25 hydroxyvitamin d3 1 alpha hydroxylase

Summary

Summary: A mitochondrial cytochrome P450 enzyme that catalyzes the 1-alpha-hydroxylation of 25-hydroxyvitamin D3 (also known as 25-hydroxycholecalciferol) in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP27B1 gene, converts 25-hydroxyvitamin D3 to 1-alpha,25-dihydroxyvitamin D3 which is the active form of VITAMIN D in regulating bone growth and calcium metabolism. This enzyme is also active on plant 25-hydroxyvitamin D2 (ergocalciferol).

Top Publications

  1. ncbi Down-regulation by nuclear factor kappaB of human 25-hydroxyvitamin D3 1alpha-hydroxylase promoter
    Regina Ebert
    Orthopedic Department, University of Wuerzburg, Wuerzburg, Germany
    Mol Endocrinol 18:2440-50. 2004
  2. ncbi Extrarenal expression of 25-hydroxyvitamin d(3)-1 alpha-hydroxylase
    D Zehnder
    Division of Medical Sciences, University of Birmingham, Birmingham, United Kingdom B15 2TH
    J Clin Endocrinol Metab 86:888-94. 2001
  3. ncbi 25-hydroxy-vitamin d metabolism in human colon cancer cells during tumor progression
    P Bareis
    Department of Pathophysiology, University of Vienna Medical School, Vienna, A-1090, Austria
    Biochem Biophys Res Commun 285:1012-7. 2001
  4. ncbi Response of the 5'-flanking region of the human 25-hydroxyvitamin D 1alpha-hydroxylase gene to physiological stimuli using a transgenic mouse model
    I Hendrix
    Hanson Institute, Frome Rd, Adelaide 5000, SA, Australia
    J Mol Endocrinol 34:237-45. 2005
  5. ncbi Expression and activity of 25-hydroxyvitamin D-1 alpha-hydroxylase are restricted in cultures of human syncytiotrophoblast cells from preeclamptic pregnancies
    Lorenza Díaz
    Department of Reproductive Biology, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico Distrito Federal, 14000, Mexico
    J Clin Endocrinol Metab 87:3876-82. 2002
  6. ncbi Regulation of Vitamin D hydroxylases gene expression by 1,25-dihydroxyvitamin D3 and cyclic AMP in cultured human syncytiotrophoblasts
    Euclides Avila
    Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion, Salvador Zubiran, Vasco de Quiroga No 15, Tlalpan 14000, Mexico D F, Mexico
    J Steroid Biochem Mol Biol 103:90-6. 2007
  7. ncbi A promoter polymorphism of the CYP27B1 gene is associated with Addison's disease, Hashimoto's thyroiditis, Graves' disease and type 1 diabetes mellitus in Germans
    Elizabeth Ramos Lopez
    Department of Internal Medicine I, Division of Endocrinology, University Hospital Frankfurt, Frankfurt am Main, Germany
    Eur J Endocrinol 151:193-7. 2004
  8. ncbi 25-hydroxyvitamin D(3)-1alpha-hydroxylase expression in normal and pathological parathyroid glands
    Ulrika Segersten
    Department of Surgical Sciences, Endocrine Unit, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
    J Clin Endocrinol Metab 87:2967-72. 2002
  9. ncbi Biological actions of extra-renal 25-hydroxyvitamin D-1alpha-hydroxylase and implications for chemoprevention and treatment
    Kelly Townsend
    Division of Medical Sciences, Institute of Biomedical Research, The University of Birmingham, Birmingham B15 2TH, UK
    J Steroid Biochem Mol Biol 97:103-9. 2005
  10. ncbi Reduced 1alpha-hydroxylase activity in human prostate cancer cells correlates with decreased susceptibility to 25-hydroxyvitamin D3-induced growth inhibition
    J Y Hsu
    Department of Medicine, Stanford University School of Medicine, California 94305, USA
    Cancer Res 61:2852-6. 2001

Research Grants

Detail Information

Publications161 found, 100 shown here

  1. ncbi Down-regulation by nuclear factor kappaB of human 25-hydroxyvitamin D3 1alpha-hydroxylase promoter
    Regina Ebert
    Orthopedic Department, University of Wuerzburg, Wuerzburg, Germany
    Mol Endocrinol 18:2440-50. 2004
    ..Down-regulation of 1alpha-hydroxylase promoter through NFkappaB signaling may contribute to the pathogenesis of inflammation-associated osteopenia/osteoporosis...
  2. ncbi Extrarenal expression of 25-hydroxyvitamin d(3)-1 alpha-hydroxylase
    D Zehnder
    Division of Medical Sciences, University of Birmingham, Birmingham, United Kingdom B15 2TH
    J Clin Endocrinol Metab 86:888-94. 2001
    ..However, the discrete patterns of staining in these tissues emphasizes a possible role for 1 alpha-hydroxylase as an intracrine modulator of vitamin D function in peripheral tissues...
  3. ncbi 25-hydroxy-vitamin d metabolism in human colon cancer cells during tumor progression
    P Bareis
    Department of Pathophysiology, University of Vienna Medical School, Vienna, A-1090, Austria
    Biochem Biophys Res Commun 285:1012-7. 2001
    ....
  4. ncbi Response of the 5'-flanking region of the human 25-hydroxyvitamin D 1alpha-hydroxylase gene to physiological stimuli using a transgenic mouse model
    I Hendrix
    Hanson Institute, Frome Rd, Adelaide 5000, SA, Australia
    J Mol Endocrinol 34:237-45. 2005
    ..It also responds in the kidney to the physiological regulators of development and ageing...
  5. ncbi Expression and activity of 25-hydroxyvitamin D-1 alpha-hydroxylase are restricted in cultures of human syncytiotrophoblast cells from preeclamptic pregnancies
    Lorenza Díaz
    Department of Reproductive Biology, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico Distrito Federal, 14000, Mexico
    J Clin Endocrinol Metab 87:3876-82. 2002
    ..These results support the role of placenta as a contributor to the abnormalities observed in calcium metabolism in PE...
  6. ncbi Regulation of Vitamin D hydroxylases gene expression by 1,25-dihydroxyvitamin D3 and cyclic AMP in cultured human syncytiotrophoblasts
    Euclides Avila
    Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion, Salvador Zubiran, Vasco de Quiroga No 15, Tlalpan 14000, Mexico D F, Mexico
    J Steroid Biochem Mol Biol 103:90-6. 2007
    ..The overall data suggest that calcitriol downregulates CYP27B1 expression via a cAMP-dependent signaling pathway, whereas upregulates 24-hydroxylase gene expression through a VDR-dependent mechanism...
  7. ncbi A promoter polymorphism of the CYP27B1 gene is associated with Addison's disease, Hashimoto's thyroiditis, Graves' disease and type 1 diabetes mellitus in Germans
    Elizabeth Ramos Lopez
    Department of Internal Medicine I, Division of Endocrinology, University Hospital Frankfurt, Frankfurt am Main, Germany
    Eur J Endocrinol 151:193-7. 2004
    ..We therefore investigated two single nucleotide polymorphisms in the CYP27B1 hydroxylase gene for an association with Addison's disease, Hashimoto's thyroiditis, Graves' disease and type 1 diabetes mellitus...
  8. ncbi 25-hydroxyvitamin D(3)-1alpha-hydroxylase expression in normal and pathological parathyroid glands
    Ulrika Segersten
    Department of Surgical Sciences, Endocrine Unit, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
    J Clin Endocrinol Metab 87:2967-72. 2002
    ..The findings imply that in addition to feedback control by circulating 1,25(OH)(2)D(3) levels, parathyroid cells may also be influenced by local 1alpha -hydroxylase activity with possible growth regulatory and differentiating effects...
  9. ncbi Biological actions of extra-renal 25-hydroxyvitamin D-1alpha-hydroxylase and implications for chemoprevention and treatment
    Kelly Townsend
    Division of Medical Sciences, Institute of Biomedical Research, The University of Birmingham, Birmingham B15 2TH, UK
    J Steroid Biochem Mol Biol 97:103-9. 2005
    ..The role of 1alpha-hydroxylase in protection against cancer is likely to be more complicated and may involve anti-tumor immune responses...
  10. ncbi Reduced 1alpha-hydroxylase activity in human prostate cancer cells correlates with decreased susceptibility to 25-hydroxyvitamin D3-induced growth inhibition
    J Y Hsu
    Department of Medicine, Stanford University School of Medicine, California 94305, USA
    Cancer Res 61:2852-6. 2001
    ..This finding has ramifications for both the prevention and therapy of prostate cancer with vitamin D compounds...
  11. pmc Parathyroid hormone activation of the 25-hydroxyvitamin D3-1alpha-hydroxylase gene promoter
    H L Brenza
    Department of Biochemistry, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:1387-91. 1998
    ..These results indicate that parathyroid hormone stimulates 25-hydroxyvitamin D3-1alpha-hydroxylase by acting on the promoter of the 1alpha-hydroxylase gene...
  12. ncbi Effects of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 on cytokine production by human decidual cells
    Katie N Evans
    Division of Medical Sciences, Institute of Biomedical Research, The University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Biol Reprod 75:816-22. 2006
    ....
  13. ncbi Differential regulation of vitamin D receptor and its ligand in human monocyte-derived dendritic cells
    Martin Hewison
    Department of Medical Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    J Immunol 170:5382-90. 2003
    ..In conclusion, differential regulation of endogenous 1 alpha 25(OH)(2)D(3) ligand and its nuclear receptor appear to be important regulators of DC biology and represent potential targets for the manipulation of DC function...
  14. pmc Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts
    David Barrera
    Departamento de Biologia de la Reproduccion, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No, 15, Tlalpan 14000, Mexico, DF, Mexico
    Reprod Biol Endocrinol 6:3. 2008
    ..In the present work we studied calcitriol actions upon human chorionic gonadotropin (hCG) secretion and expression in cultured trophoblasts, as well as vitamin D receptor (VDR) and CYP27B1 immunolocalization in placental villi...
  15. ncbi Comprehensive association analysis of the vitamin D pathway genes, VDR, CYP27B1, and CYP24A1, in prostate cancer
    Crystal N Holick
    Cancer Prevention Program, Fred Hutchinson Cancer Research Center, USA
    Cancer Epidemiol Biomarkers Prev 16:1990-9. 2007
    ..Our findings suggest that polymorphisms in the VDR gene may be associated with prostate cancer risk and, therefore, that the vitamin D pathway might have an etiologic role in the development of prostate cancer...
  16. ncbi Cycling and early pregnant endometrium as a site of regulated expression of the vitamin D system
    P Vigano
    Department of Obstetrics, Gynecology and Neonatology, Fondazione Policlinico Mangiagalli Regina Elena Hospital, University of Milano, Milano, Italy
    J Mol Endocrinol 36:415-24. 2006
    ..Abnormalities of this system are present in endometriosis...
  17. ncbi 1alpha,25-dihydroxyvitamin D3 downregulates CYP27B1 and induces CYP24A1 in colon cells
    Daniel Lechner
    Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Mol Cell Endocrinol 263:55-64. 2007
    ..In turn, accumulation of 1alpha,25-(OH)2D3 will induce 24-hydroxylation. We conclude that, although extrarenal and renal metabolic pathways for Vitamin D are similar, malignancy of tumor cells determines extent of Vitamin D catabolism...
  18. ncbi Role of 24-hydroxylase in vitamin D3 growth response of OVCAR-3 ovarian cancer cells
    Susanna Miettinen
    Department of Cell Biology, Medical School, University of Tampere, Tampere, Finland
    Int J Cancer 108:367-73. 2004
    ..The possible mitogenic effects of vitamin D should be considered when evaluating treatment of ovarian cancer with vitamin D...
  19. ncbi 25-Hydroxyvitamin D-1alpha-hydroxylase activity is diminished in human prostate cancer cells and is enhanced by gene transfer
    Lyman W Whitlatch
    Department of Medicine, Vitamin D, Skin and Bone Research Laboratory, Endocrine Section, Boston University School of Medicine, Boston, MA 02118, USA
    J Steroid Biochem Mol Biol 81:135-40. 2002
    ....
  20. ncbi Human mammary epithelial cells express CYP27B1 and are growth inhibited by 25-hydroxyvitamin D-3, the major circulating form of vitamin D-3
    Carly M Kemmis
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    J Nutr 136:887-92. 2006
    ....
  21. pmc Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching
    Akiko Murayama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo, Japan
    EMBO J 23:1598-608. 2004
    ..Thus, the present study deciphers a novel mechanism of ligand-induced transrepression by nuclear receptor via co-regulator switching...
  22. ncbi Targeted disruption of the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in ras-transformed keratinocytes demonstrates that locally produced 1alpha,25-dihydroxyvitamin D3 suppresses growth and induces differentiation in an autocrine fashion
    Dao Chao Huang
    Department of Medicine, Royal Victoria Hospital and McGill University, Montreal, Quebec, Canada
    Mol Cancer Res 1:56-67. 2002
    ....
  23. ncbi Cloning of human 25-hydroxyvitamin D-1 alpha-hydroxylase and mutations causing vitamin D-dependent rickets type 1
    G K Fu
    Department of Pediatrics, Child Health Research Center, San Francisco, California 94121, USA
    Mol Endocrinol 11:1961-70. 1997
    ..These findings establish the molecular genetic basis of VDDR-1, establish a novel means for its study in keratinocytes, and provide the sequence of the key enzyme in the biological activation of vitamin D...
  24. ncbi Constitutive expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in a transformed human proximal tubule cell line: evidence for direct regulation of vitamin D metabolism by calcium
    R Bland
    Department of Medicine, Institute of Clinical Research, University of Birmingham, United Kingdom
    Endocrinology 140:2027-34. 1999
    ..These data suggest that direct effects of calcium on proximal tubule cells may be an important feature of the regulation of renal 1,25-(OH)2D3 production...
  25. ncbi Metabolism of vitamin D3 in the placental tissue of normal and preeclampsia complicated pregnancies and premature births
    D Fischer
    Department of of Gynaecology and Obstetrics, University of Schleswig Holstein, Campus Lubeck, Germany
    Clin Exp Obstet Gynecol 34:80-4. 2007
    ....
  26. ncbi Increased 25-hydroxyvitamin D3 1alpha-hydroxylase and reduced 25-hydroxyvitamin D3 24-hydroxylase expression in parathyroid tumors--new prospects for treatment of hyperparathyroidism with vitamin d
    Pamela Correa
    Department of Surgical Sciences, Endocrine Unit, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
    J Clin Endocrinol Metab 87:5826-9. 2002
    ..These findings should be considered in future development of vitamin D analogues for treatment of HPT...
  27. pmc Vitamin D induces innate antibacterial responses in human trophoblasts via an intracrine pathway
    N Liu
    Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Biol Reprod 80:398-406. 2009
    ..2% protection, respectively). These observations indicate that 1,25(OH)(2)D can function as an intracrine regulator of CAMP in trophoblasts, and may thus provide a novel mechanism for activation of innate immune responses in the placenta...
  28. pmc The ontogeny of 25-hydroxyvitamin D(3) 1alpha-hydroxylase expression in human placenta and decidua
    Daniel Zehnder
    Division of Medical Sciences, The University of Birmingham, Queen Elizabeth Hospital, Birmingham, UK
    Am J Pathol 161:105-14. 2002
    ..These spatiotemporal data emphasize the potential importance of 1alpha-OHase during early fetoplacental life and, in particular, suggest an autocrine/paracrine immunomodulatory function for the enzyme...
  29. ncbi Inhibitors of vitamin D hydroxylases: structure-activity relationships
    Inge Schuster
    Institute of Pharmaceutical Chemistry, University Vienna, Althanstrasse 15, A 1090 Wien, Osterreich
    J Cell Biochem 88:372-80. 2003
    ..In the absence of crystal structures, our inhibitors are valuable tools to model and understand the active sites of vitamin D hydroxylases, resulting in the design of powerful, selective therapeutics...
  30. ncbi A haplotype of the CYP27B1 promoter is associated with autoimmune Addison's disease but not with Graves' disease in a UK population
    C E Jennings
    Institute of Human Genetics, School of Clinical Medical Sciences, University of Newcastle, Newcastle upon Tyne, UK
    J Mol Endocrinol 34:859-63. 2005
    ..We are able to confirm that a CYP27B1 promoter allele is associated with autoimmune Addison's disease, and extend this finding to include an associated promoter haplotype...
  31. ncbi Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with colorectal cancer
    B W Ogunkolade
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    Lancet 359:1831-2. 2002
    ....
  32. ncbi Metabolism of vitamin D3 in human osteoblasts: evidence for autocrine and paracrine activities of 1 alpha,25-dihydroxyvitamin D3
    Gerald J Atkins
    Department of Orthopaedics and Trauma, University of Adelaide, Adelaide, South Australia 5000, Australia
    Bone 40:1517-28. 2007
    ..Our results are therefore consistent with the reported benefits of maintaining a healthy vitamin D status in the elderly to reduce the risk of fractures...
  33. ncbi Altered pharmacokinetics of 1alpha,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3 in the blood and tissues of the 25-hydroxyvitamin D-24-hydroxylase (Cyp24a1) null mouse
    Sonoko Masuda
    Department of Biochemistry, Queen s University, Kingston Ontario, Canada K7L 3N6
    Endocrinology 146:825-34. 2005
    ..These results suggest that the absence of CYP24A1 or VDR retards catabolism of 1alpha,25(OH)2D3 and 25(OH)D3, reinforcing the physiological importance of CYP24A1 in vitamin D homeostasis...
  34. ncbi Effects of vitamin D compounds on renal and intestinal Ca2+ transport proteins in 25-hydroxyvitamin D3-1alpha-hydroxylase knockout mice
    Joost G J Hoenderop
    Department of Physiology, Nijmegen Centre for Molecular Life Sciences, University Medical Centre Nijmegen, The Netherlands
    Kidney Int 66:1082-9. 2004
    ..Newer vitamin D compounds retain the suppressive action of 1,25(OH)(2)D(3) on the parathyroid glands and may have less Ca(2+)-mobilizing activity, offering potentially safer therapies...
  35. pmc Altered endocrine and autocrine metabolism of vitamin D in a mouse model of gastrointestinal inflammation
    Nancy Liu
    Department of Orthopaedic Surgery, David Geffen School of Medicine, UCLA, 615 Charles E Young Drive South, Los Angeles, California 90095, USA
    Endocrinology 149:4799-808. 2008
    ..These data indicate that both local and endocrine synthesis of 1,25(OH)2D3 affect colitis in DSS-treated mice. Lack of Cyp27b1 exacerbates disease in this model, suggesting that similar effects may occur with vitamin D deficiency...
  36. ncbi Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D status
    William B Ogunkolade
    Center for Diabetes and Metabolic Medicine, Royal London Hospital, London E1 1BB, United Kingdom
    J Clin Endocrinol Metab 91:2612-7. 2006
    ..We previously reported that circulating 1,25(OH)(2)D contributed to determination of VDR-protein levels and VDR genotype was a determinant of both VDR mRNA and VDR-protein in South Asians...
  37. ncbi Dermal fibroblasts pretreated with a sterol Delta7-reductase inhibitor produce 25-hydroxyvitamin D3 upon UVB irradiation
    Katleen Vantieghem
    Laboratorium voor Experimentele Geneeskunde en Endocrinologie LEGENDO, Gasthuisberg, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium
    J Photochem Photobiol B 85:72-8. 2006
    ..The physiological relevance of dermal vitamin D3 photoproduction and its subsequent conversion into 25OHD3 remains elusive...
  38. ncbi Determinants of circulating 1,25-dihydroxyvitamin D3 levels: the role of renal synthesis and catabolism of vitamin D
    Paul H Anderson
    Endocrine Bone Laboratory, Hanson Institute, Adelaide, SA 5000, Australia
    J Steroid Biochem Mol Biol 89:111-3. 2004
    ..89, P < 0.001). These findings suggest that kidney CYP24 activity acts in concert with kidney CYP27B1 to control serum 1,25D levels and that serum 1,25D stimulates renal CYP24 expression by acting through the renal VDR...
  39. ncbi Vitamin D and placental-decidual function
    Katie N Evans
    Division of Medical Sciences, Institute of Biomedical Research, The University of Birmingham, Queen Elizabeth Hospital, Birmingham B15 2TH, United Kingdom
    J Soc Gynecol Investig 11:263-71. 2004
    ..In particular, we describe how induction of the vitamin D-activating enzyme 1alpha-hydroxylase in early gestation might provide a mechanism by which environmental or dietary vitamin D can influence fetal-placental development...
  40. ncbi Analysis of the vitamin D system in basal cell carcinomas (BCCs)
    Tanja Mitschele
    Universitäts Hautklinik, Universitätshautkliniken des Saarlandes, Homburg, Germany
    Lab Invest 84:693-702. 2004
    ..The function of alternative transcripts of 1alpha-OHase that we describe here for the first time in BCCs and NS and their effect on activity level has to be investigated in future experiments...
  41. ncbi Analysis of the vitamin D system in cutaneous squamous cell carcinomas
    Jörg Reichrath
    Department of Dermatology, The Saarland University Hospital, Homburg, Germany
    J Cutan Pathol 31:224-31. 2004
    ....
  42. ncbi Leptin corrects increased gene expression of renal 25-hydroxyvitamin D3-1 alpha-hydroxylase and -24-hydroxylase in leptin-deficient, ob/ob mice
    Ayako Matsunuma
    Department of Biochemistry, Ohu University School of Dentistry, Koriyama 963 8611, Japan
    Endocrinology 145:1367-75. 2004
    ..Therefore, low BMD in leptin-deficient mice appears to be related to stimulation of bone resorption by 1,25-(OH)(2)D(3)...
  43. ncbi The role of Vitamin D3 metabolism in prostate cancer
    Y R Lou
    Department of Anatomy, Medical School, SF 33014 University of Tampere, Finland
    J Steroid Biochem Mol Biol 92:317-25. 2004
    ..2004)]) and phytoestrogens may modulate the expression of Vitamin D metabolizing enzymes. In summary, the local metabolism of hormonal Vitamin D seems to play an important role in the development and progression of prostate cancer...
  44. pmc 20-Hydroxyvitamin D3, a product of vitamin D3 hydroxylation by cytochrome P450scc, stimulates keratinocyte differentiation
    Blazej Zbytek
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Invest Dermatol 128:2271-80. 2008
    ..These data imply that the previously unreported pathway of vitamin D3 metabolism by P450scc may have wider biological implications depending, for example, on the extent of adrenal gland or cutaneous metabolism...
  45. ncbi Epigenetic regulation of vitamin D hydroxylase expression and activity in normal and malignant human prostate cells
    Maya Khorchide
    Department of Pathophysiology, Medical University of Vienna, A 1090 Vienna, Waehringerguertel 18 20, Austria
    J Steroid Biochem Mol Biol 93:167-72. 2005
    ..This demonstrates for the first time that synthesis of 1,25-dihydroxycholecalciferol in human prostate tumors could be reinitiated by epigenetic regulators...
  46. ncbi Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response
    Philip T Liu
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 311:1770-3. 2006
    ..These data support a link between TLRs and vitamin D-mediated innate immunity and suggest that differences in ability of human populations to produce vitamin D may contribute to susceptibility to microbial infection...
  47. ncbi In vitro comparison of the vitamin D endocrine system in 1,25(OH)2D3-responsive and -resistant melanoma cells
    Jörg Reichrath
    Department of Dermatology, The Saarland University Hospital, Homburg Germany
    Cancer Biol Ther 6:48-55. 2007
    ....
  48. ncbi The Vitamin D endocrine system of the gut--its possible role in colorectal cancer prevention
    Heide S Cross
    Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, A 1090 Vienna, Waehringer Guertel 18 20, Austria
    J Steroid Biochem Mol Biol 97:121-8. 2005
    ..These results and our observation that 17beta-estradiol can elevate CYP27B1 expression in rectal tissue of postmenopausal women, may underlie the observed protective effect of estrogens against colorectal cancer in females...
  49. ncbi Vitamin D(3) metabolism in human glioblastoma multiforme: functionality of CYP27B1 splice variants, metabolism of calcidiol, and effect of calcitriol
    Britta Diesel
    Institut fur Humangenetik, Theoretische Medizin, Universitat des Saarlandes, Saarbrucken, Germany
    Clin Cancer Res 11:5370-80. 2005
    ..A better understanding of the vitamin D(3) metabolism is required to evaluate its potential therapeutic value for cancers. Here, we set out to contribute to the understanding of vitamin D(3) metabolism in glioblastoma multiforme...
  50. ncbi 25-Hydroxyvitamin D(3) suppresses PTH synthesis and secretion by bovine parathyroid cells
    C S Ritter
    Renal Division, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Kidney Int 70:654-9. 2006
    ..Clotrimazole enhanced 24-hydroxylase mRNA induction, presumably by inhibiting catabolism of 25(OH)D(3). In conclusion, 25(OH)D(3) suppresses PTH synthesis by parathyroid cells, possibly by direct activation of the VDR...
  51. ncbi Ovariectomy worsens secondary hyperparathyroidism in mature rats during low-Ca diet
    Yan Zhang
    State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, Hong Kong, China
    Am J Physiol Endocrinol Metab 292:E723-31. 2007
    ..These results suggest that estrogen is necessary for the full adaptive response to LCD mediated by both PTH and 1,25(OH)2D3...
  52. ncbi Vitamin D status and breast cancer risk
    Kay W Colston
    Department of Clinical Biochemistry, St George s Hospital Medical School, London SW15, UK
    Anticancer Res 26:2573-80. 2006
    ....
  53. ncbi Fibroblast growth factor 23 impairs phosphorus and vitamin D metabolism in vivo and suppresses 25-hydroxyvitamin D-1alpha-hydroxylase expression in vitro
    Farzana Perwad
    Pediatrics, University of California San Francisco, San Francisco, California, USA
    Am J Physiol Renal Physiol 293:F1577-83. 2007
    ..These novel findings provide evidence that FGF-23 directly regulates renal 1alpha-hydroxylase gene expression via activation of the ERK1/2 signaling pathway...
  54. pmc Role of the vitamin D receptor in FGF23 action on phosphate metabolism
    Yoshio Inoue
    Department of Molecular Nutrition, Institute of Health Bioscience, The University of Tokushima Graduate School, Kuramoto Cho 3, Tokushima 770 8503, Japan
    Biochem J 390:325-31. 2005
    ..In contrast, the induction of 25-hydroxyvitamin D 24-hydroxylase and the reduction of serum 1,25(OH)2D3 levels induced by FGF23 are dependent on the VDR...
  55. ncbi 25(OH)D3 and 1,25(OH)2D3 serum concentration and breast tissue expression of 1alpha-hydroxylase, 24-hydroxylase and Vitamin D receptor in women with and without breast cancer
    Eduardo Carneiro de Lyra
    Instituto Brasileiro de Controle do Cancer, Av Alcântara Machado 2576, São Paulo SP CEP 03102 002, Brazil
    J Steroid Biochem Mol Biol 100:184-92. 2006
    ..A low 1,25(OH)2D3 serum concentration seems to be associated to breast cancer, however, the mechanism involved in this regulation is still unclear...
  56. ncbi Colon-specific regulation of vitamin D hydroxylases--a possible approach for tumor prevention
    Enikö Kállay
    Department of Pathophysiology, Medical University of Vienna, A 1090 Vienna, Wahringer Guertel 18 20, Austria
    Carcinogenesis 26:1581-9. 2005
    ....
  57. pmc Secreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent
    Theresa Berndt
    Department of Medicine, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    J Clin Invest 112:785-94. 2003
    ..Thus, sFRP-4 displays phosphatonin-like properties, because it is a circulating protein that promotes phosphaturia and hypophosphatemia and blunts compensatory increases in 1alpha, 25-dihydroxyvitamin D...
  58. ncbi Gene expression of vitamin D hydroxylase and megalin in the remnant kidney of nephrectomized rats
    Fumi Takemoto
    Kidney Center, Toranomon Hospital, Tokyo, Japan
    Kidney Int 64:414-20. 2003
    ....
  59. ncbi Renal expression of the sodium/phosphate cotransporter gene, Npt2, is not required for regulation of renal 1 alpha-hydroxylase by phosphate
    H S Tenenhouse
    Department of Pediatrics, McGill University Montreal Children s Hospital Research Institute, Montreal, Quebec, Canada H3H 1P3
    Endocrinology 142:1124-9. 2001
    ..Thus, intact renal Na/Pi cotransport is not required for the regulation of renal 1alpha-hydroxylase by Pi...
  60. ncbi Renal calcification in mice homozygous for the disrupted type IIa Na/Pi cotransporter gene Npt2
    Hien Chau
    Department of Biology, McGill University, Montreal, Quebec, Canada
    J Bone Miner Res 18:644-57. 2003
    ..In summary, we show that Npt2 gene ablation is associated with renal calcification and suggest that mutations in the NPT2 gene may contribute to nephrocalcinosis in a subset of patients with familial hypercalciuria...
  61. ncbi Phytoestrogens regulate vitamin D metabolism in the mouse colon: relevance for colon tumor prevention and therapy
    Enikö Kállay
    Department of Pathophysiology, University of Vienna Medical School, Vienna, Austria
    J Nutr 132:3490S-3493S. 2002
    ..Our data therefore suggest that an inverse correlation of soy product consumption with colon tumor incidence may be consequent to enhanced colonic synthesis of the antimitotic hormone 1,25-dihydroxyvitamin D3...
  62. ncbi 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis
    K Takeyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113, Japan
    Science 277:1827-30. 1997
    ..These results indicate that the negative feedback regulation of active vitamin D synthesis is mediated by 1alpha(OH)ase through liganded VDR...
  63. ncbi Inhibition of prostate cancer growth by vitamin D: Regulation of target gene expression
    Aruna V Krishnan
    Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    J Cell Biochem 88:363-71. 2003
    ..Further investigation of the role of calcitriol and its analogs for the therapy or chemoprevention of PCa is currently being pursued...
  64. ncbi Hormonal regulation of 25-hydroxyvitamin D3-1alpha-hydroxylase and 24-hydroxylase gene transcription in opossum kidney cells
    H J Armbrecht
    Geriatric Research, Education, and Clinical Center 11G JB, St Louis VA Medical Center, St Louis, MO 63125, USA
    Arch Biochem Biophys 409:298-304. 2003
    ..This suggests that there may be important posttranscriptional regulation of CYP24 mRNA in the kidney...
  65. ncbi Transcriptional inhibition of CYP24 by genistein
    Hesso Farhan
    Department of Pathophysiology, University of Vienna Medical School, Vienna, Austria
    Ann N Y Acad Sci 973:459-62. 2002
    ..In conclusion, our data argue for the use of genistein to resensitize prostate cancer cells to 1,25-D3; the addition of TSA may preserve the ability of these cells to synthesize 1,25-D3...
  66. ncbi Genistein inhibits vitamin D hydroxylases CYP24 and CYP27B1 expression in prostate cells
    Hesso Farhan
    Department of Pathophysiology, University of Vienna Medical School, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Steroid Biochem Mol Biol 84:423-9. 2003
    ..In summary, our results suggest that a combined effect of genistein and trichostatin A could increase the responsiveness of human prostate cancer cells to the antiproliferative action of 1,25-dihydroxyvitamin D(3)...
  67. pmc Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium
    Kevin D Healy
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:9733-7. 2003
    ..This in vivo study demonstrates that 1,25(OH)2D3 and calcium are both required for renal VDR mRNA expression above a basal level, furthering our understanding of the complex regulation of renal VDR by 1,25(OH)2D3 and calcium...
  68. ncbi Regulation of extrarenal vitamin D metabolism as a tool for colon and prostate cancer prevention
    Heide S Cross
    Institute of Pathophysiology, University of Vienna Medical School, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Recent Results Cancer Res 164:413-25. 2003
    ..We were able to demonstrate that genistein down-regulates expression of CYP24 to almost nil, which would result in enhancement of local 1,25-D3 levels and improved mitotic control of tumor cells...
  69. ncbi Clonal differences in expression of 25-hydroxyvitamin D(3)-1alpha-hydroxylase, of 25-hydroxyvitamin D(3)-24-hydroxylase, and of the vitamin D receptor in human colon carcinoma cells: effects of epidermal growth factor and 1alpha,25-dihydroxyvitamin D(3)
    Petra Bareis
    Department of Pathophysiology, University of Vienna Medical School, Austria
    Exp Cell Res 276:320-7. 2002
    ....
  70. ncbi Rescue of the pseudo-vitamin D deficiency rickets phenotype of CYP27B1-deficient mice by treatment with 1,25-dihydroxyvitamin D3: biochemical, histomorphometric, and biomechanical analyses
    Olivier Dardenne
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada
    J Bone Miner Res 18:637-43. 2003
    ..These results show that chronic treatment with the active 1,25(OH)2D3 metabolite is effective to rescue the PDDR phenotype of 1alpha-OHase mutant mice...
  71. ncbi Vitamin D analogs as therapeutic agents: a clinical study update
    J Ruth Wu-Wong
    Abbott Laboratories, R4CM Building AP52, 200 Abbott Park Rd, Abbott Park, IL 60064, USA
    Curr Opin Investig Drugs 5:320-6. 2004
    ..This review discusses recent progress on the use of vitamin D and its analogs in the management of HPT secondary to chronic renal disease...
  72. pmc Klotho prevents renal calcium loss
    R Todd Alexander
    Department of Physiology, Nijmegen Centre for Molecular Life Sciences, 6500 HB Nijmegen, The Netherlands
    J Am Soc Nephrol 20:2371-9. 2009
    ..This highlights the importance of klotho to the prevention of renal Ca(2+) loss, secondary hypervitaminosis D, osteopenia, and nephrocalcinosis...
  73. ncbi Expression of 25-hydroxyvitamin D3-24-hydroxylase mRNA in cultured human keratinocytes
    M L Chen
    Department of Medicine, Boston University Medical Center, Massachusetts
    Proc Soc Exp Biol Med 207:57-61. 1994
    ..These results demonstrate that 1 alpha,25(OH)2D3 up-regulates the expression of 24-OHase mRNA, and this may be an important first step in the initiation of catabolism of 1 alpha,25(OH)2D3 in human keratinocytes...
  74. ncbi cDNA cloning and characterization of a vitamin D3 hydroxylase-associated protein
    R A Ettinger
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 269:176-82. 1994
    ..VDHAP is a novel chick kidney-specific inner membrane protein of mitochondria, which associates with a fraction of the 1,25-dihydroxyvitamin D3-24R-hydroxylase and 25-hydroxyvitamin D3-1 alpha-hydroxylase...
  75. ncbi New developments in calcium and vitamin D metabolism
    C B Langman
    Department of Pediatrics, Northwestern University, Evanston, Illinois, USA
    Curr Opin Pediatr 12:135-9. 2000
    ....
  76. ncbi Autocrine metabolism of vitamin D in normal and malignant breast tissue
    Kelly Townsend
    Division of Medical Sciences, Institute of Biomedical Research, Endocrinology and Metabolism, University of Birmingham, Birmingham, United Kingdom
    Clin Cancer Res 11:3579-86. 2005
    ..The aim of this study was to investigate the significance of this in breast cancer...
  77. ncbi Calcitriol regulates the expression of the genes encoding the three key vitamin D3 hydroxylases and the drug-metabolizing enzyme CYP3A4 in the human fetal intestine
    Catherine Theodoropoulos
    Departement de Pharmacologie, Faculte de Medecine, Centre de Recherche, Centre Hospitalier de l Universite de Montreal, Universite de Montreal, Quebec, Canada
    Clin Endocrinol (Oxf) 58:489-99. 2003
    ..Total RNA was extracted and cDNA synthesized by RT-PCR. The cDNA obtained was amplified by radioactive PCR, the signal intensity evaluated by densitometric analyses and expressed in relation to the levels of GAPDH...
  78. ncbi Intestinal and renal adaptation to a low-Pi diet of type II NaPi cotransporters in vitamin D receptor- and 1alphaOHase-deficient mice
    Paola Capuano
    Institute of Physiology, University of Zurich, Zurich, Switzerland
    Am J Physiol Cell Physiol 288:C429-34. 2005
    ..On the basis of the results, we conclude that the regulation of NaPi cotransport in small intestine (via NaPi-IIb) and kidney (via NaPi-IIa) by low dietary intake of Pi cannot be explained by the 1,25(OH)2D-VDR axis...
  79. ncbi Epigenetic regulation of vitamin D converting enzymes
    Matthias Wjst
    Helmholtz Zentrum Munchen, Institute of Lung Biology and Disease, German Research Center for Environmental Health, Ingolstadter Landstr 1, Munchen, D 85764 Neuherberg, Germany
    J Steroid Biochem Mol Biol 121:80-3. 2010
    ..As PBLs represent an heterogeneous pool of cells, a further analysis of the seasonal methylation pattern in B or T cell subsets (or other tissues like liver or kidney) is warranted including an extended coverage of the CYP27B1 promotor...
  80. ncbi Low vitamin D levels in outpatient postmenopausal women from a rheumatology clinic in Madrid, Spain: their relationship with bone mineral density
    P Aguado
    Rheumatology and Clinical Biochemistry Divisions, H U La Paz, Madrid, Spain
    Osteoporos Int 11:739-44. 2000
    ..Vitamin D deficiency is a frequent finding in the postmenopausal women who attend a rheumatology outpatient clinic in Madrid. Vitamin D supplementation should therefore be considered in this population during the winter season...
  81. pmc 25-Hydroxyvitamin D3 1alpha-hydroxylase expression in breast cancer and use of non-1alpha-hydroxylated vitamin D analogue
    Ulrika Segersten
    Department of Surgical Sciences, Endocrine Unit, Uppsala University Hospital, SE 751 85 Uppsala, Sweden
    Breast Cancer Res 7:R980-6. 2005
    ..In addition, expression of the 25-hydroxyvitamin D3 24-hydroxylase (24-hydroxylase) and the vitamin D receptor (VDR) was investigated...
  82. ncbi Monocyte-derived cells express CYP27A1 and convert vitamin D3 into its active metabolite
    Eva Gottfried
    Department of Hematology and Oncology, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
    Biochem Biophys Res Commun 349:209-13. 2006
    ..Our results show that macrophages and dendritic cells are capable to perform both hydroxylation steps of the vitamin D3 metabolism suggesting a possible role of local 1,25(OH)2D3 synthesis by myeloid cells in the skin and gut...
  83. pmc Association of the vitamin D metabolism gene CYP27B1 with type 1 diabetes
    Rebecca Bailey
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Diabetes 56:2616-21. 2007
    ..Our aim was to study the association between the CYP27B1 and CYP24A1 gene polymorphisms and type 1 diabetes...
  84. ncbi 25-Hydroxyvitamin D3 1alpha-hydroxylase splice variants in breast cell lines MCF-7 and MCF-10
    D Fischer
    Klinik fur Frauenheilkunde und Geburtshilfe, Universitatsklinikum Schleswig Holstein, Campus Lubeck, Lubeck, Germany
    Cancer Genomics Proteomics 4:295-300. 2007
    ..With this study it is the first time that expression and alternative splicing of 1alpha-OHase in the human breast cancer cell line MCF-7 and thebenign breast cell line MCF-10A are described...
  85. ncbi 25-Hydroxyvitamin D requirement for maintaining skeletal health utilising a Sprague-Dawley rat model
    P H Anderson
    Endocrine Bone Research Laboratory, Hanson Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    J Steroid Biochem Mol Biol 103:592-5. 2007
    ..Such information is essential for developing nutritional recommendations to reduce the incidence of osteoporotic hip fractures...
  86. pmc Mammary epithelial cell transformation is associated with deregulation of the vitamin D pathway
    Carly M Kemmis
    Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Cell Biochem 105:980-8. 2008
    ..These studies provide evidence that disruption of the vitamin D signaling pathway occurs early in the cancer development process...
  87. ncbi A 24-phenylsulfone analog of vitamin D inhibits 1alpha,25-dihydroxyvitamin D(3) degradation in vitamin D metabolism-competent cells
    Daniel Lechner
    Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Pharmacol Exp Ther 320:1119-26. 2007
    ..Yet, S-4a imparts antimitotic activity to 1alpha,25-(OH)(2)D(3) and may therefore constitute a therapeutic to stimulate the antiproliferative potential of vitamin D-based antitumor activity...
  88. pmc Pseudovitamin D deficiency rickets--a report from the Indian subcontinent
    A H Zargar
    Department of Endocrinology, Sheri Kashmir Institute of Medical Sciences, Srinagar, Kashmir, India
    Postgrad Med J 76:369-72. 2000
    ..All patients had grossly raised 25-hydroxyvitamin D concentrations and markedly low to undetectable concentrations of 1,25-dihydroxyvitamin D. A disturbing feature of this study was the late referral of the patients...
  89. ncbi Human 25-hydroxyvitamin D-1alpha-hydroxylase: cloning, mutations, and gene expression
    A A Portale
    Department of Pediatrics, University of California, San Francisco 94143 0748, USA
    Pediatr Nephrol 14:620-5. 2000
    ..Transcriptional activity of the mouse and human P450c1alpha gene promoters can be stimulated by PTH, cAMP, and forskolin and suppressed by 1,25(OH)2D...
  90. ncbi Evidence for auto/paracrine actions of vitamin D in bone: 1alpha-hydroxylase expression and activity in human bone cells
    M van Driel
    Department of Internal Medicine, Erasmus MC, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    FASEB J 20:2417-9. 2006
    ..This is strongly dependent on osteoclast inducer RANKL. This study showing expression, activity, and functionality of 1alpha-hydroxylase unequivocally demonstrates that vitamin D can act in an auto/paracrine manner in bone...
  91. pmc Serum metabolite profiles and target tissue gene expression define the effect of cholecalciferol intake on calcium metabolism in rats and mice
    James C Fleet
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    J Nutr 138:1114-20. 2008
    ..Our data show that modeling human VD3 status requires lower intake than the current NRC rodent requirement (1000-IU/kg diet). Also, although rodents are very tolerant of high-VD3 intake, it cannot compensate for moderate Ca restriction...
  92. pmc Vitamin D and the immune system: new perspectives on an old theme
    Martin Hewison
    Department of Orthopaedic Surgery, Molecular Biology Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Endocrinol Metab Clin North Am 39:365-79, table of contents. 2010
    ..This article describes some of the recent developments with respect to vitamin D and the immune system, and possible clinical implications...
  93. pmc 20,23-dihydroxyvitamin D3, novel P450scc product, stimulates differentiation and inhibits proliferation and NF-kappaB activity in human keratinocytes
    Zorica Janjetovic
    Department for Pathology and Laboratory Medicine, Cancer Research Center, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Cell Physiol 223:36-48. 2010
    ..In summary, we have characterized 20,23(OH)2D3 as a novel secosteroidal regulator of keratinocytes proliferation and differentiation and a modifier of their immune activity...
  94. ncbi Inhibition of proliferation and induction of apoptosis by 25-hydroxyvitamin D3-3beta-(2)-Bromoacetate, a nontoxic and vitamin D receptor-alkylating analog of 25-hydroxyvitamin D3 in prostate cancer cells
    Narasimha Swamy
    Endocrinology, Diabetes and Nutrition, Department of Medicine, Boston University School of Medicine, 85 East Newton Street, Boston, MA 02118, USA
    Clin Cancer Res 10:8018-27. 2004
    ..Collectively, these results strongly suggested that 25-OH-D(3)-3-BE has a strong potential as a therapeutic agent for androgen-sensitive and androgen-refractory prostate cancer...
  95. ncbi A systematic review of the association between common single nucleotide polymorphisms and 25-hydroxyvitamin D concentrations
    John J McGrath
    Queensland Centre for Mental Health Research, The Park Centre for Mental Health, Locked Bag 500, Richlands Q4077, Australia
    J Steroid Biochem Mol Biol 121:471-7. 2010
    ..Further research is required in order to clarify the genetic architecture underlying 25OHD serum concentrations, and to unravel the mechanisms of action responsible for these associations...
  96. ncbi Modulation of vitamin D synthesis and catabolism in colorectal mucosa: a new target for cancer prevention
    Heide S Cross
    Department of Pathophysiology, Medical University of Vienna, A 1090 Vienna, Austria
    Anticancer Res 29:3705-12. 2009
    ..This could result in prevention of hyperproliferation or retardation of progression to clinically manifest primary colonic tumors...
  97. ncbi Regulation of the 5'-flanking region of the human CYP27B1 gene in osteoblast cells
    A G Turner
    Department of Medicine, Austin Health, University of Melbourne, Australia
    Mol Cell Endocrinol 311:55-61. 2009
    ..We conclude that repression of CYP27B1 gene expression may be a common event but the novel inhibitory elements we have identified may be unique to osteoblasts...
  98. pmc Genetic evidence that the human CYP2R1 enzyme is a key vitamin D 25-hydroxylase
    Jeffrey B Cheng
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 101:7711-5. 2004
    ..These data identify CYP2R1 as a biologically relevant vitamin D 25-hydroxylase and reveal the molecular basis of a human genetic disease, selective 25-hydroxyvitamin D deficiency...
  99. ncbi Regulation of gene expression by the CYP27B1 promoter-study of a transgenic mouse model
    Ivanka Hendrix
    Hanson Institute, Adelaide 5005, Australia
    J Steroid Biochem Mol Biol 89:139-42. 2004
    ..5kb, human CYP27B1 promoter. This animal model allows us to study in vivo the tissue-specific, CYP27B1 promoter-controlled, regulation of the expression of the CYP27B1 gene...
  100. ncbi 1alpha-Hydroxylase gene ablation and Pi supplementation inhibit renal calcification in mice homozygous for the disrupted Npt2a gene
    Harriet S Tenenhouse
    McGill Univ Montreal Children s Hospital Research Institute, 4060 Ste Cathe ine St West, Montreal, Quebec, Canada H3Z 2Z3
    Am J Physiol Renal Physiol 286:F675-81. 2004
    ....
  101. ncbi Sequence variants in the human 25-hydroxyvitamin D3 1-alpha-hydroxylase (CYP27B1) gene are not associated with prostate cancer risk
    Gregory A Hawkins
    Center for Human Genomics, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Prostate 53:175-8. 2002
    ..25-hydroxyvitamin D(3) 1-alpha-hydroxylase, the enzyme that catalyzes the final step of vitamin D synthesis, converting 25-hydroxyvitamin D(3) to 1,25-dihydroxyvitamin D(3), is expressed in the prostate...

Research Grants22

  1. Biology of a Novel Phosphaturic Protein - sFRP-4
    Rajiv Kumar; Fiscal Year: 2007
    ..Our findings will have important implications with respect to Pi homeostasis, bone mineralization in mammals and humans, and the regulation of ion transport through Wnt signaling in the kidney. ..
  2. The FIAT-ATF4 interaction and the control of bone mass
    Rene St Arnaud; Fiscal Year: 2010
    ..These experiments will elucidate the molecular mechanisms through which FIAT regulates bone mass accrual in vivo. ..
  3. Advances in Mineral Metabolism (AIMM) annual meeting
    Rene St Arnaud; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  4. Advances in Mineral Metabolism (AMM) meeting
    Rene St Arnaud; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  5. 2005 Bones & Teeth Gordon Conference
    Rene St Arnaud; Fiscal Year: 2005
    ..These funds, if granted, will aid immeasurably in allowing the Gordon Research Conference to fulfill its function to stimulate further creativity in bone, cartilage and tooth research. ..
  6. Pathophysiologic Regulation of Fgf-23 in Phosphate Homeostasis: Role of Vitamin D
    Beate Lanske; Fiscal Year: 2010
    ....
  7. Cardiovascular Disease Following Hypertensive Pregnancy
    Myles Wolf; Fiscal Year: 2007
    ..They believe the results will provide critical insight into mechanisms of CVD in women and potentially suggest means to alter their CVD risk. [unreadable] [unreadable]..
  8. Vitamin D and Barrier Function
    Martin Hewison; Fiscal Year: 2008
    ..abstract_text> ..
  9. Vitamin D Status and Prostate Cancer
    JAMES FLEET; Fiscal Year: 2008
    ..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
  10. Role of Indian hedgehog in Endochondral Bone Formation
    Beate Lanske; Fiscal Year: 2009
    ..Such in vivo molecular studies, could, therefore, lead to the discovery of other human skeletal diseases involving the Ihh gene and to novel therapeutic strategies aimed at modulating skeletal anomalies. ..
  11. REGULATION OF INTESTINAL CALCIUM TRANSPORT BY VITAMIN D
    Daniel Bikle; Fiscal Year: 2001
    ..These manipulations will all be tested for their ability to block 1,25D-stimulated calcium transport. ..
  12. VITAMIN D P450 FUNCTION--NATURAL & DIRECTED MUTAGENESIS
    John Omdahl; Fiscal Year: 2002
    ....
  13. Biological Measures and Hemodialysis Mortality
    Ravi Thadhani; Fiscal Year: 2006
    ..We have in place detailed plans for the protection of patient privacy, inclusion of all patients, and data/sample sharing with the larger research community. ..
  14. Ergocalciferol in ESRD Efficacy Safety and Biology
    Ravi Thadhani; Fiscal Year: 2010
    ..Our study is designed to determine safe and effective ways to raise vitamin D levels while monitoring effects on the immune system. ..
  15. Vitamin D and Cardiovascular Disease
    Ravi Thadhani; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  16. VITAMIN D AND THE DIFFERENTIATION OF KERATINOCYTES
    Daniel Bikle; Fiscal Year: 2003
    ..abstract_text> ..
  17. Colon-specific Transgenic Mouse for Cancer Research
    JAMES FLEET; Fiscal Year: 2008
    ..In addition, colon cancer models made with the new transgenic mouse will be useful for testing agents for their ability to treat and prevent cancer. [unreadable] [unreadable] [unreadable] [unreadable]..
  18. BIO-ACTIVATION OF VITAMIN D IN MAMMARY GLAND
    JoEllen Welsh; Fiscal Year: 2004
    ..These studies will provide important information regarding the expression and function of the vitamin D signaling pathway in normal mammary gland, which might have implications for breast cancer prevention. ..
  19. ANDROGENS, LIPIDS, INSULIN AND HYPERTENSIVE PREGNANCIES
    Ravi Thadhani; Fiscal Year: 2005
    ..abstract_text> ..
  20. Gestational Diabetes and Preeclampsia Cytokine Profiles
    Ravi Thadhani; Fiscal Year: 2007
    ..abstract_text> ..
  21. MOLECULAR REGULATION OF 1,25(OH)2D PRODUCTION
    Anthony Portale; Fiscal Year: 2002
    ....
  22. Antibody Microarray Predicting Pima Diabetic Nephropathy
    Ravi Thadhani; Fiscal Year: 2005
    ..This R21 proposal may provide insight into the pathogenesis of DN, provide useful biomarkers to predict DN, and suggest early targets for intervention for this devastating disorder. ..