phosphoric monoester hydrolases

Summary

Summary: A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.

Top Publications

  1. ncbi PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer
    J Li
    Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168 Street, New York, NY 10032, USA
    Science 275:1943-7. 1997
  2. ncbi The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate
    T Maehama
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    J Biol Chem 273:13375-8. 1998
  3. ncbi Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN
    V Stambolic
    Amgen Institute, and Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Cell 95:29-39. 1998
  4. ncbi Pten is essential for embryonic development and tumour suppression
    A Di Cristofano
    Department of Human Genetics, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, New York, NY 10021, USA
    Nat Genet 19:348-55. 1998
  5. ncbi Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association
    J O Lee
    Cellular Biochemistry and Biophysics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 99:323-34. 1999
  6. pmc Inositol phosphatase SHIP1 is a primary target of miR-155
    Ryan M O'Connell
    Division of Biology, California Institute of Technology, 330 Braun, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:7113-8. 2009
  7. pmc Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling
    Christina Gewinner
    Division of Signal Transduction, Beth Israel Deaconess Medical Center, Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 16:115-25. 2009
  8. ncbi High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice
    A Suzuki
    Amgen Institute Ontario Cancer Institute Department of Medical Biophysics and Immunology University of Toronto Toronto, Ontario, Canada M5G 2C1
    Curr Biol 8:1169-78. 1998
  9. ncbi Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN
    M Tamura
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Science 280:1614-7. 1998
  10. ncbi Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers
    P A Steck
    Department of Neuro Oncology, University of Texas, M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 15:356-62. 1997

Research Grants

  1. Biomarkers and Chemoprevention of Endometrial Cancer
    George Mutter; Fiscal Year: 2006
  2. Hormonal modulation of latent endometrial precancers
    George Mutter; Fiscal Year: 2008
  3. Signaling by IgG receptors leading to phagocytosis
    KENNETH COGGESHALL; Fiscal Year: 2006
  4. Molecular Neurodevelopment: An In Vivo 31P-1H MRSI Study
    JAY PETTEGREW; Fiscal Year: 2006
  5. Role of Integrins in Pulmonary Fibrosis
    Eric White; Fiscal Year: 2006
  6. VACCINIA VIRUS DNA TOPOISOMERASE
    Stewart Shuman; Fiscal Year: 2006
  7. TUMOR CELL BIOLOGY OF THE FMS ONCOGENE PROTEINS
    LARRY ROHRSCHNEIDER; Fiscal Year: 2005
  8. Cellular Signaling Mechanisms in Macrophage Activation.
    Joseph Cuschieri; Fiscal Year: 2007
  9. IN VIVO 31P-1H MRSI/MRI OF CHRONIC SCHIZOPHRENIA
    JAY PETTEGREW; Fiscal Year: 2003
  10. IN VIVO 31P-1H MRSI AND MRI BRAIN STUDIES OF NICOTINE
    JAY PETTEGREW; Fiscal Year: 2004

Detail Information

Publications294 found, 100 shown here

  1. ncbi PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer
    J Li
    Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168 Street, New York, NY 10032, USA
    Science 275:1943-7. 1997
    ..These homologies suggest that PTEN may suppress tumor cell growth by antagonizing protein tyrosine kinases and may regulate tumor cell invasion and metastasis through interactions at focal adhesions...
  2. ncbi The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate
    T Maehama
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    J Biol Chem 273:13375-8. 1998
    ..As expected, the C124S mutant of PTEN was incapable of catalyzing dephosphorylation of PtdIns(3,4,5)P3 consistent with the mechanism observed in protein-tyrosine phosphatase-catalyzed reactions...
  3. ncbi Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN
    V Stambolic
    Amgen Institute, and Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Cell 95:29-39. 1998
    ..Our results show that PTEN may exert its role as a tumor suppressor by negatively regulating the PI3'K/PKB/Akt signaling pathway...
  4. ncbi Pten is essential for embryonic development and tumour suppression
    A Di Cristofano
    Department of Human Genetics, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, New York, NY 10021, USA
    Nat Genet 19:348-55. 1998
    ..These results support the notion that PTEN haploinsufficiency plays a causal role in CD, LDD and BZS pathogenesis, and demonstrate that Pten is a tumour suppressor essential for embryonic development...
  5. ncbi Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association
    J O Lee
    Cellular Biochemistry and Biophysics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 99:323-34. 1999
    ..The phosphatase and C2 domains associate across an extensive interface, suggesting that the C2 domain may serve to productively position the catalytic domain on the membrane...
  6. pmc Inositol phosphatase SHIP1 is a primary target of miR-155
    Ryan M O'Connell
    Division of Biology, California Institute of Technology, 330 Braun, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:7113-8. 2009
    ..Our study unveils a molecular link between miR-155 and SHIP1 and provides evidence that repression of SHIP1 is an important component of miR-155 biology...
  7. pmc Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling
    Christina Gewinner
    Division of Signal Transduction, Beth Israel Deaconess Medical Center, Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 16:115-25. 2009
    ....
  8. ncbi High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice
    A Suzuki
    Amgen Institute Ontario Cancer Institute Department of Medical Biophysics and Immunology University of Toronto Toronto, Ontario, Canada M5G 2C1
    Curr Biol 8:1169-78. 1998
    ..PTEN is widely expressed, and Cowden disease consistently affects various organ systems, suggesting that the PTEN protein must have an important, although as yet poorly understood, function in cellular physiology...
  9. ncbi Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN
    M Tamura
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Science 280:1614-7. 1998
    ..Overexpression of FAK partially antagonized the effects of PTEN. Thus, PTEN phosphatase may function as a tumor suppressor by negatively regulating cell interactions with the extracellular matrix...
  10. ncbi Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers
    P A Steck
    Department of Neuro Oncology, University of Texas, M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 15:356-62. 1997
    ..Our results identify a strong candidate tumour suppressor gene at chromosome 10q23.3, whose loss of function appears to be associated with the oncogenesis of multiple human cancers...
  11. ncbi Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin
    Ryusuke Niwa
    Department of Molecular Genetics, The Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Cell 108:233-46. 2002
    ..Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo...
  12. pmc New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway
    L C Cantley
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:4240-5. 1999
    ..PTEN appears to negatively control the phosphoinositide 3-kinase signaling pathway for regulation of cell growth and survival by dephosphorylating the 3 position of phosphoinositides...
  13. pmc The lipid phosphatase activity of PTEN is critical for its tumor supressor function
    M P Myers
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724 2208, USA
    Proc Natl Acad Sci U S A 95:13513-8. 1998
    ....
  14. pmc Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:377-82. 2006
    ....
  15. pmc Redox regulation of PI 3-kinase signalling via inactivation of PTEN
    Nick R Leslie
    Division of Cell Signalling, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    EMBO J 22:5501-10. 2003
    ..These results may have significant implications for the many cellular processes in which PtdIns(3,4,5)P(3) and oxidants are produced concurrently...
  16. pmc Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway
    S Ramaswamy
    Department of Adult Oncology, Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:2110-5. 1999
    ..Finally, tumor cells lacking PTEN contain high levels of activated Akt, suggesting that PTEN is necessary for the appropriate regulation of the phosphatidylinositol 3-kinase/Akt pathway...
  17. ncbi Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion
    D Raucher
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 100:221-8. 2000
    ..Our study suggests that plasma membrane PIP2 controls dynamic membrane functions and cell shape by locally increasing and decreasing the adhesion between the actin-based cortical cytoskeleton and the plasma membrane...
  18. pmc Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems
    K Podsypanina
    Departments of Pathology and Medicine, College of Physicians and Surgeons, Columbia University, 630 W 168th Street, P and S 14 453, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 96:1563-8. 1999
    ..Taken together, these data suggest that PTEN is a regulator of apoptosis and proliferation that behaves as a "landscaper" tumor suppressor in the gut and a "gatekeeper" tumor suppressor in other organs...
  19. pmc Negative control in two-component signal transduction by transmitter phosphatase activity
    TuAnh Ngoc Huynh
    Food Science Graduate Group Department of Microbiology, University of California, Davis, California, USA
    Mol Microbiol 82:275-86. 2011
    ....
  20. ncbi A PTEN-like phosphatase with a novel substrate specificity
    David J Pagliarini
    Department of Pharmacology, University of California, San Diego, La Jolla, California 92093 0721, USA
    J Biol Chem 279:38590-6. 2004
    ..The unique in vitro catalytic activity and detailed biochemical and kinetic analyses reported here will be of great value in our continued efforts to identify in vivo substrate(s) for this highly conserved phosphatase...
  21. ncbi The histidine phosphatase superfamily: structure and function
    Daniel J Rigden
    School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
    Biochem J 409:333-48. 2008
    ....
  22. pmc Src homology 2 domain-containing inositol-5-phosphatase and CCAAT enhancer-binding protein beta are targeted by miR-155 in B cells of Emicro-MiR-155 transgenic mice
    Stefan Costinean
    Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
    Blood 114:1374-82. 2009
    ..We hypothesize that miR-155, by down-modulating Ship and C/EBPbeta, initiates a chain of events that leads to the accumulation of large pre-B cells and acute lymphoblastic leukemia/high-grade lymphoma...
  23. ncbi Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers
    G L Mutter
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Natl Cancer Inst 92:924-30. 2000
    ..Testing the hypothesis that altered PTEN function precedes the appearance of endometrial adenocarcinoma has been difficult, however, partly because of uncertainties in precancer diagnosis...
  24. ncbi A new class of phosphotransferases phosphorylated on an aspartate residue in an amino-terminal DXDX(T/V) motif
    J F Collet
    Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology and Catholic University of Louvain, B 1200 Brussels, Belgium
    J Biol Chem 273:14107-12. 1998
    ..In conclusion, phosphomannomutase and L-3-phosphoserine phosphatase belong to a new phosphotransferase family with an amino-terminal DXDX(T/V) motif that serves as an intermediate phosphoryl acceptor...
  25. pmc Validating GSK3 as an in vivo target of lithium action
    W Timothy O'Brien
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Biochem Soc Trans 37:1133-8. 2009
    ..These observations, from several independent laboratories, support a central role for GSK3 in mediating behavioural responses to lithium...
  26. ncbi Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis
    K Sakumi
    Medical Institute of Bioregulation, Faculty of Science, Kyushu University, Fukuoka, Japan
    J Biol Chem 268:23524-30. 1993
    ..In such cells, the frequency of spontaneous mutation was greatly reduced. We propose that the human 8-oxo-dGTPase protects genetic information from the untoward effects of endogenous oxygen radicals...
  27. pmc Inositol polyphosphate 4-phosphatase II regulates PI3K/Akt signaling and is lost in human basal-like breast cancers
    Clare G Fedele
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Proc Natl Acad Sci U S A 107:22231-6. 2010
    ....
  28. pmc Insights on the evolution of trehalose biosynthesis
    Nelson Avonce
    Centro de Investigación en Biotecnología UAEM, Av Universidad 1001, Col Chamilpa, Cuernavaca 62210, Mexico
    BMC Evol Biol 6:109. 2006
    ..However, in recent years it has been shown that although higher plants do not accumulate trehalose at significant levels they have actively transcribed genes encoding the corresponding biosynthetic enzymes...
  29. ncbi Essential role of phosphoinositide metabolism in synaptic vesicle recycling
    O Cremona
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell 99:179-88. 1999
    ..These results provide genetic evidence for a crucial role of phosphoinositide metabolism in synaptic vesicle recycling...
  30. pmc Onco-miR-155 targets SHIP1 to promote TNFalpha-dependent growth of B cell lymphomas
    Irene M Pedersen
    Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA
    EMBO Mol Med 1:288-95. 2009
    ....
  31. pmc A glutamate switch controls voltage-sensitive phosphatase function
    Lijun Liu
    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 19:633-41. 2012
    ..Together, the data suggest that functional coupling between the gating loop and the linker forms the heart of the regulatory mechanism that controls voltage-dependent enzyme activation...
  32. ncbi Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis
    Daphné Dambournet
    Institut Pasteur, Membrane Traffic and Cell Division Lab 25 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nat Cell Biol 13:981-8. 2011
    ..They also reveal an unexpected role for the phosphatase OCRL in cell division and shed new light on the pleiotropic phenotypes associated with Lowe disease...
  33. ncbi T cell-specific loss of Pten leads to defects in central and peripheral tolerance
    A Suzuki
    Department of Molecular and Cellular Biology, Osaka University, 565 0871, Osaka, Japan
    Immunity 14:523-34. 2001
    ..Peripheral tolerance to SEB is also impaired in Pten(flox/-) mice. PTEN is thus an important regulator of T cell homeostasis and self-tolerance...
  34. pmc Inositol 5-phosphatases: insights from the Lowe syndrome protein OCRL
    Michelle Pirruccello
    Department of Cell Biology, HHMI and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Trends Biochem Sci 37:134-43. 2012
    ..Additionally, structural analysis of missense mutations in the catalytic domain of OCRL provides insight into the phenotypic heterogeneity observed in Lowe syndrome and Dent disease...
  35. pmc Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4
    Brandon E Kremer
    Center for Cell Signaling, Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Mol Biol Cell 16:4648-59. 2005
    ..These data identify a novel molecular function for septins in mammalian cells: the modulation of microtubule dynamics through interaction with MAP4...
  36. pmc Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase
    Niroshika Keppetipola
    Molecular Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10021, USA
    RNA 12:73-82. 2006
    ..Deletion analysis defined an autonomous phosphatase domain, CthPnkp-(171-424)...
  37. ncbi TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta
    D M Li
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Cancer Res 57:2124-9. 1997
    ..Our functional studies of the TEP1 protein suggest that its tumor suppressor function may associate with its intrinsic protein tyrosine phosphatase activity and its cytoplasmic localization...
  38. ncbi Src homology 2 domain-containing inositol-5-phosphatase 1 (SHIP1) negatively regulates TLR4-mediated LPS response primarily through a phosphatase activity- and PI-3K-independent mechanism
    Huazhang An
    Institute of Immunology, Second Military Medical University, Shanghai, People s Republic of China
    Blood 105:4685-92. 2005
    ....
  39. pmc Decreased expression and androgen regulation of the tumor suppressor gene INPP4B in prostate cancer
    Myles C Hodgson
    Florida International University, Miami, Florida 33199, USA
    Cancer Res 71:572-82. 2011
    ..Our findings reinforce the concept that patients undergoing androgen ablation may benefit from Akt-targeting therapies...
  40. ncbi Mutations of the human PTEN gene
    D Bonneau
    Department of Medical Genetics, University of Poitiers, Poitiers, France
    Hum Mutat 16:109-22. 2000
    ..A mutagenesis by insertion-deletion in repetitive elements is however specifically observed in endometrial carcinomas...
  41. ncbi Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis
    K M Yamada
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
    J Cell Sci 114:2375-82. 2001
    ....
  42. pmc Structural basis of response regulator dephosphorylation by Rap phosphatases
    Vijay Parashar
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, New Jersey, United States of America
    PLoS Biol 9:e1000589. 2011
    ....
  43. ncbi The role of the inositol polyphosphate 5-phosphatases in cellular function and human disease
    Lisa M Ooms
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Biochem J 419:29-49. 2009
    ..Emerging studies have implicated their loss or gain of function in human disease...
  44. ncbi High incidence of breast and endometrial neoplasia resembling human Cowden syndrome in pten+/- mice
    V Stambolic
    Amgen Research Institute and Ontario Cancer Institute, Toronto, Canada
    Cancer Res 60:3605-11. 2000
    ..Taken together, our results establish pten+/- mice as an excellent animal model system for the investigation of PTEN-related hamartoma syndromes, as well as the role of PTEN in breast and endometrial carcinogenesis...
  45. ncbi p150Ship, a signal transduction molecule with inositol polyphosphate-5-phosphatase activity
    M N Lioubin
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Genes Dev 10:1084-95. 1996
    ..Ectopic expression of p150 in fibroblasts does not inhibit growth. This novel protein, p150(ship) (SH2-containing inositol phosphatase), identifies a component of a new growth factor-receptor signaling pathway in hematopoietic cells...
  46. pmc PTEN/MMAC1/TEP1 suppresses the tumorigenicity and induces G1 cell cycle arrest in human glioblastoma cells
    D M Li
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:15406-11. 1998
    ....
  47. pmc Ultrasensitivity in multisite phosphorylation of membrane-anchored proteins
    Omer Dushek
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    Biophys J 100:1189-97. 2011
    ..We propose a new role, to our knowledge, for multisite membrane-anchored proteins, discuss experiments that can be used to probe the model, and relate our findings to previous theoretical work...
  48. pmc TPIP: a novel phosphoinositide 3-phosphatase
    S M Walker
    Division of Cell Signalling, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 360:277-83. 2001
    ..We suggest that TPIP alpha plays a role in regulating phosphoinositide signalling on the endoplasmic reticulum, and might also represent a tumour suppressor and functional homologue of PTEN in some tissues...
  49. pmc The src homology 2-containing inositol phosphatase (SHIP) is the gatekeeper of mast cell degranulation
    M Huber
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada V5Z 1L3
    Proc Natl Acad Sci U S A 95:11330-5. 1998
    ..These results show the critical role that SHIP plays in setting the threshold for degranulation and that SHIP directly modulates a "positive-acting" receptor...
  50. pmc The 145-kDa protein induced to associate with Shc by multiple cytokines is an inositol tetraphosphate and phosphatidylinositol 3,4,5-triphosphate 5-phosphatase
    J E Damen
    The Terry Fox Laboratory, British Columbia Cancer Agency, University of British Columbia, Vancouver, Canada
    Proc Natl Acad Sci U S A 93:1689-93. 1996
    ..This novel signal transduction intermediate may serve to modulate both Ras and inositol signaling pathways. Based on its properties, we suggest the 145-kDa protein be called SHIP for SH2-containing inositol phosphatase...
  51. ncbi Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage
    A Jilani
    Molecular Oncology Group, Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec H3T 1E2
    J Biol Chem 274:24176-86. 1999
    ..PNKP gene function restored termini suitable for DNA polymerase, consistent with in vivo removal of 3'-phosphate groups, facilitating DNA repair...
  52. ncbi Oxidative damage to RNA and expression patterns of MTH1 in the hippocampi of senescence-accelerated SAMP8 mice and Alzheimer's disease patients
    Xiao Ning Song
    The Key Laboratory of Geriatrics, Beijing Hospital, Beijing Institute of Geriatrics, Ministry of Health, No 1, DaHua Road, Dong Dan, 100730, Beijing, People s Republic of China
    Neurochem Res 36:1558-65. 2011
    ..These results suggest that MTH1 deficiency might be a causative factor for aging and age-related disorders...
  53. ncbi Significance of glucose intolerance and SHIP2 expression in hepatocellular carcinoma patients with HCV infection
    Shuji Sumie
    Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Fukuoka 830 0011, Japan
    Oncol Rep 18:545-52. 2007
    ..In conclusion, this study is the first to demonstrate the significance of glucose intolerance in prognosis of male HCC patients and down-regulation of SHIP2 expression in HCC...
  54. ncbi Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability
    John Dowdle
    School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Plant J 52:673-89. 2007
    ..The GDP-L-galactose phosphorylase step may therefore play an important role in controlling ascorbate biosynthesis...
  55. pmc Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1- and 4'-phosphatase activities
    Stephen R Coats
    Department of Periodontics, School of Dentistry, University of Washington, 1959 NE Pacific St, Seattle, WA 98195 7444, USA
    Cell Microbiol 11:1587-99. 2009
    ..gingivalis lipid A activity from TLR4 evasive to TLR4 suppressive, potentially altering critical interactions between this bacterium, the local microbial community and the host innate immune system...
  56. ncbi MutT homolog-1 attenuates oxidative DNA damage and delays photoreceptor cell death in inherited retinal degeneration
    Yusuke Murakami
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Am J Pathol 181:1378-86. 2012
    ....
  57. pmc The phosphoinositide phosphatase Sjl2 is recruited to cortical actin patches in the control of vesicle formation and fission during endocytosis
    Christopher J Stefan
    Department of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla, CA 92093 0068, USA
    Mol Cell Biol 25:2910-23. 2005
    ..Together, our studies suggest that PI metabolism by the synaptojanin-like proteins coordinately directs actin dynamics and membrane invagination, in part by regulation of Sla2...
  58. pmc Mammalian enzymes for preventing transcriptional errors caused by oxidative damage
    Toru Ishibashi
    Biomolecular Engineering Research Institute Suita, Osaka 565 0874, Japan
    Nucleic Acids Res 33:3779-84. 2005
    ..These results indicate that the elimination of 8-oxoGua-containing ribonucleotides from the precursor pool is important to ensure accurate protein synthesis and that both NUDT5 and MTH1 are involved in this process in human cells...
  59. pmc The inositol polyphosphate 5-phosphatase, PIPP, Is a novel regulator of phosphoinositide 3-kinase-dependent neurite elongation
    Lisa M Ooms
    Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Victoria, Australia
    Mol Biol Cell 17:607-22. 2006
    ..PIPP therefore inhibits PI3-kinase-dependent neurite elongation in PC12 cells, via regulation of the spatial distribution of phospho-Ser473-Akt and phospho-Ser9-GSK-3beta signaling...
  60. ncbi Substrate specificity and membrane topology of Escherichia coli PgpB, an undecaprenyl pyrophosphate phosphatase
    Thierry Touze
    Universite Paris Sud, UMR 8619, Institut de Biochimie et Biophysique Moléculaire et Cellulaire and CNRS, Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619, 91405 Orsay Cedex, France
    J Biol Chem 283:16573-83. 2008
    ..Furthermore, a topological analysis demonstrated that PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location...
  61. ncbi An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress
    Daisuke Yoshimura
    Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 278:37965-73. 2003
    ..Human MTH1 thus protects cells from H2O2-induced cell dysfunction and death by hydrolyzing oxidized purine nucleotides including 8-oxo-dGTP and 2-OH-dATP, and these alterations may be partly attributed to a mitochondrial dysfunction...
  62. ncbi The Cytophaga hutchinsonii ChTPSP: First characterized bifunctional TPS-TPP protein as putative ancestor of all eukaryotic trehalose biosynthesis proteins
    Nelson Avonce
    Department of Molecular Microbiology, VIB, Heverlee, Belgium
    Mol Biol Evol 27:359-69. 2010
    ....
  63. pmc The yeast synaptojanin-like proteins control the cellular distribution of phosphatidylinositol (4,5)-bisphosphate
    Christopher J Stefan
    Division of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla, California 92093 0068, USA
    Mol Biol Cell 13:542-57. 2002
    ..Taken together, our results demonstrate that the yeast synaptojanins control the localization of PtdIns(4,5)P(2) in vivo and provide further evidence for the compartmentalization of different PI species...
  64. ncbi Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome
    D Liaw
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Nat Genet 16:64-7. 1997
    ..Thus, PTEN appears to behave as a tumour suppressor gene in the germline. Our data also imply that PTEN may play a role in organizing the relationship of different cell types within an organ during development...
  65. pmc The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus
    Shannon McNulty
    Microbiology and Molecular Genetics Graduate Program, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Virol 85:7402-10. 2011
    ..Thus, the inhibitory effects of A34 on virus release are mediated by SHIP2. Together, these data suggest that SHIP2 and A34 may act as gatekeepers to regulate dissemination of poxviruses when environmental conditions are conducive...
  66. pmc Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence
    William J Ratzan
    Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Cell Physiol 226:2740-6. 2011
    ....
  67. ncbi Contribution of hMTH1 to the maintenance of 8-oxoguanine levels in lung DNA of non-small-cell lung cancer patients
    Elzbieta Speina
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    J Natl Cancer Inst 97:384-95. 2005
    ....
  68. pmc Exome sequencing identifies INPPL1 mutations as a cause of opsismodysplasia
    Celine Huber
    Département de génétique, Unité INSERM U781, Université Paris Descartes Sorbonne Paris Cité, Fondation Imagine, Hopital Necker Enfants Malades, Paris 75015, France
    Am J Hum Genet 92:144-9. 2013
    ..Our finding of INPPL1 mutations in OPS, a severe spondylodysplastic dysplasia with major growth plate disorganization, supports a key and specific role of this enzyme in endochondral ossification...
  69. pmc Dynamics of two Phosphorelays controlling cell cycle progression in Caulobacter crescentus
    Y Erin Chen
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 191:7417-29. 2009
    ..More generally, our results emphasize how the bifunctional nature of histidine kinases can help switch cells between mutually exclusive states...
  70. ncbi SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases
    S Guo
    Departments of Pharmacology and Cancer Biology and of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 274:12990-5. 1999
    ..Additionally, the tethering of PPIPase and 5-phosphatase activities indicate a novel mechanism by which concerted phosphoinositide hydrolysis participates in membrane trafficking...
  71. ncbi SHIP2 and its involvement in various diseases
    Akira Suwa
    Astellas Pharma, Inc, Pharmacology Research Labs, Drug Discovery Research, 21, Miyukigaoka, Tsukuba shi, Ibaraki 305 8585, Japan
    Expert Opin Ther Targets 14:727-37. 2010
    ..The functional upregulation of SHIP2 results in signaling blockade, leading to related disorders...
  72. pmc Reprogramming the tRNA-splicing activity of a bacterial RNA repair enzyme
    Niroshika Keppetipola
    Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Nucleic Acids Res 35:3624-30. 2007
    ..Our findings suggest that RNA repair enzymes can evolve their specificities to suit a particular pathway...
  73. pmc Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase
    T Tsuzuki
    Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 98:11456-61. 2001
    ..The MTH1-deficient mouse will provide a useful model for investigating the role of the MTH1 protein in normal conditions and under oxidative stress...
  74. pmc Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis
    Ji Zhang
    Department of Pharmacology, University of California San Diego, La Jolla, CA 92093, USA
    Cell Metab 13:690-700. 2011
    ..Our study identifies PTPMT1 as the mammalian PGP phosphatase and points to its role as a regulator of cardiolipin biosynthesis...
  75. pmc Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain
    Alina D Gutu
    Department of Biology, Indiana University Bloomington, Bloomington, IN 47405, USA
    J Bacteriol 192:2346-58. 2010
    ..In addition, these WalK(Spn) DHp domain and DeltaPAS mutations led to attenuation of virulence in a murine pneumonia model...
  76. ncbi Autophagy differentially controls plant basal immunity to biotrophic and necrotrophic pathogens
    Heike D Lenz
    Center for Plant Molecular Biology, Eberhard Karls University of Tubingen, 72076 Tubingen, Germany
    Plant J 66:818-30. 2011
    ..We infer that the way in which autophagy contributes to plant immunity to different pathogens is mechanistically diverse, and thus resembles the complex role of this process in animal innate immunity...
  77. pmc The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain
    N Ringstad
    Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 94:8569-74. 1997
    ..These findings underscore the important role of SH3-mediated interactions in synaptic vesicle recycling...
  78. ncbi SHIP negatively regulates IgE + antigen-induced IL-6 production in mast cells by inhibiting NF-kappa B activity
    Janet Kalesnikoff
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    J Immunol 168:4737-46. 2002
    ....
  79. ncbi SH2-containing inositol phosphatase (SHIP-1) transiently translocates to raft domains and modulates CD16-mediated cytotoxicity in human NK cells
    Ricciarda Galandrini
    Department of Experimental Medicine and Pathology, Istituto Pasteur Fondazione Cenci Bolognetti, University La Sapienza, Rome, Italy
    Blood 100:4581-9. 2002
    ..These data demonstrate that CD16 engagement on NK cells induces membrane targeting and activation of SHIP-1, which acts as negative regulator of ADCC function...
  80. ncbi Human pyridoxal phosphatase. Molecular cloning, functional expression, and tissue distribution
    Young Min Jang
    Department of Biochemistry, Kyungpook National University, Taegu 702 701, Korea
    J Biol Chem 278:50040-6. 2003
    ..1 kb was detected in all human tissues examined and was highly abundant in the brain. Obtaining the molecular properties for the human PLP phosphatase may provide new direction for investigating metabolic pathway involving vitamin B6...
  81. pmc Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase
    Niroshika Keppetipola
    Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Nucleic Acids Res 35:7721-32. 2007
    ....
  82. ncbi The Lowe syndrome protein OCRL1 is involved in primary cilia assembly
    Brian G Coon
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Hum Mol Genet 21:1835-47. 2012
    ..This conceptual change in our understanding of the disease etiology may provide an alternative avenue for the development of therapies...
  83. ncbi IMPA1 is essential for embryonic development and lithium-like pilocarpine sensitivity
    Kim Cryns
    Research and Early Development Europe, Johnson and Johnson Pharmaceutical Research and Development, Beerse, Belgium
    Neuropsychopharmacology 33:674-84. 2008
    ..In conclusion the IMPA1-/- mouse represents a novel model to study inositol homeostasis, and indicates that genetic inactivation of IMPA1 can mimic some actions of lithium...
  84. ncbi Biophysical characterization of the fluorescent protein voltage probe VSFP2.3 based on the voltage-sensing domain of Ci-VSP
    Alicia Lundby
    Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako City, Saitama, 351 0198, Japan
    Eur Biophys J 39:1625-35. 2010
    ..The data presented give us insights into the voltage-sensing mechanism of Ci-VSP, which will allow us to further improve the sensitivity and kinetics of the family of VSFP proteins...
  85. pmc Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications
    Krishnamurthy N Rao
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Protein Sci 15:1735-44. 2006
    ..We have confirmed that T6PP is a trehalose phosphatase from amino acid sequence, three-dimensional structure, and biochemical assays...
  86. pmc Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP
    Susy C Kohout
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Chem Biol 6:369-75. 2010
    ..We propose that Ci-VSP activity is self-limited because its decrease of PI(4,5)P2 levels decouples the VSD from the enzyme...
  87. ncbi PI(3,4,5)P3 and PI(3,4)P2 levels correlate with PKB/akt phosphorylation at Thr308 and Ser473, respectively; PI(3,4)P2 levels determine PKB activity
    Kewei Ma
    Deparment of Medicine, University of British Columbia and Vancouver Coastal Health Research Institute, Vancouver, BC, Canada
    Cell Signal 20:684-94. 2008
    ..We conclude that PI(3,4,5)P3 and PI(3,4)P2 have distinct roles in determining PKB phosphorylation and activity. Thus, when investigating PI3K-PKB pathways, the importance of both lipids must be considered...
  88. ncbi Biochemical characterization of aspartyl phosphate phosphatase interaction with a phosphorylated response regulator and its inhibition by a pentapeptide
    Shu Ishikawa
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:20483-9. 2002
    ..The structural organization of Rap phosphatases in tetratricopeptide repeats provides insights on the mechanisms of RapA interaction with its substrate and its inhibitor...
  89. ncbi The PtdIns(3,4)P(2) phosphatase INPP4A is a suppressor of excitotoxic neuronal death
    Junko Sasaki
    Department of Medical Biology, Akita University Graduate School of Medicine, Akita 010 8543, Japan
    Nature 465:497-501. 2010
    ..The discovery of a direct link between PtdIns(3,4)P(2) metabolism and the regulation of neurodegeneration and involuntary movements may aid the development of new approaches for the treatment of neurodegenerative disorders...
  90. pmc Targeted disruption of SHIP leads to hemopoietic perturbations, lung pathology, and a shortened life span
    C D Helgason
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada
    Genes Dev 12:1610-20. 1998
    ..Thus, homozygous disruption of SHIP establishes the crucial role of this molecule in modulating cytokine signaling within the hemopoietic system and provides a powerful model for further delineating its function...
  91. pmc MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening
    Hesso Farhan
    Biozentrum, Universitat Basel, 4056 Basel, Switzerland
    J Cell Biol 189:997-1011. 2010
    ..Our analysis reveals an unanticipated complexity of kinase/phosphatase-mediated regulation of the secretory pathway, uncovering a link between growth factor signaling and ER export...
  92. pmc OCRL1 function in renal epithelial membrane traffic
    Shanshan Cui
    Renal Electrolyte Division, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol Renal Physiol 298:F335-45. 2010
    ....
  93. pmc INPP4B: the new kid on the PI3K block
    Irina U Agoulnik
    Herbert Wertheim College of Medicine, Miami, FL, USA Email
    Oncotarget 2:321-8. 2011
    ..We will review its structure and function, crosstalk with androgen receptor signaling, and regulation of INPP4B expression, as well as existing data about its role in cancer...
  94. ncbi Structure, function, and biology of SHIP proteins
    L R Rohrschneider
    Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington 98109 1024, USA
    Genes Dev 14:505-20. 2000
  95. pmc Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development
    Stephane Lefebvre
    Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom
    Plant Physiol 138:451-60. 2005
    ..This work has also shown that it is not necessary to bypass the normal regulatory control of SBPase, exerted by conditions in the stroma, to achieve improvements in carbon fixation...
  96. ncbi A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5
    Michele Yeo
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0650, USA
    J Biol Chem 278:26078-85. 2003
    ..Accordingly, SCP1 may play a role in the regulation of gene expression, possibly by controlling the transition from initiation/capping to processive transcript elongation...
  97. ncbi Impaired Fas response and autoimmunity in Pten+/- mice
    A Di Cristofano
    Department of Human Genetics Molecular Biology Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10021, USA
    Science 285:2122-5. 1999
    ..These results indicate that Pten is an essential mediator of the Fas response and a repressor of autoimmunity and thus implicate the PI 3-kinase/Akt pathway in Fas-mediated apoptosis...
  98. pmc Vascular endothelium as a contributor of plasma sphingosine 1-phosphate
    Krishnan Venkataraman
    Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Circ Res 102:669-76. 2008
    ..These data suggest that the vascular endothelium, in addition to the hematopoietic system, is a major contributor of plasma S1P...
  99. ncbi Germline mutations in PTEN are present in Bannayan-Zonana syndrome
    D J Marsh
    Nat Genet 16:333-4. 1997
  100. ncbi Phosphoinositides in constitutive membrane traffic
    Michael G Roth
    Dept of Biochemistry, Univ of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9038, USA
    Physiol Rev 84:699-730. 2004
    ..Although the lipid kinases and phosphatases that function in constitutive membrane traffic are beginning to be identified, their regulation is poorly understood...
  101. ncbi Novel mechanism of PTEN regulation by its phosphatidylinositol 4,5-bisphosphate binding motif is critical for chemotaxis
    Miho Iijima
    Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 279:16606-13. 2004
    ....

Research Grants70

  1. Biomarkers and Chemoprevention of Endometrial Cancer
    George Mutter; Fiscal Year: 2006
    ..If successful, we will be able to rapidly implement a rational and effective strategy for early detection and chemoprevention of endometrial carcinoma. ..
  2. Hormonal modulation of latent endometrial precancers
    George Mutter; Fiscal Year: 2008
    ..If our hypothesis is confirmed, this project will establish biomarker-detectable preclinical disease as a surrogate marker for risk surveillance, and a potential target for cancer preventative therapies. ..
  3. Signaling by IgG receptors leading to phagocytosis
    KENNETH COGGESHALL; Fiscal Year: 2006
    ..The results will provide a better understanding of molecular process involved in clearance of infectious agents in humans. ..
  4. Molecular Neurodevelopment: An In Vivo 31P-1H MRSI Study
    JAY PETTEGREW; Fiscal Year: 2006
    ..Longitudinal 31P-1H MRSI and single voxel 1H MRS follow-up of the subjects yearly for 4 years after entry into study will be used to generate neurodevelopmental curves for brain/metabolic measures. ..
  5. Role of Integrins in Pulmonary Fibrosis
    Eric White; Fiscal Year: 2006
    ....
  6. VACCINIA VIRUS DNA TOPOISOMERASE
    Stewart Shuman; Fiscal Year: 2006
    ..5) Biochemical and genetic characterization of the TopIB proteins from Mycobacterium avium, Mycobacterium smegmatis, and Pseudomonas aeurginosa. ..
  7. TUMOR CELL BIOLOGY OF THE FMS ONCOGENE PROTEINS
    LARRY ROHRSCHNEIDER; Fiscal Year: 2005
    ..Overall, the results of these experiments will describe new signaling mechanisms that may be useful for understanding the normal and abnormal development of blood cells and their diseases. ..
  8. Cellular Signaling Mechanisms in Macrophage Activation.
    Joseph Cuschieri; Fiscal Year: 2007
    ..Through an enhanced understanding of these mechanisms it is our goal that potential therapeutic targets may be discovered to regulate the inflammatory response following trauma/hemorrhage and ischemia/reperfusion. ..
  9. IN VIVO 31P-1H MRSI/MRI OF CHRONIC SCHIZOPHRENIA
    JAY PETTEGREW; Fiscal Year: 2003
    ....
  10. IN VIVO 31P-1H MRSI AND MRI BRAIN STUDIES OF NICOTINE
    JAY PETTEGREW; Fiscal Year: 2004
    ..We propose to investigate these aspects of brain metabolism in humans using 31P-1H MRSI. ..
  11. The role of s-SHIP in normal and tumorigenic development of the skin
    LARRY ROHRSCHNEIDER; Fiscal Year: 2007
    ..Relevance: The research in this proposal is aimed at understanding the expression and function of a single protein which may be critical for the development of the epidermis. [unreadable] [unreadable] [unreadable]..
  12. Novel Targets for Treatment of Smallpox
    Stewart Shuman; Fiscal Year: 2003
    ....
  13. Signal Transduction in Enterococci
    Marta Perego; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  14. Molecular Studies of Cognition in Chronic Alcoholism
    Jay W Pettegrew; Fiscal Year: 2010
    ..abstract_text> ..
  15. Protein Aspartate Phosphatases in Development
    Marta Perego; Fiscal Year: 2006
    ..The role of the Rap phosphatases not involved in sporulation will be investigated by microbiological and genetic approaches ..
  16. Neurobiol mechanism/acupuncture therapy/alcoholism
    Jiang Hong Ye; Fiscal Year: 2004
    ..This new knowledge will have a major impact on our understanding of how acupuncture works. The application of this knowledge is expected to improve the effectiveness of acupuncture treatment in alcohol addiction. ..
  17. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2009
    ..A population of subjects will be examined for this relationship. ..
  18. PHOSPHATIDYLINOSITOL 3 KINASE IN B CELL ACTIVATION
    KENNETH COGGESHALL; Fiscal Year: 2003
    ..Such information may reveal novel therapeutic targets in a variety of pathologic conditions and contribute to the etiology of such diseases. ..
  19. SHIP PROTEINS AND SIGNAL TRANSDUCTION
    LARRY ROHRSCHNEIDER; Fiscal Year: 2003
    ..abstract_text> ..
  20. Chemical Complementation Assay for MKP-1 (RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..This proposal will provide sufficient reagents, including cells and DMA transfection vectors, to permit the high throughput screening of 100,000 compounds. ..
  21. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
    ..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
  22. Chemical Complementation Assay for MKP-3 (RMI)
    JOHN LAZO; Fiscal Year: 2005
    ..This proposal will provide sufficient reagents, including cells and DMA transfection vectors, to permit the high throughput screening of 100,000 compounds. ..
  23. QTL mapping for genes regulating apoptosis capacity
    Jianfeng Xu; Fiscal Year: 2010
    ..Our proposal describes a novel and efficient approach to identify apoptosis genes that are critical in cancer susceptibility. ..
  24. Protein Prenyltransferases in Glucose-Stimulated Insulin Secretion
    Anjaneyulu Kowluru; Fiscal Year: 2010
    ....
  25. Phosphatidylinositol Signaling and Human Disease
    Philip W Majerus; Fiscal Year: 2010
    ..In summary we use inositol biochemistry to determine the phenotype vs genotype of disorders of inositol metabolism. ..
  26. Pittsburgh Molecular Libraries Screening Center(RMI)
    JOHN LAZO; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  27. Targeted Therapy of Lyn in Myelodysplastic Syndrome
    SETH JOEL COREY; Fiscal Year: 2010
    ..These studies will provide rationale for a phase II study using Src inhibitors for subsets of MDS patients. ..
  28. FTF and gpCYP7A1 are targets of protein kinases
    Diane Stroup; Fiscal Year: 2006
    ..The data will serve as a basis for further studies using the proteomics techniques of 2D HPLC and SELDI. ..
  29. Intermediate Filaments 2008 Gordon Research Conference
    Thomas Shea; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  30. Association of inflammatory genes and prostate cancer
    Jianfeng Xu; Fiscal Year: 2008
    ..Answers to these questions will significantly improve our knowledge of the role of inflammation in prostate cancer and will guide our efforts in identifying additional prostate cancer genes. ..
  31. Is S-adenosyl Methionine a Nutrition: Genetic Link in AD?
    Thomas Shea; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  32. Regulation of Cx43 by Src and Novel Interacting Proteins
    Alan Lau; Fiscal Year: 2009
    ....
  33. Mapping Prostate Cancer Susceptibility Genes at 8p22-23
    Jianfeng Xu; Fiscal Year: 2007
    ..Successful identification of prostate cancer genes will have a significant impact on understanding the etiology, prevention, diagnosis, and treatment of prostate cancer. ..
  34. H.pylori arginase modulation of ulcers and cancer
    David McGee; Fiscal Year: 2007
    ..pylori arginase in protection from innate defenses as well as roles in ulcer and cancer development and optimization of nitrogen levels in vivo. ..
  35. Phosphoinositide and Inositol Polyphosphate Probes
    Glenn Prestwich; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  36. The Roles of Osteonectin and Osteoactivin in Gliomas
    Jeremy Rich; Fiscal Year: 2007
    ..2) Validate osteonectin and osteoactivin as potential therapeutic targets in glioma invasion. It is hoped that these studies will provide the basis of novel therapeutic interventions for patients with malignant gliomas. ..
  37. INTERACTION OF HELICOBACTER PYLORI WITH PHAGOCYTES
    LEE ANN ALLEN; Fiscal Year: 2003
    ..A complete dissection of this process at the molecular level may lead to novel therapies for treatment of H. pylori infection and reduce the significant morbidity associated with ulcer disease. ..
  38. Indentification of AKT2/aPKC Substrates in Adipocytes
    Prem Sharma; Fiscal Year: 2005
    ..We believe that the findings from the proposed investigations will improve our general understanding of players of insulin resistance and offer potential insight into new therapeutic modalities. ..
  39. Pathway of Spontaneous Mutagenesis in Escherichia coli
    Robert Fowler; Fiscal Year: 2004
    ..Humans possess a number of these specialized DNA polymerases whose activities may be responsible for a significant portion of the human mutational load. ..
  40. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ....
  41. CHANNEL-MEDIATED RBC DEHYDRATION IN SICKLE CELL DISEASE
    Alicia Rivera; Fiscal Year: 2003
    ..It is additionally significant, because it will provide the means for the applicant to establish an independent research career and to become competitive for a tenure-track assistant professional position. ..
  42. INFLAMMATION AND ATROPHY IN PROSTATE CARCINOGENESIS
    Angelo Demarzo; Fiscal Year: 2003
    ..Finally, new emphasis would be focused on finding the cause of chronic inflammation in the prostate with an aim towards its eradication. ..
  43. APOPTOSIS AND REPRODUCTIVE AGING OF THE FEMALE
    Jonathan Tilly; Fiscal Year: 2003
    ..It is anticipated these data will provide the first unequivocal proof that exhaustion of the follicle reserve can be delayed, if not prevented, by manipulating apoptosis-regulatory genes. ..
  44. Nutritional influences on lung function
    PATRICIA CASSANO; Fiscal Year: 2002
    ..An integrated approach to the study of nutritional factors and their potential influences on lung outcomes is proposed. ..
  45. FUNCTION OF THE STE 20 KINASE NIK IN DROSOPHILA
    Edward Skolnik; Fiscal Year: 2002
    ..NIK minus cell lines will be derived from these mice, and these cell lines will be used to determine which upstream signals are dependent upon NIK to activate the JNK MAP kinase pathway. ..
  46. SIGNALING BY THE EPS15 HOMOLOGY DOMAIN
    Michael Overduin; Fiscal Year: 2002
    ..Peptide binding interactions of unmodified and phosphorylated forms of EH domains will be structurally characterized in the presence and absence of calcium. ..
  47. Inhibitors of PAR1-G13 Interface for Arthersclerosis
    Annette Gilchrist; Fiscal Year: 2002
    ..Hundreds of billions of dollars are spent each year treating heart disease. In 1998, it was estimated that sales of cardiovascular drugs alone were $37 billion. ..
  48. Molecular Mechanisms of Receptor Function in SE
    SEVERN CHURN; Fiscal Year: 2005
    ..Understanding the cellular mechanisms that result in altered GABAAR function in SE is essential to the development of new treatment strategies to manage SE in the clinical setting. ..
  49. RECOGNITION AND SORTING OF GLYCOPROTEINS
    Michael Roth; Fiscal Year: 2002
    ....
  50. PI-3 Kinase Therapy in Mouse Tumor Models
    Ramon Parsons; Fiscal Year: 2005
    ..This information in combination with accurate measurements of tumor progression with MRI imaging will allow us to identify potential mechanisms resulting in heterogeneous responses to therapy. ..
  51. Sphingolipid modulators of vascular growth & homeostasis
    Timothy Hla; Fiscal Year: 2005
    ..These efforts will critically assess the central hypothesis that SKIEDG signaling system is a major regulator of angiogenesis. ..
  52. Mechanisms of Aryl-Hydrocarbon-Induced Ovotoxicity
    Jonathan Tilly; Fiscal Year: 2005
    ....
  53. Molecular Physiology of Voltage gated Ion Channels
    Richard Horn; Fiscal Year: 2005
    ..The goals are to determine orientations of voltage sensors and gates with respect to surrounding regions of the channel and to see how these orientations change in response to changes of membrane potential. ..
  54. Communication Between Laminin 5 and Gap Junctions
    William Carter; Fiscal Year: 2005
    ..These studies will provide an understanding of components and mechanisms critical for regulating epithelial morphogenesis in development, wound repair and tumor invasion. ..
  55. LBX2 AND UROGENITAL DEVELOPMENT
    Jonathan Epstein; Fiscal Year: 2002
    ....
  56. 2005 NNFF Consortium for NF1, NF2 and Schwannomatosis
    Jonathan Epstein; Fiscal Year: 2005
    ....
  57. PAX3 REGULATION OF CARDIAC CONOTRUNCAL DEVELOPMENT
    Jonathan Epstein; Fiscal Year: 2008
    ..Aim 3 examines the role of neural crest during development of the conduction system, and specifically examines Pax3 regulation of DCT. [unreadable] [unreadable]..
  58. Thiol-Reactive Crosslinkers for Biomaterials
    Glenn Prestwich; Fiscal Year: 2004
    ..Fourth, these new materials will be evaluated for cytocompatibility and cell growth in vitro with murine fibroblasts. Finally, selected materials will be seeded with fibroblasts and injected into nude mice for in vivo tissue generation. ..
  59. Crosslinkable Hydrogels for Tympanic Membrane Repair
    Glenn Prestwich; Fiscal Year: 2004
    ..Third, we will evaluate the biocompability and otocompability of each gel in vivo by evaluating recovery of auditory function and middle ear histology. ..
  60. Characterization of Sacl lipid phosphatases
    Peter Mayinger; Fiscal Year: 2009
    ..Insight into the regulation of lipid signals at ER and Golgi membranes will also improve our understanding of the organization of the secretory pathway. ..
  61. C-MPL AND EARLY HUMAN HEMATOPOIESIS
    William Kerr; Fiscal Year: 2003
    ..abstract_text> ..
  62. SHP-1/TYK2 INTERACTION AND ITS DEFECT IN FHLH DISEASE
    Taolin Yi; Fiscal Year: 2001
    ..These studies will make significant contributions toward achieving our long term objective of elucidating the physiological roles of SHP-1 in hematopoietic cells and defining its involvement of hematopoietic diseases. ..
  63. Mechanisms of Ataxin-1 Mediated Purkinje Cell Death
    PARMINDER VIG; Fiscal Year: 2007
    ..Further, changes observed in SCA-1 patients and in transgenic mice will be compared with that in Machado-Joseph disease/SCA-3, other cerebellar ataxias and normal controls. ..
  64. Molecular basis of vascular heterogenity and function
    Jonathan Epstein; Fiscal Year: 2008
    ..abstract_text> ..
  65. Anti-Cancer Activities of PTPase Inhibitors
    Taolin Yi; Fiscal Year: 2007
    ..abstract_text> ..
  66. SHP-1 REGULATION OF THE KU SIGNALING PATHWAY
    Taolin Yi; Fiscal Year: 2003
    ..Through these studies the investigator proposes to identify a nuclear function for SHP-1 that contributes to its role as a negative regulator of cell growth. ..
  67. SQUALENE AND OXIDOSQUALENE CYCLASES
    Glenn Prestwich; Fiscal Year: 2002
    ....
  68. PTPases as Therapeutic Targets
    Taolin Yi; Fiscal Year: 2005
    ....