phospholipases a

Summary

Summary: Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates. Phospholipase A1(EC 3.1.1.32) hydrolyzes the acyl group attached to the 1-position and phospholipase A2 (EC 3.1.1.4) hydrolyzes the acyl group attached to the 2-position.

Top Publications

  1. ncbi Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A(2)
    Juliana Pavan Zuliani
    Laboratorio de Farmacologia, Instituto Butantan, Av Vital Brazil, 1500 CEP 05503 900 Sao Paulo, SP, Brazil
    Toxicon 46:523-32. 2005
  2. ncbi The expanding superfamily of phospholipase A(2) enzymes: classification and characterization
    D A Six
    Department of Chemistry and Biochemistry, MC 0601, Revelle College and School of Medicine, University of California, San Diego, La Jolla, CA 92093 0601, USA
    Biochim Biophys Acta 1488:1-19. 2000
  3. ncbi Role of cytosolic phospholipase A2 in allergic response and parturition
    N Uozumi
    Department of Biochemistry, University of Tokyo, Japan
    Nature 390:618-22. 1997
  4. ncbi Biochemical and biological properties of phospholipases A(2) from Bothrops atrox snake venom
    Milton M Kanashiro
    Laboratorio de Biologia do Reconhecer, Centro de Biociências e Biotecnologia LBR, Universidade Estadual do Norte Fluminense, Av Alberto Lamego, 2000, Campos dos Goytacazes, Rio de Janeiro, Brazil
    Biochem Pharmacol 64:1179-86. 2002
  5. ncbi cPLA2 is phosphorylated and activated by MAP kinase
    L L Lin
    Genetics Institute, Small Molecule Drug Discovery Group, Cambridge, Massachusetts 02140
    Cell 72:269-78. 1993
  6. ncbi Bactericidal and neurotoxic activities of two myotoxic phospholipases A2 from Bothrops neuwiedi pauloensis snake venom
    Veridiana M Rodrigues
    Instituto de Genética e Bioquímica, Universidade Federal de Uberlandia, UFU, Uberlandia, MG, Brazil
    Toxicon 44:305-14. 2004
  7. ncbi Inhibitors of prostaglandin synthesis inhibit human prostate tumor cell invasiveness and reduce the release of matrix metalloproteinases
    F A Attiga
    Department of Biology, George Washington University, Washington, DC 20037, USA
    Cancer Res 60:4629-37. 2000
  8. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
  9. ncbi Specific physiological roles of cytosolic phospholipase A(2) as defined by gene knockouts
    A Sapirstein
    Department of Anesthesiology and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Biochim Biophys Acta 1488:139-48. 2000
  10. ncbi Chemical modifications of phospholipases A2 from snake venoms: effects on catalytic and pharmacological properties
    Andreimar M Soares
    Departamento de Biotecnologia, Universidade de Ribeirão Preto UNAERP, Ribeirão Presto, SP, Brazil
    Toxicon 42:855-68. 2003

Research Grants

  1. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Alex Brown; Fiscal Year: 2010
  2. 48th Annual South Eastern Regional Lipid Conference (SERLC) Funding Support
    Carole A Oskeritzian; Fiscal Year: 2013
  3. LUNG INFLAMMATION
    Gerald Weissmann; Fiscal Year: 1999
  4. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Andrew Morris; Fiscal Year: 2004
  5. South Eastern Region Lipid Conference 2006
    Charles Chalfant; Fiscal Year: 2007
  6. CDP-choline: Mechanisms in Cerebral Ischemia
    RAO ADIBHATLA; Fiscal Year: 2006
  7. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
  8. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
  9. BIOCHEMICAL STUDIES OF 14 KDA PHOSPHOLIPASES A2
    Michael Gelb; Fiscal Year: 2006
  10. METABOLISM OF PLATELET-ACTIVATING FACTOR
    Diana Stafforini; Fiscal Year: 2008

Detail Information

Publications243 found, 100 shown here

  1. ncbi Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A(2)
    Juliana Pavan Zuliani
    Laboratorio de Farmacologia, Instituto Butantan, Av Vital Brazil, 1500 CEP 05503 900 Sao Paulo, SP, Brazil
    Toxicon 46:523-32. 2005
    ..It is concluded that, at non-cytotoxic concentrations, MT-II and MT-III activate defense mechanisms in macrophages up regulating phagocytosis, mainly via mannose and beta-glucan receptors, and the respiratory burst...
  2. ncbi The expanding superfamily of phospholipase A(2) enzymes: classification and characterization
    D A Six
    Department of Chemistry and Biochemistry, MC 0601, Revelle College and School of Medicine, University of California, San Diego, La Jolla, CA 92093 0601, USA
    Biochim Biophys Acta 1488:1-19. 2000
    ..These four criteria have led to the expansion or realignment of Groups VI, VII and VIII, as well as the addition of Group XI PLA(2) from plants...
  3. ncbi Role of cytosolic phospholipase A2 in allergic response and parturition
    N Uozumi
    Department of Biochemistry, University of Tokyo, Japan
    Nature 390:618-22. 1997
    ..Considered together with previous findings, our results indicate that cytosolic PLA2 plays a non-redundant role in allergic responses and reproductive physiology...
  4. ncbi Biochemical and biological properties of phospholipases A(2) from Bothrops atrox snake venom
    Milton M Kanashiro
    Laboratorio de Biologia do Reconhecer, Centro de Biociências e Biotecnologia LBR, Universidade Estadual do Norte Fluminense, Av Alberto Lamego, 2000, Campos dos Goytacazes, Rio de Janeiro, Brazil
    Biochem Pharmacol 64:1179-86. 2002
    b>Phospholipases A(2) (PLA(2)s), of molecular mass 13-15kDa, are commonly isolated from snake venom...
  5. ncbi cPLA2 is phosphorylated and activated by MAP kinase
    L L Lin
    Genetics Institute, Small Molecule Drug Discovery Group, Cambridge, Massachusetts 02140
    Cell 72:269-78. 1993
    ..Taken together, these data indicate that MAP kinase mediates, at least in part, the agonist-induced activation of cPLA2...
  6. ncbi Bactericidal and neurotoxic activities of two myotoxic phospholipases A2 from Bothrops neuwiedi pauloensis snake venom
    Veridiana M Rodrigues
    Instituto de Genética e Bioquímica, Universidade Federal de Uberlandia, UFU, Uberlandia, MG, Brazil
    Toxicon 44:305-14. 2004
    ..In addition, BnpTX-I displays a heparin binding C-terminal region, which is probably responsible for the cytotoxic and bactericidal effects...
  7. ncbi Inhibitors of prostaglandin synthesis inhibit human prostate tumor cell invasiveness and reduce the release of matrix metalloproteinases
    F A Attiga
    Department of Biology, George Washington University, Washington, DC 20037, USA
    Cancer Res 60:4629-37. 2000
    ....
  8. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
    ....
  9. ncbi Specific physiological roles of cytosolic phospholipase A(2) as defined by gene knockouts
    A Sapirstein
    Department of Anesthesiology and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Biochim Biophys Acta 1488:139-48. 2000
    ..The importance of cPLA(2) in inflammation and tissue injury suggests that pharmacological targeting of this enzyme may have important therapeutic benefits...
  10. ncbi Chemical modifications of phospholipases A2 from snake venoms: effects on catalytic and pharmacological properties
    Andreimar M Soares
    Departamento de Biotecnologia, Universidade de Ribeirão Preto UNAERP, Ribeirão Presto, SP, Brazil
    Toxicon 42:855-68. 2003
    ....
  11. ncbi The phospholipase A2 superfamily and its group numbering system
    Ralph H Schaloske
    Department of Pharmacology, School of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0601, USA
    Biochim Biophys Acta 1761:1246-59. 2006
    ..Since our last update, a number of new PLA(2)s have been discovered and are now included. Additionally, tools for the investigation of PLA(2)s and approaches for distinguishing between the different Groups are described...
  12. ncbi Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis
    Lucimara Chioato
    Department of Biochemistry and Immunology, FMRP USP, Universidade de Sao Paulo, Brazil
    Toxicon 42:869-83. 2003
    ....
  13. ncbi Bactericidal properties of human and murine groups I, II, V, X, and XII secreted phospholipases A(2)
    Rao S Koduri
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 277:5849-57. 2002
    ....
  14. pmc Molecular identification of cytosolic, patatin-related phospholipases A from Arabidopsis with potential functions in plant signal transduction
    André Holk
    Universitat Hannover, Institut für Zierpflanzenbau, Baumschule und Pflanzenzuchtung, Herrenhauser Strasse 2, D 30419 Hannover
    Plant Physiol 130:90-101. 2002
    ....
  15. ncbi In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholipase A2 inhibitors
    Rebecca M Phillips
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093 0314, USA
    J Biol Chem 278:41326-32. 2003
    ....
  16. ncbi Arachidonic acid release from mammalian cells transfected with human groups IIA and X secreted phospholipase A(2) occurs predominantly during the secretory process and with the involvement of cytosolic phospholipase A(2)-alpha
    Carine M Mounier
    Departments of Chemistry and Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:25024-38. 2004
    Stable expression of human groups IIA and X secreted phospholipases A(2) (hGIIA and hGX) in CHO-K1 and HEK293 cells leads to serum- and interleukin-1beta-promoted arachidonate release...
  17. ncbi Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis
    Youbong Hyun
    National Research Laboratory of Plant Developmental Genetics, Department of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Dev Cell 14:183-92. 2008
    ..Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression...
  18. ncbi Cellular regulation and proposed biological functions of group VIA calcium-independent phospholipase A2 in activated cells
    Jesús Balsinde
    Eicosanoid Research Division, Institute of Molecular Biology and Genetics, University of Valladolid School of Medicine, Avenida Ramón y Cajal 7, 47005 Valladolid, Spain
    Cell Signal 17:1052-62. 2005
    ..This review focuses on the biochemical mechanisms that regulate the activity of iPLA2-VIA in activated cells, and the biological functions proposed for this enzyme during stimulus-response coupling...
  19. ncbi Cross-talk between cytosolic phospholipase A2 alpha (cPLA2 alpha) and secretory phospholipase A2 (sPLA2) in hydrogen peroxide-induced arachidonic acid release in murine mesangial cells: sPLA2 regulates cPLA2 alpha activity that is responsible for arachido
    Won K Han
    Medical Services, Massachusetts General Hospital, Department of Medicine and Anesthesia, Harvard Medical School, Massachusetts, USA
    J Biol Chem 278:24153-63. 2003
    ..Group IIa and V PLA2s are regulatory and cPLA2alpha is responsible for AA release...
  20. ncbi Fcgamma RI-triggered generation of arachidonic acid and eicosanoids requires iPLA2 but not cPLA2 in human monocytic cells
    Hwee Kee Tay
    Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore 117597
    J Biol Chem 279:22505-13. 2004
    ....
  21. ncbi Inhibition of snake venoms and phospholipases A(2) by extracts from native and genetically modified Eclipta alba: isolation of active coumestans
    Luciana C Diogo
    Department of Biotechnology, University of Ribeirao Preto, Ribeirao Preto, Sao Paulo, Brazil
    Basic Clin Pharmacol Toxicol 104:293-9. 2009
    ..coumestans (wedelolactone and demethylwedelolactone) inhibited the myotoxic activity induced by basic phospholipases A(2) isolated from the venoms of Crotalus durissus terrificus (CB) and Bothrops jararacussu (BthTX-I and II)...
  22. ncbi Inflammatory effects of snake venom myotoxic phospholipases A2
    C F P Teixeira
    Laboratories of Pharmacology, Instituto Butantan, 05503 900 Sao Paulo, SP, Brazil
    Toxicon 42:947-62. 2003
    ..This work reviews the proinflammatory and nociceptive effects evoked by myotoxic PLA2s and their mechanisms of action...
  23. pmc Inhibition of mitochondrial calcium-independent phospholipase A2 (iPLA2) attenuates mitochondrial phospholipid loss and is cardioprotective
    Scott D Williams
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, U S A
    Biochem J 362:23-32. 2002
    ..Taken together, these data strongly implicate mitochondria-associated iPLA(2) in the signal transduction of myocardial I/R injury...
  24. ncbi The molecular biology of the group VIA Ca2+-independent phospholipase A2
    Z Ma
    Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Prog Nucleic Acid Res Mol Biol 67:1-33. 2001
    ..Further study of its regulation and interaction with other proteins may yield insight into how its structural features are related to its function...
  25. ncbi Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s
    N Suzuki
    Shionogi Research Laboratories, Shionogi and Company, Limited, Sagisu 5 12 4, Fukushima ku, Osaka 553 0002, Japan
    J Biol Chem 275:5785-93. 2000
    ..These findings suggest a distinct functional role of novel sPLA(2)-IIE in the progression of inflammatory processes...
  26. ncbi p38 mitogen-activated protein kinase phosphorylates cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. Evidence that proline-directed phosphorylation is not required for mobilization of arachidonic acid by cPLA2
    R M Kramer
    Lilly Research Laboratories, Indianapolis, Indiana 46285, USA
    J Biol Chem 271:27723-9. 1996
    ....
  27. ncbi Secreted bacterial phospholipase A2 enzymes: better living through phospholipolysis
    Izabela Sitkiewicz
    Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, TX 77030, USA
    Trends Microbiol 15:63-9. 2007
    ....
  28. pmc Cytosolic phospholipase A2alpha-deficient mice are resistant to collagen-induced arthritis
    Martin Hegen
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Exp Med 197:1297-302. 2003
    ..These data demonstrate the critical role of cPLA2alpha in the pathogenesis of CIA...
  29. ncbi Groups IV, V, and X phospholipases A2s in human neutrophils: role in eicosanoid production and gram-negative bacterial phospholipid hydrolysis
    Norbert Degousee
    Division of Vascular Surgery, Max Bell Research Center, Toronto General Hospital, University Health Network, Toronto M5G 2C4, Canada
    J Biol Chem 277:5061-73. 2002
    ....
  30. ncbi Phospholipase A(2) regulation of arachidonic acid mobilization
    Jesús Balsinde
    Institute of Molecular Biology and Genetics, Spanish Council for Scientific Research, University of Valladolid School of Medicine, 47005, Valladolid, Spain
    FEBS Lett 531:2-6. 2002
    ..Multiple PLA(2) enzymes are active within and surrounding the cell and these enzymes have distinct, but interconnected roles in AA release...
  31. ncbi Type IIA secretory phospholipase A2 up-regulates cyclooxygenase-2 and amplifies cytokine-mediated prostaglandin production in human rheumatoid synoviocytes
    M J Bidgood
    Department of Medicine, University of New South Wales, and Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, New South Wales, Australia
    J Immunol 165:2790-7. 2000
    ..Therefore, sPLA2-IIA may be a critical modulator of cytokine-mediated synovial inflammation in RA...
  32. ncbi On the diversity of secreted phospholipases A(2). Cloning, tissue distribution, and functional expression of two novel mouse group II enzymes
    E Valentin
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS UPR 411, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
    J Biol Chem 274:31195-202. 1999
    Over the last decade, an expanding diversity of secreted phospholipases A(2) (sPLA(2)s) has been identified in mammals...
  33. ncbi Skeletal muscle degeneration induced by venom phospholipases A2: insights into the mechanisms of local and systemic myotoxicity
    Jose Maria Gutierrez
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    Toxicon 42:915-31. 2003
    ..In contrast, PLA2s that bind muscle cells in a more selective way are not sequestered by non-specific interactions with other cells and, consequently, are systemically distributed and reach muscle cells in other locations...
  34. ncbi Comparative structural studies on Lys49-phospholipases A(2) from Bothrops genus reveal their myotoxic site
    Juliana I dos Santos
    Depto de Física e Biofísica, Instituto de Biociencias, UNESP, SP, Brazil
    J Struct Biol 167:106-16. 2009
    b>Phospholipases A(2) (PLA(2)s) are membrane-associated enzymes that hydrolyze phospholipids at the sn-2 position, releasing lysophospholipids and free fatty acids...
  35. ncbi Inhibition of transferrin recycling and endosome tubulation by phospholipase A2 antagonists
    P de Figueiredo
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Biol Chem 276:47361-70. 2001
    ..When taken together with previous studies on the Golgi complex, these results also indicate that an intracellular PLA(2) activity provides a novel molecular mechanism for inducing tubule formation from multiple organelles...
  36. pmc Role of group V phospholipase A2 in zymosan-induced eicosanoid generation and vascular permeability revealed by targeted gene disruption
    Yoshiyuki Satake
    Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:16488-94. 2004
    ....
  37. ncbi Selective inhibitors of cytosolic or secretory phospholipase A2 block TNF-induced activation of transcription factor nuclear factor-kappa B and expression of ICAM-1
    L Thommesen
    UNIGEN Center for Molecular Biology, Department of Physiology and Biomedical Engineering, Norwegian University of Science and Technology, Trondheim
    J Immunol 161:3421-30. 1998
    ..The results indicate that both cPLA2 and sPLA2 may be involved in the TNF signal transduction pathway leading to nuclear translocation of NF-kappaB and to NF-kappaB-activated gene expression in HaCaT cells...
  38. ncbi The genomic organization, complete mRNA sequence, cloning, and expression of a novel human intracellular membrane-associated calcium-independent phospholipase A(2)
    D J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Chemistry and Molecular Biology, and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:9937-45. 2000
    ..to the ATP and lipase consensus sequences present in the founding members of a family of calcium-independent phospholipases A(2). Detailed inspection of the identified sequence (residues 79, 671-109,912 GenBank accession no...
  39. pmc Patatin-related phospholipase pPLAIIIβ-induced changes in lipid metabolism alter cellulose content and cell elongation in Arabidopsis
    Maoyin Li
    Department of Biology, University of Missouri, St Louis, Missouri 63121, USA
    Plant Cell 23:1107-23. 2011
    ..These data suggest that alteration of pPLAIIIβ expression and the resulting lipid changes alter cellulose content and cell elongation in Arabidopsis...
  40. ncbi Modulation of the susceptibility of human erythrocytes to snake venom myotoxic phospholipases A(2): role of negatively charged phospholipids as potential membrane binding sites
    C Diaz
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    Arch Biochem Biophys 391:56-64. 2001
    Cerrophidion (Bothrops) godmani myotoxins I (CGMT-I) and II (CGMT-II), Asp-49 and Lys-49 phospholipases A(2) (PLA2s), which drastically differ in enzymatic activity, were devoid of direct hemolytic effects on erythrocytes (RBC) from ..
  41. pmc Purification and characterization of a low-molecular-weight phospholipase A2 from developing seeds of elm
    U Stahl
    Department of Plant Biology, P O Box 7080, Swedish University of Agricultural Sciences, S 750 07 Uppsala, Sweden
    Plant Physiol 117:197-205. 1998
    ..The biochemical data and amino acid sequence alignments indicate that the enzyme is related to the well-characterized family of animal secretory PLA2s and, to our knowledge, is the first plant enzyme of this type to be described...
  42. ncbi A natural disruption of the secretory group II phospholipase A2 gene in inbred mouse strains
    B P Kennedy
    Department of Biochemistry and Molecular Biology, Merck Frosst Center for Therapeutic Research, Pointe Claire Dorval, Quebec, Canada
    J Biol Chem 270:22378-85. 1995
    ..The identification of this mutation should not only help define the physiological role of sPLA2 but also has important implications in mouse inflammatory models developed by targeted mutagenesis...
  43. ncbi The manifold phospholipases A of Legionella pneumophila - identification, export, regulation, and their link to bacterial virulence
    Sangeeta Banerji
    Research Group Pathogenesis of Legionella Infections, Robert Koch Institut, Nordufer 20, D 13353 Berlin, Germany
    Int J Med Microbiol 298:169-81. 2008
    ..Moreover, the regulation of lipolytic activities depends on the transcriptional regulators LetA/S and RpoS, inducing the expression of virulence traits, and on posttranscriptional activators like the zinc metalloprotease ProA...
  44. ncbi Mice deficient in group IV cytosolic phospholipase A2 are resistant to MPTP neurotoxicity
    P Klivenyi
    Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston 02114, USA
    J Neurochem 71:2634-7. 1998
    ..These findings provide evidence that cPLA2 plays a role in MPTP neurotoxicity and suggest that cPLA2 may play a role in the development of Parkinson's disease in humans...
  45. ncbi Distinct arachidonate-releasing functions of mammalian secreted phospholipase A2s in human embryonic kidney 293 and rat mastocytoma RBL-2H3 cells through heparan sulfate shuttling and external plasma membrane mechanisms
    M Murakami
    Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Biol Chem 276:10083-96. 2001
    ....
  46. ncbi Secretory phospholipase A2 mediates cooperative prostaglandin generation by growth factor and cytokine independently of preceding cytosolic phospholipase A2 expression in rat gastric epithelial cells
    S Akiba
    Department of Pathological Biochemistry, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    J Biol Chem 276:21854-62. 2001
    ..Presumably, the preceding cPLA(2) expression is not involved in the PGE(2) generation, because of impairment of its hydrolytic activity in the stimulated cells...
  47. ncbi Phospolipase A2 and apoptosis
    Makoto Mark Taketo
    Department of Pharmacology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1585:72-6. 2002
    ..Moreover, new proteins that appear to control PLA(2)s are being discovered. Here, the roles of PLA(2)s in apoptosis are discussed by reviewing recent reports...
  48. ncbi Multiple splice variants of the human calcium-independent phospholipase A2 and their effect on enzyme activity
    P K Larsson
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S 171 77 Stockholm, Sweden
    J Biol Chem 273:207-14. 1998
    ..These results suggest that the ankyrin-iPLA2 sequence can function as a negative regulator of iPLA2 activity and that the alternative splicing of the iPLA2 gene can have a direct effect on the attenuation of enzyme activity...
  49. ncbi The highly selective production of 2-arachidonoyl lysophosphatidylcholine catalyzed by purified calcium-independent phospholipase A2gamma: identification of a novel enzymatic mediator for the generation of a key branch point intermediate in eicosanoid sig
    Wei Yan
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26669-79. 2005
    ....
  50. ncbi Inhibition of the complete set of mammalian secreted phospholipases A(2) by indole analogues: a structure-guided study
    Brian P Smart
    Departments of Chemistry and Biochemistry, University of Washington, Seattle, WA 98195, USA
    Bioorg Med Chem 12:1737-49. 2004
    Structure-guided design was employed in a search for potent and selective inhibitors of mammalian secreted phospholipases A(2) (sPLA(2)s)...
  51. ncbi Polycyclic aromatic hydrocarbons present in cigarette smoke cause endothelial cell apoptosis by a phospholipase A2-dependent mechanism
    Patricia K Tithof
    Department of Comparative Medicine, The University of Tennessee, Knoxville, Tennessee 37996 4543, USA
    FASEB J 16:1463-4. 2002
    ..These data suggest that 1-MA, B(a)P and PA induce apoptosis of endothelial cells by a mechanism that involves activation of these three distinct isoforms of PLA2...
  52. pmc Cytosolic phospholipase A2 and its mode of activation in human neutrophils by opsonized zymosan. Correlation between 42/44 kDa mitogen-activated protein kinase, cytosolic phospholipase A2 and NADPH oxidase
    I Hazan
    Infectious Disease Laboratory and Clinical Biochemistry Department, Faculty of Health Sciences, Ben Gurion University of the Negev and Soroka Medical Center, 84105 Beer Sheva, Israel
    Biochem J 326:867-76. 1997
    ..Whereas PMA stimulates ERKs activity through a PKC-dependent pathway, signal transduction stimulated by OZ involves tyrosine kinase activity leading to activation of ERKs via a PKC-independent pathway...
  53. pmc Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22
    Z Ma
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:9607-16. 1999
    ....
  54. pmc Roles of various phospholipases A2 in providing lysophospholipid acceptors for fatty acid phospholipid incorporation and remodelling
    Jesús Balsinde
    Instituto de Biologia y Genetica Molecular, CSIC, Facultad de Medicina, Universidad de Valladolid, C Ramón y Cajal 7, E 47005 Valladolid, Spain
    Biochem J 364:695-702. 2002
    ..The enzyme activity cleaves both PC and PE and is thus able to provide the lysoPC and lysoPE acceptors required for the fatty acid acylation reactions...
  55. ncbi Structural and functional characterization of basic PLA2 isolated from Crotalus durissus terrificus venom
    D G Oliveira
    Departamento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas UNICAMP, SP, Brasil
    J Protein Chem 21:161-8. 2002
    ....
  56. ncbi Group X secretory phospholipase A(2) induces potent productions of various lipid mediators in mouse peritoneal macrophages
    A Saiga
    Shionogi Research Laboratories, Shionogi and Co, Ltd, 12-4 Sagisu, 5-chome, Fukushima-ku, 553-0002, Osaka, Japan
    Biochim Biophys Acta 1530:67-76. 2001
    ..These events might be relevant to the progression of various pathological states, including chronic inflammation and atherosclerosis...
  57. ncbi Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights
    Angelo J Magro
    Departamento de Física e Biofísica, IB, UNESP, Botucatu SP, Brazil
    Biochem Biophys Res Commun 311:713-20. 2003
    b>Phospholipases A(2) are components of Bothrops venoms responsible for disruption of cell membrane integrity via hydrolysis of its phospholipids...
  58. ncbi Neuronal receptors for phospholipases A(2 )and beta-neurotoxicity
    I Krizaj
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia
    Biochimie 82:807-14. 2000
    Some phospholipases A(2) interrupt neuromuscular communication by blocking the release of neurotransmitter into the synaptic cleft. Despite numerous studies, the molecular mechanism of their action is still largely obscure...
  59. ncbi Cytosolic phospholipase A2 plays a key role in the pathogenesis of multiple sclerosis-like disease
    Athena Kalyvas
    Centre for Research in Neuroscience, The Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, Quebec H3G 1A4, Canada
    Neuron 41:323-35. 2004
    ..We provide evidence that cPLA2 is highly expressed in EAE lesions and show that blocking this enzyme leads to a remarkable reduction in the onset and progression of EAE...
  60. ncbi A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP
    J D Clark
    Genetics Institute, Inc, Cambridge, Massachusetts 02140
    Cell 65:1043-51. 1991
    ..We suggest that this homology delineates a Ca(2+)-dependent phospholipid-binding motif, providing a mechanism for the second messenger Ca2+ to translocate and activate cytosolic proteins...
  61. ncbi Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells
    S T Reddy
    Department of Biological Chemistry, UCLA Center for the Health Sciences, Los Angeles, California 90095 1570, USA
    J Biol Chem 272:13591-6. 1997
    ....
  62. ncbi Renal concentrating defect in mice lacking group IV cytosolic phospholipase A(2)
    P Downey
    Medical and Anesthesia Services, Massachusetts General Hospital, Charlestown, 02129, USA
    Am J Physiol Renal Physiol 280:F607-18. 2001
    ..Thus mice lacking cPLA(2) develop an age-related defect in renal concentration that may be related to abnormal trafficking and/or folding of AQP1 in the proximal tubule, implicating cPLA(2) in these processes...
  63. ncbi A pyrrolidine-based specific inhibitor of cytosolic phospholipase A(2)alpha blocks arachidonic acid release in a variety of mammalian cells
    F Ghomashchi
    Departments of Chemistry and Biochemistry, University of Washington, Seattle, WA 98195, USA
    Biochim Biophys Acta 1513:160-6. 2001
    ....
  64. ncbi Positive Darwinian selection in Vipera palaestinae phospholipase A2 genes is unexpectedly limited to the third exon
    D Kordis
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia
    Biochem Biophys Res Commun 251:613-9. 1998
    ..The positive Darwinian selection is surprisingly limited to the third exon, in contrast to other Viperidae PLA2 genes, where it is present in all mature protein coding exons...
  65. ncbi Phosphorylation and activation of cytosolic phospholipase A2 by 38-kDa mitogen-activated protein kinase in collagen-stimulated human platelets
    A G Borsch-Haubold
    Department of Pharmacology, University of Oxford, UK
    Eur J Biochem 245:751-9. 1997
    ..The present results demonstrate an important role for p38 MAP kinase in the regulation of cytosolic PLA2 activity in collagen-stimulated human platelets...
  66. pmc Cytosolic phospholipase A2-alpha is necessary for platelet-activating factor biosynthesis, efficient neutrophil-mediated bacterial killing, and the innate immune response to pulmonary infection: cPLA2-alpha does not regulate neutrophil NADPH oxidase activ
    Barry B Rubin
    Division of Vascular Surgery and Toronto General Hospital Research Institute of the University Health Network, University of Toronto, Toronto, Ontario M5G 2C4, Canada
    J Biol Chem 280:7519-29. 2005
    ..These studies identify a novel role for cPLA(2)-alpha in the regulation of neutrophil-mediated bacterial killing and the innate immune response to bacterial infection...
  67. ncbi Sequence comparison of outer membrane phospholipases A: implications for structure and for the catalytic mechanism
    R G Brok
    Department of Molecular Cell Biology, Utrecht University, The Netherlands
    Res Microbiol 149:703-10. 1998
    ..The presence of proteins homologous to OMPLA even in non-enterobacterial Gram-negative bacteria indicates an important physiological role of this enzyme...
  68. ncbi Human calcium-independent phospholipase A2 mediates lymphocyte proliferation
    A K Roshak
    Department of Immunology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA
    J Biol Chem 275:35692-8. 2000
    ..of fatty acids such as arachidonic acid requires sn-2-deacylation catalyzed by a class of enzymes known as phospholipases A(2) (PLA(2), EC )...
  69. ncbi Induction of distinct sets of secretory phospholipase A(2) in rodents during inflammation
    Katsuhiko Hamaguchi
    Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Tokyo 142 8555, Shinagawa, Japan
    Biochim Biophys Acta 1635:37-47. 2003
    ..These results indicate that distinct sPLA(2)s can participate in inflammatory diseases according to tissues, animal species, and types of inflammation...
  70. ncbi Expression profiles of Arabidopsis phospholipase A IIA gene in response to biotic and abiotic stresses
    Yoshihiro Narusaka
    Kobe University Graduate School of Science and Technology, 1 1 Rokkodai cho, Nada Ku, Kobe, 657 8501 Japan
    Plant Cell Physiol 44:1246-52. 2003
    ..In conclusion, the promoter activity is induced under these stresses...
  71. ncbi Bromoenol lactone promotes cell death by a mechanism involving phosphatidate phosphohydrolase-1 rather than calcium-independent phospholipase A2
    Lucia Fuentes
    Institute of Molecular Biology and Genetics, University of Valladolid School of Medicine, E 47005 Valladolid, Spain
    J Biol Chem 278:44683-90. 2003
    ....
  72. ncbi The crystal structure, mutagenesis, and activity studies reveal that patatin is a lipid acyl hydrolase with a Ser-Asp catalytic dyad
    Timothy J Rydel
    Monsanto Company, Chesterfield, Missouri 63017 1732, USA
    Biochemistry 42:6696-708. 2003
    ..Patatin does not display interfacial activation, a hallmark feature of lipases, and this is likely due to the fact that it lacks a flexible lid that can shield the active site...
  73. ncbi Purified group X secretory phospholipase A(2) induced prominent release of arachidonic acid from human myeloid leukemia cells
    K Hanasaki
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Sagisu 5 12 4, Fukushima ku, Osaka 553 0002, Japan
    J Biol Chem 274:34203-11. 1999
    ..These results indicate that human sPLA(2)-X is a unique N-glycosylated sPLA(2) that releases arachidonic acid from human myeloid leukemia cells more efficiently than sPLA(2)-IB and -IIA...
  74. ncbi Ca2+-independent phospholipase A2 is required for agonist-induced Ca2+ sensitization of contraction in vascular smooth muscle
    Zhenheng Guo
    Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    J Biol Chem 278:1856-63. 2003
    ..Our data provide the first evidence that iPLA(2) is required for maintaining basal free arachidonic acid levels and thus is essential for agonist-induced Ca(2+)-sensitization of contraction in vascular smooth muscle...
  75. ncbi Systematic evaluation of transcellular activities of secretory phospholipases A2. High activity of group V phospholipases A2 to induce eicosanoid biosynthesis in neighboring inflammatory cells
    Gihani T Wijewickrama
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 281:10935-44. 2006
    ....
  76. ncbi Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2
    J D Sharp
    Lilly Research Laboratories, Indianapolis, Indiana 46285
    J Biol Chem 266:14850-3. 1991
    ..The molecular cloning of cPLA2 will allow further studies defining the structure, function, and regulation of this novel PLA2...
  77. ncbi Cytosolic phospholipase A2 (cPLA2) regulation of human monocyte NADPH oxidase activity. cPLA2 affects translocation but not phosphorylation of p67(phox) and p47(phox)
    Xiaoxian Zhao
    Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Ohio 44195, USA
    J Biol Chem 277:25385-92. 2002
    ..These studies provide insight into the mechanisms by which activation signals are transduced to allow the induction of superoxide anion production in human monocytes...
  78. pmc The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis
    S Ishiguro
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Plant Cell 13:2191-209. 2001
    ..DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals...
  79. ncbi Patatin-related phospholipase A: nomenclature, subfamilies and functions in plants
    Günther F E Scherer
    Leibniz Universitat Hannover, Inst f Zierpflanzenbau and Gehölzforschung, Abt Molekulare Ertragsphysiologie, Herrenhauser Str 2, D 30419 Hannover, Germany
    Trends Plant Sci 15:693-700. 2010
    ..In this review, we highlight recent developments regarding these enzymes and propose the nomenclature pPLA for the patatin-related phospholipase A enzyme...
  80. ncbi Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency
    Steffen Rietz
    Max Planck Institute for Plant Breeding Research, Department of Plant Microbe Interactions, Carl von Linne Weg 10, D 50829 Cologne, Germany
    Mol Plant 3:524-38. 2010
    ..Taken together, the data reveal novel functions of pPLAs in root development with individual roles at the interface between phosphate deficiency and auxin signaling...
  81. pmc PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis
    Lingfeng Chen
    Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 12:603-14. 2007
    ..We propose that PLA(2) and PI3K signaling act in concert to mediate chemotaxis, and metabolites of PLA(2) may be important mediators of the response...
  82. ncbi Multiple forms of secretory phospholipase A2 in plants
    Hyoung Yool Lee
    Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790 784, Korea
    Prog Lipid Res 44:52-67. 2005
    ..This review discusses the newly-acquired information on plant sPLA2s including the molecular and biochemical characteristics, and signaling functions of each isoform...
  83. pmc An essential role of cytosolic phospholipase A2alpha in prostaglandin E2-mediated bone resorption associated with inflammation
    Chisato Miyaura
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Exp Med 197:1303-10. 2003
    ..Here, we show that cPLA2alpha plays a key role in PGE production by osteoblasts and in osteoclastic bone resorption, and suggest a new approach to inflammatory bone disease by inhibiting cPLA2alpha...
  84. ncbi Identification of novel cytosolic phospholipase A(2)s, murine cPLA(2){delta}, {epsilon}, and {zeta}, which form a gene cluster with cPLA(2){beta}
    Takayo Ohto
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 280:24576-83. 2005
    ..We thus discovered these functional novel cPLA(2) genes, which cluster on murine chromosome 2E5...
  85. ncbi Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2
    Alan G Singer
    Department of Chemistry, University of Washington, Seattle 98195, USA
    J Biol Chem 277:48535-49. 2002
    Expression of the full set of human and mouse groups I, II, V, X, and XII secreted phospholipases A(2) (sPLA(2)s) in Escherichia coli and insect cells has provided pure recombinant enzymes for detailed comparative interfacial kinetic and ..
  86. ncbi Suppression of intestinal polyposis in Apc(delta 716) knockout mice by an additional mutation in the cytosolic phospholipase A(2) gene
    K Takaku
    Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Biol Chem 275:34013-6. 2000
    ..These results suggest that in the colon, group X sPLA(2) supplies arachidonic acid in both the normal epithelium and the polyps even in the absence of cPLA(2)...
  87. ncbi Phospholipid-derived signaling mediated by phospholipase A in plants
    Stephen B Ryu
    Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790 784, Korea
    Trends Plant Sci 9:229-35. 2004
  88. ncbi Phospholipid signalling in plant defence
    Ana M Laxalt
    Swammerdam Institute for Life Sciences, Department of Plant Physiology, University of Amsterdam, Kruislaan 318, NL 1098 SM, Amsterdam, The Netherlands
    Curr Opin Plant Biol 5:332-8. 2002
    ....
  89. ncbi Structure, function and regulation of Ca2+-sensitive cytosolic phospholipase A2 (cPLA2)
    R M Kramer
    Cardiovascular Research, Lilly Research Laboratories, Indianapolis, IN 46285, USA
    FEBS Lett 410:49-53. 1997
    ..These products of cPLA2 contribute to inflammatory and degenerative disease states and cPLA2 is therefore an attractive target for the development of novel therapies...
  90. ncbi Acute lung injury by sepsis and acid aspiration: a key role for cytosolic phospholipase A2
    T Nagase
    Department of Geriatric Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113, Japan
    Nat Immunol 1:42-6. 2000
    ..Thus, the inhibition of cPLA2-initiated pathways may provide a therapeutic approach to acute lung injury, for which no pharmaceutical agents are currently effective...
  91. ncbi Intracellular calcium signals regulating cytosolic phospholipase A2 translocation to internal membranes
    J H Evans
    Program in Cell Biology, Department of Pediatrics, and Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 276:30150-60. 2001
    ..These results suggest that the cPLA(2) C2 domain regulates differential, Ca(2+)-dependent membrane targeting and that the catalytic domain regulates both the rate of translocation and enzyme residence...
  92. ncbi Molecular cloning of phospholipases A(2) from venom glands of Echis carpet vipers
    K Bharati
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA, Liverpool, UK
    Toxicon 41:941-7. 2003
    ..However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA(2)-neutralizing antibodies with polyspecific potential...
  93. ncbi Possible involvement of Ca2+-independent phospholipase A2 in protease-activated receptor-2-mediated contraction of rat urinary bladder
    Yuko Kubota
    Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5 9 1 Shirokane, 108 8641 Minato ku, Tokyo, Japan
    Naunyn Schmiedebergs Arch Pharmacol 367:588-91. 2003
    ..The release of prostaglandins by PAR-2 activators seems to be partly mediated by the iPLA2...
  94. ncbi Accelerated evolution and molecular surface of venom phospholipase A2 enzymes
    R M Kini
    Bioscience Centre, Faculty of Science, National University of Singapore, 10, Kent Ridge Crescent, Singapore 119260
    J Mol Evol 48:125-32. 1999
    ..Thus natural substitutions in PLA2 enzymes, in contrast to popular belief, are not random substitutions but appear to be directed toward modifying the molecular surface...
  95. ncbi Calcium-independent phospholipase A(2) is required for human monocyte chemotaxis to monocyte chemoattractant protein 1
    K A Carnevale
    Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 167:3414-21. 2001
    ..These data demonstrate novel and distinct roles for these two phospholipases in this critical step in inflammation...
  96. ncbi Molecular cloning of two new human paralogs of 85-kDa cytosolic phospholipase A2
    R T Pickard
    Lilly Research Laboratory, Indianapolis, Indiana 46285, USA
    J Biol Chem 274:8823-31. 1999
    ..Mutagenesis shows strong dependence on these residues for catalytic activity of all three enzymes...
  97. ncbi Secretory phospholipase A2 from Arabidopsis thaliana: insights into the three-dimensional structure and the amino acids involved in catalysis
    Johanna Mansfeld
    Institute of Biotechnology, Department of Biochemistry Biotechnology, Martin Luther University Halle Wittenberg, D 06120 Halle, Germany
    Biochemistry 45:5687-94. 2006
    ..Mutagenesis of amino acids supposed to be involved in catalysis has shown that His62, the calcium-coordinating Asp63, and the above-mentioned Ser79 residue are essential for activity...
  98. ncbi Characterization of Arabidopsis secretory phospholipase A2-gamma cDNA and its enzymatic properties
    Sung Chul Bahn
    School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea
    FEBS Lett 553:113-8. 2003
    Plant secretory phospholipases A(2) (sPLA(2)s) probably play important roles in phospholipid signaling based on the data reported from other organisms, but their functions are poorly understood because of the lack of cloned sPLA(2) genes...
  99. ncbi Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism
    A Dessen
    Biochemistry, Wyeth Research, Cambridge, Massachusetts 02140, USA
    Cell 97:349-60. 1999
    ..The structure reveals a flexible lid that must move to allow substrate access to the active site, thus explaining the interfacial activation of this important lipase...
  100. ncbi The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2
    Robert V Stahelin
    Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    J Biol Chem 278:12452-60. 2003
    ..Together, these results suggest that biophysical principles that govern the in vitro membrane binding of C2 domains can account for most of their subcellular targeting properties...
  101. ncbi cAMP elevating agents suppress secretory phospholipase A(2)-induced matrix metalloproteinase-2 activation
    ChuHee Lee
    Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu 705 035, South Korea Aging Associated Vascular Disease Research Center, Daegu 705 035, South Korea
    Biochem Biophys Res Commun 340:1278-83. 2006
    ..Taken together, our data indicate that sPLA2-induced proMMP-2 activation is influenced by intracellular cAMP levels via the modulations of TIMP-2 expression and ERK1/2 activation...

Research Grants63

  1. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Alex Brown; Fiscal Year: 2010
    ..b>Phospholipases A generate fatty acids and receptor-active lysophospholipids...
  2. 48th Annual South Eastern Regional Lipid Conference (SERLC) Funding Support
    Carole A Oskeritzian; Fiscal Year: 2013
    ..For example, phospholipases A generate fatty acids (FA) and lysophospholipids...
  3. LUNG INFLAMMATION
    Gerald Weissmann; Fiscal Year: 1999
    ..To test the hypothesis that the physical state of phospholipids (lamellar vs Hex(II)) as regulated by phospholipases A, C and D regulates a) the association of prenylated proteins with the plasmalemma and b) the effect of ..
  4. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Andrew Morris; Fiscal Year: 2004
    ..b>Phospholipases A generate fatty acids and lysophospholipids...
  5. South Eastern Region Lipid Conference 2006
    Charles Chalfant; Fiscal Year: 2007
    ..For example, [unreadable] phospholipases A generate fatty acids and lysophospholipids...
  6. CDP-choline: Mechanisms in Cerebral Ischemia
    RAO ADIBHATLA; Fiscal Year: 2006
    ..Arachidonic acid activates neutral sphingomyelinase, resulting in membrane disintegration. PCCT activity was stimulated by exogenous CDP-choline. Aim 2 will be tested in gerbil transient forebrain ischemia. ..
  7. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  8. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  9. BIOCHEMICAL STUDIES OF 14 KDA PHOSPHOLIPASES A2
    Michael Gelb; Fiscal Year: 2006
    ..These studies will contribute to our fundamental understanding of how mammalian cells initiate the eicosanoid cascade. ..
  10. METABOLISM OF PLATELET-ACTIVATING FACTOR
    Diana Stafforini; Fiscal Year: 2008
    ..III. Determine if deletion of PAF-AH alters the severity of necrotizing enterocolitis using animal models. IV. Test the hypothesis that PAF-AH alters atherosclerosis susceptibility using genetically engineered models. ..
  11. MULTIPLEX ANALYSIS OF INBORN ERRORS OF METABOLISM
    Michael Gelb; Fiscal Year: 2001
    ..abstract_text> ..
  12. Conference on Drug Against Tropical Protozoan Parasites
    Michael Gelb; Fiscal Year: 2002
    ..We consider the poster sessions that occur throughout our meeting to be just as important as the collection of lecture presentations. ..
  13. BIOCHEMISTRY OF PROTEIN PRENYLATION
    Michael Gelb; Fiscal Year: 2002
    ....
  14. MAC 1/LFA 1/P150 95 AND PMN LEUKOCYTE FUNCTION
    Christie Ballantyne; Fiscal Year: 2002
    ..3. Characterize the contribution of each CD11 integrin on the host response to common bacterial and fungal pathogens in vitro and in vivo. ..
  15. Obesity, Inflammation and Thrombosis: LOOK AHEAD
    Christie M Ballantyne; Fiscal Year: 2010
    ....
  16. The Role of PAF and TLR in NEC
    Michael S Caplan; Fiscal Year: 2010
    ..The proposed studies will elucidate the interactions between PAF and TLR signaling, and clarify whether these pathways play an underlying role in the initiation of neonatal NEC. ..
  17. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
    ..abstract_text> ..
  18. Chemogenomics to Identify New Molecular Targets in PF
    William Henderson; Fiscal Year: 2006
    ..These studies represent a focused effort using chemogenomics to identify, develop, and validate small molecule inhibitors of AP-1/TGFBeta-driven profibrotic gene expression as novel therapies in PF patients. ..
  19. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis Scott; Fiscal Year: 2009
    ..We hypothesize that PG and phospholipases stimulate HM dendricity, MS transfer &pigmentation through different mechanisms that act synergistically to modulate cutaneous pigmentation following UVR. ..
  20. 5-LIPOXYGENASE PRODUCTS IN ASTHMATIC IMMUNE RESPONSE
    William Henderson; Fiscal Year: 2008
    ..abstract_text> ..
  21. Studies of Diacylglycerol-Binding Proteins
    Wonhwa Cho; Fiscal Year: 2009
    ....
  22. Development of Hydrogel Spinal Disc Replacement
    Barbara Boyan; Fiscal Year: 2004
    ..abstract_text> ..
  23. INTERFACIAL CATALYSIS BY PHOSPHOLIPASE A2
    Mahendra Jain; Fiscal Year: 2004
    ..These results will provide insights into the structural basis for the functional differences between the coupling of the i-face with the active site events of the PLA2 family. ..
  24. Structure Function Studies Of Membrane Binding Proteins
    Wonhwa Cho; Fiscal Year: 2004
    ....
  25. 1-Cys-peroxiredoxin, A Novel Lung Anti-oxidant Enzyme
    Aron Fisher; Fiscal Year: 2004
    ..These studies will give insights into the function and antioxidant activity of this potentially important, newly described enzyme. ..
  26. Conference on Oxidative Mechanisms in Neurodegeneration
    Grace Sun; Fiscal Year: 2004
    ..In addition, plans are in progress to provide rapid publication of the symposium proceedings in an internationally recognized neuroscience journal. ..
  27. SICKLE RBC, INTERMITTENT HYPOXIA AND VASCULAR PATHOLOGY
    FRANS KUYPERS; Fiscal Year: 2003
    ..Together, our results may indicate novel treatment regiments in the management of SCD. ..
  28. Regulatory Mechanisms of Secretory Phospholipases A2
    SUREN TATULIAN; Fiscal Year: 2004
    ..Collectively, our results will help develop new strategies for regulation of PLA2s, enzymes of profound biomedical importance. ..
  29. Influence of Sex and Gender on Musculoskeletal Health
    Barbara Boyan; Fiscal Year: 2004
    ..Worldwide current and future clinical trials will be discussed. Short papers on the workshop topics, future directions, and scientific updates will be published via Internet and summary papers in peer-reviewed journals. ..
  30. ROLE OF MEMBRANES AND MINERALIZATION OF BONES AND TEETH
    Barbara Boyan; Fiscal Year: 2001
    ..This work will have major implications for our understanding of MV and how cells that calcify their matrix are able to regulate these extracellular organelles at distant sites. ..
  31. BONE FACTORS AND ENDOCHONDRAL OSSIFICATION
    Barbara Boyan; Fiscal Year: 2001
    ..It is suggested that these results will provide insight into the role these factors play in bone development and repair. ..
  32. Chlamydia Infection as a Risk Factor for Cerebrovascula*
    Lori Styles; Fiscal Year: 2004
    ..The data gained in this proposal would provide the preliminary data necessary to justify further clinical trials. ..
  33. Acyl-CoAs and Lesion Initiation in Diabetes
    KARIN BORNFELDT; Fiscal Year: 2008
    ..Identification of such mechanisms might be used to develop drugs to target cardiovascular complications of type 1 diabetes. [unreadable] [unreadable] [unreadable]..
  34. Crosslinkable Hydrogels for Tympanic Membrane Repair
    Glenn Prestwich; Fiscal Year: 2004
    ..Third, we will evaluate the biocompability and otocompability of each gel in vivo by evaluating recovery of auditory function and middle ear histology. ..
  35. Small molecule inhibitors of bacterial secretion system
    Vincent Lee; Fiscal Year: 2008
    ..abstract_text> ..
  36. LONG-CHAIN ACYL-COA SYNTHETASES IN VASCULAR CELLS
    KARIN BORNFELDT; Fiscal Year: 2007
    ..The ACS isoforms in vascular cells have not been studied to date. We expect to identify ACS isoforms required for important biological effects of OA in primary smooth muscle and macrophages. [unreadable] [unreadable]..
  37. ENDOTHELIAL SIGNALING MECHANISMS IN LUNG INJURY
    Sunita Bhattacharya; Fiscal Year: 2005
    ..These mechanisms are highly relevant to the general understanding of the lung inflammatory response. No previous understanding of these mechanisms exists for lung endothelium in vivo. The proposed studies will be novel. ..
  38. Pain Mechanisms and the Development of Analgesics
    Tony Yaksh; Fiscal Year: 2006
    ..g. COX2 inhibitors, GABApentin, ziconotide) and failures (NK1 antagonist). [unreadable] [unreadable] [unreadable]..
  39. HDL metabolism: Influence of extracellular lipases
    Daniel Rader; Fiscal Year: 2009
    ....
  40. Mechanisms of Renal Na+ Pump Stimulation by Angiotensin
    DOUGLAS YINGST; Fiscal Year: 2005
    ..These results have important implications for understanding normal kidney function, as well as the development of hypertension and heart failure. ..
  41. An interdisciplinary and integrative study of tendinopathy
    James H C Wang; Fiscal Year: 2010
    ....
  42. Thiol-Reactive Crosslinkers for Biomaterials
    Glenn Prestwich; Fiscal Year: 2004
    ..Fourth, these new materials will be evaluated for cytocompatibility and cell growth in vitro with murine fibroblasts. Finally, selected materials will be seeded with fibroblasts and injected into nude mice for in vivo tissue generation. ..
  43. Folding and design of beta sheets in membranes
    William C Wimley; Fiscal Year: 2010
    ..This information will be used to create a public database of potential outer membrane proteins ..
  44. Lipid Maps
    EDWARD DENNIS; Fiscal Year: 2002
    ..This functional characterization, coupled with our knowledge of other macromolecules and cell processes, will enable the community as a whole to establish predictive models for homeostasis and drug responses in disease states. ..
  45. MOLECULAR BIOLOGY AND FUNCTIONS OF EOSINOPHIL PROTEINS
    Steven Ackerman; Fiscal Year: 2005
    ....
  46. CELLULAR AND MOLECULAR RESPONSES IN LUNG DISEASE
    Guy Zimmerman; Fiscal Year: 2003
    ..abstract_text> ..
  47. STROKE RISK IN CHILDREN WITH SICKLE CELL ANEMIA
    Lori Styles; Fiscal Year: 2002
    ..Development of a new PCR-based multiplex assay strip specifically for use in assessing stroke risk in children with SCA. ..
  48. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani Subbaiah; Fiscal Year: 2008
    ..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly open up novel therapeutic targets against inflammation and atherosclerosis. ..
  49. CELLULAR REGULATION OF PROSTAGLANDIN SYNTHESIS
    Andrey Sorokin; Fiscal Year: 2009
    ....
  50. MECHANISM OF PLATELET ACTIVATION ON MATRIX PROTEINS
    Beatrice Haimovich; Fiscal Year: 2004
    ..phosphatase(s) and kinase(s) regulate the state of phosphorylation of a-actinin? Aim 3: What is the role of tyrosine phosphorylation of pplOl and vinculin, and which phosphatase(s) and kinase(s) regulate these phosphorylation events? ..
  51. PAIN EVOKED RELEASE OF AMINO ACIDS AND PROSTANOIDS
    Tony Yaksh; Fiscal Year: 2005
    ....
  52. MECHANISM OF PI-RELATED DYSLIPIDEMIA AND ATHEROSCLEROSIS
    Daniel Rader; Fiscal Year: 2004
    ..These studies will provide novel insights into the mechanisms of PI-associated dyslipidemia and atherosclerosis. ..
  53. Spinal Galanin and its Receptors in Pain Processing
    Tony Yaksh; Fiscal Year: 2005
    ..We believe the outcome of these studies will provide direct evidence for the role played by this spinal peptidergic system in pain and point to the development of novel anti-hyperpathic agents. ..
  54. Transcription Regulation and Biological Function of NAG1
    SEUNG BAEK; Fiscal Year: 2005
    ..Cancer and inflammation are certainly environmentally relevant problems and thus this project will provide new clues to aid in the understanding of how these drugs alter tumor development. ..
  55. Molecular Aspects of Myeloid Development and Leukemia
    Steven Ackerman; Fiscal Year: 2005
    ..abstract_text> ..
  56. Anti-cancer effects by the green tea catechins
    SEUNG BAEK; Fiscal Year: 2006
    ..2. Profile changes in gene expression following ECG treatment. 3. Compare the preventative and therapeutic efficacy of individual and combination catechins in animal models of colorectal cancer. ..
  57. Host genes required for P. aeruginosa pathogenesis
    Vincent Lee; Fiscal Year: 2004
    ..abstract_text> ..
  58. Urothelial Cell Physiology in Normal and Disease States
    Ricardo Saban; Fiscal Year: 2005
    ..Scott-Hultgren (Washington U) will discuss the molecular basis of persistent bladder infections. The bladder cancer discussion will be chaired by Drs. Robert Hurst (Oklahoma U) and Jenny Southgate (U. York). ..
  59. R21: Hypocholesterolemic action of green tea extracts
    Sung Koo; Fiscal Year: 2005
    ..This study also will yield valuable information on whether green tea and catechins can be recommended as a means of lowering cholesterol absorption, and will form the basis for further mechanistic or clinical investigations. ..
  60. LPS-PEPTIDE INTERACTION IN BLADDER INFLAMMATION
    Ricardo Saban; Fiscal Year: 2007
    ..Information generated by this study should elucidate whether bladder inflammatory responses, regardless of etiology, occur via a common mechanism or whether the endpoints are specific for each stimulus. ..
  61. Phospholipases in the Pathogenesis of M. tuberculosis
    Sarah Parker; Fiscal Year: 2006
    ..Dr. Parker is committed to research in M. tuberculosis, and it holds a special significance to her because of her life experiences. ..