molecular probes

Summary

Summary: A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.

Top Publications

  1. ncbi Molecular beacons: probes that fluoresce upon hybridization
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 14:303-8. 1996
  2. pmc Quantum dots for live cells, in vivo imaging, and diagnostics
    X Michalet
    Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Science 307:538-44. 2005
  3. ncbi Visualizing the mechanical activation of Src
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, La Jolla, California 92093, USA
    Nature 434:1040-5. 2005
  4. pmc Protein scaffold-based molecular probes for cancer molecular imaging
    Zheng Miao
    Department of Radiology, Molecular Imaging Program at Stanford, Stanford University, CA 94305 5344, USA
    Amino Acids 41:1037-47. 2011
  5. ncbi Specific detection of Campylobacter jejuni using the bacteriophage NCTC 12673 receptor binding protein as a probe
    Amit Singh
    Department of Electrical and Computer Engineering and University of Alberta, Edmonton, AB, Canada
    Analyst 136:4780-6. 2011
  6. ncbi Deuteration of a molecular probe for DNP hyperpolarization--a new approach and validation for choline chloride
    Hyla Allouche-Arnon
    Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Contrast Media Mol Imaging 6:499-506. 2011
  7. ncbi Targeted molecular probes for imaging atherosclerotic lesions with magnetic resonance using antibodies that recognize oxidation-specific epitopes
    Karen C Briley-Saebo
    Imaging Science Laboratory, Department of Radiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Circulation 117:3206-15. 2008
  8. ncbi Molecular probes for P2X7 receptor studies
    Hendra Gunosewoyo
    School of Chemistry, University of Sydney, NSW 2006 Australia
    Curr Med Chem 14:1505-23. 2007
  9. ncbi Surface modification of cetyltrimethylammonium bromide-capped gold nanorods to make molecular probes
    Chenxu Yu
    Department of Agricultural and Biological Engineering, Bindley Biosciences Center, Purdue University, 225 S University Street, West Lafayette, Indiana 47907, USA
    Langmuir 23:9114-9. 2007
  10. ncbi Application of RGD-containing peptides as imaging probes for alphavbeta3 expression
    Ingrid Dijkgraaf
    Technische Universitat Munchen, Department of Nuclear Medicine, Klinikum rechts der Isar, 81675 Munchen, Germany
    Front Biosci (Landmark Ed) 14:887-99. 2009

Detail Information

Publications290 found, 100 shown here

  1. ncbi Molecular beacons: probes that fluoresce upon hybridization
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 14:303-8. 1996
    ..When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced...
  2. pmc Quantum dots for live cells, in vivo imaging, and diagnostics
    X Michalet
    Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Science 307:538-44. 2005
    ....
  3. ncbi Visualizing the mechanical activation of Src
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, La Jolla, California 92093, USA
    Nature 434:1040-5. 2005
    ..We find that the transmission of mechanically induced Src activation is a dynamic process that directs signals via the cytoskeleton to spatial destinations...
  4. pmc Protein scaffold-based molecular probes for cancer molecular imaging
    Zheng Miao
    Department of Radiology, Molecular Imaging Program at Stanford, Stanford University, CA 94305 5344, USA
    Amino Acids 41:1037-47. 2011
    ..For the purpose of development of new generation molecular probes, various protein scaffold molecules have been labeled with imaging moieties and evaluated both in vitro and ..
  5. ncbi Specific detection of Campylobacter jejuni using the bacteriophage NCTC 12673 receptor binding protein as a probe
    Amit Singh
    Department of Electrical and Computer Engineering and University of Alberta, Edmonton, AB, Canada
    Analyst 136:4780-6. 2011
    ..Finally, GST-Gp48 was also immobilized onto magnetic beads that were successfully used to capture and pre-concentrate the host pathogen from suspension...
  6. ncbi Deuteration of a molecular probe for DNP hyperpolarization--a new approach and validation for choline chloride
    Hyla Allouche-Arnon
    Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Contrast Media Mol Imaging 6:499-506. 2011
    ..It is expected that the use of deuterated carbon-13 positions as reporting hyperpolarized nuclei will substantially expand the library of agents for DNP-MR...
  7. ncbi Targeted molecular probes for imaging atherosclerotic lesions with magnetic resonance using antibodies that recognize oxidation-specific epitopes
    Karen C Briley-Saebo
    Imaging Science Laboratory, Department of Radiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Circulation 117:3206-15. 2008
    ....
  8. ncbi Molecular probes for P2X7 receptor studies
    Hendra Gunosewoyo
    School of Chemistry, University of Sydney, NSW 2006 Australia
    Curr Med Chem 14:1505-23. 2007
    ..This article will review P2X7R ligands discovered to date, together with their biological activity and therapeutic potential...
  9. ncbi Surface modification of cetyltrimethylammonium bromide-capped gold nanorods to make molecular probes
    Chenxu Yu
    Department of Agricultural and Biological Engineering, Bindley Biosciences Center, Purdue University, 225 S University Street, West Lafayette, Indiana 47907, USA
    Langmuir 23:9114-9. 2007
    ..Gold nanorod molecular probes (GNrMPs) were fabricated by subsequent attachment of antibodies to the activated GNRs and were used to ..
  10. ncbi Application of RGD-containing peptides as imaging probes for alphavbeta3 expression
    Ingrid Dijkgraaf
    Technische Universitat Munchen, Department of Nuclear Medicine, Klinikum rechts der Isar, 81675 Munchen, Germany
    Front Biosci (Landmark Ed) 14:887-99. 2009
    ..In this review a brief overview on the currently used RGD-containing peptides as imaging probes for noninvasive visualization of alphavbeta3 expression using PET, SPECT, MRI, OI and US is given...
  11. ncbi Single-molecule and single-nanoparticle SERS: from fundamental mechanisms to biomedical applications
    X M Qian
    Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, 101 Woodruff Circle, Suite 2001, Atlanta, GA 30322, USA
    Chem Soc Rev 37:912-20. 2008
    ..These properties have raised new opportunities for multiplexed molecular diagnosis and in vivo Raman spectroscopy and imaging...
  12. pmc Superfamily-wide portrait of serine hydrolase inhibition achieved by library-versus-library screening
    Daniel A Bachovchin
    The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:20941-6. 2010
    ..Finally, we show that lead carbamate inhibitors can be optimized into pharmacological probes that inactivate individual SHs with high specificity in vivo...
  13. ncbi Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins
    Yusuke Jikumaru
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biosci Biotechnol Biochem 68:1461-6. 2004
    ..conjugate, a JA--dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA--binding proteins...
  14. ncbi Genotyping and characterization of the geographical distribution of tick-borne encephalitis virus variants with a set of molecular probes
    T V Demina
    Institute of Epidemiology and Microbiology, Scientific Center of Family Health and Human Reproduction Problems, SB RAMS, Irkutsk, Russia
    J Med Virol 82:965-76. 2010
    ..The fifth genotype includes 10 isolates, 1 of them, TBE886-84, was found earlier and recognized as unique [Zlobin et al. (2001b): Vopr Virusol 1:12-16 (Russian)]...
  15. ncbi A one-pot three-component radiochemical reaction for rapid assembly of 125I-labeled molecular probes
    Ran Yan
    Institute of Nuclear Medicine and Department of Chemistry, University College London, 235 Euston Road T 5, London NW1 2BU, UK
    J Am Chem Soc 135:703-9. 2013
    ..evaluation suggests that 5-[(125)I]iodo-1,2,3-triazoles are resistant to deiodination in vivo, both as small molecular probes and as antibody conjugates...
  16. pmc Tissue distribution studies of protein therapeutics using molecular probes: molecular imaging
    Simon Peter Williams
    Department of Biomedical Imaging, Genentech, Inc, South San Francisco, California 94080, USA
    AAPS J 14:389-99. 2012
    ....
  17. ncbi Activity-based probes for the study of proteases: recent advances and developments
    Sevnur Serim
    Center for integrated Protein Science Munich CIPS M, Lehrstuhl für Chemie der Biopolymere, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    ChemMedChem 7:1146-59. 2012
    ....
  18. pmc Biomarkers and molecular probes for cell death imaging and targeted therapeutics
    Bryan A Smith
    Department of Chemistry and Biochemistry, Notre Dame Integrated Imaging Facility, 236 Nieuwland Science Hall, University of Notre Dame, Notre Dame, IN 46556, USA
    Bioconjug Chem 23:1989-2006. 2012
    ..efforts over the past five years to identify useful biomarkers for dead and dying cells, and to develop molecular probes that target these biomarkers for optical, radionuclear, or magnetic resonance imaging...
  19. ncbi Characterization of subdomain IIA binding site of human serum albumin in its native, unfolded, and refolded states using small molecular probes
    Osama K Abou-Zied
    Department of Chemistry, Faculty of Science, Sultan Qaboos University, P O Box 36, Postal Code 123, Muscat, Sultanate of Oman
    J Am Chem Soc 130:10793-801. 2008
    ..The study was carried out in the absence and presence of small molecular probes using steady-state and time-resolved fluorescence measurements...
  20. ncbi In vivo near-infrared fluorescence imaging of matrix metalloproteinase activity after cerebral ischemia
    Jan Klohs
    Department of Experimental Neurology, Center for Stroke Research Berlin, Charite University Medicine Berlin, Chariteplatz 1, Berlin 10117, Germany
    J Cereb Blood Flow Metab 29:1284-92. 2009
    ..Our study shows that MMP activity after cerebral ischemia can be imaged noninvasively with NIRF using an MMP-activatable probe, which might be a useful tool to study MMP activity in the pathophysiology of the disease...
  21. ncbi Bacteriophage based probes for pathogen detection
    Amit Singh
    Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada
    Analyst 137:3405-21. 2012
    ..This review focuses on the description of pathogen detection approaches based on immobilized phage virions as well as pure recombinant RBPs. Specific advantages of RBP-based molecular probes are also discussed.
  22. pmc Characterizing mRNA interactions with RNA granules during translation initiation inhibition
    Chiara Zurla
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 6:e19727. 2011
    ..This methodology represents a valuable tool for future studies of mRNA trafficking and regulation within living cells...
  23. pmc High-potency olfactory receptor agonists discovered by virtual high-throughput screening: molecular probes for receptor structure and olfactory function
    Nicolas Triballeau
    Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS UMR 8601, Universite Paris Descartes, 45 Rue des Saints Peres, 75270 Paris Cedex 06, France
    Neuron 60:767-74. 2008
    ..Our virtual screening approach should be applicable to the identification of new bioactive molecules for probing the structure of chemosensory receptors and the function of chemosensory systems in vivo...
  24. pmc Molecular probes for imaging myelinated white matter in CNS
    Chunying Wu
    Department of Radiology and Neurosciences, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Med Chem 51:6682-8. 2008
    ..These studies suggested that these stilbene derivatives can be used as myelin-imaging probes to monitor myelin pathology in vivo...
  25. ncbi Activity-based probes: discovering new biology and new drug targets
    William P Heal
    Department of Chemistry, South Kensington Campus, Imperial College, London, SW7 2AZ, UK
    Chem Soc Rev 40:246-57. 2011
    ....
  26. pmc Molecular probes for diagnosis of clinically relevant bacterial infections in blood cultures
    Wendy L J Hansen
    Department of Medical Microbiology, Maastricht University Medical Center, P Debyelaan 25, Maastricht 6202 AZ, Netherlands
    J Clin Microbiol 48:4432-8. 2010
    ..Furthermore, this assay significantly reduced the time needed for identification. In conclusion, using pathogen-specific probes offers a faster alternative for pathogen detection and could improve the diagnosis of bloodstream infections...
  27. ncbi Site-specific labeling of proteins with small molecules in live cells
    Irwin Chen
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Curr Opin Biotechnol 16:35-40. 2005
    ....
  28. pmc DNA aptamers as molecular probes for colorectal cancer study
    Kwame Sefah
    Department of Chemistry, Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 5:e14269. 2010
    ..However, this can only be accomplished by developing high-affinity molecular probes with the ability to recognize specific markers associated with different tumors...
  29. pmc Hyperpolarized 89Y complexes as pH sensitive NMR probes
    Ashish K Jindal
    Advanced Imaging Research Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    J Am Chem Soc 132:1784-5. 2010
    ..Here we report the (89)Y chemical shift dependence upon pH for two hyperpolarized (89)Y(III) complexes and demonstrate how such complexes can be used as sensitive spectroscopy/imaging agents to measure pH...
  30. pmc Hyperpolarized lithium-6 as a sensor of nanomolar contrast agents
    Ruud B van Heeswijk
    Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Magn Reson Med 61:1489-93. 2009
    ..We conclude that the detection of submicromolar contrast agents using hyperpolarized NMR nuclei such as (6)Li may provide a novel avenue for molecular imaging...
  31. ncbi Enzymatic treatments reveal differential capacities for xylan recognition and degradation in primary and secondary plant cell walls
    Cecile Herve
    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK
    Plant J 58:413-22. 2009
    ..polymers, they can have differential capacities for xylan recognition in muro, and that the access of molecular probes and enzymes to xylan epitopes/ligands also varies between primary and secondary cell walls that are present ..
  32. ncbi Site-specific covalent labeling of proteins inside live cells using small molecule probes
    Souvik Chattopadhaya
    Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Bioorg Med Chem 17:981-9. 2009
    ..This approach has been demonstrated by the in vivo labeling of proteins in both bacterial and mammalian systems thereby making it potentially useful for future bioimaging applications...
  33. pmc Activity-based probes for rhomboid proteases discovered in a mass spectrometry-based assay
    Oliver Vosyka
    Center for Integrated Protein Science Munich, Lehrstuhl für Chemie der Biopolymere, Technische Universitat Munchen, 85354 Freising, Germany
    Proc Natl Acad Sci U S A 110:2472-7. 2013
    ..Overall, these probes represent valuable tools for rhomboid study, and the structural insights may facilitate future inhibitor design...
  34. ncbi Synthesis and evaluation of polyhydroxylated near-infrared carbocyanine molecular probes
    Zongren Zhang
    Department of Radiology, Washington University, St Louis, Missouri 63110, USA
    Org Lett 6:2067-70. 2004
    ..The structural framework of this molecule provides multivalent sites for labeling diverse molecules...
  35. pmc A cell-based computational modeling approach for developing site-directed molecular probes
    Jing Yu Yu
    Department of Pharmaceutical Sciences, University of Michigan College of Pharmacy, Ann Arbor, Michigan, United States of America
    PLoS Comput Biol 8:e1002378. 2012
    ....
  36. pmc Synthesis and properties of molecular probes for the rescue site on mutant cystic fibrosis transmembrane conductance regulator
    Bashar Alkhouri
    Department of Chemistry and Biology, Ryerson University, and Programme in Molecular Structure and Function, Research Institute, Hospital for Sick Children, 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada
    J Med Chem 54:8693-701. 2011
    ..To help elucidate the nature of the interactions between 1 and mutant CFTR, molecular probes based on the structure of 1 have been prepared...
  37. ncbi Strigolactone analogs as molecular probes in chasing the (SLs) receptor/s: design and synthesis of fluorescent labeled molecules
    Cristina Prandi
    Dipartimento di Chimica Università di Torino, Via P Giuria 7, 10127 Torino, Italy
    Mol Plant 6:113-27. 2013
    ..Knowledge of the putative receptor structure will boost the research on analogs of the natural substrates as required for agricultural applications...
  38. pmc Cell-based selection provides novel molecular probes for cancer stem cells
    Kwame Sefah
    Department of Chemistry, University of Florida, Gainesville, FL, USA
    Int J Cancer 132:2578-88. 2013
    ..The discovery of the molecular targets of these aptamers could reveal novel CSC biomarkers...
  39. ncbi Bivalent molecular probes for dopamine D2-like receptors
    Daniela Huber
    Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich Alexander University, Schuhstrasse 19, D 91052 Erlangen, Germany
    Bioorg Med Chem 20:455-66. 2012
    ..Obviously, the two pharmacophores occupy an orthosteric and an allosteric binding site rather than adopting a receptor-bridging binding mode...
  40. ncbi The use of a ditopic Gd(III) paramagnetic probe for investigating α-bungarotoxin surface accessibility
    Andrea Bernini
    Dipartimento di Biotecnologie, Universita di Siena, I 53100 Siena, Italy
    J Inorg Biochem 112:25-31. 2012
    ..These features make Gd(2)L7 a very suitable probe for investigating protein surface accessibility of complex protein systems...
  41. pmc Use of cell-SELEX to generate DNA aptamers as molecular probes of HPV-associated cervical cancer cells
    Jessica C Graham
    Department of Environmental and Occupational Medicine, Robert Wood Johnson, Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey, United States of America
    PLoS ONE 7:e36103. 2012
    ..In the present study, we used live cell-based SELEX to identify DNA aptamers which recognize cell surface differences between HPV-transformed cervical carcinoma cancer cells and isogenic, nontumorigenic, revertant cell lines...
  42. ncbi Synthesis and biological evaluation of molecular probes based on the 9-methylstreptimidone derivative DTCM-glutarimide
    Eisuke Ota
    Department of Chemistry, Faculty of Science and Technology, Keio University, Hiyoshi 3 14 1, Kohoku Ku, Yokohama 223 8522, Japan
    Bioorg Med Chem Lett 22:164-7. 2012
    b>Molecular probes based on 3-[(dodecylthiocarbonyl)methyl]glutarimide (DTCM-glutarimide) were synthesized and assessed for inhibitory activity against LPS-induced NO production...
  43. ncbi Fabrication of nano-indented cavities on Au for the detection of chemically-adsorbed DTNB molecular probes through SERS effect
    Chia Wei Chang
    Department of Materials Science and Engineering, National Cheng Kung University, No 1, University Road, Tainan 70101, Taiwan
    J Colloid Interface Sci 358:384-91. 2011
    ..SERS enhancement factor on a specific NTB/nAu could be increased to 2.1×10(6)...
  44. ncbi Behavior of a photoactivatable analog of cholesterol, 6-photocholesterol, in model membranes
    Evan A Mintzer
    Department of Chemistry and Biochemistry, Queens College of the City University of New York, Flushing, 13367, USA
    FEBS Lett 510:181-4. 2002
    ..The results suggest that this analog is a suitable photoprobe of cholesterol...
  45. ncbi Dual-targeted molecular probes for cancer imaging
    Zhaofei Liu
    Medical Isotopes Research Center, Peking University, Beijing 100191, China
    Curr Pharm Biotechnol 11:610-9. 2010
    ..Receptors that are overexpressed in tumors have been potential targets for cancer imaging using targeted molecular probes. Since many cancer types simultaneously overexpress several receptors, the design of dual receptor-targeted ..
  46. pmc Organic synthesis toward small-molecule probes and drugs
    Stuart L Schreiber
    Howard Hughes Medical Institute, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 108:6699-702. 2011
    ..This perspective article describes new roles that modern organic chemistry will need to play in overcoming this challenge...
  47. ncbi Targeted molecular imaging of antigen OC183B2 in ovarian cancers using MR molecular probes
    Guangnan Quan
    Department of Radiology, Peking University People s Hospital, Beijing, China
    Acad Radiol 17:1468-76. 2010
    ..This study was designed to develop a novel magnetic resonance (MR) probe for the antigen OC183B2 in ovarian cancer cells and investigate its imaging features in vitro and in vivo...
  48. pmc Hot spot analysis for driving the development of hits into leads in fragment-based drug discovery
    David R Hall
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
    J Chem Inf Model 52:199-209. 2012
    ..The method places small molecular probes, the size of organic solvents, on a dense grid around the protein and identifies the hot spots as consensus ..
  49. pmc Real time monitoring of biomaterial-mediated inflammatory responses via macrophage-targeting NIR nanoprobes
    Jun Zhou
    Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA
    Biomaterials 32:9383-90. 2011
    ..Our results support that FR-targeting NIR nanoprobes can be used to monitor and quantify the extent of macrophage recruitment and the degree of an implants' biocompatibility in real time...
  50. pmc Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells
    D J Gillooly
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    EMBO J 19:4577-88. 2000
    ....
  51. ncbi A modified staining technique for arbuscular mycorrhiza compatible with molecular probes
    M Pitet
    Plant Protection IRTA, Ctra de Cabrils Km 2, E 08348 Cabrils, Barcelona, Spain
    Mycorrhiza 19:125-31. 2009
    ..The extraction and amplification process was reliable up to 3 days after staining. A week after staining, the amplification was not dependable and after 2 weeks there was no amplification from stained material...
  52. ncbi Site-specific in vitro and in vivo incorporation of molecular probes to study G-protein-coupled receptors
    Kelly A Daggett
    Laboratory of Molecular Biology and Biochemistry, Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    Curr Opin Chem Biol 15:392-8. 2011
    ..This method should allow site-specific labeling of GPCRs with various molecular probes to facilitate cell-based studies of protein-protein or protein-ligand interactions and the visualization of ..
  53. pmc The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
    Laurie F Mottram
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Org Lett 8:581-4. 2006
    ..2) and 4-carboxy-Pennsylvania Green (pK(a) approximately 4.8) revealed that only Pennsylvania Green was highly fluorescent in acidic early and recycling endosomes within living mammalian cells...
  54. pmc Fluorescence lifetime imaging of activatable target specific molecular probes
    Raphael Alford
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1088, USA
    Contrast Media Mol Imaging 5:1-8. 2010
    ..We hypothesized that these molecular probes could be utilized to accurately report on cellular internalization with fluorescence lifetime imaging (FLI)...
  55. pmc Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes
    Sarah R Hanson
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 129:7266-7. 2007
  56. pmc Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
    Mikhail Y Berezin
    Department of Radiology, Washington University, St Louis, Missouri 63110, United States
    Biochemistry 50:2691-700. 2011
    ..The correlation provides a guideline for dye selection in optical imaging and a method to verify if a certain dye is optimal for a specific molecular imaging application...
  57. pmc Self-assembled gold nanoparticle molecular probes for detecting proteolytic activity in vivo
    C Jenny Mu
    Harvard MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA
    ACS Nano 4:1511-20. 2010
    ..These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model...
  58. pmc 64Cu-labeled alpha-melanocyte-stimulating hormone analog for microPET imaging of melanocortin 1 receptor expression
    Zhen Cheng
    Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, California 94305 5344, USA
    Bioconjug Chem 18:765-72. 2007
    ..This study demonstrates that 64Cu-DOTA-NAPamide is a promising molecular probe for alpha-MSH receptor positive melanoma PET imaging as well as MC1R expression imaging in living mice...
  59. ncbi Dynamic imaging of protease activity with fluorescently quenched activity-based probes
    Galia Blum
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, California 94305, USA
    Nat Chem Biol 1:203-9. 2005
    ..This probe is used to monitor real-time protease activity in live human cells with fluorescence microscopy techniques as well as standard biochemical methods...
  60. pmc ESR evidence for 2 coexisting liquid phases in deeply supercooled bulk water
    D Banerjee
    Department of Physics, Indian Institute of Science, Bangalore 560 012, India
    Proc Natl Acad Sci U S A 106:11448-53. 2009
    ..Nature 398:492-494]...
  61. pmc Development of molecular probes for imaging sigma-2 receptors in vitro and in vivo
    Robert Henry Mach
    Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cent Nerv Syst Agents Med Chem 9:230-45. 2009
    ....
  62. ncbi Probing the ligand-induced conformational change in HLA-DR1 by selective chemical modification and mass spectrometric mapping
    Gregory J Carven
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 44:13625-37. 2005
    ....
  63. ncbi Multivalent carbocyanine molecular probes: synthesis and applications
    Yunpeng Ye
    Department of Radiology, Washington University, St Louis, Missouri 63110, USA
    Bioconjug Chem 16:51-61. 2005
    ..These results suggest that multivalent molecular beacons are useful for assessing the beneficial effects of multivalency and for optimizing the biological and chemical properties of tissue-specific molecular probes.
  64. ncbi Development of molecular probes for the identification of extra interaction sites in the mid-gorge and peripheral sites of butyrylcholinesterase (BuChE). Rational design of novel, selective, and highly potent BuChE inhibitors
    Giuseppe Campiani
    Dipartimento Farmaco Chimico Tecnologico, Via Aldo Moro, and European Research Centre for Drug Discovery and Development NatSynDrugs, Universita di Siena, 53100 Siena, Italy
    J Med Chem 48:1919-29. 2005
    ..The novel inhibitors, bearing postulated key features, validated the hypothesis of the presence of extra interaction sites within the hBuChE active site gorge...
  65. ncbi In vivo resolution of multiexponential decays of multiple near-infrared molecular probes by fluorescence lifetime-gated whole-body time-resolved diffuse optical imaging
    Walter Akers
    Optical Radiology Lab, Department of Radiology, Washington University School of Medicine, St Louis, MO 63146, USA
    Mol Imaging 6:237-46. 2007
    ..830 nm) wavelengths were used to image the distinct fluorescence lifetime distribution of the fluorescent molecular probes in the mouse cancer model...
  66. ncbi Ultrasensitive protein-DNA binding assays
    Victor Pavski
    Environmental Health Sciences Program, Department of Public Health Sciences, University of Alberta, Alberta, Edmonton, Canada T6G 2G3
    Curr Opin Biotechnol 14:65-73. 2003
    ..Recent advances have been made in protein-DNA binding assays using capillary electrophoresis, laser-induced fluorescence, fluorescence polarization, molecular beacons, and affinity chromatography...
  67. pmc CS-SELEX generates high-affinity ssDNA aptamers as molecular probes for hepatitis C virus envelope glycoprotein E2
    Fang Chen
    State Key Laboratory of Virology, Department of Immunology, Wuhan University School of Medicine, Wuhan, People s Republic of China
    PLoS ONE 4:e8142. 2009
    ..data demonstrate that the newly selected ssDNA aptamers, especially aptamer ZE2, hold great promise for developing new molecular probes, as an early diagnostic reagent for HCV surface antigen, or a therapeutic drug specifically for HCV.
  68. pmc Activatable molecular probes for cancer imaging
    Seulki Lee
    Laboratory of Molecular Imaging and Nanomedicine LOMIN, National Institute of Biomedical Imaging and Bioengineering NIBIB, National Institutes of Health NIH, 31 Center Dr, Suite 1C14, Bethesda, MD 20892 2281, USA
    Curr Top Med Chem 10:1135-44. 2010
    The development of highly sensitive and specific molecular probes for cancer imaging still remains a daunting challenge...
  69. ncbi Molecular probes of the vestibular nerve. II. Characterization of neurons in Scarpa's ganglion to determine separate populations within the nerve
    Golda Anne Kevetter
    Department of Otolaryngology, University of Texas Medical Branch, 301 University Dr, Galveston, TX 77555 1043, USA
    Brain Res 928:18-29. 2002
    ..This study has provided an anatomical instrument (in addition to intracellular physiological methods) to study separate populations of vestibular afferents...
  70. ncbi Strategies and molecular probes to investigate the role of cytochrome P450 in drug metabolism: focus on in vitro studies
    M Teresa Donato
    Unit of Experimental Hepatology, Research Centre, University Hospital La Fe and Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
    Clin Pharmacokinet 42:153-78. 2003
    ..Primary cultured hepatocytes are still the unique in vitro model that allows global examination of the inductive potential of a drug...
  71. pmc Nanometer-scale mapping and single-molecule detection with color-coded nanoparticle probes
    Amit Agrawal
    Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 101 Woodruff Circle, Suite 2001, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 105:3298-303. 2008
    ....
  72. ncbi Photophysical aspects of molecular probes near nanostructured gold surfaces
    Sujit Kumar Ghosh
    Department of Chemistry, Raidighi College, South 24 Parganas, Raidighi, 743383, India
    Phys Chem Chem Phys 11:3831-44. 2009
    ..important to elucidate the particle size and distance dependence on the interaction between excited molecular probes and the gold nanoparticles...
  73. ncbi Single quantum dot-micelles coated with silica shell as potentially non-cytotoxic fluorescent cell tracers
    Zhivko Zhelev
    On site Sensing and Diagnosis Research Laboratory, National Institute of Advanced Industrial Science and Technology, AIST Kyushu, 807 1 Shuku machi, Tosu 841 0052, Japan
    J Am Chem Soc 128:6324-5. 2006
    ..The nanoparticles show ability for intracellular delivery and are noncytotoxic during long-term incubation with viable cells in the absence of light exposure, which makes them appropriate for cell tracing and drug delivery...
  74. ncbi Effects of solvent polarity and solvent viscosity on the fluorescent properties of molecular rotors and related probes
    M A Haidekker
    Department of Biological Engineering, University of Missouri Columbia, Columbia, MO 65211, USA
    Bioorg Chem 33:415-25. 2005
    ..Coumarins, on the other hand, did not show viscosity sensitivity. This study shows the unique suitability of molecular rotors as fluorescent viscosity sensors...
  75. ncbi A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C
    Fang Yuan
    Department of Pathology, and Microbiology and Immunology, Stanford School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    J Am Chem Soc 128:5616-7. 2006
    ..We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members...
  76. pmc Allele quantification using molecular inversion probes (MIP)
    Yuker Wang
    ParAllele BioScience, 7300 Shoreline Boulevard, South San Francisco, CA 94080, USA
    Nucleic Acids Res 33:e183. 2005
    ..We have shown the ability of MIP at this multiplex level to provide copy number measurements while obtaining the allele information. In addition we have demonstrated a proof of principle for copy number analysis in FFPE samples...
  77. ncbi Activity based probes for proteases: applications to biomarker discovery, molecular imaging and drug screening
    Marko Fonovic
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    Curr Pharm Des 13:253-61. 2007
    ..Specifically we focus on application of this technique for biomarker discovery, in vivo imaging and drug screening...
  78. pmc Analysis of molecular inversion probe performance for allele copy number determination
    Yuker Wang
    Affymetrix Inc, Shoreline Blvd, South San Francisco, CA 94080, USA
    Genome Biol 8:R246. 2007
    ....
  79. ncbi Activity-based probes that target diverse cysteine protease families
    Daisuke Kato
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, California, USA
    Nat Chem Biol 1:33-8. 2005
    ..Biochemical studies using these reagents highlight their overall utility and provide insight into the biochemical functions of members of these protease families...
  80. pmc Application of Lifeact reveals F-actin dynamics in Arabidopsis thaliana and the liverwort, Marchantia polymorpha
    Atsuko Era
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Plant Cell Physiol 50:1041-8. 2009
    ..Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages...
  81. ncbi Bis-imidazoles as molecular probes for peripheral sites of the zinc endopeptidase of botulinum neurotoxin serotype A
    Isidro Merino
    Computer Aided Molecular Design Laboratory, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Bioorg Med Chem 14:3583-91. 2006
    ..This peripheral site enables the use of the bivalence approach to improve our previously reported small-molecule inhibitors that were developed according to the zinc-coordination approach...
  82. ncbi Small molecules, big players: the National Cancer Institute's Initiative for Chemical Genetics
    Nicola Tolliday
    Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
    Cancer Res 66:8935-42. 2006
    ..This report outlines how the ICG functions, how researchers can take advantage of its screening, chemistry and informatic capabilities, and provides a brief summary of some of the many important research findings...
  83. ncbi Lighting up tumors with receptor-specific optical molecular probes
    Samuel Achilefu
    Department of Radiology, Washington University School of Medicine, 4525 Scot Avenue, St Louis, MO 63110, USA
    Technol Cancer Res Treat 3:393-409. 2004
    ..In this era of molecular medicine, molecular probes that respond to, or target molecular processes are indispensable...
  84. pmc Semi-automated, single-band peak-fitting analysis of hydroxyl radical nucleic acid footprint autoradiograms for the quantitative analysis of transitions
    Keiji Takamoto
    Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Nucleic Acids Res 32:E119. 2004
    ..The utility of this new analysis approach is illustrated by its application to the ion-meditated folding of a large RNA molecule...
  85. pmc Dual FRET molecular beacons for mRNA detection in living cells
    Philip J Santangelo
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Nucleic Acids Res 32:e57. 2004
    ..2. We further revealed intriguing details of K-ras and survivin mRNA localization in living cells. The dual FRET molecular beacons approach provides a novel technique for sensitive RNA detection and quantification in living cells...
  86. ncbi Site-3 sea anemone toxins: molecular probes of gating mechanisms in voltage-dependent sodium channels
    Jaime J Smith
    Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York, 3435 Main St Buffalo, NY 14214, USA
    Toxicon 49:159-70. 2007
    ..Because of their ability to discriminate among channel isoforms, and their high degree of structural conservation, these toxins could serve as important lead compounds for future pharmaceutical design...
  87. ncbi NIH Molecular Libraries Initiative
    Christopher P Austin
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 306:1138-9. 2004
  88. pmc Mechanisms and molecular probes of sirtuins
    Brian C Smith
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Chem Biol 15:1002-13. 2008
    ..deacetylase mechanism of sirtuins and how this information might be employed to develop inhibitors and other molecular probes for therapeutic and basic research applications...
  89. ncbi Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes
    Alicia B Berger
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    Mol Cell 23:509-21. 2006
    ..Our data suggest that caspase-7 activation proceeds through a previously uncharacterized intermediate that is formed without cleavage of the intact zymogen...
  90. ncbi Constrained azacyclic analogues of the immunomodulatory agent FTY720 as molecular probes for sphingosine 1-phosphate receptors
    Stephen Hanessian
    Department of Chemistry, Universite de Montreal, PO Box 6128, Station Centre Ville, Montreal, Que, Canada H3C 3J7
    Bioorg Med Chem Lett 17:491-4. 2007
    ..The phosphates may be useful to explore the biology and binding site of these receptors...
  91. ncbi Specificity of aza-peptide electrophile activity-based probes of caspases
    K B Sexton
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 940305, USA
    Cell Death Differ 14:727-32. 2007
    ..This study also highlights the importance of confirming the specificity of covalent protease inhibitors in crude proteomes using reagents such as the ABPs described here...
  92. ncbi Open access chemical and clinical probes to support drug discovery
    Aled M Edwards
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    Nat Chem Biol 5:436-40. 2009
    ..Chemical biologists could play a central role in this effort...
  93. pmc Highly multiplexed molecular inversion probe genotyping: over 10,000 targeted SNPs genotyped in a single tube assay
    Paul Hardenbol
    ParAllele BioScience, Inc, South San Francisco, California 94080, USA
    Genome Res 15:269-75. 2005
    ..The results of this study demonstrate the suitability of this technology for comprehensive association studies that use targeted SNPs in indirect linkage disequilibrium studies or that directly screen for causative mutations...
  94. pmc A crowdsourcing evaluation of the NIH chemical probes
    Tudor I Oprea
    University of New Mexico Center for Molecular Discovery, University of New Mexico School of Medicine, Albuquerque, New Mexico, USA
    Nat Chem Biol 5:441-7. 2009
    ..We crowdsourced the Molecular Libraries and Imaging initiative output to 11 experts, who expressed medium or high levels of confidence in 48 of these 64 probes...
  95. ncbi Molecular rotors--fluorescent biosensors for viscosity and flow
    Mark A Haidekker
    Department of Biological Engineering, University of Missouri Columbia, Columbia, MO 65211, USA
    Org Biomol Chem 5:1669-78. 2007
    ..Molecular rotors are therefore emerging as new biosensors for both bulk and local microviscosity, and for flow and fluid shear stress on a microscopic scale and with real-time response...
  96. doi Molecular biology. Industrial-style screening meets academic biology
    Jocelyn Kaiser
    Science 321:764-6. 2008
  97. ncbi Improving the visualization of fluorescently tagged nanoparticles and fluorophore-labeled molecular probes by treatment with CuSO(4) to quench autofluorescence in the rat inner ear
    Ya Zhang
    Department of Otolaryngology, University of Tampere, Tampere, Finland
    Hear Res 269:1-11. 2010
    Fluorescent tags and fluorophore-conjugated molecular probes have been extensively employed in histological studies to demonstrate nanoparticle distribution in inner ear cell populations...
  98. ncbi Investigating nanostructuring within imidazolium ionic liquids: a thermodynamic study using photochromic molecular probes
    Simon Coleman
    Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland
    J Phys Chem B 113:15589-96. 2009
    ..The result of such reordering and dispersion of polar regions reduces solvent-solute interactions which increases the rate of MC(BSP) to BSP relaxation...
  99. ncbi Divergent synthesis of multifunctional molecular probes to elucidate the enzyme specificity of dipeptidic gamma-secretase inhibitors
    Haruhiko Fuwa
    Laboratory of Biostructural Chemistry, Graduate School of Life Sciences, Tohoku University, Tsutsumidori Amamiya, Aoba ku, Sendai, Japan
    ACS Chem Biol 2:408-18. 2007
    Divergent synthesis of multifunctional molecular probes based on caprolactam-derived dipeptidic gamma-secretase inhibitors (GSIs), Compound E (CE) and LY411575 analogue (DBZ), was efficiently accomplished by means of Cu(I)-catalyzed azide/..
  100. ncbi Detection of DNA hybridization using induced fluorescence resonance energy transfer
    W Mathias Howell
    Center for Genomics and Bioinformatics, Karolinska Institute, Stockholm, Sweden
    Methods Mol Biol 335:33-41. 2006
    ..Because the interaction of the donor dye is reversible and dependent on the presence of double-stranded DNA, iFRET is extremely useful and herein demonstrated in the generation of DNA melting curves...
  101. ncbi Multi-fluorophore fluorescence resonance energy transfer for probing nucleic acids structure and folding
    Juewen Liu
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL, USA
    Methods Mol Biol 335:257-71. 2006
    ..Instead, fluorophore-to-fluorophore distance should be used...

Research Grants75

  1. Phage Display for Improved Peptide-based Tumor Targeting and Imaging Agents
    Susan L Deutscher; Fiscal Year: 2013
    ..New peptide-based molecular probes to facilitate cancer imaging are rapidly evolving due to implementation of bacteriophage (phage) display ..
  2. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..can function as small artificial cell surface receptors;when linked to motifs that bind drugs or molecular probes, they can shuttle these compounds into cells and tissues via endocytosis...
  3. High throughput screen for selective inhibitors of cyclophilin B
    Richard J Bram; Fiscal Year: 2013
    ..These compounds will ultimately lead to establishing a novel approach for therapeutic treatment of GBM. In addition, they will have great utility as molecular probes to enhance studies defining the critical functions of CypB.
  4. Development of New Molecular Probes for Diagnostic Imaging of Atherosclerosis
    KAREN CATHERIN BRILEY SAEBO; Fiscal Year: 2010
    ....
  5. Mechanisms of Postoperative Ileus
    YVETTE FRANCE TACHE; Fiscal Year: 2012
    ..to achieve these aims will dwell on new technical advances recently made in our laboratories including key molecular probes to assess gene expression of rodent CRF ligands and CRF1 and CRF2 receptors and novel receptor variants that ..
  6. Molecular Imaging Probes for Reporting on Vascular Oxidative Response
    Alexei A Bogdanov; Fiscal Year: 2013
    ..imaging probes: enzyme reporting, dismutase mimic properties and stability in vitro;3) perform testing of molecular probes for imaging oxidative response in vivo...
  7. Challenges in beta-Lactamase Mediated Resistance
    Robert A Bonomo; Fiscal Year: 2013
    ..In this proposal we will continue our work with boronic acid transition state inhibitors (BATSIs) as molecular probes to target the ES SHV of K. pneumoniae and the AmpC of P. aeruginosa, PDC-3...
  8. Molecular probes for a vOTU from CCHFV using a fluorogenic peptide
    SCOTT DUSAN PEGAN; Fiscal Year: 2013
    ..b>Molecular probes initially identified for the vOTU from CCHFV will be subsequently optimized using secondary, orthogonal, and ..
  9. Yeast Based Assays for Chemical Screens Against SARS-CoV Targets
    DANIEL A contact ENGEL; Fiscal Year: 2010
    ..studies will develop a new high throughput paradigm to identify novel pharmaceuticals that will be used as molecular probes of coronavirus function...
  10. Analysis of Redox Modulated Signaling Networks in Response to Ionizing Radiation
    Cristina Furdui; Fiscal Year: 2013
    ..stimulation with growth factors with millisecond time resolution, iii] first time use of highly specific molecular probes for the detection of sulfenic acid containing proteins as key intermediates in redox signaling, and iv] ..
  11. Molecular Probes of the Mechanisms of Cytocrhome P450
    JOHN TAYLOR GROVES; Fiscal Year: 2013
    ..abstract_text> ..
  12. Cell Death Regulation by Pro-Apoptotic BOK During Hematopoiesis
    Samuel G Katz; Fiscal Year: 2013
    ..that retain the specificity of native BH3 death ligands and directly activate BOK will serve as unique molecular probes and prototype therapeutics to investigate and modulate BCL-2 family interactions in vitro and in vivo...
  13. High throughput assay for fibrin-binding probes
    PETER D CARAVAN; Fiscal Year: 2013
    ..The ultimate goal is to develop new molecular probes to image fibrin, its formation, and its dissolution...
  14. Quantitative Optical Imaging of Molecular Processes in Preclinical Cancer Models
    WALTER JOHN AKERS; Fiscal Year: 2013
    ..principles will be applied to develop complementary optical imaging methods with conventional and activatable molecular probes. The practical aspects of animal models of human cancer, including human cell and tissue xenografts, ..
  15. New Thoughts on Parietal Epithelial Cells
    Stuart Shankland; Fiscal Year: 2010
    ..and PEC disease in Ksp-EYFP mice (PECs are green) injected with different types and sizes of fluorescent molecular probes as tracers...
  16. Characterizing the effects of Mouse Maturation on the Expression of Acute Renal F
    Richard A Zager; Fiscal Year: 2010
    ..of factors, including relative cost, the heterogeneity of available strains, the availability of protean molecular probes for studying this species, and their utility for induction of specific genetic alterations, such as whole ..
  17. Development of High Affinity Analogues of Brevenal: Novel Molecular Probes for Ne
    Mary C Wolf; Fiscal Year: 2011
    ..PDF created with pdfFactory trial version www.pdffactory.com ..
  18. MOLECULAR PROBES FOR STEROID RECEPTORS
    John A Katzenellenbogen; Fiscal Year: 2012
    ..1 Description, ..
  19. Mechanisms of permeation and gating of voltage-sensing domains
    Francesco Tombola; Fiscal Year: 2013
    ..of proton permeation through the VSD and the mechanism of voltage sensing, using new Hv1 blockers as molecular probes, and 3) investigating the mechanisms of subunit coupling and gating modulation by accessory proteins...
  20. Fission Yeast as a HTS Platform for New Molecular Probes of HIV-1 VPR-Medicated A
    Richard Yuqi Zhao; Fiscal Year: 2010
    ..ability of Vpr to interrupt host cell cycle G2-M transition and to induce apoptosis, identification of new molecular probes against these Vpr activities could also have potential utility in new drug designs against cancerous cells...
  21. Novel modalities for assessing the cortical tissue-electrode interface
    Jeffrey R Capadona; Fiscal Year: 2010
    ..Such "molecular probes" have been readily employed to study cancer growth and inflammatory disease due to their ability to detect ..
  22. High-throughput assay development for molecular probes targeting the ULK1 kinase
    DEREK RONALD DUCKETT; Fiscal Year: 2013
    ....
  23. Mechanism-based small molecule epigenetic modulators: Targeting specific HDACs
    Nicos A Petasis; Fiscal Year: 2010
    ..characterize the functions and properties of these molecules and establish the basis for developing new molecular probes to study HDAC function, including imaging agents and new leads for potential therapeutics for a number of ..
  24. Development of Assays for Inhibitors of LysRS in Mast Cell Activation
    Min Guo; Fiscal Year: 2013
    ..lead compounds against a series of mechanistic screens will drive the discovery of LysRS novel and selective molecular probes for its non-canonical function in mast cell activation, with an ultimate goal of developing unique anti-..
  25. Disrupting Biofilm Formation to Improve TB Drug Treatment
    Randall J Basaraba; Fiscal Year: 2013
    ..In a series of mechanistic studies, we will use biotinylated, anti- biofilm compounds as molecular probes to identify Mtb specific virulence factors unique to the drug-tolerant phenotype...
  26. Local anesthetic receptor in peripheral Na+ channels
    GING K WANG; Fiscal Year: 2013
    ..7 and Nav1.8 Na+ channels. Toward this goal, we plan to apply above pain therapeutics as molecular probes for their receptors in wild-type and inactivation-deficient Nav1.7 and Nav1.8 Na+ channels...
  27. High Throughput Screening to Discover Chemical Probes of ASK1
    LOUIS DANIEL SCAMPAVIA; Fiscal Year: 2013
    ..we submit that the successful completion of our studies will identify a series of new, potent and selective molecular probes that will help define the role(s) that ASK1 plays in normal physiological processes as well as in disease ..
  28. Modifications of host RNA polymerase during bacteriophage development
    KONSTANTIN V SEVERINOV; Fiscal Year: 2013
    ..with different subunits of RNAP and affect different stages of the transcription cycle will provide novel molecular probes to better understand RNAP mechanism and regulation and to uncover RNAP sites that can be targets for drug ..
  29. A Total Synthesis of Gambierol Using an Epoxide-Opening Cascade Approach
    DENISE ANN COLBY; Fiscal Year: 2010
    ..In addition, ladder polyethers and their derivatives have found use as molecular probes to elucidate the structure and function of voltage-gated ion channels, which ultimately leads to the improved ..
  30. Antibodies to Define Molecular Mechanisms of Hypertriglyceridemia
    Stephen G Young; Fiscal Year: 2010
    ..and Specific Challenge Topic 06- HL-102, which is to develop high affinity/high specificity targeted molecular probes for molecular imaging of cardiovascular and pulmonary disease targets...
  31. Molecular Probes for Biomembrane Recognition
    Bradley D Smith; Fiscal Year: 2013
    ..The power of this new imaging technology will be employed to answer questions concerning the ability of the tumor vascular-penetrating peptide iRGD to promote nanoparticle entry into tumors. ..
  32. Synthetic Cell Surface Receptors for Anticancer Drug Delivery
    Blake Peterson; Fiscal Year: 2012
    ..We will optimize the delivery of doxorubicin and related molecular probes into cancer cells in vitro to validate a fundamentally new concept: compounds that engage a defined membrane ..
  33. Use of Silver Nanostructures on Microtransponders in Ultra-sensitive Assay
    Wlodek Mandecki; Fiscal Year: 2011
    ..Acceptance of nanotechnology-based multiplex assays on microtransponders by researchers and clinicians could initiate new methods in disease diagnosis and treatment, drug discovery, and biological or environmental research. ..
  34. Developing Myxovirus Inhibitors with Expanded Pathogen Target Range
    Richard K Plemper; Fiscal Year: 2013
    ..conserved among related paramyxovirus L proteins, photoreactive analogs will be employed as innovative molecular probes to optimize a folding-competent L fragment identified in pilot studies for co- crystallization...
  35. PROFILING AUTOANTIGENS IN UVEITIS
    Wei Li; Fiscal Year: 2010
    ..Autoantibodies in patient serum can be used as molecular probes to identify autoantigens by various methods...
  36. WHOLE-BODY SMALL-ANIMAL PHOTOACOUSTIC-ULTRASONIC COMPUTED TOMOGRAPHY
    Mark A Anastasio; Fiscal Year: 2013
    ..With the aid of functionalized contrast agents (molecular probes), PACT can also permit molecular imaging...
  37. RECOVERY OF OPTICAL ABSORPTION COEFFICIENT IN QUANTITATIVE PHOTOACOUSTIC IMAGING
    Lihong Wang; Fiscal Year: 2011
    ..With the aid of functionalized contrast agents (molecular probes), PAI can also provide molecular imaging...
  38. In Viro Detection of Neoplasia in the Digestive Tract
    Thomas D Wang; Fiscal Year: 2012
    ..Peptides have been chosen for use as molecular probes because of their clonal diversity, small size, and minimal immunogenicity, and are well-suited for clinical ..
  39. Multimodality Imaging of GI Cancers for Diagnosis and Directed Therapy
    Christopher H Contag; Fiscal Year: 2013
    ..systems and state-of-the-art miniaturized sensors for high resolution/high sensitivity detection of molecular probes directed at markers of cancer. The probe chemistries are well-established and based on existing targets...
  40. Intraoperative real time breast cancer margin assessment with nonlinear microscop
    James G Fujimoto; Fiscal Year: 2013
    ..such as 3D imaging, multiple contrast channels using selected endogenous or exogenous fluorophores and molecular probes, in addition to image processing and display methods to enhance diagnostic performance or provide ..
  41. A Multi-user Super Resolution Microscope for Developmental Biology
    SAMUEL M KUNES; Fiscal Year: 2010
    ..In combination with a wide array of multi-color fluorescent molecular probes, these are powerful technologies that open vast new areas of inquiry...
  42. UAB Core Center for Basic Skeletal Research (CCBSR)
    Selvarangan Ponnazhagan; Fiscal Year: 2010
    ....
  43. MOLECULAR PROBES AND METHODS FOR FLUORESCENCE LIFETIME TOMOGRAPHY AND MICROSCOPY
    Samuel Achilefu; Fiscal Year: 2012
    ..To realize fully the potential of FLT approach, there is a compelling need to develop NIR molecular probes with diverse and biologically sensitive FLTs...
  44. Temperature Modulated-Fluorescence Tomography: A New Modality for Cancer Imaging
    TIFFANY KWONG; Fiscal Year: 2013
    ..There are two key components to this highly innovative approach: a) Temperature sensitive florescent molecular probes (thermo-dots) and b) High Intensity Focused Ultrasound (HIFU) based modulation of tissue temperature (only 3-..
  45. Monodispersed Biocompatible Magnetic Nanoprobes for Improved Diagnostic Imaging
    KANNAN MANJAPRA KRISHNAN; Fiscal Year: 2011
    ..Thus, there is a critical need to develop magnetic molecular probes that, unlike contrast agents, can be directly imaged, irrespective of the surrounding tissue, and can be ..
  46. An interdisciplinary approach to elucidate mechanisms of muscle lipotoxicity
    GREGORY PAUL ROTH; Fiscal Year: 2011
    ..The long-term goal of this project is to identify new genes, pathways, and molecular probes relevant to muscle lipotoxicity, serving as a valuable hypothesis-generating resource for the field and ..
  47. High Resolution Multiplexed Fluorescence Tomography
    MARK JONATHAN NIEDRE; Fiscal Year: 2013
    ..is to develop a highly novel fluorescence tomographic imager for high- resolution multiplexed imaging of molecular probes and red-shifted fluorescent proteins in whole animals in vivo...
  48. HTS for Caspase-8 selective TRAIL Sensitizers
    Kristiina Vuori; Fiscal Year: 2013
    ..for highly automated HTS, and subsequently to screen the ~330,600 MLSMR collection with a view to identifying molecular probes that may assist in the interrogation of signaling pathways relevant to human disease and to potentially ..
  49. Inflammation and the esophageal tumor microenvironment
    TIMOTHY CRAGIN WANG; Fiscal Year: 2013
    ..lesions facilitated by the development of mouse upper endoscopy, non-invasive imaging technologies and molecular probes (tracking inflammatory/immune cells in the esophageal cancer microenvironment) by Dr...
  50. Regulation of Vascular Smooth Muscle Cell Proliferation
    Hana Totary Jain; Fiscal Year: 2010
    ..We will test new drug combinations in animal model in which resistance to rapamycin's antiproliferative effect was observed. ..
  51. New Methods for the Expedited Synthesis of C11 and F18 PET Tracers
    Salvatore D Lepore; Fiscal Year: 2013
    ..These molecular probes have become indispensable for understanding the mechanisms of both normal brain function and brain disorders...
  52. Development of Second Messenger, Trafficking, and Functional Assays for GPR119
    PATRICIA HELEN MCDONALD; Fiscal Year: 2012
    ..of novel high throughput compatible cell-based functional assay to identify GPR119 selective ligands that can be used as molecular probes to interrogate GPR119 receptor function in normal physiological processes and disease states.
  53. Toxins as molecular probes of pain transduction mechanisms
    JEREMIAH OSTEEN; Fiscal Year: 2013
    ....
  54. Reactivating Apoptosis in Refractory Cancer by Selective Targeting of MCL-1
    Michelle Stewart; Fiscal Year: 2010
    ..MCL-1 overexpressing cancers, a novel chemical biology approach will be deployed to develop selective MCL-1 inhibitors for use as molecular probes to study the biology of MCL-1 and as prototype therapeutics to combat MCL-1 driven cancer.
  55. High-throughput portable software for fragment-based drug design
    Sandor Vajda; Fiscal Year: 2011
    ..The method moves molecular probes - small organic molecules containing various functional groups - around the protein to find binding hot spots ..
  56. Screening Assay for Human TAAR1 Ligands
    Brian P Gilmour; Fiscal Year: 2010
    ..of agonists and antagonists selective for this class of receptor will provide compounds useful as molecular probes of this function...
  57. Laser scanning cytometer for high throughput screening
    Caroline E Shamu; Fiscal Year: 2010
    ..Small molecule compounds thus identified can be powerful molecular probes for dissecting complex biological pathways, and may also be potential leads for development as therapeutics...
  58. Developing New Multicolor Polypeptide MR Contrast Agents for Stem Cell Tracking
    Michael T McMahon; Fiscal Year: 2010
    ..We are proposing developing a new class of molecular probes for use in cell tracking, inserting these into cells and monitoring their effectiveness in-vivo...
  59. Dissecting the biochemical role of BOK in regulating cellular life and death
    REBECCA I GOLDSTEIN; Fiscal Year: 2013
    ..SAHBs that directly bind to BOK will serve as molecular probes and prototype therapeutics to investigate and modulate the biological activity of BOK in vitro and in cells...
  60. Regulation of PD-1 as a strategy against chronic HIV-1 infection
    Li Huang; Fiscal Year: 2013
    ..Therefore, PD-1 is a valid drug target, and accomplishing the proposed studies will lay a foundation for further development of this class of small molecules as immunotherapeutic drug candidates for potential HIV-1 eradication. ..
  61. Imaging Vascular Phosphatidylethanolamine
    Ming Zhao; Fiscal Year: 2013
    ..Recently, we developed PE-specific molecular probes derived from Duramycin, which bind PE with high affinity and high specificity...
  62. Molecular Imaging of the Lung Using Hyperpolarized Carbon-13 Compounds
    Rahim R Rizi; Fiscal Year: 2013
    ..In addressing these hypotheses we propose the following specific aims: 1) Development and testing of molecular probes for studying glycolysis, amino acid synthesis, metabolism of inflammatory cells, and non-metabolitzed agents ..
  63. Beauveriolide-Derived Cyclodepsipeptides as a New Class of Anti-Alzheimer's Drugs
    Paul Wentworth; Fiscal Year: 2010
    ..of total protein production (BCA analysis) and free and esterified cholesterol (Amplex Red assay, Molecular Probes). Sub-aim 2.1 Measuring lipid loading in culture macrophages...
  64. REGULATION OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 1999
    ..Cholesterol 7alpha-hydroxylase cytochrome P450 (CYP7), specific antibodies and cDNA clones have been used as molecular probes to study molecular mechanisms of regulation...
  65. Novel Extended Red-Shifted Rhodamine Dyes
    NICHOLAS CAIRNS; Fiscal Year: 2004
    ..visible / near infra-red (NIR) region of the electromagnetic spectrum are excellent reporter components of molecular probes, fluorescent ligands, enzyme substrates, imaging contrast agents and general tracers...
  66. High-Throughput Identification of RNA polymerase inhibitors in vivo
    Robert Landick; Fiscal Year: 2006
    ..These lineage- specific inhibitors will provide powerful new molecular probes for investigating the biological function of RNA polymerase and valuable lead compounds to replenish the ..
  67. Novel Ovarian Cancer Detection Agents from Phage Display
    SUSAN DEUTSCHER; Fiscal Year: 2009
    ..New peptide-based molecular probes to facilitate cancer detection and imaging are rapidly evolving due to implementation of bacteriophage (phage)..
  68. MOLECULAR BIOLOGY OF COLLAGEN TYPES V AND VII
    DANIEL GREENSPAN; Fiscal Year: 2000
    ..In a more far-reaching study, molecular probes will be developed for PCR-based scanning for mutations in type V collagen DNA and RNA sequences of EDS ..
  69. Bivalent ligands as molecular probes for CB1/OX1 receptor heterodimers
    Yanan Zhang; Fiscal Year: 2010
    ..bivalent ligands, such as high molecular weight, are of concern, bivalent ligands have already been useful as molecular probes of heterodimer function in vivo...
  70. CORRELATIVE STUDIES USING SPECIMENS FROM SWOG 9321
    John Shaughnessy; Fiscal Year: 2000
    ..Triple-color interphase FISH analysis on 39 myeloma patients with a panel of molecular probes spanning the q arm of c13 has identified several deletion hotspots, 2 at 13q14 and 1 at 13q21...
  71. Magnetic Resonance Imaging of Alzheimer's Amyloid Plaque
    JOSEPH PODUSLO; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  72. Virtual True-Color Bronchoscopt to Detect Lung Cancer
    Geoffrey McLennan; Fiscal Year: 2004
    ..Such technology will be useful for the evaluation of molecular probes as they are developed, and in promoting image guided airway cancer treatment...
  73. DEVELOPMENT OF POTENTIALLY SELECTIVE DOPAMINE AGONISTS
    David E Nichols; Fiscal Year: 2010
    ..Novel ligands to be examined should provide molecular probes not only of the D1/D5 receptors, but also for all of the other dopamine receptor isoforms...
  74. Absorption and Metabolism of Oral Codeine in Mechanically Ventilated Neonates
    Jacob Aranda; Fiscal Year: 2009
    ....
  75. Signaling Proteins: Binding Studies and Peptide Mimetics
    Mark Spaller; Fiscal Year: 2006
    The goal of this Proposal is to design, prepare, and analyze a variety of ligands as molecular probes in the study of protein-protein interactions between macromolecules involved in signal transduction...