proton translocating atpases

Summary

Summary: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.

Top Publications

  1. ncbi Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria
    J P Abrahams
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nature 370:621-8. 1994
  2. ncbi ATP synthase--a marvellous rotary engine of the cell
    M Yoshida
    Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama 226 8503, Japan
    Nat Rev Mol Cell Biol 2:669-77. 2001
  3. pmc Constitutive activation of a plasma membrane H(+)-ATPase prevents abscisic acid-mediated stomatal closure
    Sylvain Merlot
    Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, UPR 2355, Gif sur Yvette, France
    EMBO J 26:3216-26. 2007
  4. ncbi F1-ATPase is a highly efficient molecular motor that rotates with discrete 120 degree steps
    R Yasuda
    Department of Physics, Faculty of Science and Technology, Keio University, Yokohama, Japan
    Cell 93:1117-24. 1998
  5. ncbi Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump
    M Lu
    Departments of Medicine and Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Biol Chem 276:30407-13. 2001
  6. pmc Viscoelastic dynamics of actin filaments coupled to rotary F-ATPase: angular torque profile of the enzyme
    O Panke
    Division of Biophysics, University of Osnabruck, D 49069 Osnabruck, Germany
    Biophys J 81:1220-33. 2001
  7. ncbi Impaired glycosylation and cutis laxa caused by mutations in the vesicular H+-ATPase subunit ATP6V0A2
    Uwe Kornak
    Institute for Medical Genetics, Charité Universitaetsmedizin Berlin and Max Planck Institute for Molecular Genetics, Berlin, Germany
    Nat Genet 40:32-4. 2008
  8. pmc Pause and rotation of F(1)-ATPase during catalysis
    Y Hirono-Hara
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan
    Proc Natl Acad Sci U S A 98:13649-54. 2001
  9. ncbi Proton pump-rich cell secretes acid in skin of zebrafish larvae
    Li Yih Lin
    Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
    Am J Physiol Cell Physiol 290:C371-8. 2006
  10. ncbi Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light
    Kumi Ueno
    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu 4 2 1 Fukuoka, 810 8560 Japan
    Plant Cell Physiol 46:955-63. 2005

Research Grants

  1. RECONSTITUTION OF RENAL ACIDIFICATION MECHANISMS
    DENNIS STONE; Fiscal Year: 2002
  2. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2013
  3. STRUCTURE OF THE VACUOLAR ATPase
    Stephan Wilkens; Fiscal Year: 2013
  4. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2013
  5. Genetics and Genomics of the yidC paralogs in Streptococcus mutans
    SARA MARIE RASER PALMER; Fiscal Year: 2013
  6. Regulation of Muscle ATP Synthase Beta Subunit Metabolism in Obesity
    Christos S Katsanos; Fiscal Year: 2013
  7. Axial Flow Effects in Proximal Tubule
    TONG WANG WANG; Fiscal Year: 2012
  8. MECHANISMS OF POLARIZED SECRETION BY BONE CELLS
    Steven L Teitelbaum; Fiscal Year: 2013
  9. (Pro)renin receptor and renovascular hypertension
    Helmy M Siragy; Fiscal Year: 2013
  10. The plant-like vacuole of Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2013

Detail Information

Publications340 found, 100 shown here

  1. ncbi Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria
    J P Abrahams
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nature 370:621-8. 1994
    ..Interconversion of the states may be achieved by rotation of the alpha 3 beta 3 subassembly relative to an alpha-helical domain of the gamma-subunit...
  2. ncbi ATP synthase--a marvellous rotary engine of the cell
    M Yoshida
    Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama 226 8503, Japan
    Nat Rev Mol Cell Biol 2:669-77. 2001
    ..The mechanisms by which rotation and catalysis are coupled in the working enzyme are now being unravelled on a molecular scale...
  3. pmc Constitutive activation of a plasma membrane H(+)-ATPase prevents abscisic acid-mediated stomatal closure
    Sylvain Merlot
    Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, UPR 2355, Gif sur Yvette, France
    EMBO J 26:3216-26. 2007
    ....
  4. ncbi F1-ATPase is a highly efficient molecular motor that rotates with discrete 120 degree steps
    R Yasuda
    Department of Physics, Faculty of Science and Technology, Keio University, Yokohama, Japan
    Cell 93:1117-24. 1998
    ..The work done in each step is constant over a broad range of imposed load and is close to the free energy of hydrolysis of one ATP molecule...
  5. ncbi Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump
    M Lu
    Departments of Medicine and Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Biol Chem 276:30407-13. 2001
    ..The direct binding interaction between V-ATPase and aldolase may be a new mechanism for the regulation of the V-ATPase and may underlie the proximal tubule acidification defect in hereditary fructose intolerance...
  6. pmc Viscoelastic dynamics of actin filaments coupled to rotary F-ATPase: angular torque profile of the enzyme
    O Panke
    Division of Biophysics, University of Osnabruck, D 49069 Osnabruck, Germany
    Biophys J 81:1220-33. 2001
    ..It is considered as essential, not only for the robust operation of this ubiquitous enzyme under symmetry mismatch, but also for a high turnover rate of the two counteracting and stepping motor/generators...
  7. ncbi Impaired glycosylation and cutis laxa caused by mutations in the vesicular H+-ATPase subunit ATP6V0A2
    Uwe Kornak
    Institute for Medical Genetics, Charité Universitaetsmedizin Berlin and Max Planck Institute for Molecular Genetics, Berlin, Germany
    Nat Genet 40:32-4. 2008
    ..These results indicate that the a2 subunit of the proton pump has an important role in Golgi function...
  8. pmc Pause and rotation of F(1)-ATPase during catalysis
    Y Hirono-Hara
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan
    Proc Natl Acad Sci U S A 98:13649-54. 2001
    ..The pausing position of the gamma subunit deviates from the ATP-waiting position and is most likely the recently found intermediate 90 degrees position...
  9. ncbi Proton pump-rich cell secretes acid in skin of zebrafish larvae
    Li Yih Lin
    Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
    Am J Physiol Cell Physiol 290:C371-8. 2006
    ....
  10. ncbi Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light
    Kumi Ueno
    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu 4 2 1 Fukuoka, 810 8560 Japan
    Plant Cell Physiol 46:955-63. 2005
    ..We determined isogenes of the plasma membrane H+-ATPase and found the expression of all isogenes of functional plasma membrane H+-ATPases (AHA1-11) in guard cell protoplasts...
  11. ncbi Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation
    Kengo Adachi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku ku, Tokyo 169 8555, Japan
    Cell 130:309-21. 2007
    ..This and other results suggest that the affinity for ADP also decreases with rotation, and thus ADP release contributes part of energy for rotation. Together with previous results, the coupling scheme is now basically complete...
  12. pmc Expression of a constitutively activated plasma membrane H+-ATPase alters plant development and increases salt tolerance
    Frédéric Gévaudant
    Unite de Biochimie Physiologique, Institut des Sciences de la Vie, Universite Catholique de Louvain, B 1348 Louvain la Neuve, Belgium
    Plant Physiol 144:1763-76. 2007
    ..The DeltaPMA4 plants also displayed increased salt tolerance during germination and seedling growth, supporting the hypothesis that H(+)-ATPase is involved in salt tolerance...
  13. pmc Catalytic and mechanical cycles in F-ATP synthases. Fourth in the Cycles Review Series
    Peter Dimroth
    Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zürich Hönggerberg, Switzerland
    EMBO Rep 7:276-82. 2006
    ....
  14. ncbi VMA13 encodes a 54-kDa vacuolar H(+)-ATPase subunit required for activity but not assembly of the enzyme complex in Saccharomyces cerevisiae
    M N Ho
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 268:18286-92. 1993
    ..Taken together, these results indicate that VMA13 encodes the 54-kDa subunit of the V-ATPase and that this subunit is essential for activity, but not assembly, of the enzyme complex...
  15. ncbi Modification of plasma membrane and vacuolar H+ -ATPases in response to NaCL and ABA
    Małgorzata Janicka-Russak
    Plant Physiology Department, Institute of Plant Biology, Wrocław University, Kanonia 6 8, 50 328 Wrocław, Poland
    J Plant Physiol 164:295-302. 2007
    ..These data have provided evidence that under salt stress conditions, the role of ABA action due to salinity on the induction of the PM-H(+)-ATPase could be at the level of gene expression...
  16. ncbi ATP synthesis driven by proton transport in F1F0-ATP synthase
    Joachim Weber
    Department of Biochemistry and Biophysics, Box 712, University of Rochester Medical Center, Rochester, NY 14642, USA
    FEBS Lett 545:61-70. 2003
    ..Here we review the most recent work on rapidly developing areas within the field and present proposals for enzymatic and mechanoenzymatic mechanisms...
  17. ncbi Energy transduction in the F1 motor of ATP synthase
    H Wang
    Department of Molecular and Cellular Biology, College of Natural Resources, University of California, Berkeley 94720 3112, USA
    Nature 396:279-82. 1998
    ....
  18. pmc Citrate secretion coupled with the modulation of soybean root tip under aluminum stress. Up-regulation of transcription, translation, and threonine-oriented phosphorylation of plasma membrane H+-ATPase
    Hong Shen
    Lab of Plant Nutritional Genetics and Root Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, People s Republic of China
    Plant Physiol 138:287-96. 2005
    ..Taken together, our results demonstrated that up-regulation of plasma membrane H+-ATPase activity was associated with the secretion of citrate from soybean roots...
  19. ncbi ATP synthase: an electrochemical transducer with rotatory mechanics
    W Junge
    Universitat Osnabruck, Abt Biophysik, Germany
    Trends Biochem Sci 22:420-3. 1997
    ..We discuss how ion flow through the membrane-intrinsic portion, F0, may generate torque and how this might be transmitted between stator and rotor to finally expel spontaneously formed ATP from F1 into water...
  20. pmc Inhibition of blue light-dependent H+ pumping by abscisic acid through hydrogen peroxide-induced dephosphorylation of the plasma membrane H+-ATPase in guard cell protoplasts
    Xiao Zhang
    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka, Japan 810 8560
    Plant Physiol 136:4150-8. 2004
    ..Moreover, there are at least two inhibition sites by ABA in the BL signaling pathway of guard cells...
  21. ncbi Deficiency of the INCL protein Ppt1 results in changes in ectopic F1-ATP synthase and altered cholesterol metabolism
    Annina Lyly
    National Public Health Institute and FIMM, Institute for Molecular Medicine, Biomedicum Helsinki, PO Box 104, Fin 00251 Helsinki, Finland
    Hum Mol Genet 17:1406-17. 2008
    ..These data indicate neuron-specific changes for F(1)-complex in the Ppt1-deficient cells and give clues for a possible link between lipid metabolism and neurodegeneration in INCL...
  22. ncbi Chaperone release and unfolding of substrates in type III secretion
    Yukihiro Akeda
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 437:911-5. 2005
    ..Our results show a similarity between the mechanisms of substrate recognition by type III protein secretion systems and AAA + ATPase disassembly machines...
  23. ncbi The plasma membrane proton pump ATPase: the significance of gene subfamilies
    Miguel Arango
    Unite de Biochimie Physiologique, Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud 2 20, 1348 Louvain la Neuve, Belgium
    Planta 216:355-65. 2003
    ..Finally, what can we learn from experimental approaches that silence specific genes? In this review, we would like to discuss these questions in the light of recent data...
  24. ncbi Cooperative three-step motions in catalytic subunits of F(1)-ATPase correlate with 80 degrees and 40 degrees substep rotations
    Tomoko Masaike
    Department of Physics, Faculty of Science, Gakushuin University, 1 5 1 Mejiro, Toshima ku, Tokyo 171 8588, Japan
    Nat Struct Mol Biol 15:1326-33. 2008
    ..Moreover, a previously undescribed set of beta conformations, open, closed and partially closed, is revealed in the ATP-waiting dwells. The present study thus bridges the gap between the chemical and mechanical steps in F(1)-ATPase...
  25. pmc Visualization of protein compartmentation within the plasma membrane of living yeast cells
    Katerina Malinska
    Universitat Regensburg, Lehrstuhl für Zellbiologie und Pflanzenphysiologie, 93040 Regensburg, Germany
    Mol Biol Cell 14:4427-36. 2003
    ..These domains were shown to be stable in growing cells for >30 min. To our knowledge, this is the first observation of a cell polarization-independent lateral compartmentation in the plasma membrane of a living cell...
  26. ncbi Structure, mechanism and regulation of the clathrin-coated vesicle and yeast vacuolar H(+)-ATPases
    M Forgac
    Department of Physiology, Tufts University School of Medicine, Boston, MA 02111, USA
    J Exp Biol 203:71-80. 2000
    ....
  27. pmc Symbiotic status, phosphate, and sucrose regulate the expression of two plasma membrane H+-ATPase genes from the mycorrhizal fungus Glomus mosseae
    Natalia Requena
    Physiological Ecology of Plants Department, Botanical Institute, University of Tubingen, Auf der Morgenstelle 1, 72076 Tubingen, Germany
    Plant Physiol 132:1540-9. 2003
    ..Our results indicate that different fungal H(+)-ATPases isoforms might be recruited at different developmental stages possibly responding to the different requirements of the life in symbiosis...
  28. ncbi Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation
    Takayuki Nishizaka
    Kansai Advanced Research Center, Protein Biophysics Group, Iwaoka 588 2, Nishi ku, Kobe 651 2492, Japan
    Nat Struct Mol Biol 11:142-8. 2004
    ....
  29. ncbi Regulation of plasma membrane H(+)-ATPase in fungi and plants
    F Portillo
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier, 4, E 28029, Madrid, Spain
    Biochim Biophys Acta 1469:31-42. 2000
    ..This review focuses on recent results on regulation of plasma membrane H+-ATPase obtained by molecular approaches...
  30. ncbi Mitochondrial targets of drug toxicity
    K B Wallace
    Department of Biochemistry and Molecular Biology, University of Minnesota School of Medicine, Duluth 55812, USA
    Annu Rev Pharmacol Toxicol 40:353-88. 2000
    ....
  31. ncbi The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector
    C Bauerle
    Institute of Molecular Biology, University of Oregon, Eugene 97403
    J Biol Chem 268:12749-57. 1993
    ..These results are discussed in the context of a developing model for V-ATPase assembly in yeast...
  32. ncbi Structure, function and regulation of the vacuolar (H+)-ATPase
    T H Stevens
    Institute of Molecular Biology, University of Oregon, Eugene 97403 1229, USA
    Annu Rev Cell Dev Biol 13:779-808. 1997
    ..Recent information concerning targeting and regulation of V-ATPases has also been obtained...
  33. ncbi Blue light inhibits guard cell plasma membrane anion channels in a phototropin-dependent manner
    Holger Marten
    Molecular Plant Physiology and Biophysics, Julius von Sachs Institute for Biosciences, Biocenter, Wurzburg University, Julius von Sachs Platz 2, D 97082 Wurzburg, Germany
    Plant J 50:29-39. 2007
    ..These results show that blue light inhibits plasma membrane anion channels through a pathway involving phototropins, in addition to the stimulation of guard cell plasma membrane H(+)-ATPases...
  34. ncbi VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly
    D D Jackson
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Biol Chem 272:25928-34. 1997
    ..We propose that Vma12p functions directly in the assembly of the V0 subunits into a complex in the ER, and that assembly is required for the stability of the V0 subunits and their transport as a complex out of this compartment...
  35. pmc Blue-light- and phosphorylation-dependent binding of a 14-3-3 protein to phototropins in stomatal guard cells of broad bean
    Toshinori Kinoshita
    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka, Japan 810 8560
    Plant Physiol 133:1453-63. 2003
    ..The binding of a 14-3-3 protein to Vfphot was found in etiolated seedlings and leaves in response to BL, suggesting that this event was common to phototropin-mediated responses...
  36. ncbi F1-ATPase, the C-terminal end of subunit gamma is not required for ATP hydrolysis-driven rotation
    Martin Muller
    Universitat Osnabruck, FB Biologie, Abt Biophysik, Barbarastrasse 11, 49076 Osnabruck, Germany
    J Biol Chem 277:23308-13. 2002
    ..These observations imply that the intactness of the C terminus of subunit gamma provides structural stability and/or routing during assembly of the enzyme, but that it is not required for the rotary action under load, proper...
  37. ncbi Axle-less F1-ATPase rotates in the correct direction
    Shou Furuike
    Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku ku, Tokyo 169 8555, Japan
    Science 319:955-8. 2008
    ..Neither a fixed pivot nor a rigid axle was needed for rotation of F1-ATPase...
  38. ncbi Mutations in the gene encoding B1 subunit of H+-ATPase cause renal tubular acidosis with sensorineural deafness
    F E Karet
    Howard Hughes Medical Institute, Department of Medicine, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut 06510, USA
    Nat Genet 21:84-90. 1999
    ..ATP6B1 is the first member of the H+-ATPase gene family in which mutations are shown to cause human disease...
  39. pmc Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex
    K J Hill
    Institute of Molecular Biology, University of Oregon, Eugene 97403 1229
    Mol Biol Cell 5:1039-50. 1994
    ....
  40. pmc One rotary mechanism for F1-ATPase over ATP concentrations from millimolar down to nanomolar
    Naoyoshi Sakaki
    Department of Functional Molecular Science, The Graduate University for Advanced Studies, Nishigonaka 38, Myodaiji, Okazaki 444 8585, Japan
    Biophys J 88:2047-56. 2005
    ..Below 1 nM ATP, we observed less regular rotations, indicative of the appearance of another reaction scheme...
  41. pmc Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g
    Regina Rabl
    Adolf Butenandt Institute for Physiological Chemistry, Ludwig Maximilians University, 81377 Munich, Germany
    J Cell Biol 185:1047-63. 2009
    ..Fcj1 and Su e/g genetically interact. We propose a model in which the antagonism between Fcj1 and Su e/g locally modulates the F(1)F(O) oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristae tips...
  42. ncbi Trans-complex formation by proteolipid channels in the terminal phase of membrane fusion
    C Peters
    Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, , Germany
    Nature 409:581-8. 2001
    ..V0 trans-complexes may therefore form a continuous, proteolipid-lined channel at the fusion site. We propose that radial expansion of such a protein pore may be a mechanism for intracellular membrane fusion...
  43. pmc Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes
    A L Munn
    Department of Biochemistry, Biozentrum of the University of Basel, Switzerland
    J Cell Biol 127:373-86. 1994
    ..The mutations that result in a defect in alpha-factor uptake are not allelic to any of the genes previously shown to encode endocytic functions...
  44. ncbi Recent advances in our understanding of intercalated cells
    Susan M Wall
    Renal Division, Emory University School of Medicine, 1639 Pierce Drive NE, Atlanta, GA 30322, USA
    Curr Opin Nephrol Hypertens 14:480-4. 2005
    ..This review will summarize newly described novel functions of renal intercalated cells...
  45. ncbi Characterization of dominant lethal mutations in the yeast plasma membrane H+-ATPase gene
    F Portillo
    Departamento de Bioquímica de la Facultad de Medicina de la Universidad Autónoma de Madrid, Spain
    FEBS Lett 402:136-40. 1997
    ..We show that 12 of 25 site-directed mutations previously described are actually dominant lethal alleles. In addition, we show that dominant mutant proteins interfere with transport of wild-type ATPase to the plasma membrane...
  46. ncbi Cytosolic Ca2+ homeostasis is a constitutive function of the V-ATPase in Saccharomyces cerevisiae
    C Forster
    Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 275:38245-53. 2000
    ..This compensation was dependent on calcineurin, and was mediated primarily by Pmc1p...
  47. ncbi Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase
    M N Ho
    Institute of Molecular Biology, University of Oregon, Eugene 97403
    J Biol Chem 268:221-7. 1993
    ..Characterization of the vma5 and vma4 mutants revealed that the 42- and 27-kDa subunits are essential for the assembly of the peripheral membrane portion of the H(+)-ATPase onto the vacuolar membrane...
  48. pmc Alteration of the bioenergetic phenotype of mitochondria is a hallmark of breast, gastric, lung and oesophageal cancer
    Antonio Isidoro
    Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, C S I C U A M, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Biochem J 378:17-20. 2004
    ....
  49. ncbi The molecular mechanism of ATP synthesis by F1F0-ATP synthase
    Alan E Senior
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Box 712, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochim Biophys Acta 1553:188-211. 2002
    ..In this review we summarize current state of knowledge and present a hypothesis for the molecular mechanism of ATP synthesis...
  50. ncbi Oxidative phosphorylation at the fin de siècle
    M Saraste
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 102209, D 69012, Heidelberg, Germany
    Science 283:1488-93. 1999
    ..This work, and new enzymological studies of ATP synthase's unusual catalytic mechanism, are reviewed here...
  51. ncbi Mechanically driven ATP synthesis by F1-ATPase
    Hiroyasu Itoh
    Tsukuba Research Laboratory, Hamamatsu Photonics KK, Joko, Hamamatsu 431 3103, Japan
    Nature 427:465-8. 2004
    ..This shows that a vectorial force (torque) working at one particular point on a protein machine can influence a chemical reaction occurring in physically remote catalytic sites, driving the reaction far from equilibrium...
  52. pmc Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+ -ATPase by preventing interaction with 14-3-3 protein
    Anja T Fuglsang
    Department of Plant Biology, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Cell 19:1617-34. 2007
    ..These results suggest that PKS5 is part of a calcium-signaling pathway mediating PM H+ -ATPase regulation...
  53. ncbi Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase
    R Yasuda
    CREST 'Genetic Programming' Team 13, Teikyo University Biotechnology Center 3F, Nogawa 907, Miyamae-ku, Kawasaki 216-0001, Japan
    Nature 410:898-904. 2001
    ....
  54. ncbi Unique rotary ATP synthase and its biological diversity
    Christoph von Ballmoos
    Institut fur Mikrobiologie, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Annu Rev Biophys 37:43-64. 2008
    ....
  55. pmc Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1 prevents mislocalization of mutant ATPase to the vacuole
    A Chang
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    J Cell Biol 128:39-49. 1995
    ..The identification of AST1 homologues suggests that Ast1 belongs to a novel family of proteins that participates in membrane traffic...
  56. ncbi Bovine coupling factor 6, with just 14.5% shared identity, replaces subunit h in the yeast ATP synthase
    J Velours
    Institut de Biochimie et Genetique Cellulaires du CNRS, Universite Victor Segalen, Bordeaux 2, 1 rue Camille Saint Saens, 33077 Bordeaux, Cedex France
    J Biol Chem 276:8602-7. 2001
    ..Like subunit h, bovine F(6) is shown to interact mainly with subunit 4 (subunit b), a component of the second stalk of the enzyme. These data indicated the subunit h is the yeast homolog of mammalian coupling factor F(6)...
  57. ncbi Cell surface F1Fo ATP synthase: a new paradigm?
    Sulene L Chi
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    Ann Med 38:429-38. 2006
    ..This review addresses the expression, interactions, functions, and consequences of inhibition of cell surface ATP synthase, an enzyme now displaying a shift in paradigm, as well as of location...
  58. ncbi Physical interaction between aldolase and vacuolar H+-ATPase is essential for the assembly and activity of the proton pump
    Ming Lu
    Department of Medicine, University of California School of Medicine, San Francisco, California 94143 0532, USA
    J Biol Chem 282:24495-503. 2007
    ..Taken together, these findings strongly suggest an important role for physical association between aldolase and V-ATPase in the regulation of the proton pump...
  59. ncbi Mitochondrial F1Fo-ATP synthase: the small subunits e and g associate with monomeric complexes to trigger dimerization
    Karina Wagner
    Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
    J Mol Biol 392:855-61. 2009
    ..We conclude that association of Su e and Su g with monomeric F(1)F(o)-ATP synthase represents an initial step of oligomer formation...
  60. ncbi F1-ATPase rotates by an asymmetric, sequential mechanism using all three catalytic subunits
    Takayuki Ariga
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226 8503, Japan
    Nat Struct Mol Biol 14:841-6. 2007
    ....
  61. ncbi Activation of plant plasma membrane H+-ATPase by 14-3-3 proteins is negatively controlled by two phosphorylation sites within the H+-ATPase C-terminal region
    Geoffrey Duby
    Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud, 5 15, 1348 Louvain la Neuve, Belgium
    J Biol Chem 284:4213-21. 2009
    ..The data also show that phosphorylation of the penultimate Thr and 14-3-3 binding each contribute in part to H(+)-ATPase activation...
  62. pmc Tissue-specific splicing regulator Fox-1 induces exon skipping by interfering E complex formation on the downstream intron of human F1gamma gene
    Kazuhiro Fukumura
    Department of Biology, Graduate School of Science, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    Nucleic Acids Res 35:5303-11. 2007
    ..In addition, we located a region of the Fox-1 protein that is required for inducing exon skipping. Taken together, our data show a novel mechanism of how RNA-binding proteins regulate alternative splicing...
  63. ncbi The mechanism of mitochondrial membrane potential retention following release of cytochrome c in apoptotic GT1-7 neural cells
    A C Rego
    Buck Institute for Age Research, Novato, CA 94945, USA
    Cell Death Differ 8:995-1003. 2001
    ..These results indicate that the maintenance of Delta Psi(M) following release of cyt c may be a consequence of ATP synthase reversal and cytoplasmic ATP hydrolysis in STS-treated GT1-7 cells...
  64. pmc The rotor tip inside a bearing of a thermophilic F1-ATPase is dispensable for torque generation
    Mohammad Delawar Hossain
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biophys J 90:4195-203. 2006
    ..For torque generation, though not for rapid catalysis, most of the rotor-stator contacts on the deeper half of the penetrating portion of the gamma-subunit are dispensable...
  65. ncbi Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides
    Ryuta Mizutani
    Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan
    J Mol Biol 316:919-29. 2002
    ..The reaction steps proposed for the acyl shift are driven entirely by protonation and deprotonation, in which proton ingress and egress is balanced within the splicing site...
  66. ncbi Protein splicing: its discovery and structural insight into novel chemical mechanisms
    Yasuhiro Anraku
    Department of Biosciences, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan
    IUBMB Life 57:563-74. 2005
    ....
  67. pmc Effects of inducing expression of cloned genes for the F0 proton channel of the Escherichia coli F1F0 ATPase
    R A Monticello
    Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201
    J Bacteriol 174:3370-6. 1992
    ..Such induction did result in growth inhibition, but there was no correlation between growth inhibition and either increased membrane proton permeability or the presence of functional, reconstitutable F0...
  68. ncbi Localization and regulation of the ATP6V0A4 (a4) vacuolar H+-ATPase subunit defective in an inherited form of distal renal tubular acidosis
    Paul A Stehberger
    Departments of Cellular and Molecular Physiology, Genetics, and Surgery, Yale University School of Medicine, New Haven, Connecticut, USA
    J Am Soc Nephrol 14:3027-38. 2003
    ..The significance of a4 expression in the proximal tubule in the context of distal renal tubular acidosis will require further clarification...
  69. ncbi The alpha3beta3gamma subcomplex of the F1-ATPase from the thermophilic bacillus PS3 with the betaT165S substitution does not entrap inhibitory MgADP in a catalytic site during turnover
    J M Jault
    Department of Chemistry and Biochemistry, School of Medicine, University of California at San Diego, La Jolla, California 92093 0601, USA
    J Biol Chem 271:28818-24. 1996
    ..In contrast, a Lineweaver-Burk plot for the mutant complex is linear in this range of ATP concentration, displaying a Km of 133 microM and a kcat of 360 s-1...
  70. pmc IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in Torenia fournieri
    Juan Zi Wu
    Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, China
    J Exp Bot 59:2529-43. 2008
    ....
  71. pmc Studies of nucleotide binding to the catalytic sites of Escherichia coli betaY331W-F1-ATPase using fluorescence quenching
    Vladimir V Bulygin
    Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA
    Proc Natl Acad Sci U S A 104:4327-31. 2007
    ..Many previous results of fluorescence quenching of introduced tryptophans appear to require reinterpretation. Our findings support a bi-site catalytic mechanism for F(1)-ATPase...
  72. ncbi Involvement of the VDE homing endonuclease and rapamycin in regulation of the Saccharomyces cerevisiae GSH11 gene encoding the high affinity glutathione transporter
    Tsuyoshi Miyake
    Industrial Technology Center of Okayama Prefecture, 5301 Haga, Okayama 701 1296, Japan
    J Biol Chem 278:39632-6. 2003
    ..Both systems need to be operational (inhibition of TOR and sulfur starvation) for full expression of GSH11...
  73. pmc Single molecule energetics of F1-ATPase motor
    Eiro Muneyuki
    Department of Physics, Faculty of Science and Technology, Chuo University, Tokyo, Japan
    Biophys J 92:1806-12. 2007
    ..The results imply that microscopically defined work at a single molecule level cannot be directly compared with macroscopically defined free energy input...
  74. ncbi Ground state structure of F1-ATPase from bovine heart mitochondria at 1.9 A resolution
    Matthew W Bowler
    The Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, UK
    J Biol Chem 282:14238-42. 2007
    ..Thus, the structure with bound azide represents the ADP inhibited state of the enzyme, and the new structure represents a ground state intermediate in the active catalytic cycle of ATP hydrolysis...
  75. ncbi Homing at an extragenic locus mediated by VDE (PI-SceI) in Saccharomyces cerevisiae
    Satoru Nogami
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg FSB 101, 5 1 5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277 8562 Japan
    Yeast 19:773-82. 2002
    ..Insertion of the VRS in a homozygous diploid significantly lowered the spore germination ability, indicating that a template for gene repair at its allelic locus is essential for efficient homing and survival of yeast cells...
  76. ncbi Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae
    F S Gimble
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Nature 357:301-6. 1992
    ..Cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking the endonuclease coding sequence into one that contains it...
  77. ncbi Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation
    S Kawasaki-Nishi
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Biol Chem 276:17941-8. 2001
    ..These results suggest that dissociation of the V-ATPase complex in vivo is controlled both by the cellular environment and by the 100-kDa a-subunit isoform present in the complex...
  78. ncbi The plasma membrane H(+)-ATPase gene family in Arabidopsis: genomic sequence of AHA10 which is expressed primarily in developing seeds
    J F Harper
    Scripps Research Institute, Department of Cell Biology, La Jolla, CA 92037
    Mol Gen Genet 244:572-87. 1994
    ..Our results indicate that one function of this unusually large H(+)-ATPase gene family is to allow for expression of different isoforms in different cell types...
  79. ncbi Gene structure of human mitochondrial ATP synthase gamma-subunit. Tissue specificity produced by alternative RNA splicing
    C Matsuda
    Department of Biochemistry, Jichi Medical School, Tochigi, Japan
    J Biol Chem 268:24950-8. 1993
    ..This is the first report on tissue-specific isoforms generated by alternative splicing in an energy transducing mitochondrial protein...
  80. ncbi Defects in TCIRG1 subunit of the vacuolar proton pump are responsible for a subset of human autosomal recessive osteopetrosis
    A Frattini
    Istituto di Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche, Segrate, Italy
    Nat Genet 25:343-6. 2000
    ..Our data indicate that mutations in TCIRG1 are a frequent cause of autosomal recessive osteopetrosis in humans...
  81. ncbi Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence
    Carmen M Moure
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Biol 9:764-70. 2002
    ..The two overlapping non-identical active sites in the endonucleolytic center contain two Ca(+2) ions that coordinate to the catalytic Asp residues. Structure analysis indicates that the top strand may be cleaved first...
  82. pmc Specific binding of vf14-3-3a isoform to the plasma membrane H+-ATPase in response to blue light and fusicoccin in guard cells of broad bean
    T Emi
    Department of Biology, Faculty of Sciences, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan
    Plant Physiol 125:1115-25. 2001
    ..These results suggest that the 14-3-3 protein that bound to the plasma membrane H(+)-ATPase in vivo is vf14-3-3a and that it may play a key role in the activation of H(+)-ATPase in guard cells...
  83. ncbi Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis
    Totte Niittyla
    Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Mol Cell Proteomics 6:1711-26. 2007
    ..Relative quantification of phosphorylation changes by phosphoproteomics provides a means for identification of fast responses to external stimuli in plants as a basis for further functional characterization...
  84. ncbi The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase
    M F Manolson
    Department of Biological Science, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213
    J Biol Chem 267:14294-303. 1992
    ....
  85. ncbi Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+ -ATPase by combining X-ray crystallography and electron cryomicroscopy
    Christian Ottmann
    Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universitat Tubingen, Auf der Morgenstelle 5, 72076 Tubingen, Germany
    Mol Cell 25:427-40. 2007
    ..Fitting of 14-3-3 and H(+)-ATPase atomic structures into the 3D reconstruction map suggests the spatial arrangement of the holocomplex...
  86. pmc Mutational analysis of yeast vacuolar H(+)-ATPase
    T Noumi
    Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110
    Proc Natl Acad Sci U S A 88:1938-42. 1991
    ..Other amino acids that may function in proton conductance were changed. It was found that glycine residues may replace amino acids with exchangeable protons...
  87. ncbi Biochemical characterization of the yeast vacuolar H(+)-ATPase
    P M Kane
    Institute of Molecular Biology, University of Oregon, Eugene 97403
    J Biol Chem 264:19236-44. 1989
    ..Its structure and subunit composition are very similar to other vacuolar ATPase, and it shares some characteristics with the F1F0-ATPases...
  88. ncbi Phot1 and phot2 mediate blue light regulation of stomatal opening
    T Kinoshita
    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka 810 8560, Japan
    Nature 414:656-60. 2001
    ..These results demonstrate that phot1 and phot2 act redundantly as blue light receptors mediating stomatal opening...
  89. ncbi Muscle-specific exonic splicing silencer for exon exclusion in human ATP synthase gamma-subunit pre-mRNA
    Morisada Hayakawa
    Department of Biochemistry, Jichi Medical School, Minamikawachi Machi, Kawachi gun, Tochigi 329 0498, Japan
    J Biol Chem 277:6974-84. 2002
    ..These results demonstrated that the 5'-AGUUCCA-3' immediately downstream from ESE is a muscle-specific exonic splicing silencer (MS-ESS) responsible for exclusion of exon 9 in vivo and in vitro...
  90. ncbi Molecular and physiological characterisation of a 14-3-3 protein from lily pollen grains regulating the activity of the plasma membrane H+ ATPase during pollen grain germination and tube growth
    H Pertl
    , , Austria
    Planta 213:132-41. 2001
    ....
  91. ncbi In vivo activation of plasma membrane H(+)-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis
    Agustín Hernández
    IACR Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, UK
    Eur J Biochem 269:1006-11. 2002
    ....
  92. pmc Plasma membrane depolarization induced by abscisic acid in Arabidopsis suspension cells involves reduction of proton pumping in addition to anion channel activation, which are both Ca2+ dependent
    Mathias Brault
    Laboratoire d Electrophysiologie des Membranes, EA 3514, Universite Paris 7, 75251 Paris 05, France
    Plant Physiol 135:231-43. 2004
    ..Both processes are however dependent on the [Ca2+]cyt increase induced by ABA since increase in [Ca(2+)](cyt) enhanced anion channels and impaired H(+)-ATPases...
  93. ncbi Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells
    Katerina Malinska
    Universitat Regensburg, Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universitatsstrasse 31, 93040 Regensburg, Germany
    J Cell Sci 117:6031-41. 2004
    ..Finally, two other proteins were localized within the frame of the Can1p/Pma1p plasma-membrane partition. We show that Fur4p (another H+ symporter) and Sur7p (a protein of unknown function) occupy the Can1p subdomain...
  94. ncbi STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p
    M F Manolson
    Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    J Biol Chem 269:14064-74. 1994
    ..The function of these 100-kDa homologues may be to target or regulate other common V-ATPase subunits for two distinct cellular locations...
  95. pmc Correlation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation
    Daichi Okuno
    The Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka 567 0047, Japan
    Proc Natl Acad Sci U S A 105:20722-7. 2008
    ..The former point was again confirmed in experiments where F(1) rotation was inhibited by adenosine-5'-(beta,gamma-imino)-triphosphate and/or azide, the most commonly used inhibitors for the crystallization of F(1)...
  96. ncbi Mutations in the a3 subunit of the vacuolar H(+)-ATPase cause infantile malignant osteopetrosis
    U Kornak
    Zentrum fur Molekulare Neurobiologie Hamburg, Universitat Hamburg, Germany
    Hum Mol Genet 9:2059-63. 2000
    ..Our work shows that mutations in the gene encoding the a3 subunit of the proton pump are a rather common cause of infantile osteopetrosis and suggests that this disease is genetically heterogeneous...
  97. ncbi Acidification of the male reproductive tract by a proton pumping (H+)-ATPase
    S Breton
    Renal Unit, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    Nat Med 2:470-2. 1996
    ..This acidification mechanism is a potential target for novel strategies aimed at modulating the acidification capacity of parts of the male reproductive tract and, therefore, in regulating male fertility...
  98. ncbi Mechanism of proton transport by plant plasma membrane proton ATPases
    M J Buch-Pedersen
    Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, Opg 8, 5 sal, 1871 Frederiksberg, Denmark
    J Plant Res 116:507-15. 2003
    ..A model of proton translocation of AHA2 involving the side chains of amino acids Asp684 and Arg655 is discussed...
  99. pmc In Saccharomyces cerevisiae, ATP2 mRNA sorting to the vicinity of mitochondria is essential for respiratory function
    Antoine Margeot
    Laboratoire de Genetique Moleculaire, UMR CNRS 8541, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    EMBO J 21:6893-904. 2002
    ..This demonstrates that mRNA localization to the vicinity of mitochondria plays a critical role in organelle biogenesis...
  100. ncbi Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis
    Liarisa A Shchepina
    AN Belozersky Institute, MV Lomonosov Moscow State University, 4 Khokhlova str, Bldg, A, Moscow 119992, Russia
    Oncogene 21:8149-57. 2002
    ..A role of oligomycin-sensitive F0 component of H+-ATP-synthase in the TNF-induced PTP opening and apoptosis is suggested...
  101. ncbi Composition and assembly of the yeast vacuolar H(+)-ATPase complex
    L A Graham
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Exp Biol 203:61-70. 2000
    ..Two of these proteins, Vma12p and Vma22p, function together in an assembly complex that interacts directly with nascent V-ATPase subunits...

Research Grants62

  1. RECONSTITUTION OF RENAL ACIDIFICATION MECHANISMS
    DENNIS STONE; Fiscal Year: 2002
    ..granules and other endomembrane compartments, has been shown to be mediated by a new class of proton translocating ATPases, termed vacuolar, or V-type, H+ATPases...
  2. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2013
    ..These properties stem from the way S. mutans inserts proteins into its cytoplasmic membrane. This research will explain how proteins get into and through the membrane of this and other related pathogens. ..
  3. STRUCTURE OF THE VACUOLAR ATPase
    Stephan Wilkens; Fiscal Year: 2013
    ....
  4. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2013
    ..brucei, the targeting mechanism of transmembrane proteins to this organelle, and the roles of pyrophosphate and polyphosphate metabolism in T. brucei growth and development. ..
  5. Genetics and Genomics of the yidC paralogs in Streptococcus mutans
    SARA MARIE RASER PALMER; Fiscal Year: 2013
    ..The data generated will provide essential new information that can be used to design novel and more effective therapies to combat dental caries and other bacterial infections. ..
  6. Regulation of Muscle ATP Synthase Beta Subunit Metabolism in Obesity
    Christos S Katsanos; Fiscal Year: 2013
    ..Further, they will provide scientific knowledge to base lifestyle interventions to improve muscle ATP turnover in obese individuals. ..
  7. Axial Flow Effects in Proximal Tubule
    TONG WANG WANG; Fiscal Year: 2012
    ..Possible benefits include identification of target molecules, which may be blocked or modified in order to modulate sodium reabsorption by the kidney. ..
  8. MECHANISMS OF POLARIZED SECRETION BY BONE CELLS
    Steven L Teitelbaum; Fiscal Year: 2013
    ..Additionally Syt VII regulates OB exocytosis. Hence, our specific aims are to determine the mechanisms by which 1) PKC;signaling promotes OC polarization and 2) synaptotagmin VII controls exocytosis by OCs and OBs. ..
  9. (Pro)renin receptor and renovascular hypertension
    Helmy M Siragy; Fiscal Year: 2013
    ..These studies are expected to identify novel pathophysiologic mechanisms related to PRR and its contribution to renal injury and could lead to development of new therapeutic strategies in treating hypertension-induced renal disease. ..
  10. The plant-like vacuole of Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2013
    ..We think that the PLV plays a central role during the extracellular phase of the parasite not only in resisting environmental stress but also as it prepares itself for invading the next host cell. ..
  11. Noninvasive Study of Cerebral ATP Metabolism, Bioenergetics and Brain Function
    Wei Chen; Fiscal Year: 2013
    ....
  12. BICARBONATE TRANSPORT BY THE MATURING RENAL TUBULE
    George J Schwartz; Fiscal Year: 2013
    ..The results of these studies are likely to change our understanding of acid-base physiology and elicit new mechanisms for heretofore unexplained causes of distal renal tubular acidosis. ..
  13. ASSEMBLY OF MEMBRANES IN HEART AND YEAST MITOCHONDRIA
    ALEXANDER A TZAGOLOFF; Fiscal Year: 2013
    ..A second goal is to continue clarifying the functions of accessory protein factors that are essential for assembly of the ATPase and of the bc1 complex, another important member of the terminal respiratory pathway. ..
  14. Active transmembrane water cycling kinetics: A Cellular Metabolic 1H MR Biomarker
    JAMES ALVIN BALSCHI; Fiscal Year: 2013
    ..For example, acute renal failure involves loss of renal transport activity (and likely, water fluxes). Potentially our method will define the severity of metabolic damage, which will likely correlate with prognosis. ..
  15. The Role of PLEKHM1 in Osteoclast Function
    Haibo Zhao; Fiscal Year: 2010
    ..We plan to determine how this new protein regulates the osteoclast, raising the possibility that we will be able to develop a new drug that prevents bone loss. ..
  16. ASSEMBLY OF MEMBRANES IN HEART AND YEAST MITOCHONDRIA
    Alexander Tzagoloff; Fiscal Year: 2009
    ..Further characterizations of the mutants in our collection will enlarge on this information and thereby improve the yeast model as it applies to human mitochondrial diseases. ..
  17. H+-ATPase B-subunit Dysfunction and Calcium Nephrolithiasis
    Orson W Moe; Fiscal Year: 2010
    ....
  18. TRANSPORT OF NEUROTRANSMITTER INTO SYNAPTIC VESICLES
    Robert H Edwards; Fiscal Year: 2011
    ..The results will have important implications for our understanding of excitatory neurotransmission, behavior and neuropsychiatric disease. ..
  19. Signal Transduction in Intrapulmonary Chemoreceptors
    Steven C Hempleman; Fiscal Year: 2010
    ..The research will also be used to stimulate interest and give hands-on scientific experience to undergraduate and graduate students at Northern Arizona University interested in biomedical research careers. ..
  20. Energy Coupling and Regulation in the ATP Synthase of E. coli
    Thomas M Duncan; Fiscal Year: 2012
    ..This will guide the rational basis for design of small molecules that selectively inhibit bacterial ATP synthases and not the mitochondrial enzyme. ..
  21. GENETICS OF EXTRACELLULAR MATRIX IN HEALTH AND DISEASE
    Zsolt Urban; Fiscal Year: 2013
    ..We will use genetics and small molecule drugs to identify the contribution of EF dysfunction and altered TGFb signaling to developmental lesions. ..
  22. TRAFFICKING PATHWAYS TO THE CELL SURFACE IN YEAST
    Amy Chang; Fiscal Year: 2009
    ....
  23. Gap Junction-mediated Regulation of the V-type H+-ATPase in a Renal Epithelium
    Peter M Piermarini; Fiscal Year: 2012
    ..In aim 2, I will use a functional genetic approach (RNA interference) to test the hypothesis that innexins-the gap-junction proteins of insects-are the molecular entities that couple principal cells to stellate cells. ..
  24. Elucidating RIN4-Mediated Immune Signaling Cascades in Arabidopsis
    GITTA LAUREL COAKER; Fiscal Year: 2013
    ..Because there are significant similarities between innate immune systems in plants and other eukaryotes, we anticipate our findings will be broadly relevant to NIH's mission. ..
  25. TRANSPORT OF NEUROTRANSMITTER INTO SYNAPTIC VESICLES
    Robert H Edwards; Fiscal Year: 2013
    ..abstract_text> ..
  26. An epithelial model for V-type H+-ATPase-driven acid-base transport
    Peter M Piermarini; Fiscal Year: 2010
    ..Moreover, mosquito kidneys are much more accessible and easier to study in the laboratory than mammalian kidneys, making the former an ideal experimental model system for studying the latter. ..
  27. Structure and function of the ATP synthase
    DAVID MICHAEL MUELLER; Fiscal Year: 2013
    ..The high-resolution structure of the ATP synthase will provide the critical features to understand and treat diseases such as cancer and diabetes. ..
  28. OXIDATIVE PHOSPHORYLATION IN CARDIAC HYPERTROPHY
    TERRENCE O BRIEN; Fiscal Year: 2000
    ..Studying the genetic regulation of oxidative phosphorylation in cardiocytes subjected to hypertrophic stimulus should lead to important, physiologically relevant, insights into key initial molecular events. ..
  29. GENETIC AND BIOCHEMICAL STUDIES OF THE YEAST ATPASE
    David Mueller; Fiscal Year: 1999
    ..This project will provide information on the structure and function of the mitochondrial ATP synthase. In addition, this project will expand upon the broad understanding of the mechanism of catalysis. ..
  30. PROTON TRANSLOCATING SECTOR OF E COLI H+ ATPASE
    ROBERT FILLINGAME; Fiscal Year: 2003
    ..The H+ ATP synthase is central to all cellular functions, i.e. it makes the ATP. Abnormalities in the function of this enzyme, or other mitochondrial respiratory enzymes, result in human disease. ..
  31. CHARGE TRANSFER MECHANISMS IN PROTON PUMPS AND SYMPORTS
    Clifford Slayman; Fiscal Year: 1990
    ....
  32. PROTEOMICS OF REGULATED EXOCYTOSIS IN OSTEOCLASTS
    F Ross; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  33. 2004 & 2006 Membrane Transport Proteins Gordon Conference
    JONATHAN JAVITCH; Fiscal Year: 2005
    ..In keeping with the GRC tradition, the interactions that will result, whether from formal or informal discussions, can be expected to be extraordinarily stimulating and productive. ..
  34. H+ ATPASE IN URINARY ACIDIFICATION
    Stephen Gluck; Fiscal Year: 2001
    ..3. Develop an immortalized intercalated cell line using B1 subunit promoter-driven transforming antigen constructs in transgenic mice. ..
  35. CALMODULIN AND CGMP CONTROL OF OSTEOCLASTIC H+ SECRETION
    HARRY BLAIR; Fiscal Year: 2003
    ..This knowledge will be useful in understanding how osteoporosis develops, and may assist in the design of treatments to reduce bone resorption and prevent osteoporosis. ..
  36. ION TRANSPORT IN AIRWAY SMOOTH MUSCLE
    Kerry Rhoden; Fiscal Year: 2000
    ..Specific Aim 5 is to determine the effect of receptor agonists on ASM pHi and to investigate the role that agonist- induced changes in pHi may play in excitation-contraction coupling. ..
  37. RENAL INTERCALATED CELL REGULATION AND DIFFERENTIATION
    Lisa Satlin; Fiscal Year: 1990
    ....
  38. ACTIVE H+ TRANSPORT IN URINARY ACIDIFICATION
    Qais Al Awqati; Fiscal Year: 1993
    ..The studies outlined in this proposal aim at clarifying the cellular and molecular mechanisms that underlie this process...
  39. STRUCTURE/FUNCTION OF THE E COLI H+ ATPASE
    WILLIAM BRUSILOW; Fiscal Year: 2000
    ..The goal of this research, therefore, is to elucidate the pathway of energy coupling in the ATPase between the proton channel and the catalytic site. ..
  40. STRUCTURAL STUDIES ON THE ENERGY TRANSDUCING ATPASE
    William Allison; Fiscal Year: 1999
    ..The latter are continuing experiments supported by the parent grant. ..
  41. Regulation of Renal H+ATPase by Glycolysis
    Stephen Gluck; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  42. SYNTHESIS AND ASSEMBLY OF THE E COLI H+-ATPASE
    WILLIAM BRUSILOW; Fiscal Year: 1992
    ..Last, hybrid subunit will be constructed to determine which parts of individual ATPase subunits, and even which amino acid residues, are involved in energy coupling and in subunit-subunit interactions during assembly...
  43. Function of SNAP-23 in H+-ATPase Trafficking in the IMCD
    Jonathon Schwartz; Fiscal Year: 2003
    ..Further, Syntaxin IA and SNAP-23 trafficking and binding characteristics will be examined. ..
  44. ENDOSOMAL PROTON TRANSPORT IN THE RENAL PROXIMAL TUBULE
    RICHARD GURICH; Fiscal Year: 1993
    ..The results of these studies should provide new information regarding the contribution of endocytosis to renal proximal tubule function including acidification in this segment...
  45. ENERGY COUPLING BY THE YEAST PLASMA MEMBRANE H+_ATPASE
    David Perlin; Fiscal Year: 2004
    ..abstract_text> ..
  46. MECHANISM OF OXIDATIVE PHOSPHORYLATION
    D Sanadi; Fiscal Year: 1990
    ....
  47. STRUCTURE FUNCTION ANALYSIS OF ENERGY TRANSDUCTION
    Michael Douglas; Fiscal Year: 1991
    ..The ATPase complex of mitochondria is the best characterized of the intracellular proton translocating ATPases with regard to its structure and function...
  48. Assembly & Conductance of Fo Sector of E coli H+-ATPase
    WILLIAM BRUSILOW; Fiscal Year: 2004
    ....
  49. 1993 GORDON RESEARCH CONFERENCE ON BIOENERGETICS
    Robert Gennis; Fiscal Year: 1993
    ..This rather modest investment can have a substantial impact by reinforcing the enthusiasm for research among these young scientists...
  50. EXPRESSION OF THE RENAL H+ ATPASE IN METANEPHROGENESIS
    Stephen Gluck; Fiscal Year: 2001
    ....
  51. THE EGF RECEPTOR--STRUCTURE, FUNCTION, AND HOMOLOGY
    Charles Fox; Fiscal Year: 1990
    ..Purified glucocorticoid receptor will be phosphorylated by purified EGF receptor to test for decreased ligand binding activity...
  52. Molecular analysis of the dimeric F1/F0-ATP synthase
    Rosemary Stuart; Fiscal Year: 2006
    ....
  53. ENERGY TRANSDUCTION IN NEUROSPORA PLASMA MEMBRANES
    GENE SCARBOROUGH; Fiscal Year: 2003
    ..We shall then proceed with structure determinations of these two important human membrane proteins. ..
  54. A Skpl-containing Complex Regulating V-ATPase Activity
    Patricia Kane; Fiscal Year: 2005
    ..abstract_text> ..
  55. Septate junctional proteins: Proteomic identification and role in diuresis
    KLAUS BEYENBACH; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  56. Molecular & Cellular Bioenergetics Gordon Conf. 2005
    Patricia Kane; Fiscal Year: 2005
    ..The format of the Gordon Conference is designed to maximize opportunities for discussion and to encourage presentation of the latest results in the field. ..
  57. FASEB SUMMER RESEARCH CONFERENCE: TRANSPORT ATPASES
    ALAN SENIOR; Fiscal Year: 2005
    ..This conference, focussed on transport ATPases but multidisciplinary in nature, is therefore very clearly related to the mission of NIH. ..
  58. SYNTHETIC STUDIES RELATED TO CANCER RESEARCH/TREATMENT
    Paul Wender; Fiscal Year: 2005
    ..Overall, this research program is expected to be of significant value in chemistry, biology, and medicine. ..
  59. CHARACTERIZATION OF E.COLI F1FO-ATP SYNTHASE
    ALAN SENIOR; Fiscal Year: 2004
    ..ATP-driven pumps are very widely distributed in nature, and are involved in many disease states. Work to be done here will consequently have broad impact in biology and medicine. ..
  60. BIOCHEMICAL INVESTIGATION OF P-GLYCOPROTEIN
    ALAN SENIOR; Fiscal Year: 2004
    ..Basic knowledge of this kind will be invaluable in devising ways to disable P-glycoprotein and overcome drug-resistance in patients. ..
  61. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  62. An MMi CellCut Laser Mircrodissection System
    Dennis Brown; Fiscal Year: 2006
    ..access and expertise to the MGH research community will be an important part of our mission and bridges the gap between pure microscopy studies and functional genomics that take advantage [unreadable] [unreadable] [unreadable]..