endoribonucleases

Summary

Summary: A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.

Top Publications

  1. ncbi Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1
    F Urano
    Skirball Institute of Biomolecular Medicine, Departments of Medicine, Cell Biology and the Kaplan Cancer Center, New York University Medical School, New York, NY 10016, USA
    Science 287:664-6. 2000
  2. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
  3. pmc Orchestrating the unfolded protein response in health and disease
    Randal J Kaufman
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
    J Clin Invest 110:1389-98. 2002
  4. ncbi RNase E: at the interface of bacterial RNA processing and decay
    George A Mackie
    Department of Biochemistry and Molecular Biology, Life Sciences Centre, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Nat Rev Microbiol 11:45-57. 2013
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
  6. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
  7. pmc The unfolded protein response transducer IRE1α prevents ER stress-induced hepatic steatosis
    Kezhong Zhang
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48108, USA
    EMBO J 30:1357-75. 2011
  8. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
  9. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
  10. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003

Detail Information

Publications265 found, 100 shown here

  1. ncbi Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1
    F Urano
    Skirball Institute of Biomolecular Medicine, Departments of Medicine, Cell Biology and the Kaplan Cancer Center, New York University Medical School, New York, NY 10016, USA
    Science 287:664-6. 2000
    ..Dominant-negative TRAF2 inhibited activation of JNK by IRE1. Activation of JNK by endogenous signals initiated in the ER proceeds by a pathway similar to that initiated by cell surface receptors in response to extracellular signals...
  2. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
    ..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa...
  3. pmc Orchestrating the unfolded protein response in health and disease
    Randal J Kaufman
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
    J Clin Invest 110:1389-98. 2002
  4. ncbi RNase E: at the interface of bacterial RNA processing and decay
    George A Mackie
    Department of Biochemistry and Molecular Biology, Life Sciences Centre, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Nat Rev Microbiol 11:45-57. 2013
    ..I also propose a new model to rationalize the mechanism of RNase E action in the context of its localization in the bacterial cell...
  5. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  6. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  7. pmc The unfolded protein response transducer IRE1α prevents ER stress-induced hepatic steatosis
    Kezhong Zhang
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48108, USA
    EMBO J 30:1357-75. 2011
    ..The identification of IRE1α as a key regulator to prevent hepatic steatosis provides novel insights into ER stress mechanisms in fatty liver diseases associated with toxic liver injuries...
  8. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
    ..Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element...
  9. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
    ..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC...
  10. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    ..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
  11. ncbi Recognition and maturation of effector RNAs in a CRISPR interference pathway
    Emily M Gesner
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Nat Struct Mol Biol 18:688-92. 2011
    ..These structures establish the molecular basis of a specific CRISPR RNA recognition and suggest the mechanism for generation of effector RNAs responsible for gene silencing...
  12. pmc TLR activation of the transcription factor XBP1 regulates innate immune responses in macrophages
    Fabio Martinon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA
    Nat Immunol 11:411-8. 2010
    ..Our findings identify an unsuspected critical function for XBP1 in mammalian host defenses...
  13. pmc Small RNA-induced mRNA degradation achieved through both translation block and activated cleavage
    Karine Prévost
    Department of Biochemistry, RNA Group, University of Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Genes Dev 25:385-96. 2011
    ..Thus, beyond translation initiation block, sRNA-induced mRNA cleavage requires several unexpected steps, many of which are determined by structural features of the target mRNA...
  14. ncbi Dicer is essential for mouse development
    Emily Bernstein
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 35:215-7. 2003
    ..Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development...
  15. pmc Small self-RNA generated by RNase L amplifies antiviral innate immunity
    Krishnamurthy Malathi
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Nature 448:816-9. 2007
    ..Our results indicate that RNase L has an essential role in the innate antiviral immune response that relieves the requirement for direct sensing of non-self RNA...
  16. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  17. ncbi Toxins-antitoxins: diversity, evolution and function
    Finbarr Hayes
    Faculty of Life Sciences and Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
    Crit Rev Biochem Mol Biol 46:386-408. 2011
    ..Numerous toxins, many of which are sequence-specific endoribonucleases, dampen protein synthesis levels in response to a range of stress and nutritional stimuli...
  18. ncbi A time-dependent phase shift in the mammalian unfolded protein response
    Hiderou Yoshida
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8304, Japan
    Dev Cell 4:265-71. 2003
    ....
  19. ncbi Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
    ..The mode of action of eukaryotic exosome cores in RNA processing and degradation should be reconsidered, taking into account the cooperation between its multiple ribonucleolytic activities...
  20. ncbi An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3
    Dipali G Sashital
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 18:680-7. 2011
    ..These studies provide insight into a catalytically essential RNA recognition mechanism by a large class of CRISPR-related endoRNases...
  21. ncbi The unfolded protein response: integrating stress signals through the stress sensor IRE1α
    Claudio Hetz
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA
    Physiol Rev 91:1219-43. 2011
    ....
  22. ncbi Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response
    Julie Hollien
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94143, USA
    Science 313:104-7. 2006
    ..This response is well suited to complement other UPR mechanisms because it could selectively halt production of proteins that challenge the ER and clear the translocation and folding machinery for the subsequent remodeling process...
  23. pmc Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies
    Muriel Brengues
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    Science 310:486-9. 2005
    ..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
  24. pmc Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant
    Anatoly Urisman
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 2:e25. 2006
    ..These findings also raise questions about the possible relationship between exogenous infection and cancer development in genetically susceptible individuals...
  25. pmc Spatial organization of the flow of genetic information in bacteria
    Paula Montero Llopis
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Nature 466:77-81. 2010
    ..This chromosome-centric organization has important implications for cellular physiology and for our understanding of gene expression in bacteria...
  26. pmc IRE1alpha kinase activation modes control alternate endoribonuclease outputs to determine divergent cell fates
    Dan Han
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143 2520, USA
    Cell 138:562-75. 2009
    ..We propose that divergent cell fates during ER stress hinge on a balance between IRE1alpha RNase outputs that can be tilted with kinase inhibitors to favor survival...
  27. ncbi Proapoptotic BAX and BAK modulate the unfolded protein response by a direct interaction with IRE1alpha
    Claudio Hetz
    Howard Hughes Medical Institute, Dana Farber Cancer Institute, and Harvard Medical School, Boston, MA 02115, USA
    Science 312:572-6. 2006
    ..Thus, BAX and BAK function at the ER membrane to activate IRE1alpha signaling and to provide a physical link between members of the core apoptotic pathway and the UPR...
  28. pmc Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs
    A van Hoof
    Department of Molecular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Mol Cell Biol 20:441-52. 2000
    ..This finding suggests that the cytoplasmic and nuclear forms of the exosome represent two functionally different complexes involved in distinct 3'-to-5' processing and degradation reactions...
  29. pmc Ribonucleases J1 and J2: two novel endoribonucleases in B.subtilis with functional homology to E.coli RNase E
    Sergine Even
    CNRS UPR9073, IBPC 13 rue P et M Curie, 75005 Paris, France
    Nucleic Acids Res 33:2141-52. 2005
    ..we report the purification and identification by mass spectrometry in Bacillus subtilis of two paralogous endoribonucleases, here named RNases J1 and J2, which share functional homologies with RNase E but no sequence similarity...
  30. pmc Processing bodies require RNA for assembly and contain nontranslating mRNAs
    Daniela Teixeira
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
    RNA 11:371-82. 2005
    ..Fourth, multiple translation factors and ribosomal proteins are lacking from P-bodies. These results suggest additional biological roles of P-bodies in addition to being sites of mRNA degradation...
  31. ncbi Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping
    Martin Fenger-Grøn
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 80309, USA
    Mol Cell 20:905-15. 2005
    ..These observations suggest that multiple proteins involved in human decapping are important subunits of PBs and are activated on ARE-mRNAs by the protein TTP...
  32. pmc Binding and cleavage of CRISPR RNA by Cas6
    Jason Carte
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    RNA 16:2181-8. 2010
    ..Finally, we show that P. furiosus Cas6 remains stably associated with its cleavage products, suggesting additional roles for Cas6 in psiRNA biogenesis...
  33. ncbi Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation
    Verena Pfeiffer
    Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
    Nat Struct Mol Biol 16:840-6. 2009
    ..The discovery of CDS targeting markedly expands the sequence space for sRNA target predictions in bacteria...
  34. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
    ..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
  35. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  36. pmc New insights into the role of RNase L in innate immunity
    Arindam Chakrabarti
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Interferon Cytokine Res 31:49-57. 2011
    ..These newly described findings about RNase L could eventually provide the basis for developing broad-spectrum antimicrobial drugs...
  37. pmc Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
    Genes Dev 19:2355-66. 2005
    ..MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear...
  38. ncbi MicroRNA expression is required for pancreatic islet cell genesis in the mouse
    Francis C Lynn
    Diabetes Center, Hormone Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143 0534, USA
    Diabetes 56:2938-45. 2007
    ..We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development...
  39. ncbi Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome
    Junjun Gao
    Institute for Cell and Molecular Biology, University of Texas, TX 78712, USA
    Mol Microbiol 61:394-406. 2006
    ..coli...
  40. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
    ..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41)...
  41. pmc A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats
    Natalia Beloglazova
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    J Biol Chem 283:20361-71. 2008
    ..Here we show that the CAS2 proteins represent a novel family of endoribonucleases. Six purified CAS2 proteins from diverse organisms cleaved single-stranded RNAs preferentially within U-rich ..
  42. pmc Fine-tuning of the unfolded protein response: Assembling the IRE1alpha interactome
    Claudio Hetz
    The FONDAP Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago, Chile
    Mol Cell 35:551-61. 2009
    ..In this Review, we discuss recent evidence supporting a model where IRE1alpha signaling emerges as a highly regulated process, controlled by the formation of a dynamic scaffold onto which many regulatory components assemble...
  43. ncbi Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast
    Feng He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Cell 12:1439-52. 2003
    ....
  44. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  45. pmc Interferon action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase L
    A Zhou
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
    EMBO J 16:6355-63. 1997
    ..Another implication is that the 2-5A system is likely to contribute to the antiviral activity of interferon by inducing apoptosis of infected cells...
  46. pmc Mammalian endoplasmic reticulum stress sensor IRE1 signals by dynamic clustering
    Han Li
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:16113-8. 2010
    ..Thus IRE1 activity is governed by a timer that may be important in switching the UPR from the initially cytoprotective phase to the apoptotic mode...
  47. pmc Ribonuclease activity and RNA binding of recombinant human Dicer
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
    EMBO J 21:5864-74. 2002
    ..Availability of the recombinant Dicer protein will help improve our understanding of RNA silencing and other Dicer-related processes...
  48. ncbi Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals
    C Patil
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    Curr Opin Cell Biol 13:349-55. 2001
    ..Recent results reveal that the output of the mammalian UPR reaches beyond transcriptional regulation of secretory pathway components to control of general translation, the cell cycle and programmed cell death...
  49. pmc Divergent effects of PERK and IRE1 signaling on cell viability
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e4170. 2009
    ..These results demonstrate that extended PERK and IRE1 signaling have opposite effects on cell viability. Differential activation of PERK and IRE1 may determine life or death decisions after ER protein misfolding...
  50. pmc PKR and RNase L contribute to protection against lethal West Nile Virus infection by controlling early viral spread in the periphery and replication in neurons
    Melanie A Samuel
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:7009-19. 2006
    ..Our data suggest that PKR and RNase L contribute to IFN-mediated protection in a cell-restricted manner and control WNV infection in peripheral tissues and some neuronal subtypes...
  51. ncbi Mutations in the RNA component of RNase MRP cause a pleiotropic human disease, cartilage-hair hypoplasia
    M Ridanpaa
    Folkhalsan Institute of Genetics, 00280 Helsinki, Finland
    Cell 104:195-203. 2001
    ..The association of protein subunits with RNA appears unaltered. We conclude that mutations in RMRP cause CHH by disrupting a function of RNase MRP RNA that affects multiple organ systems...
  52. pmc Role of polynucleotide phosphorylase in sRNA function in Escherichia coli
    Nicholas De Lay
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    RNA 17:1172-89. 2011
    ....
  53. pmc 3' end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNA
    Jeremy E Wilusz
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell 135:919-32. 2008
    ..Our findings reveal a 3' end processing mechanism by which a single gene locus can yield both a stable nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasmic RNA...
  54. ncbi Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coli
    Yoshiki Ikeda
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 79:419-32. 2011
    ..Finally, we demonstrate that overexpression of RhlB partially inhibits the Hfq binding to RNase E and the rapid degradation of ptsG mRNA...
  55. pmc Human cytomegalovirus infection activates and regulates the unfolded protein response
    Jennifer A Isler
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, USA
    J Virol 79:6890-9. 2005
    ..These results suggest that, although HCMV infection induces the unfolded protein response, it modifies the outcome to benefit viral replication...
  56. pmc Of proteins and RNA: the RNase P/MRP family
    Olga Esakova
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    RNA 16:1725-47. 2010
    ..We review current information on RNase P and the closely related universal eukaryotic enzyme RNase MRP, focusing on their functions and structural organization...
  57. ncbi Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
  58. ncbi Endonucleolytic initiation of mRNA decay in Escherichia coli
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS et Université Paul Sabatier, 31062 Toulouse, France
    Prog Mol Biol Transl Sci 85:91-135. 2009
    ..In E. coli, the turnover of mRNA involves multiple, redundant pathways involving 3'-exoribonucleases, endoribonucleases, and a variety of other enzymes that modify RNA covalently or affect its conformation...
  59. pmc The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response
    Jiahai Zhou
    Life Sciences Institute, LSI 3163B, Department of Biological Chemistry, University of Michigan Medical Center, 210 Washtenaw Avenue, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 103:14343-8. 2006
    ..Our structural, biochemical, and functional studies in vivo altogether demonstrate that IRE1 and PERK have conserved a common molecular interface necessary and sufficient for dimerization and UPR signaling...
  60. pmc Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins"
    Shahar Amitai
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 5:e1000390. 2009
    ....
  61. pmc A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase L
    Jian Qiu Han
    Department of Microbiology, School of Medicine, University of Colorado, Mail Stop 8333, Room P18 9116, 12800 E 19th Ave, Aurora, CO 80045, USA
    J Virol 81:5561-72. 2007
    ....
  62. pmc Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli
    Yi Chun Tsai
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Nucleic Acids Res 40:10417-31. 2012
    ....
  63. pmc Antagonism of the interferon-induced OAS-RNase L pathway by murine coronavirus ns2 protein is required for virus replication and liver pathology
    Ling Zhao
    Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell Host Microbe 11:607-16. 2012
    ..Thus, RNase L is a critical cellular factor for protection against viral infection of the liver and the resulting hepatitis...
  64. ncbi Two pathways for RNase E action in Escherichia coli in vivo and bypass of its essentiality in mutants defective for Rho-dependent transcription termination
    K Anupama
    Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 001, India
    Mol Microbiol 82:1330-48. 2011
    ....
  65. pmc Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase
    Jung Ho Park
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    FEBS Lett 585:2526-32. 2011
    ..This cleavage site is over-represented in the B. subtilis genes associated with biosynthesis of secondary metabolites, suggesting that MazF-bs may be involved in the regulation of the production of secondary metabolites...
  66. pmc Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA
    Yvonne Göpel
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
    Genes Dev 27:552-64. 2013
    ..Thus, in analogy to regulated proteolysis, RapZ is an adaptor, and GlmY is an anti-adaptor in regulated turnover of a regulatory small RNA...
  67. pmc Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease
    Sandy Mattijssen
    Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    Cell Mol Life Sci 68:2469-80. 2011
    ..This is the first study providing direct evidence for the cleavage of a mRNA by RNase MRP/RNase P in human cells. Implications for the involvement in the pathophysiology of CHH are discussed...
  68. pmc The social fabric of the RNA degradosome
    Katarzyna J Bandyra
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Biochim Biophys Acta 1829:514-22. 2013
    ..This article is part of a Special Issue entitled: RNA Decay mechanisms...
  69. pmc Role of RNase MRP in viral RNA degradation and RNA recombination
    Hannah M Jaag
    Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546 0091, USA
    J Virol 85:243-53. 2011
    ..To identify host endoribonucleases involved in degradation of Tomato bushy stunt virus (TBSV) in a Saccharomyces cerevisiae model host, we ..
  70. pmc RNase L releases a small RNA from HCV RNA that refolds into a potent PAMP
    Krishnamurthy Malathi
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    RNA 16:2108-19. 2010
    ..It is proposed that RNase L mediated cleavage of HCV RNA generates svRNA that activates RIG-I, thus propagating innate immune signaling to the IFN-β gene...
  71. pmc RNase L plays a role in the antiviral response to West Nile virus
    Svetlana V Scherbik
    Department of Biology, Georgia State University, P O Box 4010, Atlanta, Georgia 30302 4010, USA
    J Virol 80:2987-99. 2006
    ..The results suggest that RNase L activation is not a major component of the Oas1b-mediated flavivirus resistance phenotype...
  72. pmc RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis
    Karen Shahbabian
    Institut de Biologie Physico Chimique, CNRS UPR9073, affiliated with Université de Paris 7 Denis Diderot, Paris, France
    EMBO J 28:3523-33. 2009
    ..This indicates that RNase Y might be not only important for riboswitch RNA turnover but also as a key player in the initiation of mRNA decay in B. subtilis. About 40% of the sequenced eubacterial species have an RNase Y orthologue...
  73. pmc Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB
    Taras Afonyushkin
    Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter Dr Bohrgasse 9 4, A 1030 Vienna, Austria
    Nucleic Acids Res 33:1678-89. 2005
    ..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover...
  74. ncbi Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineage
    Sergei B Koralov
    Immune Disease Institute and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 132:860-74. 2008
    ....
  75. pmc Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex
    Christelle M Roux
    Department of Microbiology and Immunology, University of Rochester Medical Center, School of Medicine and Dentistry, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Bacteriol 193:5520-6. 2011
    ..Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms...
  76. ncbi Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'
    Annie Prud'homme-Généreux
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 54:1409-21. 2004
    ..These findings show that the RNA degradosome is a flexible macromolecular machine capable of adapting to altered environmental conditions...
  77. ncbi Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover
    Akio Yamashita
    Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, Texas 77030, USA
    Nat Struct Mol Biol 12:1054-63. 2005
    ..Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay...
  78. pmc Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae
    Je Hyun Yoon
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 189:813-27. 2010
    ..These results demonstrate that Ste20 has an unexpected role in the modulation of mRNA decay and translation and that phosphorylation of Dcp2 is an important control point for mRNA decapping...
  79. pmc Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs
    Isabella Moll
    Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    RNA 9:1308-14. 2003
    ..It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack...
  80. pmc Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
    Astrid D Haase
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Genes Dev 24:2499-504. 2010
    ..Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis...
  81. pmc Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E
    Kazushi Suzuki
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Genes Dev 20:2605-17. 2006
    ..Thus, these studies demonstrate a novel process for the selective targeting of RNA molecules for degradation by RNase E and a novel function for a GGDEF-EAL protein...
  82. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  83. ncbi The making of tRNAs and more - RNase P and tRNase Z
    Roland K Hartmann
    Philipps Universitat Marburg, Institut fur Pharmazeutische Chemie, Marbacher Weg 6, D 35037 Marburg, Germany
    Prog Mol Biol Transl Sci 85:319-68. 2009
    ..The current knowledge on prokaryotic tRNase Z in tRNA 3'-processing is reviewed here. A second, tRNase Z-independent pathway of tRNA 3'-end maturation involving 3'-exonucleases will also be discussed...
  84. pmc A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral MicroRNAs
    Hal P Bogerd
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell 37:135-42. 2010
    ....
  85. ncbi Bypassing a kinase activity with an ATP-competitive drug
    Feroz R Papa
    Department of Medicine, University of California, San Francisco, CA 94143 2200, USA
    Science 302:1533-7. 2003
    ..Thus, rather than through phosphorylation per se, a conformational change in the kinase domain triggered by occupancy of the active site with a ligand leads to activation of all known downstream functions...
  86. pmc Identification of a consensus element recognized and cleaved by IRE1 alpha
    Daisuke Oikawa
    Iwawaki Initiative Research Unit, Advanced Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nucleic Acids Res 38:6265-73. 2010
    ..The sequence and structure were also conserved in the known IRE1α cleavage targets, CD59 and XBP1. These findings provide the important clue to understanding the molecular mechanisms by which IRE1α recognizes and cleaves target RNAs...
  87. ncbi The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBK
    Maria Belitsky
    Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel Canada, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Mol Cell 41:625-35. 2011
    ..These findings are intriguing, since most known quorum-sensing molecules monitor gene expression on the transcriptional level, while EDF monitors posttranscriptionally...
  88. pmc RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli
    Martin Lehnik-Habrink
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    J Bacteriol 193:5431-41. 2011
    ..The results presented in this study provide novel evidence for the idea that RNase Y is the functional equivalent of RNase E, even though the two enzymes do not share any sequence similarity...
  89. pmc ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats
    Hideki Nishitoh
    Laboratory of Cell Signaling, Graduate School, Tokyo Medical and Dental University, Bunkyo ku 113 8549, Japan
    Genes Dev 16:1345-55. 2002
    ..These findings suggest that ASK1 is a key element in ER stress-induced cell death that plays an important role in the neuropathological alterations in polyQ diseases...
  90. pmc An RNA degradosome assembly in Caulobacter crescentus
    Steven W Hardwick
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Nucleic Acids Res 39:1449-59. 2011
    ..Finally, we observe that the abundance of RNase E varies through the cell cycle, with maxima at morphological differentiation and cell division. This variation may contribute to the program of gene expression during cell division...
  91. pmc The RNA of RNase MRP is required for normal processing of ribosomal RNA
    S Chu
    Department of Biology, University of Rochester, NY 14627
    Proc Natl Acad Sci U S A 91:659-63. 1994
    ..A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing...
  92. pmc RNAi-mediated targeting of heterochromatin by the RITS complex
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
    Science 303:672-6. 2004
    ..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
  93. ncbi mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems
    Yoshihiro Yamaguchi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Prog Mol Biol Transl Sci 85:467-500. 2009
    ..We propose that mRNA interferases play roles not only in cell growth regulation and programmed cell death, but also in regulation of specific gene expression (either positively or negatively) in bacteria...
  94. pmc Influenza A viral replication is blocked by inhibition of the inositol-requiring enzyme 1 (IRE1) stress pathway
    Ihab H Hassan
    Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 287:4679-89. 2012
    ..Decreasing viral replication by modulating the host ER stress response is a novel strategy that has important therapeutic implications...
  95. ncbi From conformational chaos to robust regulation: the structure and function of the multi-enzyme RNA degradosome
    Maria W Górna
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Q Rev Biophys 45:105-45. 2012
    ..Parallels are drawn with analogous machinery in organisms from all life domains. Finally, we conjecture on roles of the degradosome as a regulatory hub for complex cellular processes...
  96. ncbi On the role of RNA amplification in dsRNA-triggered gene silencing
    T Sijen
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Cell 107:465-76. 2001
    ..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
  97. pmc Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coli
    Sergio Martinez Rodriguez
    Laboratorium voor Ultrastructuur, Vrije Universiteit Brussels, Pleinlaan 2, B 1050 Brussels, Belgium
    Protein Sci 17:681-90. 2008
    ..This B1 base recognition site is highly variable among bacterial sequences and the observed interactions are unique to EcRNaseI and a few close relatives...
  98. ncbi Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor
    A Oguro
    Institute of Biological Sciences, University of Tsukuba, Tsukuba shi, Ibaraki 305, Japan
    J Biol Chem 273:19542-7. 1998
    ..It is concluded that Bs-RNase III is an enzyme that processes pre-scRNA...
  99. pmc RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming ability
    Masaru Tamura
    Stanford University School of Medicine, Department of Genetics, 300 Pasteur Drive, Stanford, CA 94305 5120, USA
    J Bacteriol 188:5145-52. 2006
    ..Our results demonstrate that RNase E activity is required to maintain a proper cellular ratio of the FtsZ and FtsA proteins in E. coli but that FtsZ deficiency does not account for the nonviability of cells lacking RNase E...
  100. pmc HDLs inhibit endoplasmic reticulum stress and autophagic response induced by oxidized LDLs
    C Muller
    Inserm U 858, Department of Vascular Biology, IFR 150, CHU Rangueil, Toulouse, France
    Cell Death Differ 18:817-28. 2011
    ..Finally, the cytosolic Ca(2+) deregulation triggered by oxLDLs is a common signaling pathway that mediates ER stress-induced cell death and autophagy, all these events being blocked by HDLs...
  101. pmc Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiation
    Walter Rossmanith
    Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria
    PLoS ONE 6:e19152. 2011
    ..Our data suggest that RNase Z(L) is the enzyme involved in both, nuclear and mitochondrial tRNA 3' end maturation...

Research Grants62

  1. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
    ..dsRNA cleavage is catalyzed by members of the ribonuclease III (RNase III) family of endoribonucleases, which are highly conserved in bacterial and eukaryotic cells...
  2. Mechanisms of ribosomal reactions: peptide bond formation, peptide release and mR
    Scott A Strobel; Fiscal Year: 2013
    ..Although RelE is structurally similar to the general family of endoribonucleases, it does not contain any of the residues expected to be important for catalysis...
  3. Ribonuclease III mechanism of double-strand RNA cleavage
    ALLEN NICHOLSON; Fiscal Year: 2003
    ..Double-stranded (ds) RNA cleavage is carried out by members of the ribonuclease Ill superfamily of endoribonucleases. RNase III orthologues participate in mRNA and rRNA maturation, mRNA degradation, and antisense RNA action...
  4. Role of Phospholipid Scramblase in Interferon Action
    Robert Silverman; Fiscal Year: 2005
    ..abstract_text> ..
  5. Role of RNase L in the Biology of Prostate Cancer
    Robert Silverman; Fiscal Year: 2008
    ..The experiments described in this PROPOSAL describe a previously unexplored area of research that may bring us closer to the eventual goal of understanding a molecular basis for prostate cancer. ..
  6. High Throughput Chemical Genomics to Identify Novel Inhibitors of CHOP
    Randal Kaufman; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  7. The Role of CXCL9 in Genital HSV-2 Infection
    Daniel Carr; Fiscal Year: 2009
    ..g., vaccine or gene transfer) to reduce the incidence of infection or reduce the capacity of latent virus to reactivate. ..
  8. Unfolded proteins in the Endoplasmic Reticulum to Disease
    Randal Kaufman; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  9. Unfolded Protein Response in Glucose Homeostasis
    Randal Kaufman; Fiscal Year: 2006
    ..These in vivo models have the potential for providing novel biologic insights into human disease and may also facilitate testing of new therapies. [unreadable] [unreadable] [unreadable]..
  10. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
  11. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    David H Bechhofer; Fiscal Year: 2010
    ..The likely participation of 2 B. subtilis endoribonucleases -- RNase J1 and RNase J2 -in mRNA decay will be assessed...
  12. Beta-globin mRNA decay in erythroid cells
    Daniel Schoenberg; Fiscal Year: 2005
    ..The long-term goal is to identify new therapeutic targets for treating beta-thalassemia and other diseases caused by PTCs by better understanding the enzymatic mechanisms responsible for the degradation of PTC-containing mRNAs. ..
  13. Anti-Viral Gene Delivery in the Nervous System
    Daniel Carr; Fiscal Year: 2005
    ..abstract_text> ..
  14. REGULATED POLYADENYLATION OF MESSENGER RNA
    Daniel Schoenberg; Fiscal Year: 2004
    ....
  15. COORDINATION OF BACTERIAL RIBOSOMAL PROTEIN SYNTHESIS
    Lasse Lindahl; Fiscal Year: 2001
    ....
  16. TRANSLATIONAL CONTROL IN REGULATION OF APOPTOSIS
    Randal Kaufman; Fiscal Year: 2002
    ....
  17. NEUROIMMUNOLOGY OF AED IN OCULAR HSV-1 INFECTION
    Daniel Carr; Fiscal Year: 2001
    ..abstract_text> ..
  18. Conformational Diseases of the Secretory Pathway
    Randal Kaufman; Fiscal Year: 2003
    ..This conference will focus on new technologies and methodologies to study this rapidly moving field in order to dissect the complexity and diversity of ER function. ..
  19. Increasing 2-5A effectiveness through better chemistry
    Hagen Cramer; Fiscal Year: 2003
    ..Ridgeway Biosystems Inc. will apply successful chemical modifications to other therapeutics currently under development. ..
  20. A FADD-Associated Helicase Essential for Host Defense
    Glen Barber; Fiscal Year: 2008
    ..We anticipate that these studies will improve our knowledge of pathogenesis and provide new concepts for the rational design of novel therapeutic and vaccine strategies. [unreadable] [unreadable] [unreadable]..
  21. Role of the Unfolded Protein Response in Type 2 Diabetes
    FEROZ PAPA; Fiscal Year: 2008
    ..The UCSF Department of Medicine is fully committed to Dr. Papa's career development, and will make all necessary resources available to facilitate the success of the project. ..
  22. Role of PKR in Growth Regulation
    Glen Barber; Fiscal Year: 2008
    ..We propose that these aims will lend insight into mechanisms of cell signaling, tumorigenesis and interferon-mediated actions in the cell. ..
  23. Molecular identification of a sialyl 9-O-acetyl transferase
    Shiv Pillai; Fiscal Year: 2008
    ..The gene that codes for an enzyme that controls the strength of the immune response will be studied. This gene may be an important target for the treatment of lupus and other related diseases. [unreadable] [unreadable] [unreadable]..
  24. Targeting RNA to develop novel antibacterial agents
    CHRISTOPHER TROTTA; Fiscal Year: 2003
    ..Broad-spectrum inhibitors will be further tested for inhibition of the eucaryal RNase P enzyme and cytotoxicity against a panel of mammalian cell lines. ..
  25. Estrogen regulation of uterine microRNAs: Novel factors in MMP-9 regulation
    Warren Nothnick; Fiscal Year: 2008
    ..Enhancing our understanding on the regulation of MMP-9 expression may lead to the establishment of novel approaches to counteract mis-expression of this protease and the diseases associated with it. [unreadable] [unreadable] [unreadable]..
  26. MECHANISM OF INTERFERON ACTION
    CHARLES SAMUEL; Fiscal Year: 2009
    ..Furthermore, elucidation of the actions of IFN at the molecular level is of importance in view of potential applications of interferon in the clinic. ..
  27. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2009
    ..abstract_text> ..
  28. Catalytic Mechanisms of RNA Ezymes
    Martha J Fedor; Fiscal Year: 2010
    ..The results of these studies also will provide a framework to guide the development of technical and therapeutic applications involving RNAs as targets and reagents. ..
  29. Mechanisms of VSV-mediated Oncolysis
    Glen N Barber; Fiscal Year: 2010
    ..Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
  30. Impact of the interferon regulated proteins XAF1 and ZNF313 on innate immunity
    Douglas W Leaman; Fiscal Year: 2010
    ....
  31. Targeted Therapy for Burkitt Lymphoma in Resource Poor Settings
    Glen N Barber; Fiscal Year: 2010
    ..Our long term goal is to develop a targeted, efficacious, antiviral-based therapy for EBV (HIV) associated lymphoma that is applicable in developing nations. ..
  32. 2007 Epigenetics Gordon Research Conference
    Steven Jacobsen; Fiscal Year: 2007
    ..Perhaps equally importantly, it will launch the careers of new scientists in this frontier area. [unreadable] [unreadable] [unreadable]..
  33. Innate Intracellular Mechanisms
    Glen N Barber; Fiscal Year: 2010
    ..The importance of STING in innate signaling will be determined. Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
  34. Role of the Yeast Lsm Proteins in mRNA Decay
    THARUN SUNDARESAN; Fiscal Year: 2010
    ..abstract_text> ..
  35. mTOR Signaling In Chronic Myelogenous Leukemia
    Hung Fan; Fiscal Year: 2009
    ..abstract_text> ..
  36. MEMBRANE IMMUNOGLOBULIN ASSEMBLY AND FUNCTION
    Shiv Pillai; Fiscal Year: 2002
    ..Since the majority of human lymphomas develop form activated B-cells, these signaling mechanisms are likely to be of direct relevance for a significant proportion of human cancers. ..
  37. The role of PKK in NFkappa B activation
    Shiv Pillai; Fiscal Year: 2007
    ..PKK represents a novel enzyme that may be critical in pathways that are important in cancer, inflammation, cell survival, and differentiation. ..
  38. Nitrosylcobalamin - A Novel Chemotherapeutic Agent
    Daniel Lindner; Fiscal Year: 2007
    ..Determine whether inhibition of TCII-R expression causes resistance to NO-Cbl d. Define effects of IFN-beta upon S-nitrosylation of TRAIL receptors and PRL phosphatase ..
  39. Molecular Mediators of Interferon induced Apoptosis
    Douglas Leaman; Fiscal Year: 2005
    ..The results of these studies should provide important information on the molecular details of antitumor activity of IFNs that can be used to identify or develop targeted therapeutics that augment IFN effects. ..
  40. 2-5A Antisense for Degradation of SARS Coronavirus RNA
    Hagen Cramer; Fiscal Year: 2004
    ..We propose to develop an effective and marketable 2-5A antisense drug for the treatment of SARS infection. ..
  41. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2004
    ....
  42. Viral DNA Replication and Late Transcription
    Linda Guarino; Fiscal Year: 2004
    ..Knowledge gained from these experiments should be relevant to other viral systems and will provide a better understanding of the complex interactions between transcription and replication machinery in eukaryotic cells. ..
  43. Cold Shock Vectors for Single Protein Production
    Masayori Inouye; Fiscal Year: 2004
    ..The proposed vector-host system will be highly useful not only for structural biology but also for medicinal research involving human proteins, which can not be obtained by conventional expression systems. ..
  44. Screening for Rnase L Activators
    Hagen Cramer; Fiscal Year: 2004
    ..The successful completion of this proposed project will open a whole new class of therapy for currently intractable cancer and viral infections leveraging an important pathway of the body's natural immunity. ..
  45. Marginal zone B lymphocytes and blood borne pathogens
    Shiv Pillai; Fiscal Year: 2004
    ..Finally we will attempt to determine whether antigen-specific MZ B cells, as opposed to follicuar B cells, can readily capture and present blood borne antigens, and thus activate na'fve T cells. ..
  46. FUNCTION ANALYSIS OF THE DSRNA REGULATED NUCLEAR FACTORS
    Glen Barber; Fiscal Year: 2004
    ..abstract_text> ..
  47. Studies on human peripheral B lymphocytes
    Shiv Pillai; Fiscal Year: 2005
    ....
  48. EXPRESSION AND PATHOGENESIS OF MURINE LEUKEMIA VIRUS
    Hung Fan; Fiscal Year: 2005
    ..low expression. Together, these experiments will provide new and important insights into leukemogenesis by MuLVs. ..
  49. Tat-Driven Delivery of 2-5A Cargo for HIV RNA Ablation
    PAUL TORRENCE; Fiscal Year: 2003
    ..This Tat lector-driven transport of 2-5A cargo would provide a major advantage over standard antisense nucleiccid strategies that suffer from inefficient cellular uptake, ..
  50. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2003
    ..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
  51. Inositol hexakisphosphate kinase 2:apoptosis regulator
    Daniel Lindner; Fiscal Year: 2006
    ..Determine the relevance of IP6K2 domains for IFN- beta-therapy in vivo - Assess IP6K2 mutants grownas xenografts. ..
  52. The Role of TIMP-1 in Uterine Physiology
    Warren Nothnick; Fiscal Year: 2005
    ..These studies will broaden our understanding on the role of TIMP-1 in uterine physiology and may help to develop new strategies to treat uterine disorders such as infertility, endometriosis and uterine cancer. ..
  53. MOLECULAR BIOLOGY OF JAAGSIEKTE SHEEP RETROVIRUS
    Hung Fan; Fiscal Year: 2003
    ..These experiments will provide insight into JSRV-induced lung cancer in sheep, and possibly into human BAC. ..
  54. Nucleic Acids Gordon Research Conference 2005
    MARTHA FEDOR; Fiscal Year: 2005
    ..Considerable time is allotted for discussion following formal lectures. Poster sessions and optional recreational activities will stimulate informal interactions. ..
  55. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 2005
    ..abstract_text> ..
  56. Novel Targets for Treatment of Smallpox
    Stewart Shuman; Fiscal Year: 2003
    ....
  57. The Mammalian Signal Recognition Particle
    KEVIN WEEKS; Fiscal Year: 2006
    ..Moreover, the work is designed to identify principles generalizable to other biologically and medically prominent ribonucleoprotein complexes. ..
  58. Protein-Facilitates RNA Catalysts
    KEVIN WEEKS; Fiscal Year: 2007
    ..5) To begin to explore the molecular mechanism by which Mrs1 facilitates catalysis of the bl3 group I intron. (6) To develop a high resolution model for the maturase-bl3 RNA complex. ..
  59. Proteomics of Cerebrospinal Fluid in Chronic Fatigue Syndrome
    James Baraniuk; Fiscal Year: 2009
    ..These methods and biomarkers may be of diagnostic value. They will be useful for assessing longitudinal changes in disease severity, phenotype, or the effects of treatment. ..
  60. Age and Sleep Effects on ER Protein Function
    Nirinjini Naidoo; Fiscal Year: 2005
    ..We will use 2-D DIGE, as well as oxidation assays and mass spectrometry, to identify proteins that are altered by aging and sleep deprivation in the ER. ..