sulfhydryl reagents

Summary

Summary: Chemical agents that react with SH groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.

Top Publications

  1. pmc The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes
    Diana Nakunst
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, 33501 Bielefeld, Germany
    J Bacteriol 189:4696-707. 2007
  2. pmc Localization of the activation gate of a voltage-gated Ca2+ channel
    Cheng Xie
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Gen Physiol 126:205-12. 2005
  3. ncbi Transmembrane movement of the shaker K+ channel S4
    H P Larsson
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 16:387-97. 1996
  4. ncbi Reversible activation of secretory phospholipase A2 by sulfhydryl reagents
    Maiko Nabemoto
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    Arch Biochem Biophys 436:145-53. 2005
  5. ncbi Effect of sulfhydryl reagents on the regulatory system of the L-type Ca channel in frog ventricular myocytes
    K Yamaoka
    Department of Physiology, School of Medicine, Hiroshima University, Japan
    Pflugers Arch 440:207-15. 2000
  6. ncbi The mitochondrial tricarboxylate carrier of silver eel: chemical modification by sulfhydryl reagents
    Loredana Capobianco
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    J Biochem Mol Biol 37:515-21. 2004
  7. pmc Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: a reconsideration of the implications for insecticide design
    M Rowland
    Department of Biology, Birmingham Southern College, Birmingham, AL 35254, USA
    Chem Biol Interact 175:73-5. 2008
  8. pmc Changes in local S4 environment provide a voltage-sensing mechanism for mammalian hyperpolarization-activated HCN channels
    Damian C Bell
    Center for Neurobiology and Behavior, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    J Gen Physiol 123:5-19. 2004
  9. ncbi The interaction of the gamma-aminobutyric acid transporter GAT-1 with the neurotransmitter is selectively impaired by sulfhydryl modification of a conformationally sensitive cysteine residue engineered into extracellular loop IV
    Elia Zomot
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 278:42950-8. 2003
  10. ncbi Side chain orientation in the selectivity filter of a voltage-gated Ca2+ channel
    X S Wu
    Department of Pharmacology and Program in Neuroscience, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 275:31778-85. 2000

Research Grants

Detail Information

Publications232 found, 100 shown here

  1. pmc The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes
    Diana Nakunst
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, 33501 Bielefeld, Germany
    J Bacteriol 189:4696-707. 2007
    ..A typical SigH-responsive promoter was mapped upstream of the sigM gene. The ECF sigma factor SigM is apparently part of a regulatory cascade, and its transcription is controlled by SigH under conditions of thiol-oxidative stress...
  2. pmc Localization of the activation gate of a voltage-gated Ca2+ channel
    Cheng Xie
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Gen Physiol 126:205-12. 2005
    ..Our results suggest that the S6 helices of the alpha1 subunit of VGCCs undergo conformation changes during gating and the activation gate is located at the intracellular end of the pore...
  3. ncbi Transmembrane movement of the shaker K+ channel S4
    H P Larsson
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 16:387-97. 1996
    ..These results demonstrate that the putative voltage-sensing charges of S4 actually reside in the membrane and that they move outward when channels open. We consider constraints placed on channel structure by these results...
  4. ncbi Reversible activation of secretory phospholipase A2 by sulfhydryl reagents
    Maiko Nabemoto
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    Arch Biochem Biophys 436:145-53. 2005
    ..Both sulfhydryl reagents stimulated AA release from the HEK293T cells expressing human sPLA(2)X, and stimulated the sPLA(2) ..
  5. ncbi Effect of sulfhydryl reagents on the regulatory system of the L-type Ca channel in frog ventricular myocytes
    K Yamaoka
    Department of Physiology, School of Medicine, Hiroshima University, Japan
    Pflugers Arch 440:207-15. 2000
    ..It is concluded that there are sites on the Ca channels that are subject to direct modification by SH reagents...
  6. ncbi The mitochondrial tricarboxylate carrier of silver eel: chemical modification by sulfhydryl reagents
    Loredana Capobianco
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, via prov le Lecce Monteroni, I 73100 Lecce, Italy
    J Biochem Mol Biol 37:515-21. 2004
    ..Incubation of the proteoliposomes with various sulfhydryl reagents led to inhibition of the reconstituted citrate transport activity...
  7. pmc Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: a reconsideration of the implications for insecticide design
    M Rowland
    Department of Biology, Birmingham Southern College, Birmingham, AL 35254, USA
    Chem Biol Interact 175:73-5. 2008
    ..the inactivation of an invertebrate acetylcholinesterase, cholinesterase 2 from amphioxus, by the sulfhydryl reagents 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM)...
  8. pmc Changes in local S4 environment provide a voltage-sensing mechanism for mammalian hyperpolarization-activated HCN channels
    Damian C Bell
    Center for Neurobiology and Behavior, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA
    J Gen Physiol 123:5-19. 2004
    ....
  9. ncbi The interaction of the gamma-aminobutyric acid transporter GAT-1 with the neurotransmitter is selectively impaired by sulfhydryl modification of a conformationally sensitive cysteine residue engineered into extracellular loop IV
    Elia Zomot
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 278:42950-8. 2003
    ..accessible cysteine residue of the wild-type has been replaced by alanine, is also highly sensitive to the sulfhydryl reagents. Maximal sensitivity is observed in the presence of the cosubstrates sodium and chloride...
  10. ncbi Side chain orientation in the selectivity filter of a voltage-gated Ca2+ channel
    X S Wu
    Department of Pharmacology and Program in Neuroscience, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 275:31778-85. 2000
    ..Thus the structure of the Ca(2+) channel selectivity filter differs substantially from that of ancestral K(+) channels...
  11. ncbi Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme
    Leo Pezzementi
    Division of Science and Mathematics, Birmingham Southern College, Birmingham, AL 35222, USA
    Invert Neurosci 6:47-55. 2006
    ..to investigate the inactivation of an invertebrate acetylcholinesterase (AChE), ChE2 from amphioxus, by the sulfhydryl reagents 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM), creating various mutants, including ..
  12. ncbi Localization of the activation gate for small conductance Ca2+-activated K+ channels
    Andrew Bruening-Wright
    Vollum Institute, Oregon Health and Sciences University, Portland, Oregon 97201, USA
    J Neurosci 22:6499-506. 2002
    ..The results suggest that the SK channel gate is not formed by the cytoplasmic end of S6 but resides deep in the channel pore in or near the selectivity filter...
  13. ncbi Transporter-associated currents in the gamma-aminobutyric acid transporter GAT-1 are conditionally impaired by mutations of a conserved glycine residue
    Yonggang Zhou
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 280:20316-24. 2005
    ..Our results can be explained by a model invoking two outward-facing states of the empty transporter and a defective transition between these states in the glycine 80 mutants...
  14. pmc Functional architecture of the inner pore of a voltage-gated Ca2+ channel
    Xiao guang Zhen
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Gen Physiol 126:193-204. 2005
    ..This work provides an impetus for future studies on ion permeation, gating, and drug binding of VGCCs...
  15. pmc The glial and the neuronal glycine transporters differ in their reactivity to sulfhydryl reagents
    M J Roux
    Laboratoire de Neurobiologie, , Centre National de la Recherche Scientifique, , 46 rue d'Ulm, 75230 Paris Cedex 05, France
    J Biol Chem 276:17699-705. 2001
    ..Together, these results indicate that EL1 conformation differs between GlyT1b and GlyT2a and is modified by substrate binding and translocation...
  16. pmc KCNE1 and KCNE3 stabilize and/or slow voltage sensing S4 segment of KCNQ1 channel
    Koichi Nakajo
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, 444 8585, Japan
    J Gen Physiol 130:269-81. 2007
    ..These results offer new insight into the mechanism of KCNQ1 channel modulation by KCNE1 and KCNE3...
  17. ncbi Cytoplasmic vestibule of the weak inward rectifier Kir6.2 potassium channel
    Yijun Cui
    Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Biol Chem 277:10523-30. 2002
    ..Data are consistent with the hypothesis that residues Ile-210-Ser-212 line a funnel-shaped vestibule of 20-25 A in diameter, which remains unchanged during channel gating...
  18. ncbi Resolution, detection, and characterization of redox conformers of human HSF1
    D J Manalo
    Graduate Program in Cell and Developmental Biology and Department of Cell Biology and Neuroscience, Rutgers State University, Piscataway, New Jersey 08854-8082, USA
    J Biol Chem 276:23554-61. 2001
    ..Our result suggests that hHSF1 may have integrated redox chemistry of cysteine sulfhydryl into its functional responses...
  19. ncbi Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates
    K Sridevi
    National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bangalore, India 560065
    Biochemistry 41:1568-78. 2002
    ....
  20. ncbi Reduced redox state allows prolonged survival of axotomized neonatal retinal ganglion cells
    L K Geiger
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 600 Highland Avenue, Madison, WI 53792, USA
    Neuroscience 109:635-42. 2002
    ..Understanding the mechanisms by which ROS signal neuronal death could result in strategies for increasing their long-term survival after axonal injury...
  21. ncbi Evaluation of critical structural elements of UDP-sugar substrates and certain cysteine residues of a vertebrate hyaluronan synthase
    Philip E Pummill
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:21610-6. 2002
    ..Mutation of Cys337 raises the UDP-GlcUA Michaelis constant (K(m)), suggesting that this residue participates in UDP-GlcUA substrate binding or in catalytic complex formation...
  22. ncbi Localization of disulfide bonds in the cystine knot domain of human von Willebrand factor
    A Katsumi
    Howard Hughes Medical Institute, Department of Medicine and Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 275:25585-94. 2000
    ....
  23. ncbi Mutational analysis of the conserved cysteines of the rat P2X2 purinoceptor
    J Dylan Clyne
    Department of Molecular, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Neurosci 22:3873-80. 2002
    ..We also suggest that C124, C130, C147, and C158 form two disulfide bonds, but we are unable to assign specific cysteine pairs to these two bonds...
  24. ncbi A possible effect of sulfhydryl reagents on the contractile activity of the rat detrusor muscle
    Sefa Resim
    Department of Urology, Medical Faculty, Sutcu Imam University, Kahramanmaras, Turkey
    Eur J Pharmacol 442:295-9. 2002
    ..Further experiments are needed to determine the cellular mechanism(s) of action by which these SH reagents act on the detrusor smooth muscle...
  25. ncbi Site-directed mutagenesis and chemical modification of the six native cysteine residues of the rat mitochondrial carnitine carrier: implications for the role of cysteine-136
    Cesare Indiveri
    Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, 70125 Bari, Italy
    Biochemistry 41:8649-56. 2002
    ..A model of the CAC is proposed in which the matrix hydrophilic loop containing Cys-136 protrudes into the membrane between the transmembrane domains of the protein...
  26. ncbi Modulation of ICl(Ca) in vascular smooth muscle cells by oxidizing and cysteine-reactive reagents
    Iain A Greenwood
    Department of Pharmacology and Clinical Pharmacology, Cardiovascular Research Group, St Georges Hospital Medical School, London, UK
    Pflugers Arch 443:473-82. 2002
    ..Similar effects were observed with the hydrophilic thiol-reactants thimerosal and p-chloromercuriphenylsulphonic acid (PCMPS). Therefore, the gating and activation of Ca2+-activated Cl- conductance is sensitive to redox modification...
  27. ncbi The presence of an aqueous cavity in the proton-pumping pathway of the pyridine nucleotide transhydrogenase of Escherichia coli is suggested by the reaction of the enzyme with sulfhydryl inhibitors
    P D Bragg
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, V6T 1Z3, Canada
    Arch Biochem Biophys 380:141-50. 2000
    ..A further series of mutants of betaGlu124 on helix 10 confirms the proposal (P. D. Bragg and C. Hou, Arch. Biochem. Biophys. 363, 182-190, 1999) that this residue is involved in passive permeation of protons across domain II...
  28. ncbi The effect of modifications of the charged residues in the transmembrane helices on the transport activity of the melibiose carrier of Escherichia coli
    P Z Ding
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Biochem Biophys Res Commun 285:348-54. 2001
    ..we describe experiments in which the cysteine substitution for a charged residue was chemically changed by sulfhydryl reagents (MTSEA and MTSET to restore a positive charge and MTSES a negative charge) or by iodoacetic acid or through ..
  29. pmc Exploration of the pore structure of a peptide-gated Na+ channel
    M Poet
    Laboratoire de Physiologie Cellulaire et Moleculaire, CNRS UMR6548, Universite de Nice Sophia Antipolis, Faculte des Sciences, Parc Valrose, 06108 Nice, France
    EMBO J 20:5595-602. 2001
    ..Summarizing, the ionic pore of FaNaC includes a large aqueous cavity, with a charge selectivity filter in the outer vestibule and the gate close to the interior...
  30. ncbi Three pairs of cysteine residues mediate both redox and zn2+ modulation of the nmda receptor
    Y Choi
    Center for Neuroscience and Aging, The Burnham Institute, La Jolla, California 92037, USA
    J Neurosci 21:392-400. 2001
    ..Thus, these cysteine residues do not appear to coordinate Zn(2+) directly. Instead, the redox status of these cysteine residues may modulate the sensitivity of the receptor to Zn(2+)...
  31. ncbi Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implications
    R Rossi
    Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
    J Biol Chem 276:7004-10. 2001
    ..This is thought to influence significantly the biochemical, pharmacological, and toxicological effects of some drugs...
  32. ncbi The role of cysteine residues in tellurite resistance mediated by the TehAB determinant
    M Dyllick-Brenzinger
    Structural Biology Research Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
    Biochem Biophys Res Commun 277:394-400. 2000
    ..Studies using native and SDS denaturing PAGE under reducing and oxidizing conditions suggested that a cysteine in TehB is involved in binding tellurite...
  33. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
    ..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
  34. ncbi Single-molecule covalent chemistry with spatially separated reactants
    Tudor Luchian
    Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843 1114, USA
    Angew Chem Int Ed Engl 42:3766-71. 2003
  35. ncbi The streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function
    Kshama Kumari
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 277:13943-51. 2002
    ..Both seHAS and spHAS were inhibited by sulfhydryl reagents such as N-ethylmaleimide (NEM) and iodoacetamide in a dose-dependent and time-dependent manner...
  36. pmc The influence of oxidation of membrane thiol groups on lysosomal proton permeability
    F Y Wan
    Department of Cellular Biophysics, Institute of Biophysics, Academia Sinica, Beijing, 100101, People's Republic of China
    Biochem J 360:355-62. 2001
    ..The results raise the possibility that the proton permeability of lysosomes in vivo may be affected by the redox states of their membrane thiol groups...
  37. ncbi Comparative surface accessibility of a pore-lining threonine residue (T6') in the glycine and GABA(A) receptors
    Qiang Shan
    Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 277:44845-53. 2002
    ..The results also indicate that the GABA(A)R pore structure at the 6' level may vary between different expression systems...
  38. ncbi Accessibility and conformational coupling in serotonin transporter predicted internal domains
    Andreas Androutsellis-Theotokis
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520 8066, USA
    J Neurosci 22:8370-8. 2002
    ..The reactivity of many of the cytoplasmic cysteine residues studied was influenced by ion and ligand binding, suggesting that the internal domains of SERT participate in conformational changes during neurotransmitter transport...
  39. ncbi S-nitrosylation of N-ethylmaleimide sensitive factor mediates surface expression of AMPA receptors
    Yunfei Huang
    Department of Neuroscience, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Neuron 46:533-40. 2005
    ....
  40. ncbi Cysteine modification by lipid peroxidation products inhibits protein disulfide isomerase
    David L Carbone
    Department of Pharmaceutical Sciences, School of Pharmacy, The University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Chem Res Toxicol 18:1324-31. 2005
    ..The data presented here thus suggest potential impairment of an important molecular chaperone during oxidative stress...
  41. ncbi Structural and functional characterization of transmembrane segment IX of the NHE1 isoform of the Na+/H+ exchanger
    Tyler Reddy
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada
    J Biol Chem 283:22018-30. 2008
    ..The results suggest that transmembrane IX is critical with pore-lining residues and a kink at the functionally important residue Ser(351)...
  42. ncbi Proteomics approaches to study the redox state of cysteine-containing proteins
    Serena Camerini
    Unita di Spettrometria di Massa, DIBIT, Istituto Scientifico San Raffaele, Milan, Italy
    Ann Ist Super Sanita 41:451-7. 2005
    ..This methodology can be used in proteomic studies to recognize redox-sensitive Cysteine containing proteins and nitric oxide targets...
  43. ncbi Reducing and oxidizing agents sensitize heat-activated vanilloid receptor (TRPV1) current
    Klara Susankova
    Department of Cellular Neurophysiology, Institute of Physiology AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mol Pharmacol 70:383-94. 2006
    ..The results obtained demonstrate that an optimal redox state is crucial for the proper functioning of the TRPV1 channel and both its reduced and oxidized states can result in an increase in responsiveness to thermal stimuli...
  44. ncbi Fatty acyl-CoA as an endogenous activator of UDP-glucuronosyltransferases
    Kazuharu Okamura
    Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 345:1649-56. 2006
    ..These results suggest that: (1) acyl-CoAs play a role as an endogenous activator of UGTs, and (2) a sulfhydryl group is required for the activation of UGT by physiological concentrations of acyl-CoAs...
  45. ncbi Transcriptional activation of dehalorespiration. Identification of redox-active cysteines regulating dimerization and DNA binding
    Stelian M Pop
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 281:26382-90. 2006
    ..Therefore, reversible redox inactivation is manifested at the level of DNA binding. Our studies reveal a strategy for limiting expression of a redox-sensitive pathway by using a thiol-based redox switch in the transcription factor...
  46. ncbi Conformationally sensitive reactivity to permeant sulfhydryl reagents of cysteine residues engineered into helical hairpin 1 of the glutamate transporter GLT-1
    Irina Shlaifer
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel
    Mol Pharmacol 71:1341-8. 2007
    ..According to this model, the inward movement of hairpin 1 results in the opening of a pathway between the binding pocket and the cytoplasm, lined by parts of transmembrane domains 7 and 8...
  47. ncbi Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1
    Suhong Li
    Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
    Biosci Biotechnol Biochem 71:449-57. 2007
    ....
  48. ncbi Structural insights into the role of the ACTH receptor cysteine residues on receptor function
    Yingkui Yang
    Division of Pediatric Surgery, University of Alabama at Birmingham, 1600 7th Avenue South, Birmingham, AL 35233, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1120-6. 2007
    ..Further experiments suggest that a disulfide bond exists between the residue C245 and C251 in EL3. These findings provide important insights into the importance of cysteine residues of hMC2R for receptor function...
  49. pmc An intersubunit zinc binding site in rat P2X2 receptors
    Naomi Nagaya
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 280:25982-93. 2005
    ..Taken together, these data indicate that His120 and His213 sit close to each other across the interface between subunits and are likely to be key components of the zinc binding site in P2X2 receptors...
  50. ncbi Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistry
    Go Hasegawa
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    J Biochem Biophys Methods 63:33-42. 2005
    ..These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins...
  51. ncbi The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane
    Elisa B Vervoort
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Mol Biol 346:733-43. 2005
    ..These and other results suggest that the predicted loop between TM2 and TM3 may fold back into the membrane and form part of the translocation path...
  52. ncbi Detection of oxidant sensitive thiol proteins by fluorescence labeling and two-dimensional electrophoresis
    James W Baty
    Free Radical Research Group, Department of Pathology, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand
    Proteomics 2:1261-6. 2002
    ..Separation of proteins by 2-D electrophoresis revealed approximately 200 thiol proteins that were oxidized by diamide treatment. This method will be valuable in elucidating redox signaling pathways...
  53. pmc Heterogeneity in the structural dynamics of Sulfolobus solfataricus beta-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry
    Evgenia Lozinsky
    Department of Chemistry, Ben Gurion University of the Negev, 84105 Beer Sheva, Israel
    Protein Sci 11:2535-44. 2002
    ..e., 328 K and 338 K, respectively). The Arrhenius analysis of data provided the activation energy relative to the conformational changes characterizing the local and global movements running through the protein matrix...
  54. ncbi 20 S proteasome from Saccharomyces cerevisiae is responsive to redox modifications and is S-glutathionylated
    Marilene Demasi
    Departmento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Rua do Matao, 277 São Paulo, Sao Paulo 05508 900, Brazil
    J Biol Chem 278:679-85. 2003
    ..The present results indicate that at the physiological level the yeast 20 S proteasome is regulated by its sulfhydryl content, thereby coupling intracellular redox signaling to proteasome-mediated proteolysis...
  55. ncbi Coupling of contact sensitizers to thiol groups is a key event for the activation of monocytes and monocyte-derived dendritic cells
    Detlef Becker
    Department of Dermatology, University of Mainz, Germany
    J Invest Dermatol 120:233-8. 2003
    ..Our data show that strong affinity of a small reactive chemical toward thiol groups is important for the activation of monocytes and monocyte-derived dendritic cells and can support the process of sensitization...
  56. pmc Structure-function analysis of the bestrophin family of anion channels
    Takashi Tsunenari
    Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 278:41114-25. 2003
    ..These experiments provide the first structural analysis of the bestrophin channel family...
  57. pmc Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimers
    Philip J Budge
    Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Antimicrob Agents Chemother 47:3470-7. 2003
    ....
  58. ncbi Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents
    T Belyaeva
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cell Biol Int 27:887-95. 2003
    ..Thus, both the experimental observations obtained in this study and the literature data imply a significant role of redox-based mechanisms in regulating lysosomal functional activity...
  59. ncbi Increased rigidity of domain structures enhances the stability of a mutant enzyme created by directed evolution
    Jun Hoseki
    Department of Biochemistry, Osaka Medical College, Takatsuki, Japan
    Biochemistry 42:14469-75. 2003
    ..The present results suggest that the 19 mutations of HTK stabilize KNT by affecting the dynamic behavior of the structure of this enzyme without significantly changing its static overall structure...
  60. ncbi Preparation and utilization of a reagent for the isolation and purification of low-molecular-mass thiols
    Daniel J Steenkamp
    Division of Chemical Pathology, Faculty of Health Sciences, University of Cape Town, Observatory 7935, Cape Town, South Africa
    Anal Biochem 325:21-7. 2004
    ..Following their isolation as the heterodisulfides the thiol species of interest are recovered by reduction and facile separation from the apolar 2-mercapto-6-hydroxynaphthalene partner...
  61. ncbi Cysteine-scanning mutagenesis of transmembrane segment 1 of glucose transporter GLUT1: extracellular accessibility of helix positions
    Matthias Heinze
    Institute of Pharmacology, Freie Universitat Berlin, Thielallee 67 73, D 14195 Berlin, FRG
    Biochemistry 43:931-6. 2004
    ..a membrane-permeable agent, was used to identify positions that are sensitive to transport alteration by sulfhydryl reagents, whereas uptake modification by the membrane-impermeant pCMBS indicated accessibility to water-soluble ..
  62. pmc Subunit a facilitates aqueous access to a membrane-embedded region of subunit c in Escherichia coli F1F0 ATP synthase
    P Ryan Steed
    Department of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 283:12365-72. 2008
    ..The results described here provide further evidence for an aqueous-accessible region at the interface of subunits a and c extending from the middle of the membrane to the cytoplasm...
  63. ncbi Molecular characterization of the hdm2-p53 interaction
    A Bottger
    Cancer Research Campaign Laboratories, University of Dundee, Scotland, UK
    J Mol Biol 269:744-56. 1997
    ..All our results are discussed in the light of the recently published crystal structure of the mdm2-p53 complex. A striking correspondence between our findings and the crystal structure is revealed...
  64. ncbi Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid)
    M J Nozal
    Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain
    J Chromatogr A 778:347-53. 1997
    ..The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively...
  65. ncbi Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli
    M Yamaguchi
    J Biochem 86:325-33. 1979
    ..Similarly, other sulfhydryl reagents such as p-hydroxymercuribenzoate (PMB), 5,5'-dithiobis(2-nitrobenzoate) (DTNB), and iodoacetate inhibited ..
  66. ncbi Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W
    M Iwakura
    J Biochem 86:1239-49. 1979
    ..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme...
  67. ncbi Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes
    G Gao
    Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
    J Biol Chem 269:8234-9. 1994
    ..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
  68. ncbi Structural and functional determinants in the S5-P region of HCN-encoded pacemaker channels revealed by cysteine-scanning substitutions
    Ka Wing Au
    Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong
    Am J Physiol Cell Physiol 294:C136-44. 2008
    ..Based on these results, we conclude that pore residues 339-345 are important determinants of the structure-function properties of HCN channels and that the side chain of H344 is externally accessible...
  69. ncbi Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coli
    S R Dowd
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1252:278-83. 1995
    ..Cysteine 492 has been identified as containing the sulfhydryl that is accessible to the reagent under both conditions...
  70. ncbi Site-directed mutagenesis of the Cys residues in ClpA, the ATPase component of protease Ti (ClpAP) in Escherichia coli
    J H Seol
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea
    Biol Chem 378:1205-9. 1997
    ..These results suggest that the inhibitory effect of NEM is due to prevention of the interaction of ClpA with casein by introduction of a bulky alkyl group to Cys47, but not linked to the catalytic function of the ATPase...
  71. ncbi Oxalacetate decarboxylase and pyruvate carboxylase activities, and effect of sulfhydryl reagents in malic enzyme from Sulfolobus solfataricus
    A Guagliardi
    Dipartimento di Chimica Organica e Biologica, Universita di Napoli, Italia
    Biochim Biophys Acta 957:301-11. 1988
    ..The inactivation of the oxaloacetate decarboxylase activity by p-hydroxymercuribenzoate treatment was found to occur at a slower rate than that of the oxidative decarboxylase activity...
  72. ncbi Sequence comparison between the flavoprotein subunit of the fumarate reductase (complex II) of the anaerobic parasitic nematode, Ascaris suum and the succinate dehydrogenase of the aerobic, free-living nematode, Caenorhabditis elegans
    T Kuramochi
    Department of Parasitology, University of Tokyo, Japan
    Mol Biochem Parasitol 68:177-87. 1994
    ..The sensitivity of other Fp subunits to sulfhydryl reagents appears to reside in a cysteine immediately preceding a conserved arginine in the putative active site...
  73. ncbi Activation of a NADH dehydrogenase in the human erythrocyte by beta-adrenergic agonists: possible involvement of a G protein in enzyme activation
    F Marques
    Central Analysis Laboratory, Aveiro University, Portugal
    Biol Signals 6:52-61. 1997
    ..Basal and agonist-dependent activities were inhibited in the presence of sulfhydryl reagents p-chloromercuribenzoate (PCMB, 10(-5) M) and N-ethylmaleimide (NEM, 10(-3) M), indicating the involvement ..
  74. ncbi Purification and characterization of N-acetylglucosamine kinase from rat liver--comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
    S Hinderlich
    Institut fur Molekularbiologie und Biochemie, Freie Universitat Berlin, Germany
    Eur J Biochem 252:133-9. 1998
    ..that the substrates GlcNAc and ManNAc protected their respective enzymes from inhibition by the above sulfhydryl reagents indicates that Cys residues are located in or near the active sites of both enzymes...
  75. ncbi Identification and characterization of the enzymatic activity of zeta-crystallin from guinea pig lens. A novel NADPH:quinone oxidoreductase
    P V Rao
    Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 267:96-102. 1992
    ..Activity was sensitive to heat and sulfhydryl reagents but was very stable on freezing. Dicumarol (Ki = 1.3 x 10(-5) M) and nitrofurantoin (Ki = 1...
  76. ncbi Studies on structural stability of thermophilic Sulfolobus acidocaldarius ribosomes
    Kalavathi Yangala
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    Indian J Biochem Biophys 44:19-25. 2007
    ..Thermophilic ribosomes treated with destabilizing agents, such as sulfhydryl reagents [5,5'-Dithio-bis-(2-nitrobenzoic acid), N-ethylmaleimide and p-hydroxymercurybenzoate) and intercalating ..
  77. ncbi Sulfhydryl reagent susceptibility in proteins with high sequence similarity--triosephosphate isomerase from Trypanosoma brucei, Trypanosoma cruzi and Leishmania mexicana
    G Garza-Ramos
    Departamento de Bioquímica Instituto de Fisiología Celular, Universidad Nacional Autonoma de Mexico, Mexico, DF
    Eur J Biochem 253:684-91. 1998
    ..Thus, sulfhydryl reagents act on Cys14...
  78. ncbi Branched chain aminotransferase isoenzymes. Purification and characterization of the rat brain isoenzyme
    T R Hall
    Department of Biochemistry, Wake Forest University, Bowman Gray School of Medicine, Winston Salem, North Carolina 27157
    J Biol Chem 268:3092-8. 1993
    ..Experiments with protein labeling reagents, in particular sulfhydryl reagents, also suggested that there may be some distinct structural differences in BCATc and BCATm...
  79. ncbi The complete amino acid sequence of the low molecular weight cytosolic acid phosphatase
    G Camici
    Department of Biochemical Sciences, University of Florence, Italy
    J Biol Chem 264:2560-7. 1989
    ..family is located in the cytosol, is not inhibited by L-(+)-tartrate and fluoride ions, but is inhibited by sulfhydryl reagents. The enzyme consists of 157 amino acid residues, has an acetylated NH2 terminus, and has arginine as the ..
  80. ncbi [Tartrate-sensitive and tartrate-resistant acid phosphatases in Amoeba proteus]
    V A Sopina
    Institute of Cytology RAS, St Petersburg
    Tsitologiia 42:602-12. 2000
    ..On the contrary, it appeared to be resistant to sulfhydryl reagents--4(hydroxymercury) benzoic acid, sodium salt and N-ethylmaleimide...
  81. pmc Molecular characterization of a germination-specific muramidase from Clostridium perfringens S40 spores and nucleotide sequence of the corresponding gene
    Y Chen
    Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Aichi, Japan
    J Bacteriol 179:3181-7. 1997
    ..The enzyme was inactivated by sulfhydryl reagents, and sodium thioglycolate reversed the inactivation by Hg2+...
  82. ncbi Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II)
    Jill K Paterson
    Department of Biochemistry and Molecular Biology, Medical Sciences, Indiana University, Bloomington, Indiana 47405, USA
    Biochemistry 45:5367-76. 2006
    ..PS to the cytosolic leaflet of the bilayer and is sensitive to vanadate, Ca(2+), and modification by sulfhydryl reagents. Although the flippase has not been positively identified, a subfamily of P-type ATPases has been proposed ..
  83. pmc Purification and characterization of cysteine protease from germinating cotyledons of horse gram
    Rajeswari Jinka
    Center for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India
    BMC Biochem 10:28. 2009
    ..To study the mechanism of protein mobilization, undertaken the task of purifying and characterizing proteases, which occur transiently in germinating seeds of horse gram...
  84. ncbi Reconstitution into liposomes of the B degrees -like glutamine-neutral amino acid transporter from renal cell plasma membrane
    Francesca Oppedisano
    Department of Cell Biology, University of Calabria, Via P Bucci 4c, 87036 Arcavacata di Rende, Italy
    Biochim Biophys Acta 1778:2258-65. 2008
    ..The sulfhydryl reagents HgCl2, mersalyl, p-hydroxymercuribenzoate and the substrate analogue 2-aminobicyclo[2,2,1]heptane-2-..
  85. ncbi Identification of the mitochondrial branched chain aminotransferase as a branched chain alpha-keto acid transport protein
    S M Hutson
    Department of Biochemistry, Bowman Gray School of Medicine, Winston Salem, North Carolina 27157 1016
    J Biol Chem 268:3084-91. 1993
    ..Studies with covalent protein-modifying reagents indicated that transport was inhibited by sulfhydryl reagents, the histidine reagent diethyl pyrocarbonate, and the tyrosine reagent N-acetylimidazole...
  86. pmc Transmembrane domain 8 of the {gamma}-aminobutyric acid transporter GAT-1 lines a cytoplasmic accessibility pathway into its binding pocket
    Assaf Ben-Yona
    Department of Biochemistry, Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 284:9727-32. 2009
    ..Cysteine-399 is the major site of inhibition of GAT-1 by membrane-permeant sulfhydryl reagents. This cysteine residue was previously thought to reside on a cytoplasmic loop connecting transmembrane ..
  87. ncbi A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae
    E N Elpidina
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Leninskie Gory, Moscow 119992, Russia
    Biochimie 87:771-9. 2005
    ..However, the activity of TmC1 was reduced with sulfhydryl reagents. Several plant and insect proteinaceous proteinase inhibitors were active against the purified enzyme, the ..
  88. ncbi Effects of cysteine modification on the activity of the cGMP-gated channel from retinal rods
    V Serre
    C E A, URA CNRS n 520, Departement de Biologie Moleculaire et Structurale, Centre d Etudes Nucleaires de Grenoble, France
    J Membr Biol 146:145-62. 1995
    The effect of sulfhydryl reagents on the activity of the cGMP-gated channel from bovine retinal rods was studied by measurements of 8-Br-cGMP-(cGMP)-induced calcium efflux from rod membrane vesicles and records of 8-Br-cGMP-dependent ..
  89. ncbi Kinetic analysis of the slow ionization of glutathione by microsomal glutathione transferase MGST1
    R Morgenstern
    Institute of Environmental Medicine, Division of Biochemical Toxicology, Karolinska Institutet, Box 210, S 171 77 Stockholm, Sweden
    Biochemistry 40:3378-84. 2001
    ..4). Microsomal glutathione transferase 1 displays the unusual property of being activated by sulfhydryl reagents. When the enzyme is activated by N-ethylmaleimide, the rate of thiolate anion formation is greatly enhanced,..
  90. ncbi Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties
    R A Nicholas
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    J Biol Chem 263:2034-40. 1988
    ..Alkylation of cysteine 115 with sulfhydryl reagents has previously been shown to inhibit severely the D-alanine carboxypeptidase activity of PBP 5...
  91. ncbi Effect of membrane-permeable sulfhydryl reagents and depletion of glutathione on calcium mobilisation in human platelets
    R M van Gorp
    Department of Human Biology, Maastricht University, The Netherlands
    Biochem Pharmacol 53:1533-42. 1997
    ..Similar platelet-activating effects are induced by sulfhydryl reagents that evoke Ca2+-induced Ca2+ release (CICR) by stimulating the Ca2+-releasing property of the inositol-1,4,..
  92. pmc Isolation and characterization of the DNA-binding protein (DBP) of the Autographa californica multiple nucleopolyhedrovirus
    Victor S Mikhailov
    Department of Microbiology, Oregon State University, Corvallis, OR 97331 3804, USA
    Virology 370:415-29. 2008
    ..The unwinding and renaturation activities of DBP, as well as the DNA binding activity, were sensitive to sulfhydryl reagents and were inhibited by oxidation of thiol groups with diamide or by alkylation with N-ethylmaleimide...
  93. ncbi Purification and characterization of protein carboxyl methyltransferase from Torpedo ocellata electric organ
    R Haklai
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
    Biochemistry 26:4200-6. 1987
    ..Purified Torpedo PCM is highly sensitive to sulfhydryl reagents. The competitive inhibitor of PCM S-adenosyl-L-homocysteine (AdoHcy) protected the enzyme from inactivation ..
  94. ncbi Regulation of chicken gizzard ecto-ATPase activity by modulators that affect its oligomerization status
    C C Caldwell
    Department of Chemistry, San Diego State University, California 92182 1030, USA
    Arch Biochem Biophys 387:107-16. 2001
    ..Compounds that inhibit some ion-motive ATPases, e.g., sulfhydryl reagents, xanthene derivatives, NBD-halides, and suramin, also inhibit the gizzard ecto-ATPase, but not another E-..
  95. ncbi Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis
    A Hagting
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Haren, The Netherlands
    Biochemistry 36:6777-85. 1997
    ..of single cysteine mutants in putative cytoplasmic or extracellular loops by membrane (im)permeant sulfhydryl reagents. The deduced topology model of DtpT consists of a bundle of 12 alpha-helixes with a short amino and a large ..
  96. ncbi The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase
    M A Birch-Machin
    Division of Clinical Neuroscience, University of Newcastle upon Tyne, Great Britain
    J Biol Chem 267:11553-8. 1992
    ..The active site of the bovine polypeptide contains a cysteine that confers sensitivity of the enzyme to sulfhydryl reagents; this cysteine is only present in some sequences and thus provides a discriminatory biochemical marker...
  97. ncbi Interaction of two sulfhydryl reagents with a cation recognition site on the neuronal dopamine carrier evidences small differences between [3H]GBR 12783 and [3H]cocaine binding sites
    F Refahi-Lyamani
    EP 076 du C N R S, U F R de Médecine and Pharmacie de Rouen, Saint Etienne du Rouvray, France
    Naunyn Schmiedebergs Arch Pharmacol 351:136-45. 1995
    We have compared the effect of treating rat striatal cell membranes with ionic hydrophilic sulfhydryl reagents on the specific bindings of [3H]cocaine and of [3H]GBR 12783 (1-[2-(diphenylmethoxy)ethyl]4-(3-phenyl-2-[1-3H]propenyl)-..
  98. ncbi Amino acids defining the acyl pocket of an invertebrate cholinesterase
    Leo Pezzementi
    Division of Science and Mathematics, Birmingham Southern College, Box 549022, Birmingham, AL 35254, USA
    Comp Biochem Physiol B Biochem Mol Biol 136:813-32. 2003
    ..Our results also provide information about the aromatic trapping of the active site histidine and the inactivation of ChE2 by sulfhydryl reagents.
  99. ncbi Characterization and reconstitution of a cell free system for NAD(+)-dependent deoxyhypusine formation on the 18 kDa eIF-4D precursor
    Q P Dou
    Department of Chemistry, Rutgers University, New Brunswick, NJ
    Biochim Biophys Acta 1036:128-37. 1990
    ..was sensitive to buffer composition; buffers made of basic amino acids and Tris were inhibitory, (iii) sulfhydryl reagents and metal ions such as Cu2+ and Fe3+ were potent inhibitors of deoxyhypusine formation and (iv) the 18 kDa ..
  100. ncbi Flow cytometric detection of transbilayer movement of fluorescent phospholipid analogues across the boar sperm plasma membrane: elimination of labeling artifacts
    B M Gadella
    Graduate School of Animal Health, Department of Herd Health and Animal Reproduction, Faculty of Veterinary Sciences, Utrecht University, The Netherlands
    Mol Reprod Dev 53:108-25. 1999
    ..These inward movements of fluorescent phospholipids were ATP-dependent and could be blocked with sulfhydryl reagents. Movements from the inner to the outer leaflet of the sperm plasma membrane were minimal for intact ..
  101. ncbi APMA (4-aminophenylmercuric acetate) activation of stromelysin-1 involves protein interactions in addition to those with cysteine-75 in the propeptide
    G Galazka
    Research Center in Oral Biology, University of Alabama at Birmingham 35294, USA
    Biochemistry 35:11221-7. 1996
    ..10289-10295] demonstrated that modification of this cysteine residue in the propeptide of stromelysin-1 by sulfhydryl reagents did not result in an active enzyme as predicted...

Research Grants50

  1. Linking functions of the FOF1 ATP Synthase
    Robert Nakamoto; Fiscal Year: 2005
    ..and determination of solvent accessibility of cysteine substitutions by reactivity with hydrophilic sulfhydryl reagents will be used to generate a structure-function map of the gamma and epsilon rotor subunits...
  2. CONTRIBUTION OF COLLAGEN TO VENTRICULAR STIFFNESS
    JAMES CAULFIELD; Fiscal Year: 1990
    ..We have determined that oxidized sulfhydryl reagents can induce a collagenolytic activity in these cardiac preparations...
  3. BIOCHEMICAL RESEARCH ON OPIOID RECEPTORS
    Eric Simon; Fiscal Year: 2003
    ..of other modifications such as palmitoylation of C- terminal cysteines and alkylation of cysteines by sulfhydryl reagents. The protein, present in earlier preparations, that gave rise to peptides not found in the sequence of the ..
  4. (2'-5')AN DEPENDENT ENDONUCLEASE AND INTERFERON ACTION
    GEORGIA SMITH; Fiscal Year: 1992
    ..with regard to its activation by (2'- 5')oligoadenylates, its substrate specificity, and its sensitivity to sulfhydryl reagents. Antibody to RNase L will be produced and used to facilitate purification and functional characterization...
  5. STRUCTURE-FUNCTION STUDIES OF A SWITCHABLE ION CHANNEL
    FRANCOISE LEVINTHAL; Fiscal Year: 1991
    ..The effects of these sulfhydryl reagents will be examined by either electron- spin resonance spectroscopy if the attached molecule's have an ..
  6. FACTORS MODIFYING THE REACTION PROPERTIES OF HB S
    John Taylor; Fiscal Year: 1980
    ..g., Phe-Phe-Arg), sugars, aldehydes, acetylating agents (e.g., aspirins) and sulfhydryl reagents (e.g., cystamine)...
  7. MORPHOLOGICAL STUDIES ON MECHANISM OF INSULIN ACTION
    Leonard Jarett; Fiscal Year: 1980
    ..reanalysis of ferritin-insulin distribution and the effect of temperature, bifunctional crosslinkers and sulfhydryl reagents on ferritin-insulin distribution...
  8. MECHANISM INFLUENCING LIGAND-GATED CA2+ RELEASE CHANNELS
    Isaac Pessah; Fiscal Year: 1993
    ..Fluorescent sulfhydryl reagents clarify the role and position of this domain within the native LGCRC oligomer and discriminate major ..
  9. NITROGENOUS LIGANDS AND HEPATIC MONOOXYGENASES
    HOWARD SHERTZER; Fiscal Year: 1980
    ..d) affect the mixed-function oxidase activity using these substrates; and e) influence the effects of sulfhydryl reagents, sulfhydryl inhibitors, and free radical scavengers on enzyme activity and substrate binding...
  10. Conformational Changes Leading to Rhodopsin Activation
    H Khorana; Fiscal Year: 2007
    ..every one of the amino acids in the cytoplasmic and TM domains will be prepared and their reactivities to sulfhydryl reagents will be compared in the dark and after illumination...
  11. DOPAMINE TRANSPORTER AND COCAINE
    Maarten Reith; Fiscal Year: 2003
    ..cell surface expression (biotinylation), DAT function (DA uptake and blocker binding), sensitivity towards sulfhydryl reagents (membrane permeable and impermeable), and their capability to form dimers (co-transfection of wild-type and ..
  12. AFM Of Voltage And Mechanically Gated Channels
    Frederick Sachs; Fiscal Year: 2004
    ..will be connected to cloned, cysteine mutant, K+ channels that with linkers bearing maleimide or other sulfhydryl reagents. Using gating current protocols, experiments will measure the distance that different sites in the channel ..
  13. MECHANISM OF RENAL ORGANIC ANION TRANSPORT
    BRENDA CHAN; Fiscal Year: 2000
    ..cloned mouse PAH transporter (mPAHT) 2) Determine transmembrane topology of mPAHT using biotin containing sulfhydryl reagents 3) Characterize the substrate binding site and substrate translocation pathway of mPAHT 4) Identify ..
  14. Neuromodulation of Voltage-gated Ca2+ Channels
    Jian Yang; Fiscal Year: 2007
    ..This research may not only enhance our understanding of the complex regulatory pathways for VGCCs but also provide valuable information for the development of potential therapeutic reagents that target these pathways ..
  15. Acetylcholinesterase: A Comparative Biochemical Approach
    Leo Pezzementi; Fiscal Year: 2005
    ..abstract_text> ..
  16. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2009
    ..Understanding the role and mechanism of axon atrophy in solvent neurotoxicity has broad-based implications for human occupational health and risk assessment. ..
  17. MOLECULAR PHYSIOLOGY OF INWARDLY RECTIFYING K CHANNELS
    Jian Yang; Fiscal Year: 2001
    ..They will also allow us to gain better insight into the molecular architecture of the channel pore, which may help in the development of pharmacological agents directed at these channels. ..
  18. MECHANISMS OF NO-MEDIATED PROTEIN S-NITROSYLATION IN EAE
    OSCAR BIZZOZERO; Fiscal Year: 2007
    ..abstract_text> ..
  19. DOPAMINE TRANSPORTER: SUBSTRATE & COCAINE BINDING SITES
    JONATHAN JAVITCH; Fiscal Year: 2007
    ..4) To map the intracellular and extracellular accessibility during different functional states of substituted-cysteines through IL1 and IL3 of DAT. ..
  20. BIOSYNTHESIS OF SOME MICROBIAL METABOLITES
    Ronald Parry; Fiscal Year: 2007
    ..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
  21. HIV Protease Inhibitors & Glucose Transport
    Mike Mueckler; Fiscal Year: 2007
    ..unreadable] 2) To test the hypothesis that PI-mediated inhibition of glucose transport directly contributes to lipodystrophy by suppressing adipogenesis and/or by enhancing adipocyte apoptosis. [unreadable] [unreadable] [unreadable]..
  22. Proteomic Phosphopeptide Chip Technology for Protein Profiling
    Xiaolian Gao; Fiscal Year: 2006
    ..These capabilities of reliable measurement of proteins as a function of disease states are essential for cancer research, diagnosis and treatment. ..
  23. Parallel DNA Assembling on MicroChip
    Xiaolian Gao; Fiscal Year: 2007
    ..A reliable, miniaturized, robust process will then replace the current slow, expensive, and still largely unpredictable gene synthesis processes. [unreadable] [unreadable] [unreadable]..
  24. MOLECULAR MECHANISM OF RENAL NA+ DEPENDENT COTRANSPORT
    Ana M Pajor; Fiscal Year: 2010
    ..Understanding how NaDC1 and SdcS work will help us to develop treatments for NaDC1-mediated diseases. ..
  25. Liberation of Intracellular Zinc and Neuronal Cell Death
    Elias Aizenman; Fiscal Year: 2007
    ..With this information, we hope to uncover novel therapeutic strategies to prevent or halt the progression of these and other neurodegenerative conditions. ..
  26. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2008
    ..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. [unreadable] [unreadable] [unreadable] [unreadable]..
  27. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  28. Discovery of Novel Pharmacotherapies: Cocaine Dependence
    PETER MELTZER; Fiscal Year: 2008
    ..vi) analogues of pyrovalerone. ..
  29. Insulin Signaling in a Cell-Free System
    Mike Mueckler; Fiscal Year: 2008
    ..abstract_text> ..
  30. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  31. Leptin As A Novel Neurohormonal Anticonvulsant
    KWEE THIO; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  32. MOLECULAR RECOGNITION OF ANTIPSYCHOTIC DRUGS AND COCAINE
    JONATHAN JAVITCH; Fiscal Year: 2006
    ..abstract_text> ..
  33. Molecular determinants of ACTH receptor for ligand binding
    Yingkui Yang; Fiscal Year: 2006
    ..The information will serve to establish a foundation on which to build a comprehensive understanding of the signaling events that regulate ACTH mediated adrenal function in physiologic and diseased states. ..
  34. Involvement of Na/Dicarboxylate Cotransporter in Aging
    Ana Pajor; Fiscal Year: 2002
    ..abstract_text> ..
  35. ARCHAEAL & BACTERIAL HOMOLOGS OF DOPAMINE TRANSPORTER
    JONATHAN JAVITCH; Fiscal Year: 2002
    ....
  36. NMR AND UV STUDIES OF ANTISENSE OLIGONUCLEOTIDES
    Xiaolian Gao; Fiscal Year: 2002
    ..abstract_text> ..
  37. PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESIS
    JOHN BIELICKI; Fiscal Year: 2002
    ..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
  38. Modulation of calcium signaling by presenilin-1
    Peter Koulen; Fiscal Year: 2003
    ..Thus, potential new targets for treating those devastating conditions affecting the aging population may be identified. ..
  39. NITRIC OXIDE, PLP ACYLATION & THE PATHOPHYSIOLOGY OF MS
    OSCAR BIZZOZERO; Fiscal Year: 2003
    ..abstract_text> ..
  40. MODULATION OF NMDA RECEPTORS
    Elias Aizenman; Fiscal Year: 2003
    ..In addition, the investigations proposed here will focus on a novel form of modulation of NMDA receptor function, which may be important in retinal physiology. ..
  41. Bacterial Cell Surfaces Gordon Conference
    Barry Rosen; Fiscal Year: 2004
    ..abstract_text> ..
  42. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  43. Lacrimal gland function-regulation by calcium signaling
    Peter Koulen; Fiscal Year: 2004
    ..abstract_text> ..
  44. Development of a Peptide Microchip System
    Xiaolian Gao; Fiscal Year: 2005
    ..We will develop the chemistry to prepare these chips in Phase I and develop the system using these chips in a biologically important application in Phase II. ..
  45. Links between Mismatch Repair and Replication
    Hernan Flores Rozas; Fiscal Year: 2005
    ....
  46. ApoA-I Helical Domains and Cardiovascular Aging
    JOHN BIELICKI; Fiscal Year: 2005
    ..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
  47. 2004 & 2006 Membrane Transport Proteins Gordon Conference
    JONATHAN JAVITCH; Fiscal Year: 2005
    ..In keeping with the GRC tradition, the interactions that will result, whether from formal or informal discussions, can be expected to be extraordinarily stimulating and productive. ..
  48. Confocal Microscope for Campus Expansion in the CBH building
    Peter Koulen; Fiscal Year: 2006
    ..The current application will primarily serve eight major groups around principal investigators that are extramurally funded by peer-reviewed grants from different NIH institutes. [unreadable] [unreadable] [unreadable]..
  49. Mechanisms of Inhibitory Glycine Receptor Modulation
    KWEE THIO; Fiscal Year: 2006
    ..abstract_text> ..
  50. BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDS
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..