cytochrome c group

Summary

Summary: A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)

Top Publications

  1. ncbi Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis
    H Li
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 94:491-501. 1998
  2. ncbi Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
    P Li
    Howard Hughes Medical Institute, and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 91:479-89. 1997
  3. ncbi Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition
    C Du
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 102:33-42. 2000
  4. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
  5. ncbi Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors
    X Luo
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 94:481-90. 1998
  6. ncbi An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9
    H Zou
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Biol Chem 274:11549-56. 1999
  7. pmc Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner
    E A Slee
    Molecular Cell Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
    J Cell Biol 144:281-92. 1999
  8. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
  9. ncbi Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
    X Liu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cell 86:147-57. 1996
  10. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999

Detail Information

Publications253 found, 100 shown here

  1. ncbi Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis
    H Li
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 94:491-501. 1998
    ..Coexpression of BclxL inhibits all the apoptotic changes induced by tBID. Our results indicate that BID is a mediator of mitochondrial damage induced by Casp8...
  2. ncbi Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
    P Li
    Howard Hughes Medical Institute, and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 91:479-89. 1997
    ....
  3. ncbi Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition
    C Du
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 102:33-42. 2000
    ..Overexpression of Smac increases cells' sensitivity to apoptotic stimuli. Smac is the second mitochondrial protein, along with cytochrome c, that promotes apoptosis by activating caspases...
  4. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
    ..These results indicate that AIF is a mitochondrial effector of apoptotic cell death...
  5. ncbi Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors
    X Luo
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 94:481-90. 1998
    ..The cytochrome c releasing activity of Bid was antagonized by Bcl2. A mutation at the BH3 domain diminished its cytochrome c releasing activity. Bid, therefore, relays an apoptotic signal from the cell surface to mitochondria...
  6. ncbi An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9
    H Zou
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Biol Chem 274:11549-56. 1999
    ..The stoichiometric ratio of procaspase-9 to APAF-1 is approximately 1 to 1 in the complex. Once activated, caspase-9 disassociates from the complex and becomes available to cleave and activate downstream caspases such as caspase-3...
  7. pmc Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner
    E A Slee
    Molecular Cell Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
    J Cell Biol 144:281-92. 1999
    ..Caspase-3 is required for the activation of four other caspases (-2, -6, -8, and -10) in this pathway and also participates in a feedback amplification loop involving caspase-9...
  8. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  9. ncbi Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
    X Liu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cell 86:147-57. 1996
    ..Cells undergoing apoptosis in vivo showed increased release of cytochrome c to their cytosol, suggesting that mitochondria may function in apoptosis by releasing cytochrome c...
  10. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..Our results indicate that the Bcl-2 family of proteins bind to the VDAC in order to regulate the mitochondrial membrane potential and the release of cytochrome c during apoptosis...
  11. ncbi Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3
    H Zou
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 90:405-13. 1997
    ..The COOH-terminal region of Apaf-1 comprises multiple WD repeats, which are proposed to mediate protein-protein interactions. Cytochrome c binds to Apaf-1, an event that may trigger the activation of caspase-3, leading to apoptosis...
  12. pmc Bax directly induces release of cytochrome c from isolated mitochondria
    J M Jurgensmeier
    Program on Apoptosis and Cell Death Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 95:4997-5002. 1998
    ....
  13. ncbi Dominant-negative c-Jun promotes neuronal survival by reducing BIM expression and inhibiting mitochondrial cytochrome c release
    J Whitfield
    Eisai London Research Laboratories, Bernard Katz Building, University College London, Gower Street, WC1E 6BT, London, United Kingdom
    Neuron 29:629-43. 2001
    ....
  14. ncbi Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization
    S M Srinivasula
    Center for Apoptosis Research, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Mol Cell 1:949-57. 1998
    ..Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7...
  15. ncbi Regulation of cell death protease caspase-9 by phosphorylation
    M H Cardone
    Program on Apoptosis and Cell Death Research, The Burnham Institute, La Jolla, CA 92037, USA
    Science 282:1318-21. 1998
    ..Mutant pro-Casp9(Ser196Ala) was resistant to Akt-mediated phosphorylation and inhibition in vitro and in cells, resulting in Akt-resistant induction of apoptosis. Thus, caspases can be directly regulated by protein phosphorylation...
  16. ncbi NF-kappaB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation
    C Y Wang
    Department of Endodontics, School of Dentistry, Lineberger Comprehensive Cancer Center, and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Science 281:1680-3. 1998
    ..Thus, NF-kappaB activates a group of gene products that function cooperatively at the earliest checkpoint to suppress TNF-alpha-mediated apoptosis and that function more distally to suppress genotoxic agent-mediated apoptosis...
  17. pmc Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes
    Liang Shi
    Pacific Northwest National Laboratory, Richland, Washington 99354, USA
    Mol Microbiol 65:12-20. 2007
    ..Thus, multihaem c-Cyts play critical roles in S. oneidensis MR-1- and G. sulfurreducens-mediated dissimilatory reduction of solid metal (hydr)oxides by facilitating ET across the bacterial cell envelope...
  18. ncbi Endonuclease G is an apoptotic DNase when released from mitochondria
    L Y Li
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75390, USA
    Nature 412:95-9. 2001
    ..Once released from mitochondria, endoG cleaves chromatin DNA into nucleosomal fragments independently of caspases. Therefore, endoG represents a caspase-independent apoptotic pathway initiated from the mitochondria...
  19. ncbi Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: the impact of irreversible permeability transition
    P X Petit
    CNRS UPR420, Génétique Moléculaire et Biologie du Développement 19, Villejuif, France
    FEBS Lett 426:111-6. 1998
    ..Moreover, they confirm the hypothesis formulated by Skulachev [FEBS Lett. 397 (1996) 7-10 and Q. Rev. Biophys. 29 (1996) 169-2021 linking permeability transition to activation of the apoptogenic catabolic enzymes...
  20. ncbi Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation
    Devrim Acehan
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA
    Mol Cell 9:423-32. 2002
    ..This complex promotes the efficient activation of procaspase-3. Therefore, the cleavage of procaspase-9 is not required to form an active cell death complex...
  21. ncbi Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria
    M G Vander Heiden
    Gwen Knapp Center and Committee on Immunology, Department of Medicine, University of Chicago, Illinois 60637, USA
    Cell 91:627-37. 1997
    ..These data suggest that Bcl-xL promotes cell survival by regulating the electrical and osmotic homeostasis of mitochondria...
  22. pmc Global transcriptome analysis of Shewanella oneidensis MR-1 exposed to different terminal electron acceptors
    A S Beliaev
    Biological Sciences Division, Pacific Northwest National Laboratory, P O Box 999, MS P7 50, Richland, Washington 99352, USA
    J Bacteriol 187:7138-45. 2005
    ..Moreover, the relative paucity and/or the constitutive nature of genes involved in electron transfer to metals is likely due to the low-specificity and the opportunistic nature of the metal-reducing electron transport pathways...
  23. ncbi Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis
    G V Putcha
    Departments of Neurology and Molecular Biology and Pharmacology, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Neuron 29:615-28. 2001
    ....
  24. ncbi Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c
    S J Korsmeyer
    Department of Pathology, Harvard Medical School, Dana Farber Cancer Institute, Howard Hughes Medical Institute, Boston, Massachusetts, MA 02115, USA
    Cell Death Differ 7:1166-73. 2000
    ..Thus, an activation cascade of pro-apoptotic proteins from BID to BAK or BAX integrates the pathway from surface death receptors to the irreversible efflux of cytochrome c. Cell Death and Differentiation (2000) 7, 1166 - 1173..
  25. pmc Cytochrome c biogenesis: mechanisms for covalent modifications and trafficking of heme and for heme-iron redox control
    Robert G Kranz
    Department of Biology, Washington University, One Brookings Dr, St Louis, MO 63130, USA
    Microbiol Mol Biol Rev 73:510-28, Table of Contents. 2009
    ..Step 2 includes the CcmFH-mediated reduction (to Fe(+2)) of holoCcmE and ligation of the heme to CXXCH. The evolutionary and ecological advantages for each system are discussed with respect to iron limitation and oxidizing environments...
  26. pmc tBID, a membrane-targeted death ligand, oligomerizes BAK to release cytochrome c
    M C Wei
    Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    Genes Dev 14:2060-71. 2000
    ..Activated tBID results in an allosteric activation of BAK, inducing its intramembranous oligomerization into a proposed pore for cytochrome c efflux, integrating the pathway from death receptors to cell demise...
  27. ncbi The roles of CymA in support of the respiratory flexibility of Shewanella oneidensis MR-1
    Sophie J Marritt
    Centre for Molecular and Structural Biochemistry, School of Chemistry and School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
    Biochem Soc Trans 40:1217-21. 2012
    ..This serves to highlight several unanswered questions relating to the regulation of respiratory electron transport in Shewanella and the central role(s) of the tetrahaem-containing quinol dehydrogenase CymA in that process...
  28. pmc Electrochemical measurement of electron transfer kinetics by Shewanella oneidensis MR-1
    Daniel Baron
    Biotechnology Institute, University of Minnesota, St Paul, Minnesota 55108, USA
    J Biol Chem 284:28865-73. 2009
    ..oneidensis electron transfer strategies...
  29. pmc Bax oligomerization is required for channel-forming activity in liposomes and to trigger cytochrome c release from mitochondria
    B Antonsson
    Serono Pharmaceutical Research Institute, Ares Serono International S A, 14, Chemin des Aulx, CH 1228 Plan les Ouates, Geneva, Switzerland
    Biochem J 345:271-8. 2000
    ..These results show that oligomeric Bax possesses channel-forming activity whereas monomeric Bax has no such activity...
  30. pmc Akt regulates cell survival and apoptosis at a postmitochondrial level
    H Zhou
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    J Cell Biol 151:483-94. 2000
    ..Taken together, these data suggest that Akt inhibits activation of caspase-9 and -3 by posttranslational modification of a cytosolic factor downstream of cytochrome c and before activation of caspase-9...
  31. pmc Characterization of an electron conduit between bacteria and the extracellular environment
    Robert S Hartshorne
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Proc Natl Acad Sci U S A 106:22169-74. 2009
    ..We have identified the MtrAB module in a range of bacterial phyla, suggesting that it is widely used in electron exchange with the extracellular environment...
  32. pmc Cytochrome c maintains mitochondrial transmembrane potential and ATP generation after outer mitochondrial membrane permeabilization during the apoptotic process
    N J Waterhouse
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Cell Biol 153:319-28. 2001
    ....
  33. ncbi Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis
    E A Slee
    Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College, Dublin 2, Ireland
    J Biol Chem 276:7320-6. 2001
    ....
  34. pmc Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia
    S Krajewski
    The Burnham Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 96:5752-7. 1999
    ..Loss of mitochondrial barrier function during neuronal damage from ischemia or other insults therefore may play an important role in making certain caspases available to participate in apoptosis...
  35. ncbi Reduction of nitrate in Shewanella oneidensis depends on atypical NAP and NRF systems with NapB as a preferred electron transport protein from CymA to NapA
    Haichun Gao
    College of Life Sciences and Institute of Microbiology, Zhejiang University, Hangzhou, Zhejiang, China
    ISME J 3:966-76. 2009
    ..On the basis results from mutational analyses of napA, napB, nrfA and napBnrfA in-frame deletion mutants, we propose that NapB is able to favor nitrate reduction by routing electrons to NapA exclusively...
  36. pmc Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1
    Daniel E Ross
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Appl Environ Microbiol 73:5797-808. 2007
    ..Consistent with copurification results, MtrB cross-links with MtrA, forming high-molecular-mass bands of approximately 151 and 136 kDa...
  37. pmc Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1
    Liang Shi
    Microbiology Group, Pacific Northwest National Laboratory, 902 Battelle Blvd, P O Box 999, MSIN P7 50, Richland, WA 99354, USA
    J Bacteriol 188:4705-14. 2006
    ..oneidensis MR-1...
  38. ncbi Apoptosis in the absence of caspase 3
    Y Liang
    Pediatric Center for Neuroscience, Children's Hospital of Pittsburgh, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Oncogene 20:6570-8. 2001
    ..P21 is detected only after activation of caspase 7, and P53 is neither expressed at baseline nor up-regulated with apoptosis induction. This pathway bypasses the need for activated caspase 3 in these cells...
  39. ncbi Apoptosis initiated by Bcl-2-regulated caspase activation independently of the cytochrome c/Apaf-1/caspase-9 apoptosome
    Vanessa S Marsden
    The Walter and Eliza Hall Institute, Melbourne, 3050, Australia
    Nature 419:634-7. 2002
    ..We conclude that Bcl-2 regulates a caspase activation programme independently of the cytochrome c/Apaf-1/caspase-9 'apoptosome', which seems to amplify rather than initiate the caspase cascade...
  40. pmc Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, Osaka 565-0871, Japan
    J Cell Biol 152:237-50. 2001
    ..Taken together, our data provide evidence that the VDAC plays an essential role in apoptogenic cytochrome c release and apoptosis in mammalian cells...
  41. ncbi A functional description of CymA, an electron-transfer hub supporting anaerobic respiratory flexibility in Shewanella
    Sophie J Marritt
    Centre for Molecular and Structural Biochemistry, School of Chemistry and School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U K
    Biochem J 444:465-74. 2012
    ..The implications for CymA supporting the respiratory flexibility of shewanellae are discussed...
  42. pmc Menaquinone-7 is specific cofactor in tetraheme quinol dehydrogenase CymA
    Duncan G G McMillan
    Institute of Membrane and Systems Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Biol Chem 287:14215-25. 2012
    ..However, the instability and reactivity of reaction intermediate, semiquinone, might require a cofactor that functions to minimize damaging side reactions...
  43. ncbi Sphingosine generation, cytochrome c release, and activation of caspase-7 in doxorubicin-induced apoptosis of MCF7 breast adenocarcinoma cells
    O Cuvillier
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Cell Death Differ 8:162-71. 2001
    ..Our results suggest that a further metabolite of ceramide, sphingosine, may also be involved in mitochondria-mediated apoptotic signaling induced by doxorubicin in human breast cancer cells...
  44. ncbi Rapid kinetics of tBid-induced cytochrome c and Smac/DIABLO release and mitochondrial depolarization
    Muniswamy Madesh
    Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 277:5651-9. 2002
    ..The tBid-induced loss of DeltaPsi(m) occurs after cytochrome c release and reflects impairment of oxidative metabolism...
  45. ncbi Insight into molecular stability and physiological properties of the diheme cytochrome CYC41 from the acidophilic bacterium Acidithiobacillus ferrooxidans
    Guillaume Malarte
    Bioenergetique et Ingenierie des Proteines, CNRS, IBSM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochemistry 44:6471-81. 2005
    ..A docking analysis of CYC(41), RCy, and cytochrome oxidase allowed us to propose a model for the complex in agreement with our experimental data...
  46. pmc Extracellular reduction of hexavalent chromium by cytochromes MtrC and OmcA of Shewanella oneidensis MR-1
    Sara M Belchik
    Microbiology Group, Pacific Northwest National Laboratory, 902 Battelle Blvd, MSIN J4 18, Richland, WA 99352, USA
    Appl Environ Microbiol 77:4035-41. 2011
    ..3 ± 7.9 μM, respectively. Together, these results consistently demonstrate that MtrC and OmcA are the terminal reductases used by S. oneidensis MR-1 for extracellular Cr(VI) reduction where OmcA is a predominant Cr(VI) reductase...
  47. ncbi Isolation and sequence of omcA, a gene encoding a decaheme outer membrane cytochrome c of Shewanella putrefaciens MR-1, and detection of omcA homologs in other strains of S. putrefaciens
    J M Myers
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1373:237-51. 1998
    ..In contrast, strains MR-30 and MR-42 tested negative for omcA homologs by Southern and Northern blots, RT-PCR, and Western blots...
  48. ncbi Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis
    C Kohler
    Institute of Environmental Medicine, Division of Toxicology, Karolinska Institutet, Stockholm, Sweden
    FEBS Lett 447:10-2. 1999
    ..Thus, our data suggest that only intermembrane proteins are released from mitochondria during the early phase of the apoptotic process...
  49. ncbi VDAC regulation by the Bcl-2 family of proteins
    Y Tsujimoto
    Department of Medical Genetics, Biomedical Research Center, Osaka University Graduate School of Medicine, CREST of Japan Science and Technology Corp JST, Suita, Osaka, Japan
    Cell Death Differ 7:1174-81. 2000
    ..Cell Death and Differentiation (2000) 7, 1174 - 1181..
  50. ncbi Mind the gap: cytochrome interactions reveal electron pathways across the periplasm of Shewanella oneidensis MR-1
    Bruno M Fonseca
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2780 157 Oeiras, Portugal
    Biochem J 449:101-8. 2013
    ..This reveals the co-existence of two non-mixing redox pathways that lead to extracellular electron transfer in S. oneidensis MR-1 established through transient protein interactions...
  51. ncbi Definition of the interaction domain for cytochrome c on cytochrome c oxidase. III. Prediction of the docked complex by a complete, systematic search
    V A Roberts
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 274:38051-60. 1999
    ..Electrostatic forces not only enhance long range protein/protein association; they also predominate in short range alignment, creating the transient interaction needed for rapid turnover...
  52. pmc A sulfite respiration pathway from Thermus thermophilus and the key role of newly identified cytochrome c₅₅₀
    Sylvain Robin
    Chemical and Environmental Science Department, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland
    J Bacteriol 193:3988-97. 2011
    ..The description of the complete pathway reveals that electrons generated during sulfite oxidation by the SOR are funneled into the respiratory chain, participating in the energy production of T. thermophilus...
  53. pmc The Escherichia coli CcmG protein fulfils a specific role in cytochrome c assembly
    E Reid
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, U K
    Biochem J 355:51-8. 2001
    ..The data support a model proposed by others in which CcmG catalyses one of the last reactions specific to cytochrome c assembly...
  54. ncbi Dissimilatory iron reduction in Escherichia coli: identification of CymA of Shewanella oneidensis and NapC of E. coli as ferric reductases
    Johannes S Gescher
    Department of Chemical Engineering, Stanford University, Stanford, CA, USA
    Mol Microbiol 68:706-19. 2008
    ....
  55. ncbi The cbaAB genes for bo3-type cytochrome c oxidase in Bacillus stearothermophilus
    K Nikaido
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, 680 4 Kawazu, Iizuka, Fukuoka 820 8502, Japan
    Biochim Biophys Acta 1397:262-7. 1998
    ..They are the first genes for a SoxB-type cytochrome c oxidase whose natural substrate is known...
  56. ncbi The mechanism of mitochondrial membrane potential retention following release of cytochrome c in apoptotic GT1-7 neural cells
    A C Rego
    Buck Institute for Age Research, Novato, CA 94945, USA
    Cell Death Differ 8:995-1003. 2001
    ..These results indicate that the maintenance of Delta Psi(M) following release of cyt c may be a consequence of ATP synthase reversal and cytoplasmic ATP hydrolysis in STS-treated GT1-7 cells...
  57. ncbi Bcl-xL promotes the open configuration of the voltage-dependent anion channel and metabolite passage through the outer mitochondrial membrane
    M G Vander Heiden
    Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 276:19414-9. 2001
    ..Together these results demonstrate that following an apoptotic signal, Bcl-x(L) can maintain metabolite exchange across the outer mitochondrial membrane by inhibiting VDAC closure...
  58. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
    ..Thus, activation of a "multidomain" proapoptotic member, BAX or BAK, appears to be an essential gateway to mitochondrial dysfunction required for cell death in response to diverse stimuli...
  59. pmc IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 17:2215-23. 1998
    ..These findings demonstrate that IAPs can suppress different apoptotic pathways by inhibiting distinct caspases and identify pro-caspase-9 as a new target for IAP-mediated inhibition of apoptosis...
  60. pmc The diheme cytochrome c(4) from Vibrio cholerae is a natural electron donor to the respiratory cbb(3) oxygen reductase
    Hsin Yang Chang
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    Biochemistry 49:7494-503. 2010
    ....
  61. pmc Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c
    Christian Lange
    Max Planck Institute for Biophysics, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 99:2800-5. 2002
    ..Obviously, cytochrome c reduction by the cytochrome bc1 complex can be regulated in response to respiratory conditions...
  62. pmc Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane
    R Eskes
    Serono Pharmaceutical Research Institute, Ares Serono International S A, CH 1228 Plan les Ouates, Geneva, Switzerland
    Mol Cell Biol 20:929-35. 2000
    ..Bax mitochondrial membrane insertion triggered by Bid may represent a key step in pathways leading to apoptosis...
  63. ncbi The fly caspases
    S Kumar
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    Cell Death Differ 7:1039-44. 2000
    ..The presence of multiple caspases in Drosophila indicates that apoptotic pathways in insects are likely to be as complex as in vertebrates...
  64. ncbi Hsp27 negatively regulates cell death by interacting with cytochrome c
    J M Bruey
    Inserm U 517, Faculty of Medicine and Pharmacy, 7 Boulevard Jeanne d Arc, 21033 Dijon, France
    Nat Cell Biol 2:645-52. 2000
    ..Thus, Hsp27 interferes specifically with the mitochondrial pathway of caspase-dependent cell death...
  65. pmc Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis
    S Desagher
    Serono Pharmaceutical Research Institute, Ares Serono International S A, CH 1228 Plan les Ouates, Geneva, Switzerland
    J Cell Biol 144:891-901. 1999
    ..Taken together, our results suggest that, during certain types of apoptosis, Bid translocates to mitochondria and binds to Bax, leading to a change in conformation of Bax and to cytochrome c release from mitochondria...
  66. ncbi Role of cytochrome c as a stimulator of alpha-synuclein aggregation in Lewy body disease
    M Hashimoto
    Department of Neurosciences, University of California San Diego, School of Medicine, La Jolla, California 92093 0624, USA
    J Biol Chem 274:28849-52. 1999
    ....
  67. ncbi Caspase-2 acts upstream of mitochondria to promote cytochrome c release during etoposide-induced apoptosis
    John D Robertson
    Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Box 210, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:29803-9. 2002
    ..Taken together, our data indicate that caspase-2 provides an important link between etoposide-induced DNA damage and the engagement of the mitochondrial apoptotic pathway...
  68. pmc Mitochondrial release of caspase-2 and -9 during the apoptotic process
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, F 94801 Villejuif, France
    J Exp Med 189:381-94. 1999
    ....
  69. ncbi Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor
    Seong Woon Yu
    Department of Neurology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Science 297:259-63. 2002
    ..These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death...
  70. ncbi Differential requirement for caspase 9 in apoptotic pathways in vivo
    R Hakem
    Amgen Institute, Toronto, Ontario, Canada
    Cell 94:339-52. 1998
    ..Comparison of the requirement for Casp9 and Casp3 in different apoptotic settings indicates the existence of at least four different apoptotic pathways in mammalian cells...
  71. ncbi Production and preliminary characterization of a recombinant triheme cytochrome c(7) from Geobacter sulfurreducens in Escherichia coli
    Yuri Y Londer
    Biosciences Division, Argonne National Laboratory, 9700 S Cass Avenue, Argonne, IL 60439, USA
    Biochim Biophys Acta 1554:202-11. 2002
    ..The shape parameters for the recombinant cytochrome c(7) determined by small angle X-ray scattering are in good agreement with the ones calculated from a homologous cytochrome c(7) of known structure...
  72. ncbi A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c
    Qun Ren
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Biol Chem 277:7657-63. 2002
    ..We propose that CcmFH forms a bacterial heme lyase complex for the transfer of heme from CcmE to apocytochrome c...
  73. pmc Antibody recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1
    Brian H Lower
    School of Environment and Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, USA
    Appl Environ Microbiol 75:2931-5. 2009
    ..OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance...
  74. pmc c-Type cytochrome-dependent formation of U(IV) nanoparticles by Shewanella oneidensis
    Matthew J Marshall
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America
    PLoS Biol 4:e268. 2006
    ..In the environment, such association of UO(2) nanoparticles with biopolymers may exert a strong influence on subsequent behavior including susceptibility to oxidation by O(2) or transport in soils and sediments...
  75. ncbi Granzyme B-mediated apoptosis proceeds predominantly through a Bcl-2-inhibitable mitochondrial pathway
    M J Pinkoski
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 276:12060-7. 2001
    ..Bcl-2 blocks the rapid destruction of targets by granzyme B by blocking mitochondrial involvement in the process...
  76. ncbi Impact of the bacterial type I cytochrome c maturation system on different biological processes
    Nicholas P Cianciotto
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Microbiol 56:1408-15. 2005
    ....
  77. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
    ..Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores...
  78. ncbi The role of multihaem cytochromes in the respiration of nitrite in Escherichia coli and Fe(III) in Shewanella oneidensis
    Thomas A Clarke
    Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem Soc Trans 36:1005-10. 2008
    ..UV-visible spectroscopy indicated that all five haems were low-spin, consistent with the presence of bis-His ligand co-ordination as found in full-length MtrA...
  79. ncbi Family of cytochrome c7-type proteins from Geobacter sulfurreducens: structure of one cytochrome c7 at 1.45 A resolution
    P Raj Pokkuluri
    Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439, USA
    Biochemistry 43:849-59. 2004
    ..The packing of the molecules in the crystal structure of PpcA suggests that the polymers have an elongated conformation and might form a "nanowire"...
  80. ncbi High-resolution three-dimensional structure of horse heart cytochrome c
    G W Bushnell
    Department of Biochemistry, University of British Columbia, Vancouver, Canada
    J Mol Biol 214:585-95. 1990
    ..The conservation of this structural feature and its close proximity to the heme iron atom strongly implicate this internal water molecule as having a functional role in the mechanism of action of cytochrome c...
  81. ncbi BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis
    E H Cheng
    Howard Hughes Medical Institute, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Mol Cell 8:705-11. 2001
    ..Thus, in mammals, BH3 domain-only molecules activate multidomain proapoptotic members to trigger a mitochondrial pathway, which both releases cytochrome c to activate caspases and initiates caspase-independent mitochondrial dysfunction...
  82. ncbi The membrane-bound tetrahaem c-type cytochrome CymA interacts directly with the soluble fumarate reductase in Shewanella
    C Schwalb
    Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    Biochem Soc Trans 30:658-62. 2002
    ..Potentiometric studies suggest that there are two pairs of haems with potentials of -175 and -261 mV and that 'CymA is an efficient electron donor for the soluble fumarate reductase, flavocytochrome c(3)...
  83. ncbi Cytochrome c: occurrence and functions
    Ivano Bertini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019 Sesto Fiorentino, Italy
    Chem Rev 106:90-115. 2006
  84. ncbi Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death
    A Gross
    Howard Hughes Medical Institute, Departments of Medicine and Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:1156-63. 1999
    ..Thus, while BID appears to be required for the release of cytochrome c in the TNF death pathway, the release of cytochrome c may not be required for cell death...
  85. ncbi The structure of flavocytochrome c sulfide dehydrogenase from a purple phototrophic bacterium
    Z W Chen
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110
    Science 266:430-2. 1994
    ..A tryptophan, threonine, or tyrosine side chain may provide a partial conduit for electron transfer to one of the heme groups located 10 angstroms from the flavin...
  86. pmc Altered cytochrome c display precedes apoptotic cell death in Drosophila
    J Varkey
    Department of Cell Biology and Neuroscience, University of Texas, Southwestern Medical Center, Dallas, Texas 75235 9039, USA
    J Cell Biol 144:701-10. 1999
    ..Our observations provide compelling validation of an early role for altered cytochrome c in PCD and suggest propagation of apoptotic physiology through reciprocal, feed-forward amplification involving cytochrome c and caspases...
  87. ncbi Apaf-1/cytochrome c-independent and Smac-dependent induction of apoptosis in multiple myeloma (MM) cells
    D Chauhan
    Jerome Lipper Multiple Myeloma Center, Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 276:24453-6. 2001
    ..Taken together, these findings demonstrate that Smac plays a functional role in mediating Dex-induced caspase-9 activation and apoptosis in MM cells...
  88. ncbi Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli
    S Gon
    Laboratoire de Chimie Bacterienne, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, BP 71, 13402 Marseille Cedex 20, France
    J Biol Chem 276:11545-51. 2001
    ..Therefore, TorC(C) directly transfers electrons to TorA, whereas TorC(N), which probably receives electrons from the menaquinone pool, is involved in both the electron transfer to TorC(C) and the binding to TorA...
  89. ncbi The electron transfer complex between cytochrome c552 and the CuA domain of the Thermus thermophilus ba3 oxidase. A combined NMR and computational approach
    Lucia Muresanu
    Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University, Marie Curie Strasse 9, D 60439 Frankfurt am Main, Germany
    J Biol Chem 281:14503-13. 2006
    ..Based on these structures, the electron transfer pathway from the heme of cytochrome c552 to the CuA center of the ba3 oxidase has been predicted...
  90. ncbi Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase. Electron entry and kinetic phases of the reaction
    Viktoria Drosou
    Molecular Genetics, Institute of Biochemistry, Johann Wolfgang Goethe Universitat, Frankfurt, Germany
    Eur J Biochem 269:2980-8. 2002
    ..For an alternative explanation to account for this phenomenon, we find no evidence for a second cytochrome c binding site on oxidase...
  91. pmc Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron(III), fumarate, and nitrate by Shewanella putrefaciens MR-1
    C R Myers
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
    J Bacteriol 179:1143-52. 1997
    ..The localization of the fumarate and iron(III) reductases to the periplasm and outer membrane of MR-1, respectively, suggests the possibility of a similar electron transfer role for CymA...
  92. ncbi Covalent structure of the diheme cytochrome subunit and amino-terminal sequence of the flavoprotein subunit of flavocytochrome c from Chromatium vinosum
    J J Van Beeumen
    Laboratory of Microbiology, State University of Ghent, Belgium
    J Biol Chem 266:12921-31. 1991
    ..K., Terpstra, P., and Hol, W. G. J. (1986) J. Mol. Biol. 187, 101-107) for nucleotide binding sites...
  93. ncbi Bcl-2 family: life-or-death switch
    Y Tsujimoto
    Department of Medical Genetics, Biomedical Research Center, Osaka University Graduate School of Medicine, CREST of Japan Science and Technology Corp JST, 2 2 Yamadaoka, Suita, Osaka, Japan
    FEBS Lett 466:6-10. 2000
    ..Here, we provide an overview of recent findings on the role of Bcl-2 family members in the life-or-death decision of a cell...
  94. ncbi Mitochondria and cell death. Mechanistic aspects and methodological issues
    P Bernardi
    CNR Unit for the Study of Biomembranes, Department of Biomedical Sciences, University of Padova, Italy
    Eur J Biochem 264:687-701. 1999
    ....
  95. ncbi Different interaction modes of two cytochrome-c oxidase soluble CuA fragments with their substrates
    Oliver Maneg
    Molekulare Genetik, Biozentrum, J W Goethe Universitat, Marie Curie Strasse 9, Frankfurt D 60439, Germany
    J Biol Chem 278:46734-40. 2003
    ..thermophilus only 0.5 charges are involved. Overall the results indicate that the soluble CuA domains are excellent models for the initial electron transfer processes in cytochrome-c oxidases...
  96. ncbi The structure of Acidithiobacillus ferrooxidans c(4)-cytochrome: a model for complex-induced electron transfer tuning
    Chantal Abergel
    Information Genomique et Structurale, UPR 2589, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 20, Marseille Cedex, France
    Structure 11:547-55. 2003
    ....
  97. ncbi Interaction between uranium and the cytochrome c3 of Desulfovibrio desulfuricans strain G20
    Rayford B Payne
    Department of Biochemistry, University of Missouri Columbia, Columbia, MO 65211, USA
    Arch Microbiol 181:398-406. 2004
    ....
  98. pmc Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, CREST of Japan Science and Technology Corporation JST, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:577-82. 2000
    ..These findings indicated the involvement of a different mechanism in BH3-only, protein-induced apoptogenic cytochrome c release...
  99. ncbi A role for mitochondrial Bak in apoptotic response to anticancer drugs
    G Q Wang
    Department of Pathology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 276:34307-17. 2001
    ..Together, our findings suggest that Bak plays a key role in the apoptotic machinery of cytochrome c release and thus in the chemoresistance of human T leukemic cells...
  100. ncbi Electron transfer and spectral alpha-band properties of the di-heme protein cytochrome c4 from Pseudomonas stutzeri
    L S Conrad
    Chemistry Department A, Technical University of Denmark, Lyngby
    Eur J Biochem 231:133-41. 1995
    ..The overall electron transfer pattern can be related to the recently determined crystal structure of the P. stutzeri cytochrome c4...
  101. pmc Lysine-91 of the tetraheme c-type cytochrome CymA is essential for quinone interaction and arsenate respiration in Shewanella sp. strain ANA-3
    Kamrun Zargar
    Department of Microbiology and Environmental Toxicology, University of California, 1156 High Street, Santa Cruz, CA 95064, USA
    Arch Microbiol 191:797-806. 2009
    ..These results suggest that Lys91 is physiologically important for arsenate respiration and support the hypothesis that CymA interacts with menaquinol resulting in the reduction of the cytochrome...

Research Grants63

  1. ANTIBODY PROBES OF CNS MITOCHONDRIAL APOPTOTIC FUNCTION
    Ronald Jemmerson; Fiscal Year: 2004
    ..The feasibility of the proposed research is supported by preliminary results and by the participation of on-site collaborators with expertise in the field of neuroscience, in particular the physiology of CNS mitochondria. ..
  2. Role of Proteases in Therapy-Induced Apoptosis
    Scott H Kaufmann; Fiscal Year: 2010
    ..Collectively, these studies are designed to improve current understanding of the biology of death ligand-induced apoptosis and provide new information that should be helpful in designing more effective cancer treatments. ..
  3. Apoptosis in Biochemistry and Structural Biology
    Yigong Shi; Fiscal Year: 2004
    ..By all criteria, this symposium promises to be a unique and one of the most exciting meetings in apoptosis in recent years. ..
  4. Structural Studies of Caspase Activation in Apoptosis
    Yigong Shi; Fiscal Year: 2010
    ..This specific aim, together with the closely related structural studies, will reveal comprehensive mechanisms that govern the activation of caspase-8 by DISC and the suppression of this process by FLIPs. ..
  5. Promotion of PDT Induced phototoxicity by bile acids
    David Kessel; Fiscal Year: 2003
    ..If the results of preliminary studies are borne out by further investigation, UDCA could offer a safe and effective means for promoting clinical PDT efficacy. ..
  6. MULTIDISCIPLINARY TRAINING IN EXPERIMENTAL HEMATOLOGY
    Scott Kaufmann; Fiscal Year: 2005
    ..This program is a natural outgrowth of existing training opportunities in hematology, but will provide better preparation for laboratory research in hematological diseases. (End of Abstract) ..
  7. Apoptotic effect of zinc in targeting prostate cancer
    Pei Feng; Fiscal Year: 2009
    ..The elucidation of the molecular basis of the cell specific response to zinc restoration in prostate cancer cells will contribute to the development of new approaches to the treatment of prostate cancer. ..
  8. Structural Biology of Intramembrane Proteolysis
    Yigong Shi; Fiscal Year: 2010
    ..This proposal seeks to understand the structures and mechanisms of these intramembrane proteases. ..
  9. HAUSP-mediated Deubiquitination of p53
    Yigong Shi; Fiscal Year: 2007
    ..4) Determination of the structures of the full-length HAUSP by itself and bound to p53. This study will reveal the regulation of HAUSP-mediated p53 deubiquitination. ..
  10. Thermodynamic Properties of NADH:Nitrate Reductase
    Michael Barber; Fiscal Year: 2005
    ..These studies will enhance our understanding of important structure-function relationships in NR and other related metalloflavoproteins. ..
  11. Structural Studies of Apoptosome Assembly in Apoptosis
    Yigong Shi; Fiscal Year: 2006
    ..This 1.4 MDa complex will be crystallized. The structure will be determined by either molecular replacement or by multiple isomorphous replacement. ..
  12. STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
    VICTOR DAVIDSON; Fiscal Year: 2008
    ..abstract_text> ..
  13. Conference on Photodynamic Therapy
    David Kessel; Fiscal Year: 2002
    ..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
  14. SMAD Proteins in TGF-Beta Signaling - structural studies
    Yigong Shi; Fiscal Year: 2008
    ..In particular, we will be able to examine the effects of the tumorigenic mutations on the assembly of an active transcriptional complex. ..
  15. Preclinical Evaluation of Bcr/Abl-Directed Tyrphostins
    Scott Kaufmann; Fiscal Year: 2007
    ..abstract_text> ..
  16. Structural/Biochemical Analysis: Apoptosis in C. elegans
    Yigong Shi; Fiscal Year: 2008
    ..CED3 forms a holoenzyme with oligomeric CED4. A CED4-CED3 complex has been reconstituted in vitro. The complex will be crystallized and its three-dimensional structure will be determined. ..
  17. Structural biololgy of tumor suppressor PP2A and its regulatory proteins
    Yigong Shi; Fiscal Year: 2010
    ..The generally excellent solution properties of the proteins and their cognate complexes make this undertaking feasible. ..
  18. Structure Function Relationship in Hemeproteins
    Syun Ru Yeh; Fiscal Year: 2005
    ..Experiments are proposed to test the functional consequences of this finding. These hemeprotein systems provide an excellent model for investigating fundamental structural properties that underlie biological reactivity. ..
  19. Tumor Necrosis Factor-Alpha Signaling in Breast Cancer
    Alakananda Basu; Fiscal Year: 2009
    ..The long-term objective is to develop strategies to intervene with these pathways to inhibit progression of breast cancer. ..
  20. SIGNAL TRANSDUCTION AND CELL DEATH REGULATION
    Alakananda Basu; Fiscal Year: 2004
    ....
  21. TOPOISOMERASE I INHIBITORS IN LEUKEMIA AND SOLID TUMORS
    Scott H Kaufmann; Fiscal Year: 2010
    ..Collectively, these studies are designed to provide new insight into the mechanism of action of topo I poisons, and identify strategies for enhancing their efficacy. ..
  22. OUTER MEMBRANE CYTOCHROMES--TOPOLOGY AND FUNCTION
    Charles Myers; Fiscal Year: 2002
    ..They will also contribute to environmental health by expanding our knowledge on the mechanisms by which manganese, iron, and various heavy metals are mobilized into water supplies. ..
  23. Human Lung Chromium Toxicity: Role of cytochrome b5
    Charles Myers; Fiscal Year: 2007
    ..They will provide a deeper understanding of the mechanisms underlying Cr(VI) toxicity in human lung, and will lead to new insights on the role of b5 in toxicant activation [unreadable] [unreadable]..
  24. Renal Cell Injury by Ischemia: a role for Bid
    Zheng Dong; Fiscal Year: 2009
    ..Finally, completion of the research will facilitate the design of genetic and pharmacological strategies to ameliorate ischemic renal failure by targeting Bid, Bax and their interaction. ..
  25. High-Content Cell-Based Screening for Modulators of Autophagy
    Xiao Ming Yin; Fiscal Year: 2007
    ..Our long term hope is that the knowledge and the materials developed through this study will be valuable for future clinical applications. [unreadable] [unreadable] [unreadable]..
  26. Novel Mechanism of Uranium Reduction Via Microbial Nanowires
    Gemma Reguera; Fiscal Year: 2011
    ....
  27. TRAIL-induced apoptosis in prostate cancer
    Christina Voelkel Johnson; Fiscal Year: 2008
    ..The toxicity and effectiveness of AdARR2PBTRAIL alone or in combination with doxorubicin will also be tested in subcutaneous xenografts in athymic mice. ..
  28. Ketosis, Vascular Inflammation and Its Therapy in Type 1 Diabetic Patients
    Sushil K Jain; Fiscal Year: 2010
    ..The long-term goal is to understand the role of ketosis in CVD and to discover a relatively low-cost dietary supplement that could be used as an adjuvant therapy for CVD prevention in type 1 diabetes. ..
  29. Afterdepolarizations and Cardiac Arrhythmias
    James N Weiss; Fiscal Year: 2010
    ..S. citizens each year. The goal is to use this information to develop novel therapies to prevent this deadly manifestation of heart disease. ..
  30. Polarity of Redox Control and Risk of Injury in the Alveolar Epithelium
    Dean P Jones; Fiscal Year: 2010
    ..The results of these proposed studies will provide an unprecedented knowledge concerning the subcellular compartmentalization of oxidative stress in the lung. ..
  31. Molecular Mechanisms Mediating NF-kB2/p100 Processing
    Shao Cong Sun; Fiscal Year: 2007
    ..abstract_text> ..
  32. Low-Level Prenatal Lead Exposure and Retinal Toxicity
    Donald Fox; Fiscal Year: 2009
    ....
  33. Implications of the ASK1/JNK pathway in ARF
    Prasad Devarajan; Fiscal Year: 2009
    ..The long term goal is to identify novel ASK1- and JNK-based pharmacologic interventions aimed at ameliorating renal cell damage and preventing renal failure secondary to ischemia-reperfusion injury. ..
  34. CHRONIC ETHANOL: MITOCHONDRIAL DYSFUNCTION AND APOPTOSIS
    LOU ANN SCISM BROWN; Fiscal Year: 2010
    ..3) Determine if strategies to relieve ER stress will rescue the alcoholic AT2 cell from its pro-apoptotic phenotype. 4) Determine if relief of ER stress will rescue AT2 cell apoptosis in the alcoholic lung during endotoxemia. ..
  35. Novel Targets for Protection of T Cells From Tumor-Induced Dysfunction
    Hannah Rabinowich; Fiscal Year: 2009
    ....
  36. Metabolic Oscillations in Heart
    JAMES N contact WEISS; Fiscal Year: 2010
    ..These insights may suggest novel therapies to protect the heart from injury during metabolic stresses such as heart attacks. ..
  37. Modified HER-2 Tumor Antigens for Vaccination in Cancer
    Mark Mamula; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  38. Combination cytokine gene therapy for melanoma treatment
    Alain Luxembourg; Fiscal Year: 2002
    ..Initial indications would metastatic melanoma patients, for whom there are for therapeutic alternatives. ..
  39. Isoaspartyl Modified Tumor Antigens for Vaccination
    Mark Mamula; Fiscal Year: 2004
    ..It is anticipated that this approach will lead to novel immunotherapies for the treatment of human tumors. ..
  40. TRAINING IN CLINICAL INVESTIGATION FOR PHYSICIANS
    Joseph Craft; Fiscal Year: 2004
    ..The curriculum takes maximum advantage of the strong training environment already in place in the Medical School. ..
  41. PATHOGENESIS OF CHLAMYDIAL OCULAR INFECTIONS
    Deborah Dean; Fiscal Year: 2004
    ..A greater understanding of the pathogenesis of trachoma will aid in developing public health interventions and a vaccine that can be implemented in trachoma endemic areas throughout the world. ..
  42. OXIDATION OF MACROMOLECULES IN SPINAL CORD INJURY
    Danxia Liu; Fiscal Year: 2001
    ..This will directly test whether free radicals damage neurons by inducing oxidation of major cell components and will provide strategies for therapy. ..
  43. ACTIVATION OF IKB KINASE BY HTLV-I TAX
    Shao Cong Sun; Fiscal Year: 2003
    ..abstract_text> ..
  44. ANKYRIN: CRUCIAL LINKER IN RENAL CELL APOPTOTIC CASCADES
    Prasad Devarajan; Fiscal Year: 2003
    ..The results will provide important insights into apoptotic cell death that accompanies ischemia and several other renal pathophysiologic states, and reveal critical clues for potential therapeutic interventions. ..
  45. Potentiating a DNA vaccine for HBV with electroporation
    Alain Luxembourg; Fiscal Year: 2003
    ..Protocols matching predefined criteria will be tested in Phase II in animal models of chronic HBV infection. ..
  46. Ionic Charge and pH Regulation of Apoptotic Signaling
    Mark Segal; Fiscal Year: 2002
    ..This grant will also enhance the research productivity, fiscal independence and career development of the applicant furthering his advancement towards becoming an independent investigator. ..
  47. Fourier Transform Ion Cyclotron Resonance Mass Spect.
    Mehdi Moini; Fiscal Year: 2003
    ..3) Chemical Composition Analysis of Expanded Porphyrins. Some of these expanded porphyrins are used to develop drugs for diagnosis and treatment of human diseases such as radiation therapy enhancing agents. ..
  48. Nipah Virus Marker Vaccine and Companion Diagnostic Test
    James Roth; Fiscal Year: 2005
    ..abstract_text> ..
  49. A new approach to xenotransplantation in primate
    Juan Contreras; Fiscal Year: 2005
    ..The research proposed in this grant will provide significant information to the xenotransplant research and the potential use of porcine islets as an alternative to human tissue for pancreatic islet transplantation. ..
  50. Mimic Peptides of HIV-1 Epitopes as Vaccines
    Darcy Wilson; Fiscal Year: 2005
    ..Ultimately, this peptide sequence information will be incorporated into a therapeutic/ prophylactic multi-epitope vaccine formulation based on multiple peptides, fusion proteins or DNA sequences in viral vectors. ..
  51. T Cell Activation in Lupus Proteomic Characterization
    Joseph Craft; Fiscal Year: 2005
    ..abstract_text> ..
  52. REGULATORY MECHANISMS IN THE IMMUNE SYSTEM
    John Kappler; Fiscal Year: 2006
    ....
  53. Bc1-2 Family Proteins in the Ischemic Neuronal Injury
    Xiao Ming Yin; Fiscal Year: 2006
    ..To characterize the molecular interactions of Bid and Bax in inducing dysfunction of mitochondria isolated from the brain. ..
  54. Apoptotic Mediators in HIV Associated Dementia
    Gwenn Garden; Fiscal Year: 2006
    ..How p53 may promote neurodegeneration through the modulation of caspase enzyme activity will also be studied. ..
  55. Development of Etched Modified Capillaries
    JOSEPH PESEK; Fiscal Year: 2002
    ..Microsolv Corporation will undertake a marketing plan to distribute the products for use by companies working in the targeted application areas. ..
  56. Development of a DNA Vaccine for Chronic HBV Infection
    Claire Evans; Fiscal Year: 2007
    ..The treatment of this condition would be much improved by a safe and effective vaccine therapy that could durably eradicate the virus. [unreadable] [unreadable] [unreadable]..
  57. Sixth International Conference on Legionella
    Nicholas Cianciotto; Fiscal Year: 2005
    ..This unique gathering of microbiologists, physicians, public health officials, and engineers is the definitive meeting for those concerned with Legionella and its associated diseases. ..
  58. Mitochondria and Cardiac Cell Death
    James Weiss; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  59. c-Myc, growth factor and breast cancer
    Dezhong Liao; Fiscal Year: 2007
    ..abstract_text> ..
  60. MOLECULAR AND CHEMICAL DESCRIPTION OF CFTR FUNCTION
    Peter Pedersen; Fiscal Year: 2003
    ..The proposed studies are fundamental to understanding structure/function relationships within CFTR, to understanding the underlying basis of most cases of CF, and to developing new strategies to treat the disease. ..
  61. PROPERTIES OF INWARD RECTIFIER K CHANNELS
    James Weiss; Fiscal Year: 2007
    ..abstract_text> ..
  62. Targeting Akt/NF-kappa beta for Pancreatic Cancer Therapy
    Fazlul Sarkar; Fiscal Year: 2007
    ..These results will provide mechanistic as well as pre-clinical data in support of our hypotheses and may open new and novel avenues for the treatment of human pancreatic cancer. ..