cobamides

Summary

Top Publications

  1. pmc The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation
    T A Bobik
    Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA
    J Bacteriol 181:5967-75. 1999
  2. pmc The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme
    Nicole R Buan
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    J Bacteriol 186:5708-14. 2004
  3. pmc Genetic analysis of the protein shell of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella
    Shouqiang Cheng
    Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA
    J Bacteriol 193:1385-92. 2011
  4. pmc Conversion of cobinamide into adenosylcobamide in bacteria and archaea
    Jorge C Escalante-Semerena
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 189:4555-60. 2007
  5. pmc Identification of a reactivating factor for adenosylcobalamin-dependent ethanolamine ammonia lyase
    Koichi Mori
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    J Bacteriol 186:6845-54. 2004
  6. pmc Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome
    Shaun R Brinsmade
    Department of Bacteriology, University of Wisconsin Madison, 53726 4087, USA
    J Bacteriol 187:8039-46. 2005
  7. pmc B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli
    A Jordan
    Department of Biochemistry I, Medical Nobel Institute, MBB, Karolinska Institute, S 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 94:13487-92. 1997
  8. ncbi Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg im Breisgau, Germany
    J Biol Chem 283:32283-93. 2008
  9. pmc Comparative and functional genomic analysis of prokaryotic nickel and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters
    Dmitry A Rodionov
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 188:317-27. 2006
  10. pmc The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins
    Michael J Gray
    Department of Bacteriology, University of Wisconsin, 6478 Microbial Sciences Building, 1550 Linden Drive, Madison, WI 53706, USA
    Mol Microbiol 70:824-36. 2008

Research Grants

Detail Information

Publications168 found, 100 shown here

  1. pmc The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation
    T A Bobik
    Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA
    J Bacteriol 181:5967-75. 1999
    ..The specific function of these unusual polyhedral bodies was not determined, but some possibilities are discussed...
  2. pmc The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme
    Nicole R Buan
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    J Bacteriol 186:5708-14. 2004
    ..We propose that the EutT protein is a unique metal-containing ATP:co(I)rrinoid adenosyltransferase. It is unclear whether the metal ion plays a structural or catalytic role...
  3. pmc Genetic analysis of the protein shell of the microcompartments involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella
    Shouqiang Cheng
    Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA
    J Bacteriol 193:1385-92. 2011
    ..Interestingly, a pduK deletion caused MCP aggregation, suggesting a role in the spatial organization of MCP within the cytoplasm or perhaps in segregation at cell division...
  4. pmc Conversion of cobinamide into adenosylcobamide in bacteria and archaea
    Jorge C Escalante-Semerena
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 189:4555-60. 2007
  5. pmc Identification of a reactivating factor for adenosylcobalamin-dependent ethanolamine ammonia lyase
    Koichi Mori
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    J Bacteriol 186:6845-54. 2004
    ..Thus, it was concluded that EutA is the only component of the reactivating factor for ethanolamine ammonia lyase and that reactivation and activation occur through the exchange of modified coenzyme for free intact adenosylcobalamin...
  6. pmc Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome
    Shaun R Brinsmade
    Department of Bacteriology, University of Wisconsin Madison, 53726 4087, USA
    J Bacteriol 187:8039-46. 2005
    ..We propose the metabolosome is needed to concentrate low levels of ethanolamine catabolic enzymes, to keep the level of toxic acetaldehyde low, to generate enough acetyl-CoA to support cell growth, and to maintain a pool of free CoA...
  7. pmc B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli
    A Jordan
    Department of Biochemistry I, Medical Nobel Institute, MBB, Karolinska Institute, S 17177 Stockholm, Sweden
    Proc Natl Acad Sci U S A 94:13487-92. 1997
    ..From the conservation of substrate- and effector-binding residues we propose that the B12-dependent class II enzymes contain a similar barrel...
  8. ncbi Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg im Breisgau, Germany
    J Biol Chem 283:32283-93. 2008
    ..In combination with molecular modeling, two signature sequences were identified that presumably contribute to the substrate specificity of these enzymes...
  9. pmc Comparative and functional genomic analysis of prokaryotic nickel and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters
    Dmitry A Rodionov
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 188:317-27. 2006
    ..Experimental analysis confirmed metal transport activity for three members of this family and demonstrated significant activity for a basic module (CbiMN) of the Salmonella enterica serovar Typhimurium transporter...
  10. pmc The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins
    Michael J Gray
    Department of Bacteriology, University of Wisconsin, 6478 Microbial Sciences Building, 1550 Linden Drive, Madison, WI 53706, USA
    Mol Microbiol 70:824-36. 2008
    ..Phylogenetic data led us to conclude that CbiZ is an enzyme of archaeal origin that was horizontally transferred to bacteria. Reasons why some bacteria may contain both types of Cbi salvaging systems are discussed...
  11. ncbi Comparative genomics of the vitamin B12 metabolism and regulation in prokaryotes
    Dmitry A Rodionov
    State Scientific Center GosNIIGenetika, Moscow 113545, Russia
    J Biol Chem 278:41148-59. 2003
    ..Moreover, B12 regulons of various bacteria are thought to include enzymes from known B12-dependent or alternative pathways...
  12. pmc Decyanation of vitamin B12 by a trafficking chaperone
    Jihoe Kim
    Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 0606, USA
    Proc Natl Acad Sci U S A 105:14551-4. 2008
    ....
  13. pmc A rationale for autoinduction of a transcriptional activator: ethanolamine ammonia-lyase (EutBC) and the operon activator (EutR) compete for adenosyl-cobalamin in Salmonella typhimurium
    D E Sheppard
    School of Life and Health Sciences, University of Delaware, Newark 19716
    J Bacteriol 176:1287-96. 1994
    ..Second, the maximum level of operon induction by excess CN-B12 is dictated by the level of EutR regulatory protein, regardless of the level of lyase...
  14. ncbi Accurate redetermination of the X-ray structure and electronic bonding in adenosylcobalamin
    Lizhi Ouyang
    Department of Physics, University of Missouri Kansas City, Kansas City, Missouri 64110, USA
    Inorg Chem 43:1235-41. 2004
    ..e., simplified cobalamins with the side chains a-f replaced by H atoms. Despite a qualitative agreement, a discrepancy in the calculated HOMO-LUMO gap is found...
  15. ncbi The biosynthesis of adenosylcobalamin (vitamin B12)
    Martin J Warren
    School of Biological Sciences, Queen Mary, University of London, Mile End Road, London, UK E1 4NS
    Nat Prod Rep 19:390-412. 2002
    ..This review covers some of the most important findings that have been made and provides the reader with a complete description of the transformation of uroporphyrinogen III into adenosylcobalamin (AdoCbl). 183 references are cited...
  16. ncbi Solution structure, enzymatic, and non-enzymatic reactivity of 3-isoadenosylcobalamin, a structural isomer of coenzyme B12 with surprising coenzymic activity
    Kenneth L Brown
    Department of Chemistry and Biochemistry, Clippinger Laboratories, Ohio University, Athens, OH 45701, USA
    J Inorg Biochem 98:287-300. 2004
    ....
  17. ncbi Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis
    Andrew D Lawrence
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    J Biol Chem 283:10813-21. 2008
    ..Kinetic and EPR analysis reveals that the enzyme proceeds via a semiquinone form. It is proposed that CobR may interact with the adenosyltransferase to overcome the large thermodynamic barrier required for co(II)rrin reduction...
  18. ncbi Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutase
    Monica Vlasie
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 277:18523-7. 2002
    ....
  19. ncbi [Radical-catalyzed enzymatic reactions: structure and mechanism of B12 enzymes]
    Tetsuo Toraya
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530
    Seikagaku 74:87-102. 2002
  20. ncbi Computational exploration of rearrangements related to the vitamin B12-dependent ethanolamine ammonia lyase catalyzed transformation
    Marija Semialjac
    Institut fur Chemie der Technischen Universitat Berlin, D 10623 Berlin, Germany
    J Am Chem Soc 124:8974-83. 2002
    ....
  21. ncbi Enzymatic radical catalysis: coenzyme B12-dependent diol dehydratase
    Tetsuo Toraya
    Department of Bioscience and Biotechnology, Faculty of Engineering Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    Chem Rec 2:352-66. 2002
    ..Based on the results, the fine mechanism of the enzyme and the energetic feasibility of enzymatic radical catalysis are described here...
  22. ncbi Time-resolved spectroscopic studies of B12 coenzymes: comparison of the influence of solvent on the primary photolysis mechanism and geminate recombination of methyl-, ethyl-, n-propyl-, and 5'-deoxyadenosylcobalamin
    Roseanne J Sension
    Departments of Chemistry and Physics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 109:21954-62. 2005
    ..In contrast, the magnitude of cage escape for the Me radical is much larger than anticipated on the basis of hydrodynamic arguments...
  23. ncbi Radical catalysis in coenzyme B12-dependent isomerization (eliminating) reactions
    Tetsuo Toraya
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    Chem Rev 103:2095-127. 2003
  24. ncbi Radical carbon skeleton rearrangements: catalysis by coenzyme B12-dependent mutases
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Chem Rev 103:2083-94. 2003
  25. ncbi Spectroscopic and computational studies of Co3+-corrinoids: spectral and electronic properties of the B12 cofactors and biologically relevant precursors
    Troy A Stich
    Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Am Chem Soc 125:5897-914. 2003
    ..Implications of these results with respect to enzymatic Co[bond]C activation are discussed...
  26. ncbi Mitochondrial vitamin B12-binding proteins in patients with inborn errors of cobalamin metabolism
    E Moras
    Department of Human Genetics, McGill University, Montreal, Que, Canada H3G 1A4
    Mol Genet Metab 90:140-7. 2007
    ..Furthermore, unique cobalamin binding profiles emerged demonstrating how known mutations in these patients affect cobalamin binding to as yet unidentified proteins...
  27. ncbi Synthesis of enantiomerically-pure [13C]aristeromycylcobalamin and its reactivity in dioldehydratase, glyceroldehydratase, ethanolamine ammonia-lyase and methylmalonyl-CoA mutase reactions
    Ulrich Weigl
    Institut fur Organische Chemie, Lehrstuhl für Biochemie Universität Karlsruhe Kaiserstrasse 12, 76 128 Karlsruhe, Germany
    Chemistry 9:652-60. 2003
    ..However, we found no evidence for the existence of a 6'-deoxyaristeromycyl radical during the reaction with [6'-(13)C]aristeromycylcobalamin. We conclude that the lifetime of this radical is still too short to be observed...
  28. pmc Alternative pathways for radical dissipation in an active site mutant of B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 45:2951-9. 2006
    ..These studies serve to emphasize the fine control exerted by Y243 in the vicinity of the substrate to minimize radical extinction in side reactions...
  29. ncbi Long-term outcome in methylmalonic acidurias is influenced by the underlying defect (mut0, mut-, cblA, cblB)
    Friederike Hörster
    Department of General Pediatrics, Division of Inborn Metabolic Diseases, University Children s Hospital, D 69120 Heidelberg, Germany
    Pediatr Res 62:225-30. 2007
    ..In addition, long-term outcome was dependent on the age cohort and cobalamin responsiveness...
  30. ncbi Coenzyme B(12) dependent glutamate mutase
    Karl Gruber
    Institut fur Chemie, Universitat Graz, Heinrichstrasse 28, Austria
    Curr Opin Chem Biol 6:598-603. 2002
    ..Crucial residues in the active site have been identified. Solution structure studies indicate the mechanism of B(12) binding to the apoenzyme...
  31. ncbi Stabilisation of methylene radicals by cob(II)alamin in coenzyme B12 dependent mutases
    Wolfgang Buckel
    Fachbereich Biologie, Philipps Universitat, 35032 Marburg, Germany
    Chemistry 12:352-62. 2005
    ..It is suggested that cob(II)alamin acts as a conductor by stabilising both the 5'-deoxyadenosyl radical and the product-related methylene radicals...
  32. ncbi Computational mutation analysis of hydrogen abstraction and radical rearrangement steps in the catalysis of coenzyme B12-dependent diol dehydratase
    Takashi Kamachi
    Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 819 0395, Japan
    Chemistry 13:7864-73. 2007
    ....
  33. pmc Delivery of tailor-made cobalamin to methylmalonyl-CoA mutase
    Vahe Bandarian
    Nat Chem Biol 4:158-9. 2008
  34. ncbi Interactions of diol dehydrase and 3',4'-anhydroadenosylcobalamin: suicide inactivation by electron transfer
    Olafur Th Magnusson
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    Biochemistry 41:1695-702. 2002
    ..This study provides an example of suicide inactivation of a radical enzyme brought about by a side reaction of an analogue of the radical intermediate...
  35. ncbi Critical role of arginine 160 of the EutB protein subunit for active site structure and radical catalysis in coenzyme B12-dependent ethanolamine ammonia-lyase
    Li Sun
    Department of Physics, Emory University, Atlanta, Georgia 30322, USA
    Biochemistry 47:5523-35. 2008
    ..The results indicate that the positive charge at the residue 160 side chain terminus is required for proper folding of EutB, assembly of a stable EAL oligomer, and catalysis in the assembled oligomer...
  36. ncbi A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase
    N Shibata
    Department of Life Science, Himeji Institute of Technology, Hyogo, Japan
    Structure 7:997-1008. 1999
    ..The crystal structure analysis of a diol dehydratase-cyanocobalamin complex was carried out in order to help understand the mechanism of action of this enzyme...
  37. ncbi When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutase
    Monica D Vlasie
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:8410-7. 2004
    ..These studies provide evidence for the critical role of active site residues in controlling radical reactivity and thereby suppressing inactivating side reactions...
  38. ncbi Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase
    Masaki Fukuoka
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama, Japan
    FEBS J 272:4787-96. 2005
    ..In contrast, neither adenosylethylcobalamin nor adeninylpentylcobalamin served as active coenzyme for either enzyme and did not undergo Co-C bond cleavage upon binding to apoenzymes...
  39. ncbi Role of protonation and of axial ligands in the reductive dechlorination of alkyl chlorides by vitamin B12 complexes. Reductive cleavage of chloroacetonitrile by Co(I) cobalamins and cobinamides
    Juan E Argüello
    Laboratoire d Electrochimie Moleculaire, Unite Mixte de Recherche Universite, CNRS No 7591, Université de Paris 7 Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France
    J Am Chem Soc 127:5049-55. 2005
    ..This amounts to a self-moderation effect: the more efficient catalysis, the slower its second step, i.e., the conversion of the alkylcobalt(III) complex into the alkylcobalt(II)...
  40. ncbi Catalytic roles of active-site amino acid residues of coenzyme B12-dependent diol dehydratase: protonation state of histidine and pull effect of glutamate
    Takashi Kamachi
    Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 812 8581, Japan
    J Am Chem Soc 126:16207-16. 2004
    ..Thus, it is predicted that His143 is not protonated, and therefore the main active-site amino acid residue that lowers the energy of the transition state for the OH group migration is determined to be Glu170...
  41. ncbi The cblD defect causes either isolated or combined deficiency of methylcobalamin and adenosylcobalamin synthesis
    Terttu Suormala
    Metabolic Unit, University Children s Hospital, Romergasse 8, CH 4058 Basel, Switzerland
    J Biol Chem 279:42742-9. 2004
    ..These findings indicate heterogeneity within the previously defined cblD mutant class and point to further complexity of intracellular cobalamin metabolism...
  42. ncbi Structure-based perspectives on B12-dependent enzymes
    M L Ludwig
    Biophysics Research Division, University of Michigan, Ann Arbor 48109 1055, USA
    Annu Rev Biochem 66:269-313. 1997
    ..of mercaptoethanesulfonate (coenzyme M) during methanogenesis have also been shown to contain histidine-ligated cobamides. In corrinoid iron-sulfur methyltransferases from acetogenic and methanogenic organisms, benzimidazole is ..
  43. ncbi Characterization of the product radical structure in the Co(II)-product radical pair state of coenzyme B12-dependent ethanolamine deaminase by using three-pulse 2H ESEEM spectroscopy
    Kurt Warncke
    Department of Physics, N201 Mathematics and Science Center, 400 Dowman Drive, Emory University, Atlanta, Georgia 30322, USA
    Biochemistry 44:3184-93. 2005
    ....
  44. ncbi Isotope effects for deuterium transfer between substrate and coenzyme in adenosylcobalamin-dependent glutamate mutase
    Mou Chi Cheng
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 44:2686-91. 2005
    ..The additional data provided by these experiments cause us to revise our interpretation of earlier UV-visible stopped-flow kinetic measurements of AdoCbl homolysis obtained with deuterated substrates...
  45. ncbi Acid-, base-, and lewis-acid-catalyzed heterolysis of methoxide from an alpha-hydroxy-beta-methoxy radical: models for reactions catalyzed by coenzyme B12-dependent diol dehydratase
    Libin Xu
    Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607, USA
    J Org Chem 70:9296-303. 2005
    ....
  46. ncbi Electronic structure studies of the adenosylcobalamin cofactor in glutamate mutase
    Amanda J Brooks
    Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Biochemistry 44:15167-81. 2005
    ....
  47. ncbi The synthesis and characterization of 8-methoxy-5'-deoxyadenosylcobalamin: a coenzyme B(12) analog which, following Co-C bond homolysis, avoids cyclization of the 8-methoxy-5'-deoxyadenosyl radical
    Kenneth M Doll
    Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA
    J Inorg Biochem 91:388-97. 2002
    ....
  48. pmc Identification of the substrate radical intermediate derived from ethanolamine during catalysis by ethanolamine ammonia-lyase
    Gunes Bender
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726, USA
    Biochemistry 47:11360-6. 2008
    ....
  49. ncbi Interconversion of (S)-glutamate and (2S,3S)-3-methylaspartate: a distinctive B(12)-dependent carbon-skeleton rearrangement
    S D Wetmore
    Contribution from the Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    J Am Chem Soc 123:7963-72. 2001
    ..Our calculations suggest that the enzyme may facilitate the rearrangement of (S)-glutamate through (partial) proton-transfer processes that control the protonation state of substituents in the migrating group...
  50. ncbi The B(12)-binding subunit of glutamate mutase from Clostridium tetanomorphum traps the nucleotide moiety of coenzyme B(12)
    M Tollinger
    Institute of Organic Chemistry, University of Innsbruck, Innsbruck, A-6020, Austria
    J Mol Biol 309:777-91. 2001
    ....
  51. ncbi Radical mechanisms of enzymatic catalysis
    P A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53705, USA
    Annu Rev Biochem 70:121-48. 2001
    ..Three examples are described of suicide inactivation through the formation of exceptionally stable free radicals at enzymatic active sites...
  52. ncbi Electron transfer in the substrate-dependent suicide inactivation of lysine 5,6-aminomutase
    K H Tang
    Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA
    Biochemistry 40:5190-9. 2001
    ..The alternative substrate beta-lysine induces inactivation at least 25 times faster than DL-lysine. The inactivation mechanism is compatible with the radical isomerization mechanism proposed to explain the action of 5,6-LAM...
  53. ncbi The coenzyme b12 analog 5'-deoxyadenosylcobinamide-gdp supports catalysis by methylmalonyl-coa mutase in the absence of trans-ligand coordination
    S Chowdhury
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
    J Biol Chem 276:1015-9. 2001
    ..These surprising results are in marked contrast to the effects ascribed to the corresponding lower axial histidine ligands in the cobalamin-dependent enzymes glutamate mutase and methionine synthase...
  54. pmc Characterization of a succinyl-CoA radical-cob(II)alamin spin triplet intermediate in the reaction catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 44:3153-8. 2005
    ..The interspin distance and the Euler angles were used to position this product radical into the active site of the enzyme...
  55. ncbi Role of Arg100 in the active site of adenosylcobalamin-dependent glutamate mutase
    Li Xia
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 43:3238-45. 2004
    ....
  56. ncbi Mirror "base-off" conformation of coenzyme B12 in human adenosyltransferase and its downstream target, methylmalonyl-CoA mutase
    Mamoru Yamanishi
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Am Chem Soc 127:526-7. 2005
    ..However, the coordination environment for cobalt in the two proteins is distinct, which is reflected in an approximately 40-fold difference in their affinity for the cofactor...
  57. ncbi A gene, cobA + hemD, from Selenomonas ruminantium encodes a bifunctional enzyme involved in the synthesis of vitamin B12
    P J Anderson
    Molecular and Cellular Biology, School of Biological Sciences, University of New England, Armidale, NSW 2351, Australia
    Gene 281:63-70. 2001
    ..The type found in S. ruminantium (cobA + hemD) is probably restricted to obligately anaerobic fermentative bacteria...
  58. ncbi Public health significance of elevated homocysteine
    Jacob Selhub
    Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
    Food Nutr Bull 29:S116-25. 2008
    ..Whether this denominator is homocysteine itself or homocysteine is merely a marker, remains to be determined...
  59. pmc Comparison of recombinant human haptocorrin expressed in human embryonic kidney cells and native haptocorrin
    Evelyne Furger
    Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, Villigen, Switzerland
    PLoS ONE 7:e37421. 2012
    ..The recombinant protein has properties very similar to native HC and although showing slightly different ligand binding kinetics, rhHC is valuable for further biochemical and structural studies...
  60. ncbi Radical use of Rossmann and TIM barrel architectures for controlling coenzyme B12 chemistry
    Daniel P Dowling
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biophys 41:403-27. 2012
    ..Using specific enzyme examples, we consider how nature employs the common TIM barrel fold and its Rossmann domain partner for radical-based chemistry...
  61. pmc Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle
    Yejun Han
    Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA
    Appl Environ Microbiol 78:6194-202. 2012
    ..Based on the highly conserved occurrence of single copies of MCE and MCM in Sulfolobaceae genomes, the M. sedula enzymes are likely to be representatives of these enzymes in the 3-HP/4-HB cycle in crenarchaeal thermoacidophiles...
  62. ncbi Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitch
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA
    Nat Struct Mol Biol 19:1182-4. 2012
    ....
  63. pmc Analysis of the Cob(II)alamin-5'-deoxy-3',4'-anhydroadenosyl radical triplet spin system in the active site of diol dehydrase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 45:14362-70. 2006
    ..Homolytic cleavage of the cobalt-carbon bond of the analogue in the absence of the substrate indicates that, in diol dehydrase, binding of the coenzyme to the protein weakens the bond prior to binding of the substrate...
  64. pmc A riboswitch regulates expression of the coenzyme B12-independent methionine synthase in Mycobacterium tuberculosis: implications for differential methionine synthase function in strains H37Rv and CDC1551
    Digby F Warner
    Molecular Mycobacteriology Research Unit, National Health Laboratory Service, P O Box 1038, Johannesburg 2000, South Africa
    J Bacteriol 189:3655-9. 2007
    ..Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis...
  65. pmc Growth inhibition of Sporomusa ovata by incorporation of benzimidazole bases into cobamides
    Kenny C Mok
    Department of Plant and Microbial Biology, University of California, Berkeley, California, USA
    J Bacteriol 195:1902-11. 2013
    Phenolyl cobamides are unique members of a class of cobalt-containing cofactors that includes vitamin B12 (cobalamin). Cobamide cofactors facilitate diverse reactions in prokaryotes and eukaryotes...
  66. pmc A disposable blood cyanide sensor
    Yong Tian
    Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019 0065, USA
    Anal Chim Acta 768:129-35. 2013
    ..5 to 200 μM. The method was validated against a microdiffusion approach and applied to the measurement of cyanide in rabbit and human blood. The disposable device permits field measurement of blood cyanide in <4 min...
  67. pmc Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity
    Jun Yan
    Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA
    Philos Trans R Soc Lond B Biol Sci 368:20120320. 2013
    ..g l(-1) extracellular cobamide (factor III), whereas the Sporomusa cultures produced phenolyl- and p-cresolyl-cobamides. Neither factor III nor the phenolic cobamides supported Dehalococcoides reductive dechlorination activity ..
  68. ncbi Ultrafast infrared spectral fingerprints of vitamin B12 and related cobalamins
    Alex R Jones
    Manchester Interdisciplinary Biocentre and Faculty of Life Sciences, University of Manchester, UK
    J Phys Chem A 116:5586-94. 2012
    ..Such coupling of motions throughout the ring makes specific peak assignments neither trivial nor always meaningful, suggesting our data should be regarded as IR spectral fingerprints...
  69. pmc Novel coenzyme B12-dependent interconversion of isovaleryl-CoA and pivalyl-CoA
    Valentin Cracan
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0600, USA
    J Biol Chem 287:3723-32. 2012
    ....
  70. ncbi Theoretical analysis of the diradical nature of adenosylcobalamin cofactor-tyrosine complex in B12-dependent mutases: inspiring PCET-driven enzymatic catalysis
    Pawel M Kozlowski
    Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA
    J Phys Chem B 114:5928-39. 2010
    ..e., [AdoCbl](*-)), rather than its neutral analogue, thus providing an efficient mode of cleavage that can help in understanding the origin of the catalytic effect in such enzymes...
  71. ncbi Magnetic field effect studies indicate reduced geminate recombination of the radical pair in substrate-bound adenosylcobalamin-dependent ethanolamine ammonia lyase
    Alex R Jones
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
    J Am Chem Soc 129:15718-27. 2007
    ..These findings have strong implications for the mechanism of RP stabilization and the unprecedented catalytic power of this important class of cobalamin-dependent enzymes...
  72. ncbi Release of a damaged cofactor from a coenzyme B12-dependent enzyme: X-ray structures of diol dehydratase-reactivating factor
    Naoki Shibata
    Graduate School of Life Science, University of Hyogo, 3 2 1 Kouto, Ako gun, Hyogo 678 1297, Japan
    Structure 13:1745-54. 2005
    ..The binding of the DD beta subunit to the DDR alpha subunit induces steric repulsion between the DDR alpha and DD alpha subunits that would lead to the release of a damaged cofactor from inactivated holoDD...
  73. ncbi Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay
    Pilar Ruiz-Sánchez
    Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    J Biol Inorg Chem 13:335-47. 2008
    ..Despite bearing a rather large metal complex on the beta-axial position, the cobamides in 5 and 7 are recognized by the corrinoid adenosyltransferase enzyme that catalyzes the formation of the ..
  74. doi A crystalline B12 dimer from beta-cyano-neocobyrate
    Shahzad Murtaza
    Institute of Organic Chemistry and Centre of Molecular Biosciences, University of Innsbruck, Innsbruck, Austria
    Chemistry 14:7521-4. 2008
  75. pmc Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase
    Kuo Hsiang Tang
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Biochemistry 48:8151-60. 2009
    ..The observation of the transient radical supports the hypothetical isomerization mechanism...
  76. pmc A human vitamin B12 trafficking protein uses glutathione transferase activity for processing alkylcobalamins
    Jihoe Kim
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 5066, USA
    J Biol Chem 284:33418-24. 2009
    ..This glutathione transferase activity of MMACHC is reminiscent of the methyltransferase chemistry catalyzed by the vitamin B(12)-dependent methionine synthase and is impaired in the cblC group of inborn errors of cobalamin disorders...
  77. pmc Functional analysis of the nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) enzyme, involved in the late steps of coenzyme B12 biosynthesis in Salmonella enterica
    Kathy R Claas
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Bacteriol 192:145-54. 2010
    ..the lower ligand base during the assembly of the nucleotide loop of adenosylcobalamin (AdoCbl) and other cobamides. Previously, mutational analysis identified a class of alleles (class M) that failed to restore AdoCbl ..
  78. pmc Adenosyl radical: reagent and catalyst in enzyme reactions
    E Neil G Marsh
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Chembiochem 11:604-21. 2010
    ....
  79. ncbi Picolylamine-methylphosphonic acid esters as tridentate ligands for the labeling of alcohols with the fac-[M(CO)3]+ core (M = 99mTc, Re): synthesis and biodistribution of model compounds and of a 99mTc-labeled cobinamide
    Stefan Mundwiler
    Institute of Inorganic Chemistry, University of Zurich, Switzerland
    Nucl Med Biol 32:473-84. 2005
    ..Biodistribution studies in mice with B(16) melanoma exhibited fast clearance with no specific tissue binding...
  80. ncbi Alpha-5,6-dimethylbenzimidazole adenine dinucleotide (alpha-DAD), a putative new intermediate of coenzyme B12 biosynthesis in Salmonella typhimurium
    Lori A Maggio-Hall
    Department of Bacteriology, University of Wisconsin, 1710 University Avenue, Madison, WI 53726 4087, USA
    Microbiology 149:983-90. 2003
    ..Evidence shows that extracts of S. typhimurium contain an as-yet unidentified dinucleotide pyrophosphatase that can cleave alpha-DAD into alpha-ribazole-5'-P and AMP; alpha-ribazole-5'-P can then enter the AdoCbl biosynthetic pathway...
  81. ncbi Purification and characterization of methylmalonyl-CoA mutase from a photosynthetic coccolithophorid alga, Pleurochrysis carterae
    Emi Miyamoto
    Department of Health Science, Kochi Women s University, 5 15 Eikokuji cho, Kochi 780 8515, Japan
    Comp Biochem Physiol B Biochem Mol Biol 138:163-7. 2004
    ..carterae enzyme occurs as a homodimer. Some properties of methylmalonyl-CoA mutase from P. carterae were studied...
  82. ncbi Structure and dynamics of the B12-binding subunit of glutamate mutase from Clostridium cochlearium
    B Hoffmann
    Institut of Organic Chemistry, University of Innsbruck, Innsbruck, Austria
    Eur J Biochem 263:178-88. 1999
    ....
  83. ncbi Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase
    Naoki Shibata
    Department of Life Science, Graduate School of Science, Himeji Institute of Technology, 3 2 1 Kouto, Kamigori, Ako gun, Hyogo 678 1297, Japan
    Biochemistry 41:12607-17. 2002
    ..Kinetic coupling of Co-C bond homolysis with hydrogen abstraction from the substrate shifts the equilibrium to dissociation...
  84. ncbi Reactions of nitric oxide with vitamin B12 and its precursor, cobinamide
    Vijay S Sharma
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0652, USA
    Biochemistry 42:8900-8. 2003
    ..The affinity of Cbi(II) for NO is so high that it is a very efficient NO trap and, consequently, may have important biomedical uses...
  85. ncbi S-Adenosylmethionine: a wolf in sheep's clothing, or a rich man's adenosylcobalamin?
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53726, USA
    Chem Rev 103:2129-48. 2003
  86. pmc Entropic origin of cobalt-carbon bond cleavage catalysis in adenosylcobalamin-dependent ethanolamine ammonia-lyase
    Miao Wang
    Department of Physics, Emory University, Atlanta, Georgia 30322, United States
    J Am Chem Soc 135:15077-84. 2013
    ..The catalysis is proposed to arise from an increase in protein configurational entropy along the reaction coordinate. ..
  87. ncbi Synthesis of adenosylcobinamide 2-chlorophenyl phosphate, a zwitterionic cobinamide phosphate analog of adenosylcobalamin en route to crystallizable cobinamides
    Wesley T White
    Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA
    J Inorg Biochem 91:371-87. 2002
    ..These compounds were characterized by HPLC, (1)H and (31)P NMR, UV-visible spectroscopy, and liquid secondary ionization mass spectroscopy...
  88. ncbi The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol
    Mamoru Yamanishi
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama, Japan
    Eur J Biochem 269:4484-94. 2002
    ..This is in clear contrast to diol dehydratase and accounts for the difference between the two enzymes in the susceptibility of suicide inactivation by glycerol...
  89. ncbi Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes
    R Daniel
    Institut für Mikrobiologie und Genetik der Georg August Universität, Gottingen, Germany
    FEMS Microbiol Rev 22:553-66. 1998
    ..Downstream of the dehydratase genes an open reading frame encoding a polypeptide of approximately 600 amino acids was identified which is apparently involved in the reactivation of suicide-inactivated enzyme...
  90. ncbi Radical catalysis of B12 enzymes: structure, mechanism, inactivation, and reactivation of diol and glycerol dehydratases
    T Toraya
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
    Cell Mol Life Sci 57:106-27. 2000
    ..These factors are a new type of molecular chaperone which participate in reactivation of the inactivated holoenzymes by mediating ATP-dependent exchange of the modified coenzyme for free intact coenzyme...
  91. ncbi Magnetic field effects on coenzyme B12-dependent enzymes: validation of ethanolamine ammonia lyase results and extension to human methylmalonyl CoA mutase
    S Taoka
    Biochemistry Department, University of Nebraska, Lincoln 68588 0664, USA
    Bioelectromagnetics 18:506-13. 1997
    ..Our studies suggest that putative magnetic field effects on physiological processes are not likely to be mediated by methylmalonyl-CoA mutase...
  92. ncbi Abiotic transformation of toxaphene by superreduced vitamin B12 and dicyanocobinamide
    Steffen Ruppe
    Department of Food Chemistry, Friedrich Schiller University of Jena, Dornburger Strasse 25, Jena, Germany
    Environ Sci Technol 38:3063-7. 2004
    ....
  93. ncbi [Characteristics of a clinical course of immune cytopenias with a high titer of autoantibodies to the microsomal antigen of the thyroid gland]
    Iu E Vinogradova
    Ter Arkh 76:81-5. 2004
    ..To ascertain the role of high antibodies (Ab) titers to microsomal antigen (MA) of the thyroid in clinical manifestations of cytopenic syndromes...
  94. ncbi Interaction of the substrate radical and the 5'-deoxyadenosine-5'-methyl group in vitamin B(12) coenzyme-dependent ethanolamine deaminase
    K Warncke
    Department of Physics, Emory University, 1001 Rollins Research Center, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Am Chem Soc 123:8564-72. 2001
    ....
  95. ncbi Adenosylcobalamin-dependent isomerases: new insights into structure and mechanism
    E N Marsh
    Department of Chemistry and Division of Biophysics, University of Michigan, Ann Arbor, MI 48109, USA
    Curr Opin Chem Biol 5:499-505. 2001
    ..Complementary data from kinetic, spectroscopic and theoretical studies have produced insights into the mechanism by which substrate radicals are generated at the active site, and the pathways by which they rearrange...
  96. ncbi Methylenetetrahydrofolate reductase C677T and A1298C polymorphisms and variations of homocysteine concentrations in patients with Behcet's disease
    M Messedi
    Unit of Research Molecular Bases of Human Diseases, Sfax Medicine College, Sfax 3029, Tunisia
    Gene 527:306-10. 2013
    ....
  97. ncbi DFT studies of trans and cis influences in the homolysis of the Co-C bond in models of the alkylcobalamins
    Penny P Govender
    Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, P O WITS, Johannesburg, 2050 South Africa
    J Phys Chem A 117:3057-68. 2013
    ..Corrin has the longest and weakest Co-C bond; this, together with a significant contraction of the Co-Nα after homolysis, is likely to be the origin of its relatively low BDE...
  98. ncbi Improvement of adenosylcobalamin production by metabolic control strategy in Propionibacterium freudenreichii
    Peng Wang
    National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, People s Republic of China
    Appl Biochem Biotechnol 167:62-72. 2012
    ..Overall, the findings show that the method is efficient in evaluating the fermentation of ado-cbl by propionibacteria...
  99. ncbi Initial step of B12-dependent enzymatic catalysis: energetic implications regarding involvement of the one-electron-reduced form of adenosylcobalamin cofactor
    Pawel M Kozlowski
    Department of Chemistry, University of Louisville, Louisville, KY 40292, USA
    J Biol Inorg Chem 17:293-300. 2012
    ....
  100. pmc Cobinamide chemistries for photometric cyanide determination. A merging zone liquid core waveguide cyanide analyzer using cyanoaquacobinamide
    Jian Ma
    Department of Chemistry and Biochemistry, University of Texas, 700 Planetarium Place, Arlington, TX 76019 0065, USA
    Anal Chim Acta 736:78-84. 2012
    ..The setup is readily used to measure thiocyanate with different reagents. We demonstrate applicability to real samples by analyzing human saliva samples and hydrolyzed extracts of apple seeds, peach pits, and almonds...
  101. pmc Thermolability of mutant MMACHC protein in the vitamin B12-responsive cblC disorder
    D S Froese
    Department of Biochemistry and Molecular Biology, University of Calgary, Alberta Children s Hospital Research Institute for Child and Maternal Health, Calgary, Alta, Canada
    Mol Genet Metab 100:29-36. 2010
    ....

Research Grants19

  1. MECHANISM OF ENZYME MEDIATED ACTIVATION OF COENZYME B12
    Kenneth Brown; Fiscal Year: 2005
    ..In addition, the possibility that the active species of RTPR is actually a dimer will be investigated by determining the effect of protein concentration on the observed binding constants for AdoCbl and the allosteric effector dGTP. ..
  2. How do Enzymes Generate and Control Free Radicals?
    E Marsh; Fiscal Year: 2007
    ..We will explore the substrate specificity of the enzyme to try and identify mechanism-based inhibitors of the protein and to evaluate the potential of the enzyme to detoxify various aromatic compounds. ..
  3. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  4. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  5. ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER
    Kurt Warncke; Fiscal Year: 2003
    ..A sustained high impact of the EPR spectrometer on biomedicine is therefore anticipated. ..
  6. Co(II)-Radical Pair Dynamics in B12 Enzyme Catalysis
    Kurt Warncke; Fiscal Year: 2008
    ..abstract_text> ..
  7. GENE-NUTRIENT INTERACTIONS IN REDOX HOMEOSTASIS
    Ruma Banerjee; Fiscal Year: 2002
    ....
  8. Gordon Research Sponsored Conference on Cobalamins
    Ruma Banerjee; Fiscal Year: 2003
    ....
  9. SPECTROSCOPIC STUDIES OF ENZYME/SUBSTRATE COMPLEXES
    George Reed; Fiscal Year: 2007
    ..Genes for the enzyme from different species of bacteria will be cloned and over expressed in E. coli, and the proteins subjected to screens for crystallization. ..
  10. REGULATION OF FOLATE METABOLISM
    ROWENA MATTHEWS; Fiscal Year: 2008
    ..We predict that phosphorylation will play an important role in modulating the flux of one-carbon units. ..
  11. SPECTROSCOPIC STUDIES OF ENZYME/SUBSTRATE COMPLEXES
    George Reed; Fiscal Year: 2007
    ..The transient-phase kinetic approach with isotopes will provide new insight into the mechanism of the hydrogen atom abstraction steps in the catalytic cycle. ..
  12. B12 Biosysthesis and Anaerobic Metabolism in Salmonella
    JORGE ESCALANTE; Fiscal Year: 2009
    ..Lastly, we will begin to dissect the biosynthetic pathways for the lower ligand base. One pathway is largely non-enzymatic while the other one is a piecemeal pathway. ..
  13. FOOD FOLATE FORTIFICATION EFFECT ON FOLATE STATUS
    Jacob Selhub; Fiscal Year: 2001
    ..abstract_text> ..
  14. Inducible NOS as a Treatment Target in CLL
    J Weinberg; Fiscal Year: 2005
    ....
  15. Measurement of folate in fortified cereal grain products
    Jacob Selhub; Fiscal Year: 2003
    ..In order to assess the impact of folic acid fortification, it is necessary to have valid data on the actual synthetic folic acid content of fortified foods. ..
  16. TRNA: STUDIES OF PRECURSORS, MUTANTS AND FUNCTIONS
    Sidney Altman; Fiscal Year: 2003
    ....
  17. 14th International Congress on Flavins and Flavoproteins
    ROWENA MATTHEWS; Fiscal Year: 2002
    ..Tea will be served at each poster session. Presentations were selected by an International Organizing Committee with an eye to a diverse representation of speakers at the meeting. ..
  18. Summer Institute Preparation for Careers in Microbiology
    JORGE ESCALANTE; Fiscal Year: 2002
    ..The Institute is the first and only program of its kind providing students with hands-on, practical training in communications and teaching, ethics, grant preparation and review. ..
  19. Vitamin B12 and Folate in Relation to Cognition in the Framingham Offspring Study
    Jacob Selhub; Fiscal Year: 2010
    ..5 About 3-5 percent of seniors have low circulating vitamin B12 levels;6 however, about 20 percent of seniors are found to be deficient when those with elevated methylmalonic acid levels are included. ..