amidohydrolases

Summary

Top Publications

  1. ncbi Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase
    Manuel Romero
    Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago, Spain
    FEMS Microbiol Lett 280:73-80. 2008
  2. pmc Selective blockade of 2-arachidonoylglycerol hydrolysis produces cannabinoid behavioral effects
    Jonathan Z Long
    The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 5:37-44. 2009
  3. ncbi Impaired nitric oxide synthase pathway in diabetes mellitus: role of asymmetric dimethylarginine and dimethylarginine dimethylaminohydrolase
    Ken Y Lin
    Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif 94305, USA
    Circulation 106:987-92. 2002
  4. pmc Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1
    Charles F Sio
    Pharmaceutical Biology, University Centre for Pharmacy, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Infect Immun 74:1673-82. 2006
  5. ncbi Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes
    Yi Han Lin
    Laboratory of Biosignals and Bioengineering, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Microbiol 47:849-60. 2003
  6. ncbi Structure and function of fatty acid amide hydrolase
    Michele K McKinney
    Departments of Cell Biology and Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biochem 74:411-32. 2005
  7. pmc Dysregulation of the peripheral and adipose tissue endocannabinoid system in human abdominal obesity
    Matthias Bluher
    University of Leipzig, Medical Department III, Philipp Rosenthal Str 27, 04103 Leipzig, Germany
    Diabetes 55:3053-60. 2006
  8. ncbi Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides
    B F Cravatt
    Department of Chemistry, The Scripps Research Institute, La Jolla, California 92307, USA
    Nature 384:83-7. 1996
  9. pmc Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1
    Jean J Huang
    Biology, W M Keck Laboratories, M C 138 78, California Institute of Technology, Pasadena, California 91125, USA
    Appl Environ Microbiol 72:1190-7. 2006
  10. pmc Selective N-acylethanolamine-hydrolyzing acid amidase inhibition reveals a key role for endogenous palmitoylethanolamide in inflammation
    Carlos Solorzano
    Department of Pharmacology, University of California, Irvine, CA 92697 4624, USA
    Proc Natl Acad Sci U S A 106:20966-71. 2009

Research Grants

Detail Information

Publications276 found, 100 shown here

  1. ncbi Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase
    Manuel Romero
    Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago, Spain
    FEMS Microbiol Lett 280:73-80. 2008
    ....
  2. pmc Selective blockade of 2-arachidonoylglycerol hydrolysis produces cannabinoid behavioral effects
    Jonathan Z Long
    The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 5:37-44. 2009
    ..These data indicate that 2-AG endogenously modulates several behavioral processes classically associated with the pharmacology of cannabinoids and point to overlapping and unique functions for 2-AG and anandamide in vivo...
  3. ncbi Impaired nitric oxide synthase pathway in diabetes mellitus: role of asymmetric dimethylarginine and dimethylarginine dimethylaminohydrolase
    Ken Y Lin
    Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif 94305, USA
    Circulation 106:987-92. 2002
    ..An endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), is elevated in patients with type 2 diabetes mellitus (DM). This study explored the mechanisms by which ADMA becomes elevated in DM...
  4. pmc Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1
    Charles F Sio
    Pharmaceutical Biology, University Centre for Pharmacy, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Infect Immun 74:1673-82. 2006
    ..The quorum-quenching AHL acylase may enable P. aeruginosa PAO1 to modulate its own quorum-sensing-dependent pathogenic potential and, moreover, offers possibilities for novel antipseudomonal therapies...
  5. ncbi Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes
    Yi Han Lin
    Laboratory of Biosignals and Bioengineering, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Microbiol 47:849-60. 2003
    ..Thus, AHL-acylases have fundamental implications and hold biotechnological promise in quenching quorum sensing...
  6. ncbi Structure and function of fatty acid amide hydrolase
    Michele K McKinney
    Departments of Cell Biology and Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biochem 74:411-32. 2005
    ..These studies, as well as their biological and therapeutic implications, are the subject of this review...
  7. pmc Dysregulation of the peripheral and adipose tissue endocannabinoid system in human abdominal obesity
    Matthias Bluher
    University of Leipzig, Medical Department III, Philipp Rosenthal Str 27, 04103 Leipzig, Germany
    Diabetes 55:3053-60. 2006
    ..Thus, the endocannabinoid system may represent a primary target for the treatment of abdominal obesity and associated metabolic changes...
  8. ncbi Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides
    B F Cravatt
    Department of Chemistry, The Scripps Research Institute, La Jolla, California 92307, USA
    Nature 384:83-7. 1996
    ..Therefore we will hereafter refer to oleamide hydrolase as fatty-acid amide hydrolase, in recognition of the plurality of fatty-acid amides that the enzyme can accept as substrates...
  9. pmc Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1
    Jean J Huang
    Biology, W M Keck Laboratories, M C 138 78, California Institute of Technology, Pasadena, California 91125, USA
    Appl Environ Microbiol 72:1190-7. 2006
    ..It is not yet known under which "natural" conditions quiP is expressed or how P. aeruginosa balances the expression of its quorum-sensing systems with the expression of its acyl-HSL acylase activities...
  10. pmc Selective N-acylethanolamine-hydrolyzing acid amidase inhibition reveals a key role for endogenous palmitoylethanolamide in inflammation
    Carlos Solorzano
    Department of Pharmacology, University of California, Irvine, CA 92697 4624, USA
    Proc Natl Acad Sci U S A 106:20966-71. 2009
    ..The PEA-hydrolyzing amidase NAAA may provide a previously undescribed target for antiinflammatory medicines...
  11. pmc Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases
    J M Leiper
    Centre for Clinical Pharmacology, The Rayne Institute, University College London, 5 University Street, London, U K
    Biochem J 343:209-14. 1999
    ..These results strengthen the hypothesis that methylarginine concentration is actively regulated and identify molecular targets for the tissue and cell-specific regulation of methylarginine concentration...
  12. pmc Utilization of acyl-homoserine lactone quorum signals for growth by a soil pseudomonad and Pseudomonas aeruginosa PAO1
    Jean J Huang
    Department of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Appl Environ Microbiol 69:5941-9. 2003
    ....
  13. ncbi Cardiovascular biology of the asymmetric dimethylarginine:dimethylarginine dimethylaminohydrolase pathway
    Patrick Vallance
    BHF Laboratories, Division of Medicine, University College London, Rayne Institute, 5 University St, London WC1 6JJ, UK
    Arterioscler Thromb Vasc Biol 24:1023-30. 2004
    ..This article describes the biology of ADMA and the implications for cardiovascular physiology and pathophysiology...
  14. pmc Identification of extracellular N-acylhomoserine lactone acylase from a Streptomyces sp. and its application to quorum quenching
    Sun Yang Park
    R and D Center, inBioNET Corporation, Daejeon 305 390, Korea
    Appl Environ Microbiol 71:2632-41. 2005
    ..aeruginosa. Accordingly, these results suggest that AHL-acylase, AhlM could be effectively applied to the control of AHL-mediated pathogenicity...
  15. ncbi The emerging role of asymmetric dimethylarginine as a novel cardiovascular risk factor
    Rainer H Boger
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, Center of Experimental Medicine, University Hospital Hamburg Eppendorf, D 20246 Hamburg, Germany
    Cardiovasc Res 59:824-33. 2003
    ..Among other treatments, the administration of L-arginine has been shown to improve endothelium-dependent vascular function in subjects with high ADMA levels...
  16. ncbi N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities
    Stephane Uroz
    Interactions Plantes et Micro organismes de la Rhizosphère, Institut des Sciences du Vegetal, CNRS, Batiment 23, 91198 Gif sur Yvette Cedex, France
    Microbiology 151:3313-22. 2005
    ..R. erythropolis is therefore capable of modifying and degrading AHL signal molecules through both oxidoreductase and amidolytic activities...
  17. ncbi Modulation of anxiety through blockade of anandamide hydrolysis
    Satish Kathuria
    Department of Pharmacology, University of California, Irvine, California, USA
    Nat Med 9:76-81. 2003
    ..Our results indicate that anandamide participates in the modulation of emotional states and point to fatty acid amide hydrolase inhibition as an innovative approach to anti-anxiety therapy...
  18. ncbi Analysis of functions of the chitin deacetylase gene family in Tribolium castaneum
    Yasuyuki Arakane
    Department of Biochemistry, Kansas State University, Manhattan, 66506, USA
    Insect Biochem Mol Biol 39:355-65. 2009
    ..These results reveal functional specialization among T. castaneum CDA genes and splice variants...
  19. ncbi Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group
    Manuel Romero
    Departamento de Microbiologia, Facultad de Biología CIBUS, Universidad de Santiago de Compostela, Santiago, Spain
    FEMS Microbiol Lett 304:131-9. 2010
    ..maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance...
  20. ncbi A role for monoglyceride lipase in 2-arachidonoylglycerol inactivation
    Thien P Dinh
    Department of Pharmacology, University of California, 360 Med Surge II, Irvine, CA 92697 4625, USA
    Chem Phys Lipids 121:149-58. 2002
    ..These results indicate that hydrolysis via MGL may be a primary route of 2-AG inactivation in intact neuronal cells...
  21. ncbi Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus
    A Scorpio
    Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Nat Med 2:662-7. 1996
    ..tuberculosis and also for rapid differentiation of M. bovis from M. tuberculosis strains...
  22. ncbi Protein N-terminal methionine excision
    C Giglione
    Protein Maturation Group, Institut des Sciences du Vegetal, UPR2355, Centre National de la Recherche Scientifique, Batiment 23, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Cell Mol Life Sci 61:1455-74. 2004
    ..Such inhibitors have considerable potential for use in the treatment of various human diseases, from cancer to bacterial and parasitic infections...
  23. ncbi The curious characteristics of pyrazinamide: a review
    Y Zhang
    Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Int J Tuberc Lung Dis 7:6-21. 2003
    ..Methods for susceptibility determination either as tests against PZA or nicotinamide in liquid and solid media, as tests for PZase activity or for mutations in pncA, are reviewed...
  24. ncbi Molecular characterization of N-acylethanolamine-hydrolyzing acid amidase, a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase
    Kazuhito Tsuboi
    Department of Biochemistry, Kagawa University School of Medicine, 1750 1 Ikenobe, Miki, Kagawa 761 0793, Japan
    J Biol Chem 280:11082-92. 2005
    ..These results demonstrated that NAAA is a novel N-acylethanolamine-hydrolyzing enzyme that shows structural and functional similarity to acid ceramidase...
  25. pmc A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system
    Ivo G Boneca
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75724 Paris, France
    Proc Natl Acad Sci U S A 104:997-1002. 2007
    ..The presence of deacetylase genes in other pathogenic bacteria indicates that PG N-deacetylation could be a general mechanism used by bacteria to evade the host innate immune system...
  26. ncbi Dimethylarginine dimethylaminohydrolase (DDAH): expression, regulation, and function in the cardiovascular and renal systems
    Fredrik Palm
    Division of Nephrology and Hypertension, Georgetown University, 3800 Reservoir Road N W, Washington, DC 20007, USA
    Am J Physiol Heart Circ Physiol 293:H3227-45. 2007
    ....
  27. ncbi serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila
    Stefan Luschnig
    Bayreuther Zentrum für Molekulare Biowissenschaften, Department of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany
    Curr Biol 16:186-94. 2006
    ....
  28. pmc Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome
    Brian V Jones
    Alimentary Pharmabiotic Centre and Department of Microbiology, and School of Pharmacy, University College Cork, Cork, Ireland
    Proc Natl Acad Sci U S A 105:13580-5. 2008
    ....
  29. ncbi Discovery of expression QTLs using large-scale transcriptional profiling in human lymphocytes
    Harald H H Goring
    Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78245, USA
    Nat Genet 39:1208-16. 2007
    ....
  30. ncbi Localization of the endocannabinoid-degrading enzyme fatty acid amide hydrolase in rat dorsal root ganglion cells and its regulation after peripheral nerve injury
    Isobel J Lever
    Pain Research Group, Department of Anaesthetics, Pain Medicine and Intensive Care, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital Campus, London SW10 9NH, UK
    J Neurosci 29:3766-80. 2009
    ..This study reveals the location of FAAH in neural tissue involved in peripheral nociceptive transmission...
  31. pmc High-throughput identification of inhibitors of human mitochondrial peptide deformylase
    Christophe Antczak
    High Throughput Screening Core Facility, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biomol Screen 12:521-35. 2007
    ....
  32. pmc Inhibitors of fatty acid amide hydrolase reduce carrageenan-induced hind paw inflammation in pentobarbital-treated mice: comparison with indomethacin and possible involvement of cannabinoid receptors
    Sandra Holt
    Department of Pharmacology and Clinical Neuroscience, Umea University, Sweden
    Br J Pharmacol 146:467-76. 2005
    ..In conclusion, the results show that in mice, treatment with indomethacin and URB597 produce SR144528-sensitive anti-inflammatory effects in the carrageenan model of acute inflammation...
  33. ncbi Genetic analysis of a bile salt hydrolase in Bifidobacterium animalis subsp. lactis KL612
    G B Kim
    Department of Food Science and Agricultural Chemistry, McGill University, Montreal, Quebec, Canada
    J Appl Microbiol 105:778-90. 2008
    ..lactis KL612, and further analysis of the bsh promoter and an operon-like structure containing the bsh gene in the genus Bifidobacterium...
  34. ncbi Arginase induction represses gall development during clubroot infection in Arabidopsis
    Antoine Gravot
    Universite Rennes 1, UMR1349 IGEPP, F 35000 Rennes, France
    Plant Cell Physiol 53:901-11. 2012
    ..We hypothesized that arginase contributes to a negative control over clubroot symptoms, by reducing hormone-triggered cellular proliferation...
  35. pmc Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation
    D Mazel
    Unité de Physiologie Cellulaire CNRS URA 1300, Institut Pasteur, Paris, France
    EMBO J 13:914-23. 1994
    ..These findings designate deformylase as a target for antibacterial chemotherapy...
  36. pmc Blockade of endocannabinoid-degrading enzymes attenuates neuropathic pain
    S G Kinsey
    Department of Pharmacology and Toxicology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia, USA
    J Pharmacol Exp Ther 330:902-10. 2009
    ..These data indicate that inhibition of FAAH and MAGL reduces neuropathic pain through distinct receptor mechanisms of action and present viable targets for the development of analgesic therapeutics...
  37. ncbi Trypanothione synthesis in crithidia revisited
    Marcelo Comini
    Technical University of Braunschweig, Department of Biochemistry, Germany
    J Biol Chem 280:6850-60. 2005
    ....
  38. pmc Expression and function of fatty acid amide hydrolase in prostate cancer
    Michael P Endsley
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Int J Cancer 123:1318-26. 2008
    ..These results suggest that FAAH regulates 2-AG hydrolysis and invasion of prostate carcinoma cells and is potentially involved in prostate tumorigenesis...
  39. pmc Isolation of NAD cycle mutants defective in nicotinamide mononucleotide deamidase in Salmonella typhimurium
    W Cheng
    Biology Department, University of Utah, Salt Lake City 84112, USA
    J Bacteriol 177:6711-7. 1995
    ..We propose that pncD mutations reduce the activity of an ilv biosynthetic enzyme that is naturally sensitive to inhibition by NMN...
  40. pmc Identification of intracellular carriers for the endocannabinoid anandamide
    Martin Kaczocha
    Departments of Biochemistry and Cell Biology and Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 106:6375-80. 2009
    ..Taken together, FABPs represent the first proteins known to transport AEA from the plasma membrane to FAAH for inactivation and may therefore be novel pharmacological targets...
  41. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
    ..These results suggest that the stabilities of other glycosylphosphatidylinositol pathway enzymes are not dependent on GlcNAc-PI de-N-acetylase levels...
  42. pmc Identification of nicotinamide mononucleotide deamidase of the bacterial pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase family
    Luca Galeazzi
    Department of Molecular Pathology and Innovative Therapies, Section of Biochemistry, Universita Politecnica delle Marche, Ancona 60131, Italy
    J Biol Chem 286:40365-75. 2011
    ..which is available for one of the PncC family member, shows no homology with any previously described amidohydrolases. Multiple alignment analysis of functional and nonfunctional PncC homologs, together with NMN docking ..
  43. pmc Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology
    Ryan W Shepherd
    Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720 3102, USA
    Appl Environ Microbiol 75:45-53. 2009
    ..While AHL acylases might enable P. syringae B728a to degrade signals of competing species and block expression of their QS-dependent traits, these enzymes also play fundamental roles in biofilm formation...
  44. ncbi Disruption of methylarginine metabolism impairs vascular homeostasis
    James Leiper
    Centre for Clinical Pharmacology, Division of Medicine, University College London, London WC1E 6JJ, UK
    Nat Med 13:198-203. 2007
    ..Our results also suggest that DDAH inhibition could be harnessed therapeutically to reduce the vascular collapse associated with sepsis...
  45. pmc A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro
    Tatsuya Fukushima
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda shi, Nagano 386 8567, Japan
    J Bacteriol 187:1287-92. 2005
    ....
  46. ncbi Overexpression of dimethylarginine dimethylaminohydrolase reduces tissue asymmetric dimethylarginine levels and enhances angiogenesis
    Johannes Jacobi
    Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif 94305, USA
    Circulation 111:1431-8. 2005
    ....
  47. ncbi Expression and localization of the cannabinoid receptor type 1 and the enzyme fatty acid amide hydrolase in the retina of vervet monkeys
    J Bouskila
    School of Optometry, University of Montreal, Montreal, QC, Canada
    Neuroscience 202:117-30. 2012
    ..Neither CB1R nor FAAH are found in the retinal glia, the Müller cells. These data indicate that the eCB system is present throughout the primate retina and is ideally positioned to modulate central and peripheral retinal functions...
  48. pmc Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227
    J S Karns
    USDA Agricultural Research Service, Natural Resources Institute, Soil Microbial Systems Laboratory, BARC West, Beltsville, Maryland 20705 2350, USA
    Appl Environ Microbiol 65:3512-7. 1999
    ..Neither the nucleotide sequence of trzD nor the amino acid sequence of the gene product exhibited a significant level of similarity to any known gene or protein...
  49. ncbi Analysis of methylarginine metabolism in the cardiovascular system identifies the lung as a major source of ADMA
    Patrick Bulau
    Lung Center, University of Giessen School of Medicine, Aulweg 123, D 35329 Giessen, Germany
    Am J Physiol Lung Cell Mol Physiol 292:L18-24. 2007
    ..Together, these observations suggest that methylarginine metabolism by the pulmonary system significantly contributes to circulating ADMA and SDMA levels...
  50. pmc Actions of the FAAH inhibitor URB597 in neuropathic and inflammatory chronic pain models
    Angelo Jayamanne
    Pain Management Research Institute, Northern Clinical School, The University of Sydney, NSW, Australia
    Br J Pharmacol 147:281-8. 2006
    ..These findings suggest that the FAAH inhibitor URB597 produces cannabinoid CB1 and CB2 receptor-mediated analgesia in inflammatory pain states, without causing the undesirable side effects associated with cannabinoid receptor activation...
  51. ncbi Melatonin prevents increased asymmetric dimethylarginine in young rats with bile duct ligation
    You Lin Tain
    Department of Pediatrics, Chang Gung Memorial Hospital Kaohsiung Medical Center, Chang Gung University, Taiwan
    J Pineal Res 48:212-21. 2010
    ..In conclusion, melatonin therapy decreased mortality and prevented kidney injury induced by BDL via reduction of ADMA (by increasing DDAH activity) and oxidative stress...
  52. ncbi Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase
    C L Soong
    Division of Applied Life Sciences, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochem Biophys Res Commun 286:222-6. 2001
    ..The existence of a consecutive enzyme named ureidomalonase, which hydrolyzes ureidomalonic acid to urea and malonic acid, was also discovered during the purification of barbiturase...
  53. ncbi Measurement of saturable and non-saturable components of anandamide uptake into P19 embryonic carcinoma cells in the presence of fatty acid-free bovine serum albumin
    Anna Sandberg
    Department of Pharmacology and Clinical Neuroscience, Umea University, SE 901 87 Umea, Sweden
    Chem Phys Lipids 134:131-9. 2005
    ....
  54. pmc Dimethylarginine dimethylaminohydrolase-1 is the critical enzyme for degrading the cardiovascular risk factor asymmetrical dimethylarginine
    Xinli Hu
    Cardiovascular Division, Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Arterioscler Thromb Vasc Biol 31:1540-6. 2011
    ....
  55. pmc Synthesis and structure-activity relationships of N-(2-oxo-3-oxetanyl)amides as N-acylethanolamine-hydrolyzing acid amidase inhibitors
    Carlos Solorzano
    Department of Pharmacology, University of California, Irvine, 360 MSRII, Irvine, California 92697 4625, USA
    J Med Chem 53:5770-81. 2010
    ....
  56. ncbi Taurine protects against low-density lipoprotein-induced endothelial dysfunction by the DDAH/ADMA pathway
    Bin Tan
    Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Xiang Ya Road 110, Changsha 410078, China
    Vascul Pharmacol 46:338-45. 2007
    ....
  57. pmc Dissecting the essentiality of the bifunctional trypanothione synthetase-amidase in Trypanosoma brucei using chemical and genetic methods
    Susan Wyllie
    Division of Biological Chemistry and Drug Discovery, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland, UK
    Mol Microbiol 74:529-40. 2009
    ..The synthetase function of TRYS is thus unequivocally validated as a drug target by both chemical and genetic methods...
  58. ncbi Pathogenic cycle between the endogenous nitric oxide synthase inhibitor asymmetrical dimethylarginine and the leukocyte-derived hemoprotein myeloperoxidase
    Eike Christin von Leitner
    Department of Interventional Cardiology, Hamburg University Heart Center, Hamburg, Germany
    Circulation 124:2735-45. 2011
    ..The aim of the study was to investigate a potential 2-way interaction between ADMA and MPO...
  59. ncbi N-acylethanolamine metabolism with special reference to N-acylethanolamine-hydrolyzing acid amidase (NAAA)
    Natsuo Ueda
    Department of Biochemistry, Kagawa University School of Medicine, 1750 1 Ikenobe, Miki, Kagawa, Japan
    Prog Lipid Res 49:299-315. 2010
    ..In this article we discuss recent progress in the studies on the enzymes involved in the biosynthesis and degradation of NAEs with special reference to NAAA...
  60. pmc More aroused, less fatigued: fatty acid amide hydrolase gene polymorphisms influence acute response to amphetamine
    Andrea M Dlugos
    Department of Psychiatry and Behavioral Neuroscience, The University of Chicago, Chicago, IL, USA
    Neuropsychopharmacology 35:613-22. 2010
    ....
  61. ncbi Preservation of striatal cannabinoid CB1 receptor function correlates with the antianxiety effects of fatty acid amide hydrolase inhibition
    Silvia Rossi
    Clinica Neurologica, Dipartimento di Neuroscienze, Universita Tor Vergata, Via Montpellier 1, 00133 Rome, Italy
    Mol Pharmacol 78:260-8. 2010
    ..Collectively, our findings suggest that preservation of cannabinoid CB1 receptor function within the striatum is a possible synaptic correlate of the antianxiety effects of FAAH inhibition...
  62. ncbi Reduced anxiety-like behaviour induced by genetic and pharmacological inhibition of the endocannabinoid-degrading enzyme fatty acid amide hydrolase (FAAH) is mediated by CB1 receptors
    Fabricio A Moreira
    Department of Physiological Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 6, D 55099 Mainz, Germany
    Neuropharmacology 54:141-50. 2008
    ..In addition, it indicates an increased activation of CB1 receptors as a mechanism underlying the effects of FAAH inhibition in two models of anxiety...
  63. ncbi Mutations found in the pncA gene of Mycobacterium tuberculosis in clinical pyrazinamide-resistant isolates from a local region of China
    H Zhang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    J Int Med Res 37:1430-5. 2009
    ..These results show the molecular mechanism of pyrazinamide resistance in China and may also contribute towards the prevention of tuberculosis in China...
  64. ncbi Characterization of the fatty acid amide hydrolase inhibitor cyclohexyl carbamic acid 3'-carbamoyl-biphenyl-3-yl ester (URB597): effects on anandamide and oleoylethanolamide deactivation
    Darren Fegley
    Department of Pharmacology, University of California, Irvine CA 92697 4625, USA
    J Pharmacol Exp Ther 313:352-8. 2005
    ..The results also suggest that an enzyme distinct from FAAH catalyzes OEA hydrolysis in the duodenum, where this lipid substance acts as a local satiety factor...
  65. ncbi Design, synthesis, and structure-activity relationships of alkylcarbamic acid aryl esters, a new class of fatty acid amide hydrolase inhibitors
    Giorgio Tarzia
    Istituto di Chimica Farmaceutica e Tossicologica, Universita degli Studi di Urbino Carlo Bo, Piazza del Rinascimento 6, Italy
    J Med Chem 46:2352-60. 2003
    ..URB524 (N-cyclohexylcarbamic acid biphenyl-3-yl ester, 9g) is the most potent compound of the series (IC(50) = 63 nM) and was therefore selected for further optimization...
  66. ncbi Does ADMA cause endothelial dysfunction?
    J P Cooke
    Division of Cardiovascular Medicine, Falk Cardiovascular Research Center, Stanford University School of Medicine, Stanford, CA 94305 5406, USA
    Arterioscler Thromb Vasc Biol 20:2032-7. 2000
    ..By inhibiting the production of nitric oxide, ADMA may impair blood flow, accelerate atherogenesis, and interfere with angiogenesis. ADMA may be a novel risk factor for vascular disease...
  67. ncbi Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins
    Birgit Eisenhaber
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Republic Austria
    Bioessays 25:367-85. 2003
    ..This structural proposal would explain the paradoxical properties of the GPI lipid anchor signal motif and of PIG-K/GPI8 orthologs without membrane insertion regions in some species...
  68. ncbi Absence of 2-hydroxylated sphingolipids is compatible with normal neural development but causes late-onset axon and myelin sheath degeneration
    Inge Zöller
    Institute of Physiological Chemistry, University of Bonn, 53115 Bonn, Germany
    J Neurosci 28:9741-54. 2008
    ..Because axon degeneration appear to start rather early with respect to myelin degenerations, these lipids might be required for glial support of axon function...
  69. pmc N-acylethanolamine signalling mediates the effect of diet on lifespan in Caenorhabditis elegans
    Mark Lucanic
    Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, California 94945, USA
    Nature 473:226-9. 2011
    ..This demonstrates a role for NAE signalling in ageing and indicates that NAEs represent a signal that coordinates nutrient status with metabolic changes that ultimately determine lifespan...
  70. ncbi The DDAH/ADMA/NOS pathway
    Cam T L Tran
    Centre for Clinical Pharmacology and Therapeutics, BHF Laboratories, Division of Medicine, University College London, 5 University Street, London, WC1E 6JJ, UK
    Atheroscler Suppl 4:33-40. 2003
    ..This review summarises recent advances in the regulation and function of DDAH enzymes and its role in the regulation of nitric oxide generation...
  71. ncbi Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2
    Kazuhiro Hasegawa
    Department of Internal Medicine, Keio University, Shinanomachi, Shinjuku ku, Tokyo, Japan
    Circ Res 101:e2-10. 2007
    ..Collectively, the DDAH2/ADMA pathway can be a novel therapeutic target for vasculopathy in the ADMA or angiotensin II-induced pathophysiological conditions...
  72. pmc Vanin-1-/- mice exhibit a glutathione-mediated tissue resistance to oxidative stress
    C Berruyer
    Centre d Immunologie de Marseille Luminy CNRS INSERM Université de la Méditerranée, 13288 Marseille, France
    Mol Cell Biol 24:7214-24. 2004
    ..Therefore, Vanin/pantetheinase inhibitors could be useful for treatment of damage due to irradiation and pro-oxidant inducers...
  73. ncbi Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state
    Fredrik Lanner
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    Stem Cells 28:191-200. 2010
    ..We conclude that N-sulfated HS is required for FGF4 signaling to maintain ES cells primed for differentiation in a heterogeneous state. Inhibiting this pathway facilitates a more naïve ground state...
  74. ncbi Homocysteine impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine
    M C Stühlinger
    Section of Vascular Medicine, Stanford University, Stanford, California, USA
    Circulation 104:2569-75. 2001
    ..Accordingly, we determined if homocysteine could increase ADMA levels...
  75. ncbi A simple stopped assay for fatty acid amide hydrolase avoiding the use of a chloroform extraction phase
    Linda Boldrup
    Department of Pharmacology and Clinical Neuroscience, Umea University, SE901 87 Umea, Sweden
    J Biochem Biophys Methods 60:171-7. 2004
    ..It is concluded that the acidic charcoal extraction method provides a robust and simple stopped assay for FAAH without the need to use potentially hazardous solvents like chloroform...
  76. pmc Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans
    Lorina G Baker
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA
    Eukaryot Cell 6:855-67. 2007
    ..Our analysis indicates that chitin deacetylases and the chitosan made by them may prove to be excellent antifungal targets...
  77. pmc S-nitrosylation of dimethylarginine dimethylaminohydrolase regulates enzyme activity: further interactions between nitric oxide synthase and dimethylarginine dimethylaminohydrolase
    James Leiper
    Centre for Clinical Pharmacology and Therapeutics, British Heart Foundation Laboratories, University College London, 5 University Street, London WC1N 6JJ, United Kingdom
    Proc Natl Acad Sci U S A 99:13527-32. 2002
    ..We also identify Cys-His-Glu as a nitrosylation motif that is conserved in a family of arginine handling enzymes...
  78. pmc Molecular analysis of the prokaryotic ubiquitin-like protein (Pup) conjugation pathway in Mycobacterium tuberculosis
    Francisca A Cerda-Maira
    New York University School of Medicine, Department of Microbiology, 550 First Avenue, New York, NY 10016, USA
    Mol Microbiol 77:1123-35. 2010
    ..Finally, using structural models and site-directed mutagenesis our data suggest that Dop and PafA are members of the glutamine synthetase fold family of proteins...
  79. pmc Control of protein life-span by N-terminal methionine excision
    Carmela Giglione
    Protein Maturation, Trafficking and Signaling, UPR2355, Centre National de la Recherche Scientifique, Institut des Sciences du Vegetal, Gif sur Yvette, France
    EMBO J 22:13-23. 2003
    ..Thus, plastid NME is a critical mechanism specifically influencing the life-span of photosystem II polypeptides. A general role of NME in modulating the half-life of key subsets of proteins is suggested...
  80. ncbi Antidepressant-like activity of the fatty acid amide hydrolase inhibitor URB597 in a rat model of chronic mild stress
    Marco Bortolato
    Department of Pharmacology, University of California, Irvine, California 92697 4260, USA
    Biol Psychiatry 62:1103-10. 2007
    ....
  81. pmc Dimethylarginine dimethylaminohydrolase I enhances tumour growth and angiogenesis
    V Kostourou
    Department of Biochemistry and Immunology, St George s Hospital Medical School, London SW17 0RE, UK
    Br J Cancer 87:673-80. 2002
    ....
  82. pmc Dual blockade of FAAH and MAGL identifies behavioral processes regulated by endocannabinoid crosstalk in vivo
    Jonathan Z Long
    The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:20270-5. 2009
    ....
  83. ncbi Targeting the endocannabinoid system for the treatment of cancer--a practical view
    Christopher J Fowler
    Department of Pharmacology and Clinical Neuroscience, Umea University, Umea, Sweden
    Curr Top Med Chem 10:814-27. 2010
    ....
  84. ncbi Segregation of two endocannabinoid-hydrolyzing enzymes into pre- and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala
    A I Gulyas
    Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, PO Box 67, H 1450, Hungary
    Eur J Neurosci 20:441-58. 2004
    ..The differential compartmentalization of the two enzymes suggests that anandamide and 2-AG signalling may subserve functional roles that are spatially segregated at least at the stage of metabolism...
  85. pmc Discovery and characterization of a highly selective FAAH inhibitor that reduces inflammatory pain
    Kay Ahn
    Pfizer Global Research and Development, Groton, CT 06340, USA
    Chem Biol 16:411-20. 2009
    ..These data thus designate PF-3845 as a valuable pharmacological tool for in vivo characterization of the endocannabinoid system...
  86. pmc Mechanism of carbamate inactivation of FAAH: implications for the design of covalent inhibitors and in vivo functional probes for enzymes
    Jessica P Alexander
    The Skaggs Institute for Chemical Biology and Departments of Cell Biology and Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA
    Chem Biol 12:1179-87. 2005
    ..The experimental strategy described herein can be used to create in vivo probes for any enzyme susceptible to covalent inhibition...
  87. pmc The endogenous cannabinoid anandamide has effects on motivation and anxiety that are revealed by fatty acid amide hydrolase (FAAH) inhibition
    Maria Scherma
    Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, NIH, DHHS, Baltimore, MD, USA
    Neuropharmacology 54:129-40. 2008
    ..Thus, additive interactions between the effects of anandamide on brain reward processes and on anxiety may account for its aversive effects when intravenously administered during FAAH inhibition with URB597...
  88. ncbi A second fatty acid amide hydrolase with variable distribution among placental mammals
    Binqing Q Wei
    The Skaggs Institute for Chemical Biology and Departments of Cell Biology and Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 281:36569-78. 2006
    ..The apparent loss of the FAAH-2 gene in some lower mammals should be taken into consideration when extrapolating genetic or pharmacological findings on the fatty acid amide signaling system across species...
  89. pmc Activation of the peripheral endocannabinoid system in human obesity
    Stefan Engeli
    Franz Volhard Clinical Research Center, Charite Campus Buch, 13125 Berlin, Germany
    Diabetes 54:2838-43. 2005
    ..Our findings support the presence of a peripheral endocannabinoid system that is upregulated in human obesity...
  90. ncbi Cyclohexylcarbamic acid 3'- or 4'-substituted biphenyl-3-yl esters as fatty acid amide hydrolase inhibitors: synthesis, quantitative structure-activity relationships, and molecular modeling studies
    Marco Mor
    Dipartimento Farmaceutico, Universita degli Studi di Parma, Parco Area delle Scienze 27 A, I 43100 Parma, Italy
    J Med Chem 47:4998-5008. 2004
    ..The structure-activity characterization reported here should help optimize the pharmacodynamic and pharmacokinetic properties of this class of compounds...
  91. ncbi Dimethylarginine dimethylaminohydrolase regulates nitric oxide synthesis: genetic and physiological evidence
    Hayan Dayoub
    Program in Vascular Medicine and Biology, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, Calif 94305 5246, USA
    Circulation 108:3042-7. 2003
    ..This study was designed to determine if increased expression of DDAH could reduce tissue and plasma levels of the NOS inhibitors and thereby increase NO synthesis...
  92. pmc Structure and activity of human mitochondrial peptide deformylase, a novel cancer target
    Sindy Escobar-Alvarez
    Molecular Pharmacology and Chemistry Program, Sloan Kettering Institute, 415 E 68th Street Zuckerman Z1941, New York, NY 10065, USA
    J Mol Biol 387:1211-28. 2009
    ..Despite the lack of true S2' and S3' binding pockets, confirmed through peptide binding modeling, enzyme kinetics suggest a combined contribution from P2'and P3' positions of a formylated peptide substrate to turnover...
  93. ncbi A fluorescent assay suitable for inhibitor screening and vanin tissue quantification
    Benfang H Ruan
    Inflammation and Immunology, Pfizer Biotherapeutics R and D, Cambridge, MA 02140, USA
    Anal Biochem 399:284-92. 2010
    ..Preliminary screening of a library of 1280 pharmaceutically active compounds identified inhibitors with novel chemical scaffolds. This assay will be a powerful tool for target validation and drug lead identification and characterization...
  94. pmc Sequence variation in DDAH1 and DDAH2 genes is strongly and additively associated with serum ADMA concentrations in individuals with type 2 diabetes
    Sotoodeh Abhary
    Department of Ophthalmology, Flinders Medical Centre and Flinders University, Adelaide, South Australia, Australia
    PLoS ONE 5:e9462. 2010
    ..We sought to determine whether serum ADMA levels in type 2 diabetes are influenced by common polymorphisms in the DDAH1 and DDAH2 genes...
  95. pmc Fatty acid amide hydrolase and monoacylglycerol lipase inhibitors produce anti-allodynic effects in mice through distinct cannabinoid receptor mechanisms
    Steven G Kinsey
    Department of Pharmacology and Toxicology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia, USA
    J Pain 11:1420-8. 2010
    ....
  96. pmc Fatty acid amide hydrolase inhibition heightens anandamide signaling without producing reinforcing effects in primates
    Zuzana Justinova
    Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland, USA
    Biol Psychiatry 64:930-7. 2008
    ..To address this question, we assessed the effects of URB597, a selective anandamide deactivation inhibitor, as a reinforcer of drug-seeking and drug-taking behavior in squirrel monkeys...
  97. pmc Individual and additive effects of the CNR1 and FAAH genes on brain response to marijuana cues
    Francesca M Filbey
    The MIND Research Network, Albuquerque, NM 87106, USA
    Neuropsychopharmacology 35:967-75. 2010
    ....
  98. pmc Targeting fatty acid amide hydrolase (FAAH) to treat pain and inflammation
    Joel E Schlosburg
    Department of Pharmacology and Toxicology, Medical College of Virginia Campus, Virginia Commonwealth University, P O Box 980613, Richmond, Virginia 23298 0613, USA
    AAPS J 11:39-44. 2009
    ....
  99. ncbi Synthesis and biological evaluation of new potential inhibitors of N-acylethanolamine hydrolyzing acid amidase
    Carmela Saturnino
    Department of Pharmaceutical Sciences, University of Salerno, via Ponte Don Melillo 84084, Fisciano, SA, Italy
    Bioorg Med Chem Lett 20:1210-3. 2010
    ..Cyclopentylhexadecanoate (13) exhibited the highest inhibitory activity on NAAA (IC(50)=10.0 microM), without inhibiting FAAH up to 50 microM. Compound 13 may become a useful template to design new NAAA inhibitors...
  100. ncbi Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon
    J A Plumbridge
    Institut de Biologie Physico Chimique, Paris, France
    Mol Microbiol 3:505-15. 1989
    ..A second promoter producing nagD-specific transcripts has been mapped just in front of the nagD gene...
  101. ncbi Molecular identification of a functional homologue of the mammalian fatty acid amide hydrolase in Arabidopsis thaliana
    Rhidaya Shrestha
    Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, Texas 76203, USA
    J Biol Chem 278:34990-7. 2003
    ..Collectively these data now provide support at the molecular level for a conserved mechanism between plants and animals for the metabolism of NAEs...

Research Grants68

  1. STRUCTURAL ANALYSIS OF GLYCOCONJUGATE METABOLISM
    Patrick Van Roey; Fiscal Year: 2000
    ..Glycoamidases are amidohydrolases that convert asparagine residue to aspartic acid and remove the intact oligosaccharide...
  2. NOVEL AMIDASES FOR SYNTHETIC ORGANIC CHEMISTRY
    FRANCISCO MORIS VARAS; Fiscal Year: 2000
    b>Amidohydrolases (amidases) catalyze in vivo the hydrolysis of amide bonds to the corresponding amine and carboxylic acid...
  3. KNOCKOUT MODEL FOR CANAVAN DISEASE
    Reuben Matalon; Fiscal Year: 2001
    ..5) The knock-out mouse will be used in future studies for determining the metabolic role of NAA in brain, for experimentation with enzyme therapy and gene therapy. ..
  4. RESPONSE OF PHENYLKETONURIA TO TETRAHYDROBIOPTERIN (BH4)
    Reuben Matalon; Fiscal Year: 2007
    ..Abstract Not Provided ..
  5. Phenylalanine Hydroxylase Deficiency: Response to BH4
    Reuben Matalon; Fiscal Year: 2002
    ..Treatment with BH4 should lead to a better outcome for PKU patients and for maternal PKU. ..
  6. Fostriecin and Related Protein Phosphatase Inhibitors
    Dale Boger; Fiscal Year: 2005
    ....
  7. ADMA: An endogenous inhibitor of ESC differentiation and EC regeneration
    John Cooke; Fiscal Year: 2007
    ..This proposal will provide fundamental insights regarding the use of ESC- derived EC for endothelial regeneration, with specific attention to therapeutic angiogenesis, and its regulation by NOS. [unreadable] [unreadable] [unreadable]..
  8. Stanford Vascular Biology and Medicine Training Grant
    John Cooke; Fiscal Year: 2007
    ..abstract_text> ..
  9. Genetic Determinants of PAD
    John Cooke; Fiscal Year: 2007
    ..Sinai and Stanford University which has extensive expertise in cell and vascular biological studies, integrative physiology, human genomic and genetic studies, epidemiology and clinical trials in PAD. ..
  10. Drug Abuse Related Polymorphism in Fatty Acid Amide Hydrolase
    Benjamin Cravatt; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. Microarray Platform for Profiling Cancer Proteases
    Benjamin Cravatt; Fiscal Year: 2008
    ..These proteases may in turn represent valuable new markers and targets for the diagnosis and treatment of cancer. ..
  12. Signal-transduction of Endothelial nAChR in Angiogenesis
    John Cooke; Fiscal Year: 2008
    ....
  13. CHEMICAL APPROACHES FOR ACTIVITY BASED PROTEOMICS
    Benjamin Cravatt; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  14. Enzymes That Regulate Fatty Acid Amide Function In Vivo
    Benjamin Cravatt; Fiscal Year: 2009
    ..These studies will elucidate the functions of key enzymes that regulate FAA signaling in vivo. These proteins may represent new targets for the treatment of pain, addiction, and other neurological disorders. ..
  15. MANIPULATION OF THE NOS PATHWAY IN ARTERIAL DISEASE
    John Cooke; Fiscal Year: 2004
    ..These studies may lead to a novel and cost-effective strategy for treatment of peripheral arterial disease. ..
  16. STRUCTURE/FUNCTION STUDIES OF FATTY ACID AMIDE HYDROLASE
    Benjamin Cravatt; Fiscal Year: 2004
    ..abstract_text> ..
  17. Cannabinoid Receptors and Cerebrovascular Function
    Cecilia Hillard; Fiscal Year: 2006
    ..Increased understanding of the mediators that are released by activated microglia and their targets may provide new therapeutic approaches to CNS diseases with an inflammatory component. ..
  18. FAAH GENE MUTATIONS: RISK FACTORS IN DRUG USE/ADDICTION
    JACK SIPE; Fiscal Year: 2006
    ..abstract_text> ..
  19. Prenatal Cannabis Effect on the Endocannabinoid System
    VINOD YARAGUDRI; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  20. Brain Endocannabinoids and Chronic Stress
    Cecilia Hillard; Fiscal Year: 2005
    ..The successful completion of these studies will enhance our understanding of the processes involved in the adaptation of the brain to chronic stress. ..
  21. Alkaline Ceramidase and Sphingolipid Signaling
    Cungui Mao; Fiscal Year: 2008
    ..These studies will firmly establish a role for haCER2 in regulating S1P and S1P-mediated biology and will pave the way for novel therapeutic approaches to cancer. [unreadable] [unreadable]..
  22. ENDOCANNABINOID BIOMARKERS OF OBESITY USING INTEGRATED GENOMICS AND METABOLOMICS
    JACK SIPE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. Gender differences in the interactions between endocannabinoids and stress
    Cecilia Hillard; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  24. GENOMIC ENZYMOLOGY: THE CROTONASE SUPERFAMILY
    JOHN GERLT; Fiscal Year: 2003
    ..5) 3-OH Isobutyryl CoA hydrolase (HIBCH) catalyzes hydrolysis of a thioester in valine catabolism. We will study HIBCH so that we can determine whether the reaction proceeds via and enolate anion or an anionic tetrahedral intermediate. ..
  25. NITRIC OXIDE 'NO' AND THE KIDNEY
    Chris Baylis; Fiscal Year: 2003
    ..Finally, she will evaluate the validity of the use of plasma and urinary NOX and will use Electron Spin Resonance (ESR) spectroscopy as an alternative method of determining NO production in vivo. ..
  26. Structural Studies of NAGS
    Dashuang Shi; Fiscal Year: 2006
    ..abstract_text> ..
  27. Role of Endocannabinoid Signaling in Alcoholism
    BALAPAL BASAVARAJAPPA; Fiscal Year: 2007
    ..The better understanding of these mechanisms will yield new drug therapies with potential in treatment of alcoholism. ..
  28. IMCD phosphodiesterase in pregnancy; role in ECFVE
    Chris Baylis; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  29. GENOMIC ENZYMOLOGY: THE ENOLASE SUPERFAMILY
    JOHN GERLT; Fiscal Year: 2006
    ..4) We will test a structural blueprint for functional diversity in the (beta/alpha)7beta-barrel fold by determining whether new functions can be generated by in vitro evolution. ..
  30. Role of Endocannabinoids in the Behavioral Consequences of Social Isolation
    Daniele Piomelli; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  31. Role of oleoylethanolamide in the control of food intake
    Daniele Piomelli; Fiscal Year: 2010
    ....
  32. Oleylethanolamide derivatives as anti-obesity agents
    Daniele Piomelli; Fiscal Year: 2005
    ....
  33. CONTROL OF ENDOCANNABINOID RELEASE IN VIVO
    Daniele Piomelli; Fiscal Year: 2003
    ..Moreover, by uncovering the neurochemical interactions between cocaine and the endogenous cannabinoids, our studies will contribute to the general understanding of substance abuse and neuropsychiatric disorders. ..
  34. Characterization of a novel brain cannabinoid ligand
    Daniele Piomelli; Fiscal Year: 2010
    ..The objective of our research is to understand how these transmitters are produced and eliminated, and discover innovative medicines that target these processes. ..
  35. Synthetic Models of N/S-Ligated Metal Centers in Biology
    David P Goldberg; Fiscal Year: 2010
    ..g. Parkinson's, Alzheimer's, cancer). ..
  36. L-pyrrolysine Chemical and Structural Biology
    Michael Chan; Fiscal Year: 2009
    ..This will include: (1) structural studies of L-pyrrolysine containing proteins, (2) structural studies involved in L-pyrrolysine incorporation into protein, and (3) chemical synthesis of L-pyrrolysine and its analogs. ..
  37. Role of EGFR in Viral Infected Lung Epithelial Cells
    Martha M Monick; Fiscal Year: 2010
    ..Completion of these studies will add to our understanding of mechanisms of RSV infection of airway epithelium and the role of targeted signaling pathways in the subsequent inflammation and life span of infected cells. ..
  38. VULNERABILITY TO NICOTINE ADDICTION
    Rafael Maldonado; Fiscal Year: 2008
    ..abstract_text> ..
  39. Can beta-lactam antibiotics decrease morphine physical dependence?
    SCOTT RAWLS; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  40. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2009
    ..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..
  41. B12 Biosysthesis and Anaerobic Metabolism in Salmonella
    JORGE ESCALANTE; Fiscal Year: 2009
    ..Lastly, we will begin to dissect the biosynthetic pathways for the lower ligand base. One pathway is largely non-enzymatic while the other one is a piecemeal pathway. ..
  42. Endocytic Trafficking Motifs in Syndecan & LDL receptor
    Kevin Jon Williams; Fiscal Year: 2008
    ..These proposed studies will clarify basic mechanisms and functional consequences of these novel endocytic determinants within the LDLr and syndecans, including the role of raft localization during nutrient delivery. ..
  43. PLASMA MEMBRANE CHANGES IN FERTILIZATION
    William Lennarz; Fiscal Year: 2001
    ..This will be carried out in L. pictus, a sea urchin of another genus that does not cross fertilize with S. purpuratus. ..
  44. Biosynthesis of N-acetylaspartate
    Aryan Namboodiri; Fiscal Year: 2005
    ..Based on the above information, biochemical approaches aimed at replenishing the NAA could be developed as a novel therapeutic strategy. ..
  45. B. anthracis Peptidoglycan Deacetylase as a Drug Target
    David Pritchard; Fiscal Year: 2005
    ..anthracis, by inhibiting the deacetylases which specifically render their cell wall peptidoglycans resistant to the action of lysozyme. ..
  46. Anandamide and the Fatty Amide Hydrolase (FAAH)
    DALE DEUTSCH; Fiscal Year: 2005
    ..The long-term objective of the proposed research is to understand the role that FAAH plays in the inactivation of the endocannabinoids. ..
  47. Conference on Chromatin Modification Pathways
    Tony Kouzarides; Fiscal Year: 2005
    ..This will increase the visibility of young investigators and allow postdocs and graduate students to participate fully. ..
  48. VIRAL VECTOR CORE FACILITY
    James Norris; Fiscal Year: 2005
    ..A detailed organizational plan, substantial institutional support, and a long term plan for maintenance ensure the sustained viability of the proposed Viral Vector Core Facility. ..
  49. CHEMISTRY AND BIOLOGY OF MALONDIALDEHYDE DNA ADDUCTS
    LAWRENCE MARNETT; Fiscal Year: 2004
    ..The results of these experiments will define the chemistry and biology of a family of structurally dynamic lesions in the genome derived from an endogenous metabolic product of lipid oxidation. ..
  50. MODULATION OF NEUROTRANSMITTER RELEASE BY CANNABINOIDS
    Andrew Gifford; Fiscal Year: 2002
    ..Furthermore, these experiments will give information on the mode of action of cannabinoid antagonists and could suggest novel therapeutic uses for this new class of compounds. ..
  51. ENHANCING COLLECTIN MEDIATED HOST DEFENSE
    Kevan Hartshorn; Fiscal Year: 2001
    ..These experiments should yield important insights into basic collection biology and demonstrate that enhancement of collectin-mediated pulmonary host defense can be achieved in vivo through rational alteration of wild type collectins. ..
  52. Transmigration of HIV-1-infected cells in NeuroAIDS
    Kevin Jon Williams; Fiscal Year: 2006
    ..In addition, our work may provide a new use for statins to prevent or treat AIDS-related dementia and to deplete or eradicate the CNS as an HIV-1 sanctuary. [unreadable] [unreadable]..
  53. ALPHA-2 ADRENERGIC CONTROL OF COLONIC FUNCTION IN IBS
    Michael Camilleri; Fiscal Year: 2007
    ..abstract_text> ..
  54. Nitric oxide and superoxide effects on the failing heart
    Yingjie Chen; Fiscal Year: 2006
    ..Additional studies will determine whether mRNA and protein expression and activity for eNOS, nNOS, iNOS, CuZn-SOD, Mn-SOD and Ec-SOD are altered in coronary vessels and myocardium by the presence of CHF. ..
  55. MUROPEPTIDE RECYCLING PATHWAY & BETA LACTAMASE INDUCTION
    James Park; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  56. Small-Molecule Probes for Conditional Modulation of the Synapse Proteome
    Stephen Haggarty; Fiscal Year: 2007
    ..In the future chemical biology in general, and chemical genetics in particular, will play an important role in catalyzing new discoveries and potentially insight into function of the nervous system. ..
  57. Defining an Obesity QTL on Chromosome 3q
    Xiaofeng Zhu; Fiscal Year: 2007
    ..The methodologies applied have been used successfully to identify unique sequence variants in linkage peaks and to conduct fine mapping with specific physiologic candidate genes. ..
  58. Towards tuberculosis control: pyrazinamide susceptibility and resistant mechanism
    Patricia Sheen; Fiscal Year: 2007
    ..This application will advance these two areas by providing both a rapid clinical assay as well as an explanation for the mechanism of PZA resistance. [unreadable] [unreadable]..
  59. Beta-Lactamases and DD-Peptidases: Active Site Chemistry
    Rex F Pratt; Fiscal Year: 2010
    ..E Gyp 0-. ... G'O --a tail ... 'Fn "-'a'- .N. >.t (Nn Nn. om' m' "L" O-- Oil 0.0)-7-.o 9,' -_p >'7 ='L ..
  60. Roles of KIM-1 in Renal Injury and Repair
    WON HAN; Fiscal Year: 2007
    ..abstract_text> ..
  61. Beta-Lactamases and DD-Peptidases: Active Site Chemistry
    REX PRATT; Fiscal Year: 2008
    ..These studies will lead to new insight into the chemistry of [unreadable]-lactamase and transpeptidase active sites, and thus to new directions in antibiotic design. ..
  62. A Transdisciplinary Approach to Cannabis Addiction
    JOSEPH SCHACHT; Fiscal Year: 2008
    ..Knowledge of these markers will allow health care providers to create targeted interventions to treat these disorders. [unreadable] [unreadable] [unreadable]..
  63. Sex Differences in Endocannabioid-Mediated Behaviors
    Christian Reich; Fiscal Year: 2008
    ..Fear conditioning will be performed with established classical-conditioning protocols. [unreadable] [unreadable]..
  64. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
    ....
  65. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
    ..abstract_text> ..
  66. H. Pylory factors associated with peptic ulcer disease
    TIMOTHY COVER; Fiscal Year: 2006
    ..pylori factors that help to determine clinical outcome, and ultimately, may lead to advances in the treatment or prevention of H. pylori-associated human diseases. [unreadable] [unreadable]..