deoxyribodipyrimidine photo lyase

Summary

Summary: An enzyme that catalyzes the reactivation by light of UV-irradiated DNA. It breaks two carbon-carbon bonds in pyrimidine dimers in DNA. EC 4.1.99.3.

Top Publications

  1. pmc Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes
    Kenichi Hitomi
    Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560 8531, Japan
    Proc Natl Acad Sci U S A 106:6962-7. 2009
  2. pmc A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity
    Christopher P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 103:17696-700. 2006
  3. ncbi Structure and function of DNA photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB 7260, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Chem Rev 103:2203-37. 2003
  4. ncbi Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes
    Monique M van Oers
    Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
    J Gen Virol 86:2069-80. 2005
  5. ncbi Cryptochrome structure and signal transduction
    Chentao Lin
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Annu Rev Plant Biol 54:469-96. 2003
  6. ncbi Role of two histidines in the (6-4) photolyase reaction
    K Hitomi
    Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
    J Biol Chem 276:10103-9. 2001
  7. ncbi Enzymatic photoreactivation: 50 years and counting
    G B Sancar
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina at Chapel Hill, CB 7260, Chapel Hill, NC 27599 7260, USA
    Mutat Res 451:25-37. 2000
  8. ncbi Cyclobutane pyrimidine dimers are responsible for the vast majority of mutations induced by UVB irradiation in mammalian cells
    Y H You
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 276:44688-94. 2001
  9. ncbi Molecular analysis of zebrafish photolyase/cryptochrome family: two types of cryptochromes present in zebrafish
    Y Kobayashi
    Radiation Biology Center, Kyoto University, Yoshidakonoe cho, Sakyoku, Kyoto 606 8501 Japan
    Genes Cells 5:725-38. 2000
  10. ncbi Reaction mechanism of (6-4) photolyase
    X Zhao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 272:32580-90. 1997

Research Grants

Detail Information

Publications156 found, 100 shown here

  1. pmc Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes
    Kenichi Hitomi
    Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560 8531, Japan
    Proc Natl Acad Sci U S A 106:6962-7. 2009
    ....
  2. pmc A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity
    Christopher P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 103:17696-700. 2006
    ..Here, we show that Cry-DASH proteins from bacterial, plant, and animal sources actually are photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA...
  3. ncbi Structure and function of DNA photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB 7260, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Chem Rev 103:2203-37. 2003
  4. ncbi Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes
    Monique M van Oers
    Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
    J Gen Virol 86:2069-80. 2005
    ..Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses...
  5. ncbi Cryptochrome structure and signal transduction
    Chentao Lin
    Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Annu Rev Plant Biol 54:469-96. 2003
    ..Recent studies suggest that cryptochromes undergo a blue light-dependent phosphorylation that affects the conformation, intermolecular interactions, physiological activities, and protein abundance of the photoreceptors...
  6. ncbi Role of two histidines in the (6-4) photolyase reaction
    K Hitomi
    Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
    J Biol Chem 276:10103-9. 2001
    ..The proton transfer shown by this isotope effect supports the proposed mechanism. The substrate binding and the reaction mechanism are discussed in detail using a molecular model...
  7. ncbi Enzymatic photoreactivation: 50 years and counting
    G B Sancar
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina at Chapel Hill, CB 7260, Chapel Hill, NC 27599 7260, USA
    Mutat Res 451:25-37. 2000
    ..Additionally, genetic and molecular studies of PHR1, the S. cerevisiae gene encoding the apoenzyme of photolyase, have led to the identification of previously unknown damage-responsive transcriptional regulators...
  8. ncbi Cyclobutane pyrimidine dimers are responsible for the vast majority of mutations induced by UVB irradiation in mammalian cells
    Y H You
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 276:44688-94. 2001
    ..We conclude that CPDs are responsible for at least 80% of the UVB-induced mutations in this mammalian cell model...
  9. ncbi Molecular analysis of zebrafish photolyase/cryptochrome family: two types of cryptochromes present in zebrafish
    Y Kobayashi
    Radiation Biology Center, Kyoto University, Yoshidakonoe cho, Sakyoku, Kyoto 606 8501 Japan
    Genes Cells 5:725-38. 2000
    ..Mammalian CRYs act as a potent inhibitor of transcriptional activator whose reactions do not depend on light, but Drosophila CRY functions as a light-dependent suppressor of transcriptional inhibitor...
  10. ncbi Reaction mechanism of (6-4) photolyase
    X Zhao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 272:32580-90. 1997
    ..Taken together, the data are consistent with photoinduced electron transfer from reduced FAD to substrate, in a manner analogous to the cyclobutane pyrimidine dimer photolyase...
  11. ncbi Crystal structure of the T(6-4)C lesion in complex with a (6-4) DNA photolyase and repair of UV-induced (6-4) and Dewar photolesions
    Andreas F Glas
    Department for Chemistry and Biochemistry, Ludwig Maximilians University, Butenandtstr 5 13, 81377 Munich, Germany
    Chemistry 15:10387-96. 2009
    ....
  12. ncbi Electron nuclear double resonance differentiates complementary roles for active site histidines in (6-4) photolyase
    Erik Schleicher
    Fachbereich Physik, Freie Universitat Berlin, Arnimallee 14, 14195 Berlin, Germany
    J Biol Chem 282:4738-47. 2007
    ..5, where the enzymatic repair activity is highest, His(358) is deprotonated, whereas His(354) is protonated. Hence, the latter is likely the proton donor that initiates oxetane formation from the (6-4) photoproduct...
  13. ncbi A novel photoreaction mechanism for the circadian blue light photoreceptor Drosophila cryptochrome
    Alex Berndt
    Max Planck Institute of Molecular Physiology, Department of Structural Biology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    J Biol Chem 282:13011-21. 2007
    ..radical represents the light-activated state that mediates resetting of the Drosophila circadian clock...
  14. ncbi Ultrafast catalytic processes in enzymes
    Dongping Zhong
    Department of Physics, The Ohio State University, Columbus, OH 43210, USA
    Curr Opin Chem Biol 11:174-81. 2007
    ....
  15. ncbi Purification and properties of human blue-light photoreceptor cryptochrome 2
    Sezgin Ozgur
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:2926-32. 2003
    ..These findings reveal new properties of this protein already known to function as a circadian photoreceptor and a light-independent negative transcriptional regulator of the clock genes...
  16. ncbi Cyclobutylpyrimidine dimer base flipping by DNA photolyase
    Kathleen S Christine
    Department of Chemistry, Temple University, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 277:38339-44. 2002
    ..This is consistent with base flipping of the lesion into the protein binding cavity with concomitant destacking of the opposing complementary 2-Ap nucleotide...
  17. ncbi Photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Adv Protein Chem 69:73-100. 2004
  18. ncbi Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair
    Alexandra Mees
    Department of Chemistry and Biochemistry, Butenandt Strasse 5 13, Ludwig Maximilians University, D 81377 Munich, Germany
    Science 306:1789-93. 2004
    ....
  19. pmc Ultrafast dynamics and anionic active states of the flavin cofactor in cryptochrome and photolyase
    Ya Ting Kao
    Department of Physics, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 130:7695-701. 2008
    ..These unique properties of anionic flavins suggest a universal mechanism of electron transfer for the initial functional steps of the photolyase/cryptochrome blue-light photoreceptor family...
  20. ncbi Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from Vibrio cholerae
    Erin N Worthington
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:39143-54. 2003
    ..In addition, VcCry1 exhibits RNA binding activity and co-purifies with an RNA of 60-70 nucleotides in length...
  21. ncbi Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites
    Monique M van Oers
    Laboratory of Virology, Wageningen University, 6709 PD Wageningen, The Netherlands
    Virology 330:460-70. 2004
    ..chalcites larvae and infected Trichoplusia ni High Five cells by RT-PCR and 5' and 3' RACE analysis. The possible role of this gene in the biology of the virus is discussed...
  22. pmc Direct observation of thymine dimer repair in DNA by photolyase
    Ya Ting Kao
    Department of Physics, Ohio State University, 174 West 18th Avenue, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 102:16128-32. 2005
    ..These results show that the photocycle of DNA repair by photolyase is through a radical mechanism and completed on subnanosecond time scale at the dynamic active site, with no net change in the redox state of the flavin cofactor...
  23. pmc A new class of DNA photolyases present in various organisms including aplacental mammals
    A Yasui
    Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    EMBO J 13:6143-51. 1994
    ..Our results suggest a divergence of photolyase genes in early evolution...
  24. pmc Cloning of a marsupial DNA photolyase gene and the lack of related nucleotide sequences in placental mammals
    T Kato
    Radiation Biology Center, Kyoto University, Japan
    Nucleic Acids Res 22:4119-24. 1994
    ..These results strongly suggest that humans, as well as other placental mammals, lack the photolyase gene...
  25. ncbi Regulation of the mammalian circadian clock by cryptochrome
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    J Biol Chem 279:34079-82. 2004
  26. ncbi Electronic structure of (6-4) DNA photoproduct repair involving a non-oxetane pathway
    Tatiana Domratcheva
    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    J Am Chem Soc 131:17793-9. 2009
    ..This repair mechanism is in line with the recent structural data on the (6-4) photolyase from D. melanogaster ...
  27. pmc Ultrafast solvation dynamics at binding and active sites of photolyases
    Chih Wei Chang
    Department of Physics, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 107:2914-9. 2010
    ..Such unusual solvation and synergetic dynamics should be general in function sites of proteins...
  28. pmc Fowlpox virus encodes a novel DNA repair enzyme, CPD-photolyase, that restores infectivity of UV light-damaged virus
    V Srinivasan
    Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61802, USA
    J Virol 75:1681-8. 2001
    ..Although expression of a homologous protein has been predicted for some chordopoxviruses, this report is the first to demonstrate that a poxvirus can utilize light to repair damage to its genome...
  29. ncbi Femtochemistry in enzyme catalysis: DNA photolyase
    Ya Ting Kao
    Department of Physics, Chemistry, and Biochemistry, Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    Cell Biochem Biophys 48:32-44. 2007
    ..These observed synergistic motions in the active site reveal a perfect correlation of structural integrity and dynamical locality to ensure maximum repair efficiency on the ultrafast time scale...
  30. ncbi Characterization of (6-4) photoproduct DNA photolyase
    S T Kim
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599 7260
    J Biol Chem 269:8535-40. 1994
    ..Furthermore, we provide evidence that the (6-4) photoproduct photolyase converts the photoproduct to unmodified bases probably through an oxetane intermediate...
  31. pmc Role of the carbonyl group of the (6-4) photoproduct in the (6-4) photolyase reaction
    Junpei Yamamoto
    Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama, Toyonaka, Osaka 560 8531, Japan
    Biochemistry 48:9306-12. 2009
    ..These results indicate that the carbonyl group of the 3' pyrimidone ring plays an important role in the (6-4) photolyase reaction. On the basis of these results, we discuss the repair mechanism...
  32. pmc Flavin adenine dinucleotide as a chromophore of the Xenopus (6-4)photolyase
    T Todo
    Radiation Biology Center, Kyoto University, Japan
    Nucleic Acids Res 25:764-8. 1997
    ..Here we report isolation of a Xenopus laevis(6-4)photolyase gene and show that the (6-4)photolyase binds non- covalently to stoichiometric amounts of FAD. This is the first indication of FAD as the chromophore of (6-4)photolyase...
  33. ncbi Spectroscopic analysis of the pyrimidine(6-4)pyrimidone photoproduct: insights into the (6-4) photolyase reaction
    Junpei Yamamoto
    Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama, Toyonaka, Osaka 560 8531, Japan
    Org Biomol Chem 7:161-6. 2009
    ..The results of this study have implications not only for the recognition and reaction mechanisms of (6-4) photolyase, but also for the chemical nature of the (6-4) photoproduct...
  34. ncbi Sequence analysis of the complete genome of Trichoplusia ni single nucleopolyhedrovirus and the identification of a baculoviral photolyase gene
    Leslie G Willis
    Pacific Agri Food Research Centre, Agriculture and Agri Food Canada, 4200 Highway 97, Summerland, BC, Canada
    Virology 338:209-26. 2005
    ..In vitro fusion assays demonstrated that the TnSNPV F protein induces membrane fusion and syncytia formation and were compared to syncytia formed by AcMNPV GP64...
  35. ncbi Characteristic structure and environment in FAD cofactor of (6-4) photolyase along function revealed by resonance Raman spectroscopy
    Jiang Li
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Myodaiji, Okazaki, Aichi 444 8787, Japan
    J Phys Chem B 110:16724-32. 2006
    ....
  36. ncbi DNA photolyase homologs are the major UV resistance factors in the cyanobacterium Synechocystis sp. PCC 6803
    W O Ng
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Mol Gen Genet 264:924-30. 2001
    ..Based on these findings, we conclude that enzymatic photoreactivation is the primary process used for repairing UV-induced damage in Synechocystis 6803...
  37. ncbi Photorepair of RNA polymerase arrest and apoptosis after ultraviolet irradiation in normal and XPB deficient rodent cells
    V Chiganças
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de São Paulo 05508 900, SP, Brazil
    Cell Death Differ 9:1099-107. 2002
    ..These results demonstrate that marsupial photolyase has access to CPDs blocking RNA synthesis in vivo, and this may be affected by the presence of a mutated XPB protein...
  38. pmc Enhanced repair of cyclobutane pyrimidine dimers and improved UV resistance in photolyase transgenic mice
    Wouter Schul
    MGC, Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    EMBO J 21:4719-29. 2002
    ..Thus, providing mice with CPD photolyase significantly improves repair and uncovers the biological effects of CPD lesions...
  39. pmc Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest
    Hsin Lung Lo
    Division of Biomedical Sciences, University of California Riverside, Riverside, CA 92521, USA
    BMC Cancer 5:135. 2005
    ..In addition, pTpT, a molecular mimic of CPD was tested in vitro and in vivo for the ability to induce cell death and cell cycle alterations...
  40. ncbi A dot-blot immunoassay for measuring repair of ultraviolet photoproducts
    Shirley McCready
    School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK
    Methods Mol Biol 314:229-38. 2006
    ..The method is useful for measuring repair in total genomic DNA. It is more sensitive than most other methods and is sufficiently sensitive to measure repair of damage induced by doses of 10 J/m2 of UV-C in DNA from mammalian cells...
  41. ncbi Repair of cyclobutyl pyrimidine dimers in human skin: variability among normal humans in nucleotide excision and in photorepair
    Betsy M Sutherland
    Biology Department, Building 463, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Photodermatol Photoimmunol Photomed 18:109-16. 2002
    ..We met a similar problem of dimer induction by a PRL source. We designed and validated a PRL source of sufficient intensity to catalyse PR, but that did not induce CPD, and used it to measure photorepair in human skin...
  42. ncbi Light-driven DNA repair by photolyases
    L O Essen
    Department of Chemistry, Philipps University, Hans Meerwein Strasse, 35032 Marburg, Germany
    Cell Mol Life Sci 63:1266-77. 2006
    ..Apart from these mechanistic aspects, the potential of DNA photolyases for the generation of highly UV-resistant organisms, or for skin cancer prevention by ectopical application is increasingly recognized...
  43. pmc Transcriptome analysis reveals cyclobutane pyrimidine dimers as a major source of UV-induced DNA breaks
    George A Garinis
    Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands
    EMBO J 24:3952-62. 2005
    ..Thus, conversion of unrepaired CPD lesions into DNA breaks during DNA replication may comprise one of the principal instigators of UV-mediated cytotoxicity...
  44. ncbi Powerful skin cancer protection by a CPD-photolyase transgene
    Judith Jans
    Department of Genetics, Medical Genetics Center, Center for Biomedical Genetics, Erasmus University Medical Center, Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 15:105-15. 2005
    ....
  45. ncbi Shining a light on photolyases
    Carlos F M Menck
    Nat Genet 32:338-9. 2002
  46. ncbi In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product
    Maureen E Fitch
    Departments of Medicine and Genetics, Division of Oncology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    J Biol Chem 278:46906-10. 2003
    ..XPC did localize to CPDs when p48 was overexpressed in the same cell, signifying that p48 activates the recruitment of XPC to CPDs and may be the initial recognition factor in the NER pathway...
  47. ncbi Genetic and phylogenetic characterization of the type II cyclobutane pyrimidine dimer photolyases encoded by Leporipoxviruses
    C James Bennett
    Department of Molecular Biology and Genetics, The University of Guelph, Guelph, Ontario, N1G 2W1, Canada
    Virology 315:10-9. 2003
    ..Phylogenetic studies also confirmed the monophyletic origin of poxviruses and suggest an ancient origin for these genes and perhaps poxviruses...
  48. ncbi Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice
    Jun Hidema
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980 8577, Japan
    Plant J 43:57-67. 2005
    ....
  49. ncbi Computational studies of DNA photolyase
    Christopher B Harrison
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 5670, USA
    J Phys Chem A 109:7001-12. 2005
    ..The contributions of this work to the overall understanding of the reaction and its relationship to the available experimental work are highlighted...
  50. ncbi Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy
    Erik Schleicher
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Germany
    FEBS J 272:1855-66. 2005
    ..This study provides the basis for future time-resolved FT-IR studies which are aimed at an elucidation of a detailed molecular picture of the light-driven DNA repair process...
  51. ncbi Light-driven enzymatic catalysis of DNA repair: a review of recent biophysical studies on photolyase
    Stefan Weber
    Institute of Experimental Physics, Free University Berlin, Arnimallee 14, 14195 Berlin, Germany
    Biochim Biophys Acta 1707:1-23. 2005
    ..Complementary to these articles, this review focuses on recent biophysical studies of photoreactivation comprising work performed from the early 1990s until the present...
  52. ncbi Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis
    Alexia Chandor
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, DRDC CB, UMR 5047, Commissariat a l Energie Atomique CNRS Universite Joseph Fourier, 17 Rue des Martyrs 38054, Grenoble Cedex 09, France
    J Biol Chem 281:26922-31. 2006
    ....
  53. pmc Crystallization and preliminary X-ray analysis of cryptochrome 3 from Arabidopsis thaliana
    Richard Pokorny
    Philipps Universitat Marburg, FB Biologie, Karl von Frisch Strasse 8, D 35032 Marburg, Germany
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:935-8. 2005
    ..298, b = 116.782, c = 135.024 A. X-ray diffraction data were collected to 1.9 A resolution using synchrotron radiation. The asymmetric unit comprises a cry3 dimer, the physiological role of which remains to be elucidated...
  54. ncbi Analysis of the role of intraprotein electron transfer in photoreactivation by DNA photolyase in vivo
    I Halil Kavakli
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 43:15103-10. 2004
    ..We conclude that photoreduction by intraprotein electron transfer is not part of the photolyase photocycle under physiological conditions...
  55. ncbi The DNA repair enzyme, CPD-photolyase restores the infectivity of UV-damaged fowlpox virus isolated from infected scabs of chickens
    V Srinivasan
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, University of Illinois, Urbana, IL 61802 6178, USA
    Vet Microbiol 108:215-23. 2005
    ..Furthermore, this particular genetic locus can also be used to insert foreign genes for the development of FWPV recombinant vaccines...
  56. ncbi Photolyases: capturing the light to battle skin cancer
    George A Garinis
    Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands
    Future Oncol 2:191-9. 2006
    ..Here, we discuss the use of photolyase transgenic mice as effective tools to study the adverse effects of UV irradiation...
  57. pmc Electrically monitoring DNA repair by photolyase
    Maria C DeRosa
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 102:10788-92. 2005
    ..These results demonstrate the exquisite sensitivity of DNA electrochemistry to perturbations in base pair stacking and the applicability of this chemistry to probe reactions of proteins with DNA...
  58. ncbi Similarities and differences between cyclobutane pyrimidine dimer photolyase and (6-4) photolyase as revealed by resonance Raman spectroscopy: Electron transfer from the FAD cofactor to ultraviolet-damaged DNA
    Jiang Li
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 281:25551-9. 2006
    ..These Raman spectra led us to conclude that the two photolyases have different electron transfer mechanisms as well as different hydrogen bonding environments, which account for the higher redox potential of CPD photolyase...
  59. ncbi Evidence of powerful substrate electric fields in DNA photolyase: implications for thymidine dimer repair
    A W Macfarlane
    Department of Chemistry, Temple University, Philadelphia, Pennsylvania 19122, USA
    Biochemistry 40:15203-14. 2001
    ....
  60. ncbi Characterization of Arabidopsis photolyase enzymes and analysis of their role in protection from ultraviolet-B radiation
    Wanda M Waterworth
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    J Exp Bot 53:1005-15. 2002
    ....
  61. ncbi Diurnal change of cucumber CPD photolyase gene (CsPHR) expression and its physiological role in growth under UV-B irradiation
    Shinya Takahashi
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7 3 1, Bunkyo ku, 113 0033, Japan
    Plant Cell Physiol 43:342-9. 2002
    ..These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation...
  62. ncbi Functional diversity of the DNA photolyase/blue light receptor family
    T Todo
    Radiation Biology Center, Kyoto University, Japan
    Mutat Res 434:89-97. 1999
  63. ncbi Structures and energetics of base flipping of the thymine dimer depend on DNA sequence
    Lauren L O'Neil
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 5670, USA
    J Phys Chem B 112:4113-22. 2008
    ..25-6.5, 5.25-5.5, 7.25-7.5, and 6.5-6.75 kcal/mol, respectively. These energy differences of up to 2 kcal/mol should be large enough to be detected experimentally using sensitive probes...
  64. ncbi DNA repair mechanism by photolyase: electron transfer path from the photolyase catalytic cofactor FADH(-) to DNA thymine dimer
    D Medvedev
    Department of Chemistry, University of California, Davis, CA 95616, USA
    J Theor Biol 210:237-48. 2001
    ..These calculations confirm the previously made conclusion based on an indirect evidence for such mechanism...
  65. ncbi UVB sensitivity and cyclobutane pyrimidine dimer (CPD) photolyase genotypes in cultivated and wild rice species
    Yutaka Iwamatsu
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577, Japan
    Photochem Photobiol Sci 7:311-20. 2008
    ..Knowledge of the activity of different CPD photolyase genotypes will be useful in developing improved rice cultivars...
  66. ncbi No "End of History" for photolyases
    A Sancar
    Department of Biochemistry and Biophysics, University of Nor Carolina School of Medicine, Chapel Hill, 27599, USA
    Science 272:48-9. 1996
  67. ncbi Photoreactivation of (6-4) photolyase in Dunaliella salina
    Xue Yan Lv
    College of Life Sciences, Sichuan University, Chengdu, Sichuan, China
    FEMS Microbiol Lett 283:42-6. 2008
    ..However, evidence is provided that (6-4) photolyases might be highly conserved among different species, not only in the sequence identity but also in the photorepair mechanism...
  68. pmc Photorepair mutants of Arabidopsis
    C Z Jiang
    Section of Plant Biology, University of California, Davis, CA 95616, USA
    Proc Natl Acad Sci U S A 94:7441-5. 1997
    ..This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system...
  69. pmc Theoretical study of excitation energy transfer in DNA photolyase
    Xuehe Zheng
    Department of Chemistry, Center for Macromolecular Modeling and Materials Design, California State Polytechnic University, 3801 West Temple Avenue, Pomona, California 91768, USA
    J Phys Chem B 112:8724-9. 2008
    ..82, a large increase from the experimentally estimated 0.67. Using Förster's mechanism, we find that the energy transfer occurs with remarkable efficiency, comparable with the experimentally determined value of 98%...
  70. ncbi Similarity among the Drosophila (6-4)photolyase, a human photolyase homolog, and the DNA photolyase-blue-light photoreceptor family
    T Todo
    Radiation Biology Center, Kyoto University, Kyoto, Japan
    Science 272:109-12. 1996
    ..A homolog of the Drosophila (6-4)photolyase gene was also cloned from human cells...
  71. ncbi Biochemical and biological properties of DNA photolyases derived from utraviolet-sensitive rice cultivars
    Ayumi Yamamoto
    Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Genes Genet Syst 82:311-9. 2007
    ..coli are similarly proficient. However, the sensitivity to UV of cultivars differs depending on whether Gln or Arg is at 126. The role of Gln and Arg at 126 for photoreactivation in rice is discussed...
  72. ncbi Preferential inhibition of nucleosome assembly by ultraviolet-induced (6-4)photoproducts
    H Matsumoto
    Department of Anatomy, Nara Medical University, Japan
    Photochem Photobiol 61:459-62. 1995
    ..e. linker-like conformation until completion of the repair processes...
  73. ncbi Crystal structure of DNA photolyase from Escherichia coli
    H W Park
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Science 268:1866-72. 1995
    ....
  74. ncbi The Saccharomyces cerevisiae histone acetyltransferase Gcn5 has a role in the photoreactivation and nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers in the MFA2 gene
    Yumin Teng
    School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK
    J Mol Biol 316:489-99. 2002
    ....
  75. ncbi Active site of Escherichia coli DNA photolyase: mutations at Trp277 alter the selectivity of the enzyme without affecting the quantum yield of photorepair
    Y F Li
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Biochemistry 29:5698-706. 1990
    ....
  76. ncbi Identification and expression of the gene product encoding a CPD photolyase from Dunaliella salina
    Long Cheng
    Key Laboratory of Bio resources and Eco environment, Ministry of Education, College of Life Science, Sichuan University, Sichuan 610064, PR China
    J Photochem Photobiol B 87:137-43. 2007
    ..Real-time PCR analysis indicated that the transcription of PHR2 was induced by UV-C, white light, high salinity, and H(2)O(2)...
  77. ncbi Photoreactivation in a phrB mutant of Escherichia coli K-12: evidence for the role of a second protein in photorepair
    N Dorrell
    School of Pharmacy and Pharmacology, University of Bath, Avon, UK
    Photochem Photobiol 58:831-5. 1993
    ..When the putative phrA gene was cloned into an expression vector and transformed into a phrA phrB mutant of E. coli, a level of photorepair was observed, which could correspond to repair of (6-4) photoproducts...
  78. ncbi Dissection of functional domains in Escherichia coli DNA photolyase by linker-insertion mutagenesis
    K Yamamoto
    Biological Institute, Faculty of Science, Tohoku University, Sendai, Japan
    Mol Gen Genet 232:1-6. 1992
    ....
  79. ncbi Efficient cycloreversion of cis,syn-thymine photodimer by a Zn2+ -1,4,7,10-tetraazacyclododecane complex bearing a lumiflavin and tryptophan by chemical reduction and photoreduction of a lumiflavin unit
    Yasuyuki Yamada
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, 278 8510, Japan
    J Biol Inorg Chem 11:1007-23. 2006
    ..Comparison of the photochemical properties of ZnL3 with those of ZnL4 suggested that a tryptophan unit in ZnL3 contributed to the stabilities of the flavin through intramolecular photoinduced electron transfer...
  80. pmc Photolyase confers resistance to UV light but does not contribute to the symbiotic benefit of bioluminescence in Vibrio fischeri ES114
    Emma L Walker
    Department of Microbiology, University of Georgia, 828 Biological Sciences, Athens, GA 30602, USA
    Appl Environ Microbiol 72:6600-6. 2006
    ....
  81. ncbi Non-mutagenic repair of (6-4)photoproducts by (6-4)photolyase purified from Drosophila melanogaster
    T Todo
    Department of Radiation Biology, Faculty of Medicine, Osaka Univ, Japan
    Mutat Res 385:83-93. 1997
    ..Photoreactivation of (6-4)photoproduct constructed in M13 phage eliminates the replication block and abolishes induced mutagenesis in E. coli cells, suggesting that the (6-4)photolyase repairs the photoproduct to the unmodified form...
  82. ncbi Preliminary characterization of light harvesting in E. coli DNA photolyase
    Allison A Henry
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Chembiochem 5:1088-94. 2004
    ..Thus, this protein, the simplest light-harvesting system known, appears to have evolved a remarkably simple mechanism to tune the photophysical properties of the antenna cofactor appropriately for biological function...
  83. pmc qUVR-10, a major quantitative trait locus for ultraviolet-B resistance in rice, encodes cyclobutane pyrimidine dimer photolyase
    Tadamasa Ueda
    National Institute of Agrobiological Sciences, Kannondai 2 1 2, Tsukuba, Ibaraki 305 8602, Japan
    Genetics 171:1941-50. 2005
    ..Furthermore, we were able to develop a UV-hyperresistant plant by overexpression of the photolyase gene...
  84. ncbi Kinetic stability of the flavin semiquinone in photolyase and cryptochrome-DASH
    Michael J Damiani
    Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
    Biochemistry 48:11399-411. 2009
    ..As is being recognized with other flavoproteins, we expect that kinetic tuning of the rates of electron transfer will play a function-defining role in photolyases and cryptochromes...
  85. pmc Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems
    Cristina Bongiorni
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 187:4353-61. 2005
    ..Since the transcription of the RapC- and RapF-encoding genes is positively regulated by their own target ComA, an autoregulatory circuit must exist for the competence transcription factor in order to modulate its activity...
  86. pmc Photoreactivation in phr mutants of Escherichia coli K-12
    I Husain
    J Bacteriol 169:2367-72. 1987
    ....
  87. ncbi On the plausibility of a UV transparent biochemistry
    Charles S Cockell
    British Antarctic Survey, Cambridge, UK
    Orig Life Evol Biosph 32:255-74. 2002
    ..It offers a solution to the long-standing paradox of why the macromolecule responsible for the faithful replication of information has high absorbance in the damaging UV radiation region of the spectrum...
  88. pmc Detecting ultraviolet damage in single DNA molecules by atomic force microscopy
    Yong Jiang
    Center for Biologically Inspired Materials and Material Systems and Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA
    Biophys J 93:1758-67. 2007
    ..Our results suggest that AFM can supplement traditional methods for high resolution measurements of UV damage to DNA...
  89. ncbi Chromatin remodeling activities act on UV-damaged nucleosomes and modulate DNA damage accessibility to photolyase
    Hélène Gaillard
    Institut für Zellbiologie and Institut für Molekularbiologie, Departement Biologie, ETH Honggerberg, CH 8093 Zurich, Switzerland
    J Biol Chem 278:17655-63. 2003
    ..Similar activities might relieve the inhibitory effect of nucleosomes on DNA repair processes in living cells...
  90. ncbi Repair of UV damage in Halobacterium salinarum
    S McCready
    School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK
    Biochem Soc Trans 31:694-8. 2003
    ..However, there are also homologues of eukaryotic nucleotide-excision-repair genes ( Saccharomyces cerevisiae RAD3, RAD25 and RAD2 ) so there may be multiple repair mechanisms for UV damage in Halobacterium...
  91. pmc Discrimination of class I cyclobutane pyrimidine dimer photolyase from blue light photoreceptors by single methionine residue
    Yuji Miyazawa
    Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Biophys J 94:2194-203. 2008
    ..Consistent findings from different disciplines of computational biology strongly suggest the pivotal role of Met-353 in the biological function of DNA photolyase...
  92. ncbi The electronic structure of the flavin cofactor in DNA photolyase
    S Weber
    Institute of Experimental Physics, Free University Berlin, Arnimallee 14, 14195 Berlin, Germany
    J Am Chem Soc 123:3790-8. 2001
    ....
  93. ncbi Towards elucidation of the mechanism of UV1C, a deoxyribozyme with photolyase activity
    Daniel J F Chinnapen
    Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia Canada V5A 1S6
    J Mol Biol 365:1326-36. 2007
    ..Some surprising similarities with bacterial CPD photolyase enzymes are noted...
  94. pmc Construction and analysis of photolyase mutants of Pseudomonas aeruginosa and Pseudomonas syringae: contribution of photoreactivation, nucleotide excision repair, and mutagenic DNA repair to cell survival and mutability following exposure to UV-B radiatio
    J J Kim
    Department of Plant Pathology and Microbiology, Texas A and M University, College Station, TX 77843-2132, USA
    Appl Environ Microbiol 67:1405-11. 2001
    ..The construction and characterization of phr mutants in the present study will facilitate the determination of the roles of light and dark repair systems in organisms exposed to solar radiation in their natural habitats...
  95. ncbi Molecular responses to changes in the environmental pH are conserved between the fungal pathogens Candida dubliniensis and Candida albicans
    W J Heinz
    , , Germany
    Int J Med Microbiol 290:231-8. 2000
    ..In conclusion, C. dubliniensis and C. albicans respond to changes in the environmental pH with a change in cell shape and differential gene expression...
  96. ncbi Resonance Raman spectroscopy of the neutral radical Trp306 in DNA photolyase
    Ullas Gurudas
    Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY 10003, USA
    J Am Chem Soc 126:12788-9. 2004
    ..This important new result demonstrates the potential of time-resolved resonance Raman spectroscopy as a powerful tool to investigate these radicals in protein electron-transfer processes and in enzymatic reactions in real time...
  97. ncbi Effect of xenogenic repair enzymes on photoimmunology and photocarcinogenesis
    H Stege
    Clinical and Experimental Photodermatology, Department of Dermatology, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, Germany
    J Photochem Photobiol B 65:105-8. 2001
    ..Photolyase encapsulated in liposomes may have in the near future a broad use as an active ingredient in modern skin care products...
  98. ncbi Light-induced activation of class II cyclobutane pyrimidine dimer photolyases
    Asako Okafuji
    Institut fur Physikalische Chemie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    DNA Repair (Amst) 9:495-505. 2010
    ....
  99. ncbi Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UVB radiation
    Jun Hidema
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980 8577, Japan
    Plant J 50:70-9. 2007
    ..However, further protection from UVB-induced damage may require the genetic enhancement of other systems as well...
  100. ncbi What is adenine doing in photolyase?
    Angela Acocella
    Dipartimento di Chimica G Ciamician, Universita di Bologna, V F Selmi 2, 40126, Bologna, Italy
    J Phys Chem B 114:4101-6. 2010
    ..In principle, knowledge of the details of the electron transfer dynamics and of the important role of polarization effects can be exploited to improve the efficiency of the repair mechanism in artificial systems...
  101. ncbi DNA (6-4) photolyases reduce Dewar isomers for isomerization into (6-4) lesions
    Andreas F Glas
    Center for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig Maximilians University Munich, Butenandtstr 5 13, 81377 Munich, Germany
    J Am Chem Soc 132:3254-5. 2010
    ..Repair of the Dewar valence isomers by (6-4) photolyases proceeds via an enzyme catalyzed ring-opening reaction of the Dewar lesion to the (6-4) photoproduct...

Research Grants10

  1. DNA REPAIR AND ITS RELATIONSHIP TO CARCINOGENESIS
    Errol Friedberg; Fiscal Year: 2001
    ..There is reason to believe that the proteins encoded by these genes operate in a different chromatin-modulating complex, and it is proposed to isolate and characterize this putative complex as well. ..
  2. Interrogation of systems level mechanisms controlling DNA repair processes
    Nitin S Baliga; Fiscal Year: 2010
    ..e. a predictive model for regulatory mechanisms for repair. This basic model will serve as a template for designing systems approaches to model higher complexities of eukaryotic repair processes. ..
  3. Signal Transduction in Enterococci
    Marta Perego; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  4. Role of E2F2 in eugenol-induced melanoma growth inhibit*
    Rita Ghosh; Fiscal Year: 2006
    ..This proposal addresses this very core of cancer chemoprevention that aims to reduce the national burden of high cost of treatment and loss of productive life. ..
  5. Protein Aspartate Phosphatases in Development
    Marta Perego; Fiscal Year: 2006
    ..The role of the Rap phosphatases not involved in sporulation will be investigated by microbiological and genetic approaches ..
  6. Kinetics of Oligomeric Protein Structure Formation
    YVONNE GINDT; Fiscal Year: 2003
    ..Finally, a detailed mechanism for formation of oligomeric structure in our model system will be developed. ..
  7. DNA REPAIR AND CANCER PRONE HEREDITARY HUMAN DISEASE
    Errol Friedberg; Fiscal Year: 2002
    ....
  8. MOUSE MODELS OF DNA REPAIR - DEFECTIVE HUMAN DISEASES
    Errol Friedberg; Fiscal Year: 2011
    ..abstract_text> ..
  9. DNA Damage Clusters: Repair in Mammalian Cells
    Betsy Sutherland; Fiscal Year: 2004
    ..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..
  10. FUNCTION OF THE S CEREVISIAE PHR1 GENE AND PHOTOLYASE
    GWENDOLYN SANCAR; Fiscal Year: 2004
    ..Ultimately this knowledge may help us to identify individuals at high risk for sunlight-induced cancers, as well as other forms of cancer. ..