cholesterol 7 alpha hydroxylase

Summary

Summary: A membrane-bound cytochrome P450 enzyme that catalyzes the 7-alpha-hydroxylation of CHOLESTEROL in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP7, converts cholesterol to 7-alpha-hydroxycholesterol which is the first and rate-limiting step in the synthesis of BILE ACIDS.

Top Publications

  1. ncbi Regulation of cholesterol-7alpha-hydroxylase: BAREly missing a SHP
    Roger A Davis
    Cell and Molecular Biology Laboratory, Department of Biology, San Diego State University, San Diego, CA 92182, USA
    J Lipid Res 43:533-43. 2002
  2. ncbi Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha
    D J Peet
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 75235 9050, USA
    Cell 93:693-704. 1998
  3. ncbi Thyroid hormone receptor beta-deficient mice show complete loss of the normal cholesterol 7alpha-hydroxylase (CYP7A) response to thyroid hormone but display enhanced resistance to dietary cholesterol
    H Gullberg
    Laboratory of Developmental Biology, Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
    Mol Endocrinol 14:1739-49. 2000
  4. ncbi A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis
    B Goodwin
    Department of Molecular Endocrinology, Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina 27709, USA
    Mol Cell 6:517-26. 2000
  5. ncbi Differential regulation of bile acid homeostasis by the farnesoid X receptor in liver and intestine
    Insook Kim
    Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Lipid Res 48:2664-72. 2007
  6. pmc A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene
    Kwang Hoon Song
    Department of Microbiology, Immunology and Biochemistry, Northeastern Ohio University College of Medicine, 4209 State Route 44, Rootstown, OH 44272, USA
    J Biol Chem 281:10081-8. 2006
  7. ncbi Variable association between genetic variation in the CYP7 gene promoter and plasma lipoproteins in three Canadian populations
    R A Hegele
    Department of Medicine, Blackburn Cardiovascular Genetics Laboratory, John P Robarts Research Institute, University of Western Ontario, 406 100 Perth Drive, Ont, N6A 5K8, London, Canada
    Atherosclerosis 154:579-87. 2001
  8. ncbi Requirement for thyroid hormone receptor beta in T3 regulation of cholesterol metabolism in mice
    Hjalmar Gullberg
    Department of Cell and Molecular Biology, Karolinska Institute, S 171 77 Stockholm, Sweden
    Mol Endocrinol 16:1767-77. 2002
  9. ncbi Fibroblast growth factor 15 functions as an enterohepatic signal to regulate bile acid homeostasis
    Takeshi Inagaki
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Metab 2:217-25. 2005
  10. ncbi PGC-1alpha activates CYP7A1 and bile acid biosynthesis
    Dong Ju Shin
    Department of Molecular Biology and Biochemistry, University of California, Irvine California 92697 3900, USA
    J Biol Chem 278:50047-52. 2003

Detail Information

Publications200 found, 100 shown here

  1. ncbi Regulation of cholesterol-7alpha-hydroxylase: BAREly missing a SHP
    Roger A Davis
    Cell and Molecular Biology Laboratory, Department of Biology, San Diego State University, San Diego, CA 92182, USA
    J Lipid Res 43:533-43. 2002
    ..These inflammatory cytokines, which include tumor necrosis factor alpha and interleukin-1beta, act upon liver parenchymal cells causing a rapid repression of the CYP7A1 gene...
  2. ncbi Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha
    D J Peet
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 75235 9050, USA
    Cell 93:693-704. 1998
    ..These results demonstrate the existence of a physiologically significant feed-forward regulatory pathway for sterol metabolism and establish the role of LXR alpha as the major sensor of dietary cholesterol...
  3. ncbi Thyroid hormone receptor beta-deficient mice show complete loss of the normal cholesterol 7alpha-hydroxylase (CYP7A) response to thyroid hormone but display enhanced resistance to dietary cholesterol
    H Gullberg
    Laboratory of Developmental Biology, Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
    Mol Endocrinol 14:1739-49. 2000
    ..The latter results lend strong support to the concept that TRs may exert regulatory effects in vivo independent of T3...
  4. ncbi A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis
    B Goodwin
    Department of Molecular Endocrinology, Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina 27709, USA
    Mol Cell 6:517-26. 2000
    ..This bile acid-activated regulatory cascade provides a molecular basis for the coordinate suppression of CYP7A1 and other genes involved in bile acid biosynthesis...
  5. ncbi Differential regulation of bile acid homeostasis by the farnesoid X receptor in liver and intestine
    Insook Kim
    Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Lipid Res 48:2664-72. 2007
    ..These data provide evidence that FXR-mediated repression of bile acid synthesis requires the complementary actions of FXR in both liver and intestine and reveal mechanistic differences in feedback repression of CYP7A1 and CYP8B1...
  6. pmc A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene
    Kwang Hoon Song
    Department of Microbiology, Immunology and Biochemistry, Northeastern Ohio University College of Medicine, 4209 State Route 44, Rootstown, OH 44272, USA
    J Biol Chem 281:10081-8. 2006
    ..These results suggest that Prox1 is a novel co-regulator of HNF4alpha that may play a key role in the regulation of bile acid synthesis and gluconeogenesis in the liver...
  7. ncbi Variable association between genetic variation in the CYP7 gene promoter and plasma lipoproteins in three Canadian populations
    R A Hegele
    Department of Medicine, Blackburn Cardiovascular Genetics Laboratory, John P Robarts Research Institute, University of Western Ontario, 406 100 Perth Drive, Ont, N6A 5K8, London, Canada
    Atherosclerosis 154:579-87. 2001
    ..The inconsistencies could be due to differences in genetic background or to unspecified environmental or genetic factors...
  8. ncbi Requirement for thyroid hormone receptor beta in T3 regulation of cholesterol metabolism in mice
    Hjalmar Gullberg
    Department of Cell and Molecular Biology, Karolinska Institute, S 171 77 Stockholm, Sweden
    Mol Endocrinol 16:1767-77. 2002
    ..That TRalpha1 only in some instances can substitute for TRbeta indicates that T3 regulation of physiological and molecular processes in the liver occurs in an isoform-specific fashion...
  9. ncbi Fibroblast growth factor 15 functions as an enterohepatic signal to regulate bile acid homeostasis
    Takeshi Inagaki
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Metab 2:217-25. 2005
    ..These studies define FGF15 and FGFR4 as components of a gut-liver signaling pathway that synergizes with SHP to regulate bile acid synthesis...
  10. ncbi PGC-1alpha activates CYP7A1 and bile acid biosynthesis
    Dong Ju Shin
    Department of Molecular Biology and Biochemistry, University of California, Irvine California 92697 3900, USA
    J Biol Chem 278:50047-52. 2003
    ..Our studies provide further evidence of the fundamental role played by PGC-1alpha in oxidative metabolism and define PGC-1alpha as a link between diabetes and bile acid metabolism...
  11. ncbi Genetic polymorphism in cytochrome P450 7A1 and risk of colorectal cancer: the Fukuoka Colorectal Cancer Study
    Tomoko Hagiwara
    Department of Preventive Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka, Japan
    Cancer Res 65:2979-82. 2005
    ....
  12. ncbi [Association of 7alpha-hydroxylase gene polymorphism with levels of plasma lipids]
    Bin Zhou
    West China Hospital Sichuan University, Department of Medical Genetics, Division of Morbid Genomics, Key Laboratory of Biotherapy of Human Disease, Ministry of Education, Chendu 610 041, P R China
    Yi Chuan 26:283-6. 2004
    ....
  13. ncbi Polymorphisms in CYP-7A1, not APOE, influence the change in plasma lipids in response to population dietary change in an 8 year follow-up; results from the Czech MONICA study
    Jaroslav A Hubacek
    Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, Prague, Czech Republic
    Clin Biochem 36:263-7. 2003
    ..To evaluate the influence of variation in the genes for apolipoprotein E (APOE; epsilon2, epsilon3, epsilon4) and cholesterol-7alpha hydroxylase (CYP-7A1; -204A-->C) on plasma lipid level changes...
  14. pmc Human cholesterol 7alpha-hydroxylase (CYP7A1) deficiency has a hypercholesterolemic phenotype
    Clive R Pullinger
    Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94143 0130, USA
    J Clin Invest 110:109-17. 2002
    ..Study of the kindred, which is of English and Celtic background, revealed that individuals heterozygous for the mutation are also hyperlipidemic, indicating that this is a codominant disorder...
  15. pmc Involvement of corepressor complex subunit GPS2 in transcriptional pathways governing human bile acid biosynthesis
    Sabyasachi Sanyal
    Department of Biosciences and Nutrition, Karolinska Institutet, Karolinska University Hospital, S 14157 Huddinge, Sweden
    Proc Natl Acad Sci U S A 104:15665-70. 2007
    ....
  16. ncbi Circulating intestinal fibroblast growth factor 19 has a pronounced diurnal variation and modulates hepatic bile acid synthesis in man
    T Lundåsen
    Metabolism Unit, Department of Endocrinology, Metabolism and Diabetes, Center for Nutrition and Toxicology, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    J Intern Med 260:530-6. 2006
    ..Through its systemic effects, circulating FGF19 may also mediate other known BA-dependent effects on lipid and carbohydrate metabolism...
  17. ncbi Activation of the nuclear receptor LXR by oxysterols defines a new hormone response pathway
    J M Lehmann
    Department of Molecular Endocrinology, Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 272:3137-40. 1997
    ..Our data provide evidence for a new hormonal signaling pathway that activates transcription in response to oxysterols and suggest that LXRs play a critical role in the regulation of cholesterol homeostasis...
  18. ncbi The negative effects of bile acids and tumor necrosis factor-alpha on the transcription of cholesterol 7alpha-hydroxylase gene (CYP7A1) converge to hepatic nuclear factor-4: a novel mechanism of feedback regulation of bile acid synthesis mediated by nucle
    E De Fabiani
    Dipartimento di Scienze Farmacologiche, , , Milano 20133, Italy
    J Biol Chem 276:30708-16. 2001
    ..Furthermore, the effects of CDCA and TNF-alpha converge to HNF-4, which binds to the BARE of CYP7A1, suggesting a link between the cascades elicited by bile acids and pro-inflammatory stimuli in the liver...
  19. ncbi Multiple mechanisms regulate circadian expression of the gene for cholesterol 7alpha-hydroxylase (Cyp7a), a key enzyme in hepatic bile acid biosynthesis
    Mitsuhide Noshiro
    Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan
    J Biol Rhythms 22:299-311. 2007
    ....
  20. ncbi Thyroid hormone-regulated target genes have distinct patterns of coactivator recruitment and histone acetylation
    Ying Liu
    Endocrinology Division, Department of Medicine, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue, Room B114, Baltimore, Maryland 21224, USA
    Mol Endocrinol 20:483-90. 2006
    ..Our findings suggest that thyroid hormone-regulated target genes may have distinct patterns of coactivator recruitment and histone acetylation that may enable highly specific regulation...
  21. pmc Loss of nuclear receptor SHP impairs but does not eliminate negative feedback regulation of bile acid synthesis
    Thomas A Kerr
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Dev Cell 2:713-20. 2002
    ..We conclude that input from three negative regulatory pathways controls bile acid synthesis. One is mediated by SHP, and two are SHP independent and invoked by liver damage and changes in bile acid pool size...
  22. ncbi A promoter polymorphism in cholesterol 7alpha-hydroxylase interacts with apolipoprotein E genotype in the LDL-lowering response to atorvastatin
    Kouji Kajinami
    Lipid Research Laboratory, Division of Endocrinology Metabolism and Molecular Biology, Tufts New England Medical Center, Boston, USA
    Atherosclerosis 180:407-15. 2005
    ..We concluded that the CYP7A1 A-204C promoter variant was associated with poor response to atorvastatin, which were additively enhanced by common variants in another locus, APOE...
  23. ncbi Regulation of the human cholesterol 7alpha-hydroxylase gene (CYP7A1) by thyroid hormone in transgenic mice
    Victor A B Drover
    Canadian Institutes of Health Research Group in Molecular and Cell Biology of Lipids, Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Endocrinology 145:574-81. 2004
    ..The data indicate that thyroid hormone can repress the human CYP7A1 gene in transgenic mice, but this effect is dependent on gender in this in vivo model...
  24. ncbi Cloning of the human cholesterol 7 alpha-hydroxylase gene (CYP7) and localization to chromosome 8q11-q12
    J C Cohen
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235
    Genomics 14:153-61. 1992
    ....
  25. ncbi The A-204C polymorphism in the cholesterol 7alpha-hydroxylase (CYP7A1) gene determines the cholesterolemia responsiveness to a high-fat diet
    J Kovar
    Institute for Clinical and Experimental Medicine, Laboratory for Atherosclerosis Research, Vídenská 1058 9, 140 21 Prague 4, Czech Republic
    Physiol Res 53:565-8. 2004
    ..Therefore, the polymorphism in the cholesterol 7alpha-hydroxylase promotor region seems to be involved in the determination of cholesterol and LDL-C responsiveness to a dietary fat challenge...
  26. ncbi Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene
    D T Molowa
    Department of Biochemical Regulation, Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
    Biochemistry 31:2539-44. 1992
    ..We suggest that the region between -432 and -220 functions as a cell-specific enhancer whose activity is controlled, in part, by HNF-3...
  27. ncbi The pharmacological exploitation of cholesterol 7alpha-hydroxylase, the key enzyme in bile acid synthesis: from binding resins to chromatin remodelling to reduce plasma cholesterol
    Federica Gilardi
    Dipartimento di Scienze Farmacologiche, Universita degli Studi di Milano, Milan, Italy
    Pharmacol Ther 116:449-72. 2007
    ..In recent years, new drugs have been designed based on recent discoveries of the regulatory network, thus confirming the position of CYP7A1 as a focus for innovative pharmacological intervention...
  28. ncbi Nuclear receptor regulation of genes involved in bile acid metabolism
    John T Moore
    GlaxoSmithKline, Research Triangle Park, NC 27709, USA
    Crit Rev Eukaryot Gene Expr 12:119-35. 2002
    ..These studies highlight the dynamic interplay between nuclear receptors in the regulation of specific metabolic pathways...
  29. ncbi Cross-talk between thyroid hormone receptor and liver X receptor regulatory pathways is revealed in a thyroid hormone resistance mouse model
    Koshi Hashimoto
    Department of Medicine and Molecular Science, Graduate School of Medicine, Gunma University, Maebashi, Gunma 371 8511, Japan
    J Biol Chem 281:295-302. 2006
    ..Electromobility shift analysis revealed that LXR/RXR heterodimers bound to the DR+4 element in the presence of MUT but not WT TR-beta. A mechanism for cross-talk, and potential antagonism, between TR-beta and LXR-alpha is proposed...
  30. pmc Role of an mSin3A-Swi/Snf chromatin remodeling complex in the feedback repression of bile acid biosynthesis by SHP
    Jongsook Kim Kemper
    Department of Molecular and Integrative Physiology, University of Illinois, Urbana, IL 61801, USA
    Mol Cell Biol 24:7707-19. 2004
    ..Our study establishes the first link between a Swi/Snf complex and regulation of cholesterol metabolism...
  31. ncbi Hepatocyte growth factor signaling pathway inhibits cholesterol 7alpha-hydroxylase and bile acid synthesis in human hepatocytes
    Kwang Hoon Song
    Department of Microbiology, Immunology and Biochemistry, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA
    Hepatology 46:1993-2002. 2007
    ....
  32. pmc Functional specificities of Brm and Brg-1 Swi/Snf ATPases in the feedback regulation of hepatic bile acid biosynthesis
    Ji Miao
    Department of Molecular and Integrative Physiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Mol Cell Biol 29:6170-81. 2009
    ....
  33. ncbi Linkage of the cholesterol 7alpha-hydroxylase gene and low-density lipoprotein cholesterol conditional on apolipoprotein E association: the National Heart, Lung, and Blood Institute Family Heart Study
    Jing Ping Lin
    Office of Biostatistics Research, National Heart, Lung and Blood Institute, National Institutes of Health, Salt Lake City, UT 84108 3528, USA
    Chin Med J (Engl) 118:362-9. 2005
    ..We report here an investigation of the relationship between LDL-C and cholesterol 7alpha-hydroxylase (CYP7), as well as apoE and low-density lipoprotein receptor (LDLR), three pivotal genes in LDL metabolism...
  34. ncbi Effects of dietary beta-cyclodextrin in hypercholesterolaemic rats
    Victoria García-Mediavilla
    Department of Physiology, University of Leon, 24071 Leon, Spain
    Pharmacol Toxicol 92:94-9. 2003
    ..Addition of beta-cyclodextrin to a cholesterol-rich diet results in a triglyceride-lowering action, enhancement of bile acid synthesis and excretion, and normalization of biliary lipid secretion, but produces a marked hepatotoxic effect...
  35. ncbi Regulation of bile acid synthesis by the nuclear receptor Rev-erbalpha
    Hélène Duez
    Institut Pasteur de Lille, Departement d Atherosclerose, Lille, France INSERM, U545, Lille, France
    Gastroenterology 135:689-98. 2008
    ..Here, we investigate a potential role for Rev-erbalpha in the control of bile acid metabolism via the regulation of the neutral bile acid synthesis pathway...
  36. ncbi CYP7A1 A-278C polymorphism affects the response of plasma lipids after dietary cholesterol or cafestol interventions in humans
    Maaike K Hofman
    TNO Prevention and Health, Gaubius Laboratory, Leiden, The Netherlands
    J Nutr 134:2200-4. 2004
    ..In conclusion, the CYP7A1 polymorphism has a small but significant effect on the increase in plasma HDL cholesterol and plasma total cholesterol after an increased intake of dietary cholesterol and cafestol, respectively...
  37. ncbi Molecular basis for feedback regulation of bile acid synthesis by nuclear receptors
    T T Lu
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 75390, USA
    Mol Cell 6:507-15. 2000
    ..These results reveal an elaborate autoregulatory cascade mediated by nuclear receptors for the maintenance of hepatic cholesterol catabolism...
  38. pmc CPF: an orphan nuclear receptor that regulates liver-specific expression of the human cholesterol 7alpha-hydroxylase gene
    M Nitta
    Biology Department, Tularik Inc, Two Corporate Drive, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 96:6660-5. 1999
    ..Cotransfection of a CPF expression plasmid and a CYP7A reporter gene resulted in specific induction of CYP7A-directed transcription. These observations suggest that CPF is a key regulator of human CYP7A gene expression in the liver...
  39. ncbi Common polymorphisms in the CYP7A1 gene do not contribute to variation in rates of bile acid synthesis and plasma LDL cholesterol concentration
    Anna Abrahamsson
    Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institute at Huddinge University Hospital, SE 14186 Stockholm, Sweden
    Atherosclerosis 182:37-45. 2005
    ..It is concluded that common polymorphisms of the CYP7A1 gene do not contribute to variation in cholesterol 7alpha-hydroxylase activity, rates of bile acid synthesis and plasma LDL cholesterol concentration in humans...
  40. ncbi A distinct thyroid hormone response element mediates repression of the human cholesterol 7alpha-hydroxylase (CYP7A1) gene promoter
    Victor A B Drover
    Canadian Institutes of Health Research Group in Molecular and Cell Biology of Lipids, and Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
    Mol Endocrinol 16:14-23. 2002
    ..The results indicate that T3-dependent repression of human CYP7A1 gene expression is mediated via a novel site in the human CYP7A1 gene promoter...
  41. ncbi Genetic variation in the rate-limiting enzyme in cholesterol catabolism (cholesterol 7alpha-hydroxylase) influences the progression of atherosclerosis and risk of new clinical events
    Maaike K Hofman
    TNO Prevention and Health, Gaubius Laboratory, Leiden, The Netherlands
    Clin Sci (Lond) 108:539-45. 2005
    ....
  42. ncbi Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase
    M Noshiro
    Department of Biochemistry, School of Dentistry, Hiroshima University, Japan
    FEBS Lett 268:137-40. 1990
    ..The deduced amino acid sequence showed 82% similarity to rat P-450ch7 alpha. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s...
  43. pmc Differential expression of hepatic genes involved in cholesterol homeostasis in high- and low-responding strains of laboratory opossums
    Jeannie Chan
    Southwest National Primate Research Center, Department of Genetics, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245 0549, USA
    Metabolism 57:718-24. 2008
    ..Low levels of CYP27A1, ABCG5, and ABCG8 expression in the liver may contribute to hypercholesterolemia in high-responding opossums...
  44. ncbi Disrupted coordinate regulation of farnesoid X receptor target genes in a patient with cerebrotendinous xanthomatosis
    Akira Honda
    Ibaraki Prefectural Institute of Public Health, Mito, Ibaraki 310 0852, Japan
    J Lipid Res 46:287-96. 2005
    ..The mechanism of the disruption may be explained by a normally stimulated FXR pathway attributable to markedly increased bile alcohols with activation of HNF4alpha caused by reduced bile acids in CTX liver...
  45. ncbi Effects of bile salt flux variations on the expression of hepatic bile salt transporters in vivo in mice
    Henk Wolters
    Center for Liver, Digestive and Metabolic Diseases, Groningen University Institute for Drug Exploration, Groningen, The Netherlands
    J Hepatol 37:556-63. 2002
    ..We investigated the physiological relevance of this regulation by evaluating transporter expression in mice experiencing different transhepatic bile salt fluxes...
  46. pmc Cholic acid mediates negative feedback regulation of bile acid synthesis in mice
    Jia Li-Hawkins
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9046, USA
    J Clin Invest 110:1191-200. 2002
    ..Cholate restores CYP7A1 regulation in vivo and in vitro. The results implicate cholate as an important negative regulator of bile acid synthesis and provide preliminary evidence for ligand-specific gene activation by a nuclear receptor...
  47. ncbi Coordinate transcriptional regulation of bile acid homeostasis and drug metabolism
    Jyrki J Eloranta
    Laboratory of Molecular Gastroenterology and Hepatology, Department of Internal Medicine, University Hospital, CH 8091 Zurich, Switzerland
    Arch Biochem Biophys 433:397-412. 2005
    ..The role of transcriptional cofactors, such as SRC-1 and PGC-1, in mediating the gene-specific effects of individual nuclear receptors is becoming increasingly evident...
  48. pmc Peroxisome proliferator-activated receptor-gamma coactivator-1alpha activation of CYP7A1 during food restriction and diabetes is still inhibited by small heterodimer partner
    Dong Ju Shin
    Department of Molecular Biology and Biochemistry, School of Biological Sciences, Center for Diabetes Research and Treatment, University of California, Irvine, CA 92697 3900, USA
    J Biol Chem 283:15089-96. 2008
    ....
  49. ncbi Dietary fish oil up-regulates cholesterol 7alpha-hydroxylase mRNA in mouse liver leading to an increase in bile acid and cholesterol excretion
    Annie M Berard
    Laboratoire de Biochimie et de Biologie Moleculaire, Universite Victor Segalen Bordeaux 2, Zone Nord Case 49 146, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France
    FEBS Lett 559:125-8. 2004
    ..On the other hand, we demonstrated that bile acid and cholesterol excretion were two-fold increased. These results show that LC omega-3 fatty acids control cholesterol metabolism in mice at a new endpoint...
  50. ncbi Barley beta-glucan lowers serum cholesterol based on the up-regulation of cholesterol 7alpha-hydroxylase activity and mRNA abundance in cholesterol-fed rats
    Jeong Lye Yang
    Research Institute of Human Ecology, Changwon University, Changwon 641 773, Korea
    J Nutr Sci Vitaminol (Tokyo) 49:381-7. 2003
    ..The results of this study suggest that the hypocholesterolemic effects of both beta-glucan and a waxy barley diet may be due to the enhancement of CYP7A1 expression resulting from increased fecal excretion of bile acids...
  51. ncbi Resistance of SHP-null mice to bile acid-induced liver damage
    Li Wang
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 278:44475-81. 2003
    ..These results identify an unexpected role for SHP in hepatotoxicity and suggest new approaches to modulating effects of chronically elevated bile acids in cholestasis...
  52. ncbi Identification of gene-selective modulators of the bile acid receptor FXR
    Isabelle Dussault
    Division of Molecular Medicine, The Beckman Research Institute, The City of Hope National Medical Center, The Gonda Diabetes and Genetic Research Center, Duarte, California 91010, USA
    J Biol Chem 278:7027-33. 2003
    ..Given the ability of BAR to regulate several lipid homeostatic pathways, the identification of gene-selective BAR modulators have important implications for the development of improved cholesterol lowering agents...
  53. ncbi Repression of farnesoid X receptor during the acute phase response
    Min Sun Kim
    Department of Medicine, University of California San Francisco, Metabolism Section, Medical Service, Department of Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 278:8988-95. 2003
    ....
  54. ncbi Ligand-activated pregnane X receptor interferes with HNF-4 signaling by targeting a common coactivator PGC-1alpha. Functional implications in hepatic cholesterol and glucose metabolism
    Sonali Bhalla
    Department of Molecular and Integrative Physiology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    J Biol Chem 279:45139-47. 2004
    ....
  55. ncbi Leptin induces the hepatic high density lipoprotein receptor scavenger receptor B type I (SR-BI) but not cholesterol 7alpha-hydroxylase (Cyp7a1) in leptin-deficient (ob/ob) mice
    Thomas Lundåsen
    Metabolism Unit, Center for Metabolism and Endocrinology, Department of Medicine and Molecular Nutrition Unit, Center for Nutrition and Toxicology, Novum, Karolinska Institutet at Huddinge University Hospital, S 141 86 Stockholm, Sweden
    J Biol Chem 278:43224-8. 2003
    ..Our findings indicate that leptin is an important regulator of hepatic SR-BI expression and, thus, HDL cholesterol levels, whereas it does not stimulate Cyp7a1 and bile acid synthesis...
  56. ncbi FXR-mediated down-regulation of CYP7A1 dominates LXRalpha in long-term cholesterol-fed NZW rabbits
    Guorong Xu
    Medical Service, Veteran s Administration Medical Center, East Orange, NJ 07018, USA
    J Lipid Res 44:1956-62. 2003
    ..Thus, in rabbits, when FXR and LXRalpha are activated simultaneously, the inhibitory effect of FXR overrides the stimulatory effect of LXRalpha to suppress CYP7A1 mRNA expression...
  57. ncbi Farnesoid X receptor agonists suppress hepatic apolipoprotein CIII expression
    Thierry Claudel
    UR545 INSERM, Departement d Atherosclerose, Institut Pasteur de Lille, Lille, France
    Gastroenterology 125:544-55. 2003
    ..Apolipoprotein CIII (Apo CIII) is a determinant of serum triglyceride metabolism. In this study, we investigated whether activators of the nuclear farnesoid X receptor (FXR) modulate Apo CIII gene expression...
  58. ncbi Taurine prevents hypercholesterolemia in ovariectomized rats fed corn oil but not in those fed coconut oil
    Taro Kishida
    Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama 790 8566, Japan
    J Nutr 133:2616-21. 2003
    ..These results indicate that the effect of taurine on ovarian hormone deficiency-induced changes in cholesterol metabolism is influenced by the type of dietary fatty acids...
  59. ncbi Enzyme-resistant fractions of beans lowered serum cholesterol and increased sterol excretions and hepatic mRNA levels in rats
    Kyu Ho Han
    Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Lipids 38:919-24. 2003
    ..05) higher than in the CP group. These results suggest that AS and TS have a serum cholesterol-lowering function due to the enhanced levels of hepatic SR-B1 and cholesterol 7alpha-hydroxylase mRNA...
  60. ncbi Coordinated control of cholesterol catabolism to bile acids and of gluconeogenesis via a novel mechanism of transcription regulation linked to the fasted-to-fed cycle
    Emma De Fabiani
    Dipartimento di Scienze Farmacologiche, Facolta di Farmacia, Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy
    J Biol Chem 278:39124-32. 2003
    ....
  61. ncbi Inhibition of ileal bile acid transport lowers plasma cholesterol levels by inactivating hepatic farnesoid X receptor and stimulating cholesterol 7 alpha-hydroxylase
    Hai Li
    Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ, USA
    Metabolism 53:927-32. 2004
    ..Thus, a new balance was reached in the liver, where increased bile acid synthesis compensated for diminished ileal bile acid absorption to maintain the circulating enterohepatic bile acid pool...
  62. ncbi Change in hepatic and plasma bile acid contents and its regulatory gene expression in the chicken embryo
    Momoka Sato
    Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
    Comp Biochem Physiol B Biochem Mol Biol 150:344-7. 2008
    ..The present results showed that the mRNA expression of CYP7A1 and synthesis of bile acids was low in embryonic stages, suggesting that FXR might be a key regulator of CYP7A1 gene expression in the chicken embryo...
  63. ncbi Dietary rhubarb (Rheum rhaponticum) stalk fibre does not lower plasma cholesterol levels in diabetic rats
    Sukhinder Kaur Cheema
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Br J Nutr 89:201-6. 2003
    ..These results suggest that although a rhubarb-fibre-enriched diet increased cyp7a activity in BBdp rats, there was no apparent therapeutic benefit in terms of lowering plasma cholesterol concentrations...
  64. pmc Increased cholesterol 7alpha-hydroxylase expression and size of the bile acid pool in the lactating rat
    Clavia Ruth Wooton-Kee
    Graduate Ctr for Toxicology, University of Kentucky College of Medicine, Lexington, KY 40536, USA
    Am J Physiol Gastrointest Liver Physiol 294:G1009-16. 2008
    ..The increase in Cyp7a1 expression PP provides a mechanism for the increase in the bile acid pool...
  65. pmc Liver receptor homolog 1 transcriptionally regulates human bile salt export pump expression
    Xiulong Song
    Department of Biomedical and Pharmaceutical Sciences, Center for Pharmacogenomics and Molecular Therapy, College of Pharmacy, University of Rhode Island, Kingston, RI, USA
    J Lipid Res 49:973-84. 2008
    ..These results suggest that LRH-1 plays a supporting role to FXR in maintaining hepatic bile acid levels by coordinately regulating CYP7A1 and BSEP for bile acid synthesis and elimination, respectively...
  66. ncbi Removal of the bile acid pool upregulates cholesterol 7alpha-hydroxylase by deactivating FXR in rabbits
    Guorong Xu
    Medical Service, G1 Lab 15A, Veterans Affairs Medical Center, 385 Tremont Avenue, East Orange, NJ 07018 1095, USA
    J Lipid Res 43:45-50. 2002
    ..Further, lack of the high affinity ligand supply was associated with downregulation of hepatic FXR mRNA levels...
  67. ncbi Hypercholesterolemia and changes in lipid and bile acid metabolism in male and female cyp7A1-deficient mice
    Sandra K Erickson
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    J Lipid Res 44:1001-9. 2003
    ..Taken together, the data suggest that cyp7A1 deficiency results in a proatherogenic phenotype in both genders and leads to a prolithogenic phenotype in females...
  68. ncbi Increased production of apolipoprotein B-containing lipoproteins in the absence of hyperlipidemia in transgenic mice expressing cholesterol 7alpha-hydroxylase
    J H Miyake
    Mammalian Cell and Molecular Biology Laboratory, San Diego State University, San Diego, California 92182 4614, USA
    J Biol Chem 276:23304-11. 2001
    ..These findings emphasize the important homeostatic role that CYP7A1 plays in balancing the anabolic lipoprotein assembly/secretion pathway with the cholesterol catabolic bile acid synthetic pathway...
  69. ncbi Feeding unsaponifiable compounds from rice bran oil does not alter hepatic mRNA abundance for cholesterol metabolism-related proteins in hypercholesterolemic rats
    K Nagao
    Division of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School Kyushu University, Fukuoka, Japan
    Biosci Biotechnol Biochem 65:371-7. 2001
    ..These results show that the UC in RBO leads to a decreased serum cholesterol concentration by interrupting the absorption of intestinal hydrophobic compounds rather than by modifying cholesterol metabolism in the liver...
  70. ncbi In vivo and in vitro studies on the regulatory link between 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol 7 alpha-hydroxylase in rat liver
    M Boll
    , GSF National Research Center for Environment and Health, Neuherberg, Germany
    Z Naturforsch C 54:371-82. 1999
    ..The in vitro studies supply evidence that the effects of cholesterol and bile acid excess or deficiency are direct and do not involve accessory changes of hormone levels or mediators...
  71. ncbi Studies on LXR- and FXR-mediated effects on cholesterol homeostasis in normal and cholic acid-depleted mice
    J Wang
    Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden
    J Lipid Res 47:421-30. 2006
    ..Interaction between FXR- and LXR-mediated stimuli might also regulate expression of liver ABCG5/G8...
  72. ncbi Elevated cholesterol metabolism and bile acid synthesis in mice lacking membrane tyrosine kinase receptor FGFR4
    C Yu
    Department of Biochemistry and Biophysics, Texas A and M University, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, Texas 77030 3303, USA
    J Biol Chem 275:15482-9. 2000
    ....
  73. ncbi Activation of the farnesoid X receptor improves lipid metabolism in combined hyperlipidemic hamsters
    Stefan Bilz
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 8012, USA
    Am J Physiol Endocrinol Metab 290:E716-22. 2006
    ..Our studies identify activators of FXR as promising new tools in the therapy of hypertriglyceridemic states, including the insulin resistance syndrome and type 2 diabetes...
  74. pmc Targeted deletion of Gpbar1 protects mice from cholesterol gallstone formation
    Galya Vassileva
    Department of Discovery Technologies, Schering Plough Research Institute, Kenilworth, NJ 07033, USA
    Biochem J 398:423-30. 2006
    ..These results suggest that Gpbar1 plays a critical role in the formation of gallstones, possibly via a regulatory mechanism involving the cholesterol 7alpha-hydroxylase pathway...
  75. ncbi Disruption of the sterol 27-hydroxylase gene in mice results in hepatomegaly and hypertriglyceridemia. Reversal by cholic acid feeding
    J J Repa
    Departments of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 275:39685-92. 2000
    ..These studies confirm the importance of CYP27 in bile acid synthesis and they reveal an unexpected function of the enzyme in triacylglycerol metabolism...
  76. ncbi Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers
    J J Repa
    Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Science 289:1524-9. 2000
    ..Thus, these RXR heterodimers serve as key regulators of cholesterol homeostasis by governing reverse cholesterol transport from peripheral tissues, bile acid synthesis in liver, and cholesterol absorption in intestine...
  77. ncbi Digestion-resistant fraction from soybean [Glycine max (L.) Merrill] induces hepatic LDL receptor and CYP7A1 expression in apolipoprotein E-deficient mice
    Sung Hee Han
    Department of Agricultural Chemistry, College of Life and Environmental Sciences, Korea University, Seoul 136 701, South Korea
    J Nutr Biochem 17:682-8. 2006
    ..The cholesterol was reduced by multiple mechanisms such as increased hepatic cholesterol uptake, cholesterol degradation into bile acids and bile acid excretion...
  78. ncbi Hamsters predisposed to sucrose-induced cholesterol gallstones (LPN strain) are more resistant to excess dietary cholesterol than hamsters that are not sensitive to cholelithiasis induction
    M Souidi
    Laboratoire de Physiologie de la Nutrition-INRA, , F-91405 Orsay Cedex, France
    J Nutr 131:1803-11. 2001
    ..These results highlight the usefulness of this two-strain model, which offers some analogy with the inverse association between the predisposition to cholelithiasis and the risk of atherosclerosis in humans...
  79. ncbi Fibrates suppress bile acid synthesis via peroxisome proliferator-activated receptor-alpha-mediated downregulation of cholesterol 7alpha-hydroxylase and sterol 27-hydroxylase expression
    S M Post
    Gaubius Laboratory, TNO-PG, Leiden, The Netherlands
    Arterioscler Thromb Vasc Biol 21:1840-5. 2001
    ..A decreased bile acid production by PPARalpha-mediated downregulation of cholesterol 7alpha-hydroxylase and sterol 27-hydroxylase may contribute to the increased risk of gallstone formation after fibrate treatment...
  80. pmc Tissue- and nuclear receptor-specific function of the C-terminal LXXLL motif of coactivator NCoA6/AIB3 in mice
    Qingtian Li
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell Biol 27:8073-86. 2007
    ....
  81. ncbi SC-435, an ileal apical sodium-codependent bile acid transporter inhibitor alters mRNA levels and enzyme activities of selected genes involved in hepatic cholesterol and lipoprotein metabolism in guinea pigs
    Kristy Lynn West
    Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA
    J Nutr Biochem 16:722-8. 2005
    ..These results suggest that both SC-435 monotherapy and combination therapy lower LDL cholesterol concentrations by altering both hepatic cholesterol homeostasis and the intravascular processing of lipoproteins in guinea pigs...
  82. ncbi Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression
    M Zhang
    Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio 44272, USA
    J Biol Chem 276:41690-9. 2001
    ..Bile acids repress human CYP8B1 transcription by reducing the transactivation activity of HNF4alpha through interaction of HNF4alpha with SHP and reduction of HNF4alpha expression in the liver...
  83. ncbi Identification of a nuclear receptor for bile acids
    M Makishima
    Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9050, USA
    Science 284:1362-5. 1999
    ..These results demonstrate a mechanism by which bile acids transcriptionally regulate their biosynthesis and enterohepatic transport...
  84. ncbi Cholecystectomy prevents expansion of the bile acid pool and inhibition of cholesterol 7alpha-hydroxylase in rabbits fed cholesterol
    G Xu
    Medical Service, Veterans Affairs Medical Center, East Orange, NJ 07018-1095, USA
    J Lipid Res 42:1438-43. 2001
    ..Removal of the gallbladder prevented expansion of the bile acid pool after cholesterol feeding as seen in intact rabbits because ileal bile acid transport did not increase. As a result, cholesterol 7alpha-hydroxylase was not inhibited...
  85. ncbi Nuclear receptor-mediated repression of human cholesterol 7alpha-hydroxylase gene transcription by bile acids
    W Chen
    Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA
    J Lipid Res 42:1402-12. 2001
    ..Differential regulation of FTF and SHP expression by bile acids may explain the wide variation in CYP7A1 expression and the rate of bile acid synthesis and regulation in different species...
  86. ncbi Differences in the regulation of the classical and the alternative pathway for bile acid synthesis in human liver. No coordinate regulation of CYP7A1 and CYP27A1
    Ingemar Bjorkhem
    Department of Pharmaceutical Biosciences, Division of Biochemistry, University of Uppsala, Box 578, S 751 23 Uppsala, Sweden
    J Biol Chem 277:26804-7. 2002
    ..The results underline that marked species differences may exist in mechanisms for control of synthesis of bile acids and cholesterol homeostasis...
  87. ncbi Studies on the cholesterol-free mouse: strong activation of LXR-regulated hepatic genes when replacing cholesterol with desmosterol
    Maura Heverin
    Division of Clinical Chemistry, Karolinska University Hospital, Huddinge, S 14186, Sweden
    Arterioscler Thromb Vasc Biol 27:2191-7. 2007
    ..Characterization of cholesterol homeostasis in male mice with a genetic inactivation of 3beta-hydroxysteroid-delta24-reductase, causing replacement of almost all cholesterol with desmosterol...
  88. ncbi Effect of high plant sterol-enriched diet and cholesterol absorption inhibitor, SCH 58235, on plant sterol absorption and plasma concentrations in hypercholesterolemic wild-type Kyoto rats
    Ashok K Batta
    Department of Medicine, UMDNJ NJ Medical School, Newark, NJ 07103, USA
    Metabolism 54:38-48. 2005
    ....
  89. ncbi Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
    Metabolism 52:636-42. 2003
    ..We conclude that, in the rat, like CYP7A1, CYP7B1 demonstrates diurnal rhythm and is regulated by bile acids and cholesterol...
  90. ncbi Protein-expression profiles in mouse blood-plasma following acute whole-body exposure to (137)Cs gamma rays
    Kanokporn Noy Rithidech
    Pathology Department, Stony Brook University, Stony Brook, New York 11794 8691, USA
    Int J Radiat Biol 85:432-47. 2009
    ..To compare the pattern of protein-expression profiles in blood-plasma after exposure of CBA/CaJ mice to 0 or 3 Gy of (137)Cs gamma rays...
  91. ncbi [Effect of hypolipidemic treatment on the composition of bile and the risk or cholesterol gallstone disease]
    A Zak
    IV interni klinika 1 LF UK a VFN, Praha
    Cas Lek Cesk 146:24-34. 2007
    ....
  92. ncbi Up-regulation of acyl-coenzyme A:cholesterol acyltransferase (ACAT) in nephrotic syndrome
    Nosratola D Vaziri
    Division of Nephrology and Hypertension, Department of Medicine, University of California, Irvine, Irvine, California, USA
    Kidney Int 61:1769-75. 2002
    ..Therefore, we hypothesized that NS may result in up-regulation of hepatic ACAT...
  93. ncbi Polymorphisms at cholesterol 7alpha-hydroxylase, apolipoproteins B and E and low density lipoprotein receptor genes in patients with gallbladder stone disease
    Zhao Yan Jiang
    Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
    World J Gastroenterol 10:1508-12. 2004
    ....
  94. ncbi Bile acid reabsorption inhibitors (BARI): novel hypolipidemic drugs
    Werner Kramer
    TD Metabolism, Sanofi Aventis Deutschland GmbH, Industriepark Hochst, Building G 879, 65926 Frankfurt am Main, Germany
    Curr Med Chem 13:997-1016. 2006
    ..The review article covers the mechanistic and therapeutic principles of the approach and presents an overview on the molecular target, the discovery of specific inhibitors and respective optimization strategies...
  95. ncbi The role of alpha1-fetoprotein transcription factor/LRH-1 in bile acid biosynthesis: a known nuclear receptor activator that can act as a suppressor of bile acid biosynthesis
    Antonio Del Castillo-Olivares
    Department of Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
    J Biol Chem 279:16813-21. 2004
    ....
  96. ncbi Polymorphism in the coding part of the sterol 12alpha-hydroxylase gene does not explain the marked differences in the ratio of cholic acid and chenodeoxycholic acid in human bile
    A Abrahamsson
    Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institute, Stockholm
    Scand J Clin Lab Invest 65:595-600. 2005
    ..The aim of this study was to investigate whether this marked difference could be due to a genetic polymorphism in the gene of the CYP8B1...
  97. ncbi Coordinate regulation of xenobiotic and bile acid homeostasis by pregnane X receptor
    J Staudinger
    Department of Pharmacology and Toxicology, University of Kansas Medical Center, Breidenthal Building, Kansas City, KS 66160 7417, USA
    Drug Metab Dispos 29:1467-72. 2001
    ..Taken together, these data demonstrate a pivotal role for PXR in the regulation of drug-induced hepatomegaly and in the metabolism (CYP3A), transport (Oatp2), biosynthesis (Cyp7a1), and excretion of xenobiotics and bile acids in vivo...
  98. ncbi Regulation of diurnal variation of cholesterol 7alpha-hydroxylase (CYP7A1) activity in healthy subjects
    J Kovar
    Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    Physiol Res 59:233-8. 2010
    ..Moreover, short-term treatment with cholestyramine resulted in about 10 % suppression of glycemia throughout the day. Our results suggest that insulin is involved in the regulation of diurnal variation of CYP7A1 activity in humans...
  99. pmc A novel bile acid-activated vitamin D receptor signaling in human hepatocytes
    Shuxin Han
    Department of Integrative Medical Sciences, Northeastern Ohio University s Colleges of Medicine and Pharmacy, 4209 State Route 44, Rootstown, Ohio 44272, USA
    Mol Endocrinol 24:1151-64. 2010
    ..This membrane VDR-signaling pathway may be activated by bile acids to inhibit bile acid synthesis as a rapid response to protect hepatocytes from cholestatic liver injury...
  100. ncbi Osthol regulates hepatic PPAR alpha-mediated lipogenic gene expression in alcoholic fatty liver murine
    Fan Sun
    Department of Pharmacology, Medical College of Soochow University, Suzhou 215123, Jiangsu Province, China
    Phytomedicine 17:669-73. 2010
    ..It was concluded that osthol might regulate the gene expressions of DGAT, HMG-CoA reductase and CYP7A via increasing the PPAR alpha mRNA expression...
  101. ncbi Reduction in bile acid pool causes delayed liver regeneration accompanied by down-regulated expression of FXR and c-Jun mRNA in rats
    Xiushan Dong
    The First Hospital of Shanxi Medical University, Taiyuan, 030001, China
    J Huazhong Univ Sci Technolog Med Sci 30:55-60. 2010
    ..The reduction in the bile acid pool size was found to delay the liver regeneration, which may be caused by the down-regulation of FXR and c-Jun expression...

Research Grants33

  1. New pathways regulating bile acid homeostasis and cholestatic liver diseases
    Li Wang; Fiscal Year: 2013
    ..Fully defining the regulation of bile acid metabolism, including bile acid synthesis and catabolism, is essential for further advances in diagnosis, management, and prevention of cholestatic liver diseases. ..
  2. Regulation of Bile Acid Synthesis by Nuclear Receptors in Vivo
    Gregorio Gil; Fiscal Year: 2012
    ..The successful completion of this study will provide us with new insights into the molecular mechanisms involved in the regulation of bile acid biosynthesis. ..
  3. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2009
    ..Drugs targeting to nuclear receptor and signaling pathways, and miRNA antagomirs may be developed for treating metabolic liver diseases. ..
  4. Effects of sleep deprivation and high fat diet on human CYP7A1 circadian rhythm
    JESSICA MARIE FERRELL; Fiscal Year: 2013
    ..The proposed studies aim to understand the contribution of deregulated circadian liver metabolism in the progression of diabetes and obesity. ..
  5. REGULATION OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 1999
    ....
  6. PREMATURE WEANING AND ADULT CHOLESTEROL HOMEOSTASIS
    DONALD MC NAMARA; Fiscal Year: 1993
    ....
  7. CELLULAR REGULATION OF HEPATOCYTE EXCRETORY FUNCTION
    EDWARD OLINGER; Fiscal Year: 1980
    ..Microsomal P448 (benzo-a-pyrene hydroxylase). Microsomal O-aminophenol-UDPGA-transferase. Triglyceride release, cholesterol biosynthesis, HMG-CoA-reductase. Cyclic-AMP and Cyclic GMP concentrations...
  8. Mechanism of activation of the JNK pathway by bile acids
    Seema Gupta; Fiscal Year: 2006
    ....
  9. CATABOLISM AND EXCRETION OF CHOLESTEROL AND BILE ACIDS
    David Loose; Fiscal Year: 2002
    ..In this respect the applicant anticipates that the results will impact upon the treatment of cardiovascular diseases well as having significance for fundamental biological questions of the control of cholesterol homeostasis. ..
  10. BILE ACID METABOLISM AND PLASMA CHOLESTEROL REGULATION
    DAVID SPADY; Fiscal Year: 2000
    ....
  11. CHOLESTEROL REGULATION BY CEREALS AND FUNGAL PRODUCTS
    WARREN BURGER; Fiscal Year: 1980
    ..The pronounced effects observed by us which are detailed in this proposal have implications for human nutrition and possible control of cardiovascular diseases in which cholesterol plays a key role...
  12. INBORN ERRORS IN BILE ACID SYNTHESIS
    Sarah Shefer; Fiscal Year: 2000
    ..e. 7DHCR, lathosterol 5-dehydrogenase, cholesterol 7 alpha hydroxylase, sterol 27-hydroxylase, and 5 beta cholestane, 3 alpha, 7 beta, 12 alpha, 25-tetrol 24S-hydroxylase (..
  13. REGULATION OF CHOLESTEROL BIOSYNTHESIS AND DEGRADATION
    GENE NESS; Fiscal Year: 1993
    ..The degree of similarity of mechanisms of hormonal and dietary regulation of these enzymes will be determined...
  14. Regulation of Bile Acid by an alpha 1-AT Peptide
    Gregorio Gil; Fiscal Year: 2007
    ..4. Characterization of the alpha1-AT promoter and its self-regulation by the C-36 peptide. The successful completion of this project may provide an important link between cholesterol homeostasis and inflammation. ..
  15. Regulation of Bile Acid Synthesis by Nuclear Receptors
    John Y L Chiang; Fiscal Year: 2010
    ..This study could contribute to understanding the mechanisms of metabolic diseases caused by dysregulation of bile acid synthesis and develop therapeutic agents to treat dyslipidemia, liver diseases, obesity and type II diabetes. ..
  16. Agilent 6890 GC/5973 mass detector for Profiling
    Roger Davis; Fiscal Year: 2005
    ..The projected use of each investigator is shown in table 1 and will account for 100% time use. Therefore, we have no alternative equipment for this essential technique. ..
  17. APO B TRANSLOCATION AND DEGRADATION
    Roger Davis; Fiscal Year: 2007
    ..We are confident that our proposed studies will provide new insights that will add further proof to the feasibility and advance the therapeutic potential of controlling the apo B processing pathway. ..
  18. FTF and gpCYP7A1 are targets of protein kinases
    Diane Stroup; Fiscal Year: 2006
    ..The data will serve as a basis for further studies using the proteomics techniques of 2D HPLC and SELDI. ..
  19. MOLECULAR REGULATION OF FXR ACTIVITY
    Jongsook Kim Kemper; Fiscal Year: 2010
    ..The studies may also facilitate the design of therapeutic agents for treating these metabolic disorders. ..
  20. Prevent HIV protease inhibitor-induced atherosclerosis by Berberine
    HUIPING ROSE ZHOU; Fiscal Year: 2010
    ..Completion of these specific aims will help identify and establish new therapeutic strategies for HIV infection. ..
  21. Regulation of cholesterol catabolism by bile acids
    JONGSOOK KEMPER; Fiscal Year: 2008
    ..abstract_text> ..
  22. FUNCTIONS OF THE HEPATIC GAMMA-GLUTAMYL CYCLE
    Nazzareno Ballatori; Fiscal Year: 2007
    ....
  23. HIV Protease Inhibitors, the Unfolded Protein Response and Atherosclerosis
    Huiping Zhou; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  24. PATHOGENESIS OF ANTICONVULSANT HYPERSENSITIVITY SYNDROME
    JAMES LEEDER; Fiscal Year: 2003
    ..It is anticipated that the basic experimental paradigm employed for these studies can also be applied to other idiosyncratic toxicities with suspected drug bioactivation and immune etiologies. ..
  25. CNS defects in a murine Zellweger syndrome model
    Phyllis Faust; Fiscal Year: 2007
    ....
  26. Immune Reactants, Cholesterol and Atherosclerosis
    ALLISON REISS; Fiscal Year: 2006
    ..abstract_text> ..
  27. CNS DEFECT IN A MURINE ZELLWEGER SYNDROME MODEL
    Phyllis Faust; Fiscal Year: 2002
    ....
  28. Host-Microbial Control of Deoxycholate Producton
    Paul Dawson; Fiscal Year: 2005
    ..Aim 3: To cross the Slcl0a2 null allele into additional mouse strains as a first step toward cloning the genes responsible controlling deoxycholate production (colonization by the bai operon bacteria). ..
  29. METHYLMERCURY TRANSPORT ACROSS CELL MEMBRANES
    Nazzareno Ballatori; Fiscal Year: 2005
    ..Evaluate the molecular mechanism by which NAC stimulates renal excretion of MeHg. IV. Test the hypothesis that urinary excretion of MeHg following an NAC oral challenge may be used as a new biomarker of MeHg exposure. ..
  30. Ontogeny of Drug Bioactivation and Idiosyncratic ADRs
    JAMES LEEDER; Fiscal Year: 2007
    ..abstract_text> ..