acyl coenzyme a

Summary

Summary: S-Acyl coenzyme A. Fatty acid coenzyme A derivatives that are involved in the biosynthesis and oxidation of fatty acids as well as in ceramide formation.

Top Publications

  1. pmc Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
    A Dahlqvist
    Scandinavian Biotechnology Research ScanBi AB, Herman Ehles Väg 2 S 26831 Svalôv, Sweden
    Proc Natl Acad Sci U S A 97:6487-92. 2000
  2. pmc The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal
    Qin Fang Chen
    School of Biological Sciences, The University of Hong Kong, Hong Kong, China
    New Phytol 186:843-55. 2010
  3. pmc Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis
    Pooja Arora
    National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Nat Chem Biol 5:166-73. 2009
  4. pmc Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics
    Rémi Peyraud
    Institute of Microbiology, Eidgenössiche Technische Hochschule Zurich, 8093 Zurich, Switzerland
    Proc Natl Acad Sci U S A 106:4846-51. 2009
  5. pmc Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry
    Christopher A Haynes
    School of Biology, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
    J Lipid Res 49:1113-25. 2008
  6. ncbi Technical Advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues
    T R Larson
    Centre for Novel Agricultural Products, Department of Biology, University of York, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Plant J 25:115-25. 2001
  7. pmc Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: diacylglycerol acyltransferase
    Michael Buszczak
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Genetics 160:1511-8. 2002
  8. pmc Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling
    N J Faergeman
    Institute of Biochemistry, Odense University, DK 5230 Odense, Denmark
    Biochem J 323:1-12. 1997
  9. ncbi Cloning and characterization of a mammalian fatty acyl-CoA elongase as a lipogenic enzyme regulated by SREBPs
    Takashi Matsuzaka
    Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki 305 8575, Japan
    J Lipid Res 43:911-20. 2002
  10. ncbi Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase
    P Bouvier-Nave
    Institut de Biologie Moleculaire des Plantes, Strasbourg, France
    Eur J Biochem 267:85-96. 2000

Research Grants

Detail Information

Publications236 found, 100 shown here

  1. pmc Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
    A Dahlqvist
    Scandinavian Biotechnology Research ScanBi AB, Herman Ehles Väg 2 S 26831 Svalôv, Sweden
    Proc Natl Acad Sci U S A 97:6487-92. 2000
    ..However, budding yeast PDAT and its relatives in fission yeast and Arabidopsis form a distinct branch within this protein superfamily, indicating that a separate PDAT enzyme arose at an early point in evolution...
  2. pmc The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal
    Qin Fang Chen
    School of Biological Sciences, The University of Hong Kong, Hong Kong, China
    New Phytol 186:843-55. 2010
    ..As recombinant ACBP1 and ACBP2 bind unsaturated phosphatidylcholine and acyl-CoA esters in vitro, we propose that ACBP1 and ACBP2 are essential in lipid transfer during early embryogenesis in Arabidopsis...
  3. pmc Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis
    Pooja Arora
    National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Nat Chem Biol 5:166-73. 2009
    ..Because FAALs in Mtb are crucial nodes in the biosynthetic network of virulent lipids, inhibitors directed against these proteins provide a unique multipronged approach to simultaneously disrupting several pathways...
  4. pmc Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics
    Rémi Peyraud
    Institute of Microbiology, Eidgenössiche Technische Hochschule Zurich, 8093 Zurich, Switzerland
    Proc Natl Acad Sci U S A 106:4846-51. 2009
    ..extorquens AM1 has major implications for the physiology of these methylotrophs and their role in nature, and it also provides a common ground for C1 and C2 compound assimilation in isocitrate lyase-negative bacteria...
  5. pmc Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry
    Christopher A Haynes
    School of Biology, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
    J Lipid Res 49:1113-25. 2008
    ..This simple and robust method should facilitate the inclusion of this family of compounds in "lipidomics" and "metabolomics" studies...
  6. ncbi Technical Advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues
    T R Larson
    Centre for Novel Agricultural Products, Department of Biology, University of York, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Plant J 25:115-25. 2001
    ....
  7. pmc Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: diacylglycerol acyltransferase
    Michael Buszczak
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Genetics 160:1511-8. 2002
    ..The midway gene encodes a protein similar to mammalian acyl coenzyme A: diacylglycerol acyltransferase (DGAT), which converts diacylglycerol (DAG) into triacylglycerol (TAG)...
  8. pmc Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling
    N J Faergeman
    Institute of Biochemistry, Odense University, DK 5230 Odense, Denmark
    Biochem J 323:1-12. 1997
    ..This view is further supported by the observation that fatty acids do not repress expression of acetyl-CoA carboxylase or Delta9-desaturase in yeast deficient in acyl-CoA synthetase...
  9. ncbi Cloning and characterization of a mammalian fatty acyl-CoA elongase as a lipogenic enzyme regulated by SREBPs
    Takashi Matsuzaka
    Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki 305 8575, Japan
    J Lipid Res 43:911-20. 2002
    ....
  10. ncbi Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase
    P Bouvier-Nave
    Institut de Biologie Moleculaire des Plantes, Strasbourg, France
    Eur J Biochem 267:85-96. 2000
    ....
  11. ncbi Studies of propionate toxicity in Salmonella enterica identify 2-methylcitrate as a potent inhibitor of cell growth
    A R Horswill
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706 1567, USA
    J Biol Chem 276:19094-101. 2001
    ..This result lent further support to the in vivo data, which suggested that citrate synthase was the source of a toxic metabolite...
  12. doi A prokaryotic acyl-CoA reductase performing reduction of fatty acyl-CoA to fatty alcohol
    Per Hofvander
    Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Alnarp, Sweden
    FEBS Lett 585:3538-43. 2011
    ..The Marinobacter fatty acyl reductase studied has a wide substrate range in comparison to what can be found among enzymes so far studied in eukaryotes...
  13. pmc Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources
    Stephane Vuilleumier
    Département Micro organismes, Génomes, Environnement, Universite de Strasbourg, UMR7156 CNRS, Strasbourg, France
    PLoS ONE 4:e5584. 2009
    ....
  14. pmc Identification of lysine succinylation as a new post-translational modification
    Zhihong Zhang
    Ben May Department for Cancer Research, The University of Chicago, Chicago, Illinois, USA
    Nat Chem Biol 7:58-63. 2011
    ..Given the apparent high abundance of lysine succinylation and the significant structural changes induced by this PTM, it is expected that lysine succinylation has important cellular functions...
  15. ncbi On the mechanism of action of the antifungal agent propionate
    Matthias Brock
    Laboratorium für Mikrobiologie, Universitat Hannover, Germany
    Eur J Biochem 271:3227-41. 2004
    ..A possible toxicity of propionyl-CoA for humans in diseases such as propionic acidaemia and methylmalonic aciduria is also discussed...
  16. pmc Mouse cardiac acyl coenzyme a synthetase 1 deficiency impairs Fatty Acid oxidation and induces cardiac hypertrophy
    Jessica M Ellis
    Department of Nutrition, CB 7461, University of North Carolina at Chapel Hill, 135 Dauer Drive, MHRC 2301, Chapel Hill, NC 27599, USA
    Mol Cell Biol 31:1252-62. 2011
    Long-chain acyl coenzyme A (acyl-CoA) synthetase isoform 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids and contributes the majority of cardiac long-chain acyl-CoA synthetase activity...
  17. pmc Long-chain acyl-CoA esters and phosphatidylinositol phosphates modulate ATP inhibition of KATP channels by the same mechanism
    Dirk Schulze
    Institute of Physiology II, Friedrich Schiller University Jena, Teichgraben 8, 07740 Jena, Germany
    J Physiol 552:357-67. 2003
    ..We provide evidence that CoA and diadenosine polyphosphates (e.g. Ap4A) are ligands of the inhibitory ATP-binding site on Kir6.2...
  18. pmc In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor
    J E Cronan
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    J Bacteriol 179:1819-23. 1997
    ..These fatty acids were found to be efficient modulators of FadR action only when the acids could be converted to acyl-CoAs...
  19. ncbi Characterization and cloning of an (R)-specific trans-2,3-enoylacyl-CoA hydratase from Rhodospirillum rubrum and use of this enzyme for PHA production in Escherichia coli
    S E Reiser
    Monsanto Company, AG Sector, St Louis, MO 63198, USA
    Appl Microbiol Biotechnol 53:209-18. 2000
    ..coli strain DH5alpha. Growth of these strains on oleic acid resulted in the production of the terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) ...
  20. doi Molecular characterization of a lysophosphatidylcholine acyltransferase gene belonging to the MBOAT family in Ricinus communis L
    José María Arroyo-Caro
    Centro de Investigación en Biotecnología Agroalimentaria, Universidad de Almeria, Campus de Excelencia Internacional Agroalimentario CEIA3, CITE II B, Almeria, Spain
    Lipids 48:663-74. 2013
    ..These characteristics are discussed in terms of a possible role of RcLPCAT in regulating the entry of FA into PtdCho and the exclusion from the membranes of the hydroxylated FA...
  21. pmc Measurement of tissue acyl-CoAs using flow-injection tandem mass spectrometry: acyl-CoA profiles in short-chain fatty acid oxidation defects
    Andrew A Palladino
    Division of Endocrinology, Department of Pediatrics, Children s Hospital of Philadelphia and Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Genet Metab 107:679-83. 2012
    ..There was more modest accumulation of medium chain species including 3-hydroxyacyl-CoA's consistent with the known chain-length specificity of the SCHAD enzyme...
  22. pmc Metabolic interactions between the Lands cycle and the Kennedy pathway of glycerolipid synthesis in Arabidopsis developing seeds
    Liping Wang
    National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada
    Plant Cell 24:4652-69. 2012
    ..Our study thus illustrates an intricate relationship between the Lands cycle and the Kennedy pathway...
  23. ncbi Long-chain acyl-coenzyme A esters and fatty acids directly link metabolism to K(ATP) channels in the heart
    G X Liu
    Institute of Physiology, Marburg University, Marburg, Germany
    Circ Res 88:918-24. 2001
    ..These findings indicate that LC fatty acids and their CoA-linked derivatives may be key physiological modulators of K(ATP) channel activity in the heart...
  24. pmc Cytoplasmic accumulation of long-chain coenzyme A esters activates KATP and inhibits Kir2.1 channels
    Ekaterina Shumilina
    Institute of Physiology II, Friedrich Schiller University, Jena, Teichgraben 8, 07743 Jena, Germany
    J Physiol 575:433-42. 2006
    Long-chain fatty acids acyl coenzyme A esters (LC-CoA) are obligate intermediates of fatty acid metabolism and have been shown to activate K(ATP) channels but to inhibit most other Kir channels (e.g. Kir2.1) by direct channel binding...
  25. doi Gene acquisition, duplication and metabolic specification: the evolution of fungal methylisocitrate lyases
    Sebastian Müller
    Leibniz Institute for Natural Product Research and Infection Biology e V, Hans Knoell Institute, Jena, Germany
    Environ Microbiol 13:1534-48. 2011
    ..Thus, gene duplication has increased the metabolic capacity of fungi...
  26. ncbi Regulation of lipolytic activity by long-chain acyl-coenzyme A in islets and adipocytes
    Liping Hu
    Obesity Research Center, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 289:E1085-92. 2005
    ..Together, these opposite actions of LC-CoA lower circulating fat by inhibiting its release from adipocytes and promoting fat storage via insulin action...
  27. ncbi Activation of adenosine triphosphate-sensitive potassium channels by acyl coenzyme A esters involves multiple phosphatidylinositol 4,5-bisphosphate-interacting residues
    Jocelyn E Manning Fox
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Mol Endocrinol 18:679-86. 2004
    ATP-sensitive potassium (K(ATP)) channels are crucial to pancreatic endocrine function and their activation by acyl coenzyme A esters (acyl CoAs) may disrupt hormone secretion, contributing to the pathophysiology of type 2 diabetes...
  28. pmc Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold
    D M van Aalten
    Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO J 19:5167-77. 2000
    FadR is a dimeric acyl coenzyme A (acyl CoA)-binding protein and transcription factor that regulates the expression of genes encoding fatty acid biosynthetic and degrading enzymes in Escherichia coli. Here, the 2...
  29. ncbi Effect of mutagenesis on the stereochemistry of enoyl-CoA hydratase
    Yuguo Feng
    Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794 3400, USA
    Biochemistry 41:12883-90. 2002
    ..Finally, we also demonstrate that hydrogen bonds in the oxyanion hole, provided by the backbone amide groups of G141 and A98, are important for the formation of both product enantiomers...
  30. ncbi Evolution of function in the crotonase superfamily: the stereochemical course of the reaction catalyzed by 2-ketocyclohexanecarboxyl-CoA hydrolase
    Ellen D Eberhard
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Am Chem Soc 126:7188-9. 2004
    ....
  31. ncbi The crystal structure of dienoyl-CoA isomerase at 1.5 A resolution reveals the importance of aspartate and glutamate sidechains for catalysis
    Y Modis
    European Molecular Biology Laboratory, Heidelberg, Germany
    Structure 6:957-70. 1998
    ..Dienoyl-CoA isomerase belongs to a family of CoA-binding proteins that share the enoyl-CoA hydratase/isomerase sequence motif...
  32. pmc Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB
    Gonzalo Durante-Rodríguez
    Department of Environmental Biology, Centro de Investigaciones Biologicas Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain
    J Biol Chem 285:35694-705. 2010
    ..A model of action of the BzdR regulator has been proposed...
  33. pmc Structural and functional characterization of a new recombinant histidine-tagged acyl coenzyme A binding protein (ACBP) from mouse
    Anca D Petrescu
    Department of Physiology and Pharmacology, Texas A and M University, 4467 TAMU, TVMC, College Station, TX 77843 4467, USA
    Protein Expr Purif 58:184-93. 2008
    b>Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism...
  34. ncbi Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 356:183-7. 1994
    ..It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids...
  35. doi Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol
    Robert M Willis
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, United States
    Biochemistry 50:10550-8. 2011
    ..The substrate cis-11-hexadecenal was reduced in a cooperative manner with an apparent K(m) of ~50 μM, V(max) of ~8 μmol NADP(+) min(-1) (mg protein)(-1), and n ~ 2...
  36. ncbi Analysis of acyl coenzyme A binding to the transcription factor FadR and identification of amino acid residues in the carboxyl terminus required for ligand binding
    N Raman
    Department of Biochemistry, University of Tennessee, Memphis 38163
    J Biol Chem 270:1092-7. 1995
    ..Previously, we demonstrated that the ability of purified FadR to bind DNA in vitro is inhibited by long chain acyl coenzyme A esters (DiRusso, D. D., Heimert, T. L., and Metzger, A. K. (1992) J. Biol. Chem. 267, 8685-8691)...
  37. ncbi Long chain coenzyme A esters activate the pore-forming subunit (Kir6. 2) of the ATP-regulated potassium channel
    R Branstrom
    Rolf Luft Center for Diabetes Research, Department of Molecular Medicine, Karolinska Institute, S 171 76 Stockholm, Sweden
    J Biol Chem 273:31395-400. 1998
    ..2 is the primary target for LC-CoA esters to activate the KATP channel and that the esters are likely to induce a conformational change by a direct interference with the pore-forming subunit, leading to openings of long duration...
  38. doi Elevation in intracellular long-chain acyl-coenzyme A esters lead to reduced beta-cell excitability via activation of adenosine 5'-triphosphate-sensitive potassium channels
    Nicola J Webster
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada
    Endocrinology 149:3679-87. 2008
    ..These results suggest a direct link between intracellular fatty ester accumulation and K(ATP) channel activation, which may contribute to beta-cell dysfunction in type 2 diabetes...
  39. pmc The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR
    D M van Aalten
    Wellcome Trust Biocentre, Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO J 20:2041-50. 2001
    ..2 A in separation of the DNA recognition helices and the loss of DNA binding, revealing the molecular basis of acyl-CoA-responsive regulation...
  40. ncbi Characterization of an acyl-CoA-binding protein from Arabidopsis thaliana
    N J Engeseth
    Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824, USA
    Arch Biochem Biophys 331:55-62. 1996
    ..Developing B. napus seeds underwent a 12-fold increase in ACBP levels during seed maturation (20 to 250 micrograms ACBP g(-1) FW); the highest concentration occurring near the peak of triacylglycerol accumulation (26 nmol g(-1) FW...
  41. doi An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members
    Shi Xiao
    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Plant Physiol Biochem 47:479-84. 2009
    ....
  42. doi Light-regulated Arabidopsis ACBP4 and ACBP5 encode cytosolic acyl-CoA-binding proteins that bind phosphatidylcholine and oleoyl-CoA ester
    Shi Xiao
    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Plant Physiol Biochem 47:926-33. 2009
    ..Nonetheless, His-tagged ACBP4 and ACBP5 resemble ACBP6 in their ability to bind phosphatidylcholine suggesting that all three ACBPs are available for the intracellular transfer of phosphatidylcholine...
  43. pmc Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil
    Julie Burgal
    Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, WA 99164 6340, USA
    Plant Biotechnol J 6:819-31. 2008
    ..Our results demonstrate that pathway engineering approaches can be used successfully to increase the yields of industrial feedstocks in plants, and that members of the DGAT2 gene family probably play a key role in this process...
  44. pmc Fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation
    Hsin Hung Chou
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS Genet 5:e1000652. 2009
    ....
  45. ncbi The DGA1 gene determines a second triglyceride synthetic pathway in yeast
    Peter Oelkers
    Institute of Human Nutrition and the Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 277:8877-81. 2002
    ..Yeast strains deficient in both diacylglycerol and sterol esterification showed only a slight growth defect indicating that neutral lipid synthesis is dispensable under common laboratory conditions...
  46. ncbi Mechanism of 8-amino-7-oxononanoate synthase: spectroscopic, kinetic, and crystallographic studies
    S P Webster
    Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, King s Buildings, Edinburgh EH9 3JJ, United Kingdom
    Biochemistry 39:516-28. 2000
    ..These results have been combined to form a detailed mechanistic pathway for AONS catalysis which may be applied to the other members of the alpha-oxoamine synthase subfamily...
  47. pmc Characterization of an archaeal medium-chain acyl coenzyme A synthetase from Methanosarcina acetivorans
    Yu Meng
    Clemson University, Department of Genetics and Biochemistry, Clemson, SC 29634 0318, USA
    J Bacteriol 192:5982-90. 2010
    Short- and medium-chain acyl coenzyme A (acyl-CoA) synthetases catalyze the formation of acyl-CoA from an acyl substrate, ATP, and CoA...
  48. pmc Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis
    Angela A Wendel
    Department of Nutrition, CB 7461, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Biochim Biophys Acta 1791:501-6. 2009
    ..Much work remains to fully delineate the regulation of each GPAT isoform and its individual role in triacylglycerol synthesis...
  49. pmc Secondary mitochondrial dysfunction in propionic aciduria: a pathogenic role for endogenous mitochondrial toxins
    Marina A Schwab
    Department of General Pediatrics, Division of Inborn Metabolic Diseases, University Children s Hospital Heidelberg, Im Neuenheimer Feld 150, D 69120 Heidelberg, Germany
    Biochem J 398:107-12. 2006
    ....
  50. pmc Recent progress on acyl CoA: lysophospholipid acyltransferase research
    Hideo Shindou
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Lipid Res 50:S46-51. 2009
    ..These discoveries have prompted a robust surge of research in this field. In this review, we focus on the cloning and characterization of lysophospholipid acyltransferases (LPLATs), which contribute to membrane asymmetry and diversity...
  51. ncbi Control of mitochondrial beta-oxidation flux
    Simon Eaton
    Surgery Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Prog Lipid Res 41:197-239. 2002
    ..The sharing of control between CPTI and other enzymes allows for flexible regulation of metabolism and the ability to rapidly adapt beta-oxidation flux to differing requirements in different tissues...
  52. pmc Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstroms resolution: a spiral fold defines the CoA-binding pocket
    C K Engel
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 15:5135-45. 1996
    ..This water molecule is bound between the two catalytic glutamates and could serve as the activated water during catalysis...
  53. pmc Glyoxylate regeneration pathway in the methylotroph Methylobacterium extorquens AM1
    Natalia Korotkova
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195 1750, USA
    J Bacteriol 184:1750-8. 2002
    ..A new scheme for the pathway is proposed based on these data...
  54. ncbi Metabolic regulation of the pancreatic beta-cell ATP-sensitive K+ channel: a pas de deux
    Andrei Tarasov
    University Laboratory of Physiology, Parks Rd, Oxford OX1 3PT, UK
    Diabetes 53:S113-22. 2004
    ..This metabolic pas de deux orchestrates the pivotal role of ATP in metabolic regulation of the KATP channel...
  55. pmc Specificity of activation by phosphoinositides determines lipid regulation of Kir channels
    Tibor Rohacs
    Department of Physiology and Biophysics, Mount Sinai School of Medicine of New York University, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 100:745-50. 2003
    ..1 channel decreasing phosphoinositide specificity allow activation by LC acyl-CoA. Our data demonstrate that differences in phosphoinositide specificity determine the modulation of Kir channel activity by distinct regulatory lipids...
  56. ncbi Long-chain CoA esters activate human pancreatic beta-cell KATP channels: potential role in Type 2 diabetes
    R Branstrom
    Rolf Luft Center for Diabetes Research, Karolinska Hospital, Karolinska Institutet, Stockholm, Sweden
    Diabetologia 47:277-83. 2004
    ..Recently, we showed that long-chain CoA (LC-CoA) esters form a new class of potent KATP channel activators in rodents, as studied in inside-out patches...
  57. pmc Acyl-CoA synthesis, lipid metabolism and lipotoxicity
    Lei O Li
    Department of Nutrition, CB 7461, University of North Carolina, Chapel Hill, NC 27599 7461, USA
    Biochim Biophys Acta 1801:246-51. 2010
    ....
  58. ncbi Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors
    Christian Riebeling
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 278:43452-9. 2003
    ..and Futerman, A. H. (2002) FEBS Lett. 528, 3-4) that Lag1p family members regulate (dihydro)ceramide synthases responsible for production of sphingolipids containing different fatty acids...
  59. pmc Propionyl-CoA and adenosylcobalamin metabolism in Caenorhabditis elegans: evidence for a role of methylmalonyl-CoA epimerase in intermediary metabolism
    Randy J Chandler
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Genet Metab 89:64-73. 2006
    ..elegans RNAi and deletion mutants...
  60. ncbi Role of acylCoA binding protein in acylCoA transport, metabolism and cell signaling
    J Knudsen
    Biokemisk Institut, Odense Universitet, Denmark
    Mol Cell Biochem 192:95-103. 1999
    ....
  61. ncbi Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism
    Matthias Mack
    Institut für Technische Mikrobiologie der Hochschule Mannheim, Germany
    FEBS J 273:2012-22. 2006
    ..The only missense mutation found in MGA1 phenotypes, c.719C>T, leading to the amino acid exchange A240V, produces an enzyme with only 9% of the wild-type 3-MG-CoA hydratase activity...
  62. ncbi Fatty acid signaling in the beta-cell and insulin secretion
    Christopher J Nolan
    Department of Endocrinology, Canberra Hospital, Australia
    Diabetes 55:S16-23. 2006
    ..Improved knowledge of lipid signaling in the beta-cell will allow a better understanding of the mechanisms of beta-cell compensation and failure in diabetes...
  63. pmc Regulation of Triglyceride Metabolism. II. Function of mitochondrial GPAT1 in the regulation of triacylglycerol biosynthesis and insulin action
    Maria R Gonzalez-Baró
    Instituto de Investigaciones Bioquímicas de La Plata CONICET UNLP, La Plata, Argentina
    Am J Physiol Gastrointest Liver Physiol 292:G1195-9. 2007
    ....
  64. ncbi Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteria
    Omita A Trivedi
    National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Nature 428:441-5. 2004
    ..Similar mechanisms may operate in the biosynthesis of other lipid-containing metabolites and could have implications in engineering novel hybrid products...
  65. doi Hereditary and acquired diseases of acyl-coenzyme A metabolism
    Grant A Mitchell
    Division of Medical Genetics, CHU Sainte Justine, 3175 Cote Sainte Catherine Road, Montreal, Que, Canada H1R 2A6
    Mol Genet Metab 94:4-15. 2008
    ....
  66. ncbi New reactions in the crotonase superfamily: structure of methylmalonyl CoA decarboxylase from Escherichia coli
    M M Benning
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    Biochemistry 39:4630-9. 2000
    ..The new constellation of putative functional groups observed in the active site of MMCD underscores the diversity of function in this superfamily...
  67. doi Acyl-CoA:lysophospholipid acyltransferases
    Hideo Shindou
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Biol Chem 284:1-5. 2009
    ..In this review, we summarize recent studies on cloning and characterization of lysophospholipid acyltransferases that contribute to membrane asymmetry and diversity...
  68. ncbi Polyunsaturated fatty acyl coenzyme A suppress the glucose-6-phosphatase promoter activity by modulating the DNA binding of hepatocyte nuclear factor 4 alpha
    Fabienne Rajas
    INSERM U 449, Faculte de Medecine Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France
    J Biol Chem 277:15736-44. 2002
    ..The binding of HNF4 alpha to its cognate sites is specifically inhibited by polyunsaturated fatty acyl coenzyme A in vitro...
  69. pmc Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Fakultat fur Chemie, Pharmazie und Geowissenschaften, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Proc Natl Acad Sci U S A 104:10631-6. 2007
    ..For methylotrophic bacteria such as Methylobacterium extorquens, extension of the serine cycle with reactions of the ethylmalonyl-CoA pathway leads to a simplified scheme for isocitrate lyase-independent C1 assimilation...
  70. ncbi Topology of acyltransferase motifs and substrate specificity and accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1
    Atsushi Yamashita
    Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Sagamihara, Kanagawa 229 0195, Japan
    Biochim Biophys Acta 1771:1202-15. 2007
    ..In contrast, short chain acyl-CoAs did access the catalytic domain but did not serve as the second substrate. These results suggest that motifs II and III are involved in LPA binding and motifs I and IV are involved in acyl-CoA binding...
  71. ncbi Long-chain acyl-CoA esters as indicators of lipid metabolism and insulin sensitivity in rat and human muscle
    B A Ellis
    Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, New South Wales, Australia
    Am J Physiol Endocrinol Metab 279:E554-60. 2000
    ....
  72. ncbi Acyl coenzyme a synthetase regulation: putative role in long-chain acyl coenzyme a partitioning
    Yan Lin Wang
    Department of Medicine, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 12:1781-8. 2004
    Long-chain acyl coenzyme A synthetase (ACSL) converts free fatty acids (FFAs) into their metabolizable long-chain acyl coenzyme A (LC-CoA) derivatives that are essential for FFA conversion to CO(2), triglycerides, or complex lipids...
  73. ncbi Characterization of the propionyl-CoA synthetase (PrpE) enzyme of Salmonella enterica: residue Lys592 is required for propionyl-AMP synthesis
    Alexander R Horswill
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Biochemistry 41:2379-87. 2002
    ..To the best of our knowledge, these mutant proteins are the first acyl-CoA synthetases reported that are defective in adenylation activity...
  74. ncbi Promiscuous fatty acyl CoA ligases produce acyl-CoA and acyl-SNAC precursors for polyketide biosynthesis
    Pooja Arora
    National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India
    J Am Chem Soc 127:9388-9. 2005
    ..Together, the promiscuous activity of FACL and PKSs provides new opportunities to expand the repertoire of natural products...
  75. ncbi Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: properties, function and occurrence of wax ester synthases/acyl-CoA: diacylglycerol acyltransferases
    Marc Wältermann
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, 48149 Munster, Germany
    Biochimie 89:230-42. 2007
    ..Furthermore, recent advances in understanding the physiological functions of WS/DGATs in their natural hosts including pathogenic Mycobacterium tuberculosis were discussed...
  76. ncbi Aminoacyl-coenzyme A synthesis catalyzed by adenylation domains
    Uwe Linne
    Philipps Universitat Marburg, Fachbereich Chemie Biochemie, Hans Meerwein Strasse, 35032 Marburg, Germany
    FEBS Lett 581:905-10. 2007
    ..Product quantification for kinetic determination was carried out by ESI-SIM-MS. Our results allow speculation as to evolutionary relationships within the large class of adenylate forming enzymes...
  77. ncbi An improved assay of 3-hydroxy-3-methylglutaryl-CoA reductase activity in Reuber H35 hepatoma cells without microsomes isolation
    M C García-Pelayo
    Department of Biochemistry and Molecular Biology, University of Granada, Spain
    Analyst 125:1583-5. 2000
    ..Our modified method was very sensitive and reproducible, because very low specific activity (about 15-100 pmol min-1 per mg protein) could be quantified in different assay conditions obtaining similar values...
  78. pmc Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling
    Madhulika Jain
    Department of Microbiology and Immunology, Program in Microbial Pathogenesis and Host Defense, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 104:5133-8. 2007
    ..Our results suggest that the shift to host lipid catabolism during infection allows for increased virulence lipid anabolism by the bacterium...
  79. ncbi Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia
    Claudia Maerker
    Institute of Microbiology, University of Hannover, Germany
    FEBS J 272:3615-30. 2005
    ..Therefore, an impact of methylcitrate synthase in the infection process is discussed...
  80. pmc Biochemical and structural characterization of an essential acyl coenzyme A carboxylase from Mycobacterium tuberculosis
    Gabriela Gago
    Microbiology Division, Instituto de Biologia Molecular y Celular de Rosario IBR, Universidad Nacional de Rosario, Suipacha 531, Rosario, Argentina
    J Bacteriol 188:477-86. 2006
    ..The addition of the epsilon subunit, which binds tightly to the alpha-beta subcomplex, is essential for gaining maximal enzyme activity...
  81. ncbi Blockade of arachidonic acid incorporation into phospholipids induces apoptosis in U937 promonocytic cells
    Rebeca Pérez
    Institute of Molecular Biology and Genetics, Spanish Research Council and University of Valladolid School of Medicine, 47003 Valladolid, Spain
    J Lipid Res 47:484-91. 2006
    ....
  82. ncbi A role for the malonyl-CoA/long-chain acyl-CoA pathway of lipid signaling in the regulation of insulin secretion in response to both fuel and nonfuel stimuli
    Raphael Roduit
    Molecular Nutrition Unit, Department of Nutrition, University of Montréal and the Centre Hospitalier de l Université de Montréal, Montreal, Quebec, Canada
    Diabetes 53:1007-19. 2004
    ..The data show the existence of important links between the metabolic coupling factor malonyl-CoA, the partitioning of fatty acids, and the stimulation of insulin secretion to both fuel and nonfuel stimuli...
  83. ncbi The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A
    Andrew M Gulick
    Hauptman Woodward Medical Research Institute, Buffalo, New York 14203 1149, USA
    Biochemistry 42:2866-73. 2003
    ..Additionally, we provide a structural explanation for the recently shown control of enzyme activity by acetylation of an active site lysine...
  84. pmc Thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase of Acinetobacter sp. strain ADP1
    Stefan Uthoff
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, D 48149 Munster, Germany
    Appl Environ Microbiol 71:790-6. 2005
    The bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT) from Acinetobacter sp...
  85. doi Splice variant-dependent regulation of beta-cell sodium-calcium exchange by acyl-coenzyme As
    Kevin S C Hamming
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Mol Endocrinol 22:2293-306. 2008
    ....
  86. pmc Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae
    Daniel Sorger
    Institut fur Biochemie, Technische Universitat Graz, A 8010 Graz, Austria
    J Bacteriol 184:519-24. 2002
    ..In conclusion, at least three different pathways are involved in the formation of storage TAG in the yeast...
  87. ncbi Acyl coenzyme A esters differentially activate cardiac and beta-cell adenosine triphosphate-sensitive potassium channels in a side-chain length-specific manner
    Jocelyn E Manning Fox
    Department of Pharmalology, University of Alberta, Edmonton, Alberta, Canada
    Metabolism 52:1313-9. 2003
    Recent evidence demonstrates that long-chain acyl coenzyme A esters (CoAs) activate cardiac and beta-cell plasma-membrane (pmK(ATP)) adenosine triphosphate (ATP)-sensitive potassium channels...
  88. pmc Novel pathway for catabolism of the organic sulfur compound 3,3'-dithiodipropionic acid via 3-mercaptopropionic acid and 3-Sulfinopropionic acid to propionyl-coenzyme A by the aerobic bacterium Tetrathiobacter mimigardefordensis strain DPN7
    Jan Hendrik Wübbeler
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, 48149 Munster, Germany
    Appl Environ Microbiol 74:4028-35. 2008
    ..Therefore, a new pathway is proposed for the catabolism of DTDP via 3MP, 3SP, and 3SP-CoA toward propionyl-CoA, which is then further catabolized via the 2-methylcitric acid cycle in T. mimigardefordensis...
  89. ncbi Conjugated and non-conjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: cholesterol acyltransferase activity
    Cheuk Kai Lam
    Food and Nutritional Sciences Programme of Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    Atherosclerosis 198:85-93. 2008
    ..Dietary CLA was associated with the least intestinal acyl coenzyme A: cholesterol acyltransferase (ACAT) activity followed by LN, LA and CLN in a decreasing trend...
  90. doi A 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus enhances acyl exchange between acyl-CoA and phosphatidylcholine
    Olga P Yurchenko
    Agricultural Lipid Biotechnology Program, Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada
    Plant Biotechnol J 7:602-10. 2009
    ..Over-expression of ACBP during seed development may represent a useful biotechnological approach for altering the FA composition of seed oil...
  91. pmc Defective peroxisomal catabolism of branched fatty acyl coenzyme A in mice lacking the sterol carrier protein-2/sterol carrier protein-x gene function
    U Seedorf
    Institute for Arteriosclerosis Research, Westfalian Wilhelms University, D 48129 M unster, Germany
    Genes Dev 12:1189-201. 1998
    ..These results corresponded to high-affinity binding of phytanoyl-CoA to the recombinant rat SCP2 protein, as well as high 3-ketopristanoyl-CoA thiolase activity of the recombinant rat SCPx protein...
  92. ncbi ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA
    Ka Chun Leung
    Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Plant Mol Biol 55:297-309. 2004
    ..Their predicted cytosol localization suggests that they could maintain an oleoyl-CoA pool in the cytosol or transport oleoyl-CoA from the plastids to the ER in plant lipid metabolism...
  93. ncbi Long chain CoA esters as competitive antagonists of phosphatidylinositol 4,5-bisphosphate activation in Kir channels
    Markus Rapedius
    Institute of Physiology II, Friedrich Schiller University Jena, Teichgraben 8, 07743 Jena, Germany
    J Biol Chem 280:30760-7. 2005
    ..g. LC-CoA production) could have profound and widespread effects on cellular electrical activity...
  94. pmc The critical role of Arabidopsis electron-transfer flavoprotein:ubiquinone oxidoreductase during dark-induced starvation
    Kimitsune Ishizaki
    Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
    Plant Cell 17:2587-600. 2005
    ....
  95. doi Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg im Breisgau, Germany
    J Biol Chem 283:32283-93. 2008
    ..In combination with molecular modeling, two signature sequences were identified that presumably contribute to the substrate specificity of these enzymes...
  96. ncbi Arabidopsis ACBP3 is an extracellularly targeted acyl-CoA-binding protein
    Ka Chun Leung
    Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Planta 223:871-81. 2006
    ....
  97. pmc Metabolic regulation of sodium-calcium exchange by intracellular acyl CoAs
    Michael J Riedel
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada
    EMBO J 25:4605-14. 2006
    ..Additionally, our findings have broad implications for the coupling of Ca(2+) homeostasis to fat metabolism in a variety of tissues...
  98. ncbi Kir6.2 polymorphisms sensitize beta-cell ATP-sensitive potassium channels to activation by acyl CoAs: a possible cellular mechanism for increased susceptibility to type 2 diabetes?
    Michael J Riedel
    Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 52:2630-5. 2003
    ....
  99. ncbi The crotonase superfamily: divergently related enzymes that catalyze different reactions involving acyl coenzyme a thioesters
    H M Holden
    Departments of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    Acc Chem Res 34:145-57. 2001
    ..This apparently is accomplished by two structurally conserved peptidic NH groups that provide hydrogen bonds to the carbonyl moieties of the acyl-CoA substrates and form an "oxyanion hole"...
  100. ncbi Single amino acid substitutions at the acyl-CoA-binding domain interrupt 14[C]palmitoyl-CoA binding of ACBP2, an Arabidopsis acyl-CoA-binding protein with ankyrin repeats
    M L Chye
    Department of Botany, University of Hong Kong, China
    Plant Mol Biol 44:711-21. 2000
    ..Analysis of the ACBP2 promoter revealed that, like the ACBP1 promoter, it lacks a TATA box suggesting the possibility of a housekeeping function for ACBP2 in plant lipid metabolism...
  101. ncbi Molecular and structural analysis of two novel mutations in a patient with mut(-) methylmalonyl-CoA deficiency
    J F Benoist
    Service de Biochimie Hormonologie, Assitanace Publique Hôpitaux de Paris, Hopital Robert Debre, 48 Bd Serurier, 75019 Paris, France
    Mol Genet Metab 72:181-4. 2001
    ..These two mutations and three previously published ones (H627N, A191E, Y231N) were mapped onto a three-dimensional homology model of the human MCM constructed from the crystal structure of the Propionibacterium shermanii enzyme...

Research Grants74

  1. Mechanisms of tubular atrophy in renal disease
    Jeffrey R Schelling; Fiscal Year: 2012
    ..The purpose of this project is to determine why kidneys fail. In particular, we plan to study biochemical pathways that prevent kidneys cells from dying, which may lead to new therapies to halt the progression of kidney diseases. ..
  2. Functional Analysis of ACAT
    Ta Yuan Chang; Fiscal Year: 2013
    ..As a key enzyme in cellular cholesterol metabolism, ACAT utilizes cholesterol and long-chain fatty acyl coenzyme A to produce cholesteryl esters, and is allosterically activated by its own substrate, cholesterol, to guard ..
  3. Elucidating Protein Folding Mechanisms
    Jay R Winkler; Fiscal Year: 2010
    ..Aim 2 involves joint experimental/computational studies of the folding mechanism of acyl coenzyme A binding protein...
  4. Beauveriolide-Derived Cyclodepsipeptides as a New Class of Anti-Alzheimer's Drugs
    Paul Wentworth; Fiscal Year: 2010
    ..This project describes the development of a new class of compounds that may prevent and/or AD. ..
  5. ACAT inhibitors regulate palmitoylated APP and Abeta production
    Dora M Kovacs; Fiscal Year: 2013
    ..Collectively, the goal of these experiments is to provide the necessary mechanistic and in vivo data for further development of ACAT inhibitors as a therapeutic strategy for AD and perhaps cardiovascular disease. ..
  6. SPHINGOLIPIDS AND STEROLS--METABOLISM AND INTERACTIONS
    Seiichi Matsuda; Fiscal Year: 2000
    ..studies of the actions of a variety of oxysterols on cholesterol esterification and their actions on acyl coenzyme A-cholesterol acyltransferase, studies of relevance to the intestinal absorption of cholesterol and the ..
  7. ALTERED MEMBRANE PHOSPHOLIPIDS AND CARDIAC FUNCTION
    DIANE REIBEL; Fiscal Year: 1990
    ..in ischemic hearts include the content of myocardial phospholipids, free fatty acids and long-chain fatty acyl coenzyme A and carnitine esters...
  8. Parenteral Medium Chain Triglycerides in the Premature
    Eberhard Schmidt Sommerfeld; Fiscal Year: 2002
    ..The long term goal is to determine whether the addition of MCT and L-carnitine to TPN will improve the nutrition of premature infants during a critical period of their lives. ..
  9. ENZYMES IN ANIMAL METABOLISM OF OXALATE DERIVATIVES
    Gordon Hamilton; Fiscal Year: 1990
    ..that will be investigated in the proposed research include: (1) a membrane bound amidase that cleaves S- acyl coenzyme A derivatives to S-acylcysteamines will be isolated and characterized, (2) the reactivity of various S-..
  10. CELL MUTANTS DEFECTIVE IN CHOLESTEROL ESTER FORAMTION
    Ta Yuan Chang; Fiscal Year: 2003
    b>Acyl coenzyme A: cholesterol acyltransferase (ACAT) catalyzes the formation of intracellular cholesterol esters...
  11. Liquid Chormatograph Mass Spectrometer (LC-MS)
    MURRAY JOHNSTON; Fiscal Year: 2002
    ..Riordan) model studies of acetyl coenzyme A synthase; (Thorpe) flavoproteins in fatty acid metabolism, acyl coenzyme A dehydrogenases and related enzymes of fatty acid beta-oxidation, and sulfhydryl oxidase catalysis...
  12. ENERGY METABOLISM AS A REGULATOR OF THE MYOCARDIUM
    KEITH COX; Fiscal Year: 2003
    ..The co-sponsors, Jeffery Molkentin and Jeffrey Robbins have actively and successfully led research programs using mouse models to study the development and progression of cardiac hypertrophy is disease. ..
  13. REGULATION OF MEMBRANE PHOSPHOLIPID STRUCTURE
    CHARLES ROCK; Fiscal Year: 1992
    ....
  14. Metabolic Engineering of Yeast for Polyketide Production
    JAMES KEALEY; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATION: A generic overproducing yeast strain will enable production of commercially valuable polyketides with significant cost and time savings over existing native host organisms and production methods. ..
  15. REGULATION OF HEPATIC CHOLESTEROL ESTER METABOLISM
    Sandra Erickson; Fiscal Year: 1990
    ..Major emphasis is on the regulation of cholesterol esterification by the enzyme acyl coenzyme A: cholesterol acyltransferase (ACAT)...
  16. Enzymes of Skin Lipid Metabolism
    David Russell; Fiscal Year: 2009
    ..by analogy to plants and lower organisms is thought to involve the sequential actions of two enzymes, a fatty acyl coenzyme A (CoA) reductase and a wax ester synthase...
  17. Attenuating sterol synthesis in WT and NPC mice brains
    Ta Yuan Chang; Fiscal Year: 2005
    ..Specific Aim 3: To examine the long-term effect of SSI CP-340868 at 2 different doses on life spans of normal and NPC1 mice. ..
  18. XALD: Role of Very Long Chain Fatty Acyl-CoA Synthetases
    Paul Watkins; Fiscal Year: 2006
    ..Furthermore, they will facilitate elucidation of the role of enzymes belonging to the VLACS or BG families in XALD. [unreadable] [unreadable]..
  19. Brain Uptake and Utilization of Fatty Acids and Lipids
    Paul Watkins; Fiscal Year: 2004
    ..Mol. Neurosci., which has committed to publish the proceedings of the proposed meeting. ..
  20. HIV Protease Inhibitors: Insulin Resistance & Insulysin
    FREDERICK HAMEL; Fiscal Year: 2007
    ..If insulin-degrading enzyme is involved, HIV protease inhibitors could be developed or screened for minimal reactivity with IDE. ..
  21. Inflammation and Infection as Risk Factors in Stroke
    Mitchell Elkind; Fiscal Year: 2006
    ..Nationally recognized experts in stroke epidemiology and basic vascular biology will serve as mentors for these studies. ..
  22. Investigation of ACAT Functions Using Knockout
    Robert Farese; Fiscal Year: 2006
    ..The fourth aim is to determine the function of ACAT enzymes in embryonic development by studying ACAT gene expression during development and investigating the cause of lethality of ACAT1-/-ACAT2-/- embryos. ..
  23. NEURONAL PLASTICITY AND THE CONTROL OF EYE POSITION
    NEAL BARMACK; Fiscal Year: 2005
    ..We will use "differential display" to identify mRNAs in dorsal cap neurons whose proteins might be responsible for two functions: 1) Axonal transport of CRF , and 2) Calcium binding. ..
  24. Glial Activation Following Perinatal Hypoxia-Ischemia
    Mark Wainwright; Fiscal Year: 2008
    ..The convergent pharmacological and genetic approaches to understanding the mechanisms of glial activation in acute brain injury may lead to the identification of new therapeutic targets in perinatal asphyxia. ..
  25. REGULATION OF FOLATE METABOLISM
    ROWENA MATTHEWS; Fiscal Year: 2008
    ..We predict that phosphorylation will play an important role in modulating the flux of one-carbon units. ..
  26. MECHANISMS OF ACTIONS OF ALCOHOL AND OMEGA 3-FATTY ACIDS
    Raj Lakshman; Fiscal Year: 2004
    ....
  27. BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDS
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
  28. LOCALIZATION OF THE GOLGI COMPLEX
    Suzanne Pfeffer; Fiscal Year: 2001
    ....
  29. Inflammatory and Infectious Burden and Risk of Stroke
    Mitchell Elkind; Fiscal Year: 2008
    ..The cross-sectional associations between inflammatory and infectious markers and quantitative MRI abnormalities will also be assessed. ..
  30. Gordon Research Sponsored Conference on Cobalamins
    Ruma Banerjee; Fiscal Year: 2003
    ....
  31. Functional Analysis of MGAT Enzymes
    Robert V Farese; Fiscal Year: 2010
    ..Additionally, if MGAT2 deficiency protects against diet-induced obesity, it may also protect against diet-induced insulin resistance and type 2 diabetes. ..
  32. ENZYMATIC REACTIONS OF PLASMA LIPOPROTEINS
    Henry J Pownall; Fiscal Year: 2010
    ....
  33. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    Stephen C Winans; Fiscal Year: 2010
    ..Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis. ..
  34. Levels of Inflammatory Markers in Treatment of Stroke
    Mitchell Elkind; Fiscal Year: 2008
    ..abstract_text> ..
  35. Identification of Genes that Regulate Cellular Lipid Storage
    Robert Farese; Fiscal Year: 2008
    ..The knowledge gained from these studies could have direct implications for treatment modalities for metabolic syndrome or type 2 diabetes. [unreadable] [unreadable] [unreadable]..
  36. Prevent HIV protease inhibitor-induced atherosclerosis by Berberine
    HUIPING ROSE ZHOU; Fiscal Year: 2010
    ..Completion of these specific aims will help identify and establish new therapeutic strategies for HIV infection. ..
  37. Alcohol abuse in a small rodent neuroAIDS model
    Johnny He; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  38. Effects of ethanol on AMP kinase signaling
    David Crabb; Fiscal Year: 2008
    ..It may also shed light on the pathogenesis of non-alcoholic fatty liver disease. [unreadable] [unreadable] [unreadable]..
  39. FATTY ACID BINDING PROTEINS-LIGAND SPECIFICITY
    Friedhelm Schroeder; Fiscal Year: 2010
    ..Differences in this nuclear receptor/fatty acid energy regulation could contribute to the pathogenesis of obesity, insulin resistance, type 2 diabetes mellitus, and hyperlipidemic conditions. ..
  40. Membrane Microdomains in Prostate Cancer
    Michael Freeman; Fiscal Year: 2009
    ..They are also designed to identify new signaling proteins of potential relevance to PCa, thereby widening possibilities for therapeutic intervention. ..
  41. Nutritional Treatment of Fat Oxidation Defect in Mice
    Henri Brunengraber; Fiscal Year: 2009
    ..To improve the survival of newborn MTP -/- mice. ..
  42. ASYMMETRIC DISTRIBUTION OF CHOLESTEROL IN MEMBRANES
    Friedhelm Schroeder; Fiscal Year: 2010
    ..If this process were understood, new therapeutic targets for increasing cholesterol efflux for lowering plasma cholesterol could potentially be developed. ..
  43. The Electrochemistry of Diheme Cytochrome c Peroxidases
    Sean J Elliott; Fiscal Year: 2010
    ..Further, our study of triheme CCPs will elucidate the CCP machinery which is unique to pathogens such as Salmonella enterica and Yersinia pestis, providing new insights into their biochemical pathways. ..
  44. Molecular Analysis of the CCC185 Golgin
    Suzanne R Pfeffer; Fiscal Year: 2010
    ..This work has broad implications for our understanding of vesicle docking and fusion events within the secretory and endocytic pathways that are essential for normal human health and disease. ..
  45. Metabolomic and Isotopomer Analysis of Xenobiotic Stress
    Henri Brunengraber; Fiscal Year: 2009
    ..This will be achieved by administering low doses of 2H20 and acetaminophen, followed by measuring the 2H-labeling of urinary acetaminophen-glutathione adduct. ..
  46. Light & Heavy Alcohol, Paraoxonase & Oxidized LDL Status
    Raj Lakshman; Fiscal Year: 2008
    ..These investigations are likely to lead to new insights in our understanding of the cardioprotective effects of ligh drinking and proatherogenic effects of heavy drinking both at clinical and at molecular levels. ..
  47. INTRACELLULAR TRANSPORT--THE MANNOSE-PHOSPHATE RECEPTOR
    Suzanne Pfeffer; Fiscal Year: 2008
    ..These studies have important implications for our understanding of growth control and antigen processing, and will provide fundamental information regarding the mechanism of receptor trafficking in mammalian cells. ..
  48. HIV Protease Inhibitors, the Unfolded Protein Response and Atherosclerosis
    Huiping Zhou; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  49. 2003 Kern Aspen Lipid Conference: Integrative Mechanisms
    Lawrence Rudel; Fiscal Year: 2003
    ..The timeliness of this meeting is viewed as a strength. ..
  50. REGULATION OF EXPRESSION OF ALDH2
    David Crabb; Fiscal Year: 2003
    ..These studies should further our understanding of the tissue-specific control of ALDH2 and the potential that dietary factors have on its expression. ..
  51. FATTY ACID AND PEPTIDE AMIDATION--A SHARED MECHANSIM
    Gregory Mueller; Fiscal Year: 2003
    ..Demonstrating that oleamide biosynthesis proceeds through PAM, or via an alternative pathway, will provide a basis for improving the diagnosis and treatment of sleep disorders. ..
  52. INVESTIGATIONS ON GLUCONEOGENESIS
    Henri Brunengraber; Fiscal Year: 2003
    ..We hope that the data of our investigations will help to devise new strategies for improving the treatment of type I diabetic patients. ..
  53. RECOVERY OF DAMAGE TO THE VESTIBULAR-AUDITORY SYSTEM
    NEAL BARMACK; Fiscal Year: 2003
    ..abstract_text> ..
  54. GC Continuous Flow Isotope Ratio Mass Spectrometer
    Henri Brunengraber; Fiscal Year: 2003
    ..The costs of operating and maintaining the instrument will be shared by the users. The availability of this instrument will considerably increase the scope of the basic science and clinical investigations conducted by the applicants. ..
  55. 14th International Congress on Flavins and Flavoproteins
    ROWENA MATTHEWS; Fiscal Year: 2002
    ..Tea will be served at each poster session. Presentations were selected by an International Organizing Committee with an eye to a diverse representation of speakers at the meeting. ..
  56. GENE-NUTRIENT INTERACTIONS IN REDOX HOMEOSTASIS
    Ruma Banerjee; Fiscal Year: 2002
    ....
  57. CAROTENOID BINDING PROTEIN AND CAROTENOID METABOLISM
    Raj Lakshman; Fiscal Year: 2002
    ..2. Molecular biology techniques to measure CCBP mRNA levels in different tissues and to clone its cDNA. 3. HPLC procedures for quantification of various carotenoids and retinoids. ..
  58. Gordon Research Conference on Tetrapyrroles
    Gloria Ferreira; Fiscal Year: 2002
    ..Strengths of this ongoing Gordon conference are its disciplinary diversity, focus on new unpublished work, time for discussion, informal atmosphere, and ban on dissemination of conference proceeding. ..
  59. CELL SIGNALING IN BLADDER HYPERTROPHY
    Michael Freeman; Fiscal Year: 2004
    ..New therapies based on the underlying mechanisms of obstructive uropathy will diminish the need for surgical intervention and improve the quality of life for patients with non-malignant bladder dysfunction. ..
  60. Structure-Function Analysis of Enzymes
    Hazel Holden; Fiscal Year: 2004
    ....
  61. EXPANDED NEWBORN SCREENING FOR METABOLIC DISORDERS
    Susan Waisbren; Fiscal Year: 2006
    ..Primary data from parent interviews and the New England Newborn Screening Program, as well as secondary data from published reports and a panel of experts will be used as inputs to the cost-effectiveness model. [unreadable] [unreadable]..
  62. Novel regulation of adipogenesis: agouti & melanocortins
    Randall Mynatt; Fiscal Year: 2007
    ..abstract_text> ..
  63. BIOSYNTHESIS OF SOME MICROBIAL METABOLITES
    Ronald Parry; Fiscal Year: 2007
    ..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
  64. Dynamic Metabolomics via Isotopomer Analysis
    Henri Brunengraber; Fiscal Year: 2006
    ..Second, we provide a rigorous test of the hypothesis that the mathematical analysis of mass isotopomer data enhances concentration-based metabolomics to provide new avenues for understanding metabolic diseases. ..
  65. Transcarboxylase: Strucuture, Flexibility and Mechanism
    Paul Carey; Fiscal Year: 2006
    ..3. To map inter-subunit and active site-active site distances in holo TC using fluorescence resonance energy transfer. ..
  66. Cell type gene delivery and alcoholic liver disease
    Michael Wheeler; Fiscal Year: 2006
    ..In addition, through didactic training, and interactions with his mentor and key faculty, the applicant will acquire new skills that will allow him to become a successful member of the alcohol research community. ..
  67. Heat shock proteins and alcohol induced liver injury
    Pranoti Mandrekar; Fiscal Year: 2006
    ....
  68. Biochemical and Metabolic Properties of 10-HETE
    ARTHUR SPECTOR; Fiscal Year: 2006
    ..The ultimate goal of this project is to provide the additional mechanistic insight necessary to develop new therapeutic approaches to overcome the pathological actions of 20-HETE. ..
  69. ETHANOL OXIDATION IN GENETICALLY MODIFIED CELLS
    David Crabb; Fiscal Year: 2002
    ..This study should answer the question of how the presence of different isozymes of ADH modulates alcohol metabolic rates and intracellular acetaldehyde. ..
  70. Convergence of Nuclear Receptor and Forkhead Signaling
    Paul Dowell; Fiscal Year: 2004
    ..The excellent research environment at Johns Hopkins coupled with an extensive collaborative team of experts in relevant fields of study is ideal for successful completion of the proposed work. ..