Genomes and Genes
Summary: A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.
Publications265 found, 100 shown here
- Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1F Urano
Skirball Institute of Biomolecular Medicine, Departments of Medicine, Cell Biology and the Kaplan Cancer Center, New York University Medical School, New York, NY 10016, USA
Science 287:664-6. 2000..Dominant-negative TRAF2 inhibited activation of JNK by IRE1. Activation of JNK by endogenous signals initiated in the ER proceeds by a pathway similar to that initiated by cell surface receptors in response to extracellular signals...
- IRE1 signaling affects cell fate during the unfolded protein responseJonathan H Lin
Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
Science 318:944-9. 2007..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa...
- Orchestrating the unfolded protein response in health and diseaseRandal J Kaufman
Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
J Clin Invest 110:1389-98. 2002
- RNase E: at the interface of bacterial RNA processing and decayGeorge A Mackie
Department of Biochemistry and Molecular Biology, Life Sciences Centre, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
Nat Rev Microbiol 11:45-57. 2013..I also propose a new model to rationalize the mechanism of RNase E action in the context of its localization in the bacterial cell...
- The nuclear RNase III Drosha initiates microRNA processingYoontae Lee
Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
Nature 425:415-9. 2003..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
- Decapping and decay of messenger RNA occur in cytoplasmic processing bodiesUjwal Sheth
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
Science 300:805-8. 2003..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
- The unfolded protein response transducer IRE1α prevents ER stress-induced hepatic steatosisKezhong Zhang
Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48108, USA
EMBO J 30:1357-75. 2011..The identification of IRE1α as a key regulator to prevent hepatic steatosis provides novel insights into ER stress mechanisms in fatty liver diseases associated with toxic liver injuries...
- The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosomeClaudia Schneider
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
Nucleic Acids Res 37:1127-40. 2009..Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element...
- R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathwayQinghua Liu
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
Science 301:1921-5. 2003..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC...
- Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coliEric Massé
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genes Dev 17:2374-83. 2003..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
- Recognition and maturation of effector RNAs in a CRISPR interference pathwayEmily M Gesner
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
Nat Struct Mol Biol 18:688-92. 2011..These structures establish the molecular basis of a specific CRISPR RNA recognition and suggest the mechanism for generation of effector RNAs responsible for gene silencing...
- TLR activation of the transcription factor XBP1 regulates innate immune responses in macrophagesFabio Martinon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA
Nat Immunol 11:411-8. 2010..Our findings identify an unsuspected critical function for XBP1 in mammalian host defenses...
- Small RNA-induced mRNA degradation achieved through both translation block and activated cleavageKarine Prévost
Department of Biochemistry, RNA Group, University of Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
Genes Dev 25:385-96. 2011..Thus, beyond translation initiation block, sRNA-induced mRNA cleavage requires several unexpected steps, many of which are determined by structural features of the target mRNA...
- Dicer is essential for mouse developmentEmily Bernstein
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nat Genet 35:215-7. 2003..Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development...
- Small self-RNA generated by RNase L amplifies antiviral innate immunityKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
Nature 448:816-9. 2007..Our results indicate that RNase L has an essential role in the innate antiviral immune response that relieves the requirement for direct sensing of non-self RNA...
- The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase EAgamemnon J Carpousis
Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
Annu Rev Microbiol 61:71-87. 2007....
- Toxins-antitoxins: diversity, evolution and functionFinbarr Hayes
Faculty of Life Sciences and Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
Crit Rev Biochem Mol Biol 46:386-408. 2011..Numerous toxins, many of which are sequence-specific endoribonucleases, dampen protein synthesis levels in response to a range of stress and nutritional stimuli...
- A time-dependent phase shift in the mammalian unfolded protein responseHiderou Yoshida
Graduate School of Biostudies, Kyoto University, Kyoto 606 8304, Japan
Dev Cell 4:265-71. 2003....
- Endonucleolytic RNA cleavage by a eukaryotic exosomeAlice Lebreton
Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
Nature 456:993-6. 2008..The mode of action of eukaryotic exosome cores in RNA processing and degradation should be reconsidered, taking into account the cooperation between its multiple ribonucleolytic activities...
- An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3Dipali G Sashital
Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
Nat Struct Mol Biol 18:680-7. 2011..These studies provide insight into a catalytically essential RNA recognition mechanism by a large class of CRISPR-related endoRNases...
- The unfolded protein response: integrating stress signals through the stress sensor IRE1αClaudio Hetz
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA
Physiol Rev 91:1219-43. 2011....
- Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein responseJulie Hollien
Department of Cellular and Molecular Pharmacology, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94143, USA
Science 313:104-7. 2006..This response is well suited to complement other UPR mechanisms because it could selectively halt production of proteins that challenge the ER and clear the translocation and folding machinery for the subsequent remodeling process...
- Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodiesMuriel Brengues
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
Science 310:486-9. 2005..Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage...
- Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variantAnatoly Urisman
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
PLoS Pathog 2:e25. 2006..These findings also raise questions about the possible relationship between exogenous infection and cancer development in genetically susceptible individuals...
- Spatial organization of the flow of genetic information in bacteriaPaula Montero Llopis
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
Nature 466:77-81. 2010..This chromosome-centric organization has important implications for cellular physiology and for our understanding of gene expression in bacteria...
- IRE1alpha kinase activation modes control alternate endoribonuclease outputs to determine divergent cell fatesDan Han
Department of Medicine, University of California, San Francisco, San Francisco, CA 94143 2520, USA
Cell 138:562-75. 2009..We propose that divergent cell fates during ER stress hinge on a balance between IRE1alpha RNase outputs that can be tilted with kinase inhibitors to favor survival...
- Proapoptotic BAX and BAK modulate the unfolded protein response by a direct interaction with IRE1alphaClaudio Hetz
Howard Hughes Medical Institute, Dana Farber Cancer Institute, and Harvard Medical School, Boston, MA 02115, USA
Science 312:572-6. 2006..Thus, BAX and BAK function at the ER membrane to activate IRE1alpha signaling and to provide a physical link between members of the core apoptotic pathway and the UPR...
- Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAsA van Hoof
Department of Molecular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
Mol Cell Biol 20:441-52. 2000..This finding suggests that the cytoplasmic and nuclear forms of the exosome represent two functionally different complexes involved in distinct 3'-to-5' processing and degradation reactions...
- Ribonucleases J1 and J2: two novel endoribonucleases in B.subtilis with functional homology to E.coli RNase ESergine Even
CNRS UPR9073, IBPC 13 rue P et M Curie, 75005 Paris, France
Nucleic Acids Res 33:2141-52. 2005..we report the purification and identification by mass spectrometry in Bacillus subtilis of two paralogous endoribonucleases, here named RNases J1 and J2, which share functional homologies with RNase E but no sequence similarity...
- Processing bodies require RNA for assembly and contain nontranslating mRNAsDaniela Teixeira
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
RNA 11:371-82. 2005..Fourth, multiple translation factors and ribosomal proteins are lacking from P-bodies. These results suggest additional biological roles of P-bodies in addition to being sites of mRNA degradation...
- Multiple processing body factors and the ARE binding protein TTP activate mRNA decappingMartin Fenger-Grøn
Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 80309, USA
Mol Cell 20:905-15. 2005..These observations suggest that multiple proteins involved in human decapping are important subunits of PBs and are activated on ARE-mRNAs by the protein TTP...
- Binding and cleavage of CRISPR RNA by Cas6Jason Carte
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
RNA 16:2181-8. 2010..Finally, we show that P. furiosus Cas6 remains stably associated with its cleavage products, suggesting additional roles for Cas6 in psiRNA biogenesis...
- Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiationVerena Pfeiffer
Max Planck Institute for Infection Biology, RNA Biology Group, Berlin, Germany
Nat Struct Mol Biol 16:840-6. 2009..The discovery of CDS targeting markedly expands the sequence space for sRNA target predictions in bacteria...
- The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activitiesDaneen Schaeffer
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
Nat Struct Mol Biol 16:56-62. 2009..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
- A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNAG Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Science 293:834-8. 2001..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
- New insights into the role of RNase L in innate immunityArindam Chakrabarti
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Interferon Cytokine Res 31:49-57. 2011..These newly described findings about RNase L could eventually provide the basis for developing broad-spectrum antimicrobial drugs...
- Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNAKlas I Udekwu
Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
Genes Dev 19:2355-66. 2005..MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear...
- MicroRNA expression is required for pancreatic islet cell genesis in the mouseFrancis C Lynn
Diabetes Center, Hormone Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143 0534, USA
Diabetes 56:2938-45. 2007..We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development...
- Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosomeJunjun Gao
Institute for Cell and Molecular Biology, University of Texas, TX 78712, USA
Mol Microbiol 61:394-406. 2006..coli...
- Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegansR F Ketting
The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
Genes Dev 15:2654-9. 2001..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41)...
- A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeatsNatalia Beloglazova
Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
J Biol Chem 283:20361-71. 2008..Here we show that the CAS2 proteins represent a novel family of endoribonucleases. Six purified CAS2 proteins from diverse organisms cleaved single-stranded RNAs preferentially within U-rich ..
- Fine-tuning of the unfolded protein response: Assembling the IRE1alpha interactomeClaudio Hetz
The FONDAP Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago, Chile
Mol Cell 35:551-61. 2009..In this Review, we discuss recent evidence supporting a model where IRE1alpha signaling emerges as a highly regulated process, controlled by the formation of a dynamic scaffold onto which many regulatory components assemble...
- Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeastFeng He
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Cell 12:1439-52. 2003....
- RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAsTeppei Morita
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
Genes Dev 19:2176-86. 2005..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
- Interferon action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase LA Zhou
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, OH 44195, USA
EMBO J 16:6355-63. 1997..Another implication is that the 2-5A system is likely to contribute to the antiviral activity of interferon by inducing apoptosis of infected cells...
- Mammalian endoplasmic reticulum stress sensor IRE1 signals by dynamic clusteringHan Li
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
Proc Natl Acad Sci U S A 107:16113-8. 2010..Thus IRE1 activity is governed by a timer that may be important in switching the UPR from the initially cytoprotective phase to the apoptotic mode...
- Ribonuclease activity and RNA binding of recombinant human DicerPatrick Provost
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
EMBO J 21:5864-74. 2002..Availability of the recombinant Dicer protein will help improve our understanding of RNA silencing and other Dicer-related processes...
- Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammalsC Patil
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
Curr Opin Cell Biol 13:349-55. 2001..Recent results reveal that the output of the mammalian UPR reaches beyond transcriptional regulation of secretory pathway components to control of general translation, the cell cycle and programmed cell death...
- Divergent effects of PERK and IRE1 signaling on cell viabilityJonathan H Lin
Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA
PLoS ONE 4:e4170. 2009..These results demonstrate that extended PERK and IRE1 signaling have opposite effects on cell viability. Differential activation of PERK and IRE1 may determine life or death decisions after ER protein misfolding...
- PKR and RNase L contribute to protection against lethal West Nile Virus infection by controlling early viral spread in the periphery and replication in neuronsMelanie A Samuel
Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
J Virol 80:7009-19. 2006..Our data suggest that PKR and RNase L contribute to IFN-mediated protection in a cell-restricted manner and control WNV infection in peripheral tissues and some neuronal subtypes...
- Mutations in the RNA component of RNase MRP cause a pleiotropic human disease, cartilage-hair hypoplasiaM Ridanpaa
Folkhalsan Institute of Genetics, 00280 Helsinki, Finland
Cell 104:195-203. 2001..The association of protein subunits with RNA appears unaltered. We conclude that mutations in RMRP cause CHH by disrupting a function of RNase MRP RNA that affects multiple organ systems...
- Role of polynucleotide phosphorylase in sRNA function in Escherichia coliNicholas De Lay
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
RNA 17:1172-89. 2011....
- 3' end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNAJeremy E Wilusz
Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Cell 135:919-32. 2008..Our findings reveal a 3' end processing mechanism by which a single gene locus can yield both a stable nuclear-retained noncoding RNA with a short poly(A) tail-like moiety and a small tRNA-like cytoplasmic RNA...
- Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coliYoshiki Ikeda
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
Mol Microbiol 79:419-32. 2011..Finally, we demonstrate that overexpression of RhlB partially inhibits the Hfq binding to RNase E and the rapid degradation of ptsG mRNA...
- Human cytomegalovirus infection activates and regulates the unfolded protein responseJennifer A Isler
Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, USA
J Virol 79:6890-9. 2005..These results suggest that, although HCMV infection induces the unfolded protein response, it modifies the outcome to benefit viral replication...
- Of proteins and RNA: the RNase P/MRP familyOlga Esakova
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
RNA 16:1725-47. 2010..We review current information on RNase P and the closely related universal eukaryotic enzyme RNase MRP, focusing on their functions and structural organization...
- Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAiThomas A Volpe
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
Science 297:1833-7. 2002..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
- Endonucleolytic initiation of mRNA decay in Escherichia coliAgamemnon J Carpousis
Laboratoire de Microbiologie et Genetique Moleculaires, CNRS et Université Paul Sabatier, 31062 Toulouse, France
Prog Mol Biol Transl Sci 85:91-135. 2009..In E. coli, the turnover of mRNA involves multiple, redundant pathways involving 3'-exoribonucleases, endoribonucleases, and a variety of other enzymes that modify RNA covalently or affect its conformation...
- The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein responseJiahai Zhou
Life Sciences Institute, LSI 3163B, Department of Biological Chemistry, University of Michigan Medical Center, 210 Washtenaw Avenue, Ann Arbor, MI 48109, USA
Proc Natl Acad Sci U S A 103:14343-8. 2006..Our structural, biochemical, and functional studies in vivo altogether demonstrate that IRE1 and PERK have conserved a common molecular interface necessary and sufficient for dimerization and UPR signaling...
- Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins"Shahar Amitai
Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
PLoS Genet 5:e1000390. 2009....
- A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase LJian Qiu Han
Department of Microbiology, School of Medicine, University of Colorado, Mail Stop 8333, Room P18 9116, 12800 E 19th Ave, Aurora, CO 80045, USA
J Virol 81:5561-72. 2007....
- Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coliYi Chun Tsai
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
Nucleic Acids Res 40:10417-31. 2012....
- Antagonism of the interferon-induced OAS-RNase L pathway by murine coronavirus ns2 protein is required for virus replication and liver pathologyLing Zhao
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Cell Host Microbe 11:607-16. 2012..Thus, RNase L is a critical cellular factor for protection against viral infection of the liver and the resulting hepatitis...
- Two pathways for RNase E action in Escherichia coli in vivo and bypass of its essentiality in mutants defective for Rho-dependent transcription terminationK Anupama
Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 001, India
Mol Microbiol 82:1330-48. 2011....
- Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferaseJung Ho Park
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
FEBS Lett 585:2526-32. 2011..This cleavage site is over-represented in the B. subtilis genes associated with biosynthesis of secondary metabolites, suggesting that MazF-bs may be involved in the regulation of the production of secondary metabolites...
- Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNAYvonne Göpel
Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, D 37077 Gottingen, Germany
Genes Dev 27:552-64. 2013..Thus, in analogy to regulated proteolysis, RapZ is an adaptor, and GlmY is an anti-adaptor in regulated turnover of a regulatory small RNA...
- Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonucleaseSandy Mattijssen
Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
Cell Mol Life Sci 68:2469-80. 2011..This is the first study providing direct evidence for the cleavage of a mRNA by RNase MRP/RNase P in human cells. Implications for the involvement in the pathophysiology of CHH are discussed...
- The social fabric of the RNA degradosomeKatarzyna J Bandyra
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
Biochim Biophys Acta 1829:514-22. 2013..This article is part of a Special Issue entitled: RNA Decay mechanisms...
- Role of RNase MRP in viral RNA degradation and RNA recombinationHannah M Jaag
Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546 0091, USA
J Virol 85:243-53. 2011..To identify host endoribonucleases involved in degradation of Tomato bushy stunt virus (TBSV) in a Saccharomyces cerevisiae model host, we ..
- RNase L releases a small RNA from HCV RNA that refolds into a potent PAMPKrishnamurthy Malathi
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
RNA 16:2108-19. 2010..It is proposed that RNase L mediated cleavage of HCV RNA generates svRNA that activates RIG-I, thus propagating innate immune signaling to the IFN-β gene...
- RNase L plays a role in the antiviral response to West Nile virusSvetlana V Scherbik
Department of Biology, Georgia State University, P O Box 4010, Atlanta, Georgia 30302 4010, USA
J Virol 80:2987-99. 2006..The results suggest that RNase L activation is not a major component of the Oas1b-mediated flavivirus resistance phenotype...
- RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilisKaren Shahbabian
Institut de Biologie Physico Chimique, CNRS UPR9073, affiliated with Université de Paris 7 Denis Diderot, Paris, France
EMBO J 28:3523-33. 2009..This indicates that RNase Y might be not only important for riboswitch RNA turnover but also as a key player in the initiation of mRNA decay in B. subtilis. About 40% of the sequenced eubacterial species have an RNase Y orthologue...
- Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhBTaras Afonyushkin
Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter Dr Bohrgasse 9 4, A 1030 Vienna, Austria
Nucleic Acids Res 33:1678-89. 2005..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover...
- Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineageSergei B Koralov
Immune Disease Institute and Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Cell 132:860-74. 2008....
- Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complexChristelle M Roux
Department of Microbiology and Immunology, University of Rochester Medical Center, School of Medicine and Dentistry, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA
J Bacteriol 193:5520-6. 2011..Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms...
- Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'Annie Prud'homme-Généreux
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
Mol Microbiol 54:1409-21. 2004..These findings show that the RNA degradosome is a flexible macromolecular machine capable of adapting to altered environmental conditions...
- Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnoverAkio Yamashita
Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, Texas 77030, USA
Nat Struct Mol Biol 12:1054-63. 2005..Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay...
- Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiaeJe Hyun Yoon
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
J Cell Biol 189:813-27. 2010..These results demonstrate that Ste20 has an unexpected role in the modulation of mRNA decay and translation and that phosphorylation of Dcp2 is an important control point for mRNA decapping...
- Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAsIsabella Moll
Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
RNA 9:1308-14. 2003..It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack...
- Probing the initiation and effector phases of the somatic piRNA pathway in DrosophilaAstrid D Haase
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Genes Dev 24:2499-504. 2010..Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis...
- Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase EKazushi Suzuki
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
Genes Dev 20:2605-17. 2006..Thus, these studies demonstrate a novel process for the selective targeting of RNA molecules for degradation by RNase E and a novel function for a GGDEF-EAL protein...
- A microRNA in a multiple-turnover RNAi enzyme complexGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
Science 297:2056-60. 2002..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
- The making of tRNAs and more - RNase P and tRNase ZRoland K Hartmann
Philipps Universitat Marburg, Institut fur Pharmazeutische Chemie, Marbacher Weg 6, D 35037 Marburg, Germany
Prog Mol Biol Transl Sci 85:319-68. 2009..The current knowledge on prokaryotic tRNase Z in tRNA 3'-processing is reviewed here. A second, tRNase Z-independent pathway of tRNA 3'-end maturation involving 3'-exonucleases will also be discussed...
- A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral MicroRNAsHal P Bogerd
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
Mol Cell 37:135-42. 2010....
- Bypassing a kinase activity with an ATP-competitive drugFeroz R Papa
Department of Medicine, University of California, San Francisco, CA 94143 2200, USA
Science 302:1533-7. 2003..Thus, rather than through phosphorylation per se, a conformational change in the kinase domain triggered by occupancy of the active site with a ligand leads to activation of all known downstream functions...
- Identification of a consensus element recognized and cleaved by IRE1 alphaDaisuke Oikawa
Iwawaki Initiative Research Unit, Advanced Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
Nucleic Acids Res 38:6265-73. 2010..The sequence and structure were also conserved in the known IRE1α cleavage targets, CD59 and XBP1. These findings provide the important clue to understanding the molecular mechanisms by which IRE1α recognizes and cleaves target RNAs...
- The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBKMaria Belitsky
Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel Canada, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
Mol Cell 41:625-35. 2011..These findings are intriguing, since most known quorum-sensing molecules monitor gene expression on the transcriptional level, while EDF monitors posttranscriptionally...
- RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coliMartin Lehnik-Habrink
Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
J Bacteriol 193:5431-41. 2011..The results presented in this study provide novel evidence for the idea that RNase Y is the functional equivalent of RNase E, even though the two enzymes do not share any sequence similarity...
- ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeatsHideki Nishitoh
Laboratory of Cell Signaling, Graduate School, Tokyo Medical and Dental University, Bunkyo ku 113 8549, Japan
Genes Dev 16:1345-55. 2002..These findings suggest that ASK1 is a key element in ER stress-induced cell death that plays an important role in the neuropathological alterations in polyQ diseases...
- An RNA degradosome assembly in Caulobacter crescentusSteven W Hardwick
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
Nucleic Acids Res 39:1449-59. 2011..Finally, we observe that the abundance of RNase E varies through the cell cycle, with maxima at morphological differentiation and cell division. This variation may contribute to the program of gene expression during cell division...
- The RNA of RNase MRP is required for normal processing of ribosomal RNAS Chu
Department of Biology, University of Rochester, NY 14627
Proc Natl Acad Sci U S A 91:659-63. 1994..A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing...
- RNAi-mediated targeting of heterochromatin by the RITS complexAndre Verdel
Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
Science 303:672-6. 2004..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
- mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systemsYoshihiro Yamaguchi
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Prog Mol Biol Transl Sci 85:467-500. 2009..We propose that mRNA interferases play roles not only in cell growth regulation and programmed cell death, but also in regulation of specific gene expression (either positively or negatively) in bacteria...
- Influenza A viral replication is blocked by inhibition of the inositol-requiring enzyme 1 (IRE1) stress pathwayIhab H Hassan
Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 287:4679-89. 2012..Decreasing viral replication by modulating the host ER stress response is a novel strategy that has important therapeutic implications...
- From conformational chaos to robust regulation: the structure and function of the multi-enzyme RNA degradosomeMaria W Górna
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
Q Rev Biophys 45:105-45. 2012..Parallels are drawn with analogous machinery in organisms from all life domains. Finally, we conjecture on roles of the degradosome as a regulatory hub for complex cellular processes...
- On the role of RNA amplification in dsRNA-triggered gene silencingT Sijen
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Cell 107:465-76. 2001..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
- Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coliSergio Martinez Rodriguez
Laboratorium voor Ultrastructuur, Vrije Universiteit Brussels, Pleinlaan 2, B 1050 Brussels, Belgium
Protein Sci 17:681-90. 2008..This B1 base recognition site is highly variable among bacterial sequences and the observed interactions are unique to EcRNaseI and a few close relatives...
- Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursorA Oguro
Institute of Biological Sciences, University of Tsukuba, Tsukuba shi, Ibaraki 305, Japan
J Biol Chem 273:19542-7. 1998..It is concluded that Bs-RNase III is an enzyme that processes pre-scRNA...
- RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming abilityMasaru Tamura
Stanford University School of Medicine, Department of Genetics, 300 Pasteur Drive, Stanford, CA 94305 5120, USA
J Bacteriol 188:5145-52. 2006..Our results demonstrate that RNase E activity is required to maintain a proper cellular ratio of the FtsZ and FtsA proteins in E. coli but that FtsZ deficiency does not account for the nonviability of cells lacking RNase E...
- HDLs inhibit endoplasmic reticulum stress and autophagic response induced by oxidized LDLsC Muller
Inserm U 858, Department of Vascular Biology, IFR 150, CHU Rangueil, Toulouse, France
Cell Death Differ 18:817-28. 2011..Finally, the cytosolic Ca(2+) deregulation triggered by oxLDLs is a common signaling pathway that mediates ER stress-induced cell death and autophagy, all these events being blocked by HDLs...
- Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiationWalter Rossmanith
Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria
PLoS ONE 6:e19152. 2011..Our data suggest that RNase Z(L) is the enzyme involved in both, nuclear and mitochondrial tRNA 3' end maturation...
- Reactivity epitopes of ribonuclease III substratesALLEN WHITALL NICHOLSON; Fiscal Year: 2010..dsRNA cleavage is catalyzed by members of the ribonuclease III (RNase III) family of endoribonucleases, which are highly conserved in bacterial and eukaryotic cells...
- Mechanisms of ribosomal reactions: peptide bond formation, peptide release and mRScott A Strobel; Fiscal Year: 2013..Although RelE is structurally similar to the general family of endoribonucleases, it does not contain any of the residues expected to be important for catalysis...
- Ribonuclease III mechanism of double-strand RNA cleavageALLEN NICHOLSON; Fiscal Year: 2003..Double-stranded (ds) RNA cleavage is carried out by members of the ribonuclease Ill superfamily of endoribonucleases. RNase III orthologues participate in mRNA and rRNA maturation, mRNA degradation, and antisense RNA action...
- Role of Phospholipid Scramblase in Interferon ActionRobert Silverman; Fiscal Year: 2005..abstract_text> ..
- Role of RNase L in the Biology of Prostate CancerRobert Silverman; Fiscal Year: 2008..The experiments described in this PROPOSAL describe a previously unexplored area of research that may bring us closer to the eventual goal of understanding a molecular basis for prostate cancer. ..
- High Throughput Chemical Genomics to Identify Novel Inhibitors of CHOPRandal Kaufman; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- The Role of CXCL9 in Genital HSV-2 InfectionDaniel Carr; Fiscal Year: 2009..g., vaccine or gene transfer) to reduce the incidence of infection or reduce the capacity of latent virus to reactivate. ..
- Unfolded proteins in the Endoplasmic Reticulum to DiseaseRandal Kaufman; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Unfolded Protein Response in Glucose HomeostasisRandal Kaufman; Fiscal Year: 2006..These in vivo models have the potential for providing novel biologic insights into human disease and may also facilitate testing of new therapies. [unreadable] [unreadable] [unreadable]..
- mazEF-mediated programmed cell death network in E. coliHanna Engelberg Kulka; Fiscal Year: 2010..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
- INITIATION OF MRNA DECAY IN BACILLUS SUBTILISDavid H Bechhofer; Fiscal Year: 2010..The likely participation of 2 B. subtilis endoribonucleases -- RNase J1 and RNase J2 -in mRNA decay will be assessed...
- Beta-globin mRNA decay in erythroid cellsDaniel Schoenberg; Fiscal Year: 2005..The long-term goal is to identify new therapeutic targets for treating beta-thalassemia and other diseases caused by PTCs by better understanding the enzymatic mechanisms responsible for the degradation of PTC-containing mRNAs. ..
- Anti-Viral Gene Delivery in the Nervous SystemDaniel Carr; Fiscal Year: 2005..abstract_text> ..
- REGULATED POLYADENYLATION OF MESSENGER RNADaniel Schoenberg; Fiscal Year: 2004....
- COORDINATION OF BACTERIAL RIBOSOMAL PROTEIN SYNTHESISLasse Lindahl; Fiscal Year: 2001....
- TRANSLATIONAL CONTROL IN REGULATION OF APOPTOSISRandal Kaufman; Fiscal Year: 2002....
- NEUROIMMUNOLOGY OF AED IN OCULAR HSV-1 INFECTIONDaniel Carr; Fiscal Year: 2001..abstract_text> ..
- Conformational Diseases of the Secretory PathwayRandal Kaufman; Fiscal Year: 2003..This conference will focus on new technologies and methodologies to study this rapidly moving field in order to dissect the complexity and diversity of ER function. ..
- Increasing 2-5A effectiveness through better chemistryHagen Cramer; Fiscal Year: 2003..Ridgeway Biosystems Inc. will apply successful chemical modifications to other therapeutics currently under development. ..
- A FADD-Associated Helicase Essential for Host DefenseGlen Barber; Fiscal Year: 2008..We anticipate that these studies will improve our knowledge of pathogenesis and provide new concepts for the rational design of novel therapeutic and vaccine strategies. [unreadable] [unreadable] [unreadable]..
- Role of the Unfolded Protein Response in Type 2 DiabetesFEROZ PAPA; Fiscal Year: 2008..The UCSF Department of Medicine is fully committed to Dr. Papa's career development, and will make all necessary resources available to facilitate the success of the project. ..
- Role of PKR in Growth RegulationGlen Barber; Fiscal Year: 2008..We propose that these aims will lend insight into mechanisms of cell signaling, tumorigenesis and interferon-mediated actions in the cell. ..
- Molecular identification of a sialyl 9-O-acetyl transferaseShiv Pillai; Fiscal Year: 2008..The gene that codes for an enzyme that controls the strength of the immune response will be studied. This gene may be an important target for the treatment of lupus and other related diseases. [unreadable] [unreadable] [unreadable]..
- Targeting RNA to develop novel antibacterial agentsCHRISTOPHER TROTTA; Fiscal Year: 2003..Broad-spectrum inhibitors will be further tested for inhibition of the eucaryal RNase P enzyme and cytotoxicity against a panel of mammalian cell lines. ..
- Estrogen regulation of uterine microRNAs: Novel factors in MMP-9 regulationWarren Nothnick; Fiscal Year: 2008..Enhancing our understanding on the regulation of MMP-9 expression may lead to the establishment of novel approaches to counteract mis-expression of this protease and the diseases associated with it. [unreadable] [unreadable] [unreadable]..
- MECHANISM OF INTERFERON ACTIONCHARLES SAMUEL; Fiscal Year: 2009..Furthermore, elucidation of the actions of IFN at the molecular level is of importance in view of potential applications of interferon in the clinic. ..
- Signal Transduction by Histidine Kinases and their Response RegulatorsMasayori Inouye; Fiscal Year: 2009..abstract_text> ..
- Catalytic Mechanisms of RNA EzymesMartha J Fedor; Fiscal Year: 2010..The results of these studies also will provide a framework to guide the development of technical and therapeutic applications involving RNAs as targets and reagents. ..
- Mechanisms of VSV-mediated OncolysisGlen N Barber; Fiscal Year: 2010..Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
- Impact of the interferon regulated proteins XAF1 and ZNF313 on innate immunityDouglas W Leaman; Fiscal Year: 2010....
- Targeted Therapy for Burkitt Lymphoma in Resource Poor SettingsGlen N Barber; Fiscal Year: 2010..Our long term goal is to develop a targeted, efficacious, antiviral-based therapy for EBV (HIV) associated lymphoma that is applicable in developing nations. ..
- 2007 Epigenetics Gordon Research ConferenceSteven Jacobsen; Fiscal Year: 2007..Perhaps equally importantly, it will launch the careers of new scientists in this frontier area. [unreadable] [unreadable] [unreadable]..
- Innate Intracellular MechanismsGlen N Barber; Fiscal Year: 2010..The importance of STING in innate signaling will be determined. Understanding how these pathways function has significant impact on understanding pathogenesis and for developing novel therapeutics and vaccines to combat disease. ..
- Role of the Yeast Lsm Proteins in mRNA DecayTHARUN SUNDARESAN; Fiscal Year: 2010..abstract_text> ..
- mTOR Signaling In Chronic Myelogenous LeukemiaHung Fan; Fiscal Year: 2009..abstract_text> ..
- MEMBRANE IMMUNOGLOBULIN ASSEMBLY AND FUNCTIONShiv Pillai; Fiscal Year: 2002..Since the majority of human lymphomas develop form activated B-cells, these signaling mechanisms are likely to be of direct relevance for a significant proportion of human cancers. ..
- The role of PKK in NFkappa B activationShiv Pillai; Fiscal Year: 2007..PKK represents a novel enzyme that may be critical in pathways that are important in cancer, inflammation, cell survival, and differentiation. ..
- Nitrosylcobalamin - A Novel Chemotherapeutic AgentDaniel Lindner; Fiscal Year: 2007..Determine whether inhibition of TCII-R expression causes resistance to NO-Cbl d. Define effects of IFN-beta upon S-nitrosylation of TRAIL receptors and PRL phosphatase ..
- Molecular Mediators of Interferon induced ApoptosisDouglas Leaman; Fiscal Year: 2005..The results of these studies should provide important information on the molecular details of antitumor activity of IFNs that can be used to identify or develop targeted therapeutics that augment IFN effects. ..
- 2-5A Antisense for Degradation of SARS Coronavirus RNAHagen Cramer; Fiscal Year: 2004..We propose to develop an effective and marketable 2-5A antisense drug for the treatment of SARS infection. ..
- REVERSE TRANSCRIPTASES IN THE PROKARYOTESMasayori Inouye; Fiscal Year: 2004....
- Viral DNA Replication and Late TranscriptionLinda Guarino; Fiscal Year: 2004..Knowledge gained from these experiments should be relevant to other viral systems and will provide a better understanding of the complex interactions between transcription and replication machinery in eukaryotic cells. ..
- Cold Shock Vectors for Single Protein ProductionMasayori Inouye; Fiscal Year: 2004..The proposed vector-host system will be highly useful not only for structural biology but also for medicinal research involving human proteins, which can not be obtained by conventional expression systems. ..
- Screening for Rnase L ActivatorsHagen Cramer; Fiscal Year: 2004..The successful completion of this proposed project will open a whole new class of therapy for currently intractable cancer and viral infections leveraging an important pathway of the body's natural immunity. ..
- Marginal zone B lymphocytes and blood borne pathogensShiv Pillai; Fiscal Year: 2004..Finally we will attempt to determine whether antigen-specific MZ B cells, as opposed to follicuar B cells, can readily capture and present blood borne antigens, and thus activate na'fve T cells. ..
- FUNCTION ANALYSIS OF THE DSRNA REGULATED NUCLEAR FACTORSGlen Barber; Fiscal Year: 2004..abstract_text> ..
- Studies on human peripheral B lymphocytesShiv Pillai; Fiscal Year: 2005....
- EXPRESSION AND PATHOGENESIS OF MURINE LEUKEMIA VIRUSHung Fan; Fiscal Year: 2005..low expression. Together, these experiments will provide new and important insights into leukemogenesis by MuLVs. ..
- Tat-Driven Delivery of 2-5A Cargo for HIV RNA AblationPAUL TORRENCE; Fiscal Year: 2003..This Tat lector-driven transport of 2-5A cargo would provide a major advantage over standard antisense nucleiccid strategies that suffer from inefficient cellular uptake, ..
- STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLIMasayori Inouye; Fiscal Year: 2003..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
- Inositol hexakisphosphate kinase 2:apoptosis regulatorDaniel Lindner; Fiscal Year: 2006..Determine the relevance of IP6K2 domains for IFN- beta-therapy in vivo - Assess IP6K2 mutants grownas xenografts. ..
- The Role of TIMP-1 in Uterine PhysiologyWarren Nothnick; Fiscal Year: 2005..These studies will broaden our understanding on the role of TIMP-1 in uterine physiology and may help to develop new strategies to treat uterine disorders such as infertility, endometriosis and uterine cancer. ..
- MOLECULAR BIOLOGY OF JAAGSIEKTE SHEEP RETROVIRUSHung Fan; Fiscal Year: 2003..These experiments will provide insight into JSRV-induced lung cancer in sheep, and possibly into human BAC. ..
- Nucleic Acids Gordon Research Conference 2005MARTHA FEDOR; Fiscal Year: 2005..Considerable time is allotted for discussion following formal lectures. Poster sessions and optional recreational activities will stimulate informal interactions. ..
- PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTIONRichard Carthew; Fiscal Year: 2005..abstract_text> ..
- Novel Targets for Treatment of SmallpoxStewart Shuman; Fiscal Year: 2003....
- The Mammalian Signal Recognition ParticleKEVIN WEEKS; Fiscal Year: 2006..Moreover, the work is designed to identify principles generalizable to other biologically and medically prominent ribonucleoprotein complexes. ..
- Protein-Facilitates RNA CatalystsKEVIN WEEKS; Fiscal Year: 2007..5) To begin to explore the molecular mechanism by which Mrs1 facilitates catalysis of the bl3 group I intron. (6) To develop a high resolution model for the maturase-bl3 RNA complex. ..
- Proteomics of Cerebrospinal Fluid in Chronic Fatigue SyndromeJames Baraniuk; Fiscal Year: 2009..These methods and biomarkers may be of diagnostic value. They will be useful for assessing longitudinal changes in disease severity, phenotype, or the effects of treatment. ..
- Age and Sleep Effects on ER Protein FunctionNirinjini Naidoo; Fiscal Year: 2005..We will use 2-D DIGE, as well as oxidation assays and mass spectrometry, to identify proteins that are altered by aging and sleep deprivation in the ER. ..