Genomes and Genes
Summary: Compound such as LUMINESCENT PROTEINS that cause or emit light (PHYSICAL LUMINESCENCE).
Publications222 found, 100 shown here
- Applications of luminescent inorganic and organometallic transition metal complexes as biomolecular and cellular probesKenneth Kam Wing Lo
Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, P R China
Dalton Trans 41:6021-47. 2012..Emphasis is put on the structural features, photophysical behavior, biomolecular interactions, cellular uptake, and intracellular localization properties of luminescent transition metal complexes...
- Bright monomeric red fluorescent protein with an extended fluorescence lifetimeEkaterina M Merzlyak
Evrogen JSC, Miklukho Maklaya 16 10, Moscow 117997, Russia
Nat Methods 4:555-7. 2007..These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications...
- Design and synthesis of iridium(III) azacrown complex: application as a highly sensitive metal cation phosphorescence sensorMei Lin Ho
Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan
Org Biomol Chem 4:98-103. 2006..Remarkable differentiation in spectral properties upon metal cation (e.g. Ca2+) complexation makes complex a highly sensitive phosphorescence probe...
- Phosphorescence imaging of living cells with amino acid-functionalized tris(2-phenylpyridine)iridium(III) complexesPeter Steunenberg
Supramolecular Chemistry and Technology, MESA Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE, Enschede, The Netherlands
Inorg Chem 51:2105-14. 2012....
- Label-free luminescent oligonucleotide-based probesDik Lung Ma
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Chem Soc Rev 42:3427-40. 2013..Based on the numerous examples of label-free luminescent DNA-based probes reported recently, we envisage that this field would continue to thrive and mature in the years to come...
- Ratiometric phosphorescence imaging of Hg(II) in living cells based on a neutral iridium(III) complexYongquan Wu
Department of Chemistry, Fudan University, Shanghai, 200433, PR China
Inorg Chem 50:7412-20. 2011..Importantly, cell imaging experiments have shown that [Ir(bt)(2)(acac)] is membrane permeable and can be used to monitor the changes in Hg(II) levels within cells in a ratiometric phosphorescence mode...
- Use of bioluminescent imaging to investigate the role of nuclear factor-kappaBeta in experimental non-small cell lung cancer metastasisGeorgios T Stathopoulos
Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
Clin Exp Metastasis 25:43-51. 2008..In conclusion, using bioluminescent detection of NF-kappaB activation in mouse lug adenocarcinoma cells, we found a negative impact of p65/RelA on NSCLC metastasis...
- Structure and spectroscopy of oxyluciferin, the light emitter of the firefly bioluminescencePance Naumov
Department of Material and Life Science, and Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, 2 1 Yamada oka, Suita, Osaka, Japan
J Am Chem Soc 131:11590-605. 2009..This conclusion is supported by the bioluminescence/fluorescence spectra and the NMR data, as well the crystal structures of OxyLH(2) and MOxyLH(2), where the conjugated acid (phenol) of the emitter exists as pure enol tautomer...
- Red-emitting luciferases for bioluminescence reporter and imaging applicationsBruce R Branchini
Department of Chemistry, Connecticut College, New London, CT 06320, USA
Anal Biochem 396:290-7. 2010..These results suggest that the novel enzymes should provide superior performance in dual-color reporter and in vivo imaging applications, and they illustrate the importance of codon optimization for assays in mammalian cells...
- Structure, photophysical and electrochemical properties, biomolecular interactions, and intracellular uptake of luminescent cyclometalated iridium(III) dipyridoquinoxaline complexesKenneth Yin Zhang
Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, PR China
Inorg Chem 49:2530-40. 2010..The results revealed that the complexes bound to the hydrophobic pockets of proteins, intercalated into the base-pairs of double-stranded DNA, but did not appear to interact with RNA...
- Developing red-emissive ruthenium(II) complex-based luminescent probes for cellular imagingRun Zhang
State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China
Bioconjug Chem 23:725-33. 2012..The results suggest that the new probe could be a very useful tool for luminescence imaging analysis of the toxic thiophenol in intact cells...
- Leishmania (Viannia) panamensis: an in vitro assay using the expression of GFP for screening of antileishmanial drugRubén E Varela M
Programa de Estudio y Control de Enfermedades Tropicales PECET, Universidad de Antioquia, Calle 62 No 52 59, Lab 632, Medellin, Colombia
Exp Parasitol 122:134-9. 2009..The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus...
- A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein stainingDik Lung Ma
Department of Applied Biology and Chemical Technology, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
Angew Chem Int Ed Engl 47:3735-9. 2008
- Bioluminescence monitoring of intracranial glioblastoma xenograft: response to primary and salvage temozolomide therapyEduard B Dinca
Neuroscience Graduate Program, Mayo Clinic, Rochester, Minnesota, USA
J Neurosurg 107:610-6. 2007..In the current study we have applied BLI to the analysis of clinically relevant issues involving use of the DNA methylating agent temozolomide (TMZ) in a mouse model...
- Optical imaging of in vivo gene expression: a critical assessment of the methodology and associated technologiesM Golzio
IPBS CNRS UMR 5089, Toulouse, France
Gene Ther 11:S85-91. 2004..The present paper describes the physical and technological methodologies and associated problems of in vivo optical imaging. Several examples of in vivo detection of gene delivery are described...
- Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteinsBeatrice I Lindhout
Institute of Biology Leiden, Department of Molecular and Developmental Genetics, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
Nucleic Acids Res 35:e107. 2007..Our data demonstrate that live cell imaging of specific DNA sequences can be achieved with artificial zinc finger proteins in different organisms...
- New approaches to in-cell detection of protein activity: genetically encoded chemiluminescence probes pave the way to robust HTS assaysLouis Hodgson
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris ParkAvenue, Price Center 217, Bronx, New York 10461, USA
ACS Chem Biol 3:335-7. 2008..The biosensor design strategies are highly generalizable and will be extremely valuable for expanding the repertoire of screenable targets in living cell systems...
- Systemically transferred hematopoietic stem cells home to the subretinal space and express RPE-65 in a mouse model of retinal pigment epithelium damagePelin Atmaca-Sonmez
Department of Ophthalmology and Visual Sciences, University of Louisville, 301 E Muhammad Ali Blvd, Louisville, KY 40202, USA
Exp Eye Res 83:1295-302. 2006..Furthermore, the RPE-specific marker RPE-65 was expressed on adoptively transferred HSC in the denuded areas...
- Subcellular imaging of cancer cells in live miceRobert M Hoffman
AntiCancer, Inc, San Diego, USA
Methods Mol Biol 411:121-9. 2007..Dual-color in vivo cellular imaging was also used to visualize trafficking, nuclear-cytoplasmic dynamics, and the viability of cancer cells after their injection into the portal vein of mice...
- Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinaseJeong Sun Ju
Department of Biology, Saint Louis University, 3507 Laclede Avenue, St Louis, MO 63103, USA
Am J Physiol Cell Physiol 292:C564-72. 2007....
- Effects of alginate inclusion on the vector properties of chitosan-based nanoparticlesKimberly L Douglas
Department of Biomedical Engineering, McGill University, 3775 University St, Montreal, QC, Canada H3A 2B4
J Control Release 115:354-61. 2006..Overall, alginate inclusion improved the vector properties of chitosan-based nanoparticles, demonstrating superior transfection ability while maintaining biocompatibility and low toxicity...
- Sustained transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapyKotaro Nishida
Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
Spine (Phila Pa 1976) 31:1415-9. 2006..In vivo studies using a rat model were performed to determine the feasibility of microbubble-enhanced ultrasound gene transfer technique to the intervertebral disc...
- In vivo oxygen imaging using green fluorescent proteinEiji Takahashi
Department of Physiology, Yamagata University School of Medicine, Yamagata, Japan
Am J Physiol Cell Physiol 291:C781-7. 2006..We conclude that the present technique using GFP as an oxygen indicator may allow in vivo molecular imaging of oxygen in organs...
- Aequorin luminescence-based assay for 5-hydroxytryptamine (serotonin) type 3 receptor characterizationJutta Walstab
Institute of Pharmacology and Toxicology, University of Bonn, 53113 Bonn, Germany
Anal Biochem 368:185-92. 2007..In conclusion, the aequorin assay described here provides a convenient and highly sensitive method for functional characterization of 5-HT(3) receptors that is well suited for high-throughput screening...
- Functional conservation of Pax6 regulatory elements in humans and mice demonstrated with a novel transgenic reporter mouseDavid A Tyas
Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, UK
BMC Dev Biol 6:21. 2006..The sequence of the Pax6/PAX6 protein is identical in mice and humans and previous studies have shown structural conservation of the gene's regulatory regions...
- Immunogenicity and antigenicity of allogeneic amniotic epithelial transplants grafted to the cornea, conjunctiva, and anterior chamberMingcong Wang
Department of Ophthalmology, Nippon Medical School, Sendagi, Tokyo, Japan
Invest Ophthalmol Vis Sci 47:1522-32. 2006..To determine the immunogenic characterization of amniotic epithelium (AE), by examining the fate of allogeneic AE grafts heterotopically transplanted in the eye...
- Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjectsPritha Ray
Molecular Imaging Program at Stanford, Department of Radiology, Bio X Program, Stanford University, USA
Methods Mol Biol 411:131-44. 2007..Here we describe the methods and applications of firefly and renilla luciferases in molecular imaging using small animals...
- Light threshold-controlled cone alpha-transducin translocationJunping Chen
Oklahoma Center for Neuroscience, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
Invest Ophthalmol Vis Sci 48:3350-5. 2007....
- Influence of plasmid DNA topology on the transfection properties of DOTAP/DOPE lipoplexesKatrien Remaut
Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
J Control Release 115:335-43. 2006..It seems that, compared to OC and linearized pDNA, SC pDNA is better suited to reach the perinuclear region, a prerequisite to become entrapped in the nuclei of the cells during cell division...
- Intraoperative localization of lymph node metastases with a replication-competent herpes simplex virusPrasad S Adusumilli
Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
J Thorac Cardiovasc Surg 132:1179-88. 2006..This study investigates the potential to identify lymph node metastases intraoperatively by using herpes-guided cancer cell-specific expression of green fluorescent protein...
- Second-generation triple reporter for bioluminescence, micro-positron emission tomography, and fluorescence imagingAparna H Kesarwala
Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
Mol Imaging 5:465-74. 2006..This second-generation triple reporter incorporating enhanced components enables bioluminescence, PET, and fluorescence imaging of cells and living animals...
- Enhanced transfection of tumor cells in vivo using "Smart" pH-sensitive TAT-modified pegylated liposomesAmit A Kale
Department of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA 02115, USA
J Drug Target 15:538-45. 2007..This result can be considered as an important step in the development of tumor-specific stimuli-sensitive drug and gene delivery systems...
- A double-transgenic mouse used to track migrating Schwann cells and regenerating axons following engraftment of injured nervesAyato Hayashi
Division of Plastic and Reconstructive Surgery, Washington University in St Louis School of Medicine, 660 South Euclid Ave Campus Box 8238, St Louis, MO 63110, USA
Exp Neurol 207:128-38. 2007..Live imaging of the double-transgenic thy1-CFP(23)/S100-GFP murine line enabled serial assessment of Schwann cell-axonal relationships in traumatic nerve injuries reconstructed with acellular nerve allografts...
- AAV-mediated expression targeting of rod and cone photoreceptors with a human rhodopsin kinase promoterShahrokh C Khani
Department of Ophthalmology, Ross Eye Institute, State University of New York, Buffalo, New York 14215, USA
Invest Ophthalmol Vis Sci 48:3954-61. 2007..RK, also known as G protein-coupled receptor kinase 1 (GRK1), is a component of the light adaptation pathway expressed in rods and cones...
- Visualizing the dynamics of p21(Waf1/Cip1) cyclin-dependent kinase inhibitor expression in living animalsNaoko Ohtani
Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
Proc Natl Acad Sci U S A 104:15034-9. 2007..These data illustrate the unique utility of bioluminescence imaging in advancing our understanding of the timing and, hence, likely roles of specific gene expression in higher eukaryotes...
- Quantitative imaging of gene expression in individual bacterial cells by chemiluminescenceYun Zhang
Ames Laboratory USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
Anal Chem 80:597-605. 2008..In this scheme, the overall sensitivity of the system approaches the single protein molecule level. Quantitative studies of gene expression in BL21 and XLU102 bacteria can thus be performed...
- Imaging the effects of castration on bone turnover and hormone-independent prostate cancer colonization of boneN A Cross
Biomedical Research Centre, Sheffield Hallam University, Sheffield, UK
Prostate 68:1707-14. 2008..We have tested this hypothesis using a xenograft model of early growth of prostate cancer in bone...
- Continuous delivery of D-luciferin by implanted micro-osmotic pumps enables true real-time bioluminescence imaging of luciferase activity in vivoShimon Gross
Molecular Imaging Center, Mallinckrodt Institute of Radiology, Department of Molecular Biology and Pharmacology, Washington University in St Louis, St Louis, MO 63110, USA
Mol Imaging 6:121-30. 2007..We conclude that implanted pumps provide reliable, prolonged substrate delivery for high temporal resolution BLI, traversing complications of repetitive substrate injections...
- Increased level of extracellular ATP at tumor sites: in vivo imaging with plasma membrane luciferasePatrizia Pellegatti
Department of Experimental and Diagnostic Medicine, Section of General Pathology, Interdisciplinary Center for the Study of Inflammation, University of Ferrara, Ferrara, Italy
PLoS ONE 3:e2599. 2008....
- In vivo optical bioluminescence imaging of collagen-supported cardiac cell graftsIngo Kutschka
Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California, USA
J Heart Lung Transplant 26:273-80. 2007..In this study we introduce two new experimental models for longitudinal in vivo survival studies of cardiac cell grafts using optical bioluminescence imaging...
- 5-Fluorouracil ointment for the treatment of otitis media with effusionShin Ichi Kanemaru
Department of Otolaryngology Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan
Laryngoscope 117:215-9. 2007..We estimated the depressant action of 5-FU on normal cell proliferation in vitro. In addition, clinically, we assessed whether 5-FU has the potential to prolong the effect of myringotomy...
- Degradation of pteridine reductase 1 (PTR1) enzyme during growth phase in the protozoan parasite Leishmania donovaniPranav Kumar
Drug Target Discovery and Development Division, Central Drug Research Institute, Lucknow, India
Exp Parasitol 116:182-9. 2007..Therefore, this finding has importance to identify new target molecule like the proteasome for therapeutic intervention...
- Sorbitol causes preferential selection of Muller glial precursors from late retinal progenitor cells in vitroTasneem Zahir
Schepens Eye Research Institute, Harvard Medical School, Boston, MA, USA
Mol Vis 12:1606-14. 2006..The present study was undertaken to investigate the effects of sorbitol on in vitro selection of Müller glial precursors from expanded late retinal progenitor cells (RPCs)...
- Circulating monocytes engraft in the brain, differentiate into microglia and contribute to the pathology following meningitis in miceMarija Djukic
Department of Neurology, Georg August University, Gottingen, Germany
Brain 129:2394-403. 2006..Thus, circulating cells entering the brain such as monocytes/macrophages might provide a potential cellular target for the treatment of the tissue damage following meningitis via peripheral cell therapy...
- Applications of molecular imaging in cancer gene therapyMeera Iyer
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles 90095, USA
Curr Gene Ther 5:607-18. 2005..Coupling PET to gene therapy of cancer has already been implemented in several clinical studies. This approach would help to improve the efficacy and safety of future gene therapy clinical trials...
- In vivo imaging with fluorescent proteins: the new cell biologyRobert M Hoffman
AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
Acta Histochem 106:77-87. 2004....
- Luminescence literature. GFP and imaging 2001 literatureL J Kricka
Luminescence 20:57-62. 2005
- In vivo optical imaging of human adenoid cystic carcinoma cell metastasisTao Xiong
The Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China
Oral Oncol 41:709-15. 2005..The construction of highly fluorescent and stable GFP transfectants of ACC-M has revealed the multi-organ metastatic capability of ACC-M cells through this optical imaging...
- Quantitative methods to analyze subnuclear protein organization in cell populations with varying degrees of protein expressionTy C Voss
University of Virginia Health Sciences Center, Departments of Medicine and Cell Biology, Charlottesville, Virginia 22908, USA
J Biomed Opt 10:024011. 2005..This integrated quantitative image analysis method will allow the rigorous comparison of different experimental cell populations that express variable levels of FP fusion proteins...
- Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferaseM V Zamaraeva
Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444 8585, Japan
Cell Death Differ 12:1390-7. 2005..Thus, it is suggested that the cells die with increased cytosolic ATP, and elevation of cytosolic ATP level is a requisite to the apoptotic cell death process...
- Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortexRikita Araki
Department of Developmental Biology and Neurosciences, Tohoku University Graduate School of Life Sciences, and CREST, JST, Sendai, Japan
Genesis 42:53-60. 2005..These transgenic lines are expected to facilitate morphological and physiological studies of presynaptic terminals in a variety of regions of the brain...
- Whole-body bioluminescent imaging of human uveal melanoma in a new mouse model of local tumor growth and metastasisIrene C Notting
Department of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, 2300 RC Leiden, The Netherlands
Invest Ophthalmol Vis Sci 46:1581-7. 2005..Therefore, the development of novel anticancer strategies is urgent, and more sensitive and less invasive methods of detecting and monitoring in vivo tumor growth and metastatic disease in cancer models are needed...
- Development of a fluorescent reporter to assess iron regulatory protein activity in living cellsRebecca J Henderson
Department of Pharmacology, College of Medicine, Pennsylvania State University, 500 University Drive, Hershey, Pennsylvania 17033, USA
Biochim Biophys Acta 1743:162-8. 2005....
- Time course of mycobacterial infection of dendritic cells in the lungs of intranasally infected miceR Reljic
Department for Oral Medicine and Pathology, Guy s Campus of King s College, London, UK
Tuberculosis (Edinb) 85:81-8. 2005..In this paper we investigated if their early infection in the lungs represents a plausible alternative to cross-priming with mycobacterial antigens acquired from infected macrophages...
- ABCG2/BCRP expression modulates D-Luciferin based bioluminescence imagingYimao Zhang
Russell H Morgan Department of Radiology, The Johns Hopkins Bloomberg School of Public Health, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Cancer Res 67:9389-97. 2007..These findings highlight the need to consider ABCG2/BCRP effects during d-luciferin-based BLI and suggest novel high throughput methods for identifying new ABCG2/BCRP inhibitors...
- Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic miceHanns Ulrich Zeilhofer
Department of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen Nurnberg, 91054 Erlangen, Germany
J Comp Neurol 482:123-41. 2005....
- Development of the "Cell Chip": a new in vitro alternative technique for immunotoxicity testingErik Ullerås
Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
Toxicology 206:245-56. 2005..The major advantage of this approach is the possibility to apply this test in high throughput screening of high number of compounds for their well defined biological activity...
- Botulinum neurotoxin type D enables cytosolic delivery of enzymatically active cargo proteins to neurones via unfolded translocation intermediatesSteffen Bade
Institute fur Biochemie, Medizinische Hochschule Hannover, Hannover, Germany
J Neurochem 91:1461-72. 2004..Thus, enzymatically inactive clostridial neurotoxins may serve as effective, safe carriers for delivering proteins in functionally active form to the cytosol of neurones...
- Neurally selected embryonic stem cells induce tumor formation after long-term survival following engraftment into the subretinal spaceStefan Arnhold
Department of Anatomy I, University of Cologne, Cologne, Joseph Stelzmann Strasse 9, 50931 Koln, Germany
Invest Ophthalmol Vis Sci 45:4251-5. 2004..To determine whether transplantation of embryonic stem (ES) cells into the subretinal space of rhodopsin-knockout mice has a tumorigenic effect...
- Alteration of neuronal firing properties after in vivo experience in a FosGFP transgenic mouseAlison L Barth
Department of Biological Sciences and Center for the Neural Basis of Cognition, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
J Neurosci 24:6466-75. 2004..In addition, because activated neurons can be functionally characterized, this tool may enable the development of better pharmaceuticals that directly affect the neurons involved in disease states...
- Dendritic spine abnormalities in amyloid precursor protein transgenic mice demonstrated by gene transfer and intravital multiphoton microscopyTara L Spires
Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
J Neurosci 25:7278-87. 2005..Decreased spine density will likely contribute to altered neural system function and behavioral impairments observed in Tg2576 mice...
- Visualizing levels of osteoblast differentiation by a two-color promoter-GFP strategy: Type I collagen-GFPcyan and osteocalcin-GFPtpzI Bilic-Curcic
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut
Genesis 43:87-98. 2005..It should be useful to identify and isolate populations of cells within an osteoblast lineage as they progress through stages of differentiation...
- Hepatocyte growth factor induces angiogenesis in injured lungs through mobilizing endothelial progenitor cellsKota Ishizawa
Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai, Miyagi 980 8574, Japan
Biochem Biophys Res Commun 324:276-80. 2004..These complex effects induced by HGF orchestrate pulmonary regeneration in emphysematous lung parenchyma...
- Noninvasive visualization of molecular events in the mammalian zygoteKazuo Yamagata
Graduate School of Life and Environmental Science, and Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan
Genesis 43:71-9. 2005....
- Mechanisms of neuropeptide Y, peptide YY, and pancreatic polypeptide inhibition of identified green fluorescent protein-expressing GABA neurons in the hypothalamic neuroendocrine arcuate nucleusClaudio Acuna-Goycolea
Department of Neurosurgery, Yale University, New Haven, Connecticut 06520, USA
J Neurosci 25:7406-19. 2005..Together, these results show that the family of neuropeptide Y modulators reduces the activity of inhibitory GABA neurons in the ARC by multiple presynaptic and postsynaptic mechanisms...
- Reduction of astrogliosis by early treatment of pneumococcal meningitis measured by simultaneous imaging, in vivo, of the pathogen and host responseJagath L Kadurugamuwa
Clorox Services Company, 7200 Johnson Dr, Pleasanton, CA 94588, USA
Infect Immun 73:7836-43. 2005..This model has potential for simultaneous multiparameter monitoring and testing of therapies that target the pathogen or host response to prevent neuronal injury and recovery...
- Virally-directed fluorescent imaging (VFI) can facilitate endoscopic stagingP S Adusumilli
Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Surg Endosc 20:628-35. 2006..We sought to determine the feasibility of GFP-guided imaging technology in the intraoperative detection of small tumor nodules...
- Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variantKiryl D Piatkevich
Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Bronx, New York 10461, United States
Sci Rep 3:1847. 2013..Based on the spectroscopic, biochemical, and structural analysis we suggest that the rearrangement of the hydrogen bond interactions between the chromophore and the protein matrix is responsible for the TagRFP675 spectral properties...
- An effective approach to enhanced energy-transfer efficiency from up-converting phosphors and increased assay sensitivityYunxia Yuan
Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P R China
Chem Commun (Camb) 48:7510-2. 2012..The energy transfer efficiency from up-converting donors was significantly enhanced by constructing beacon sensors, which led to increased sensitivities in DNA recognition and protein determination...
- Measurement of cell respiration and oxygenation in standard multichannel biochips using phosphorescent O2-sensitive probesAlina V Kondrashina
Biochemistry Department, University College Cork, Cork, Ireland
Analyst 138:4915-21. 2013..It also allows re-perfusion and multiple treatments of cells and multi-parametric analyses in conjunction with other probes. Optical measurements are conducted on standard fluorescence readers and microscopes. ..
- Endogenous bone-marrow-derived stem cells contribute only a small proportion of regenerated myocardium in the acute infarction modelShinya Fukuhara
Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center, Osaka, 565 8565, Japan
J Heart Lung Transplant 24:67-72. 2005..In this study, we verified the proportional contribution of cells from bone marrow (BM) and from non-bone marrow (n-BM) in regenerating neomyocardium after myocardial infarction...
- Interdigitated microelectrode array-coupled bipolar semiconductor photodiode array (IMEA-PDA) microchip for on-chip electrochemiluminescence detectionSukdeb Pal
Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151 742, South Korea
Biomed Microdevices 11:971-80. 2009..These in turn bring in speed and statistical confidence in analysis. The IMEA-PDA microchip system has the potential to be used as a versatile and highly compact chemical analysis tool for chemical sensing and metrology applications...
- Isoquinoline-based lanthanide complexes: bright NIR optical probes and efficient MRI agentsFabien Caillé
Centre de Biophysique Moleculaire, UPR 4301 CNRS, rue Charles Sadron, 45071 Orleans, France
Inorg Chem 51:2522-32. 2012....
- Clofibrate and dalargin increase luminol-dependent chemiluminescence of mouse bloodV M Mushtakova
N N Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow
Bull Exp Biol Med 144:317-8. 2007..Their combined use several-fold potentiated this effect. It is expected that combined use of hypolipidemics and polypeptides will open a new trend in the search for stimulators of oxygen-dependent nonspecific immunity...
- Novel differences in the expression of inflammation-associated genes between mid- and late-gestational dermal fibroblastsBrian C Wulff
Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA
Wound Repair Regen 21:103-12. 2013..Furthermore, baseline differences in the expression of inflammatory genes by fibroblasts in E15 and E18 skin may contribute to inflammation and scar formation late in gestation...
- Optimizing sensitization processes in dinuclear luminescent lanthanide oligomers: selection of rigid aromatic spacersJean François Lemonnier
Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, 30 quai E Ansermet, CH 1211 Geneva 4, Switzerland
J Am Chem Soc 133:16219-34. 2011..A simple interpretation of the complete set of rate constants characterizing the energy migration mechanisms provides straightforward objective criteria for the selection of [Eu2(L4)(hfac)6] as the most promising building block...
- Assessment of golgi apparatus versus plasma membrane-localized multi-drug resistance-associated protein 1Allyn M Kaufmann
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA
Mol Pharm 5:787-94. 2008..Additionally, we show that a number of human leukemic cell lines appear to have an inefficient Golgi apparatus to PM secretory pathway that could be responsible for the Golgi localization of MRP1...
- A novel luminol chemiluminescent method catalyzed by silver/gold alloy nanoparticles for determination of anticancer drug flutamideMohammad Javad Chaichi
Faculty of Chemistry, Mazandaran University, Babolsar 47416 95447, Iran Electronic address
Spectrochim Acta A Mol Biomol Spectrosc 116:594-8. 2013..This work is introduced as a new method for the determination of flutamide in commercial tablets. Box-Behnken experimental design is applied to investigate and validate the CL measurement parameters...
- Bioactive luminescent transition-metal complexes for biomedical applicationsDik Lung Ma
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Angew Chem Int Ed Engl 52:7666-82. 2013....
- IL-10 secretion increases signal persistence of HEMA-MMA-microencapsulated luciferase-modified CHO fibroblasts in miceMartin Surzyn
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Tissue Eng Part A 15:127-36. 2009..Longer duration effects require further investigation to extend this proof-of-concept study...
- Aqueous Ln(III) luminescence agents derived from a tasty precursorChristoph J Jocher
Department of Chemistry, University of California, Berkeley, California 94720, USA
Inorg Chem 47:7951-3. 2008..04%), which can be rationalized by the similar energy of the ligand triplet state and the Eu(III) (5)D0 emissive level. Instead, sensitized emission from the Yb(III) and Nd(III) cations was observed, which emit in the NIR...
- Phosphorescent imaging of living cells using a cyclometalated iridium (III) complexDik Lung Ma
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
PLoS ONE 8:e55751. 2013..The iridium(III) solvato complex [Ir(phq)2(H2O]2)] preferentially stains the cytoplasm of both live and dead cells with a bright luminescence...
- Stringent doxycycline-dependent control of gene activities using an episomal one-vector systemGeorg W Bornkamm
GSF Institut für Klinische Molekularbiologie und Tumorgenetik, Marchioninistrasse 25, D 81377 Munchen, Germany
Nucleic Acids Res 33:e137. 2005..Tight down regulation is achieved through binding of the silencer tTS(KRAB) to P(tet)bi-1 in the absence of Dox. Addition of Dox releases repression and via binding of rtTA2(S)-M2 activates P(tet)bi-1...
- Spiro-configured bipolar host materials for highly efficient electrophosphorescent devicesSung Yu Ku
Department of Chemistry, National Taiwan University, Taipei, Taiwan
Chem Asian J 7:133-42. 2012..65, 0.35) and remarkably high efficiencies of 20.3 % (21 cd A(-1)) and 13.5 Lm W(-1) at a practical brightness of 1000 cd m(-2)...
- Concentration-independent pH detection with a luminescent dimetallic Eu(III)-based probeJeremiah D Moore
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
J Am Chem Soc 134:17372-5. 2012..Modulation of the luminescence-decay rate is independent from the concentration of Eu(III), which we expect to be useful in the non-invasive imaging of in vivo pH...
- The quantum cutting of Tb(3+) in Ca(6)Ln(2)Na(2)(PO(4))(6)F(2) (Ln = Gd, La) under VUV-UV excitation: with and without Gd(3+)Mubiao Xie
MOE Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat Sen University, Guangzhou 510275, PR China
Inorg Chem 49:11317-24. 2010..In addition, the energy transfer process from Gd(3+) to Tb(3+) as well as (5)D(3)-(5)D(4) cross relaxation was investigated and discussed in terms of luminescence spectra and decay curves...
- Adsorption characteristics of spiropyran-modified cationic surfactants at the silica/aqueous solution interfaceKenichi Sakai
Department of Pure and Applied Chemistry in Faculty of Science and Technology and Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
Langmuir 26:9283-8. 2010..By taking the in situ and ex situ color changes into consideration, it seems likely that the photoisomerization occurs reversibly even after surfactant adsorption on silica...
- Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigeninT V Zhidkova
Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia
Anal Bioanal Chem 401:381-6. 2011..A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics...
- Photoluminescent biocompatible silicon nanoparticles for cancer theranostic applicationsLiubov A Osminkina
Department of Physics, Lomonosov Moscow State University, 119992 Moscow, Russia
J Biophotonics 5:529-35. 2012..These properties of SiNPs are discussed in view of possible applications in theranostics (both in therapy and in diagnostics)...
- Microflow-injection chemiluminescence of luminol and hypochlorite enhanced by phloxine BXiaoli Song
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, People s Republic of China
Luminescence 28:16-22. 2013..2% (n = 10) for 2.5 µmol/L hypochlorite. The sample consumption was only 2 μL, with a sample throughput of 90/h. The method has been used for determining trace amounts of hypochlorite in water samples with satisfactory results...
- A lanthanide-complex-based ratiometric luminescent probe specific for peroxynitriteCuihong Song
Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Chemistry 16:6464-72. 2010..The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application...
- Influence of three scan spray systems on human gingival fibroblastsInes Willershausen
Department of Operative Dentistry, University Medical Center of Johannes Gutenberg University, Mainz, Germany
Quintessence Int 43:e67-72. 2012..The aim of this in vitro study was to investigate the influence of three different scan sprays on the proliferation, viability, and adenylate kinase (ADK) release of human fibroblasts...
- Synthesis, radii dependent self-assembly crystal structures and luminescent properties of rare earth (III) complexes with a tripodal salicylic derivativeYa wen Wang
State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China
Dalton Trans 39:9013-21. 2010..The photophysical properties of the Eu and Tb complexes are investigated in the solid state at room temperature...
- A set of GFP organelle marker lines for intracellular localization studies in Medicago truncatulaBin Luo
USDA ARS Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates St, Houston, TX 77030 2600, USA
Plant Sci 188:19-24. 2012..Moreover, this set of marker lines is compatible with both transient and stable expression systems. Thus, this marker set should prove to be a useful resource for the M. truncatula research community...
- Effect of surface modification on photoluminescence properties of Y3Al5O12:Ce3+, Gd3+ nano-phosphorsJie Li
Key Laboratory of Interface Science and Engineering in Advanced Materials Taiyuan University of Technology, Ministry of Education, Taiyuan, PR China
Spectrochim Acta A Mol Biomol Spectrosc 78:1310-4. 2011....
- Functionalized 1,2,3-triazoles as building blocks for photoluminescent POLOs (polymers of oligomers) of copper(I)Shi Qiang Bai
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543
Dalton Trans 39:2631-6. 2010..Complex 3 shows a 2-D (4, 4) net with the stair-step Cu(4)I(4) as node and L3 as a building block. All three complexes exhibit photoluminescence...
- TMEM106B, the risk gene for frontotemporal dementia, is regulated by the microRNA-132/212 cluster and affects progranulin pathwaysAlice S Chen-Plotkin
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Neurosci 32:11213-27. 2012..Evidence for this pathogenic cascade includes the striking convergence of two independent, genomic-scale screens on a microRNA:mRNA regulatory pair. Our findings open novel directions for elucidating miR-based therapies in FTLD-TDP...
- Synthesis of chlorin-sensitized near infrared-emitting lanthanide complexesJohanna Laakso
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE 10691, Stockholm, Sweden
Inorg Chem 51:10366-74. 2012..The complexes benefit from dual chlorin (610-639 nm) and lanthanide (980 or 1065 nm for Yb- or Nd-complexes, respectively) emission in the biologically relevant red and near IR region of the spectrum...
- Aggregation-induced emissionYuning Hong
Department of Chemistry, Nano Science and Technology Program, Bioengineering Graduate Program, Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology HKUST, Clear Water Bay, Kowloon, Hong Kong, China
Chem Soc Rev 40:5361-88. 2011..Technological, especially optoelectronic and biological, applications of the AIE systems are exemplified to illustrate how the novel AIE effect can be utilized for high-tech innovations (183 references)...
- Maxillary sinus floor elevation using a tissue engineered bone complex with BMP-2 gene modified bMSCs and a novel porous ceramic scaffold in rabbitsX Juan Sun
Ninth People s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
Arch Oral Biol 55:195-202. 2010....
- Lanthanide directed self-assembly formations of Tb(III) and Eu(III) luminescent complexes from tryptophan based pyridyl amide ligandsChristophe Lincheneau
School of Chemistry, Centre for Synthesis and Chemical Biology, Trinity College Dublin, Dublin 2, Ireland
Chem Commun (Camb) 47:7119-21. 2011....
- Improved Safe Alternative to Semiconductor Quantum DotsROBERT BOLSKAR; Fiscal Year: 2005Quantum dots are recognized as the core materials of a new generation luminescent agents for biolabelling applications. This project will establish proof-of-concept for a new class of non-toxic, metal free quantum dot-like materials...
- Inhibitors of 5alpha-reductase for acne therapyLingna Li; Fiscal Year: 2002..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
- Adenoviral GFP targeting of metastatic human tumors using multiple delivery routeHiroyuki Kishimoto; Fiscal Year: 2008..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
- Orthotopic models of tumor angiogenesis and blood flowMeng Yang; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Therapeutic hair follicle-derived neurospheresMeng Yang; Fiscal Year: 2007..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
- Structure-based Directed Evolution of Fast-Maturing GFPsRebekka Wachter; Fiscal Year: 2007..In more general terms, a fast-maturing GFP would aid in the development of therapies for a very large number of disease states including neurodegenerative and cancerous disorders. [unreadable] [unreadable] [unreadable]..
- Dual-color tumor-host imaging modelsMeng Yang; Fiscal Year: 2007..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
- Targeted tumoricidal bacteriaMing Zhao; Fiscal Year: 2007..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
- Imageable tumor-targeting bacteriaMing Zhao; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Enzyme therapy for hyperhomocysteinemiaYuying Tan; Fiscal Year: 2005..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
- Identifying targets for diagnosing oral candidiasisDavid Schofield; Fiscal Year: 2005..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
- RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAYYuying Tan; Fiscal Year: 2002..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
- Discovery of novel fluorescent reporter genesMing Zhao; Fiscal Year: 2004..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
- Design of Gene Delivery System to Target HepatocytesMark Davis; Fiscal Year: 2008..The long-term objective of our proposal is to design and engineer a generalized, synthetic system for ultimately delivering nucleic acid-based therapeutics to hepatocytes in humans. ..
- Transcriptional Regulation of Rhodopsin Kinase GeneSHAHROKH KHANI; Fiscal Year: 2006..These novel candidate genes may also participate in the pathogenesis of human retinal degenerations. ..
- Differentiation Potential of Adult Rat Stem CellsJEFFERY SPEES; Fiscal Year: 2005..3. To determine the engraftment and differentiation potential of the stem cell sub-populations in a rat model of monocrotaline-induced pulmonary hypertension. ..
- Imaging Vaccina Viral-Host PathogenesisGary Luker; Fiscal Year: 2007..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. [unreadable] [unreadable] [unreadable]..
- Glycophorin A Dimers to Probe Membranes: A 2D-IR StudyLOUISE SINKS; Fiscal Year: 2008..Since many transmembrane proteins critical to cell function, understanding the interplay between membrane environment and protein structure is vitallv important. ..
- A Unified Reporter Gene for Multi-Modality ImagingJianghong Rao; Fiscal Year: 2005..abstract_text> ..