thymine dna glycosylase

Summary

Summary: An enzyme that removes THYMINE and URACIL bases mispaired with GUANINE through hydrolysis of their N-glycosidic bond. These mispaired nucleotides generally occur through the hydrolytic DEAMINATION of 5-METHYLCYTOSINE to thymine.

Top Publications

  1. pmc Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 286:35334-8. 2011
  2. pmc Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair
    Salvatore Cortellino
    Cancer Biology Program and Epigenetics and Progenitor Cells Keystone Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Cell 146:67-79. 2011
  3. pmc Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation
    Hideharu Hashimoto
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Nucleic Acids Res 40:10203-14. 2012
  4. ncbi Cyclical DNA methylation of a transcriptionally active promoter
    Raphaël Métivier
    Universite de Rennes I, CNRS, UMR 6026 Equipe SPARTE, IFR 140 GFAS, Campus de Beaulieu, 35042 Rennes Cedex, France
    Nature 452:45-50. 2008
  5. pmc Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA
    Liang Zhang
    Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA
    Nat Chem Biol 8:328-30. 2012
  6. ncbi Embryonic lethal phenotype reveals a function of TDG in maintaining epigenetic stability
    Daniel Cortázar
    Department of Biomedicine, Institute of Biochemistry and Genetics, University of Basel, 4048 Basel, Switzerland
    Nature 470:419-23. 2011
  7. ncbi Uracil in DNA--occurrence, consequences and repair
    Hans E Krokan
    Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Oncogene 21:8935-48. 2002
  8. pmc Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 105:8890-5. 2008
  9. ncbi Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein
    Hidehisa Takahashi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:5611-21. 2005
  10. ncbi Structure and function in the uracil-DNA glycosylase superfamily
    L H Pearl
    Section of Structural Biology and CRC DNA Repair Enzyme Group, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, SW3 6JB, London, UK
    Mutat Res 460:165-81. 2000

Scientific Experts

Detail Information

Publications135 found, 100 shown here

  1. pmc Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 286:35334-8. 2011
    b>Thymine DNA glycosylase (TDG) excises T from G·T mispairs and is thought to initiate base excision repair (BER) of deaminated 5-methylcytosine (mC)...
  2. pmc Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair
    Salvatore Cortellino
    Cancer Biology Program and Epigenetics and Progenitor Cells Keystone Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Cell 146:67-79. 2011
    ..Here, we show that either knockout or catalytic inactivation of the DNA repair enzyme thymine DNA glycosylase (TDG) leads to embryonic lethality in mice...
  3. pmc Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation
    Hideharu Hashimoto
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Nucleic Acids Res 40:10203-14. 2012
    The mammalian thymine DNA glycosylase (TDG) is implicated in active DNA demethylation via the base excision repair pathway. TDG excises the mismatched base from G:X mismatches, where X is uracil, thymine or 5-hydroxymethyluracil (5hmU)...
  4. ncbi Cyclical DNA methylation of a transcriptionally active promoter
    Raphaël Métivier
    Universite de Rennes I, CNRS, UMR 6026 Equipe SPARTE, IFR 140 GFAS, Campus de Beaulieu, 35042 Rennes Cedex, France
    Nature 452:45-50. 2008
    ..Cyclical changes in the methylation status of promoter CpGs may thus represent a critical event in transcriptional achievement...
  5. pmc Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA
    Liang Zhang
    Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA
    Nat Chem Biol 8:328-30. 2012
    Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway...
  6. ncbi Embryonic lethal phenotype reveals a function of TDG in maintaining epigenetic stability
    Daniel Cortázar
    Department of Biomedicine, Institute of Biochemistry and Genetics, University of Basel, 4048 Basel, Switzerland
    Nature 470:419-23. 2011
    b>Thymine DNA glycosylase (TDG) is a member of the uracil DNA glycosylase (UDG) superfamily of DNA repair enzymes...
  7. ncbi Uracil in DNA--occurrence, consequences and repair
    Hans E Krokan
    Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Oncogene 21:8935-48. 2002
    ..Studies are now underway to examine whether mice deficient in Ung develop lymphoproliferative malignancies and have a different life span...
  8. pmc Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 105:8890-5. 2008
    ..T mispairs, which are implicated in genetic disease, cancer, and aging. Human thymine DNA glycosylase (hTDG) removes T from G...
  9. ncbi Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein
    Hidehisa Takahashi
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 280:5611-21. 2005
    ..This screening resulted in the isolation of cDNAs encoding the b isoform of thymine DNA glycosylase (TDGb)...
  10. ncbi Structure and function in the uracil-DNA glycosylase superfamily
    L H Pearl
    Section of Structural Biology and CRC DNA Repair Enzyme Group, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, SW3 6JB, London, UK
    Mutat Res 460:165-81. 2000
    ....
  11. pmc Cell cycle regulation as a mechanism for functional separation of the apparently redundant uracil DNA glycosylases TDG and UNG2
    Ulrike Hardeland
    Centre for Biomedicine, DKBW, University of Basel, Mattenstrasse 28, CH 4058 Basel, Switzerland
    Nucleic Acids Res 35:3859-67. 2007
    ..Thus, TDG- and UNG2-dependent base excision repair alternates throughout the cell cycle, and the ubiquitin-proteasome pathway constitutes the underlying regulatory system...
  12. pmc Ten-eleven translocation (Tet) and thymine DNA glycosylase (TDG), components of the demethylation pathway, are direct targets of miRNA-29a
    Pei Zhang
    Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, CT 06520, USA
    Biochem Biophys Res Commun 437:368-73. 2013
    ..5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which can be further oxidized and repaired by thymine DNA glycosylase (TDG), to influence gene transcription in embryonic and adult tissues...
  13. pmc Dependence of substrate binding and catalysis on pH, ionic strength, and temperature for thymine DNA glycosylase: Insights into recognition and processing of G·T mispairs
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, 21201, USA
    DNA Repair (Amst) 10:545-53. 2011
    ..b>Thymine DNA glycosylase (TDG) is one of two mammalian enzymes that can specifically remove thymine from G·T mispairs...
  14. pmc The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates DNA repair enzyme TDG glycosylase
    Xin Guan
    Department of Biochemistry and Molecular Biology and Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Nucleic Acids Res 35:6207-18. 2007
    Human (h) DNA repair enzyme thymine DNA glycosylase (hTDG) is a key DNA glycosylase in the base excision repair (BER) pathway that repairs deaminated cytosines and 5-methyl-cytosines...
  15. pmc Specificity of human thymine DNA glycosylase depends on N-glycosidic bond stability
    Matthew T Bennett
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Am Chem Soc 128:12510-9. 2006
    ..While some DNA glycosylases exhibit narrow specificity, others remove multiple forms of damage. Human thymine DNA glycosylase (hTDG) cleaves thymine from mutagenic G...
  16. pmc Human thymine DNA glycosylase (TDG) and methyl-CpG-binding protein 4 (MBD4) excise thymine glycol (Tg) from a Tg:G mispair
    Jung Hoon Yoon
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Nucleic Acids Res 31:5399-404. 2003
    The repair enzymes thymine DNA glycosylase (TDG) and methyl-CpG-binding protein 4 (MBD4) remove thymines from T:G mismatches resulting from deamination of 5-methylcytosine...
  17. pmc Biochemical and structural characterization of the glycosylase domain of MBD4 bound to thymine and 5-hydroxymethyuracil-containing DNA
    Solange Morera
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette Cedex, F 91198, France
    Nucleic Acids Res 40:9917-26. 2012
    ..The recognition mechanism of flipped-out 5hmU bases in MBD4(cat) active site supports the potential role of MBD4, together with TDG, in maintenance of genome stability and active DNA demethylation in mammals...
  18. pmc Lesion processing by a repair enzyme is severely curtailed by residues needed to prevent aberrant activity on undamaged DNA
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 109:8091-6. 2012
    ..A key player is thymine DNA glycosylase (TDG), which excises thymine from mutagenic G·T mispairs that arise by deamination of 5-methylcytosine (..
  19. ncbi DNA damage and mutations produced by chloroacetaldehyde in a CpG-methylated target gene
    Jun Hyuk Choi
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Mutat Res 568:245-56. 2004
    ..CAA-induced DNA adducts were mapped along the supF gene by using thermostable thymine DNA glycosylase (TDG) in conjunction with ligation-mediated PCR or by a Taq polymerase stop assay...
  20. pmc SUMO-1-dependent allosteric regulation of thymine DNA glycosylase alters subnuclear localization and CBP/p300 recruitment
    Ryan D Mohan
    Department of Physiology and Pharmacology, Siebens Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6G 2V4
    Mol Cell Biol 27:229-43. 2007
    Previous studies have demonstrated that the base excision repair enzyme thymine DNA glycosylase (TDG) mediates recruitment of histone acetyltransferases CREB-binding protein (CBP) and p300 to DNA, suggesting a plausible role for these ..
  21. ncbi Functionality of human thymine DNA glycosylase requires SUMO-regulated changes in protein conformation
    Roland Steinacher
    Centre for Biomedicine, Department of Biological Clinical Sciences, University of Basel, Basel, Switzerland
    Curr Biol 15:616-23. 2005
    ....
  22. pmc 7,8-Dihydro-8-oxoadenine, a highly mutagenic adduct, is repaired by Escherichia coli and human mismatch-specific uracil/thymine-DNA glycosylases
    Ibtissam Talhaoui
    Groupe Réparation de l ADN, Universite Paris Sud, Laboratoire Stabilité Génétique et Oncogenèse CNRS, UMR 8200, Institut de Cancerologie Gustave Roussy, F 94805 Villejuif Cedex, France
    Nucleic Acids Res 41:912-23. 2013
    ..coli, we demonstrate that the excision of 8oxoA from 8oxoA•T and 8oxoA•G has an absolute requirement for TDG and MUG, respectively. The data establish that MUG and TDG can counteract the genotoxic effects of 8oxoA residues in vivo...
  23. pmc Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway
    Seden Grippon
    Division of Molecular Biosciences, Sir Alexander Fleming Building, Chemical Biology Centre, Imperial College London, South Kensington, London, SW7 2AZ, UK
    Nucleic Acids Res 39:2593-603. 2011
    ..It therefore provides a new perspective on abasic site protection and the findings are discussed in the context of downstream lesion processing and enzyme communication in the base excision repair pathway...
  24. pmc Selective excision of 5-carboxylcytosine by a thymine DNA glycosylase mutant
    Hideharu Hashimoto
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Mol Biol 425:971-6. 2013
    The mammalian thymine DNA glycosylase (TDG) excises the mismatched base, uracil, thymine or 5-hydroxymethyluracil (5hmU), as well as removes 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) when paired with a guanine...
  25. pmc Activity and crystal structure of human thymine DNA glycosylase mutant N140A with 5-carboxylcytosine DNA at low pH
    Hideharu Hashimoto
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    DNA Repair (Amst) 12:535-40. 2013
    The mammalian thymine DNA glycosylase (TDG) excises 5-carboxylcytosine (5caC) when paired with a guanine in a CpG sequence, in addition to mismatched bases...
  26. pmc Stoichiometry and affinity for thymine DNA glycosylase binding to specific and nonspecific DNA
    Michael T Morgan
    Department of Biochemistry and Molecular Biology and Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Nucleic Acids Res 39:2319-29. 2011
    ..b>Thymine DNA glycosylase (TDG) removes thymine from these G · T lesions, and follow-on base excision repair yields a G · C pair...
  27. pmc Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer
    Xuehe Xu
    From the Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky 40506
    J Biol Chem 289:8881-90. 2014
    ..While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling...
  28. ncbi The role of the Escherichia coli mug protein in the removal of uracil and 3,N(4)-ethenocytosine from DNA
    E Lutsenko
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    J Biol Chem 274:31034-8. 1999
    ..coli that can remove this mutagenic adduct. Thus, the principal role of Mug in E. coli may be to help repair damage to DNA caused by exogenous chemical agents such as chloroacetaldehyde...
  29. pmc Unique mutational profile associated with a loss of TDG expression in the rectal cancer of a patient with a constitutional PMS2 deficiency
    P Vasovcak
    Department of Biology and Medical Genetics, Charles University 2nd Faculty of Medicine and University Hospital Motol, V Uvalu 84, 15006 Prague 5, Czech Republic
    DNA Repair (Amst) 11:616-23. 2012
    ..This case provides the first in vivo evidence of the key role of TDG in protecting the human genome against the deleterious effects of 5-methylcytosine deamination...
  30. ncbi Repair of deaminated base damage by Schizosaccharomyces pombe thymine DNA glycosylase
    Liang Dong
    Department of Genetics and Biochemistry, South Carolina Experiment Station, Clemson University, Room 219 Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, United States
    DNA Repair (Amst) 7:1962-72. 2008
    ..The relationship among amino acid substitutions, binding affinity and base recognition is discussed...
  31. ncbi Role of mismatch-specific uracil-DNA glycosylase in repair of 3,N4-ethenocytosine in vivo
    Juan Jurado
    Groupe Réparation de l AND, CNRS UMR 8113, LBPA ENS Cachan, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France
    DNA Repair (Amst) 3:1579-90. 2004
    ..The increased spontaneous mutation rate for G.C --> A.T transition in the ung mug double mutant as compared to the single ung mutant suggest that MUG may be a back-up repair enzyme to the classic uracil-DNA glycosylase...
  32. ncbi The enigmatic thymine DNA glycosylase
    Daniel Cortázar
    Centre for Biomedicine, Department of Clinical Biological Research, University of Basel, Basel, Switzerland
    DNA Repair (Amst) 6:489-504. 2007
    When it was first isolated from extracts of HeLa cells in Josef Jiricny's laboratory, the thymine DNA glycosylase (TDG) attracted attention because of its ability to remove thymine, i.e. a normal DNA base, from G.T mispairs...
  33. ncbi Enzymology of repair of etheno-adducts
    Laurent Gros
    Groupe Réparation de l ADN, UMR8113 CNRS, LBPA ENS Cachan, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France
    Mutat Res 531:219-29. 2003
    ....
  34. pmc Uracil-DNA glycosylase in base excision repair and adaptive immunity: species differences between man and mouse
    Berit Doseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7491 Trondheim, Norway
    J Biol Chem 286:16669-80. 2011
    ..Our results reveal significant species differences in genomic uracil processing. These findings should be taken into account when mouse models are used in studies of uracil DNA repair and adaptive immunity...
  35. pmc Coordinating the initial steps of base excision repair. Apurinic/apyrimidinic endonuclease 1 actively stimulates thymine DNA glycosylase by disrupting the product complex
    Megan E Fitzgerald
    Department of Biochemistry and Molecular Biology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 283:32680-90. 2008
    ..A prominent example is thymine DNA glycosylase (TDG), which removes T from G...
  36. ncbi A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase
    P Gallinari
    Istituto di Richerche di Biologia Molecolare P Angeletti, Pomezia, Italy
    Nature 383:735-8. 1996
    ....
  37. pmc Base excision by thymine DNA glycosylase mediates DNA-directed cytotoxicity of 5-fluorouracil
    Christophe Kunz
    Institute of Biochemistry and Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland
    PLoS Biol 7:e91. 2009
    ..In this study, we investigated a possible role of thymine DNA glycosylase (TDG), one of four mammalian uracil DNA glycosylases (UDGs), in the cellular response to 5-FU...
  38. ncbi Crystal structure of thymine DNA glycosylase conjugated to SUMO-1
    Daichi Baba
    Graduate School of Integrated Science, Yokohama City University, Yokohama 230 0045, Japan
    Nature 435:979-82. 2005
    ..During DNA mismatch repair, SUMO conjugation of the uracil/thymine DNA glycosylase TDG promotes the release of TDG from the abasic (AP) site created after base excision, and coordinates ..
  39. pmc T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif
    Marie J Lucey
    Department of Oncology, Imperial College London, Du Cane Road, London, W12 0NN, UK
    Nucleic Acids Res 33:6393-404. 2005
    ..We have shown previously that the base excision repair enzyme thymine DNA glycosylase (TDG) acts as a potent coactivator for estrogen receptor-alpha...
  40. pmc Opposing regulatory roles of phosphorylation and acetylation in DNA mispair processing by thymine DNA glycosylase
    Ryan D Mohan
    Department of Physiology and Pharmacology, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Nucleic Acids Res 38:1135-48. 2010
    CpG dinucleotides are mutational hotspots associated with cancer and genetic diseases. Thymine DNA glycosylase (TDG) plays an integral role in CpG maintenance by excising mispaired thymine and uracil in a CpG context and also ..
  41. pmc Excision of 5-halogenated uracils by human thymine DNA glycosylase. Robust activity for DNA contexts other than CpG
    Michael T Morgan
    Department of Biochemistry and Molecular Biology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 282:27578-86. 2007
    b>Thymine DNA glycosylase (TDG) excises thymine from G.T mispairs and removes a variety of damaged bases (X) with a preference for lesions in a CpG.X context...
  42. ncbi Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells
    S K Mokkapati
    Department of Chemistry, 463 Chemistry Building, Wayne State University, Detroit, MI 48202, USA
    Mol Microbiol 41:1101-11. 2001
    ..Thus, Mug joins a very small coterie of DNA repair enzymes whose principal function is to avoid mutations in stationary-phase cells...
  43. pmc Role of two strictly conserved residues in nucleotide flipping and N-glycosylic bond cleavage by human thymine DNA glycosylase
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 284:36680-8. 2009
    b>Thymine DNA glycosylase (TDG) promotes genomic integrity by excising thymine from mutagenic G.T mismatches arising by deamination of 5-methylcytosine, and follow-on base excision repair enzymes restore a G.C pair...
  44. ncbi Chloroethylnitrosourea-derived ethano cytosine and adenine adducts are substrates for Escherichia coli glycosylases excising analogous etheno adducts
    Anton B Guliaev
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    DNA Repair (Amst) 3:1311-21. 2004
    ..However, also as shown by MD, the stacking interaction between the EC base and Phe 30 in the Mug active site is reduced as compared to the epsilonC base, which could account for the lower EC activity observed in this study...
  45. ncbi The DNA glycosylase T:G mismatch-specific thymine DNA glycosylase represses thyroid transcription factor-1-activated transcription
    C Missero
    Stazione Zoologica A Dohrn, Villa Comunale, 80121 Napoli, Italy
    J Biol Chem 276:33569-75. 2001
    ..for factors that regulate TTF-1 transcriptional activity, we isolated three genes (T:G mismatch-specific thymine DNA glycosylase (TDG), homeodomain-interacting protein kinase 2 (HIPK2), and Ajuba), whose products can interact with TTF-..
  46. pmc Crystal structure of a thwarted mismatch glycosylase DNA repair complex
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT
    EMBO J 18:6599-609. 1999
    ....
  47. ncbi Crystal structure of SUMO-3-modified thymine-DNA glycosylase
    Daichi Baba
    Graduate School of Integrated Science, Yokohama City University, Japan
    J Mol Biol 359:137-47. 2006
    ..Structural comparison with the canonical SBM shows that this SBM-like sequence of TDG retains all of the characteristic interactions of the SBM, indicating sequence diversity in the SBM...
  48. pmc The thymine DNA glycosylase MBD4 represses transcription and is associated with methylated p16(INK4a) and hMLH1 genes
    Emiko Kondo
    Department of Molecular Pathology, Tohoku University School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai, Miyagi 980 8575, Japan
    Mol Cell Biol 25:4388-96. 2005
    ..These results suggest that MBD4 is one of the essential components involved in epigenetic silencing in cancer and its repair activity is necessary for the maintenance of hypermethylated promoters...
  49. pmc The base excision repair enzyme MED1 mediates DNA damage response to antitumor drugs and is associated with mismatch repair system integrity
    Salvatore Cortellino
    Program in Human Genetics, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 100:15071-6. 2003
    ..MED1 defects (much like MMR defects) may impair cell cycle arrest and apoptosis induced by DNA damage...
  50. pmc Thymine DNA glycosylase represses myocardin-induced smooth muscle cell differentiation by competing with serum response factor for myocardin binding
    Jiliang Zhou
    Center for Cardiovascular Sciences, Albany Medical College, Albany, New York 12208, USA
    J Biol Chem 283:35383-92. 2008
    ..From this screen, thymine DNA glycosylase (TDG) was identified as a myocardin-associated protein...
  51. ncbi The thymine-DNA glycosylase regulatory domain: residual structure and DNA binding
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, USR CNRS 3078, Universite de Lille 1, 1 rue du Professeur Calmette, 59021 Lille Cedex, France
    Biochemistry 47:6519-30. 2008
    ..T mismatch processing activity of TDG...
  52. ncbi A convenient spectrometric assay system for intracellular quantitative measurement of DNA glycosylase activity
    Shiying Li
    Jiangsu Center of Hepatobiliary Diseases and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, China
    Acta Biochim Biophys Sin (Shanghai) 42:381-7. 2010
    ..b>Thymine DNA glycosylase and methyl-CpG-binding protein 4 are major DNA glycosylases involved in the mismatch repair progress, and ..
  53. ncbi DNA hypermethylation and partial gene silencing of human thymine- DNA glycosylase in multiple myeloma cell lines
    Benjamin Peng
    School of Dental Science, University of Melbourne, Melbourne, Australia
    Epigenetics 1:138-45. 2006
    ..Hypermethylation of DNA damage repair genes in MM cell lines may provide an explanation for the frequent genomic instability, as well as point mutations, that are encountered in MM...
  54. ncbi Novel activity of Escherichia coli mismatch uracil-DNA glycosylase (Mug) excising 8-(hydroxymethyl)-3,N4-ethenocytosine, a potential product resulting from glycidaldehyde reaction
    Bo Hang
    Donner Laboratory, Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Biochemistry 41:2158-65. 2002
    ..We believe that these findings may have biological and structural implications in defining the role of 8-HM-epsilonC in glycosylase recognition/repair...
  55. pmc Mechanisms of base selection by the Escherichia coli mispaired uracil glycosylase
    Pingfang Liu
    Department of Basic Science, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA
    J Biol Chem 283:8829-36. 2008
    ..The presence of a series of glycosylases that focus on different lesion properties, all coexisting within the same cell, would provide a robust and partially redundant repair system necessary for the maintenance of the genome...
  56. pmc Characterization of Dnmt3b:thymine-DNA glycosylase interaction and stimulation of thymine glycosylase-mediated repair by DNA methyltransferase(s) and RNA
    Michael J Boland
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Mol Biol 379:492-504. 2008
    ..G mismatch repair efficiency upon loss of DNA methyltransferase expression, as well as a requirement for an RNA component for correct T.G mismatch repair...
  57. ncbi Mismatch uracil glycosylase from Escherichia coli: a general mismatch or a specific DNA glycosylase?
    Rory J O'Neill
    Imperial College London, Department of Biological Sciences, Sir Alexander Flemming Building, South Kensington, London SW7 2AZ, United Kingdom
    J Biol Chem 278:20526-32. 2003
    ..glycosylase (MUG) was identified in the Escherichia coli genome as a sequence homolog of the mammalian thymine DNA glycosylase, with activity against uracil in U.G mismatches...
  58. ncbi Crystal structure of the mismatch-specific thymine glycosylase domain of human methyl-CpG-binding protein MBD4
    Wei Zhang
    Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, People s Republic of China
    Biochem Biophys Res Commun 412:425-8. 2011
    ..But the different residues in the N-terminal of domain result in the change of charge distribution on the surface of the protein, which suggest the different roles that may relate some diseases...
  59. pmc Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair
    Ya Qiang Li
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Nucleic Acids Res 35:390-400. 2007
    ..In mammals, two repair enzymes, thymine DNA glycosylase (TDG) and methyl-CpG binding domain 4 (MBD4), have evolved to counteract the mutagenic effect of ..
  60. ncbi Substrate specificity of human thymine-DNA glycosylase on exocyclic cytosine adducts
    Bo Hang
    Department of Genome Stability, Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Chem Biol Interact 165:230-8. 2007
    ..These findings expand the TDG substrate range and aid in understanding the structural requirements for TDG substrate specificity...
  61. ncbi SUMO modification: wrestling with protein conformation
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, UK
    Curr Biol 15:R257-9. 2005
    ....
  62. pmc Origin of endogenous DNA abasic sites in Saccharomyces cerevisiae
    Marie Guillet
    CEA, DSV, Departement de Radiobiologie et Radiopathologie, UMR 217 CNRS CEA Radiobiologie Moléculaire et Cellulaire, F 92265 Fontenay aux Roses, France
    Mol Cell Biol 23:8386-94. 2003
    ..Therefore, this result points to the dUTP pool as an important source of the formation of endogenous AP sites in eukaryotes...
  63. ncbi Substrate recognition by a family of uracil-DNA glycosylases: UNG, MUG, and TDG
    Pingfang Liu
    Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Chem Res Toxicol 15:1001-9. 2002
    ..These results provide new insights into the mechanisms of base selection by DNA repair glycosylases...
  64. ncbi Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, UK
    Nat Struct Biol 5:697-701. 1998
    ....
  65. pmc 5-Methylcytosine DNA glycosylase activity is also present in the human MBD4 (G/T mismatch glycosylase) and in a related avian sequence
    B Zhu
    Friedrich Miescher Institut, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Nucleic Acids Res 28:4157-65. 2000
    ..In situ hybridization with antisense RNA indicate that MBD4 is only located in dividing cells of differentiating embryonic tissues...
  66. ncbi Mismatch dependent uracil/thymine-DNA glycosylases excise exocyclic hydroxyethano and hydroxypropano cytosine adducts
    Ewa Borys-Brzywczy
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 52:149-65. 2005
    ..We hypothesize that the enzyme structure undergoes a pH-induced rearrangement allowing the participation of Lys68 of Mug in catalysis via a hydrogen bond interaction of its epsilon-amino group with N(4) of the cytosine exocyclic adducts...
  67. ncbi Evidence for three thymine DNA glycosylases in human cell extracts: substrate specificities of thymine DNA glycosylase activities
    Sibghat Ullah Lari
    Department of Biological and Medical Research MBC 03, King Faisal Specialist Hospital and Research Center KFSH and RC, Riyadh, Saudi Arabia
    Med Sci Monit 11:BR41-9. 2005
    Purified human thymine DNA glycosylase (TDG) recognizes a G: T mispair in a CpG sequence context more strongly than in any other, in addition to its inactivity toward 2-aminopurine: T or 2,6 diaminopurine: T pairs...
  68. ncbi The crystal structure of mismatch-specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans reveals a novel catalytic residue and broad substrate specificity
    Elin Moe
    Norwegian Structural Biology Centre, University of Tromsø, N 9037 Tromsø, Norway
    J Biol Chem 281:569-77. 2006
    ..We suggest that the novel catalytic residue (Asp-93) has evolved to provide drMUG with broad substrate specificity to increase the DNA repair repertoire of D. radiodurans...
  69. ncbi The main role of human thymine-DNA glycosylase is removal of thymine produced by deamination of 5-methylcytosine and not removal of ethenocytosine
    Mika Abu
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, United Kingdom
    J Biol Chem 278:8739-44. 2003
    ....
  70. pmc Efficient removal of uracil from G.U mispairs by the mismatch-specific thymine DNA glycosylase from HeLa cells
    P Neddermann
    Department of Biochemistry, Istituto di Richerche di Biologia Molecolare P Angeletti, Pomezia, Italy
    Proc Natl Acad Sci U S A 91:1642-6. 1994
    ..The enzyme, a 55-kDa polypeptide, was previously characterized as a mismatch-specific thymine DNA glycosylase and was thought to be responsible solely for the correction (to G.C) of G...
  71. pmc Excision of cytosine and thymine from DNA by mutants of human uracil-DNA glycosylase
    B Kavli
    UNIGEN Center for Molecular Biology, The Norwegian University of Science and Technology, Trondheim, Norway
    EMBO J 15:3442-7. 1996
    ..Three of the four new pyrimidine-DNA glycosylases resulted from single nucleotide substitutions, events that may also happen in vivo...
  72. ncbi Expression and characterization of thymine-DNA glycosylase from Aeropyrum pernix
    Xi peng Liu
    School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China
    Protein Expr Purif 70:1-6. 2010
    ..Together, these data suggest that ApeTDG is a TDG protein with weak UDG activity...
  73. pmc Identification of Escherichia coli mismatch-specific uracil DNA glycosylase as a robust xanthine DNA glycosylase
    Hyun Wook Lee
    Department of Genetics and Biochemistry, South Carolina Experiment Station, Clemson, South Carolina 29634, USA
    J Biol Chem 285:41483-90. 2010
    ..DNA glycosylase (MUG) was identified in the Escherichia coli genome as a sequence homolog of the human thymine DNA glycosylase with activity against mismatched uracil base pairs...
  74. pmc The versatile thymine DNA-glycosylase: a comparative characterization of the human, Drosophila and fission yeast orthologs
    Ulrike Hardeland
    Institute of Molecular Cancer Research, University of Zurich, August Forel Strasse 7, Ch 8008 Zurich, Switzerland
    Nucleic Acids Res 31:2261-71. 2003
    ....
  75. pmc Increased mitochondrial DNA damage and down-regulation of DNA repair enzymes in aged rodent retinal pigment epithelium and choroid
    Ai Ling Wang
    Forsythe Laboratory for the Investigation of the Aging Retina, Department of Ophthalmology, Northwestern University School of Medicine, Chicago, IL
    Mol Vis 14:644-51. 2008
    ....
  76. ncbi Kinetics of the action of thymine DNA glycosylase
    T R Waters
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 273:20007-14. 1998
    The time course of removal of thymine by thymine DNA glycosylase has been measured in vitro. Each molecule of thymine DNA glycosylase removes only one molecule of thymine from DNA containing a G...
  77. ncbi Substrate specificity and sequence preference of G:T mismatch repair: incision at G:T, O6-methylguanine:T, and G:U mispairs in DNA by human cell extracts
    Sibghat Ullah Lari
    Department of Biological and Medical Research MBC 03, King Faisal Specialist Hospital and Research Center KFSH and RC, P O Box 3354, Riyadh 11211, Saudi Arabia
    Biochemistry 41:9248-55. 2002
    ..The parental (A1235) extract, when supplemented with ATP and human thymine DNA glycosylase (TDG), acted proficiently on all three substrates, incising immediately 5' to the mismatched thymine or ..
  78. ncbi Mode of action of trifluorothymidine (TFT) against DNA replication and repair enzymes
    Norihiko Suzuki
    Tokushima Research Center, Taiho Pharmaceutical Co, Ltd, 224 2 Hiraishi Ebisuno, Kawauchi, Tokushima 771 0194, Japan
    Int J Oncol 39:263-70. 2011
    ..There was no detectable excision of the TFT pairing to adenine by uracil DNA glycosylase (UDG), thymine DNA glycosylase (TDG), methyl-CpG binding domain 4 (MBD4) or the fractionated HeLa cell extracts, however, TDG and MBD4 ..
  79. pmc Nucleotide excision repair 3' endonuclease XPG stimulates the activity of base excision repairenzyme thymine glycol DNA glycosylase
    T Bessho
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB 7260, University of North CarolinaSchool of Medicine, Chapel Hill, NC 27599 7260, USA
    Nucleic Acids Res 27:979-83. 1999
    ..These results provide in vitro evidence for a second function of XPG protein in DNA repair and a mechanistic basis for its stimulatory activity on HsNTH protein...
  80. ncbi The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 401:301-4. 1999
    ..Correction of m5CpG x TpG mismatches may instead be initiated by the thymine DNA glycosylase, TDG...
  81. ncbi Increased spontaneous mutation frequency in human cells expressing the phage PBS2-encoded inhibitor of uracil-DNA glycosylase
    E H Radany
    Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, Orange, CA 92868, USA
    Mutat Res 461:41-58. 2000
    ..Further in vitro examination revealed that a thymine DNA glycosylase (TDG) previously shown to mediate Ugi-insensitive excision of uracil bases from DNA was not detected in ..
  82. ncbi Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III
    T P Hilbert
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, New York, New York 10016, USA
    J Biol Chem 272:6733-40. 1997
    ....
  83. ncbi 5-Fluorouracil is efficiently removed from DNA by the base excision and mismatch repair systems
    Franziska Fischer
    Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland
    Gastroenterology 133:1858-68. 2007
    ..Surprisingly, although FU is incorporated also into DNA, little is known about its metabolism in this nucleic acid...
  84. ncbi Structure and activity of a thermostable thymine-DNA glycosylase: evidence for base twisting to remove mismatched normal DNA bases
    Clifford D Mol
    Department of Molecular Biology MB4, Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Mol Biol 315:373-84. 2002
    ....
  85. ncbi A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue
    Masashi Takao
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980 8575, Japan
    J Biol Chem 277:42205-13. 2002
    ..These results suggest that NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression...
  86. pmc Investigation of the mechanisms of DNA binding of the human G/T glycosylase using designed inhibitors
    O D Schärer
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 94:4878-83. 1997
    ..In humans this lesion is repaired by a mismatch-specific thymine DNA glycosylase (TDG or G/T glycosylase), which catalyzes specific excision of the thymine base through N-glycosidic bond ..
  87. ncbi Replication across O6-methylguanine by human DNA polymerase beta in vitro. Insights into the futile cytotoxic repair and mutagenesis of O6-methylguanine
    J Singh
    Nelson Institute of Environmental Medicine, New York University Medical Center, Tuxedo, New York 10987, USA
    J Biol Chem 271:28391-8. 1996
    ..This suggests that pol beta, in concert with the T:G-specific thymine DNA glycosylase, may be intricately involved in the futile cytotoxic repair induced by m6dG...
  88. ncbi Cloning and expression of human G/T mismatch-specific thymine-DNA glycosylase
    P Neddermann
    Department of Biochemistry, Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30, 600, 00040 Pomezia Roma, Italy
    J Biol Chem 271:12767-74. 1996
    ..No common amino acid sequence motifs between the G/T-specific enzyme and other DNA glycosylases could be found, suggesting that TDG belongs to a new class of base-excision repair enzymes...
  89. ncbi DNA methyltransferase 3-like affects promoter methylation of thymine DNA glycosylase independently of DNMT1 and DNMT3B in cancer cells
    Heesun Kim
    Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    Int J Oncol 36:1563-72. 2010
    ..As a validation, we confirmed that thymine DNA glycosylase (TDG), an enzyme involved in the base excision repair of mismatched-DNA, was up-regulated in DNMT3L ..
  90. ncbi Thymine-DNA glycosylase and G to A transition mutations at CpG sites
    T R Waters
    Department of Biochemistry and Molecular Biology, University College London, London, UK
    Mutat Res 462:137-47. 2000
    ..These properties of the enzyme suggest that the inefficiency of the base excision repair pathway that it initiates may be the underlying cause of the prevalence of these mutations...
  91. pmc Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity
    Po Wen Chang
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 108 North Greene Street, Baltimore, MD 21201, USA
    BMC Biochem 10:19. 2009
    ..MthI T/G mismatch DNA glycosylase (A50 and L187 at the corresponding respective positions)...
  92. pmc Initiation of strand incision at G:T and O(6)-methylguanine:T base mismatches in DNA by human cell extracts
    S U Lari
    Department of Biological and Medical Research MBC 03, King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia
    Nucleic Acids Res 29:2409-17. 2001
    ....
  93. pmc Dependence of antibody gene diversification on uracil excision
    Javier M Di Noia
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 QH, UK
    J Exp Med 204:3209-19. 2007
    ....
  94. ncbi Cloning and characterization of a mouse homologue (mNthl1) of Escherichia coli endonuclease III
    A H Sarker
    Institute of Cellular and Molecular Biology, Okayama University Medical School, Okayama 700 8558, Japan
    J Mol Biol 282:761-74. 1998
    ..The mNthl1 gene was shown to lie immediately adjacent to the tuberous sclerosis 2 (Tsc2) gene in a 5'-to-5' orientation by sequence analysis and was assigned to chromosome 17A3 by in situ hybridization...
  95. pmc Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA
    Yu Fei He
    Group of DNA Metabolism, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
    Science 333:1303-7. 2011
    ..These data suggest that oxidation of 5mC by Tet proteins followed by TDG-mediated base excision of 5caC constitutes a pathway for active DNA demethylation...
  96. ncbi Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, United Kingdom
    Cell 92:117-29. 1998
    ..Details of the MUG structure explain its thymine DNA-glycosylase activity and the specificity for G:U/T mispairs, which derives from direct recognition of guanine on the complementary strand...
  97. ncbi Retinoic acid receptors interact physically and functionally with the T:G mismatch-specific thymine-DNA glycosylase
    S Um
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, College de France, BP 163, 67404 Illkirch Cedex, France
    J Biol Chem 273:20728-36. 1998
    ..In vitro, TDG enhanced RXR and RXR/RAR binding to their response elements. These data indicate that TDG is not only a repair enzyme, but could also function in the control of transcription...
  98. ncbi Direct detection of chicken genomic DNA for gender determination by thymine-DNA glycosylase
    N Porat
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Br Poult Sci 52:58-65. 2011
    ..4. Currently, these are laboratory results, but we anticipate that further development will allow this method to be used in non-laboratory settings, where PCR cannot be employed...
  99. ncbi Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription
    Marc Tini
    Gene Expression Laboratory, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 9:265-77. 2002
    ..b>Thymine DNA glycosylase (TDG) initiates repair of G/T and G/U mismatches, commonly associated with CpG islands, by removing ..
  100. pmc A human cancer-associated truncation of MBD4 causes dominant negative impairment of DNA repair in colon cancer cells
    S A Bader
    Department of Pathology, Edinburgh Cancer Research Centre, University of Edinburgh, Crewe Road, Edinburgh EH4 2XR, UK
    Br J Cancer 96:660-6. 2007
    MBD4 binds to methylated DNA and acts as a thymine DNA glycosylase in base excision repair...
  101. ncbi MBD4 deficiency reduces the apoptotic response to DNA-damaging agents in the murine small intestine
    Owen James Sansom
    Cardiff School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK
    Oncogene 22:7130-6. 2003
    ..originally identified through its methyl binding domain, but has more recently been characterized as a thymine DNA glycosylase that interacts with the mismatch repair (MMR) protein MLH1...

Research Grants5

  1. Structure and mechanism of CpG specific DNA glycosylases
    ALEX C DROHAT; Fiscal Year: 2013
    ..Two DNA glycosylases recognize G.T lesions, thymine DNA glycosylase (TDG) and methyl binding domain IV (MBD4)...
  2. Mechanistic Insights into TET-Mediated Demethylation in Leukemia
    Louis C Dore; Fiscal Year: 2013
    ..Additionally, we will identify genome-wide occupancy maps for TET1 and TET2, as well as thymine DNA glycosylase, the enzyme responsible for removing 5fC and 5caC from DNA to allow for replacement with an unmethylated ..
  3. Labeling and sequencing of 5-hmC 5-caC and 5-fC in genomic DNA - Resubmission 0
    Chuan He; Fiscal Year: 2013
    ..Significantly, when paired with a normal G, the 5-fC and 5-caC modification can be excised by the human thymine DNA glycosylase (TDG) without the need for base deamination...
  4. Enzyme Repair of Excoyclic Adducts
    Bo Hang; Fiscal Year: 2005
    ..abstract_text> ..
  5. NOVEL TUMOR SUPPRESSOR GENES IN A TRANSGENIC MOUSE MODEL
    Eric Radany; Fiscal Year: 2002
    ..2. Continue high resolution mapping of Loh5 based on allelic loss in tumors. New, single nucleotide polymorphism markers will be derived for the region of mouse chromosome 4 that contains this locus. ..