carboxylic ester hydrolases

Summary

Summary: Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.

Top Publications

  1. ncbi Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase
    Y H Dong
    Institute of Molecular Agrobiology, National University of Singapore, Singapore
    Nature 411:813-7. 2001
  2. ncbi New insights into pectin methylesterase structure and function
    Jérôme Pelloux
    Groupe de Génomique Fonctionnelle des Plantes, EA 3900, Faculte des Sciences, Universite de Picardie Jules Verne, 33 rue St Leu, 80039 Amiens, France
    Trends Plant Sci 12:267-77. 2007
  3. pmc The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase
    Myung Hee Kim
    Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305 600, Korea
    Proc Natl Acad Sci U S A 102:17606-11. 2005
  4. ncbi Pectin methylesterases: cell wall enzymes with important roles in plant physiology
    F Micheli
    Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, INRA CNRS, BP27, 31326 Castanet Tolosan Cedex, France
    Trends Plant Sci 6:414-9. 2001
  5. pmc A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome
    Ricarda Richter
    Mitochondrial Research Group, Institute for Ageing and Health, Medical School, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 29:1116-25. 2010
  6. ncbi Pectin-induced changes in cell wall mechanics underlie organ initiation in Arabidopsis
    Alexis Peaucelle
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, 78026 Versailles, France
    Curr Biol 21:1720-6. 2011
  7. ncbi Structural basis for natural lactonase and promiscuous phosphotriesterase activities
    Mikael Elias
    Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS Université Henri Poincaré, 54506 Nancy, France
    J Mol Biol 379:1017-28. 2008
  8. pmc Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species
    Yi Hu Dong
    Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604
    Appl Environ Microbiol 68:1754-9. 2002
  9. pmc Structural basis for the interaction between pectin methylesterase and a specific inhibitor protein
    Adele Di Matteo
    Department of Biochemical Sciences, University of Rome, 00185 Rome, Italy
    Plant Cell 17:849-58. 2005
  10. ncbi Microbial carboxyl esterases: classification, properties and application in biocatalysis
    Uwe T Bornscheuer
    Institute for Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr 16, Greifswald, Germany
    FEMS Microbiol Rev 26:73-81. 2002

Detail Information

Publications289 found, 100 shown here

  1. ncbi Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase
    Y H Dong
    Institute of Molecular Agrobiology, National University of Singapore, Singapore
    Nature 411:813-7. 2001
    ..carotovora infection. Our results highlight a promising potential to use quorum-sensing signals as molecular targets for disease control, thereby broadening current approaches for prevention of bacterial infections...
  2. ncbi New insights into pectin methylesterase structure and function
    Jérôme Pelloux
    Groupe de Génomique Fonctionnelle des Plantes, EA 3900, Faculte des Sciences, Universite de Picardie Jules Verne, 33 rue St Leu, 80039 Amiens, France
    Trends Plant Sci 12:267-77. 2007
    ..Although, overall, protein structures are highly conserved between isoforms, recent data indicate that structural variations might be associated with the targeting and functions of specific pectin methylesterases...
  3. pmc The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase
    Myung Hee Kim
    Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305 600, Korea
    Proc Natl Acad Sci U S A 102:17606-11. 2005
    ..We present experimental evidence that AHL-lactonase is a metalloenzyme containing two zinc ions involved in catalysis, and we propose a catalytic mechanism for bacterial metallo-AHL-lactonases...
  4. ncbi Pectin methylesterases: cell wall enzymes with important roles in plant physiology
    F Micheli
    Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, INRA CNRS, BP27, 31326 Castanet Tolosan Cedex, France
    Trends Plant Sci 6:414-9. 2001
    ....
  5. pmc A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome
    Ricarda Richter
    Mitochondrial Research Group, Institute for Ageing and Health, Medical School, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 29:1116-25. 2010
    ..We suggest that ICT1 may be essential for hydrolysis of prematurely terminated peptidyl-tRNA moieties in stalled mitoribosomes...
  6. ncbi Pectin-induced changes in cell wall mechanics underlie organ initiation in Arabidopsis
    Alexis Peaucelle
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, 78026 Versailles, France
    Curr Biol 21:1720-6. 2011
    ....
  7. ncbi Structural basis for natural lactonase and promiscuous phosphotriesterase activities
    Mikael Elias
    Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS Université Henri Poincaré, 54506 Nancy, France
    J Mol Biol 379:1017-28. 2008
    ..This example demonstrates that promiscuous activities probably constitute a large and efficient reservoir for the creation of novel catalytic activities...
  8. pmc Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species
    Yi Hu Dong
    Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604
    Appl Environ Microbiol 68:1754-9. 2002
    ..Sequence comparison of the gene products and motif analysis showed that the gene products belong to the same family of AHL lactonases...
  9. pmc Structural basis for the interaction between pectin methylesterase and a specific inhibitor protein
    Adele Di Matteo
    Department of Biochemical Sciences, University of Rome, 00185 Rome, Italy
    Plant Cell 17:849-58. 2005
    ..The structure of the complex gives an insight into the specificity of the inhibitor toward plant PMEs and the mechanism of regulation of these enzymes...
  10. ncbi Microbial carboxyl esterases: classification, properties and application in biocatalysis
    Uwe T Bornscheuer
    Institute for Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr 16, Greifswald, Germany
    FEMS Microbiol Rev 26:73-81. 2002
    ..Special emphasis is given on their application in organic synthesis for the resolution of racemates and prostereogenic compounds. In addition, recent results for altering their properties by directed evolution are presented...
  11. ncbi Structure and specificity of a quorum-quenching lactonase (AiiB) from Agrobacterium tumefaciens
    Dali Liu
    Department of Chemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Biochemistry 46:11789-99. 2007
    ..This work should help elucidate the hydrolytic ring-opening mechanism of this family of enzymes and also facilitate the design of more effective quorum-quenching catalysts...
  12. ncbi A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls
    Sandra Pelletier
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, INRA Centre de Versailles Grignon, Versailles, France
    New Phytol 188:726-39. 2010
    ..This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event...
  13. pmc Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4
    B J Feys
    The Sainsbury Laboratory, John Innes Centre, Colney, Norwich NR4 7UH, UK
    EMBO J 20:5400-11. 2001
    ..We propose two functions for EDS1. The first is required early in plant defence, independently of PAD4. The second recruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association...
  14. ncbi Pectin methylesterase and its proteinaceous inhibitor: a review
    Ruben P Jolie
    Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre LFoRCe, Department of Microbial and Molecular Systems M2S, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, PB 2457, B 3001 Leuven, Belgium
    Carbohydr Res 345:2583-95. 2010
    ..Remaining research challenges are indicated...
  15. pmc Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea
    Vincenzo Lionetti
    Dipartimento di Biologia Vegetale, Universita di Roma La Sapienza, 00185 Rome, Italy
    Plant Physiol 143:1871-80. 2007
    ..The reduced symptoms caused by the fungus on transgenic plants were related to its impaired ability to grow on methylesterified pectins...
  16. pmc Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity
    Bart J Feys
    Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 17:2601-13. 2005
    ..These data, combined with evidence for coregulation between individual EDS1 complexes, suggest that dynamic interactions of EDS1 and its signaling partners in multiple cell compartments are important for plant defense signal relay...
  17. pmc Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens
    Hai Bao Zhang
    Laboratory of Biosignals and Bioengineering, Institute of Molecular Agrobiology, National University of Singapore, 1 Research Link, Singapore 117604
    Proc Natl Acad Sci U S A 99:4638-43. 2002
    ..These data suggest that A. tumefaciens has a sophisticated multicomponent quorum-sensing signal turnover system, allowing the cell to sense a change in growth and adjust cellular activities accordingly...
  18. ncbi Plant protein inhibitors of cell wall degrading enzymes
    Nathalie Juge
    Institut Méditerranéen de Recherche en Nutrition, Faculté des Sciences de St Jérôme, F 13397 Marseilles Cedex 20, France
    Trends Plant Sci 11:359-67. 2006
    ....
  19. ncbi Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunity
    Steffen Rietz
    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Koln, Germany
    New Phytol 191:107-19. 2011
    ..Different EDS1 and PAD4 molecular configurations have distinct and separable functions in the plant innate immune response...
  20. ncbi Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis
    Josep A Villena
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    J Biol Chem 279:47066-75. 2004
    ..In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity...
  21. pmc Orally administered thermostable N-acyl homoserine lactonase from Bacillus sp. strain AI96 attenuates Aeromonas hydrophila infection in zebrafish
    Yanan Cao
    Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People s Republic of China
    Appl Environ Microbiol 78:1899-908. 2012
    ..This is the first report of the oral administration of an AHL lactonase for the efficient control of A. hydrophila...
  22. ncbi Caterpillar-elicited methanol emission: a new signal in plant-herbivore interactions?
    Caroline C von Dahl
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, Hans Knoll Strasse 8, 07745 Jena, Germany
    Plant J 46:948-60. 2006
    ....
  23. ncbi A novel function for a ubiquitous plant enzyme pectin methylesterase: the enhancer of RNA silencing
    Yuri L Dorokhov
    Department of Virology and A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Leninsky Gory 1, Laboratory Building A, Moscow 119992, Russia
    FEBS Lett 580:3872-8. 2006
    ..It was hypothesized that DCL1 relocated to the cytoplasm may use as substrates both miRNA precursor and viral RNA. The capacity for enhancing the RNA silencing is a novel function for the polyfunctional PME...
  24. ncbi The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatus
    Sebastian Wolf
    Heidelberg Institute for Plant Sciences, INF 360, 69120 Heidelberg, Germany
    Plant J 58:361-75. 2009
    ..Consequently, pro-protein processing could constitute a post-translational mechanism regulating PME activity...
  25. pmc AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum
    Wen Zhao Wang
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7 1 2 Yoto, Utsunomiya 321 8585, Japan
    Appl Environ Microbiol 76:2524-30. 2010
    ..carotovorum reduced pectinase activity markedly and attenuated soft rot symptoms on potato slices. In conclusion, this study indicated that AiiM might be effective in quenching quorum sensing of P. carotovorum subsp. carotovorum...
  26. pmc The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity
    A Carlier
    Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Appl Environ Microbiol 69:4989-93. 2003
    ..In Erwinia strain 6276, the lactonases reduced the endogenous acyl-HSL level and the bacterial virulence in planta...
  27. pmc AidH, an alpha/beta-hydrolase fold family member from an Ochrobactrum sp. strain, is a novel N-acylhomoserine lactonase
    Gui Ying Mei
    Department of Plant Pathology, China Agricultural University, Beijing 100193, China
    Appl Environ Microbiol 76:4933-42. 2010
    ....
  28. pmc Pectin methylesterases and pectin dynamics in pollen tubes
    Maurice Bosch
    School of Biosciences, University of Birmingham, UK
    Plant Cell 17:3219-26. 2005
  29. ncbi AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria
    Sun Yang Park
    R and D Center, inBioNET Corporation, Daejeon 305 390, Korea
    Microbiology 149:1541-50. 2003
    ..Furthermore, it was verified that ahlK, an ahlD homologue, encodes an AHL-degrading enzyme in K. pneumoniae. Accordingly, the current results suggest the possibility that AhlD-like AHLases could exist in many other micro-organisms...
  30. ncbi Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants
    M Aviram
    The Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel
    Free Radic Biol Med 26:892-904. 1999
    ..e. prevention of its formation, and removal of Ox-LDL associated oxidized lipids which are generated during LDL oxidation...
  31. pmc YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes
    Yoshihiro Handa
    Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 1 5 1 Tenjin cho, Kiryu shi, Gunma 376 8515, Japan
    Nucleic Acids Res 39:1739-48. 2011
    ..The C-terminal basic residue-rich extension was also found to be required for ribosome binding. These findings suggest that YaeJ functions as a ribosome-attached rescue device for stalled ribosomes...
  32. ncbi Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways
    Yuhei Chadani
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Mol Microbiol 80:772-85. 2011
    ..It was also suggested that ArfA and YaeJ rescue the stalled ribosomes by distinct mechanisms...
  33. ncbi Plant cell walls: Protecting the barrier from degradation by microbial enzymes
    Stijn Lagaert
    Division of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, BE 3001 Leuven, Belgium
    Semin Cell Dev Biol 20:1064-73. 2009
    ....
  34. pmc Metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in Pseudomonas aeruginosa
    C Schipper
    Biozentrum Klein Flottbek Mikrobiologie, University of Hamburg, Ohnhorststr 18, D 22609 Hamburg, Germany
    Appl Environ Microbiol 75:224-33. 2009
    ..aeruginosa when expressed in P. aeruginosa PAO1. Thus, this is the first study in which metagenome-derived proteins have been expressed in P. aeruginosa to successfully inhibit biofilm formation...
  35. pmc Thermostable variants of cocaine esterase for long-time protection against cocaine toxicity
    Daquan Gao
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40536, USA
    Mol Pharmacol 75:318-23. 2009
    ..The novel design strategy can be used to develop thermostable mutants of any protein...
  36. ncbi Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors
    A Raiola
    Dipartimento di Biologia Vegetale, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Roma, Italy
    FEBS Lett 557:199-203. 2004
    ..The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species...
  37. pmc Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast
    Guillermina Rosas-Sandoval
    Departments of Molecular Biophysics and Biochemistry and Chemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 99:16707-12. 2002
    ..Deletions in Saccharomyces cerevisiae of the pth2 (YBL057c) or pth (YHR189w) orthologs were viable, as was the double deletion strain, implying that the canonical Pth and Pth2 enzymes are not essential for yeast viability...
  38. pmc Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism, signal interference
    Yi Hu Dong
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore 117609
    Appl Environ Microbiol 70:954-60. 2004
    ..These results suggest that signal interference mechanisms existing in natural ecosystems could be explored as a new version of antagonism for prevention of bacterial infections...
  39. ncbi Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima
    Mark Levisson
    Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 GT Wageningen, The Netherlands
    FEBS J 274:2832-42. 2007
    ..A comparison of the active site pentapeptide motifs revealed that EstD should be grouped into a new family of esterases (Family 10). EstD is the first characterized member of this family...
  40. ncbi Pectin methylesterase inhibitor
    A Giovane
    Department of Biochemistry and Biophysics, 2nd University of Napoli, Via Costantinopoli 16, I 80138, Naples, Italy
    Biochim Biophys Acta 1696:245-52. 2004
    ..Affinity chromatography on resin-bound PMEI can also be used to concentrate and detect residual PME activity in fruit and vegetable products...
  41. ncbi Distinction between esterases and lipases: a kinetic study with vinyl esters and TAG
    Henri Chahinian
    Laboratoire de Lipolyse Enzymatique, CNRS, Marseille, France
    Lipids 37:653-62. 2002
    ..In this respect, it clearly appears that guinea pig pancreatic lipase and cutinase are unambiguously classified as lipases...
  42. ncbi Characterization of enzymes involved in formation of ethyl esters of long-chain fatty acids in humans
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    J Lipid Res 42:1025-32. 2001
    ..A very low rate of FAEE hydrolysis was detected in heart and adipose tissue, which probably accounts for the previously observed accumulation of FAEE in these organs...
  43. pmc Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase
    Kosuke Ito
    Department of Biology, Faculty of Science, Niigata University, 8050 Ikarashi 2 no cho, Nishi ku, Niigata 950 2181, Japan
    Nucleic Acids Res 40:10521-31. 2012
    ..Furthermore, we propose an authentic Pth:peptidyl-tRNA complex model and a detailed mechanism for the hydrolysis reaction, based on the present crystal structure and the previous studies' results...
  44. pmc Radial distribution pattern of pectin methylesterases across the cambial region of hybrid aspen at activity and dormancy
    F Micheli
    Laboratoire d Enzymologie en Milieu Structuré, Département de Biologie Supramoléculaire et Cellulaire, Institut Jacques Monod, 2 place Jussieu, F 75252 Paris Cedex 05, France
    Plant Physiol 124:191-9. 2000
    ....
  45. ncbi Deficiency of carboxylesterase 1/esterase-x results in obesity, hepatic steatosis, and hyperlipidemia
    Ariel D Quiroga
    Department of Pediatrics, University of Alberta, Edmonton, Canada
    Hepatology 56:2188-98. 2012
    ....
  46. ncbi Role of pectin methylesterases in cellular calcium distribution and blossom-end rot development in tomato fruit
    Sergio T de Freitas
    Department of Plant Sciences, University of California, Davis, CA 95616, USA
    Plant J 71:824-35. 2012
    ..Our results suggest that apoplastic water-soluble Ca²⁺ concentration influences fruit susceptibility to Ca²⁺ deficiency disorders...
  47. ncbi Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris
    Tomohiro Morohoshi
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7 1 2 Yoto, Utsunomiya 321 8585, Japan
    J Biosci Bioeng 113:20-5. 2012
    ..carotovorum attenuated maceration of the potato slices. Our results suggest that AHL-degrading activity of ahlS might perform useful functions such as useful biocontrol agents...
  48. pmc An outer membrane enzyme encoded by Salmonella typhimurium lpxR that removes the 3'-acyloxyacyl moiety of lipid A
    C Michael Reynolds
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 281:21974-87. 2006
    ..coli O157:H7, Yersinia enterocolitica, Helicobacter pylori, and Vibrio cholerae. The function of LpxR is unknown, but it could play a role in pathogenesis because it might modulate the cytokine response of an infected animal...
  49. pmc A physiological connection between tmRNA and peptidyl-tRNA hydrolase functions in Escherichia coli
    Nongmaithem Sadananda Singh
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India
    Nucleic Acids Res 32:6028-37. 2004
    ..We discuss the physiological relevance of these observations to highlight a major role of tmRNA in decreasing cellular peptidyl-tRNA load...
  50. ncbi Depot-specific expression of lipolytic genes in human adipose tissues--association among CES1 expression, triglyceride lipase activity and adiposity
    Shuichi Nagashima
    Division of Endocrinology and Metabolism, Diabetes Center, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
    J Atheroscler Thromb 18:190-9. 2011
    ....
  51. ncbi A host lipase detoxifies bacterial lipopolysaccharides in the liver and spleen
    Baomei Shao
    Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, Texas 75390 9113, USA
    J Biol Chem 282:13726-35. 2007
    ..AOAH-mediated deacylation is a previously unappreciated mechanism that prevents prolonged inflammatory reactions to Gram-negative bacteria and LPS in the liver and spleen...
  52. pmc VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract
    Lixi Jiang
    Institute of Molecular and Cell Biology, Singapore 138673
    Plant Cell 17:584-96. 2005
    ..Our study suggests that the VGD1 product is required for growth of the pollen tube, possibly via modifying the cell wall and enhancing the interaction of the pollen tube with the female style and transmitting tract tissues...
  53. ncbi The ectopic expression of a pectin methyl esterase inhibitor increases pectin methyl esterification and limits fungal diseases in wheat
    Chiara Volpi
    Department of Agrobiology and Agrochemistry, University of Tuscia, Viterbo, Italy
    Mol Plant Microbe Interact 24:1012-9. 2011
    ..In addition to their importance for wheat improvement, these results highlight the primary role of pectin despite its low content in the wheat cell wall...
  54. ncbi Role of endoplasmic reticulum neutral lipid hydrolases
    Ariel D Quiroga
    Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada
    Trends Endocrinol Metab 22:218-25. 2011
    ..Some of these enzymes might be attractive targets for the treatment of dyslipidemias, viral infection and atherosclerosis...
  55. pmc Characterization of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins and their potential application in bioscouring
    Yao Zhang
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
    Appl Environ Microbiol 76:6870-6. 2010
    ..The improvement in activity and the strong synergistic effect between the fusion proteins and pectinase suggest that they may have better applications in textile bioscouring than the native cutinase...
  56. ncbi Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase
    Guenter Haemmerle
    Institute of Molecular Biosciences, University of Graz, Austria
    Science 312:734-7. 2006
    ..These results indicate that ATGL is rate limiting in the catabolism of cellular fat depots and plays an important role in energy homeostasis...
  57. ncbi Polymorphisms in the novel gene acyloxyacyl hydroxylase (AOAH) are associated with asthma and associated phenotypes
    Kathleen C Barnes
    Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 118:70-7. 2006
    ....
  58. ncbi Methyl esterification of pectin plays a role during plant-pathogen interactions and affects plant resistance to diseases
    Vincenzo Lionetti
    Dipartimento di Biologia e Biotecnologie Charles Darwin, Sapienza Universita di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy
    J Plant Physiol 169:1623-30. 2012
    ..This review focuses on the impact of pectin methyl esterification in plant-pathogen interactions and on the dynamic role of its alteration during pathogenesis...
  59. ncbi Role of the leader sequence in tobacco pectin methylesterase secretion
    Yuri L Dorokhov
    Department of Virology, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Leninsky Gory 1, Laboratory Building A, Moscow 119992, Russia
    FEBS Lett 580:3329-34. 2006
    ..The CW targeting was abolished by various deletions in the TM domain, i.e., anchor domain was essential for secretion of GFP to CW. By contrast, even entire deletion of the spacer segment had no influence on GFP targeting...
  60. pmc Pectin methylesterase, a regulator of pollen tube growth
    Maurice Bosch
    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Plant Physiol 138:1334-46. 2005
    ....
  61. ncbi Specificity and enzyme kinetics of the quorum-quenching N-Acyl homoserine lactone lactonase (AHL-lactonase)
    Lian Hui Wang
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    J Biol Chem 279:13645-51. 2004
    ..We conclude that AHL-lactonase is a highly specific enzyme and that the (106)HXDH(109) approximately H(169) of AHL-lactonase represents a novel catalytic motif, which does not rely on zinc or other metal ions for activity...
  62. pmc Regulation of triglyceride metabolism. IV. Hormonal regulation of lipolysis in adipose tissue
    Kathy Jaworski
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Am J Physiol Gastrointest Liver Physiol 293:G1-4. 2007
    ..In this review, we summarize and discuss the recent progress made in the regulation of adipocyte lipolysis...
  63. pmc Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis
    Ricky L Ulrich
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702, USA
    Appl Environ Microbiol 70:6173-80. 2004
    ..AiiA lactonases in B. thailandensis reduced AHL accumulation, affected both swarming and twitching motility, increased generation time, altered substrate utilization, and prevented the beta-hemolysis of sheep erythrocytes...
  64. pmc A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa
    S Wilhelm
    Lehrstuhl Biologie der Mikroorganismen, Ruhr Universitat, D 44780 Bochum, Germany
    J Bacteriol 181:6977-86. 1999
    ..To our knowledge, this esterase is unique in that it exemplifies in P. aeruginosa (i) the first enzyme identified in the outer membrane and (ii) the first example of a type IV secretion mechanism...
  65. ncbi Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens
    Alessandro Raiola
    Department of Land, Environment, Agriculture and Forestry, University of Padua, Legnaro PD Italy
    Mol Plant Microbe Interact 24:432-40. 2011
    ..We show that, upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue...
  66. ncbi Peptidyl-tRNA hydrolase and its critical role in protein biosynthesis
    Gautam Das
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
    Microbiology 152:2191-5. 2006
    ..This review discusses the aspects of Pth that relate to its history and biochemistry and its physiological connections with various cellular factors...
  67. ncbi Intestinal absorption of dietary cholesteryl ester is decreased but retinyl ester absorption is normal in carboxyl ester lipase knockout mice
    W Weng
    Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University, New York 10021, USA
    Biochemistry 38:4143-9. 1999
    ..Overall, the data indicate that in the mouse, other enzymes besides CEL participate in the hydrolysis of dietary cholesteryl esters, retinyl esters, and triglycerides...
  68. ncbi Evolution of supergene families associated with insecticide resistance
    Hilary Ranson
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
    Science 298:179-81. 2002
    ..The analyses also provide the means for identifying previously unknown molecules involved in fundamental biological processes such as development...
  69. ncbi A metagenomic analysis of soil bacteria extends the diversity of quorum-quenching lactonases
    Kashif Riaz
    Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, UPR2355, Gif sur Yvette, France
    Environ Microbiol 10:560-70. 2008
    ..coli. Phylogenetic analysis showed that 9 orf out of 20 were related to sequences from members of the Acidobacteria, supporting the hypothesis that the analysed insert might be originated from an organism related to this phylum...
  70. ncbi Spatial control of protein phosphatase 2A (de)methylation
    Sari Longin
    Department of Molecular Cell Biology, Faculty of Medicine, KU Leuven, Herestraat 49 bus 901, B 3000 Leuven, Belgium
    Exp Cell Res 314:68-81. 2008
    ..PME-1 associates with an inactive PP2A population, regardless of its esterase activity or localization. We propose that stabilization of this inactive, nuclear PP2A pool is a major in vivo function of PME-1...
  71. pmc Premature leaf senescence modulated by the Arabidopsis PHYTOALEXIN DEFICIENT4 gene is associated with defense against the phloem-feeding green peach aphid
    Venkatramana Pegadaraju
    Division of Biology and the Molecular Cellular and Developmental Biology Program, Kansas State University, Manhattan, Kansas 66506 4901, USA
    Plant Physiol 139:1927-34. 2005
    ..Our results suggest that PAD4 modulates the activation of senescence in the aphid-infested leaves, which contributes to basal resistance to GPA...
  72. ncbi Quorum sensing and quorum-quenching enzymes
    Yi Hu Dong
    Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673
    J Microbiol 43:101-9. 2005
    ....
  73. ncbi GDSL lipase-like 1 regulates systemic resistance associated with ethylene signaling in Arabidopsis
    Sun Jae Kwon
    School of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
    Plant J 58:235-45. 2009
    ..Our results demonstrate that GLIP1-elicited systemic resistance is dependent on ethylene signaling and provide evidence that GLIP1 may mediate the production of a systemic signaling molecule(s)...
  74. pmc Extremely stable and versatile carboxylesterase from a hyperthermophilic archaeon
    Yuji Hotta
    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Appl Environ Microbiol 68:3925-31. 2002
    ..The extreme stability against heat and organic solvents, along with its activity towards a tertiary alcohol ester, indicates a high potential for the Est of P. calidifontis in future applications...
  75. ncbi Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca)
    Sonia Osorio
    Departamento de Biologia Molecular y Bioquimica, Universidad de Malaga, 29071 Malaga, Spain
    Plant J 54:43-55. 2008
    ..Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged...
  76. ncbi Juvenile hormone esterase activity in the pupating and diapausing larvae of Sesamia nonagrioides
    Christa Schafellner
    Department of Forest Entomology, Forest Pathology, and Forest Protection, University of Natural Resources and Applied Life Sciences, Hasenauerstrasse 38, A 1190 Vienna, Austria
    J Insect Physiol 54:916-21. 2008
    ..The data indicate that JHE is not crucial for the switch between larval development, diapause, and metamorphosis in S. nonagrioides...
  77. ncbi Patatins, Kunitz protease inhibitors and other major proteins in tuber of potato cv. Kuras
    Guy Bauw
    Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Denmark
    FEBS J 273:3569-84. 2006
    ..Furthermore, protein abundance was contrasted with the levels of corresponding gene transcripts found in our previous EST and LongSAGE studies of Kuras tuber...
  78. pmc Inhibition of Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation by aminoarabinose membrane modification
    Kiyoshi Kawasaki
    Department of Microbiology, University of Washington, Health Sciences Building, K140, Box 357710, 1959 Pacific St N E, Seattle, WA 98195, USA
    J Bacteriol 187:2448-57. 2005
    ..Taken together, these results suggest that PagL enzymatic deacylation is posttranslationally inhibited by membrane environments, which either sequester PagL from its substrate or alter its conformation...
  79. ncbi Purification and characterization of an esterase hydrolyzing monoalkyl phthalates from Micrococcus sp. YGJ1
    Kiyofumi Maruyama
    Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501 1193, Japan
    J Biochem 137:27-32. 2005
    ..These findings show that the enzymes bear a close resemblance to the putative phthalate ester hydrolase (PehA) of Arthrobacter keyseri 12B...
  80. pmc Pectin methylesterase NaPME1 contributes to the emission of methanol during insect herbivory and to the elicitation of defence responses in Nicotiana attenuata
    Evelyn Körner
    Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans Knöll Str 8, D 07745 Jena, Germany
    J Exp Bot 60:2631-40. 2009
    ..These latter responses could not be recovered by application of exogenous MeOH. Together, these results indicated that PME contributes, probably indirectly by affecting cell wall properties, to the induction of anti-herbivore responses...
  81. pmc Senescence marker protein 30 functions as gluconolactonase in L-ascorbic acid biosynthesis, and its knockout mice are prone to scurvy
    Yoshitaka Kondo
    Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173 0015, Japan
    Proc Natl Acad Sci U S A 103:5723-8. 2006
    ..6% of those in WT control mice. In addition, by using the SMP30 knockout mouse, we demonstrate that the alternative pathway of AA synthesis involving d-glucurono-gamma-lactone operates in vivo, although its flux is fairly small...
  82. ncbi Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae
    Takuya Koseki
    Department of Bioresource Engineering, Yamagata University, Tsuruoka, Japan
    Appl Microbiol Biotechnol 83:689-96. 2009
    ..Ferulic acid was efficiently released from wheat arabinoxylan when both esterases were applied with xylanase from Thermomyces lanuginosus. Both recombinant proteins also exhibited hydrolytic activity toward chlorogenic acid...
  83. ncbi Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: probable identity with brush border phospholipase B
    K M Rigtrup
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232 0146
    Biochemistry 33:2661-6. 1994
    ..Vmax values were also bile salt dependent. Retinyl palmitate was hydrolyzed most rapidly in the presence of deoxycholate and least rapidly in taurocholate.(ABSTRACT TRUNCATED AT 250 WORDS)..
  84. ncbi Purification and characterization of an extracellular feruloyl esterase from the thermophilic anaerobe Clostridium stercorarium
    J A Donaghy
    Food Science Division Microbiology, Department of Agriculture for Northern Ireland, Belfast, UK
    J Appl Microbiol 88:458-66. 2000
    ..0 and 65 degrees C. At a concentration of 5 mmol l-l, the ions Ca2+, Cu2+, Co2+ and Mn2+ reduced the activity by 70-80%...
  85. ncbi Cloning and expression of the cutinase A gene of Botrytis cinerea
    C J van der Vlugt-Bergmans
    Department of Phytopathology, Wageningen Agricultural University, The Netherlands
    Mol Plant Microbe Interact 10:21-9. 1997
    ..The expression of cutA during infection of tomato leaves is low during early phases of infection, but high when the fungus has colonized the leaf and starts to sporulate...
  86. pmc Overproduction of acyloxyacyl hydrolase by macrophages and dendritic cells prevents prolonged reactions to bacterial lipopolysaccharide in vivo
    Noredia Ojogun
    Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, TX, USA
    J Infect Dis 200:1685-93. 2009
    ..Our results suggest that the extent to which macrophages and dendritic cells produce AOAH may influence the outcome of many gram-negative bacterial diseases...
  87. pmc Activity-based protein profiling identifies a host enzyme, carboxylesterase 1, which is differentially active during hepatitis C virus replication
    David R Blais
    Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada
    J Biol Chem 285:25602-12. 2010
    ..In transgenic mice containing human-mouse chimeric livers, HCV infection also correlates with higher levels of endogenous CES1, providing further evidence that CES1 has an important role in HCV propagation...
  88. pmc Host inactivation of bacterial lipopolysaccharide prevents prolonged tolerance following gram-negative bacterial infection
    Mingfang Lu
    Infectious Disease Division, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 9113, USA
    Cell Host Microbe 4:293-302. 2008
    ..coli challenge. Inactivating LPS, an immunostimulatory microbial molecule, is thus important for restoring effective host defenses following Gram-negative bacterial infection in animals...
  89. pmc Identification and characterization of bacterial cutinase
    Sheng Chen
    State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi, Jiangsu 214122, China
    J Biol Chem 283:25854-62. 2008
    ..This is the first report of cutinase encoding genes from bacterial sources...
  90. ncbi All-trans-retinyl esters are the substrates for isomerization in the vertebrate visual cycle
    Deviprasad R Gollapalli
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 45 Shattuck Street, Boston, Massachusetts 02115, USA
    Biochemistry 42:5809-18. 2003
    ..The converse is true with vitamin A. These data unambiguously establish all-trans-retinyl esters as the precursors of 11-cis-retinol...
  91. ncbi Phloem-based resistance to green peach aphid is controlled by Arabidopsis PHYTOALEXIN DEFICIENT4 without its signaling partner ENHANCED DISEASE SUSCEPTIBILITY1
    Venkatramana Pegadaraju
    Division of Biology and Molecular Cellular and Developmental Biology Program, Kansas State University, Manhattan, KS 66506, USA
    Plant J 52:332-41. 2007
    ..Thus, a PAD4 mode of action that is uncoupled from EDS1 determines the extent of aphid feeding in the phloem...
  92. ncbi Peptidyl-tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable
    Jeanne Menez
    UPR 9073 du CNRS, Institut de Biologie Physico Chimique, Paris, France
    Mol Microbiol 45:123-9. 2002
    ....
  93. ncbi Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells
    Tomohisa Hasunuma
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1, Yamadaoka, Suita 565 0871, Japan
    J Biotechnol 106:45-52. 2003
    ..7% compared to the vector control transformant. This is the first report of methanol overproduction in plant cells by means of genetic engineering...
  94. ncbi Endotoxin-binding proteins modulate the susceptibility of bacterial endotoxin to deacylation by acyloxyacyl hydrolase
    Theresa L Gioannini
    Inflammation Program, Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 282:7877-84. 2007
    ..The susceptibility of the monomeric endotoxin-CD14 complex to AOAH may help constrain endotoxin-induced TLR4 activation when endotoxin and membrane CD14 are present in excess of MD-2/TLR-4...
  95. pmc Retinal pigment epithelium-retinal G protein receptor-opsin mediates light-dependent translocation of all-trans-retinyl esters for synthesis of visual chromophore in retinal pigment epithelial cells
    Roxana A Radu
    Jules Stein Eye Institute, California, USA
    J Biol Chem 283:19730-8. 2008
    ..This translocation permits insoluble all-trans-retinyl esters to be utilized as substrate for the synthesis of a new visual chromophore...
  96. ncbi Molecular screening for GS2 lipase regulators: inhibition of keratinocyte retinylester hydrolysis by TIP47
    Jay G Gao
    Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, USA
    J Invest Dermatol 126:2087-95. 2006
    ..Residues within the carboxyl alpha3-alpha4 helices are essential in the context of the full-length protein. Residues within the amino-terminal also contribute depending on the context...
  97. ncbi Autotransporters with GDSL passenger domains: molecular physiology and biotechnological applications
    Susanne Wilhelm
    Institute of Molecular Enzyme Technology, Heinrich Heine University Dusseldorf, Research Center Julich, Juelich, Germany
    Chembiochem 12:1476-85. 2011
    ..Furthermore, it is capable of displaying different classes of enzymes in a range of Gram-negative bacteria including Escherichia coli, and FACS-based high-throughput screening for enantioselective esterases could be achieved using EstA...
  98. pmc Use of Pseudomonas putida EstA as an anchoring motif for display of a periplasmic enzyme on the surface of Escherichia coli
    Taek Ho Yang
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1, Guseong Dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 70:6968-76. 2004
    ..coli. This investigation also provides a possible topological model of the EstA translocator domain, which might serve as a basis for the construction of fusion proteins containing heterologous passenger domains...
  99. ncbi Occurrence, properties, and applications of feruloyl esterases
    Takuya Koseki
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, 1 23 Wakaba machi, Tsuruoka 997 8555, Japan
    Appl Microbiol Biotechnol 84:803-10. 2009
    ..An overview of the current knowledge of fungal feruloyl esterases focusing on the diverse of substrate specificity and potential applications is presented in this review. Furthermore, biological functions of ferulic acid are discussed...
  100. pmc Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter
    Toon Nicolay
    Centre of Microbial and Plant Genetics, KU Leuven, Heverlee, Belgium
    Appl Environ Microbiol 78:2533-42. 2012
    ..No specific phenotype was detected for an estA-negative mutant. Overall, P. stutzeri A15 EstA is a new candidate for the surface display of proteins in environmentally relevant biotechnological applications...
  101. pmc Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population: a replication study
    Yuan Zhang
    Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, People s Republic of China
    PLoS ONE 7:e39247. 2012
    ..The development of CRS is believed to be the result of combined interactions between the genetic background of the affected subject and environmental factors...

Research Grants39

  1. SELECTIVE THERAPY OF TUMORS THAT OVEREXPRESS C MYC
    PHILIP POTTER; Fiscal Year: 2006
    ..These studies will assess the effectiveness of VDEPT with rCE or hiCE and CPT-11 at eliminating tumor cells from appropriate animal models of human disease. ..
  2. Novel Therapeutic Approaches for Narcotic Overdose
    PHILIP POTTER; Fiscal Year: 2009
    ..Overall, these studies should allow development of novel therapeutic approaches for the treatment of cocaine overdose. ..
  3. Analysis of Therapeutic Adipose Tissue Remodeling
    JAMES GRANNEMAN; Fiscal Year: 2004
    ..2) To examine adipose tissue cellular dynamics during therapeutic remodeling induced by agonist activation of beta3-adrenergic receptors and PPAR-gamma. ..
  4. Trihalomethane Pharmacokinetics and Pharmacodynamics
    MATTHEW ROSS; Fiscal Year: 2003
    ..It is also essential to determine if the same metabolic pathways are operative in rodents and humans and if metabolic flux through the different pathways is similar. ..
  5. Structure and Function of the Human Pregnane X Receptor
    MATTHEW REDINBO; Fiscal Year: 2006
    ..5. Elucidate structures of hPXR in complexes with large drugs like rifampicin and taxol. 6. Examine structures of mouse and rabbit PXR to determine why different species respond to distinct xenobiotics. ..
  6. BETA 1 AND BETA 3 ADRENORECEPTORS
    JAMES GRANNEMAN; Fiscal Year: 2001
    ..Aim 2. To further characterize the differential signaling properties of beta1- beta3-AR and to examine the cellular and molecular basis. Specific Aim 3. To examine the molecular pharmacology of beta3-AR-selective agonists. ..
  7. Structure and Mechanism of Human Topoisomerase I
    MATTHEW REDINBO; Fiscal Year: 2004
    ....
  8. Evaluation in Primates of Cocaine Esterase for the Treatment of Cocaine Toxicity
    Mei Chuan Ko; Fiscal Year: 2010
    ..Dose-response functions for each enzyme will be obtained repeatedly and blood samples analyzed for titer development so that tolerance to the effects of CocE can be monitored. ..
  9. Intrathecal Opioid-Induced Pruritus
    Mei Chuan Ko; Fiscal Year: 2003
    ..These studies would provide a systematic understanding of the pharmacology of intrathecal morphine-induced pruritus in primates and establish the basis for identifying potential therapeutically effective antipruritics. ..
  10. Contribution of ATGL in skeletal muscle to lipid metabolism and insulin action
    ERIN KERSHAW; Fiscal Year: 2008
    ..Evaluating the role of ATGL-mediated fat breakdown in skeletal muscle will provide important insights into the pathophysiology and treatment of obesity, insulin resistance, and related metabolic disorders. [unreadable] [unreadable]..
  11. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN RAETZ; Fiscal Year: 2008
    ..leguminosarum lipid A; and IV) i characterization of enzymes that incorporate the unique inner core sugars of/?, leguminosarum LPS. ..
  12. AIRWAY DISEASE AND COCKROACH EXPOSURE IN PUBLIC HOUSING
    Kathleen Barnes; Fiscal Year: 2008
    ..We hope that with the data to be generated, we will positively impact the health of urban US populations who are disproportionally suffering from asthma morbidity and mortality. ..
  13. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
    ..We believe that an understanding of nuclear lipid signaling is key to our ability to intervene in events such as wound repair and tissue remodeling. ..
  14. INTERACTIONS OF ENDOTOXIN WITH PLASMA AND CELLS
    Robert Munford; Fiscal Year: 2007
    ..abstract_text> ..
  15. Carboxyl Ester Lipase Affects Hepatic HDL Metabolism
    PHILIP HOWLES; Fiscal Year: 2009
    ....
  16. Genome-Wide Association Studies of Asthma in Populations of African Descent
    Kathleen Barnes; Fiscal Year: 2008
    ..Findings from this study will provide a better understanding of the complex pathways related to risk of asthma and associated phenotypes. [unreadable] [unreadable] [unreadable]..
  17. Neural Progenitor Cells as Cancer Therapy Vectors
    Karen Aboody; Fiscal Year: 2009
    ..The described approach might then be adapted to the treatment of other solid tumors by careful choice of appropriate therapeutic transgenes. ..
  18. Light & Heavy Alcohol, Paraoxonase & Oxidized LDL Status
    Raj Lakshman; Fiscal Year: 2008
    ..These investigations are likely to lead to new insights in our understanding of the cardioprotective effects of ligh drinking and proatherogenic effects of heavy drinking both at clinical and at molecular levels. ..
  19. Clinical Features in Nursing Home Residents with Suspected UTI
    Manisha Juthani Mehta; Fiscal Year: 2008
    ..This study will create an evidence-based definition of UTI. Future studies will identify residents with UTI in whom antibiotic therapy improves clinical outcome. [unreadable] [unreadable] [unreadable]..
  20. The Effect of a Mixture of Pesticides on the Rat Cardiac Proteome
    John Crow; Fiscal Year: 2007
    ..Our long-term objective is to better understand the extent to which commonly used pesticides and other environmental chemicals influence heart disease and its treatment. [unreadable] [unreadable] [unreadable]..
  21. MECHANISMS OF ACTIONS OF ALCOHOL AND OMEGA 3-FATTY ACIDS
    Raj Lakshman; Fiscal Year: 2004
    ....
  22. Mechanism and Engineering of an Autoinducer Hydrolase
    Walter Fast; Fiscal Year: 2004
    ..If successful, the incremental truncation technique used to create these hybrids may be applicable for "humanizing" other therapeutically useful bacterial enzymes. ..
  23. ENDOGENOUSLY REGULATED IMMUNOMODULATION
    Robert Munford; Fiscal Year: 2003
    ..abstract_text> ..
  24. Phospholipases in the Pathogenesis of M. tuberculosis
    Sarah Parker; Fiscal Year: 2006
    ..Dr. Parker is committed to research in M. tuberculosis, and it holds a special significance to her because of her life experiences. ..
  25. The Acquisition of Bacterial Vaginosis in Lesbians
    Jeanne Marrazzo; Fiscal Year: 2006
    ..Lesbian couples provide a unique opportunity to conduct comparative studies of vaginal microbial ecology in sex partners, and to directly analyze determinants of transmission. ..
  26. Genetic Studies of Immune Responsiveness to Cockroach
    Kathleen Barnes; Fiscal Year: 2007
    ..We will also examine the interactive effect of current cockroach allergen exposure and susceptibility loci associated with asthma severity. ..
  27. CAROTENOID BINDING PROTEIN AND CAROTENOID METABOLISM
    Raj Lakshman; Fiscal Year: 2002
    ..2. Molecular biology techniques to measure CCBP mRNA levels in different tissues and to clone its cDNA. 3. HPLC procedures for quantification of various carotenoids and retinoids. ..
  28. in vivo characterization of D2/D3 agonists & antagonists
    Gregory Collins; Fiscal Year: 2007
    ..abstract_text> ..
  29. Role of NPC1L1 in regulating energy metabolism, nutrient utilization & weight gai
    Philip N Howles; Fiscal Year: 2010
    ....
  30. MEMBRANE IMMUNOGLOBULIN ASSEMBLY AND FUNCTION
    Shiv Pillai; Fiscal Year: 2002
    ..Since the majority of human lymphomas develop form activated B-cells, these signaling mechanisms are likely to be of direct relevance for a significant proportion of human cancers. ..
  31. Marginal zone B lymphocytes and blood borne pathogens
    Shiv Pillai; Fiscal Year: 2004
    ..Finally we will attempt to determine whether antigen-specific MZ B cells, as opposed to follicuar B cells, can readily capture and present blood borne antigens, and thus activate na'fve T cells. ..
  32. Hydrophobics transport across the outer membrane
    Bert van den Berg; Fiscal Year: 2009
    ..These structures will also allow us to determine whether mechanistic differences exist between LCFA and xenobiotics transport. ..
  33. Role of PP2A in tauopathies
    Estelle Sontag; Fiscal Year: 2007
    ..If our model is validated, dietary folate supplementation and PPMT may be novel therapeutic targets for strategies aimed at slowing down the disease process in AD, and possibly other neurodegenerative dementias. [unreadable] [unreadable]..
  34. The role of PKK in NFkappa B activation
    Shiv Pillai; Fiscal Year: 2007
    ..PKK represents a novel enzyme that may be critical in pathways that are important in cancer, inflammation, cell survival, and differentiation. ..
  35. Molecular identification of a sialyl 9-O-acetyl transferase
    Shiv Pillai; Fiscal Year: 2008
    ..The gene that codes for an enzyme that controls the strength of the immune response will be studied. This gene may be an important target for the treatment of lupus and other related diseases. [unreadable] [unreadable] [unreadable]..
  36. Cell Signaling, PXR Phosphorylation & Drug Disposition
    Jeffrey Staudinger; Fiscal Year: 2009
    ..These studies will provide additional insight into the regulation of drug disposition and drug-drug interaction. ..
  37. Studies on human peripheral B lymphocytes
    Shiv Pillai; Fiscal Year: 2005
    ....
  38. Role of PP2A in tauopathies
    Estelle Sontag; Fiscal Year: 2006
    ....