mutagens

Summary

Summary: Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.

Top Publications

  1. ncbi Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing
    R R Tice
    Integrated Laboratory Systems, Research Triangle Park, North Carolina 27709, USA
    Environ Mol Mutagen 35:206-21. 2000
  2. ncbi The comet assay for DNA damage and repair: principles, applications, and limitations
    Andrew R Collins
    Department of Nutrition, University of Oslo, PO Box 1046 Blindern, N 0316 Oslo, Norway
    Mol Biotechnol 26:249-61. 2004
  3. ncbi Cytokinesis-block micronucleus cytome assay
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 10041, Adelaide 5000, South Australia, Australia
    Nat Protoc 2:1084-104. 2007
  4. ncbi Escherichia coli induces DNA double-strand breaks in eukaryotic cells
    Jean Philippe Nougayrede
    INRA, UMR1225, Ecole Nationale Veterinaire de Toulouse, Toulouse F 31076, France
    Science 313:848-51. 2006
  5. pmc Escherichia coli induces DNA damage in vivo and triggers genomic instability in mammalian cells
    Gabriel Cuevas-Ramos
    Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1225, F 31076 Toulouse, France
    Proc Natl Acad Sci U S A 107:11537-42. 2010
  6. ncbi Genotoxicity of silver nanoparticles in Allium cepa
    Mamta Kumari
    Nanobio Medicine Group, School of Bio Sciences and Technology, VIT University, Vellore 632014, India
    Sci Total Environ 407:5243-6. 2009
  7. ncbi The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test
    A C Bulmer
    School of Human Movement Studies, University of Queensland, Brisbane 4072, Australia
    Mutat Res 629:122-32. 2007
  8. ncbi Dose-response and operational thresholds/NOAELs for in vitro mutagenic effects from DNA-reactive mutagens, MMS and MNU
    Lynn H Pottenger
    Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, MI 48674, USA
    Mutat Res 678:138-47. 2009
  9. ncbi Chemoprotective effects of Zataria multiflora against genotoxicity induced by cyclophosphamide in mice bone marrow cells
    Seyed Jalal Hosseinimehr
    Department of Radiopharmacy, Faculty of Pharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran
    Integr Cancer Ther 9:219-23. 2010
  10. ncbi Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells
    Amirhosein Ahmadi
    Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
    Arch Pharm Res 31:794-7. 2008

Detail Information

Publications360 found, 100 shown here

  1. ncbi Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing
    R R Tice
    Integrated Laboratory Systems, Research Triangle Park, North Carolina 27709, USA
    Environ Mol Mutagen 35:206-21. 2000
    ....
  2. ncbi The comet assay for DNA damage and repair: principles, applications, and limitations
    Andrew R Collins
    Department of Nutrition, University of Oslo, PO Box 1046 Blindern, N 0316 Oslo, Norway
    Mol Biotechnol 26:249-61. 2004
    ..Alternatively, the repair activity in a cell extract can be measured by incubating it with nucleoids containing specific damage...
  3. ncbi Cytokinesis-block micronucleus cytome assay
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 10041, Adelaide 5000, South Australia, Australia
    Nat Protoc 2:1084-104. 2007
    ..The procedure can take up to 5 days to complete...
  4. ncbi Escherichia coli induces DNA double-strand breaks in eukaryotic cells
    Jean Philippe Nougayrede
    INRA, UMR1225, Ecole Nationale Veterinaire de Toulouse, Toulouse F 31076, France
    Science 313:848-51. 2006
    ..Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications...
  5. pmc Escherichia coli induces DNA damage in vivo and triggers genomic instability in mammalian cells
    Gabriel Cuevas-Ramos
    Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1225, F 31076 Toulouse, France
    Proc Natl Acad Sci U S A 107:11537-42. 2010
    ..Therefore, colon colonization with these E. coli strains harboring the pks island could contribute to the development of sporadic colorectal cancer...
  6. ncbi Genotoxicity of silver nanoparticles in Allium cepa
    Mamta Kumari
    Nanobio Medicine Group, School of Bio Sciences and Technology, VIT University, Vellore 632014, India
    Sci Total Environ 407:5243-6. 2009
    ..The findings also suggest that plants as an important component of the ecosystems need to be included when evaluating the overall toxicological impact of the nanoparticles in the environment...
  7. ncbi The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test
    A C Bulmer
    School of Human Movement Studies, University of Queensland, Brisbane 4072, Australia
    Mutat Res 629:122-32. 2007
    ..01-2 micromol/plate) in the presence of three bacterial strains (TA98, TA100, TA102). A variety of mutagens including benzo[alpha]pyrene (B[alpha]P), 2,4,7 trinitrofluorenone (TNFone), 2-aminofluorene (2-AF), sodium azide ..
  8. ncbi Dose-response and operational thresholds/NOAELs for in vitro mutagenic effects from DNA-reactive mutagens, MMS and MNU
    Lynn H Pottenger
    Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, MI 48674, USA
    Mutat Res 678:138-47. 2009
    ....
  9. ncbi Chemoprotective effects of Zataria multiflora against genotoxicity induced by cyclophosphamide in mice bone marrow cells
    Seyed Jalal Hosseinimehr
    Department of Radiopharmacy, Faculty of Pharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran
    Integr Cancer Ther 9:219-23. 2010
    ..It appeared that ZM with antioxidative activity reduced the oxidative stress and genotoxicity induced by CP in mouse bone marrow cells...
  10. ncbi Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells
    Amirhosein Ahmadi
    Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
    Arch Pharm Res 31:794-7. 2008
    ..It is obvious that hesperidin, may with antioxidative activity, reduced the oxidative stress and genotoxicity induced by cyclophosphamide in mouse bone marrow cells...
  11. ncbi Mutagens and carcinogens in size-classified air particulates of a northern Italian town
    S Monarca
    Chair of Hygiene and Preventive Odontoiatry, Medicine Faculty, University of Brescia, Italy
    Sci Total Environ 205:137-44. 1997
    ..5 micron) particles, which accounted for most of PAHs and mutagenicity. A similar pattern of distribution was found for heavy metals, which were relatively more abundant in small (< 1.5 microns) particles compared to coarser ones...
  12. ncbi The protein degradation response of Saccharomyces cerevisiae to classical DNA-damaging agents
    Nicholas E Burgis
    Biological Engineering Division and Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA
    Chem Res Toxicol 20:1843-53. 2007
    ..These results identify the regulation of protein degradation as an important factor in the recovery of cells from toxicity induced by classical DNA-damaging agents...
  13. ncbi Protective effect of linalool, myrcene and eucalyptol against t-butyl hydroperoxide induced genotoxicity in bacteria and cultured human cells
    D Mitić-Culafić
    Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 3 II, 11000 Belgrade, Serbia
    Food Chem Toxicol 47:260-6. 2009
    ..The results indicate that linalool, eucalyptol and myrcene have substantial protective effect against oxidant induced genotoxicity, which is predominately mediated by their radical scavenging activity...
  14. ncbi Investigation of anti-oxidative, cytotoxic, DNA-damaging and DNA-protective effects of plant volatiles eugenol and borneol in human-derived HepG2, Caco-2 and VH10 cell lines
    Darina Slamenova
    Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia
    Mutat Res 677:46-52. 2009
    ..These results do not indicate any correlation between the DNA-protective and the anti-oxidative capacities of eugenol and borneol...
  15. pmc The CHROMEVALOA database: a resource for the evaluation of Okadaic Acid contamination in the marine environment based on the chromatin-associated transcriptome of the mussel Mytilus galloprovincialis
    Victoria Suárez-Ulloa
    Chromatin Structure and Evolution Group CHROMEVOL XENOMAR, Department of Cellular and Molecular Biology, University of A Coruna, E15071 A Coruña, Spain
    Mar Drugs 11:830-41. 2013
    ....
  16. pmc ENU-induced phenovariance in mice: inferences from 587 mutations
    Carrie N Arnold
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    BMC Res Notes 5:577. 2012
    ....
  17. ncbi A single amino acid governs enhanced activity of DinB DNA polymerases on damaged templates
    Daniel F Jarosz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 439:225-8. 2006
    ....
  18. ncbi Mutagens interfere with microRNA maturation by inhibiting DICER. An in silico biology analysis
    Matteo Ligorio
    Department of Health Sciences, University of Genoa, Genoa, Italy
    Mutat Res 717:116-28. 2011
    Exposure to environmental mutagens results in alteration of microRNA expression mainly oriented towards down-regulation, as typically observed in cigarette smoke. However, the molecular mechanism triggering this event is still unknown...
  19. ncbi Genotoxic effects of silver nanoparticles stimulated by oxidative stress in human normal bronchial epithelial (BEAS-2B) cells
    Ha Ryong Kim
    School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi Do, South Korea
    Mutat Res 726:129-35. 2011
    ..These results suggest that Ag-NPs have genotoxic effects in BEAS-2B cells and that oxidative stress stimulated by Ag-NPs may be an important factor in their genotoxic effects...
  20. ncbi Genetic toxicity assessment: employing the best science for human safety evaluation part III: the comet assay as an alternative to in vitro clastogenicity tests for early drug candidate selection
    Irene Witte
    Institute for Biology and Environmental Sciences, University of Oldenburg, Germany
    Toxicol Sci 97:21-6. 2007
    ..Therefore, the in vitro comet assay is proposed as an alternative to cytogenetic assays in early genotoxicity/photogenotoxicity screening of drug candidates...
  21. ncbi Ochratoxin a causes DNA damage and cytogenetic effects but no DNA adducts in rats
    Angela Mally
    Department of Toxicology, University of Wurzburg, Germany
    Chem Res Toxicol 18:1253-61. 2005
    ....
  22. ncbi Genome-wide, large-scale production of mutant mice by ENU mutagenesis
    M H Hrabe de Angelis
    Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany
    Nat Genet 25:444-7. 2000
    ..Our mutant lines are freely accessible to non-commercial users (for information, see http://www.gsf.de/ieg/groups/enu-mouse.html)...
  23. ncbi Effect of 6-gingerol on pro-inflammatory cytokine production and costimulatory molecule expression in murine peritoneal macrophages
    Sudipta Tripathi
    Department of Surgery, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Surg Res 138:209-13. 2007
    ....
  24. pmc Gamma radiation increases endonuclease-dependent L1 retrotransposition in a cultured cell assay
    Evan A Farkash
    Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Nucleic Acids Res 34:1196-204. 2006
    ..The mobilization of L1 in irradiated cells potentially contributes to genomic instability and could be a driving force for secondary mutations in patients undergoing radiation therapy...
  25. ncbi Evaluation of the clastogenic, DNA intercalative, and topoisomerase II-interactive properties of bioflavonoids in Chinese hamster V79 cells
    Ronald D Snyder
    Bristol Myers Squibb Pharma Company, Newark, DE, USA
    Environ Mol Mutagen 40:266-76. 2002
    ..These studies further validate the use of cell-based approaches for detecting drug/topo II interactions and raise interesting questions relating to biological and chemical mechanisms of flavonoids...
  26. pmc Role of the Saccharomyces cerevisiae Rad51 paralogs in sister chromatid recombination
    Amy M Mozlin
    Department of Microbiology, Columbia University Medical Center, New York, New York 10032, USA
    Genetics 178:113-26. 2008
    ..Furthermore, these data suggest that most spontaneous SCR initiates from single-stranded gaps formed at stalled replication forks rather than DSBs...
  27. ncbi DNA damaging potential of zinc oxide nanoparticles in human epidermal cells
    Vyom Sharma
    Developmental Toxicology Division, Indian Institute of Toxicology Research, Formerly Industrial Toxicology Research Centre, Council of Scientific and Industrial Research CSIR, Mahatma Gandhi Marg, P O Box 80, Lucknow 226001, Uttar Pradesh, India
    Toxicol Lett 185:211-8. 2009
    ..Hence, caution should be taken in their use in dermatological preparations as well as while handling...
  28. pmc Dynamic changes in protein-protein interaction and protein phosphorylation probed with amine-reactive isotope tag
    Marcus B Smolka
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093, USA
    Mol Cell Proteomics 4:1358-69. 2005
    ....
  29. ncbi The Ames Salmonella/microsome mutagenicity assay
    K Mortelmans
    Molecular and Genetic Toxicology Program, SRI International, Menlo Park, CA 94025 3493, USA
    Mutat Res 455:29-60. 2000
    ..These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms...
  30. ncbi ENU mutagenesis in the mouse: application to human genetic disease
    Patrick M Nolan
    Neurobehavioural Genetics Group, Medical Research Council Mammalian Genetics Unit, Harwell, Didcot, UK
    Brief Funct Genomic Proteomic 1:278-89. 2002
    ..of information that has emerged from human and mouse genome sequencing projects, the use of high-efficiency point mutagens such as N-ethyl-N-nitrosourea (ENU) and the application of systematic hierarchical screening protocols for the ..
  31. ncbi Genotoxicity of urethane dimethacrylate, a tooth restoration component
    Tomasz Poplawski
    Department of Molecular Genetics, University of Lodz, Banacha 12 16, 90 237 Lodz, Poland
    Toxicol In Vitro 24:854-62. 2010
    ..Vitamin C and chitosan decreased genotoxic effect of UDMA. Our results show that monomers of UDMA may exert pronounced cyto- and genotoxic effects in human lymphocytes and chitosan can be considered as a protection against such effects...
  32. pmc Effect of processed and red meat on endogenous nitrosation and DNA damage
    Annemiek M C P Joosen
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Carcinogenesis 30:1402-7. 2009
    ..05), which needs to be clarified in further studies. Meats cured with nitrite have the same effect as fresh RM on endogenous nitrosation but show increased FW-induced oxidative DNA damage...
  33. ncbi Evidence that hydrogen sulfide is a genotoxic agent
    Matias S Attene-Ramos
    Department of Animal Sciences, University of Illinois, Urbana, IL, USA
    Mol Cancer Res 4:9-14. 2006
    ..These data indicate that given a predisposing genetic background that compromises DNA repair, H2S may lead to genomic instability or the cumulative mutations found in adenomatous polyps leading to colorectal cancer...
  34. pmc Formaldehyde and leukemia: epidemiology, potential mechanisms, and implications for risk assessment
    Luoping Zhang
    School of Public Health, University of California, Berkeley, California 94720 7356, USA
    Environ Mol Mutagen 51:181-91. 2010
    ....
  35. pmc TOR signaling is a determinant of cell survival in response to DNA damage
    Changxian Shen
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    Mol Cell Biol 27:7007-17. 2007
    ..Thus, TOR-dependent cell survival in response to DNA-damaging agents coincides with increased mutation rates, which may contribute to the acquisition of chemotherapeutic drug resistance...
  36. ncbi Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells
    Günter Speit
    Universitat Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 613:1-9. 2006
    ..On the basis of the available data, it is not yet possible to assess the local genotoxicity of FA in humans and to draw meaningful conclusions with regard to a dose-effect relationship for risk estimation...
  37. ncbi Mutagenicity testing for chemical risk assessment: update of the WHO/IPCS Harmonized Scheme
    David A Eastmond
    University of California, Riverside, USA
    Mutagenesis 24:341-9. 2009
    ..The approach presented in this paper focuses on the identification of mutagens and genotoxic carcinogens...
  38. ncbi Non-linear dose-response of DNA-reactive genotoxins: recommendations for data analysis
    George E Johnson
    DNA Damage Group, School of Medicine, Swansea University, Wales, UK
    Mutat Res 678:95-100. 2009
    ..In this report, we will discuss the approaches that were taken to investigate potential threshold dose-response curves for DNA-reactive genotoxic compounds, with recommendations for further studies...
  39. ncbi Genetic and environmental factors in cancer and neurodegenerative diseases
    Lucia Migliore
    Dipartimento di Scienze dell Uomo e dell Ambiente, University of Pisa, Via S Giuseppe 22, 56126, Pisa, Italy
    Mutat Res 512:135-53. 2002
    ....
  40. pmc Whole-genome profiling of mutagenesis in Caenorhabditis elegans
    Stephane Flibotte
    Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    Genetics 185:431-41. 2010
    ..We describe the spectrum of mutations induced by three commonly used mutagens: ethyl methanesulfonate (EMS), N-ethyl-N-nitrosourea (ENU), and ultraviolet trimethylpsoralen (UV/TMP) in the ..
  41. ncbi Genetic susceptibility to lung cancer: the role of DNA damage and repair
    Margaret R Spitz
    Department of Epidemiology, The University of Texas, M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Epidemiol Biomarkers Prev 12:689-98. 2003
  42. ncbi The comet assay: mechanisms and technical considerations
    M Klaude
    Department of Radiobiology, Stockholm University, Sweden
    Mutat Res 363:89-96. 1996
    ..Further, the effects of alkaline concentration and sodium chloride during unwinding and electrophoresis are discussed. Throughout the study, a protocol for drying and fixation of the comets has been used...
  43. ncbi Genotoxicity and potential carcinogenicity of cyanobacterial toxins - a review
    Bojana Zegura
    National Institute of Biology, Department for Genetic Toxicology and Cancer Biology, Vecna pot 111, 1000 Ljubljana, Slovenia
    Mutat Res 727:16-41. 2011
    ..Future research efforts should focus on the elucidation of the carcinogenic potential of NOD, CYN, and the mixture of cyanobacterial extracts, as well as on the identification of possible novel toxins...
  44. ncbi Arsenicals affect base excision repair by several mechanisms
    Franziska Ebert
    Institute of Food Chemistry, University of Munster, Corrensstrasse 45, 48149 Munster, Germany
    Mutat Res 715:32-41. 2011
    ....
  45. pmc Mutational pattern and frequency of induced nucleotide changes in mouse ENU mutagenesis
    K Ryo Takahasi
    Population and Quantitative Genomics Team, RIKEN Genomic Sciences Center, Yokohama, Japan
    BMC Mol Biol 8:52. 2007
    ....
  46. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  47. pmc Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast
    Denise Hanway
    Department of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:10605-10. 2002
    ..Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function...
  48. ncbi N-methylpurine DNA glycosylase plays a pivotal role in the threshold response of ethyl methanesulfonate-induced chromosome damage
    Zoulikha M Zaïr
    Institute of Life Science, Swansea University, Swansea, SA2 8PP, UK
    Toxicol Sci 119:346-58. 2011
    ....
  49. ncbi Insensitivity of the in vitro cytokinesis-block micronucleus assay with human lymphocytes for the detection of DNA damage present at the start of the cell culture
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 27:743-7. 2012
    ..The main difference between these two applications is the way lymphocytes are exposed to mutagens. In order to maximise the probability of detecting a mutagen, the OECD guideline 487 recommends starting the ..
  50. ncbi Dynamic changes of XPA, XPC, XPF, XPG and ERCC1 protein expression and their correlations with levels of DNA damage in human bronchial epithelia cells exposed to benzo[a]pyrene
    Jin Yang
    Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, 430030 Wuhan, China
    Toxicol Lett 174:10-7. 2007
    ..Our results suggested ERCC1 may be an important limiting factor for NER, but the mechanisms underlying this observation needs further investigation...
  51. ncbi Effect-directed analysis of mutagens and ethoxyresorufin-o-deethylase inducers in aquatic sediments
    Werner Brack
    Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research, Permoserstrasse 15, D 04318 Leipzig, Germany
    Environ Toxicol Chem 24:2445-58. 2005
    ..This suggests that hazard and risk assessment of complex environmental mixtures should make increasing attempts to identify and consider hazardous key pollutants rather than focusing on a priori-selected key pollutants alone...
  52. ncbi Evaluation of chemicals requiring metabolic activation in the EpiDerm™ 3D human reconstructed skin micronucleus (RSMN) assay
    Marilyn J Aardema
    Marilyn Aardema Consulting LLC, Fairfield, OH 45014, USA
    Mutat Res 750:40-9. 2013
    ..At this point in time, for general testing, the use of a longer treatment period in situations where the standard 48h treatment is negative or questionable is recommended...
  53. ncbi Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay
    Hifzur R Siddique
    Embryotoxicology Section, Industrial Toxicology Research Center, PO Box 80, M G Marg, Lucknow, 226001, Uttar Pradesh, India
    Mutagenesis 20:285-90. 2005
    ..The present study validated our previous observation and shows that D.melanogaster is a sensitive and suitable model for the in vivo assessment of genotoxicity using our modified alkaline Comet assay...
  54. ncbi Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: a follow-up to an ECVAM workshop
    David Kirkland
    Covance Laboratories Ltd, Otley Road, Harrogate HG3 1PY United Kingdom
    Mutat Res 653:99-108. 2008
    ....
  55. ncbi Do dose response thresholds exist for genotoxic alkylating agents?
    G J S Jenkins
    Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP and School of Biological Sciences, University of Wales, Swansea, UK
    Mutagenesis 20:389-98. 2005
    ..Finally, genotoxic thresholds are currently being described for acute exposures to single agents in vitro, however, dose response data for chronic exposures to complex mixtures are, as yet, a long way off...
  56. ncbi DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco. Cellular and acellular Comet assays
    Tomas Gichner
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, 16000 6, Prague, Czech Republic
    Mutat Res 535:187-93. 2003
    ..Differences in both systems may reveal the participation of scavengers and of repair and metabolic enzymes on the activity of the genotoxic agent and the role of the cell wall in preventing the agent from reacting with nuclear DNA...
  57. ncbi Lethal mutagenesis of viruses
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, 28049, Madrid, Spain
    Curr Opin Virol 1:419-22. 2011
    ..It is derived from the error threshold relationship of quasispecies theory, and it is slowly finding its way towards a clinical application. We summarize the current situation of research in this field of antiviral therapy...
  58. ncbi Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide
    E Horvathova
    Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava, Slovakia
    Food Chem Toxicol 47:1318-23. 2009
    ..14 or 34.28 mg/kg it reduces genotoxicity of H2O2 in both hepatocytes and testicular cells...
  59. pmc Genotoxicity of Escherichia coli Nissle 1917 strain cannot be dissociated from its probiotic activity
    Maiwenn Olier
    Neuro gastroenterologie et Nutrition, UMR INRA ENVT 1331, Toulouse, France
    Gut Microbes 3:501-9. 2012
    ..Our data provide additional evidence for the benefit of this currently used probiotic in colitis but remind us that an efficient probiotic may also have side effects as any other medication...
  60. pmc Genotoxic stress causes the accumulation of the splicing regulator Sam68 in nuclear foci of transcriptionally active chromatin
    Roberta Busà
    Department of Public Health and Cell Biology, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy
    Nucleic Acids Res 38:3005-18. 2010
    ..These results strongly suggest that Sam68 is part of a RNA-mediated stress response of the cell that modulates alternative splicing in response to DNA damage...
  61. ncbi Intralaboratory and interlaboratory evaluation of the EpiDerm 3D human reconstructed skin micronucleus (RSMN) assay
    Ting Hu
    The Procter and Gamble Co, P O Box 538707, Cincinnati, OH 45253, United States
    Mutat Res 673:100-8. 2009
    ..These results indicate the RSMN assay using the EpiDerm 3D human skin model is a promising new in vitro genotoxicity assay that allows evaluation of chromosome damage following "in vivo-like" dermal exposures...
  62. ncbi Comet-FISH with rDNA probes for the analysis of mutagen-induced DNA damage in plant cells
    Jolanta Kwaśniewska
    Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40 032 Katowice, Poland
    Environ Mol Mutagen 53:369-75. 2012
    ..Moreover, our work suggests that comet-FISH in plants may be a useful tool for environmental monitoring assessment...
  63. pmc Effect of chemical mutagens and carcinogens on gene expression profiles in human TK6 cells
    Lode Godderis
    Occupational, Environmental and Insurance Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    PLoS ONE 7:e39205. 2012
    ..Our findings expand our understanding of the transcriptomic response to genotoxic carcinogens, revealing the alteration of diverse sets of genes and pathways involved in cellular homeostasis and cell cycle control...
  64. ncbi Further development of the EpiDerm 3D reconstructed human skin micronucleus (RSMN) assay
    Greg C Mun
    Institute for In Vitro Sciences Inc, 30 W Watkins Mill Road, Suite 100, Gaithersburg, MD 20878, USA
    Mutat Res 673:92-9. 2009
    ....
  65. pmc Nutrient-sensitive mitochondrial NAD+ levels dictate cell survival
    Hongying Yang
    Department of Pathology, Paul F Glenn Laboratories, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 130:1095-107. 2007
    ..We discuss the relevance of these findings to understanding how nutrition modulates physiology and to the evolution of apoptosis...
  66. ncbi A review of the mutagenicity and rodent carcinogenicity of ambient air
    Larry D Claxton
    Environmental Carcinogenesis Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Mutat Res 636:36-94. 2007
    ..Air sheds contain similar types and classes of mutagens; however, the levels of these compounds vary considerably among air sheds...
  67. ncbi Carbon nanotubes induce oxidative DNA damage in RAW 264.7 cells
    Lucia Migliore
    Department of Human and Environmental Sciences, Faculty of Medicine, University of Pisa, Via S Giuseppe 22, 56100 Pisa, Italy
    Environ Mol Mutagen 51:294-303. 2010
    ..7 cells. Moreover, the modified version of the comet assay allows the specific detection of the induction of oxidative damage to DNA, which may be the underlying mechanism involved in the CNT-associated genotoxicity...
  68. ncbi Development of a method for assessing micronucleus induction in a 3D human skin model (EpiDerm)
    Rodger D Curren
    Institute for In Vitro Sciences Inc, 21 Firstfield Road, Suite 220, Gaithersburg, MD 20878, USA
    Mutat Res 607:192-204. 2006
    ..It is hoped that other investigators utilize these methods to further the understanding of this potentially valuable new non-animal method...
  69. pmc Three prime exonuclease I (TREX1) is Fos/AP-1 regulated by genotoxic stress and protects against ultraviolet light and benzo(a)pyrene-induced DNA damage
    Markus Christmann
    Department of Toxicology, University Medical Center, Obere Zahlbacher Strasse 67, D 55131 Mainz, Germany
    Nucleic Acids Res 38:6418-32. 2010
    ..The data revealed trex1 as a novel DNA damage-inducible repair gene that plays a protective role in the genotoxic stress response...
  70. pmc The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer
    Peter E M Gibbs
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Genetics 169:575-82. 2005
    ..In contrast, bypass of the T-T dimer in the pol32delta strain occurs at the wild-type frequency...
  71. ncbi Effects of surface chemistry on cytotoxicity, genotoxicity, and the generation of reactive oxygen species induced by ZnO nanoparticles
    Hong Yin
    CSIRO Materials Science and Engineering, CSIRO Future Manufacturing Flagship, Clayton VIC 3168, Australia
    Langmuir 26:15399-408. 2010
    ..The lowest toxicity was achieved with a surface coating of components from a cell culture medium...
  72. ncbi Genotoxicity of vegetable cooking oils in the Drosophila wing spot test
    Mar Rojas-Molina
    Department of Genetics, University of Cordoba, Cordoba, Spain
    Environ Mol Mutagen 45:90-5. 2005
    ..It is argued that the genotoxicity is probably due to the fatty acid composition of the oils, which after peroxidation can form specific DNA-adducts...
  73. ncbi Cancer, cadmium and genome integrity
    Cynthia T McMurray
    Nat Genet 34:239-41. 2003
  74. ncbi Neurosprora crassa RAD5 homologue, mus-41, inactivation results in higher sensitivity to mutagens but has little effect on PCNA-ubiquitylation in response to UV-irradiation
    Tsuyoshi Kawabata
    Department of Regulation Biology, Saitama University, Sakura ku Shimo ookubo 255, Saitama City, Saitama 338 8570, Japan
    Curr Genet 52:125-35. 2007
    ..crassa. However, mus-41 is not essential for the ubiquitylation of PCNA that is detected in the wild-type background, suggesting that there is another ubiquitin ligase catalyzing ubiquitylation of PCNA in response to UV in N. crassa...
  75. ncbi Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624
    Jianqiao Wang
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan
    FEMS Microbiol Lett 314:164-9. 2011
    ..This is the first report that MnP can effectively remove the mutagenic activity of AFB(1) by converting it into AFB(1) -8,9-dihydrodiol...
  76. ncbi The reconstructed skin micronucleus assay (RSMN) in EpiDerm™: detailed protocol and harmonized scoring atlas
    Erica L Dahl
    Institute for In Vitro Sciences, Inc, Gaithersburg, MD, USA
    Mutat Res 720:42-52. 2011
    ..We also describe factors that can influence the performance of the assay. Use of these methods will help new laboratories to conduct the assay, thereby further increasing the database for this promising new in vitro genotoxicity test...
  77. ncbi Allium cepa test in environmental monitoring: a review on its application
    Daniela Morais Leme
    Departamento de Biologia, Instituto de Biociencias, Universidade Estadual Paulista UNESP, Rio Claro, SP, Brazil
    Mutat Res 682:71-81. 2009
    Higher plants are recognized as excellent genetic models to detect environmental mutagens and are frequently used in monitoring studies...
  78. ncbi Mutagenesis of benzo[a]pyrene diol epoxide in yeast: requirement for DNA polymerase zeta and involvement of DNA polymerase eta
    Zhongwen Xie
    Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, USA
    Biochemistry 42:11253-62. 2003
    ....
  79. pmc Comparison of p53 mutations induced by PAH o-quinones with those caused by anti-benzo[a]pyrene diol epoxide in vitro: role of reactive oxygen and biological selection
    Yu Min Shen
    Department of Pharmacology, Center of Excellence in Environmental Toxicology and Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    Chem Res Toxicol 19:1441-50. 2006
    ..The resultant 8-oxo-dGuo yields a pattern of mutations but not a spectrum consistent with that seen in lung cancer; we suggest that the emergence of the spectrum requires biological selection...
  80. ncbi N-ethyl-N-nitrosourea (ENU) increased brain mutations in prenatal and neonatal mice but not in the adults
    William Slikker
    College of Letters and Science, University of California, Los Angeles 90024, USA
    Toxicol Sci 81:112-20. 2004
    ..These results demonstrate a differential mutagenic effect of ENU on the mouse brain depending on the stages of development and suggest an enhanced susceptibility of brain cancer hazard for perinatal exposure to genotoxicants...
  81. pmc Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis
    Elizabeth A Greene
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 164:731-40. 2003
    ..As expected for alkylation of guanine by EMS, >99% of mutations are G/C-to-A/T transitions. A nearest-neighbor bias around the mutated base pair suggests that mismatch repair counteracts alkylation damage...
  82. ncbi Hijacking of the human alkyl-N-purine-DNA glycosylase by 3,N4-ethenocytosine, a lipid peroxidation-induced DNA adduct
    Laurent Gros
    Groupe Réparation de l ADN, CNRS Unité Mixte de Recherche 8113 LBPA ENS Cachan, Institut Gustave Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex, France
    J Biol Chem 279:17723-30. 2004
    ..The proposed model of ANPG-mediated genotoxicity of epsilonC provides a new insight in the molecular basis of lipid peroxidation-induced cell death and genome instability in cancer...
  83. ncbi Possible mechanisms explaining the tendency towards interstitial fibrosis in aristolochic acid-induced acute tubular necrosis
    Li Yang
    Renal Division, Department of Medicine, First Hospital, and Institute of Nephrology, Peking University, Beijing 100034, P R China
    Nephrol Dial Transplant 22:445-56. 2007
    ..We explored possible mechanisms responsible for the inability of plerosis and the tendency towards fibrosis in aristolochic acid-induced acute tubular necrosis (AA-ATN)...
  84. ncbi Evaluation of mutagenic activities of leachates in landfill sites by micronucleus test and comet assay using goldfish
    Yuya Deguchi
    Graduate School of Nutritional and Environmental Sciences and COE Program in the 21st Century, University of Shizuoka, 52 1 Yada, Shizuoka 422 8526, Japan
    Mutat Res 627:178-85. 2007
    To develop a simple system for monitoring the presence of mutagens/carcinogens in the leachates from landfill sites, we used a micronucleus test and a single cell gel electrophoresis (comet) assay originally developed for mice and rats on ..
  85. ncbi Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene despite robust mRNA transcriptional response
    Carole Lyn Yauk
    Environmental Health Sciences and Research Bureau, Health Canada, ON, Canada
    Mutat Res 722:131-9. 2011
    ..Our results are consistent with the lack of response of miRNA in rodent liver to dioxin, another potent AHR-agonist. We conclude that hepatic miRNA in vivo is not directly responsive to BaP exposure...
  86. ncbi Confounding experimental considerations in nanogenotoxicology
    S H Doak
    Institute of Life Science, Swansea University, Wales, UK
    Mutagenesis 24:285-93. 2009
    ..Thus, highlighting some of the potential confounding factors that need to be considered in order to ensure that in vitro genotoxicity assays report true biological impacts in response to nanomaterial exposure...
  87. pmc Defining EMS and ENU dose-response relationships using the Pig-a mutation assay in rats
    Krista L Dobo
    Pfizer Global Research and Development, Genetic Toxicology Center of Emphasis, Eastern Point Road, Groton, CT 06340, United States
    Mutat Res 725:13-21. 2011
    ..has accumulated that supports the existence of sublinear dose-response relationships at low doses of DNA reactive mutagens. However, creating the in vivo data necessary to allow for a more detailed dose-response modeling with the ..
  88. ncbi Gender-related differences in response to mutagens and carcinogens
    M Kirsch-Volders
    Laboratory for Cell Genetics, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Mutagenesis 25:213-21. 2010
    ..This commentary focuses on several aspects of gender-related differences in responses to mutagens and carcinogens, including sensitivity to chromosome damage, the contribution of genotypic variation and the role ..
  89. pmc Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage
    A Klungland
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 96:13300-5. 1999
    ....
  90. pmc A rich TILLING resource for studying gene function in Brassica rapa
    Pauline Stephenson
    Department of Crop Genetics, John Innes Centre, Norwich, NR4 7UH, UK
    BMC Plant Biol 10:62. 2010
    ..rapa. Moreover, the ongoing sequencing of the B. rapa genome makes this species a highly attractive model for Brassica research and genetic resource development...
  91. ncbi Effect of the dibenzylbutyrolactone lignan (-)-hinokinin on doxorubicin and methyl methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts
    Flávia Aparecida Resende
    Faculdade de Ciencias Farmaceuticas de Araraquara, Universidade Estadual Paulista Julio de Mesquita Filho, Rodovia Araraquara Jaú Km 1, 14801 902 Araraquara, Sao Paulo, Brazil
    Mutat Res 700:62-6. 2010
    ..In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity...
  92. ncbi Monitoring homologous recombination following replication fork perturbation in the fission yeast Schizosaccharomyces pombe
    Fekret Osman
    Department of Biochemistry, University of Oxford, Oxford, UK
    Methods Mol Biol 521:535-52. 2009
    ..The strains are available on request, and here we describe in detail how some of them can be used to determine the effect of your mutation of choice on spontaneous, DNA damage-induced, and replication block-induced recombinant formation...
  93. ncbi Synergistic genotoxicity caused by low concentration of titanium dioxide nanoparticles and p,p'-DDT in human hepatocytes
    Yun Shi
    MOE Key Laboratory of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
    Environ Mol Mutagen 51:192-204. 2010
    ..Dose-dependent synergistic genotoxicity induced by combined exposure of trace p,p'-DDT and nano-TiO(2) suggests a potential environmental risk of nano-TiO(2) assisted photocatalysis...
  94. ncbi In vivo genotoxicity of EMS: statistical assessment of the dose response curves
    Elmar Gocke
    F Hoffmann La Roche Ltd, Nonclinical Safety, Basel, Switzerland
    Toxicol Lett 190:298-302. 2009
    ..While presently this conclusion cannot be generalized to other genotoxins a change of paradigm appears to be indicated at least for alkylating agents inducing a comparable type and spectrum of DNA lesions as EMS...
  95. pmc Miniaturized flow cytometric in vitro micronucleus assay represents an efficient tool for comprehensively characterizing genotoxicity dose-response relationships
    Steven M Bryce
    Litron Laboratories, Rochester, NY 14623, United States
    Mutat Res 703:191-9. 2010
    ....
  96. ncbi Heterocyclic amines: Mutagens/carcinogens produced during cooking of meat and fish
    Takashi Sugimura
    National Cancer Center, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 95:290-9. 2004
    ..in ordinary life is low and not sufficient in itself to explain human cancer, the coexistence of many other mutagens/carcinogens of either autobiotic or xenobiotic type and the possibility that HCAs induce genomic instability and ..
  97. pmc Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune
    Arend F van Peer
    Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Appl Environ Microbiol 75:1243-7. 2009
    ....
  98. ncbi Nongenotoxic activation of the p53 pathway as a therapeutic strategy for multiple myeloma
    Thorsten Stühmer
    Department of Internal Medicine II, Division of Hematology and Oncology, University Clinics Würzburg, Germany
    Blood 106:3609-17. 2005
    ..Therefore, MDM2 antagonists may offer a new treatment option for this disease...
  99. ncbi Detailed review of transgenic rodent mutation assays
    Iain B Lambert
    Mutagenesis Section, Environmental Health Sciences Bureau, Healthy Environments and Consumer Safety Branch, 0803A, Health Canada, Ottawa, Ont, Canada K1A 0L2
    Mutat Res 590:1-280. 2005
    ..5) Recommendations are made regarding the experimental parameters for TGR assays, and the use of TGR assays in a regulatory context...
  100. ncbi Methylated trivalent arsenicals as candidate ultimate genotoxic forms of arsenic: induction of chromosomal mutations but not gene mutations
    Andrew D Kligerman
    Environmental Carcinogenesis Division, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Environ Mol Mutagen 42:192-205. 2003
    ..and DMA(III) were the most potent clastogens of the six arsenicals in human lymphocytes and the most potent mutagens of the six arsenicals at the Tk(+/-) locus in mouse lymphoma cells...
  101. ncbi Genetic susceptibility of newborn daughters to oxidative stress
    Ilse Decordier
    Laboratory of Cell Genetics, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
    Toxicol Lett 172:68-84. 2007
    A central question in risk assessment is whether newborns' susceptibility to mutagens is different from that of adults...

Research Grants63

  1. DNA Damage Checkpoint and Cancer
    Toru Ouchi; Fiscal Year: 2012
    ..processes such as metabolic reactions and DNA replication, and through exposure of cells to environmental mutagens including chemical carcinogens, UV light and gamma-radiation...
  2. PROBES FOR CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 2013
    ..to measure DDR in response to different types of DNA damage will be assessed including that caused by drugs, mutagens and radiation, and correlated with cell cycle phase and induction of apoptosis...
  3. Determinants of Coronavirus Fidelity in Replication and Pathogenesis
    Mark R Denison; Fiscal Year: 2013
    ..CoV ExoN- mutator viruses manifest decreased fitness compared to ExoN+ (WT) viruses, are sensitive to RNA mutagens, and are stably attenuated in mouse models of lethal SARS-CoV infection...
  4. Regulation of Nucleotide Excision Repair by Proteolysis
    Pengbo Zhou; Fiscal Year: 2013
    ..NER) is the major DNA repair machinery that removes DNA damage induced by ultraviolet light (UV) and chemical mutagens to prevent genomic instability and tumorigenesis...
  5. A new synergy for flavivirus therapy: RNAi enhancement and viral mutagens
    KATHRYN ALYCE HANLEY; Fiscal Year: 2012
    ..If this hypothesis is correct, the finding of this study will represent a significant advance in the development of new therapies for flaviviral disease. ..
  6. Polymorphic variants of human DNA glycosylase NEIL2 and lung cancer susceptibilit
    Tapas K Hazra; Fiscal Year: 2010
    ..cancer but will also provide definitive insights for assessing human risks associated with exposure to various mutagens in the environment...
  7. Metal inhibition of the base excision repair enzymes
    Anton Guliaev; Fiscal Year: 2013
    ..Metal toxicity represents a novel mechanism, by which various environmental mutagens and carcinogens can destabilize DNA integrity leading to mutagenic and carcinogenic events in the cell...
  8. Optimizing Wolbachia manipulation in mosquito cell lines
    Ann M Fallon; Fiscal Year: 2013
    ..We further hypothesize that treating Wolbachia-infected mosquito cells with chemical mutagens, specifically during the period when Wolbachia is engaged in active DNA synthesis, will enable recovery of ..
  9. Dissecting the role of mitochondrial DNA mutations in cancer
    Sean David Taylor; Fiscal Year: 2013
    ..e. diet and exposure to environmental mutagens) can be more efficiently developed.
  10. Dynamics of heterochromatin DNA repair: novel role of nuclear architecture
    IRENE E CHIOLO; Fiscal Year: 2013
    ..These studies will uncover the mechanisms that normal cells use to protect repeats from environmental mutagens. In addition, this research will contribute to our understanding of the mechanisms that generate chromosome ..
  11. Excision of Carcinogen-DNA Adducts in Nucleosomes
    Nicholas E Geacintov; Fiscal Year: 2013
    ..The structural features and biological impact of PAH that distinguish highly active mutagens and tumorigens from structurally related less active, or inactive compounds, have long been of interest for ..
  12. Mechanisms of Telomeric DNA Loss and Repair
    PATRICIA LYNN OPRESKO; Fiscal Year: 2010
    ..The broad goals of this proposal are to determine the impact of DNA damage and environmental mutagens on the integrity of telomeric DNA in chromosome ends, and to examine cellular pathways for repairing telomeres...
  13. Antagonism of the Ah Receptor in Controlling Breast Cancer Growth and Invasion
    Jennifer J Schlezinger; Fiscal Year: 2010
    ..also plays an important role in malignant epithelial cell growth and invasion apart from its role in formation of mutagens and in the absence of environmental chemicals...
  14. FASEB SRC on Genetic Recombination and Genome Rearrangements
    JOHN HJ PETRINI; Fiscal Year: 2011
    ..of proteins defective in these human cancer prone syndromes in genome stability and resistance to environmental mutagens. To inform and augment studies from mammals, work concerning related proteins and mechanisms from model systems ..
  15. New determinants of the DNA damage response in the fission yeast S. pombe
    MATTHEW J O'CONNELL; Fiscal Year: 2013
    ..Checkpoints are also crucial in the biology of cellular ageing and senescence, and in the response environmental mutagens. Thus, a detailed understanding of their biology is central to human disease...
  16. Travel Awards for the 11th International Conference on Environmental Mutagens
    THOMAS EDWARD WILSON; Fiscal Year: 2013
    Travel Awards for the 11th International Conference on Environmental Mutagens Abstract The purpose of this application is to request funding to support travel awards for a select number of students and invited speakers to attend the 11th ..
  17. Molecular mechanisms regulating motility and migration of muscle satellite cells
    DAWN D CORNELISON; Fiscal Year: 2010
    ..Conditions can be altered by addition of exogenous stimuli such as potential mutagens, blocking of soluble or cell-surface proteins with antibodies or peptide mimetics, addition of pharmacological ..
  18. Chk1 signaling in the G2 DNA damage checkpoint
    MATTHEW O CONNELL; Fiscal Year: 2009
    ..Checkpoints are also central to the biology of aging, response to environmental mutagens, and in developmental signals essential for tissue homeostasis...
  19. Mapping and Functional Analysis of RNA:DNA Hybrid-Forming Loci
    John Greally; Fiscal Year: 2013
    ..mapping assay, including some orthogonal approaches using other nucleases, affinity reagents and chemical mutagens. We propose to get much more detailed mass spectroscopy data from different cell types from human and mouse, and ..
  20. Y-family DNA polymerases and cellular responses to benzo[a]pyrene
    ALDEN C KLARER; Fiscal Year: 2013
    ..been done with ultraviolet radiation, and the role of these enzymes in mutagenesis and carcinogenesis by chemical mutagens remains largely unexplored...
  21. NUCKS, a novel double-strand break repair gene, implicated in cancer biology
    Claudia Wiese; Fiscal Year: 2013
    ..during DNA replication, but also can be introduced by exposure to ionizing radiation (IR) or to chemical mutagens. DSBs can lead to cell death, mutations and cancer, and defects in DSB repair (DSBR) underlie many human diseases,..
  22. Role of p300/CBP and hHR23 Proteins in p53 Regulation
    Steven R Grossman; Fiscal Year: 2013
    ..DESCRIPTION (provided by applicant):The p53 tumor suppressor counters a wide range of stresses, including chemical mutagens, ionizing radiation, hypoxia, and nutrient deprivation, by activating growth arrest or apoptosis programs...
  23. Etiology of Alkylator-Induced Myeloid Leukemia
    Michelle M Le Beau; Fiscal Year: 2012
    ..Second, the development of t-AML provides a unique opportunity to examine the effects of mutagens on carcinogenesis in humans, as well as the issue of genetic susceptibility and factors that predispose to the ..
  24. Mechanisms of Environmental and Nuclear and Mitochondrial Mutagenesis
    Jason H Bielas; Fiscal Year: 2013
    ..Thus, environmental mutagens in particular may play a substantial role in the origin and incidence of aging and disease by damaging mtDNA and ..
  25. Ultrafast Spectroscopic Methods to Probe Photodamage and Unfolding in Biopolymers
    David W McCamant; Fiscal Year: 2012
    ..Ultraviolet light is one of the most prevalent environmental mutagens on earth, with significant deleterious effects on human health...
  26. GENETIC TOXICITY IN BACTERIA AND RODENTS
    LES RECIO; Fiscal Year: 2009
    ..This exercise is being conducted to help better define the sensitivity of the CT8 assay in detecting germ cell mutagens. Keywords: Micronuclei;Ames test;salmonella;mutation;chromosomal damage
  27. GENETIC TOXICITY IN BACTERIA AND RODENTS
    LES RECIO; Fiscal Year: 2011
    ..This exercise is being conducted to help better define the sensitivity of the CT8 assay in detecting germ cell mutagens. Keywords: Micronuclei;Ames test;salmonella;mutation;chromosomal damage
  28. Mismatch Repair Functions Affected During Tumorigenesis
    Christopher D Heinen; Fiscal Year: 2012
    ..resulting from DNA polymerase errors, recombination between heterologous sequences or endogenous and exogenous mutagens. Thus, loss of MMR function results in an elevated mutation rate (mutator phenotype)...
  29. Mechanism of Mammalian Translesion DNA synthesis
    Masaaki Moriya; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cellular DNA is continuously damaged by endogenous and exogenous sources of mutagens. The long-term objectives of this proposal are (i) to understand how unrepaired damage induces mutations that ..
  30. Validation of a Rodent Mutagenicity Assay
    Stephen D Dertinger; Fiscal Year: 2012
    ..of investigation through an extensive inter-laboratory validation effort whereby rats will be treated with known mutagens and non-mutagens using both a short-term as well as a 28-day repeat dosing schedule...
  31. Biological Effects of DNA Adducts Formed by Nitroaromatic Compounds
    Zucai Suo; Fiscal Year: 2013
    ..These compounds are potent mutagens and carcinogens, and it has been suggested that some of these pollutants play a role in human lung and breast ..
  32. Environmental Mutagen Society Annual Meetings 2009 - 2013
    MICHAEL JACOB PLEWA; Fiscal Year: 2013
    ..in toxicology and disease susceptibility, and the challenges of assessing risks from environmental exposures to mutagens. Recognizing the importance of its diverse mix of professionals to exchange ideas and the latest experimental ..
  33. CELLULAR RESPONSE TO DNA ADDUCTS
    Masaaki Moriya; Fiscal Year: 2009
    Project Summary Cellular DNA is continuously damaged by endogenous and exogenous sources of mutagens. The long-term objectives of this proposal are (i) to understand how mammalian cells repair DNA damage and how unrepaired damage induces ..
  34. MOLECULAR DYNAMICS IN CELLULAR AGING: A SYNTHETIC BIOLOGY APPROACH.
    CLIFFORD LEE WANG; Fiscal Year: 2012
    ..applicant): DNA damage occurs during the course of our lives, spontaneously or through exposure to environmental mutagens. In response to DNA damage, the transcription factor p53 is activated...
  35. HEPATOBILIARY DISPOSITION IN TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2003
    ..and cyproterone acetate is dependent on the sulfotransferase-mediated bioactivation of these compounds into mutagens and carcinogens...
  36. Structure, mechanism, and inhibition of AlkB homologues
    JOHN FRANCIS HUNT; Fiscal Year: 2010
    ..on endocyclic ring nitrogen atoms, a toxic DNA modification that is mediated by both endogenous and environmental mutagens. Elucidation of the chemical reaction catalyzed by AlkB has enabled rapid progress to be made in understanding ..
  37. CELLULAR AND MOLECULAR RESPONSE TO DNA REPAIR DEFICIENCY
    Renee Reijo Pera; Fiscal Year: 2000
    ..Because of the important role XRCC1 plays in modulating the damaging effects of various mutagens, the characterization of the knockout mice and cells derived from them will improve understanding of the ..
  38. METALS/NUCLEOSIDE HYDROPEROXIDE MEDIATED GENETIC DAMAGE
    Krystyna Frenkel; Fiscal Year: 1992
    ..Many exogeneous mutagens and carcinogens act through the generation of oxygen radicals as does ionizing radiation...
  39. EFFECTS OF BENZO[A]PYRENE IN TRANSGENIC MODEL OF CERVICA
    ELLEN RORKE; Fiscal Year: 2002
    ..Metabolites of B[a]P are potent DNA mutagens. B[a]P is mutagenic in cervical cells in vitro, accumulates in cervical mucus in vivo, and ras oncogene, a known ..
  40. Direct Somatic Mutation Analysis Through Sequencing
    Jan Vijg; Fiscal Year: 2012
    ..in humans has essentially constrained a more complete understanding of individual susceptibility to environmental mutagens and their relationship to cancer, genetic disease and aging...
  41. Forward and Reverse Genetics in Chlamydia
    Raphael H Valdivia; Fiscal Year: 2013
    ..a comprehensive system for forward and reverse genetic analysis in Chlamydia based on the use of chemical mutagens, genetic recombination and "deep" DNA sequencing technologies...
  42. Lethal Mutagenesis as an Antiviral Strategy
    Craig Cameron; Fiscal Year: 2007
    ..Evaluate the ability of ribonucleotide reductase to utilize mutagens as substrates and/or effectors in order to obtain information on potential issues of safety; [unreadable] 3...
  43. ANAEROBIC METABOLISM OF MUTAGENS IN HUMAN FOODS
    David Kingston; Fiscal Year: 1990
    ....
  44. FOLATE EFFECT ON CHROMOSOMES IN BREAST CANCER
    RICHARD BRANDA; Fiscal Year: 2000
    ..Folic acid deficiency is associated with numerous chromosomal changes and enhances the genetic damage caused by mutagens. Therefore, folic acid supplementation may be a cancer control strategy relevant to large populations to reduce ..
  45. Developing transposon methods for insertional mutagenesis in Xenopus
    Mustafa K Khokha; Fiscal Year: 2010
    ..We outline a series of experiments to develop tol2 and piggybac transposons as insertional mutagens in X. tropicalis...
  46. ZYGOTIC DNA REPAIR OF ENVIRONMENT INDUCED SPERM LESIONS
    ANDREW WYROBEK; Fiscal Year: 2000
    ..repair genes that maintain genomic integrity after fertilization with sperm from fathers exposed to environmental mutagens. It is well established that exposure of male mice to mutagens before mating can induce high frequencies of ..
  47. Quantifying the impact of diet on carcinogen exposure
    JAMES FELTON; Fiscal Year: 2003
    ..Cooked muscle meats contain potent mutagens and rodent carcinogens belonging to the heterocyclic amine (HA) class of compounds...
  48. Gamma-ray-induced mutagen sensitivity in breast cancer
    Li E Wang; Fiscal Year: 2005
    ..is a marker of cellular repair capacity for chromosomal damage induced by endogenous or exogenous mutagens. DNA double-strand break repair (DSBR), including non-homologous end joining (NHEJ) and homologous recombination (..
  49. Dietary affects upon genomic methylation in Trout cancer
    Joseph Brunelli; Fiscal Year: 2005
    ..the variability in methylation to development of nephroblastomas and hepatocarcinomas through exposure to mutagens in clonal lines of rainbow trout...
  50. DNA REPLICATION AFTER INSULT WITH UV
    THOMAS GRIFFITHS; Fiscal Year: 1991
    ..From studies with ultraviolet (UV) radiation, ionizing radiation, and other mutagens/carcinogens it is clear that DNA is the critical target for physical and chemical carcinogens and mutagens...
  51. OCCUPATIONAL EXPOSURES AND RATES OF DNA DAMAGE
    Lawrence Fine; Fiscal Year: 1980
    The objective of this study is to investigate the relationship between DNA damage and exposure to carcinogens or mutagens in selected occupations...
  52. PROTECTION FROM ENVIRONMENTAL TOXINS/MUTAGENS BY INDOLES
    HOWARD SHERTZER; Fiscal Year: 1990
    ..These studies will provide the basis for developing dietary and pharmacological strategies to reduce the risk associated with exposure to toxins that act via electrophilic or radical mechanisms...
  53. Development of a High Throughput Genotoxicity Assay
    NEAL HUEBOTTER; Fiscal Year: 2004
    ..Preliminary experiments funded by HESS, Inc. have demonstrated the proposed assay's potential for detecting mutagens more rapidly and more sensitively than previously possible with the Ames assay...
  54. TRANSGENIC FISH MODELS FOR MUTAGENESIS STUDIES
    Richard Winn; Fiscal Year: 2004
    ..dual needs for improved methods to assess genetic health risks associated with exposure to chemical and physical mutagens, and for non-mammalian models for comparative mechanistic studies of in vivo mutagenesis...
  55. ROLE OF ENVIRONMENTAL MUTAGENS IN POOR PREGNANCY OUTCOME
    Philip Iannaccone; Fiscal Year: 1990
    ..Finally we will attempt to intervene in electrophilic toxic damage to the developing embryo in transgenic mice produced to overexpress the enzyme glutathione-S-transferase (Ya) which we have produced...
  56. MOLECULAR GENETIC BASIS OF MOUSE B CELL LYMPHOMAS
    Monica Justice; Fiscal Year: 1999
    ..These transposable elements act as insertional mutagens and serve as useful molecular tags to clone new genes associated with tumorigenesis...
  57. BIOLOGICAL MARKERS IN CIS DDP TREATED TESTICULAR CANCER
    Frederica Perera; Fiscal Year: 1990
    ..Thus it is a model compound for purposes of validating methods to biomonitor humans with exposure to mutagens/carcinogens...
  58. RELATIONSHIPS OF FECAL MUTAGENS TO COLON CANCER
    Tracy Wilkins; Fiscal Year: 1992
    ..A decrease in their concentrations then could predispose a person to colonic cancer...
  59. PRIMARY DNA LESIONS AS SIGNALS FOR P53
    Jack Griffith; Fiscal Year: 1999
    ..p53 is activated by a cellular pathway that responds to DNA damage caused by X-rays, u.v. light, mutagens and oxidation, and leads to a block in the cell cycle to allow time for repair, or apoptosis...
  60. MECHANISMS OF DNA DEPENDENT CYTOTOXICITY BY CHEMICALS
    JOE GRISHAM; Fiscal Year: 1992
    ..MNNG and BPDE are potent mutagens at the Na+/K+ ATPase locus, and the induction of both cytotoxicity and mutagenesis are strongly cell cycle-..
  61. EMBRYOS FOR DETECTING ENVIRONMENTAL MUTAGENS
    Stephen Bloom; Fiscal Year: 1992
    ..In addition, this research contributes to the development of an in vivo assay for detecting mutagens and their long-range effects resulting from prenatal exposure...
  62. THE METABOLIC FATE OF MUTAGENIC AMINES IN ANIMALS & MAN
    DONALD DAVIES; Fiscal Year: 1991
    ..program of research is to conduct a thorough study of the absorption and metabolic activation in man of the major mutagens, MeIQx, DiMeIQx and PhIP, produced during the cooking of beef...
  63. QUINOLINES AND CARBAZOLES: MUTAGENS AND CARCINOGENS
    EDMOND LAVOIE; Fiscal Year: 1993
    ..9-Hydroxycarbazole and 9- acetoxycarbazole are direct-acting mutagens in S...