immune sera


Summary: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.

Top Publications

  1. pmc Recombinant dengue type 2 viruses with altered e protein domain III epitopes are efficiently neutralized by human immune sera
    Wahala M P B Wahala
    Address correspondence to Aravinda M de Silva
    J Virol 86:4019-23. 2012
  2. pmc A computational framework for influenza antigenic cartography
    Zhipeng Cai
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA
    PLoS Comput Biol 6:e1000949. 2010
  3. pmc Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants
    Jan Ter Meulen
    Crucell Holland B V, Leiden, Netherlands
    PLoS Med 3:e237. 2006
  4. pmc N-linked glycosylation of the V3 loop and the immunologically silent face of gp120 protects human immunodeficiency virus type 1 SF162 from neutralization by anti-gp120 and anti-gp41 antibodies
    Ruth A McCaffrey
    Seattle Biomedical Research Institute Department of Pathobiology, University of Washington, Seattle, Washington 98109, USA
    J Virol 78:3279-95. 2004
  5. pmc Characterizing anti-HIV monoclonal antibodies and immune sera by defining the mechanism of neutralization
    Emma T Crooks
    Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121, USA
    Hum Antibodies 14:101-13. 2005
  6. pmc Characterization of two novel cryptococcal mannoproteins recognized by immune sera
    Carmelo Biondo
    Department of Pathology and Experimental Microbiology, University of Messina, Messina, Italy
    Infect Immun 73:7348-55. 2005
  7. ncbi Active and passive immunization against Acinetobacter baumannii using an inactivated whole cell vaccine
    Michael J McConnell
    Unit of Infectious Disease, Microbiology, and Preventive Medicine, Institute of Biomedicine of Sevilla, University Hospital Virgen del Rocío CSIC University of Sevilla, 41013 Sevilla, Spain
    Vaccine 29:1-5. 2010
  8. pmc Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes
    Matthias Mueller
    Institute of Virology and Immunoprophylaxis IVI, Mittelhaeusern, Switzerland
    PLoS ONE 5:e9097. 2010
  9. ncbi Purification and characterization of a bovine pregnancy-associated glycoprotein
    A P Zoli
    , Bruxelles, Belgique
    Biol Reprod 45:1-10. 1991
  10. ncbi Taenia solium: a two-dimensional Western blotting method combined with the use of an EST-library for the identification of immunogenic proteins recognized by sera from neurocysticercosis patients
    Fernando Salazar-Anton
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, P O Box 280, SE 171 77 Stockholm, Sweden
    Exp Parasitol 128:371-6. 2011

Detail Information

Publications246 found, 100 shown here

  1. pmc Recombinant dengue type 2 viruses with altered e protein domain III epitopes are efficiently neutralized by human immune sera
    Wahala M P B Wahala
    Address correspondence to Aravinda M de Silva
    J Virol 86:4019-23. 2012
    ..Using recombinant dengue serotype 2 viruses with altered EDIII epitopes, we demonstrate that EDIII epitopes are not the main target of human neutralizing antibody...
  2. pmc A computational framework for influenza antigenic cartography
    Zhipeng Cai
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA
    PLoS Comput Biol 6:e1000949. 2010
    ..The webserver is available at
  3. pmc Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants
    Jan Ter Meulen
    Crucell Holland B V, Leiden, Netherlands
    PLoS Med 3:e237. 2006
    ..Combinations of virus-neutralizing, noncompeting mAbs may have these properties...
  4. pmc N-linked glycosylation of the V3 loop and the immunologically silent face of gp120 protects human immunodeficiency virus type 1 SF162 from neutralization by anti-gp120 and anti-gp41 antibodies
    Ruth A McCaffrey
    Seattle Biomedical Research Institute Department of Pathobiology, University of Washington, Seattle, Washington 98109, USA
    J Virol 78:3279-95. 2004
    ..Collectively, our data support the proposal that the glycosylation and structure of the immunologically silent face of the HIV envelope plays an important role in defining the neutralization phenotype of HIV type 1...
  5. pmc Characterizing anti-HIV monoclonal antibodies and immune sera by defining the mechanism of neutralization
    Emma T Crooks
    Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121, USA
    Hum Antibodies 14:101-13. 2005
    ..We conclude that these methods, together with other mapping approaches, may provide a better understanding of neutralization that could be useful in vaccine research...
  6. pmc Characterization of two novel cryptococcal mannoproteins recognized by immune sera
    Carmelo Biondo
    Department of Pathology and Experimental Microbiology, University of Messina, Messina, Italy
    Infect Immun 73:7348-55. 2005
    ..In conclusion, we identified two novel MPs that are important targets of antibody responses during cryptococcosis. These data may be useful to devise alternative immunity-based strategies to control the disease...
  7. ncbi Active and passive immunization against Acinetobacter baumannii using an inactivated whole cell vaccine
    Michael J McConnell
    Unit of Infectious Disease, Microbiology, and Preventive Medicine, Institute of Biomedicine of Sevilla, University Hospital Virgen del Rocío CSIC University of Sevilla, 41013 Sevilla, Spain
    Vaccine 29:1-5. 2010
    ..These results demonstrate that active and passive immunization using an inactivated whole cell vaccine may be an effective approach for preventing infection by A. baumannii...
  8. pmc Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes
    Matthias Mueller
    Institute of Virology and Immunoprophylaxis IVI, Mittelhaeusern, Switzerland
    PLoS ONE 5:e9097. 2010
    ..Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA...
  9. ncbi Purification and characterization of a bovine pregnancy-associated glycoprotein
    A P Zoli
    , Bruxelles, Belgique
    Biol Reprod 45:1-10. 1991
    ..According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942)...
  10. ncbi Taenia solium: a two-dimensional Western blotting method combined with the use of an EST-library for the identification of immunogenic proteins recognized by sera from neurocysticercosis patients
    Fernando Salazar-Anton
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, P O Box 280, SE 171 77 Stockholm, Sweden
    Exp Parasitol 128:371-6. 2011
    ..None of the antigens were recognized by negative control sera. Despite the limited number of serum samples evaluated in this study, the results indicate that Tsol-p27 might be a suitable candidate for diagnosis of human NCC...
  11. ncbi The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13
    J Fukushi
    Department of Medical Biochemistry, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi ku, Fukuoka, Japan
    J Immunol 165:2818-23. 2000
    ..These findings suggest that angiogenesis dependent on IL-4 and IL-13 is mainly mediated through a soluble VCAM-1/alpha4 integrin pathway...
  12. ncbi Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing
    M Pizza
    IRIS, Chiron S p A, Via Fiorentina 1, 53100 Siena, Italy
    Science 287:1816-20. 2000
  13. pmc In LipL32, the major leptospiral lipoprotein, the C terminus is the primary immunogenic domain and mediates interaction with collagen IV and plasma fibronectin
    Pricila Hauk
    Centro de Biotecnologia Laboratório de Bacteriologia, Instituto Butantan, 05503 900 Sao Paulo, SP, Brazil
    Infect Immun 76:2642-50. 2008
    ..Taken together, our results provide evidence that the LipL32 C terminus is recognized early in the course of infection and is the domain responsible for mediating interaction with ECM proteins...
  14. ncbi Cloning of hamster type XVII collagen cDNA, and pathogenesis of anti-type XVII collagen antibody and complement in hamster bullous pemphigoid
    Katsushi Yamamoto
    Division of Oncology and Biology, Genome Research Group, National Institute of Radiological Sciences, Inage Ku, Chiba, Japan
    J Invest Dermatol 118:485-92. 2002
    ..Under these conditions, IgG but not C3 was deposited on the basement membrane. These results strongly suggest that antigen-antibody complexes and complement initiate dermal-epidermal junction separation...
  15. ncbi Immunologic characterization of Plasmodium vivax antigens using Plasmodium cynomolgi liver stage-primed immune sera
    C Yang
    Department of Pathology, Emory University School of Medicine, Atlanta, Georgia
    Am J Trop Med Hyg 51:365-71. 1994
    ..cynomolgi. In the present study, we demonstrate that these anti-P. cynomolgi immune sera recognized P. vivax (Salvador I) antigens...
  16. ncbi Reduction and enhancement of Plasmodium falciparum transmission by endemic human sera
    Mike van der Kolk
    Radboud University Nijmegen Medical Centre, MMB 268, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Int J Parasitol 36:1091-5. 2006
    ..TR but not TE showed a significant association with the presence of serum antibodies against Pfs48/45 and a non-significant trend to the presence of anti-Pfs230 antibodies...
  17. ncbi Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein
    J J H Chu
    Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
    J Gen Virol 86:405-12. 2005
    ..The data also suggest that similar attachment molecule(s) or receptor(s) were used by WNV and Den 2 virus for entry into C6/36 mosquito cells...
  18. ncbi Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis
    Marcello Otake Sato
    Department of Parasitology, Asahikawa Medical College, Asahikawa Hokkaido, Japan
    J Infect Dis 194:1783-90. 2006
    ..In addition, the conservation of the genes encoding Ag1V1 and Ag2 in T. solium worldwide was verified. These results indicate that Rec-Ag1V1/Ag2 has great potential for usefulness in serodiagnosis as an alternative to native antigens...
  19. ncbi Genome-wide variation and identification of vaccine targets in the Plasmodium falciparum genome
    Jianbing Mu
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 39:126-30. 2007
    ..various highly polymorphic loci and genes, some of which encode new antigens that we confirmed using human immune sera. Our collections of genome-wide SNPs ( approximately 65% nonsynonymous) and polymorphic microsatellites and ..
  20. ncbi Detection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA
    Julia Durzynska
    Department of Molecular Virology, Faculty of Biology, University of A Mickiewicz, Poznan, Poznan
    Viral Immunol 23:415-23. 2010
    ..Although HPV virus-like particles are most often used for anti-HPV antibody detection, the ELISA method presented herein is another viable option for use in RRP patients...
  21. pmc Screening of random peptide library of hemagglutinin from pandemic 2009 A(H1N1) influenza virus reveals unexpected antigenically important regions
    Wanghui Xu
    Department of Chemical Engineering, Tsinghua University, Beijing, China
    PLoS ONE 6:e18016. 2011
    ..The scheme used in this study enables a direct mapping of the antigenic regions of viral proteins recognized by antisera, and may be useful for dissecting the antigenic structures of other viral proteins...
  22. pmc Search for potential vaccine candidate open reading frames in the Bacillus anthracis virulence plasmid pXO1: in silico and in vitro screening
    N Ariel
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Infect Immun 70:6817-27. 2002
    ..The latter ORFs are currently being evaluated to determine their vaccine potential...
  23. pmc Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen
    Orit Gat
    Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Infect Immun 74:3987-4001. 2006
    ..We list more than 30 novel B. anthracis immunoreactive virulence-related proteins which could be useful in diagnosis, pathogenesis studies, and future anthrax vaccine development...
  24. ncbi Identification of epitope regions recognized by tumor inhibitory and stimulatory anti-ErbB-2 monoclonal antibodies: implications for vaccine design
    Y L Yip
    School of Medicine, University of New South Wales, Sydney, New South Wales, Australia
    J Immunol 166:5271-8. 2001
  25. ncbi Passive immunotherapy of Bacillus anthracis pulmonary infection in mice with antisera produced by DNA immunization
    John E Herrmann
    Antibody Science, Inc, 80 Webster Street, Worcester, MA 01603, USA
    Vaccine 24:5872-80. 2006
    ..anthracis (Sterne strain) spores. The high titer antisera we produced will enable an immunization strategy to supplement antibiotic therapy for improving the survival of patients with anthrax...
  26. pmc Antibody prophylaxis and therapy against West Nile virus infection in wild-type and immunodeficient mice
    Michael J Engle
    Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 77:12941-9. 2003
    ..Thus, passive transfer of immune antibody improves clinical outcome even after WNV has disseminated into the central nervous system...
  27. ncbi Beta-glucan, a "specific" biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18)
    J Yan
    Division of Experimental Immunology, Department of Pathology, University of Louisville, KY 40292, USA
    J Immunol 163:3045-52. 1999
    ..Therapy fails when tumors lack iC3b, but can be restored by tumor-specific Abs that deposit iC3b onto the tumors...
  28. pmc Bovine papillomavirus type 1 infection is mediated by SNARE syntaxin 18
    Valerie Laniosz
    School of Graduate and Postdoctoral Studies, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Virol 81:7435-48. 2007
    ..These results support the dependence of syntaxin 18 during BPV1 infection and the ability to interfere with infection by targeting the L2-syntaxin 18 interaction and further define the infectious route of BPV1 mediated by the L2 protein...
  29. ncbi Differential reactivity of immune sera from human vaccinees with field strains of eastern equine encephalitis virus
    J M Strizki
    Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia, USA
    Am J Trop Med Hyg 53:564-70. 1995
    ..Most significantly, although human immune sera displayed high neutralizing titers against each of the NA isolates examined, only negligible neutralizing ..
  30. ncbi Investigation of media formulations promoting differential antigen expression by Photobacterium damsela ssp. piscicida and recognition by sea bass, Dicentrarchus labrax (L.), immune sera
    V Bakopoulos
    Division of Genetics, Department of Biology, University of Patras, Patras, Greece
    J Fish Dis 26:1-13. 2003
    ..The ECPs of the pathogen were not good immunogens in their soluble form despite various treatments prior to immunization. The results are discussed with respect to vaccine development...
  31. ncbi LHRH might act as a negative autocrine regulator of proliferation of human ovarian cancer
    G Emons
    Departments of Gynecology and Obstetrics, Georg August University, Robert Koch Street 40, D 37075, Gottingen, Germany
    Eur J Endocrinol 142:665-70. 2000
    ..The proliferation of human ovarian cancer cell lines is reduced by both LHRH agonists and antagonists. This study was designed to further clarify the possible biological function of this LHRH system...
  32. pmc Bioinformatics and multiepitope DNA immunization to design rational snake antivenom
    Simon C Wagstaff
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom
    PLoS Med 3:e184. 2006
    ..Here we exploit recent molecular sequence analysis and DNA immunization tools to design more rational toxin-targeted antivenom...
  33. ncbi Efficacy of immune sera from human immunoglobulin transgenic mice immunized with a peptide mimotope of Cryptococcus neoformans glucuronoxylomannan
    Robert W Maitta
    Departments of Microbiology and Immunology, Room 709 Forchheimer Bldg, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Vaccine 22:4062-8. 2004
    ..Our findings suggest that GXM-specific human IgM may be a functional mediator of protection against C. neoformans...
  34. ncbi Preparation and functional properties of polyclonal and monoclonal antibodies to murine MD-1
    S Hadidi
    Transplant Research Division, CCRW 2-855 The Toronto Hospital, 200 Elizabeth Street, Ont, M5G 2C4, Toronto, Canada
    Immunol Lett 77:97-103. 2001
  35. pmc Human autoantibodies reveal titin as a chromosomal protein
    C Machado
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2196, USA
    J Cell Biol 141:321-33. 1998
    ..The identification of titin as a chromosomal component provides a molecular basis for chromosome structure and elasticity...
  36. ncbi 13-valent pneumococcal conjugate vaccine immune sera protects against pneumococcal serotype 1, 3, and 5 bacteremia in a neonatal rat challenge model
    Philip Fernsten
    Pfizer Vaccine Research, Pearl River, NY, USA
    Hum Vaccin 7:75-84. 2011
    ..The functional activity of PCV13 immune sera against a virulent type 3 strain was further evaluated using sera from human children immunized with 4 doses of ..
  37. pmc Identification of Bacillus anthracis spore component antigens conserved across diverse Bacillus cereus sensu lato strains
    Sanghamitra Mukhopadhyay
    Biological Defense Research Directorate, Naval Medical Research Center, Rockville, Maryland 20852, USA
    Mol Cell Proteomics 8:1174-91. 2009 of 192 of those candidate genes were expressed and purified in vitro, 75 of which reacted with the rabbit immune sera generated against B. anthracis spores...
  38. ncbi Deciphering epitope specificities within polyserum using affinity selection of random peptides and a novel algorithm based on pattern recognition theory
    Dimitri A Denisov
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    Mol Immunol 46:429-36. 2009
    ..This novel algorithm provides the basis for further development of methods to characterize the breadth of epitope recognition within a complex pool of antibodies...
  39. ncbi Antigenic relationships and relative immunogenicities of isolated metalloproteinases from Echis ocellatus venom
    J M Howes
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
    Toxicon 45:677-80. 2005
    ..This could indicate that antivenoms may lack antibodies to potentially important venom components...
  40. ncbi Preparation of an antibody recognizing both human and rodent MRP1
    C Fernetti
    CSF and Department of BBCM, Centro Servizi Polivalenti d Ateneo, University of Trieste, Via Giorgieri 1, Trieste, 34127, Italy
    Biochem Biophys Res Commun 288:1064-8. 2001
    ..These data indicate that MRP1-A23 allows specific MRP1 detection in both human and rodent tissues and may provide an important tool in the study of MRP1 expression and function in both experimental and clinical materials...
  41. ncbi Isolation and characterization of a human sperm antigen gene h-Sp-1
    Ri Ichiro Kanazawa
    Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
    Int J Androl 26:226-35. 2003
    ..These findings suggest that the h-Sp-1 molecule is expressed in sperm and testes and plays a role in fertilization...
  42. ncbi The molecular basis of the antigenic cross-reactivity between measles and cowpea mosaic viruses
    Wieslawa Olszewska
    Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    Virology 310:183-9. 2003
    ..Passive transfer of anti-wtCPMV antibodies into BALB/c mice conferred partial protection against measles virus induced encephalitis. The results are discussed in the context of cross-protection...
  43. ncbi [Immunogenetic properties of peptides mimicking a human immunodeficiency virus gp41 (HIV-1) epitope recognized by virus-neutralizing antibody 2F5]
    O Iu Tumanova
    Institute of Bioengineering, Vector State Research Center of Virology and Biotechnology, Kol tsovo, Novosibirsk Oblast, 630559 Russia
    Mol Biol (Mosk) 37:556-60. 2003
    ..Rabbits and mice were immunized with the peptides exposed on the surface of filamentous bacteriophages. Antibodies to gp41 were detected in the sera of immunized animals. The virus-neutralizing activity of the sera was examined...
  44. ncbi Toxoplasma gondii: analysis of the active site insertion of its ferredoxin-NADP(+)-reductase by peptide-specific antibodies and homology-based modeling
    Anika Bednarek
    FB Biologie Parasitologie, Philipps Universitat Marburg, Karl von Frisch Str, D 35032, Marburg, Germany
    Exp Parasitol 103:68-77. 2003
    ..Collectively, these data suggest that the insertion in T. gondii FNR does not affect the overall structure of the protein but may have an effect on the binding dynamics of FAD, NADP(+), and/or ferredoxin to FNR...
  45. pmc Isolation of proliferation factor of immature T-cell clone in concanavalin A-stimulated splenocyte culture supernatant
    Yasuhiro Kohama
    Department of Immunology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    Immunology 109:209-16. 2003
    ..A Hitrap-saposin A antibody column bound NPF and pulled down the NPF activity in TCGF. Thus, NPF in TCGF was a saposin A-like protein possessing the capacity for growth and differentiation of immature thymocytes...
  46. ncbi Characterisation of monoclonal antibodies against haemagglutinin associated with Clostridium botulinum type C neurotoxin
    Nazira Mahmut
    Department of Bacteriology, Okayama University Graduate School of Medicine, Japan
    J Med Microbiol 51:286-94. 2002
  47. pmc Expression, immunolocalization and serodiagnostic value of a myophilin-like protein from Schistosoma japonicum
    Hailin Peng
    Shanghai Institute of Hematology, Ruijin Hospital, State Key Laboratory of Medical Genomics, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Exp Parasitol 119:117-24. 2008
    ..4%. Taken together, the myophilin-like protein may prove useful for monitoring the therapeutic effect of praziquantel rather than in serodiagnosis of schistosomiasis...
  48. ncbi The spotted wolffish (Anarhichas minor Olafsen) complement component C3: isolation, characterisation and tissue distribution
    Torill K Abelseth
    Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, N9037 Tromsø, Norway
    Fish Shellfish Immunol 15:13-27. 2003
    ..Immunohistochemical experiments showed that liver hepatocytes and blood contained C3, and in situ hybridisation experiments revealed that liver hepatocytes expressed C3...
  49. ncbi Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica
    I Hochel
    Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague, Czechia
    Folia Microbiol (Praha) 52:511-8. 2007
    ..The prepared antibodies were used in the development of indirect competitive ELISA. At the optimum concentration of the immunoreactants the detection limits were within the range of 3-7 x 10(6) colony-forming units per mL...
  50. ncbi Molecular and functional characterization of the rabbit epididymal secretory protein 52, REP52
    Brett Nixon
    School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 78:910-20. 2008
  51. ncbi Immunization with a tubulin-rich preparation from Trypanosoma brucei confers broad protection against African trypanosomosis
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda
    Exp Parasitol 102:9-22. 2002
    ..These data suggest that tubulin is the relevant immunogen in the preparation used and could therefore be a promising target for the development of a parasite-specific, broad spectrum vaccine...
  52. ncbi Generation and selection of immunized Fab phage display library against human B cell lymphoma
    Yongmei Shen
    Department of Radioimmunoassay, Second Affiliated Hospital of Soochow University, Suzhou, China
    Cell Res 17:650-60. 2007
    ..These immunized Fab antibodies may provide a valuable tool for further study of B cell lymphoma and could also contribute to the improvement of disease therapy...
  53. ncbi Postnatal development and testosterone dependence of a rat epididymal protein identified by neonatal tolerization
    S A Joshi
    Hybridoma Division, National Institute for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, India
    Reproduction 125:495-507. 2003
    ..The importance of epididymis-specific proteins that are regulated by androgens in sperm maturation is discussed, and the need to ascertain the sequence of the protein and clone the cognate gene is indicated...
  54. ncbi Antibodies raised in animals against the Streptococcus agalactiae proteins c alpha and R4 and normal human serum antibodies target distinct epitopes
    Sylvester R Moyo
    Department of Medical Microbiology, Faculty of Medicine, University of Zimbabwe Medical School, PO Box A178, Avondale, Harare, Zimbabwe
    J Med Microbiol 52:379-83. 2003
    ..These findings are important for further clarification of GBS immunology and immunoprotection in humans...
  55. pmc A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas' disease is due to a single parasite lineage
    Javier M Di Noia
    Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus IIB INTECH, Universidad Nacional de General San Martin CONICET, Av General Paz y Albarellos, San Martin, 1650 Buenos Aires, Argentina
    J Exp Med 195:401-13. 2002
    ..The implications of these results for the future research on Chagas' disease could be envisaged...
  56. pmc Antibodies against a synthetic peptide of SagA neutralize the cytolytic activity of streptolysin S from group A streptococci
    James B Dale
    Department of Veterans Affairs Medical Center and Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38104, USA
    Infect Immun 70:2166-70. 2002
    ..Our data also provide convincing evidence that the sagA gene actually encodes the SLS peptide of GAS. The synthetic peptide may prove to be an important component of vaccines designed to prevent GAS infections...
  57. ncbi Trypanosoma brucei: anti-tubulin antibodies specifically inhibit trypanosome growth in culture
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, P O Box 7062, Kampala, Uganda
    Exp Parasitol 102:134-42. 2002
    ..This suggests that the variant surface glycoproteins (VSG) played no part in the generation of these inhibitory antibodies...
  58. ncbi Molecular cloning of a member of the Fasciola hepatica saposin-like protein family
    Ana M Espino
    Laboratory of Parasite Immunology and Pathology, Department of Pathology and Laboratory Medicine Suite 617 A, University of Puerto Rico School of Medicine, P O Box 365067, San Juan, PR 00936 5067, USA
    J Parasitol 89:545-52. 2003
    ..hepatica parasites. rFhSAP-2 also exhibits a strong lytic activity on human erythrocytes and peripheral blood mononuclear cells. This suggests that cell lysis could be 1 of the biological functions of this protein...
  59. ncbi Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera induced by immunizing rabbits with synthetic peptides representing segments of the HPV16 minor capsid protein L2 surface region
    Kazunari Kondo
    Center for Pathogen Genomics, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Virology 358:266-72. 2007
    ..The cross-neutralization appears to be correlated with conserved aa sequences among HPV types. The data in this study provide a basis for designing vaccine antigens effective against a broader spectrum of the high-risk HPVs...
  60. ncbi Anti-capsular antibodies activate killing of Escherichia coli O8:K87 by the alternate complement pathway in porcine serum
    N M Clark
    Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Fair Street and East Campus Loop, Lincoln, NE 68583 0905, USA
    Vet Immunol Immunopathol 114:185-91. 2006
    ..In all cases, Mg-EGTA-treated serum was as effective as non-treated serum in killing, suggesting that bactericidal activity was mediated predominantly if not exclusively via the alternative C pathway...
  61. ncbi Development of an enzyme-linked immunosorbent assay for toosendanin
    Jing Zhang
    R and D Center of Biorational Pesticides, Northwest A and F University, 22 Xinong Road, Yangling, Shaanxi 712100, China
    Anal Chim Acta 622:182-8. 2008
    ..3%, respectively. Linear regression analysis showed good correlation between the TSN concentrations derived from ELISA and HPLC analyses, which suggested that the ELISA is a convenient supplementary analytical tool for monitoring TSN...
  62. ncbi Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis
    Elizabeth Ferrer
    Instituto de Salud Carlos III, Centro Nacional de Microbiologia, Majadahonda, Madrid, Spain
    Exp Parasitol 110:1-11. 2005
    ..solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed...
  63. ncbi Antiserum specific for the intact isoform-3 of metallothionein
    Abigail M Tokheim
    Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, United States
    J Biochem Biophys Methods 63:43-52. 2005
    ..The concentration of MT3 was found to be 3.0+/-0.8 microg/ml or approximately 3.5 microg/g mouse brain (wet weight)...
  64. ncbi An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus
    D E Webster
    Botany Department, University of Otago, Dunedin, New Zealand
    Arch Virol 150:1921-6. 2005
    ..The PAb-cp3'Delta antiserum is specific for RGMV and recognises RGMV strains from each major phylogenetic cluster. PAb-cp3'Delta may be used in ELISAs for fast, accurate and inexpensive detection of RGMV...
  65. ncbi Vaccine potential of the Neisseria meningitidis lactoferrin-binding proteins LbpA and LbpB
    Annika Pettersson
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Vaccine 24:3545-57. 2006
    ..Our results show that both proteins are immunogenic and able to induce bactericidal antibodies, but that the cross-reactivity of these antibodies is limited...
  66. ncbi Purification and serological analyses of tospoviral nucleocapsid proteins expressed by Zucchini yellow mosaic virus vector in squash
    Tsung Chi Chen
    Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan
    J Virol Methods 129:113-24. 2005
    ..The procedure provides a convenient and fast way for production of large quantities of pure NPs of tospoviruses in planta. The system also has a potential for production of any proteins of interest in cucurbits...
  67. ncbi Immunochemical detection of flucloxacillin adduct formation in livers of treated rats
    Michelle A Carey
    Department of Pharmacology and Therapeutics, University College Cork, Ireland
    Toxicology 216:41-8. 2005
    ..These studies demonstrate for the first time that treatment with flucloxacillin results in the formation of hepatic protein adducts...
  68. pmc A live-attenuated Pseudomonas aeruginosa vaccine elicits outer membrane protein-specific active and passive protection against corneal infection
    Tanweer S Zaidi
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Infect Immun 74:975-83. 2006
    ..aeruginosa corneal infections caused by diverse LPS serogroups...
  69. ncbi Immunogenicity of synthetic peptides derived from Plasmodium falciparum proteins
    Noraida C Zerpa
    Instituto de Estudios Avanzados, Department of Biosciencias, Carretera Hoyo de la Puerta, Caracas, Venezuela
    Exp Parasitol 113:227-34. 2006
    ..Our results confirm the potential value of synthetic peptides when inducing monospecific polyclonal antibodies for the development of diagnostic tests based on the capture of antigens...
  70. ncbi Mimotope of Leptospira from phage-displayed random peptide library is reactive with both monoclonal antibodies and patients' sera
    Rongdej Tungtrakanpoung
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Vet Microbiol 115:54-63. 2006
    ..The results demonstrate that phage display technique has potential for rapidly identifying phage mimotopes that interact with leptospiral MAbs and patient's sera...
  71. ncbi Immunoassay for biochanin A
    Oldrich Lapcik
    Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Technicka 5, 166 28 Praha 6, Czech Republic
    J Immunol Methods 294:155-63. 2004
    ..5% for RIA; 5.0% for ELISA), genistein (0.8% for RIA; 2.8% for ELISA) and formononetin (0.4% for RIA; 0.3% for ELISA). These methods were used for the analysis of biochanin A in alfalfa and in several nonleguminous plants...
  72. pmc A Vibrio cholerae classical TcpA amino acid sequence induces protective antibody that binds an area hypothesized to be important for toxin-coregulated pilus structure
    Ronald K Taylor
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Infect Immun 72:6050-60. 2004
    ..The sequences of TcpA 15 that induce protective antibody were localized on a model of assembled TCP. These sequences are centered on a site that is predicted to be important for TCP structure...
  73. ncbi Characterization of Bothrops jararaca coagulation inhibitor (BjI) and presence of similar protein in plasma of other animals
    Anita M Tanaka-Azevedo
    Laboratorio de Fisiopatologia, Instituto Butantan, Avenida Vital Brazil, 1500, CEP 05503 900 São Paulo SP, Brazil
    Toxicon 44:289-94. 2004
    ..neuwiedi and Crotalus durissus terrificus, and these proteins also prolong thrombin time. Moreover, these results evidence the presence of target enzymes in snake venom for plasma BjI...
  74. ncbi Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific antirabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues
    Satoshi Inoue
    Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan
    Pathol Int 53:525-33. 2003
  75. ncbi Structure and serological characterization of the O-antigen of Proteus mirabilis O18 with a phosphocholine-containing oligosaccharide phosphate repeating unit
    Rafal Fudala
    Institute of Microbiology and Immunology, University of Lodz, 90 237 Lodz, Poland
    Carbohydr Res 338:1835-42. 2003
    ..mirabilis O18 serum showed the importance of the glycosyl phosphate group in manifesting the serological specificity of the O18-antigen...
  76. ncbi Humoral immune responses to peptides derived from the beta-amyloid peptide C-terminal sequence
    David L Miller
    New York State Institute for Basic Research in Developmental Disabilities Staten Island, NY 10314, USA
    Amyloid 14:39-50. 2007
    ..This information is necessary to develop sensitive and specific immunoassays to quantify Abeta isoforms in brain extracts and in body fluids...
  77. ncbi Antigenic profile of human recombinant PrP: generation and characterization of a versatile polyclonal antiserum
    M Sachsamanoglou
    Laboratory of Pharmacology, School of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Thessaloniki 541 24, Greece
    J Neuroimmunol 146:22-32. 2004
    ..Our knowledge concerning immunoprophylaxis against prion diseases and the important role played by conformational changes of PrP is increasing rapidly. The findings reported here should add to this body of knowledge...
  78. ncbi Expression of a foot-and-mouth disease virus immunodominant epitope by a filamentous bacteriophage vector
    Y J Kim
    Laboratoire d Etudes et de Recherches en Pathologie Animale et Zoonoses, AFSSA, Maisons Alfort, France
    Arch Virol 149:365-77. 2004
    ..This suggests a possible role for phage displayed peptides in inducing anti FMDV immunity and the possibility of further development is discussed...
  79. ncbi Development of an enzyme-linked immunosorbent assay for serum 11-dehydrocorticosterone in rat and mouse
    Noriko Tagawa
    Department of Medical Biochemistry, Kobe Pharmaceutical University
    Biol Pharm Bull 30:403-9. 2007
    ..These results verify that the proposed ELISA for 11-dehydrocorticosterone is useful for measuring 11beta-HSD activities in combination with the determination of serum corticosterone in rats and mice...
  80. ncbi Wuchereria bancrofti: cloning and characterization of heat shock protein 70 from the human lymphatic filarial parasite
    V Ravi
    Centre for Biotechnology, Anna University, Chennai, India
    Exp Parasitol 106:1-10. 2004
    ..Both immunodominant self-HSP70 and HSP70 from other microbial infections may be primary targets for developing autoantibodies naturally...
  81. ncbi Aldehyde modification of peptide immunogen enhances protein-reactive antibody response to toxic shock syndrome toxin-1
    Alfonso Bavoso
    Universita degli Studi della Basilicata, Department of Chemistry, Potenza, Italy
    J Pept Sci 12:843-9. 2006
    ..These data suggest that aldehyde modification can boost immunogenicity of peptide-based vaccines, generating epitope-specific immune responses against the cognate protein antigens without using potent adjuvants...
  82. ncbi DNA immunization for the production of monoclonal antibodies to Blo t 11, a paramyosin homolog from Blomia tropicalis
    J D A Ramos
    Department of Paediatrics, National University of Singapore, Singapore
    Allergy 59:539-47. 2004
    ..DNA-based immunization is an attractive alternative strategy that bypasses antigen purification for antibody production...
  83. ncbi Epitope mapping and features of the epitope for monoclonal antibodies inhibiting enzymatic activity of Helicobacter pylori urease
    Ryoji Fujii
    Fukuyama Medical Laboratory Company, Fukuyama City, Hiroshima 720 8510, Japan
    Biotechnol Bioeng 86:434-44. 2004
    ..pylori infecting human gastric mucosa. These results suggest that the above sequence (22 mer) must be an important epitope, although it locates in the alpha-subunit but not in the beta-subunit...
  84. ncbi Lipopolysaccharide O-antigen antibody-based detection of the fish pathogen Flavobacterium psychrophilum
    Elizabeth M Crump
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, Canada
    J Mol Microbiol Biotechnol 6:182-90. 2003
    ..was purified and conjugated to keyhole limpet haemocyanin and bovine serum albumin for the generation of rabbit immune sera and the development of antibody-based diagnostic tests...
  85. ncbi Antisera to polycyclic aromatic hydrocarbons and their application on the detection of chemical carcinogens in blood sera of oncological patients
    Andrey N Glushkov
    Kemerovo Scientific Centre, Siberian Branch of Russian Academy of Sciences, Kemerovo 650610, Russia
    Exp Oncol 26:145-8. 2004
    ..To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure...
  86. pmc The ABC transporter protein OppA provides protection against experimental Yersinia pestis infection
    Mikio Tanabe
    Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 74:3687-91. 2006
    ..When the immunized mice were challenged with Y. pestis, the OppA-immunized mice showed an increased time to death compared to other groups, and protection appeared to correlate with the level of immunoglobulin G antibody to OppA...
  87. ncbi Constitutive tyrosine phosphorylation of the GABA(A) receptor gamma 2 subunit in rat brain
    N J Brandon
    Medical Research Council Laboratory of Molecular Cell Biology and Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK
    Neuropharmacology 41:745-52. 2001
    ..Together our results suggests that tyrosine phosphorylation of the gamma2 subunit, possibly by closely associated Src, may be a dynamic mechanism for regulating GABA(A) receptor function in the brain...
  88. ncbi Isolation and characterization of mouse probasin: An androgen-regulated protein specifically expressed in the differentiated prostate
    M A Johnson
    Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Prostate 43:255-62. 2000
  89. pmc The pspC gene of Streptococcus pneumoniae encodes a polymorphic protein, PspC, which elicits cross-reactive antibodies to PspA and provides immunity to pneumococcal bacteremia
    A Brooks-Walter
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Infect Immun 67:6533-42. 1999
    ..Mice immunized with PspC were protected against challenge with a strain that expressed PspA but not PspC. The PspA- and PspC-cross-reactive antibodies were directed to the proline-rich domain present in both molecules...
  90. ncbi NA14 is a novel nuclear autoantigen with a coiled-coil domain
    F Ramos-Morales
    Departamento de Microbiologia, Facultad de Biologia, Universidad de Sevilla, Spain
    J Biol Chem 273:1634-9. 1998
    ..This novel protein has been termed NA14 for Nuclear Autoantigen of 14 kDa...
  91. pmc Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:3161-70. 2004
    ..leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein...
  92. ncbi Studies on natural killer (NK) cells. I. NK cell specific antibodies in CE anti-CBA serum
    R C Burton
    J Immunol 126:1985-9. 1981
    ..2, and the corresponding antibodies as anti-NK-1.2. However, the possibility that CE anti-CBA serum detects a new system of NK-specific antigen has not been excluded...
  93. ncbi Murine monoclonal anti-idiotypic antibody as a surrogate antigen for human Her-2/neu
    R Baral
    Division of Hematology/Oncology, Department of Internal Medicine and The Barrett Cancer Center for Prevention, Treatment and Research, University of Cincinnati, OH 45267-0509, USA
    Int J Cancer 92:88-95. 2001
    ..The immune sera inhibited binding between Ab1 and Ab2 and vice versa (binding of Ab2 to Ab1), indicating that it was a true ..
  94. ncbi The role of antibodies specific for toxic sPLA2s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom
    Beata Halassy
    Research and Development Department, Institute of Immunology, Inc, Rockefellerova 10, HR 10 000 Zagreb, Croatia
    Comp Biochem Physiol C Toxicol Pharmacol 148:178-83. 2008
    ..We further revealed that Atxs and structurally very similar but non-toxic AtnI2 from the venom are not immuno cross-reactive...
  95. ncbi Recognition of HLA class II molecules by antipeptide antibodies elicited by synthetic peptides selected from regions of HLA-DP antigens
    A Chersi
    Regina Elena Institute for Cancer Research, Rome, Italy
    Z Naturforsch C 42:1313-8. 1987
    ..histocompatibility antigens resulted in the production of specific antibodies that were then isolated from the immune sera by chromatography on Sepharose-peptide immunoadsorbents...
  96. ncbi The localization of histone H3.3 in germ line chromatin of Drosophila males as established with a histone H3.3-specific antiserum
    A Akhmanova
    Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, Nijmegen, The Netherlands
    Chromosoma 106:335-47. 1997
    ..In Drosophila polytene chromosomes, however, a rather uniform distribution of the histone H3.3 was observed. The possible role of histone H3.3 is discussed...
  97. ncbi Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins
    J Liu
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Life Sciences, Chinese Academy of Sciences, Shanghai, 200031, People's Republic of China
    Biotechnol Appl Biochem 34:109-19. 2001
    ..The E2 proteins and rabbit antiserum reported here could find wider applications in the development of effective diagnostic, prophylactic and therapeutic measures against HCV...
  98. ncbi Sensitive and rapid immunoassays for Salmonella enteritidis
    J L Liu
    University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania, USA
    J Clin Lab Anal 15:165-70. 2001
    ..The antibodies that have been made to the unique sugar tyvelose should improve the specificity in assays for S. enteritidis...
  99. ncbi Production and characterization of an antiserum which recognizes the native receptor for thyrotropin-releasing hormone
    F Chen
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Biochem Biophys Res Commun 285:742-50. 2001
    ..These observations are consistent with our previous findings using an epitope-tagged TRHR. In conclusion, we have prepared an antiserum that recognizes the native TRHR by Western blot analysis and confocal microscopy...
  100. ncbi A notable phenomenon recapitulated. A fusion product of a murine lymphoma cell and a leukemia virus-neutralizing antibody-producer host plasma cell formed spontaneously and secreting the specific antibody continuously
    J G Sinkovics
    Cancer Institute, St Joseph s Hospital Department of Medicine, The University of South Florida College of Medicine, Tampa, FL, USA
    Acta Microbiol Immunol Hung 52:1-40. 2005
    ..The growth of #620 or #818 cells in YAS mice treated with rabbit anti-lymphoma cell immune sera was strongly inhibited but culture fluids of #818 cells showed weak and insignificant inhibition against ..
  101. ncbi Growth and cultural characteristics of Calymmatobacterium granulomatis--the aetiological agent of granuloma inguinale (Donovanosis)
    A B Kharsany
    Department of Medical Microbiology, Faculty of Medicine, University of Natal, Durban, South Africa
    J Med Microbiol 46:579-85. 1997 were examined by a rapid Giemsa (RapiDiff) stain and by an indirect immunofluorescence test with immune sera. Representative cultures were examined by transmission electron microscopy. C...

Research Grants62

  1. Development of Breast Cancer Vaccines
    WEI ZEN NONE WEI; Fiscal Year: 2013
    ..Human DR5 immune sera induced in wild type mice triggered apoptosis of TNBC and inhibited their growth in vivo...
  2. Development of a recombinant Nipah virus vaccine for wildlife
    MILOSZ ANDRZEJ FABER; Fiscal Year: 2010
    ..for protection against Nipah virus infection have not been completely defined, passive transfer of monospecific immune sera against the F and G proteins of Nipah virus protects hamsters against a lethal challenge by this virus, ..
  3. Identification of New Antigens for a Plague Vaccine
    Ashok K Chopra; Fiscal Year: 2013
    ..We have identified several immunogenic proteins in the WT CO92 strain that reacted with the immune sera of rats infected with CO92 strain...
  4. Molecular Evolution Strategies to Derive New Recombinant AAV Vectors
    Mark A Kay; Fiscal Year: 2012
    ..selected for capsids that promote AAV infection in human hepatocytes and that are resistant to pooled human immune sera. The first capsid that was vectorized and extensively studied has important biological properties that have ..
  5. Novel Vaccine Immunogens for Pandemic H5 Influenza
    ROBERT GERALD WHALEN; Fiscal Year: 2009
    ..of the H5 HA protein can be created that are more immunogenic than the wild-type proteins, and will induce immune sera with potentially broad neutralization potency...
  6. PspA: A Potential Pneumococcal Vaccine Component
    David E Briles; Fiscal Year: 2012
    ..Relevant surrogate assays are needed to evaluate immune sera from phase I and II human safety and immunogenicity trials...
  7. Optimization of HIV vaccines for the induction of cross-reactive antibodies
    Shan Lu; Fiscal Year: 2013
    ..Aim 4 To examine the epitope profiles in immune sera elicited by DNA prime-protein boost approach and identify the epitope specificities of antibodies responsible ..
  8. Guinea pig cytomegalovirus as a model for vaccines that target endocytic entry
    Michael A McVoy; Fiscal Year: 2010
    ..We found that the neutralizing activities of human immune sera are on average 48-fold more potent in neutralizing HCMV entry into epithelial cells compared to fibroblasts, ..
  9. High throughput screening for anti-adhesion drugs to treat severe malaria
    Andrew V Oleinikov; Fiscal Year: 2010
    ..binding;b) unlabeled receptors;c) antibodies against receptors and against receptor- binding PfEMP1 domains;d) immune sera collected from patients living in malaria endemic areas...
  10. Pure and Authentic HIV-1 Env Immunogens
    James M Binley; Fiscal Year: 2013
    ..A consistent finding of immunogenicity studies has been that while immune sera efficiently bind to the index immunogen, few Abs recognize the native form of Envelope glycoprotein (Env) found ..
  11. High Mannose Type Carbohydrate-based HIV/AIDS Vaccine
    Yu Geng; Fiscal Year: 2009
    ..cerevisiae, identified several yeast glycoproteins that cross-react with 2G12, elicited immune sera that cross-react with Env glycoproteins of HIV-1 and SIV, and neutralized HIV-1 pseudoviruses...
    JONATHAN RAVDIN; Fiscal Year: 1993
    ..the immunogenicity and vaccine efficacy of Cyt proteins and the major amebic antigens recognized by human immune sera by immunization of gerbils with purified B- galactosidase fusion proteins...
  13. Microalgal Oral Vaccines for Control of Amebiasis
    Richard Wagner; Fiscal Year: 2004
    ..specific to the Gal/GalNAc lectin antigen by western blot analysis using preimmune (negative control) and immune sera from vaccinated and non-vaccinated mice...
    David Lyerly; Fiscal Year: 1999
    ..The lectin is a major conserved surface antigen which is recognized by the immune sera of 99 percent of tested patients with amebic liver abscess and colitis...
    Liise Anne Pirofski; Fiscal Year: 2009
    ..neoformans in normal and human immunoglobulin transgenic mice. Immune sera from immunized mice were protective in naive mice...
    Chandler Dawson; Fiscal Year: 1980
    ..p.) conjunctiva will be determined. The effect of adding immune sera, macrophages and T cells will be measured by changes in amount of agent produced...
  17. Pneumococcal conjugate vaccines and old adults
    MOON NAHM; Fiscal Year: 2009
    ..opsonic capacity for one serotype with completely human reagents, and by measuring the capacity of the immune sera to protect mice against pneumococcal infections;1b) by analyzing the sera from a previous study of 5-valent PCV ..
    James Samuel; Fiscal Year: 2003
    ..Additional outer membrane proteins will be evaluated for reactivity with immune sera and major antigens cloned. This will include components of a putative type 4 secretion system...
  19. Adhesins and Invasins of Francisella Tularensis
    Barbara Mann; Fiscal Year: 2004
    ..Specifically we will verify the presence of type IV pili by microscopy and immune sera. We will isolate pili from F. tularensis and sequence the pilin subunit by mass spectrometry analysis...
  20. A Peptide-Based Vaccine to Nicotine
    Sam Sanderson; Fiscal Year: 2004
    ..rats in the absence of added adjuvant and demonstrating: 1) the presence of nicotine-specific Abs in immune sera and 2) an attenuation of nicotine-induced psychoactive effects in two well-established rat models of nicotine ..
    William Picking; Fiscal Year: 2001
    ..IpaC functional domains will be identified using monoclonal antibodies and polyclonal immune sera which recognize defined epitopes and are characterized with respect to inhibitory or stimulatory activities on ..
    Naomi Lang Unnasch; Fiscal Year: 1999
    ..Finally, expression of the P. falciparum alternative oxidase gene in E. coli will be used to test for its activity in this heterologous system and as a first step toward the generation of immune sera to parasite alternative oxidase.
    TOBILI SAM YELLOWE; Fiscal Year: 1999
    ..Moreover, immune sera from individuals in an area hyperendemic for Falciparum malaria reacts with rhoptry proteins by immunoblot and ..
    CHING LI LEE; Fiscal Year: 1980
    ..Specific immune sera are then raised with the purified enzymes in small animals (rabbits or goats)...
  25. Developing Broadly Neutralizing MAbs for an HIV Vaccine
    Yu Geng; Fiscal Year: 2007
    ..2) Prepare gp120-tetanus toxoid conjugates, immunize mice and screen immune sera for cross-reactive antibodies to gp120s from different subtypes, examine inhibition of gp120-DC-SIGN binding, ..
  26. Peptide Vaccines from Neutralizing Antibodies 2F5 & 2G12
    Jamie Scott; Fiscal Year: 2002
    ..Abs following two immunization strategies in mice, XenoMouse animals and macaques; and will then test the immune sera for the presence of HIV-1-binding Abs and for neutralizing activity...
    Michael Murphey Corb; Fiscal Year: 1993
    ..The combined projects will produce and share a broad range of synthetic and recombinant viral components, immune sera and monoclonal antibodies that will enable detailed evaluation of immune responses to vaccines and ..
  28. Development of Peptide-Based Vaccines to Nicotine
    Sam Sanderson; Fiscal Year: 2006
    ..nicotine vaccines in the absence of added adjuvant and demonstrate the presence of nicotine-specific Abs in immune sera/tissues and an attenuation of nicotine-induced behavioral effects in two well-established rat models of ..
    C Bell; Fiscal Year: 1980
    ..involves planned immunization of rabbits and monkeys with human lung cancer plasma membranes and assay of the immune sera by indirect immunofluorescence assay...
    Opendra Narayan; Fiscal Year: 1990
    ..Using various substrates of virus, virus-infected cells and deglycosylated virus-infected cells to absorb immune sera; we will determine whether virus surface carbohydrates are the major epitopes in virus neutralization...
    Jamie Scott; Fiscal Year: 1999
    ..the production of HIV-1-cross-reactive antibodies by a number of immunization strategies, and will then test immune sera for the presence of HIV-1-binding antibodies and for neutralizing activity...
  32. Immunoprevention of H. pylori gastritis
    JOSEPH CUMMINS; Fiscal Year: 2001
    ..Protection will be correlated to patterns of Western blot immunoreactivity of convalescent immune sera and isolated leukocyte in vitro responses to putative bacterial antigen(s)...
  33. Immunogenicity of the Membrane-Proximal Region of HIV-1 gp41
    Jamie Scott; Fiscal Year: 2007
    ..DNA encoding the most promising of these gp41 constructs to immunize rabbits; and (iii) to test the resulting immune sera for their ability to neutralize HIV-1 primary isolates, and to determine if these Nt reactivities are specific ..
    Lynn Romrell; Fiscal Year: 1980
    ..The isolated proteins can also be used to determine the presence of antibodies in auto-immune sera specific to these proteins...
    ROBERT REESE; Fiscal Year: 1980
    b>Immune sera from humans, monkeys and rabbits have been studied using immunofluorescence, immunoelectron microscopy, SDS acrylamide gel electrophoresis and the inhibition of the growth of Plasmodium falciparum in culture...
    Soldano Ferrone; Fiscal Year: 1999
    ..Rabbit immune sera will be tested for their ability to mediate immune lysis of human melanoma cells with differential expression ..
    CHARLES METZ; Fiscal Year: 1980
    ..2) Immune sera from the infertile rabbits are presumed to contain antibodies against "antifertility" antigens of sperm...
  38. Eliciting neutralizing antibodies against the HCV E2 envelope glycoprotein
    Tatjana Dragic; Fiscal Year: 2009
    ..The resulting immune sera will be characterized for potency and breadth of neutralization...
  39. 5th Comparative Medicine Resource Center Director Mtg.
    Stephen Barthold; Fiscal Year: 2008
  40. Recombinant archaeal organelles of SIV and HIV antigens
    Elizabeth Stuart; Fiscal Year: 2002
    Arturo Casadevall; Fiscal Year: 2006
    ..These studies are expected to yield new insights on fundamental aspects of antibody function as well as practical information for the development of second generation antibody reagents for human therapy. ..
    Stephen Barthold; Fiscal Year: 2004
    ..abstract_text> ..
  43. Design and Use of HIV V3 Mimetic Immunogens
    Susan Zolla Pazner; Fiscal Year: 2008
    ..Finally, in Aim 3, we will examine how the intra- and inter-molecular interactions of gpl20 and gp41 affect neutralization sensitivity to anti-V3 mAbs. ..
    Stephen Barthold; Fiscal Year: 2003
  45. Mining slime mold for susceptibility genes to fungi
    Arturo Casadevall; Fiscal Year: 2005
    ..discoideum system will lead to the identification of genetic pathways that are not immediately obvious from standard fungal-macrophage experiments. ..
  46. A novel Dlg isoform and identification of its interacting proteins
    Kyung Ok Cho; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  47. Surgical Adhesions: Role of T cells in Pathogenesis
    Arthur Tzianabos; Fiscal Year: 2005
    ..abstract_text> ..
  48. Mycobacterium avium subspecies in Crohn's Disease
    Saleh Naser; Fiscal Year: 2005
    ..Ultimately, the outcome of this study will go a long way toward clearing up the MAP debate. ..
    Abhiram Sahu; Fiscal Year: 2001
    ..These studies will further our understanding of leptin signaling in the hypothalamus in relation to feeding, and therefore will be relevant to the development of therapeutic approaches to eating disorders. ..
    DENNIS METZGER; Fiscal Year: 2001
    ..The results will provide important insight into the mechanisms responsible for successful xenogeneic transplantation and could lead to targeted cytokine therapies to prevent tissue rejection. ..
    Theoharis Theoharides; Fiscal Year: 2001
    ..They may also help explain some key events in the pathophysiology of migraines, the clinical symptoms of which are often precipitated by stress. ..
    Aravinda de Silva; Fiscal Year: 2002
    ..This study will provide insight into tick-borne transmission of bacterial pathogens and lead to novel strategies for blocking transmission and preventing Lyme arthritis. ..
    LYNDA MORRISON; Fiscal Year: 2002
    ..This information will facilitate developent of immunization strategies against HSV and possibly other pathogens of the genital tract. ..
  54. Peptidergic Neurotransmission in the Spinal Cord
    VIRGINIA SEYBOLD; Fiscal Year: 2009
    ..If the hypothesis is proven to be true, the data will provide evidence for a new therapeutic strategy that may be effective in treating peripheral inflammatory diseases. ..
    Pawel Goluszko; Fiscal Year: 2002
    ..The investigators propose to evaluate in three Specific Aims the general hypothesis that cross-linking of DAF on human monocytes/macrophages by E. coli bearing Dr fimbriae inhibits IL-2 production. ..
    LEON TSENG; Fiscal Year: 2002
    ..The tail-flick and hot-plate tests will be used to determine antinociceptive responsiveness of mice or/and rats in this research. These studies will provide valuable insight into the cellular mechanisms of mu opioid antinociception. ..
  57. Host Response in S. aureus Infections: Role of T cells
    Arthur Tzianabos; Fiscal Year: 2006
    ..aureus infections. This information may reveal new strategies for the prevention or treatment of S. aureus infections through immunomodulation of the host response to this organism. ..
  58. Mechanisms of Leptin Signaling in the Hypothalamus
    Abhiram Sahu; Fiscal Year: 2007
    ..These studies will further our understanding of leptin signaling in the hypothalamus in relation to feeding, and therefore will be relevant to the development of therapeutic approaches to eating disorders. [unreadable] [unreadable]..
    Carl Nathan; Fiscal Year: 2004
    ..abstract_text> ..
  60. Transcriptionally Active PCR to Improve DNA Vaccines
    David Roth; Fiscal Year: 2004
    ..A high throughput vaccine antigen screening system will be developed to enable the identification of potent vaccine antigens from complex organisms such as malaria. ..