
    Ana Eulalio
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    RNA 15:21-32. 2009
  5. pmc The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release
    Latifa Zekri
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell Biol 29:6220-31. 2009
  6. pmc The unfolded protein response signals through high-order assembly of Ire1
    Alexei V Korennykh
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Nature 457:687-93. 2009
  7. ncbi The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array
    Vickery L Arcus
    Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand
    Protein Eng Des Sel 24:33-40. 2011
  8. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
  9. pmc MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-kappaB signaling
    Jian Liang
    Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA
    J Exp Med 207:2959-73. 2010
  10. ncbi Molecular basis for the recognition and cleavage of RNA by the bifunctional 5'-3' exo/endoribonuclease RNase J
    Audrey Dorleans
    FRC550, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Structure 19:1252-61. 2011

Research Grants

Detail Information

Publications370 found, 100 shown here

  1. ncbi GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell 44:120-33. 2011
    ....
  2. ncbi Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis
    Marcin Nowotny
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 121:1005-16. 2005
    ..In transposases, they are symmetrically coordinated and exchange roles to alternately activate a water and a 3'-OH for successive strand cleavage and transfer by a ping-pong mechanism...
  3. ncbi Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay
    Kazufumi Matsushita
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nature 458:1185-90. 2009
    ..Together, these results indicate that Zc3h12a is an essential RNase that prevents immune disorders by directly controlling the stability of a set of inflammatory genes...
  4. pmc Deadenylation is a widespread effect of miRNA regulation
    Ana Eulalio
    Max Planck Institute for Developmental Biology, D 72076 Tubingen, Germany
    RNA 15:21-32. 2009
    ..These results indicate that miRNAs promote mRNA decay by altering mRNP composition and/or conformation, rather than by directly interfering with the binding and function of ribosomal subunits...
  5. pmc The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release
    Latifa Zekri
    Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell Biol 29:6220-31. 2009
    ..We propose a model in which the GW182 silencing domain promotes translational repression, at least in part, by interfering with mRNA circularization and also recruits the deadenylase complex through the interaction with PABPC1...
  6. pmc The unfolded protein response signals through high-order assembly of Ire1
    Alexei V Korennykh
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Nature 457:687-93. 2009
    ..Activation of Ire1 through oligomerization expands the mechanistic repertoire of kinase-based signalling receptors...
  7. ncbi The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array
    Vickery L Arcus
    Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand
    Protein Eng Des Sel 24:33-40. 2011
    ..PIN-domains cleave single-stranded RNA in a sequence-specific, Mg²+- or Mn²+-dependent manner. These ribonucleases are toxic to the cells which express them and to offset this toxicity, they are co-expressed with tight ..
  8. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
    ..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
  9. pmc MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-kappaB signaling
    Jian Liang
    Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA
    J Exp Med 207:2959-73. 2010
    ..Sequence analysis together with serial mutagenesis defined a deubiquitinating enzyme domain and a ubiquitin association domain in MCPIP1. Our results indicate that MCPIP1 is a critical modulator of inflammatory signaling...
  10. ncbi Molecular basis for the recognition and cleavage of RNA by the bifunctional 5'-3' exo/endoribonuclease RNase J
    Audrey Dorleans
    FRC550, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Structure 19:1252-61. 2011
    ..We propose a model involving the binding of the RNA to the surface of the β-CASP domain to explain the enzyme's processive action...
  11. pmc An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis
    Jamie Richards
    Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Microbiology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Mol Cell 43:940-9. 2011
    ..This pathway, which closely resembles the mechanism by which deadenylated mRNA is degraded in eukaryotic cells, explains the stabilizing influence of 5'-terminal stem-loops in such bacteria...
  12. ncbi The tandem affinity purification (TAP) method: a general procedure of protein complex purification
    O Puig
    European Molecular Biology Laboratory Meyerhofstrasse 1, Heidelberg, D-69117, Germany
    Methods 24:218-29. 2001
    ..Its simplicity, high yield, and wide applicability make the TAP method a very useful procedure for protein purification and proteome exploration...
  13. ncbi Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction
    Jerome Basquin
    Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 48:207-18. 2012
    ..Thus, the N-terminal arm of Not1 forms an extended platform reminiscent of scaffolding proteins like eIF4G and CBP80, and places the two nucleases in a pivotal position within the Ccr4-Not complex...
  14. ncbi Structural insights into the dual activity of RNase J
    Ines Li de La Sierra-Gallay
    FRC550, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Nat Struct Mol Biol 15:206-12. 2008
    ....
  15. ncbi Unusual, dual endo- and exonuclease activity in the degradosome explained by crystal structure analysis of RNase J1
    Joseph A Newman
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Structure 19:1241-51. 2011
    ..Comparisons of the B. subtilis RNase J structure with related enzymes reveal key differences that provide insights into conformational changes during catalysis and the role of the C-terminal domain...
  16. pmc Depletion of mammalian CCR4b deadenylase triggers elevation of the p27Kip1 mRNA level and impairs cell growth
    Masahiro Morita
    Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Mol Cell Biol 27:4980-90. 2007
    ..Thus, CCR4b may be involved in various cellular events that include cell proliferation...
  17. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
    ..The changes in the ER lumen in turn position Ire1 kinase domains in the cytoplasm optimally for autophosphorylation to initiate the UPR...
  18. pmc T2 Family ribonucleases: ancient enzymes with diverse roles
    Natalie Luhtala
    Cancer Biology Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ 85721 0106, USA
    Trends Biochem Sci 35:253-9. 2010
    b>Ribonucleases of the T2 family are found in the genomes of protozoans, plants, bacteria, animals and viruses...
  19. pmc mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes
    Isabelle Behm-Ansmant
    European Molecular Biology Laboratory EMBL, Heidelberg, Germany
    Genes Dev 20:1885-98. 2006
    ..Our findings indicate that GW182 links the miRNA pathway to mRNA degradation by interacting with AGO1 and promoting decay of at least a subset of miRNA targets...
  20. ncbi Multifunctional deadenylase complexes diversify mRNA control
    Aaron C Goldstrohm
    Department of Biochemistry, University of Wisconsin Madison, Wisconsin 53706, USA
    Nat Rev Mol Cell Biol 9:337-44. 2008
    ..We propose that the recruitment of multifunctional deadenylase complexes provides unique opportunities to control mRNAs and that the heterogeneity of the deadenylase complexes is exploited to control translation and mRNA stability...
  21. ncbi Identification of the pollen determinant of S-RNase-mediated self-incompatibility
    Paja Sijacic
    Integrative Biosciences Graduate Degree Program, The Pennsylvania State University, 403 Althouse Laboratory, University Park, Pennsylvania 16802, USA
    Nature 429:302-5. 2004
    ..We conclude that PiSLF encodes the pollen self-incompatibility determinant...
  22. pmc Flavonol activation defines an unanticipated ligand-binding site in the kinase-RNase domain of IRE1
    R Luke Wiseman
    Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 38:291-304. 2010
    ....
  23. ncbi mRNA processing by RNases J1 and J2 affects Bacillus subtilis gene expression on a global scale
    Ulrike Mäder
    Interfaculty Institute for Genetics and Functional Genomics, Department for Functional Genomics, Ernst Moritz Arndt University Greifswald, Walther Rathenau Str 49A, D 17489 Greifswald, Germany
    Mol Microbiol 70:183-96. 2008
    b>Ribonucleases J1 and J2 of Bacillus subtilis are evolutionarily conserved enzymes combining an endoribonucleolytic and a 5'-3' exoribonucleolytic activity in a single polypeptide...
  24. ncbi RNase 7, a novel innate immune defense antimicrobial protein of healthy human skin
    Jurgen Harder
    Clinical Research Unit, Department of Dermatology, University Hospital Kiel, Schittenhelmstrasse 7, D 24105 Kiel, Germany
    J Biol Chem 277:46779-84. 2002
    ..This is the first report demonstrating RNases as a novel class of epithelial inducible antimicrobial proteins, which may play an important role in the innate immune defense system of human epithelia...
  25. ncbi An F-box gene linked to the self-incompatibility (S) locus of Antirrhinum is expressed specifically in pollen and tapetum
    Zhao Lai
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing
    Plant Mol Biol 50:29-42. 2002
    ..S locus product in self-incompatible species from the Solanaceae, Scrophulariaceae and Rosaceae is a class of ribonucleases called S RNases...
  26. ncbi Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism
    B K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 34:1094-108. 1999
    ..6% of their poly(A) tails. Cloning and sequencing of cDNAs derived from lpp, 23S and 16S rRNA revealed that, during exponential growth, C and U residues were polymerized into poly(A) tails in a transcript-dependent manner...
  27. ncbi Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome
    Joseph A Newman
    Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, UK
    J Mol Biol 416:121-36. 2012
    ..Taken together, these data provide valuable insights into the structure and dynamics of the RNA degradosome, a fascinating and complex macromolecular assembly that links RNA degradation with central carbon metabolism...
  28. pmc The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not complex contribute to the prevention of cell death and senescence
    Saloni Mittal
    School of Pharmacy and Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Mol Biol Cell 22:748-58. 2011
    ....
  29. pmc Purification and cloning of cytotoxic ribonucleases from Rana catesbeiana (bullfrog)
    Y D Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Nucleic Acids Res 28:4097-104. 2000
    b>Ribonucleases with antitumor activity are mainly found in the oocytes and embryos of frogs, but the role of these ribonucleases in frog development is not clear...
  30. ncbi Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract
    John D Spencer
    Pediatric Nephrology Fellowship Program, Nationwide Children s Hospital, The Ohio State University, Columbus, Ohio, USA
    Kidney Int 80:174-80. 2011
    ..Thus, RNase 7 is expressed in the human kidney and urinary tract and it may have an important antimicrobial role in maintaining tract sterility...
  31. ncbi The cutting crew - ribonucleases are key players in the control of plastid gene expression
    Rhea Stoppel
    Biozentrum der Ludwig Maximilians Universität, Plant Molecular Biology Botany, Großhaderner Str 2, 82152 Planegg Martinsried, Germany
    J Exp Bot 63:1663-73. 2012
    ..regulation is achieved mainly by post-transcriptional control mechanisms mediated by various nucleus-encoded ribonucleases. More than 180 ribonucleases are annotated in Arabidopsis, but only 17 are predicted to localize to the ..
  32. ncbi The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability
    Kentaro Ito
    Division of Oncology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Protein Cell 2:755-63. 2011
    ..We conclude that CNOT1 contributes to cell viability by securing the activity of the CCR4-NOT deadenylase...
  33. ncbi A bridge crosses the active-site canyon of the Epstein-Barr virus nuclease with DNase and RNase activities
    Marlyse Buisson
    UMI UJF EMBL CNRS, Grenoble, France
    J Mol Biol 391:717-28. 2009
    ..As the DNA strand that is subject to cleavage is likely to make a sharp turn in front of the bridge, endonuclease activity on single-stranded DNA stretches appears to be possible, explaining the cleavage of circular substrates...
  34. ncbi PUF proteins bind Pop2p to regulate messenger RNAs
    Aaron C Goldstrohm
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, Wisconsin 53706, USA
    Nat Struct Mol Biol 13:533-9. 2006
    ..We suggest that the PUF-Pop2p interaction underlies regulated deadenylation, mRNA decay and repression by PUF proteins...
  35. pmc Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation
    Claudia Temme
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, 06099 Halle, Germany
    RNA 16:1356-70. 2010
    ..Nocturnin may also be involved in mRNA deadenylation, whereas there is no evidence for a similar role of Angel and 3635...
  36. pmc Specific roles for the Ccr4-Not complex subunits in expression of the genome
    Nowel Azzouz
    Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva 4, Switzerland
    RNA 15:377-83. 2009
    ..From the study of only two different growth conditions, revealing an impact of the Ccr4-Not complex on more than 85% of all studied genes, we can infer that the Ccr4-Not complex is important for expression of most of the yeast genome...
  37. pmc CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells
    Xianghua Piao
    State Key Laboratory of Molecular Biology and Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Mol Cell Biol 30:1486-94. 2010
    ....
  38. ncbi Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families
    N Ohtani
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan
    Biochemistry 38:605-18. 1999
    ..coli RNases HII and HI, respectively. We propose that Mn2+-dependent RNase HII is universally present in various organisms and Mg2+-dependent RNase HIII, which may have evolved from RNase HII, functions as a substitute for RNase HI...
  39. pmc The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation
    Akhmed Aslam
    The School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Mol Biol Cell 20:3840-50. 2009
    ....
  40. ncbi Enhanced expression and secretion of antimicrobial peptides in atopic dermatitis and after superficial skin injury
    Jurgen Harder
    Department of Dermatology, Allergology, and Venerology, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany
    J Invest Dermatol 130:1355-64. 2010
    ..Thus, a disturbed skin barrier may trigger AMP induction in AD and psoriasis. The functional role of AMP in AD, especially with regard to the control of S. aureus colonization, needs further analysis...
  41. pmc A complex containing the CCR4 and CAF1 proteins is involved in mRNA deadenylation in Drosophila
    Claudia Temme
    Institut fur Biochemie, Martin Luther Universitat Halle Wittenberg, Halle, Germany
    EMBO J 23:2862-71. 2004
    ..The data show that the CCR4-NOT complex is conserved in Drosophila melanogaster and plays a role in general and regulated mRNA deadenylation...
  42. pmc Loss of pollen-S function in two self-compatible selections of Prunus avium is associated with deletion/mutation of an S haplotype-specific F-box gene
    Tineke Sonneveld
    Plant Science Division, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom
    Plant Cell 17:37-51. 2005
    ....
  43. ncbi Bacillus subtilis ribonucleases J1 and J2 form a complex with altered enzyme behaviour
    Nathalie Mathy
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Paris, France
    Mol Microbiol 75:489-98. 2010
    b>Ribonucleases J1 and J2 are recently discovered enzymes with dual 5'-to-3' exoribonucleolytic/endoribonucleolytic activity that plays a key role in the maturation and degradation of Bacillus subtilis RNAs...
  44. ncbi Refined crystal structures of native human angiogenin and two active site variants: implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth
    D D Leonidas
    Department of Biology and Biochemistry, University of Bath, Claverton Down, BA2 7AY, UK
    J Mol Biol 285:1209-33. 1999
    ..The H13A structure shows some changes beyond the ribonucleolytic site, but sites involved in cell-binding and nuclear translocation are essentially unaffected by the amino acid replacement...
  45. pmc Crystal structure of the human CNOT6L nuclease domain reveals strict poly(A) substrate specificity
    Hui Wang
    Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, PR China
    EMBO J 29:2566-76. 2010
    ..The resulting structures suggest a molecular deadenylase mechanism involving a pentacovalent phosphate transition...
  46. pmc X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex
    Stéphane Thore
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 4:1150-5. 2003
    ..This first structure of a nuclease involved in the 3'-5' deadenylation of mRNA in yeast provides information for the understanding of the mechanism by which the Ccr4-Not complex achieves its functions...
  47. pmc Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits
    T K Albert
    Laboratory for Physiological Chemistry and Centre for Biomedical Genetics, Utrecht University, PO Box 80042, 3508 TA Utrecht, The Netherlands
    Nucleic Acids Res 28:809-17. 2000
    ..Conserved interactions of this factor with both NOT and CCR4 proteins and co-immunoprecipitation experiments suggest that CALIF is a bona fide component of the human CCR4-NOT complex...
  48. pmc Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot
    Francesca L Short
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    Proc Natl Acad Sci U S A 110:E241-9. 2013
    ....
  49. ncbi Regulation of neutrophil and eosinophil secondary granule gene expression by transcription factors C/EBP epsilon and PU.1
    Adrian F Gombart
    Division of Hematology Oncology, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA
    Blood 101:3265-73. 2003
    ..Furthermore, it indicates the importance of PU.1 in the cooperative activation of eosinophil granule genes...
  50. ncbi Identification of a S-ribonuclease-binding protein in Petunia hybrida
    T L Sims
    Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, 60115 2861, USA
    Plant Mol Biol 47:771-83. 2001
    ..Together, these results suggest that PhSBPI may be a candidate for the recently proposed general inhibitor (RI) of self-incompatibility ribonucleases.
  51. pmc The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility
    Hong Qiao
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
    Plant Cell 16:2307-22. 2004
    ..inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S(2) controls the pollen function of S-RNase-based self-incompatibility...
  52. ncbi The eight human "canonical" ribonucleases: molecular diversity, catalytic properties, and special biological actions of the enzyme proteins
    Salvatore Sorrentino
    Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy
    FEBS Lett 584:2194-200. 2010
    Human ribonucleases (RNases) are members of a large superfamily of rapidly evolving homologous proteins. Upon completion of the human genome, eight catalytically active RNases (numbered 1-8) were identified...
  53. ncbi Exoribonuclease R interacts with endoribonuclease E and an RNA helicase in the psychrotrophic bacterium Pseudomonas syringae Lz4W
    Rajyaguru Ichchhashankar Purusharth
    Institut für Mikrobiologie und Molekularbiologie der Justus Liebig Universität Giessen, Heinrich Buff Ring 26 32, 35392 Giessen, Germany
    J Biol Chem 280:14572-8. 2005
    ..The physical association of RNase E with an exoribonuclease and an RNA helicase apparently is a common theme in the composition of bacterial RNA-degrading complexes...
  54. pmc Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae
    Mercedes E Arana
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 11:933-41. 2012
    ..A large number of DEGs encode nucleases, helicases and genes involved in response to dsRNA viruses, observations that could be relevant to the nucleic acid species that elicit an innate immune response in RNase H2-defective humans...
  55. pmc The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex
    Alain Pierre Petit
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    Nucleic Acids Res 40:11058-72. 2012
    ..Our findings shed light on the assembly of the CCR4-NOT complex and provide the basis for dissecting the role of the NOT module in mRNA deadenylation...
  56. ncbi Mycobacterium smegmatis RNase J is a 5'-3' exo-/endoribonuclease and both RNase J and RNase E are involved in ribosomal RNA maturation
    Valerio Taverniti
    Department of Biomolecular Sciences and Biotechnology, University of Milano, Via Celoria 26, 20133 Milano, Italy
    Mol Microbiol 82:1260-76. 2011
    ..coli and in B. subtilis. Studying organisms containing different combinations of key ribonucleases can thus significantly broaden our view of the possible strategies that exist to direct RNA metabolism.
  57. pmc The virion host shutoff protein of herpes simplex virus 1 blocks the replication-independent activation of NF-κB in dendritic cells in the absence of type I interferon signaling
    Christopher R Cotter
    Department of Microbiology and Immunology Institute, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 85:12662-72. 2011
    ....
  58. pmc The herpes simplex virus 1 vhs protein enhances translation of viral true late mRNAs and virus production in a cell type-dependent manner
    Bianca Dauber
    Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
    J Virol 85:5363-73. 2011
    ..The varied permissivity of cell lines to vhs-null infection may stem from differences in the resilience of the translation machinery or the ability to control the accumulation of mRNAs...
  59. pmc A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systems
    Dapeng Zhang
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 39:4532-52. 2011
    ....
  60. ncbi Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes
    Mai Minamikawa
    Chiba University, Matsudo, Japan
    Plant Mol Biol 74:143-54. 2010
    ..by a complex S locus that encodes separate proteins for pistil and pollen specificities, extracellular ribonucleases (S-RNases) and F-box proteins SFB/SLF, respectively...
  61. ncbi Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in Rosaceae
    Hidenori Sassa
    Graduate School of Horticulture, Chiba University, Matsudo, Chiba, 271 8510, Japan
    Sex Plant Reprod 23:39-43. 2010
    ..This review focuses on the potential differences in pollen-part function between subfamilies of Rosaceae, Maloideae, and Prunoideae, and discusses implications for the mechanistic divergence of the S-RNase-based SI...
  62. pmc Specificity of RppH-dependent RNA degradation in Bacillus subtilis
    Ping Kun Hsieh
    Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 110:8864-9. 2013
    ..By contrast, a parallel pathway for 5'-end-dependent RNA degradation in that species appears to involve an alternative phosphate-removing enzyme that is relatively insensitive to sequence variation at the first three positions...
  63. ncbi A new self-compatibility haplotype in the sweet cherry 'Kronio', S5', attributable to a pollen-part mutation in the SFB gene
    Annalisa Marchese
    East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK
    J Exp Bot 58:4347-56. 2007
    ..Analysis of some other Sicilian cherries with these primers indicated that S(5)' is also present in the Sicilian cultivar 'Maiolina a Rappu', and this proved to be self-compatible...
  64. pmc Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins
    Guillaume Moissiard
    Institut de Biologie Moleculaire des Plantes, Strasbourg Cedex, France
    RNA 13:1268-78. 2007
    ..Finally, we show that target cleavage by a primary small RNA and 3-->5' transitivity can be genetically uncoupled, and we provide in vivo evidence supporting a key role for priming in this specific reaction...
  65. ncbi A pathogenesis related protein, AhPR10 from peanut: an insight of its mode of antifungal activity
    Pooja Chadha
    Comparative Genomics Unit, Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110 007, India
    Planta 225:213-22. 2006
    ..Energy and temperature dependent internalization of AhPR10 into sensitive fungal hyphae indicate that internalization of the protein occurs through active uptake...
  66. ncbi Ribonucleases as a novel pro-apoptotic anticancer strategy: review of the preclinical and clinical data for ranpirnase
    John Costanzi
    Lone Star Oncology, Austin, Texas, USA
    Cancer Invest 23:643-50. 2005
    Cytotoxic ribonucleases (RNases), such as ranpiranase, represent a novel mechanism-based approach to anticancer therapy...
  67. pmc Archaeal β-CASP ribonucleases of the aCPSF1 family are orthologs of the eukaryal CPSF-73 factor
    Duy Khanh Phung
    Centre National de la Recherche Scientifique, LMGM and Université de Toulouse, UPS, F 31062 Toulouse, France
    Nucleic Acids Res 41:1091-103. 2013
    ..J and eukaryal cleavage and polyadenylation specificity factor (CPSF-73) are members of the β-CASP family of ribonucleases involved in mRNA processing and degradation...
  68. ncbi Rapid evolution of a unique family of primate ribonuclease genes
    H F Rosenberg
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 10:219-23. 1995
    ..These results suggest that both EDN and ECP may be responding to unusual evolutionary constraints, which has prompted a reexamination of their physiologic function...
  69. pmc Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family
    H F Rosenberg
    Department of Medicine, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215
    Proc Natl Acad Sci U S A 86:4460-4. 1989
    ..in position 7, which was previously unidentified in the amino acid sequences of EDN or the urinary and liver ribonucleases. Both EDN and the related granule protein, eosinophil cationic protein, have ribonucleolytic activity; ..
  70. pmc Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease
    G J Gleich
    Proc Natl Acad Sci U S A 83:3146-50. 1986
    ..15 micrograms when injected into the cisterna magna; the proteins were comparable in their activities. These results indicate that EDN and ECP are related proteins and suggest that they derived from genes associated with the RNase family...
  71. pmc RNS2, a conserved member of the RNase T2 family, is necessary for ribosomal RNA decay in plants
    Melissa S Hillwig
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 108:1093-8. 2011
    ..We propose that RNS2 is part of a process that degrades rRNA to recycle its components. This process appears to be conserved in all eukaryotes...
  72. pmc Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus attenuation
    G Meyers
    Department of Immunology, Federal Research Centre for Virus Diseases of Animals, D 72001 Tübingen, Germany
    J Virol 73:10224-35. 1999
    ..Analyses of reisolated viruses gave no indication for the presence of revertants in the infected animals...
  73. pmc RNase 7 contributes to the cutaneous defense against Enterococcus faecium
    Bente Köten
    Department of Dermatology, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany
    PLoS ONE 4:e6424. 2009
    ..Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system against E. faecium...
  74. ncbi Identification of the substrate binding site in the N-terminal TBP-like domain of RNase H3
    Seiko Miyashita
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan
    FEBS Lett 585:2313-7. 2011
    ..This domain may greatly change conformation upon substrate binding...
  75. ncbi Ribonucleases of different origins with a wide spectrum of medicinal applications
    Evandro Fei Fang
    School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong
    Biochim Biophys Acta 1815:65-74. 2011
    b>Ribonucleases (RNases) are a type of nucleases that catalyze the degradation of RNA into smaller components. They exist in a wide range of life forms from prokaryotes to eukaryotes...
  76. pmc Cytoplasmic deadenylation: regulation of mRNA fate
    Katrin Wiederhold
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Biochem Soc Trans 38:1531-6. 2010
    ..In the present paper, we discuss mRNA deadenylation in eukaryotes, focusing on the main deadenylase, the Ccr4-Not complex, including its composition, regulation and functional roles...
  77. pmc The major component in schistosome eggs responsible for conditioning dendritic cells for Th2 polarization is a T2 ribonuclease (omega-1)
    Svenja Steinfelder
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:1681-90. 2009
    ..Based on this evidence, we hypothesize that S. mansoni omega-1 acts by limiting the interaction of DCs with CD4(+) T lymphocytes, thereby lowering the strength of the activation signal delivered...
  78. pmc Structural and transcriptional analysis of the self-incompatibility locus of almond: identification of a pollen-expressed F-box gene with haplotype-specific polymorphism
    Koichiro Ushijima
    Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Plant Cell 15:771-81. 2003
    ..These features are consistent with the hypothesis that SFB is the pollen S gene. This hypothesis predicts the involvement of the ubiquitin/26S proteasome proteolytic pathway in the RNase-based gametophytic self-incompatibility system...
  79. pmc Functional identification of toxin-antitoxin molecules from Helicobacter pylori 26695 and structural elucidation of the molecular interactions
    Kyung Doo Han
    Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Kwanak gu, Seoul 151 742, Korea
    J Biol Chem 286:4842-53. 2011
    ..Taken together, it is concluded that the HP0894-HP0895 protein couple is a TA system in H. pylori, where HP0894 is a toxin with an RNase function, whereas HP0895 is an antitoxin functioning by binding to both the toxin and DNA...
  80. ncbi Darwin's foundation for investigating self-incompatibility and the progress toward a physiological model for S-RNase-based SI
    Bruce McClure
    Division of Biochemistry, Interdisciplinary Plant Group, Christopher S Bond Life Sciences Center, University of Missouri, Columbia, MO 65211 7310, USA
    J Exp Bot 60:1069-81. 2009
    ..Current research is aimed at understanding how the mutual actions of these S-gene products function in the physiological context of pollen tube growth...
  81. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  82. pmc The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets
    Eric Huntzinger
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Nucleic Acids Res 41:978-94. 2013
    ..melanogaster and human cells indicate that miRNA-mediated translational repression and degradation are mechanistically linked and are triggered through the interactions of GW182 proteins with PABP and deadenylases...
  83. pmc The Caenorhabditis elegans GW182 protein AIN-1 interacts with PAB-1 and subunits of the PAN2-PAN3 and CCR4-NOT deadenylase complexes
    Duygu Kuzuoglu-Öztürk
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Nucleic Acids Res 40:5651-65. 2012
    ....
  84. pmc The wobble nucleotide-excising anticodon nuclease RloC is governed by the zinc-hook and DNA-dependent ATPase of its Rad50-like region
    Daniel Klaiman
    Department of Biochemistry, Tel Aviv University, Tel Aviv 69978, Israel
    Nucleic Acids Res 40:8568-78. 2012
    ..We also discuss RloC's possible evolution from a PrrC-like ancestor...
  85. pmc NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs
    Atsushi Suzuki
    Yokohama National University, Yokohama, Kanagawa 240 8501, Japan
    Proc Natl Acad Sci U S A 107:3594-9. 2010
    ..We propose from our current findings that NANOS2-interacting RNAs may be recruited to P-bodies and degraded by the enzymes contained therein through NANOS2-mediated deadenylation...
  86. ncbi A tale of two continents: Baker's rule and the maintenance of self-incompatibility in Lycium (Solanaceae)
    Jill S Miller
    Department of Biology, Amherst College, Amherst, MA 01002, USA
    Evolution 62:1052-65. 2008
    ..The present data thus provide a dramatic exception to Baker's rule, in addition to supporting a key assumption of the Miller and Venable (2000) model, namely that self-incompatibility is associated with diploidy and cosexuality...
  87. ncbi Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
    Angelique Deleris
    Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Science 313:68-71. 2006
    ..These findings define a molecular framework for studying antiviral silencing and defense in plants...
  88. pmc MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment
    Francesco P Marchese
    Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London W6 8LH, United Kingdom
    J Biol Chem 285:27590-600. 2010
    ..Cyclooxygenase-2 mRNA stability is increased in CAF1-depleted cells in which it is no longer p38 MAPK/MK2-regulated...
  89. ncbi Collaborative non-self recognition system in S-RNase-based self-incompatibility
    Ken ichi Kubo
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 0192, Japan
    Science 330:796-9. 2010
    ..It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin-26S-proteasome ..
  90. ncbi Gametophytic self-incompatibility inhibits pollen tube growth using different mechanisms
    Noni V E Franklin-Tong
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK B15 2TT
    Trends Plant Sci 8:598-605. 2003
    ..Because the limited data suggest the independent evolution of these two GSI systems, it would be interesting to explore other GSI systems to determine the extent of the mechanistic diversity...
  91. pmc The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme
    Anette Thyssen Jonstrup
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark
    Nucleic Acids Res 35:3153-64. 2007
    ....
  92. ncbi Site-directed mutagenesis of histidine-13 and histidine-114 of human angiogenin. Alanine derivatives inhibit angiogenin-induced angiogenesis
    R Shapiro
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 28:7401-8. 1989
    ..The capacity of the H13A and H114A derivatives to compete with angiogenin in the chorioallantoic membrane assay suggests several additional features of the biological mode of action of this protein...
  93. ncbi Constitutive expression of the antimicrobial peptide RNase 7 is associated with Staphylococcus aureus infection of the skin
    Philipp Zanger
    Institut fur Tropenmedizin, Eberhard Karls Universitat, 72074 Tubingen, Germany
    J Infect Dis 200:1907-15. 2009
    ..This study aimed to clarify whether their baseline expression influences the propensity of healthy individuals to develop S. aureus-positive skin infections...
  94. ncbi Stressing out over tRNA cleavage
    Debrah M Thompson
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Cell 138:215-9. 2009
    A conserved response to stress involves endonucleolytic cleavage of cytoplasmic transfer RNAs (tRNAs) by ribonucleases that are normally secreted or sequestered...
  95. pmc The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae
    Debrah M Thompson
    Department of Molecular and Cellular Biology, and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 185:43-50. 2009
    ..Thus, detection of Rny1p, and possibly its orthologues, in the cytosol may be a conserved mechanism for assessing cellular damage and determining cell survival, analogous to the role of cytochrome c as a marker for mitochondrial damage...
  96. ncbi Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex
    J Chen
    Department of Biochemistry and Molecular Biology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA
    J Mol Biol 314:683-94. 2001
    ..The distinct location of CAF40 and CAF130 and the evolutionary conservation of CAF40 implicate them in novel roles in the function of the CCR4-NOT complex...
  97. pmc Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly
    Qing Li
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cell 134:244-55. 2008
    ..These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106...
  98. ncbi Angiogenin is a cytotoxic, tRNA-specific ribonuclease in the RNase A superfamily
    S K Saxena
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 267:21982-6. 1992
    ..One of these homologues purified from human eosinophils, eosinophil-derived neurotoxin, nonspecifically degrades oocyte RNA similar to RNase A and is also cytotoxic at very low concentrations...
  99. ncbi Antimicrobial skin peptides and proteins
    J M Schroder
    Department of Dermatology, University Hospital Schleswig Holstein, Campus Kiel, Schittenhelmstr 7, 24105 Kiel, Germany
    Cell Mol Life Sci 63:469-86. 2006
    ..Such mediators might be ideal 'immune stimulants' to induce only the innate antimicrobial skin effector molecules without causing inflammation...
  100. ncbi Self-compatibility of 'Katy' apricot (Prunus armeniaca L.) is associated with pollen-part mutations
    Jun Wu
    College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, People s Republic of China
    Sex Plant Reprod 24:23-35. 2011
    ..These findings support a hypothesis that modifying factors other than the S-locus are required for GSI in apricot...
  101. ncbi Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species
    Ryutaro Tao
    Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, 606 8502, Japan
    Plant Mol Biol 63:109-23. 2007
    ..We discuss the significance of SC pollen part mutation in peach with special reference to possible differences in the SI mechanisms between Prunus and Solanaceae...

Research Grants63

  1. Roles for microRNA-122 in hepatitis C virus RNA amplification
    Peter Sarnow; Fiscal Year: 2013
    ..2 will test the hypothesis that miR-122 protects the 5'end sequences of the HCV RNA from degradation by ribonucleases or RNA modification enzymes, or aids in the avoidance of activation of double-stranded RNA sensors such as the ..
  2. Enterovirus RNA Translation and Replication
    David J Barton; Fiscal Year: 2013
    ..The success of the poliovirus eradication campaign may depend upon improved poliovirus vaccines and new antiviral drugs. ..
  3. HORMONAL REGULATION OF MESSENGER RNA STABILITY
    Daniel R Schoenberg; Fiscal Year: 2013
    ....
  4. Murine coronavirus neurovirulence: role of type I interferon response
    Susan R Weiss; Fiscal Year: 2013
    ..Moreover, understanding type I IFN signaling in the CNS may aid in developing or refining therapeutic applications for type I IFNs, which are currently used in treatment of MS and hepatitis C. ..
  5. Genetics and Genomics of the yidC paralogs in Streptococcus mutans
    SARA MARIE RASER PALMER; Fiscal Year: 2013
    ..The data generated will provide essential new information that can be used to design novel and more effective therapies to combat dental caries and other bacterial infections. ..
  6. miRNAs and epidermal langerhans cell development and function
    Qing Sheng Mi; Fiscal Year: 2011
    ....
  7. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2013
    ..abstract_text> ..
  8. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
    ..RNase III also is a component of RNA editosomes in trypanosome mitochondria. dsRNA processing by bacterial ribonucleases III regulates cellular and phage gene expression, plasmid replication, antibiotic production, and virulence ..
  9. Enzymology of RNA Processing
    Carol A Fierke; Fiscal Year: 2013
    ....
  10. Ribozymes for studies and treatment of cytomegalovirus infections
    Fenyong Liu; Fiscal Year: 2012
    ..Our study will facilitate the development of novel macromolecules that can be used as research tools and therapeutic agents for studies and treatment of infections of human viruses including HCMV. ..
  11. Regulation of RNA Surveillance
    MILES FROME WILKINSON; Fiscal Year: 2012
    ..This proposal addresses newly discovered mechanisms responsible for regulating NMD that are particularly relevant for understanding normal neurological function and the genetic basis for mental retardation. ..
  12. NMR Studies of Structures and Dynamics of Proteins
    Arthur G Palmer; Fiscal Year: 2013
    ....
  13. Global analysis of mRNA decay in Bacillus subtilis
    David H Bechhofer; Fiscal Year: 2013
    ..More recently, two new classes of bacterial ribonucleases, the RNase J and RNase Y families, were discovered in B. subtilis. One of the B...
  14. Role of the antiviral ribonuclease, RNase-L, in the host antibacterial response
    Bret A Hassel; Fiscal Year: 2012
    ..The proposed studies will provide the first information on the role of RNase-L as a novel mediator of antibacterial activity, and will evaluate the antibacterial activity of RNase-L activators as a new class of therapeutic agent. ..
  15. Molecular Activities of Angiogenin in the Nucleus
    KIMBERLY ANNE DICKSON; Fiscal Year: 2012
    ..The proposed research will impart a better understanding of the molecular actions of ANG in diseased and normal cells, laying the scientific groundwork for future development of drugs designed to target ANG. ..
  16. Neuronal-Specific Splicing of CaV1.2 L-type Calcium Channels
    Kristin Webster; Fiscal Year: 2013
    ..I hypothesize that the e1b isoform will more efficiently induce CREB phosphorylation. These experiments will advance our understanding of how neuronal specific isoforms of CaV1.2 contribute to essential neuronal functions. ..
  17. Mechanism of angiogenin-induced angiogenesis
    Guo fu Hu; Fiscal Year: 2013
    ..These results will provide a comprehensive understanding of the function and mechanism of ANG in angiogenesis and in cancer progression. ..
  18. Alternaria and ribonucleases in Th2-type immunity
    Hirohito Kita; Fiscal Year: 2013
    ..We also hypothesize that exogenous and endogenous ribonucleases (RNases) are involved in development of pathologic Th2 cells...
  19. Understanding microRNA Biogenesis and Function
    Phillip D Zamore; Fiscal Year: 2013
    ..Thus, the proposed studies promise to contribute to a better understanding of and therapy for human diseases. ..
  20. Proteins in RNA-Based Enzymes
    Andrey S Krasilnikov; Fiscal Year: 2012
    ....
  21. Molecular Mechanisms of Gene Silencing by RNAi
    Richard W Carthew; Fiscal Year: 2013
    ..Moreover, small RNAs are used experimentally to probe gene function and are being commercially developed as disease therapeutics, making basic understanding of these molecules an important biomedical research goal. ..
  22. Mechanism of CsrA-Mediated Global Control
    Tony Romeo; Fiscal Year: 2013
    ..on CsrA-mediated regulation of two stress-response sigma factors ([unreadable]S and [unreadable]E), and two ribonucleases that participate in bulk mRNA turnover (RNase E and PNPase)...
  23. RNAi-Mediated Heterochromatin Assembly
    Danesh Moazed; Fiscal Year: 2013
    ..The ultimate goal of these studies is a complete molecular understanding of gene silencing. ..
  24. Structural studies of RNase P
    ALFONSO MONDRAGON; Fiscal Year: 2013
    ..RNase P is a promising target for the development of new chemotherapeutics as the specificity of RNase P can be altered to create molecules that degrade target RNA molecules. ..
  25. Novel antivirals targeting the RNase H activity of HIV reverse transcriptase
    Zhengqiang Wang; Fiscal Year: 2013
    ..Our multidisciplinary approach will lead to new inhibitors of HIV that will be effective against both wild- type and drug-resistant viral strains. ..
  26. Enterovirus RNA Translation and Replication
    David Barton; Fiscal Year: 2009
    ..These insights will be important for designing safe and effective vaccines for PV and other group C enteroviruses as well as antiviral drug therapies for many positive-strand RNA viruses. ..
  27. Ionizing Radiation Control of Inflammatory Cytokine mRNA Stability and Expression
    Robert Schneider; Fiscal Year: 2013
    ..abstract_text> ..
  28. REGULATION OF MRNA PROCESSING
    Phillip A Sharp; Fiscal Year: 2012
    ..These studies will both increase our knowledge of mechanisms controlling alternative splicing and as well the factors that control CD44 activity in diseases such as cancer. ..
  29. Molecular Regulation of CC Chemokine in Atherosclerosis and Aging
    Raymond L Yung; Fiscal Year: 2010
    ..A better understanding of the role of aging plays in modulating the inflammatory response in coronary artery disease will improve the care of, and potentially lead to novel therapies for, the rapidly aging US population. ..
  30. Biochemical Mechanisms of Drug Resistance in HIV RT
    Yanbin Zhang; Fiscal Year: 2012
    ....
  31. Adherence Activated Monocyte Genes
    Gary Brewer; Fiscal Year: 2009
    ..assembly of a large mRNA-protein complex that targets ARE-mRNAs for rapid destruction by cytoplasmic messenger ribonucleases. Moreover, these processes are subject to regulatory control in response to changes in AUF1 phosphorylation ..
  32. Discovery of Novel HIV-1 Reverse Transcriptase Inhibitors by Fragment Screening
    ANGELA MCKOY; Fiscal Year: 2013
    ..The fragment screening approach allows for efficient search of chemical space and should lead to the identification of novel scaffolds for inhibiting HIV-1 RNH activity at both the active site and allosteric pockets. ..
  33. NNRTI induced conformational changes in HIV-1 RT
    NICOLAS P SLUIS-CREMER; Fiscal Year: 2012
    ....
  34. RNases and RNA Metabolism in Bacteria
    Murray P Deutscher; Fiscal Year: 2013
    ..While considerable information has accumulated about the ribonucleases (RNases) essential to RNA metabolism, much remains to be learned about their complete number, about their ..
  35. Novel mechanisms of HIV resistance to RTIs
    NICOLAS P SLUIS-CREMER; Fiscal Year: 2012
    ....
  36. The Control of Gene Expression by Eukaryotic Ribonucleases
    Guillaume F Chanfreau; Fiscal Year: 2013
    ..Our studies of the turnover of unspliced and mis-spliced RNAs will shed light on the mechanisms by which RNA degradation controls the expression of genes mutated in the context of a large number of genetic diseases. ..
  37. Biochemical Studies of Drosophila RNA-induced Silencing Complex
    Qinghua Liu; Fiscal Year: 2010
    ....
  38. A systems approach to understand translation and sRNA-mediated gene regulation in
    JARED MICHAEL SCHRADER; Fiscal Year: 2013
    ..With this broad approach, I will determine how global mRNA translation is regulated to ensure the dynamic changes in gene expression that drive cell cycle progression in Caulobacter. ..
  39. Comparative Analysis of RNA Helicases in E. coli
    Chaitanya Jain; Fiscal Year: 2012
    ..Advanced knowledge of such RNA helicases could also be helpful to design novel antibiotics that target conserved DEAD-box domains in pathogenic organisms. ..
  40. GENETIC ANALYSIS OF RICKETTSIA PROWAZEKII
    David Wood; Fiscal Year: 2010
    ..Modulation of Rho and its correlation to these changes will be: assessed. ..
  41. Monitoring mechanisms in mammalian ribosome biogenesis
    Dimitri G Pestov; Fiscal Year: 2013
    ..Because the framework of preribosomes, like the ribosome itself, is made of RNA, ribonucleases play a key role in dismantling defective ribosome precursors...
  42. Structure and Function of RNAi-related Nucleases
    Jennifer A Doudna; Fiscal Year: 2013
    ..Two specialized cytoplasmic ribonucleases function at either end of this pathway: the Dicer endonuclease catalyzes length-specific double-strand RNA ..
  43. Ribonuclease 7: Antimicrobial Activity in the Human Kidney and Urinary Tract
    John David Spencer; Fiscal Year: 2013
    ..Successful completion of these proposed aims and educational plan will help develop the needed expertise to systematically evaluate AMPs, like RNase 7, in the human urinary tract. ..
  44. Structure and function of RNase P
    Michael E Harris; Fiscal Year: 2013
    ..Additionally we are developing methods to detect ion binding at individual phosphates using isotope-edited Raman. ..
  45. Improving Efficacy of RNase Cancer Therapy by Pharmacokinetics
    Laura E Strong; Fiscal Year: 2010
    ..The EVade Ribonucleases are recombinant variants of mammalian enzymes that are effective in mouse xenograft models of multiple cancer ..
  46. Analysis of E. coli ribonucleases and RNA metabolism
    Sidney R Kushner; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Although numerous ribonucleases have been identified over the past 40 years, most have been associated primarily with the biological reaction that was used for their identification...
  47. Acylhydrazone inhibitors of HIV-1 ribonuclease H
    MICHAEL A contact PARNIAK; Fiscal Year: 2010
    ..New drugs are essential. This project will explore compounds directed at a novel HIV target, ribonuclease H, with the goal of developing new drugs that will be active against existing drug resistant HIV. ..
  48. Chromatin Degradation During Apoptosis
    Ding Xue; Fiscal Year: 2013
    ..These studies should reveal the novel mechanism that controls the specificity and function switch of the dicer nuclease and new signaling mechanisms and players that control the release of the mitochondrial apoptogenic factors. ..
  49. Mildly acidic tumor microenvironment for diagnostic and therapeutic nanomedicine
    JENNICA L KRANKEL; Fiscal Year: 2013
    ..e. proteins and peptides) as anti-cancer therapeutics. ..
  50. Structure and Function in Catalytic RNP Assembly
    Mark Foster; Fiscal Year: 2009
    ..With recent efforts to make use of the substrate selectivity of RNase P to engineer customized ribozymes for use in gene therapy, insights into the molecular basis for the enzyme's function is clearly essential. ..
  51. Angiogenin-induced RNA cleavage in cancer
    Paul J Anderson; Fiscal Year: 2013
    ..The proposed research is innovative because it focuses on the direct target of ANG, its RNA substrates, and attempts to determine how tRNA cleavage promotes tumorigenesis. ..
  52. RIBONUCLEASES IN CANCER CHEMOTHERAPY
    Ronald T Raines; Fiscal Year: 2013
    ..Hence, ONC has the potential to be the harbinger of a new class of cancer chemotherapeutics based on ribonucleases. Ribonuclease- based chemotherapeutic agents are unrelated to extant chemotherapeutics and thus represent a ..
  53. ANTIVIRAL EFFECTS OF EOSINOPHILS IN THE LUNGS
    David Jacoby; Fiscal Year: 2007
    ..Our studies suggest that this is due to metabolism of bromide by eosinophil peroxidase (EPO). Eosinophil ribonucleases (eosinophil derived neurotoxin (EDN) and eosinophil cationic protein (ECP)) may also participate...
  54. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    David H Bechhofer; Fiscal Year: 2010
    ..in 1 or more 3'-to-5'exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  55. Ribonuclease III mechanism of double-strand RNA cleavage
    ALLEN NICHOLSON; Fiscal Year: 2003
    ..coil RNase Ill, but will also provide mechanistic insight on RNase Ill orthologue function in eukaryotic RNA processing and degradation pathways, including RNAi. ..
  56. Epididymis-specific proteins for male contraception
    FRANK FRENCH; Fiscal Year: 2004
    ..One, ESC461 is related to the eosinophil ribonucleases A2 and A3 which have antimicrobial activity...
  57. THE RIBONUCLEASES INVOLVED IN RNA INTERFERENCE
    Brenda L Bass; Fiscal Year: 2010
    ..The latter will allow the first characterization of endo-siRNA precursors. The proposed research will advance our understanding of Dicer, an enzyme that is absolutely essential for life. ..
  58. Micro RNA Expression and Cancer
    Thomas Schmittgen; Fiscal Year: 2005
    ..Assays developed with this research may help to identify the function of a new class of genes in humans. ..
  59. STRUCTURAL ANALYSIS OF SYNTHETICALLY ENGINEERED RNASES
    Brian Edwards; Fiscal Year: 1993
    ..analysis will continue to be applied to single crystals of a series of semisynthetic bovine pancreatic ribonucleases which exhibit altered catalytic efficiency or substrate specificity, with the intention of delineating further ..
  60. EVOLUTION OF THE RIBONUCLEASE SUPERFAMILY
    Steven Benner; Fiscal Year: 2001
    ....
  61. RELEASE AND HOST INACTIVATION OF SSRNA BACTERIOPHAGES
    JEANNE POINDEXTER; Fiscal Year: 1992
    ..polypeptides that stimulate nearby uninfected cells to activate and, in some instances, to secrete ribonucleases that confer on those cells a temporary anti-viral state...
  62. Novel RNase-based Immunotoxin for CD74-positive B-cell Malignancies
    Chien Hsing Chang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  63. Conformational Dynamics and inhibitor responses of HIV-1 RT RNase H in solution
    Rieko Ishima; Fiscal Year: 2013
    ....