cross linking reagents

Summary

Summary: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.

Top Publications

  1. ncbi CLIP: a method for identifying protein-RNA interaction sites in living cells
    Jernej Ule
    Howard Hughes Medical Institute and the Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, USA
    Methods 37:376-86. 2005
  2. ncbi Chemical cross-linking and mass spectrometry to map three-dimensional protein structures and protein-protein interactions
    Andrea Sinz
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, D 04103 Leipzig, Germany
    Mass Spectrom Rev 25:663-82. 2006
  3. ncbi The Fanconi anaemia gene FANCC promotes homologous recombination and error-prone DNA repair
    Wojciech Niedzwiedz
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 15:607-20. 2004
  4. pmc DNA replication is required To elicit cellular responses to psoralen-induced DNA interstrand cross-links
    Y M Akkari
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, Oregon 97201, USA
    Mol Cell Biol 20:8283-9. 2000
  5. ncbi Modification of Cytochrome c by 4-hydroxy- 2-nonenal: evidence for histidine, lysine, and arginine-aldehyde adducts
    Amanda L Isom
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Am Soc Mass Spectrom 15:1136-47. 2004
  6. pmc BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks
    Pietro Pichierri
    UPR2169 CNRS, Genetic Instability and Cancer, Institut Gustave Roussy, Pavillon de Recherche, rue Camille Desmoulins, Villejuif, France
    EMBO J 23:3154-63. 2004
  7. ncbi Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation
    Ilja Demuth
    Molecular Genetics Program, Benaroya Research Institute, Seattle, WA 98101 2795, USA
    Oncogene 23:8611-8. 2004
  8. pmc DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zeta
    Sovan Sarkar
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 25:1285-94. 2006
  9. ncbi DNA interstrand cross-link repair in Saccharomyces cerevisiae
    Peter Lehoczký
    Department of Molecular Genetics, Cancer Research Institute, Bratislava, Slovak Republic
    FEMS Microbiol Rev 31:109-33. 2007
  10. pmc Snm1B/Apollo mediates replication fork collapse and S Phase checkpoint activation in response to DNA interstrand cross-links
    J B Bae
    Department of Cancer Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 27:5045-56. 2008

Detail Information

Publications313 found, 100 shown here

  1. ncbi CLIP: a method for identifying protein-RNA interaction sites in living cells
    Jernej Ule
    Howard Hughes Medical Institute and the Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, USA
    Methods 37:376-86. 2005
    ..Here, we present an improved protocol that performs RNA linker ligation before the SDS-PAGE step, and describe its application to the specific purification and amplification of RNA ligands of Nova in neurons...
  2. ncbi Chemical cross-linking and mass spectrometry to map three-dimensional protein structures and protein-protein interactions
    Andrea Sinz
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, D 04103 Leipzig, Germany
    Mass Spectrom Rev 25:663-82. 2006
    ..The various approaches described in the literature to facilitate the mass spectrometric detection of cross-linked products as well as computer software for data analyses are reviewed...
  3. ncbi The Fanconi anaemia gene FANCC promotes homologous recombination and error-prone DNA repair
    Wojciech Niedzwiedz
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 15:607-20. 2004
    ..These studies reveal that the FA proteins cooperate with key mutagenesis and repair processes that enable replication of damaged DNA...
  4. pmc DNA replication is required To elicit cellular responses to psoralen-induced DNA interstrand cross-links
    Y M Akkari
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, Oregon 97201, USA
    Mol Cell Biol 20:8283-9. 2000
    ..Kinetic analysis suggested that these cells could remove up to approximately 2,500 ICLs/genome at an average rate of 11 ICLs/genome/h...
  5. ncbi Modification of Cytochrome c by 4-hydroxy- 2-nonenal: evidence for histidine, lysine, and arginine-aldehyde adducts
    Amanda L Isom
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Am Soc Mass Spectrom 15:1136-47. 2004
    ....
  6. pmc BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks
    Pietro Pichierri
    UPR2169 CNRS, Genetic Instability and Cancer, Institut Gustave Roussy, Pavillon de Recherche, rue Camille Desmoulins, Villejuif, France
    EMBO J 23:3154-63. 2004
    ....
  7. ncbi Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation
    Ilja Demuth
    Molecular Genetics Program, Benaroya Research Institute, Seattle, WA 98101 2795, USA
    Oncogene 23:8611-8. 2004
    ....
  8. pmc DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zeta
    Sovan Sarkar
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 25:1285-94. 2006
    ..We show that this combination of NER and TLS is the only pathway of ICL repair available to the cell in G1 phase and is essential for viability in the presence of DNA crosslinks...
  9. ncbi DNA interstrand cross-link repair in Saccharomyces cerevisiae
    Peter Lehoczký
    Department of Molecular Genetics, Cancer Research Institute, Bratislava, Slovak Republic
    FEMS Microbiol Rev 31:109-33. 2007
    ..Here we review, with reference to similarities and differences in higher eukaryotes, what has been discovered to date concerning ICL repair in this simple eukaryote...
  10. pmc Snm1B/Apollo mediates replication fork collapse and S Phase checkpoint activation in response to DNA interstrand cross-links
    J B Bae
    Department of Cancer Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 27:5045-56. 2008
    ..In addition, we also show that Snm1B interacts with the Mre11-Rad50-Nbs1 (MRN) complex and with FancD2 further substantiating its role as a checkpoint/DNA repair protein...
  11. ncbi Identification of novel interactions in HIV-1 capsid protein assembly by high-resolution mass spectrometry
    Jason Lanman
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    J Mol Biol 325:759-72. 2003
    ..Most significantly, we have identified a previously unrecognized intersubunit N domain-C domain interaction. The detection of this interaction reconciles previously discrepant biophysical and genetic data...
  12. ncbi Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Annu Rev Biochem 73:39-85. 2004
    ..In this review the molecular mechanisms of DNA repair and the DNA damage checkpoints in mammalian cells are analyzed...
  13. ncbi Analyzing the handoff of DNA from UvrA to UvrB utilizing DNA-protein photoaffinity labeling
    Matthew J DellaVecchia
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:45245-56. 2004
    ....
  14. pmc Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking
    Oleg V Kovalenko
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Biochem J 377:407-17. 2004
    ..In conclusion, our results strongly suggest that tetraspanin homodimers, formed in the Golgi and present at the cell surface, serve as building blocks for the assembly of larger, multicomponent tetraspanin protein complexes...
  15. ncbi Mammalian telomeres end in a large duplex loop
    J D Griffith
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill and Curriculum in Genetics and Molecular Biology, 27599 7295, USA
    Cell 97:503-14. 1999
    ..T loops may provide a general mechanism for the protection and replication of telomeres...
  16. pmc Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer
    A K Kenworthy
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Cell Biol 142:69-84. 1998
    ..These findings constrain current models for lipid rafts and the membrane organization of GPI-anchored proteins...
  17. ncbi Analysis of the native quaternary structure of vanilloid receptor 1
    N Kedei
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28613-9. 2001
    ..Finally, differences in glycosylation between the systems were observed, indicating the need for caution in the use of the heterologous expression systems for analysis of VR1 properties...
  18. ncbi Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5
    C Sirrenberg
    Institut für Physiologische Chemie der Universität München, Germany
    Nature 391:912-5. 1998
    ..This interaction of Tim10 and Tim12 with the precursors depends on the presence of divalent metal ions. Both proteins contain a zinc-finger-like motif with four cysteines and bind equimolar amounts of zinc ions...
  19. pmc Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly
    L Hinck
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305 5426
    J Cell Biol 125:1327-40. 1994
    ..Subsequently, > 90% of labeled cadherin, but no additional labeled catenin complexes, entered the TX-100-insoluble fraction.(ABSTRACT TRUNCATED AT 400 WORDS)..
  20. ncbi Behavioral sensitization to cocaine is associated with increased AMPA receptor surface expression in the nucleus accumbens
    Amy C Boudreau
    Department of Neuroscience, The Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064 3095, USA
    J Neurosci 25:9144-51. 2005
    ..We propose that drug-seeking responses are more effectively triggered in cocaine-sensitized rats because of increased cell surface expression of AMPA receptors...
  21. pmc High-level expression, functional reconstitution, and quaternary structure of a prokaryotic ClC-type chloride channel
    M Maduke
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    J Gen Physiol 114:713-22. 1999
    ..Cross-linking studies argue that EriC is a homodimer in both detergent micelles and reconstituted liposomes, a conclusion corroborated by gel filtration and analytical sedimentation experiments...
  22. pmc Repair of intermediate structures produced at DNA interstrand cross-links in Saccharomyces cerevisiae
    P J McHugh
    CRC Drug DNA Interactions Research Group, Department of Oncology, Royal Free and University College Medical School, University College London, London W1P 8BT, United Kingdom
    Mol Cell Biol 20:3425-33. 2000
    ....
  23. ncbi Myosin-IXb is a single-headed and processive motor
    Penny L Post
    Department of Molecular, Yale University, New Haven, Connecticut 06520 8103, USA
    J Biol Chem 277:11679-83. 2002
    ....
  24. ncbi Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices
    Peter Angele
    Department of Trauma Surgery, University Hospital Regensburg, Franz Josef Strauss Allee 11, Regensburg 93051, Germany
    Biomaterials 25:2831-41. 2004
    ..This study also indicates that results obtained for scaffolds based on a certain collagen species may not be transferable to scaffolds based on another, because of the differing physico-chemical properties...
  25. pmc 4-thio-U cross-linking identifies the active site of the VS ribozyme
    Shawna L Hiley
    Department of Molecular and Medical Genetics, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada M5S 1A8
    EMBO J 21:4691-8. 2002
    ..This localization of the active site is consistent with previous mutational, biochemical and biophysical data, and provides direct evidence that the cleavage site in helix I interacts with the 730 loop in helix VI...
  26. ncbi Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts
    R A Sheldon
    Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands
    Biochem Soc Trans 35:1583-7. 2007
    ..g. an oxynitrilase/nitrilase combi-CLEA for the one-pot conversion of benzaldehyde into (S)-mandelic acid, in high yield and enantiomeric purity...
  27. ncbi New insights into the Fanconi anemia pathway from an isogenic FancG hamster CHO mutant
    Robert S Tebbs
    Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, P O Box 808, Livermore, CA 94551 0808, USA
    DNA Repair (Amst) 4:11-22. 2005
    ..We propose that the primary function of FA proteins is to maintain chromosomal continuity by stabilizing replication forks that encounter nicks, gaps, or replication-blocking lesions...
  28. pmc Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles
    Jedidiah Gaetz
    Laboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Ave, Box 202, New York, NY 10021, USA
    J Cell Biol 166:465-71. 2004
    ....
  29. pmc Observing FcepsilonRI signaling from the inside of the mast cell membrane
    B S Wilson
    Department of Pathology and Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, 87131, USA
    J Cell Biol 149:1131-42. 2000
    ..The sequential association of FcstraightepsilonRI with Lyn, Syk, and coated pits in topographically distinct membrane domains implicates membrane segregation in the regulation of FcstraightepsilonRI signaling...
  30. ncbi Mitochondrial Hsp70/MIM44 complex facilitates protein import
    H C Schneider
    Institut für Physiologische Chemie der Universität München, Germany
    Nature 371:768-74. 1994
    ..The complex appears to act as a molecular ratchet which is energetically driven by the hydrolysis of ATP...
  31. ncbi Crosslinked iron oxides (CLIO): a new platform for the development of targeted MR contrast agents
    Patrick Wunderbaldinger
    Center of Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Acad Radiol 9:S304-6. 2002
  32. pmc Localized torsional tension in the DNA of human cells
    M Ljungman
    Department of Biological Sciences, Stanford University, CA 94305 5020
    Proc Natl Acad Sci U S A 89:6055-9. 1992
    ..These results document an intragenomic heterogeneity of DNA torsional tension and lend support to the twin supercoiled domain model for transcription in the genome of intact human cells...
  33. pmc Growth retardation, early death, and DNA repair defects in mice deficient for the nucleotide excision repair enzyme XPF
    Ming Tian
    Howard Hughes Medical Institute, and Department of Genetics, Harvard University Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:1200-5. 2004
    ..These phenotypes are identical to those exhibited by the ERCC1-deficient mice, consistent with the functional association of the two proteins. The complex phenotype suggests that XPF-ERCC1 is involved in multiple DNA repair processes...
  34. pmc Nucleotide excision repair- and polymerase eta-mediated error-prone removal of mitomycin C interstrand cross-links
    Huyong Zheng
    Departments of Experimental Radiation Oncology Molecular Genetics Carcinogenesis, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Cell Biol 23:754-61. 2003
    ..From these observations, we propose the existence of a recombination-independent and mutagenic repair pathway for the removal of ICLs in mammalian cells...
  35. ncbi Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle
    A Conconi
    Institute for Cell Biology, Swiss Federal Institute of Technology, Zurich
    Cell 57:753-61. 1989
    ..This suggests that the two states of chromatin are maintained independently of the transcriptional process and that they are stably propagated through the cell cycle...
  36. ncbi Chemical cross-linking and mass spectrometry for mapping three-dimensional structures of proteins and protein complexes
    Andrea Sinz
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, D 04103 Leipzig, Germany
    J Mass Spectrom 38:1225-37. 2003
    ..Cross-linking techniques combined with mass spectrometry and bioinformatic methods have the potential to provide the basis for an efficient structural characterization of proteins and protein complexes...
  37. pmc Stereospecific formation of the (R)-gamma-hydroxytrimethylene interstrand N2-dG:N2-dG cross-link arising from the gamma-OH-1,N2-propano-2'-deoxyguanosine adduct in the 5'-CpG-3' DNA sequence
    Hai Huang
    Department of Chemistry and Center in Molecular Toxicology, Vanderbilt University, Nashville, Tennessee 37235, USA
    J Am Chem Soc 131:8416-24. 2009
    ..The formation of this hydrogen bond may, in part, explain the thermal stability of this carbinolamine interstrand cross-link and the stereochemical preference for the (R) configuration of the cross-link...
  38. ncbi The cellular response to transglutaminase-cross-linked collagen
    David Y S Chau
    School of Biomedical and Natural Sciences, The Nottingham Trent University, Clifton, Nottingham, NG11 8NS, UK
    Biomaterials 26:6518-29. 2005
    ..In addition, the HOB cells were shown to differentiate at a faster rate than on native collagen when assessed by measurement of alkaline phosphatase activity and osteopontin expression...
  39. ncbi The actin cytoskeleton is required for the trafficking of the B cell antigen receptor to the late endosomes
    B K Brown
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Traffic 2:414-27. 2001
    ..Thus, BCR-trafficking requires functional actin filaments for both internalization and movement to late endosomes/lysosomes, defining critical control points in BCR-antigen targeting...
  40. ncbi The WD40 propeller domain of Cdh1 functions as a destruction box receptor for APC/C substrates
    Claudine Kraft
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 18:543-53. 2005
    ..Our data imply that APC/C is activated by the association of Cdh1 with Cdc27, which enables APC/C to recognize the D box of substrates via Cdh1's propeller domain...
  41. pmc Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
    Gurman Singh Pall
    Division of Cancer Sciences and Molecular Pathology, Glasgow University, Western Infirmary, Dumbarton Road, Glasgow, G11 6NT, UK
    Nucleic Acids Res 35:e60. 2007
    ..All types of hybridization probes tested benefited from the new cross-linking procedure. Cross-linking was dependent on a terminal phosphate and so, should be applicable to other related categories of small RNA...
  42. ncbi Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
    Arun Kalliat Thazhathveetil
    Laboratory of Molecular Gerontology, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    Bioconjug Chem 18:431-7. 2007
    ..The results indicated that the digoxigenin-TMP conjugate 7a and digoxigenin-DMA conjugate 7b can be used for studying the repair of laser localized DNA monoadducts and cross-links...
  43. ncbi Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking
    Ludovic Richert
    Institut National de la Sante et de la Recherche Medicale, Unite 595, Faculte de Chirurgie Dentaire, Universite Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France
    Biomacromolecules 5:284-94. 2004
    ..Whereas the non cross-linked films are highly resistant to cell adhesion, the cells adhere and spread well on the cross-linked films...
  44. ncbi S100A11, S100A10, annexin I, desmosomal proteins, small proline-rich proteins, plasminogen activator inhibitor-2, and involucrin are components of the cornified envelope of cultured human epidermal keratinocytes
    N A Robinson
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106 4970, USA
    J Biol Chem 272:12035-46. 1997
    ..No loricrin, cystatin A, or elafin peptides were detected...
  45. ncbi Mapping the topology and determination of a low-resolution three-dimensional structure of the calmodulin-melittin complex by chemical cross-linking and high-resolution FTICRMS: direct demonstration of multiple binding modes
    Daniela M Schulz
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, D 04103 Leipzig, Germany
    Biochemistry 43:4703-15. 2004
    ..This integrated approach, exemplified for mapping the topology of the calmodulin-melittin complex, is likely to have wide-ranging implications for structural studies on protein-protein interactions...
  46. ncbi PvuII endonuclease contains two calcium ions in active sites
    J R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Mol Biol 300:1049-56. 2000
    ....
  47. pmc Interface analysis of the complex between ERK2 and PTP-SL
    Mihaela C Balasu
    Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
    PLoS ONE 4:e5432. 2009
    ..A general observation emerging from this model is that regions involved in substrate binding in PTP-SL and ERK2, respectively are interacting within the interface of the complex...
  48. pmc Identification of cross-linked peptides from large sequence databases
    Oliver Rinner
    Institute of Molecular Systems Biology, Eidgenossische Technische Hochschule ETH Zurich, Wolfgang Pauli Strasse 16, 8093 Zurich, Switzerland
    Nat Methods 5:315-8. 2008
    ..We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search...
  49. ncbi A modular cross-linking approach for exploring protein interactions
    Michelle Trester-Zedlitz
    Laboratories of Synthetic Protein Chemistry, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    J Am Chem Soc 125:2416-25. 2003
    ..This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2...
  50. pmc Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles
    Eva Kühn-Hölsken
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    RNA 11:1915-30. 2005
    ..This type of analysis is applicable to any type of RNP complex and may be expected to pave the way for the further analysis of protein-RNA complexes in much lower abundance and/or of cross-links that are obtained in low yield...
  51. ncbi Mapping protein interfaces by a trifunctional cross-linker combined with MALDI-TOF and ESI-FTICR mass spectrometry
    Andrea Sinz
    Biotechnological Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, Germany
    J Am Soc Mass Spectrom 16:1921-31. 2005
    ..This strategy is expected to be especially beneficial for investigating large protein assemblies...
  52. ncbi Kinetics of collagen crosslinking in adult bovine articular cartilage
    T Ahsan
    Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Osteoarthritis Cartilage 13:709-15. 2005
    ..Determine the kinetics of collagen crosslinking in adult bovine articular cartilage explants using radiolabel pulse-chase studies...
  53. ncbi Chemical cross-linking with NHS esters: a systematic study on amino acid reactivities
    Stefanie Mädler
    Department of Chemistry and Applied Biosciences, ETH Zurich, CH 8093 Zurich, Switzerland
    J Mass Spectrom 44:694-706. 2009
    ..The reactivity is highly dependent on the pH and on adjacent amino acids...
  54. pmc Fludarabine-mediated suppression of the excision repair enzyme ERCC1 contributes to the cytotoxic synergy with the DNA minor groove crosslinking agent SJG-136 (NSC 694501) in chronic lymphocytic leukaemia cells
    C Pepper
    Department of Haematology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    Br J Cancer 97:253-9. 2007
    ..28; P=0.017) offering a mechanistic rationale for the synergistic interaction. The data presented here provides a clear indication that this combination of drugs may have clinical utility as salvage therapy in drug-resistant CLL...
  55. pmc Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres
    Miriam Grosse Hovest
    Department of Dermatology, University of Cologne, 50924 Cologne, Germany
    Mol Biol Cell 17:1758-67. 2006
    ..We thus identify ATR as a novel mediator of telomere-dependent senescence in response to ICL induced by photoactivated psoralens...
  56. ncbi Adenine-containing DNA-DNA cross-links of antitumor nitrogen mustards
    Shawn Balcome
    Department of Medicinal Chemistry, University of Minnesota Cancer Center, Room 760E, Box 806 Mayo, 420 Delaware Street Southeast, Minneapolis, Minnesota 55455, USA
    Chem Res Toxicol 17:950-62. 2004
    ..The presence of cross-linked adenine-adenine lesions may explain the enhanced cytotoxicity and mutagenicity of NMs in cells deficient in N3-alkyladenine glycosylase...
  57. pmc NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center
    S Wang
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    J Cell Biol 130:519-28. 1995
    ..Since NAC is not a core ribosomal protein, the emergence of nascent chains from the ribosome may be more dynamic than previously thought...
  58. ncbi Long range 1,4 and 1,6-interstrand cross-links formed by a trinuclear platinum complex. Minor groove preassociation affects kinetics and mechanism of cross-link formation as well as adduct structure
    Alexander Hegmans
    Department of Chemistry, Virginia Commonwealth University, Richmond, Virginia 23284 2006, USA
    J Am Chem Soc 126:2166-80. 2004
    ..The conformational flexibility of the cross-links is discussed in relation to their biological processing, especially protein recognition and repair, which are critical determinants of the cytotoxicity of these unique DNA-binding agents...
  59. ncbi Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)
    R Schoevaart
    Biocatalysis and Organic Chemistry, Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL, The Netherlands
    Biotechnol Bioeng 87:754-62. 2004
    ..The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated...
  60. ncbi Binding discrimination of MutS to a set of lesions and compound lesions (base damage and mismatch) reveals its potential role as a cisplatin-damaged DNA sensing protein
    Laurence Fourrier
    Centre de Biophysique Moleculaire, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Biol Chem 278:21267-75. 2003
    ....
  61. ncbi Induction of a caffeine-sensitive S-phase cell cycle checkpoint by psoralen plus ultraviolet A radiation
    Christoph Joerges
    Klinik und Poliklinik fur Dermatologie und Allergologie, Ludwig Maximilians University, Frauenlobstr 9 11, D 80337 Munich, Germany
    Oncogene 22:6119-28. 2003
    ..Overexpression of Cdc25A only partially overrode the S-phase arrest, suggesting that additional signaling events implement PUVA-induced S-phase arrest...
  62. ncbi CLIP: crosslinking and immunoprecipitation of in vivo RNA targets of RNA-binding proteins
    Kirk B Jensen
    Centre for the Molecular Genetics of Development, University of Adelaide, Adelaide, Australia
    Methods Mol Biol 488:85-98. 2008
    ....
  63. pmc Stably maintained microdomain of localized unrestrained supercoiling at a Drosophila heat shock gene locus
    E R Jupe
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267 0524
    EMBO J 12:1067-75. 1993
    ....
  64. ncbi Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells
    Laura J Niedernhofer
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 278:31426-33. 2003
    ..These experiments provide biological and biochemical evidence for the existence of MDA-induced DNA interstrand cross-links that could result from endogenous oxidative stress and likely have potent biological effects...
  65. ncbi Specialized chromatin structure domain boundary elements flanking a Drosophila heat shock gene locus are under torsional strain in vivo
    E R Jupe
    Department of Molecular Genetics, University of Cincinnati College of Medicine, Ohio 45067
    Biochemistry 34:2628-33. 1995
    ..The existence of a highly localized region of supercoiling within the scs and scs' sequences might be related to their activity in vivo as insulators of chromosomal position effects in Drosophila...
  66. ncbi Chemical cross-linking and mass spectrometry for protein structural modeling
    Jaap Willem Back
    Swammerdam Institute for Life Sciences SILS, Mass Spectrometry Group, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands
    J Mol Biol 331:303-13. 2003
    ..Based on chemical cross-linking and offering the analytical speed and sensitivity of mass spectrometry these methodologies are thought to contribute valuable tools towards future high throughput protein structure elucidation...
  67. ncbi Cytotoxicity of glutaraldehyde crosslinked collagen/poly(vinyl alcohol) films is by the mechanism of apoptosis
    Julie E Gough
    School of Biomedical Sciences, E Floor, University of Nottingham Medical School, Queen s Medical Centre, Nottingham, NG7 2UH, United Kingdom
    J Biomed Mater Res 61:121-30. 2002
    ....
  68. ncbi Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)
    H W Sung
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013
    J Biomed Mater Res 55:538-46. 2001
    ..This suggested that the toxic residues remaining in the glutaraldehyde-fixed tissue leached out slowly into its corresponding storage solution during the course of storage...
  69. ncbi Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid
    Nobumasa Hino
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Methods 2:201-6. 2005
    ..Cross-linked complexes with endogenous proteins were also detected. In vivo photo-cross-linking with pBpa incorporated in proteins will be useful for studying protein-protein interactions in mammalian cells...
  70. ncbi Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest
    Marit Otterlei
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
    J Cell Sci 119:5137-46. 2006
    ..We verify the WRN-RAD51 and WRN-RAD54B direct interaction in vitro. Our data support a role for WRN also in the recombination step of ICL repair...
  71. pmc Why are two different cross-linkers necessary for actin bundle formation in vivo and what does each cross-link contribute?
    L G Tilney
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Cell Biol 143:121-33. 1998
    ..Thus, forked aligns the filaments and then directs fascin binding so that inappropriate cross-linking does not occur...
  72. ncbi Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking
    Isabelle Migneault
    Universite de Montreal, Montreal, Canada
    Biotechniques 37:790-6, 798-802. 2004
    ....
  73. pmc NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link
    F Paquet
    Centre de Biophysique Moleculaire, CNRS UPR 4301, rue Charles Sadron, F 45071 Orleans cedex 2, France
    Nucleic Acids Res 27:4261-8. 1999
    ..These results are discussed in relation to the slow rate of closure of the monofunctional adducts into interstrand cross-links...
  74. ncbi Homodimerization of amyloid precursor protein and its implication in the amyloidogenic pathway of Alzheimer's disease
    S Scheuermann
    ZMBH, Center for Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    J Biol Chem 276:33923-9. 2001
    ..We suggest that dimerization could be a physiologically important mechanism for regulating the proposed signal activity of APP...
  75. pmc Identification and quantification of a guanine-thymine intrastrand cross-link lesion induced by Cu(II)/H2O2/ascorbate
    Haizheng Hong
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    Chem Res Toxicol 19:614-21. 2006
    ..The induction of intrastrand cross-link lesion in calf thymus DNA by Fenton reagents in vitro suggests that this type of lesion might be formed endogenously in mammalian cells...
  76. pmc T-cell activation by soluble MHC oligomers can be described by a two-parameter binding model
    J D Stone
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biophys J 81:2547-57. 2001
    ..The model can be used to predict activation and binding behavior for T cells and MHC oligomers with different properties...
  77. pmc Chromosome instability and defective recombinational repair in knockout mutants of the five Rad51 paralogs
    M Takata
    CREST Research Project, Radiation Genetics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
    Mol Cell Biol 21:2858-66. 2001
    ..Moreover, all mutants show partial correction of resistance to DNA damage by overexpression of human Rad51. We conclude that the Rad51 paralogs participate in repair as a functional unit that facilitates the action of Rad51 in HR...
  78. ncbi Recombination repair pathway in the maintenance of chromosomal integrity against DNA interstrand crosslinks
    M S Sasaki
    Radiation Biology Center, Kyoto University, Kyoto, Japan
    Cytogenet Genome Res 104:28-34. 2004
    ..The impairment of the HR pathway, which otherwise leads to cell death, may be somewhat substituted by an alternative mechanism such as the Mre11/Rad50/Nbs1 pathway, resulting in reduced frequencies of SCEs and CAs...
  79. pmc DNA-protein cross-linking by trans-[PtCl(2)(E-iminoether)(2)]. A concept for activation of the trans geometry in platinum antitumor complexes
    Olga Novakova
    Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ 61265 Brno
    Nucleic Acids Res 31:6450-60. 2003
    ....
  80. ncbi DNA repair defects channel interstrand DNA cross-links into alternate recombinational and error-prone repair pathways
    Wilma A Saffran
    Department of Chemistry and Biochemistry, Queens College of the City University of New York, 65 30 Kissena Boulevard, Flushing, NY 11367, USA
    J Biol Chem 279:36462-9. 2004
    ..Our observations suggest that psoralen cross-links can be repaired by three pathways: an error-free recombinational pathway requiring NER and HR and two PRR-dependent error-prone pathways, one NER-dependent and one NER-independent...
  81. ncbi Strategies for covalent attachment of DNA to beads
    Gali Steinberg
    Illumina, Inc, 9885 Towne Centre Drive, San Diego, CA 92121, USA
    Biopolymers 73:597-605. 2004
    ..We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads...
  82. ncbi Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: a model for the structure of the PIC
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue North, Seattle, WA 98109 USA
    Cell 119:169-80. 2004
    ..The TFIIF subunit Tfg1 was found in close proximity to the TFIIB B finger, linker, and core domains, suggesting that these two factors closely cooperate during initiation...
  83. ncbi Transglutaminase-catalyzed cross-linking of osteopontin is inhibited by osteocalcin
    M T Kaartinen
    Department of Biochemistry and Biotechnology, University of Kuopio, FIN 70211 Kuopio, Finland
    J Biol Chem 272:22736-41. 1997
    ..This suggests a specific role and a plausible mechanism for it as a modulator of maturation, stabilization, and calcification of bone matrix...
  84. pmc Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 99:3475-80. 2002
    ..The findings confirm previous observations indicating that intermolecular cross-linking is a stochastic process resulting from random collisions and support a number of other lines of evidence that lactose permease is a monomer...
  85. ncbi RNA-tethered phenyl azide photocrosslinking via a short-lived indiscriminant electrophile
    Karen L Buchmueller
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Am Chem Soc 125:10850-61. 2003
    ..RNA-tethered 3-hydroxyphenyl azide cross-linking in aqueous buffer can thus be used with confidence to map structural neighbors, including most dynamic interactions, in RNA...
  86. ncbi HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
    Peter Cherepanov
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
    J Biol Chem 278:372-81. 2003
    ....
  87. ncbi Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters
    Stefan Kalkhof
    Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin Luther University Halle Wittenberg, Wolfgang Langenbeck Strasse 4, 06120 Halle Saale, Germany
    Anal Bioanal Chem 392:305-12. 2008
    ..This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry...
  88. ncbi Mass spectrometry identifiable cross-linking strategy for studying protein-protein interactions
    Xiaoting Tang
    Department of Chemistry, Washington State University, Pullman, WA 99164 4630, USA
    Anal Chem 77:311-8. 2005
    ..In addition, dead-end, intra-, and inter-cross-linked peptides can be distinguished by analyzing MS/MS spectra...
  89. pmc Mismatch repair participates in error-free processing of DNA interstrand crosslinks in human cells
    Qi Wu
    Department of Carcinogenesis, University of Texas M D Anderson Cancer Center, Science Park Research Division, 1808 Park Road 1 C, Smithville, Texas 78957, USA
    EMBO Rep 6:551-7. 2005
    ..Thus, enhancement of MMR relative to NER may reduce the mutagenesis caused by DNA ICLs in humans...
  90. ncbi Identification of protein-protein interactions using in vivo cross-linking and mass spectrometry
    Julian Vasilescu
    The Biomedical Research Centre, University of British Columbia, Vancouver, Canada
    Proteomics 4:3845-54. 2004
    ..Among these, we identified the RasGAP-related protein IQGAP1 to be a novel interaction partner of M-Ras(Q71L). This method is applicable to many proteins and will aid in the study of protein-protein interactions...
  91. ncbi Molecular characterization of the principal substrate binding site of the ubiquitous folding catalyst protein disulfide isomerase
    Annamari Pirneskoski
    Biocenter Oulu and Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 279:10374-81. 2004
    ....
  92. ncbi Psoralen photocrosslinking, a tool to study the chromatin structure of RNA polymerase I--transcribed ribosomal genes
    Martin Toussaint
    Department de Microbiologie et Infectiologie, Universite de Sherbrooke, QC, Canada
    Biochem Cell Biol 83:449-59. 2005
    ..In this review we focused on the studies of chromatin in ribosomal genes and how these results have helped to address the fundamental question: What is the structure of chromatin in the coding regions of genes?..
  93. ncbi Utility of formaldehyde cross-linking and mass spectrometry in the study of protein-protein interactions
    Brent W Sutherland
    Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    J Mass Spectrom 43:699-715. 2008
    ....
  94. ncbi Orientation of the crotonaldehyde-derived N2-[3-Oxo-1(S)-methyl-propyl]-dG DNA adduct hinders interstrand cross-link formation in the 5'-CpG-3' sequence
    Young Jin Cho
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, USA
    Chem Res Toxicol 19:1019-29. 2006
    ....
  95. ncbi Drosophila homologs of FANCD2 and FANCL function in DNA repair
    Lorri R Marek
    Department of Genetics, Yale University School of Medicine, 333 Cedar St, New Haven, CT 06520 8005, USA
    DNA Repair (Amst) 5:1317-26. 2006
    ..Because of the lack of other FA genes, further studies will determine whether the conserved FA genes function as the minimal machinery or whether additional genes are involved in the Drosophila FA pathway...
  96. pmc Nonprotein based enrichment method to analyze peptide cross-linking in protein complexes
    Funing Yan
    Department of Biochemistry, and Biodefense Proteomics Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Anal Chem 81:7149-59. 2009
    ..These features may make the method suitable to investigate protein-protein interactions in biological samples...
  97. ncbi New chemical crosslinking methods for the identification of transient protein-protein interactions with multiprotein complexes
    K Melcher
    Institut fur Mikrobiologie, Biozentrum, Goethe Universitat, Marie Curie St 9, N250, 60439 Frankfurt am Main, Germany
    Curr Protein Pept Sci 5:287-96. 2004
    ..Methods discussed fall into two categories: (i) in vitro approaches with localized, inducible crosslinking reagents and (ii) in vivo approaches with unlocalized crosslinkers...
  98. pmc Double-strand breaks induce homologous recombinational repair of interstrand cross-links via cooperation of MSH2, ERCC1-XPF, REV3, and the Fanconi anemia pathway
    Nianxiang Zhang
    Department of Cancer Genetics, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, United States
    DNA Repair (Amst) 6:1670-8. 2007
    ..These results suggest that the juxtaposition of an ICL and a DSB stimulates repair of ICLs through a process requiring components of mismatch repair, ERCC1-XPF, REV3, Fanconi anemia proteins, and homologous recombination repair factors...
  99. ncbi Isotope-tagged cross-linking reagents. A new tool in mass spectrometric protein interaction analysis
    D R Muller
    Functional Genomics, Novartis Pharma AG, Basel, Switzerland
    Anal Chem 73:1927-34. 2001
    ..This novel methodology allowed us to significantly refine the current view of the complex between Op18 and tubulin corroborating the tubulin "capping" activity of the N-terminal domain of Op18...
  100. ncbi Photocrosslinkers illuminate interactions in living cells
    Yoshihito Tanaka
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Mol Biosyst 4:473-80. 2008
    ..We also describe how these methods offer a non-invasive way to study macromolecular interactions in a native context...
  101. ncbi Function of a eukaryotic transcription activator during the transcription cycle
    James Fishburn
    Howard Hughes Medical Institute, 1100 Fairview Ave North, Mailstop A1 162, Seattle, Washington 98109, USA
    Mol Cell 18:369-78. 2005
    ..Thus, a single activating region contacts multiple factors, and each contact makes differential contributions to transcriptional activation...

Research Grants62

  1. Structural Biology of the Apical Bile Acid Transporter.
    Peter W Swaan; Fiscal Year: 2012
    ....
  2. Mechanisms of eukaryotic transcription activation
    Steven M Hahn; Fiscal Year: 2013
    ....
  3. DEFINING RESIDUES CRITICAL TO FIBRIN CLOT STRUCTURE
    Susan T Lord; Fiscal Year: 2010
    ..Data obtained from these studies will lead to a more complete understanding of the events that are critical for an effective clot structure, which will likely improve our ability to diagnose and treat thrombotic disease. ..
  4. Thwarting HIV evasion of antibody avidity with novel antibody architectures
    Pamela J Bjorkman; Fiscal Year: 2012
    ..The trimeric reagent's intra-spike cross-linking would reduce the concentration required for sterilizing immunity, making HIV's low spike ..
  5. Thwarting HIV evasion of antibody avidity with novel antibody architectures
    Pamela J Bjorkman; Fiscal Year: 2013
    ..The trimeric reagent's intra-spike cross-linking would reduce the concentration required for sterilizing immunity, making HIV's low spike ..
  6. Molecular Studies of Human CNS Glutamate Transporters
    Gonzalo E Torres; Fiscal Year: 2013
    ..abstract_text> ..
  7. AVIDIN-BIOTIN TECHNOLOGY FOR HORMONE RECEPTOR ISOLATION
    Klaus Hofmann; Fiscal Year: 1990
    ..Noteworthy is the fact that we have been successful in solubilizing the AII receptor with retention of binding affinity for I251-labeled biotinylated AII probes...
  8. MOLECULAR MECHANISMS OF INDUCED IMMUNE CYTOLYSIS
    Carl Wilhelm Vogel; Fiscal Year: 1990
    ..Finally, monoclonal antibodies to CVF will be used as a tool to identify immunogenic epitopes and functional sites on CVF, cobra C3, and human C3 as an approach to study the structure/function relationships of these homologous proteins...
  9. NOVEL IMMUNOCONJUGATE APPROACHES
    Carl Wilhelm Vogel; Fiscal Year: 1990
    ..In vivo properties of the conjugates to be analyzed include the stability, plasma half- life, and biodistribution in normal mice...
  10. DEVELOPMENT OF IMMUNOTOXINS USING A MUTANT OF EXOTOXIN A
    Robert Fulton; Fiscal Year: 1991
    ..The successful attainment of the objectives of this research will eventually make immunotoxins with increased cytotoxic potency commercially available both for research purposes and for the therapy of human disease...
  11. ANTIMALARIAL ACTION AND RESISTANCE
    Donald Krogstad; Fiscal Year: 1991
    ..The broad long-term goal of these studies is to provide a rational basis for the development of antimalarials active against multiply-resistant P. falciparum...
  12. ANALYSIS OF MOTOR PROTEIN FUNCTION
    Edward Pate; Fiscal Year: 2004
    ..Although this approach will be shown to be a two-way street, the most significant advantage clearly accrues to the much less studied microtubule motor family. ..
  13. IMIDOESTERS AS ANTISICKLING AGENTS
    David Albert; Fiscal Year: 1991
    ....
  14. STRUCTURE-FUNCTION STUDIES ON MICROSOMAL MEMBRANES
    PHILIPP STRITTMATTER; Fiscal Year: 1992
    ....
  15. Microsphere Coatings for Protein Arrays
    PATRICK GUIRE; Fiscal Year: 2007
    ..Additionally, the improved microarrays will benefit researchers studying protein-protein interactions which may ultimately greatly aid in disease management. [unreadable] [unreadable] [unreadable]..
  16. BIOCHEMICAL CHARACTERIZATION OF THE AH RECEPTOR
    Gary Perdew; Fiscal Year: 1993
    ..The experiments proposed here will enable a better understanding of possible mechanisms of Ah receptor regulation...
  17. Immunoglobulin Uptake in Ticks
    Algimantas Jasinskas; Fiscal Year: 2005
    ..The location and temporal expression of the IBPs will be further characterized by tissue immunofluorescence. ..
  18. CHROMATIN CHANGES DURING IMMUNOGLOBULIN GENE ACTIVATION
    Jesse Summers; Fiscal Year: 1990
    ....
  19. APOB FOLDING & SECRETION--CHARACTERIZING HELPER PROTEINS
    Haya Herscovitz; Fiscal Year: 2000
    ..Two categories will be defined: a) known protein/s with a novel function or b) novel protein/s of unknown sequence. The mode of their interaction with apo B will be studied. ..
  20. COMPLEMENT AND CANCER--MOLECULAR AND THERAPEUTIC
    Carl Wilhelm Vogel; Fiscal Year: 1990
    ..This approach would result in a target-specific, non-toxic and non-immunogenic cytotoxic agent...
  21. MOLECULAR RULERS FOR RIBOSOMES
    Eric Wickstrom; Fiscal Year: 1980
    ..Fragments which are labeled due to alkylation by labeled molecular rulers may be identified by amino acid compositions, which will be determined by HPLC of fluorescently modified amino acids following hydrolysis...
  22. FACTORS CONTROLLING PROTEIN SYNTHESIS
    JOANNE RAVEL; Fiscal Year: 1990
    ..Deoxyoligonucleotides complementary to sequences in the mRNAs will be used to determine what regions of the mRNA are involved in the recognition and/or binding of the initiation factors and in the hydrolysis of ATP...
  23. Mechanism and Inhibition of Herpes Replication
    ROBERT KUCHTA; Fiscal Year: 2002
    ..Additionally, a number of novel nucleotides will be synthesized to provide a thorough understanding of how the enzyme interacts with the incoming NTP. ..
  24. IMMUNOSENESCENCE AND ALTERED GERMINAL CENTER RESPONSE
    Biao Zheng; Fiscal Year: 2003
    ..These approaches will make possible to systemically study the effect of aging on germinal center response and its consequence. ..
  25. NONMUSCLE ALPHA-ACTININ & ACTIN-MEMBRANE INTERACTION
    Keith Burridge; Fiscal Year: 1980
    ..Through the use of cleavable, chemical crosslinking reagents we hope to determine the order of proteins linking actin to the membrane. ..
  26. ANTIBODY PROBES OF CNS MITOCHONDRIAL APOPTOTIC FUNCTION
    Ronald Jemmerson; Fiscal Year: 2004
    ..The feasibility of the proposed research is supported by preliminary results and by the participation of on-site collaborators with expertise in the field of neuroscience, in particular the physiology of CNS mitochondria. ..
  27. DEVELOPMENT OF RNA FRIENDLY, HISTOLOGICAL FIXATIVES
    URSULA WINZER SERHAN; Fiscal Year: 1999
    ..PROPOSED COMMERCIAL APPLICATIONS. Improvements to current technologies will allow high quality RNA to be isolated from histologically preserved samples. Ambion will develop and market this methodology in kit form. ..
  28. ACQUISITION OF A MALDI MASS SPECTROMETER
    John Allison; Fiscal Year: 1999
    ..abstract_text> ..
  29. ORGANIZATION AND BIOSYNTHESIS OF ER MEMBRANE PROTEINS
    Gert Kreibich; Fiscal Year: 1991
    ..Analysis of the structure of the genes for these membrane proteins should ultimately provide information on the regulatory elements involved in the coordinate expression of the components of the translocation apparatus...
  30. MEMBRANE-MICROFILAMENT CONNECTIONS
    JOHN GLENNEY; Fiscal Year: 1991
    ..These studies will further our understanding of the subcortical filament network and its relationship to cell transformation...
  31. NMDA receptors with restricted mobility of the ligand binding domain
    Gabriela Popescu; Fiscal Year: 2009
    ....
  32. Qualitative Analysis of Tissue Biomarkers and Pathways
    Robert Camp; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  33. PROTEIN-PROTEIN INTERACTIONS IN E. COLI RIBOSOMES
    JEFFREY COHLBERG; Fiscal Year: 1980
    ..Other studies are aimed at exploring the utility of certain bifunctional reagents in covalently cross-linking ribosomal proteins to ribosomal RNA, to aid in locating the RNA binding sites of various proteins. ..
  34. CONTRACTILE PROTEINS FROM SMOOTH MUSCLE CELLS
    PETER RUBENSTEIN; Fiscal Year: 1980
    ..In particular, we will seek to determine if there is a requisite order for the introduction of these modifications, and we will attempt to evaluate the role of N-acetylation in actin function...
  35. Cross-linked Polymer Micelles in Cancer Therapy
    Tatiana K Bronich; Fiscal Year: 2010
    ..It is anticipated that these studies will lead to the design of new formulation of cisplatin for improved cancer treatment. ..
  36. 9th International Symposium on the Maillard Reaction
    Vincent Monnier; Fiscal Year: 2007
    ..The Proceedings of these symposia are always published: the Proceedings of the 8th International Maillard symposium appearing as Vol. 1043 of the Annals of the New York Academy of Sciences. [unreadable] [unreadable] [unreadable]..
  37. Metal-Mediated Routes to Biaryls
    Bruce Lipshutz; Fiscal Year: 2007
    ..A particular, albeit representative, application of a novel substituted cyclo-NOBIN will be studied for selected asymmetric aldol reactions. ..
  38. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2009
    ..Understanding the role and mechanism of axon atrophy in solvent neurotoxicity has broad-based implications for human occupational health and risk assessment. ..
  39. Catalytic Processes for Selective Organic Synthesis
    GARY MOLANDER; Fiscal Year: 2005
    ..4) Carbometalation/cyclization reactions will be explored utilizing organochromium species. 5) Ligands and catalytic systems designed for asymmetric synthesis will be explored. ..
  40. FINE TUNING BINOLS AND BINAPS FOR ASYMMETRIC SYNTHESIS
    Bruce Lipshutz; Fiscal Year: 2001
    ..From ligands of this type come further options, such as opportunities for polymer mounting, and potentially of greatest attention, conversion to substituted cyclo BINAPs. ..
  41. COMBINATORIAL PHARMACOTHERAPIES FOR COCAINE DEPENDENCE
    John Cashman; Fiscal Year: 2001
    ..The study will provide an understanding of the structural and pharmaceutical properties of hDAT antagonists. The work will lead to new insight into the preparation of human medications necessary in the cessation of cocaine abuse. ..
  42. New Opioids for Alcoholism
    John Cashman; Fiscal Year: 2006
    ..The potential commercial application of the work is that the research could lead to a 'blockbuster' drug product. [unreadable] [unreadable]..
  43. Agilent 5973 GC/MS System
    GARY MOLANDER; Fiscal Year: 2002
    ..Our current lack of such instrumentation negatively affects the ability to carry out many of these studies. This critical capability, in the form of an instrument for everyday analysis, is urgently needed. ..
  44. Amine N-Oxygenation by FMO3 and FMO4
    John Cashman; Fiscal Year: 2005
    ..Such fundamental information will be useful in the development of safer drugs, the prevention of adverse drug reactions and the protection of humans from disease. ..
  45. COLLAGEN CROSSLINKING BY THE MAILLARD REACTIOIN IN AGING
    Vincent Monnier; Fiscal Year: 2004
    ..Our approach, we expect, will result in the first comprehensive, hierarchical map of collagen crosslinks that for, during aging. ..
  46. MECHANICS OF ACUTE LUNG INJURY
    Jason Bates; Fiscal Year: 2009
    ..The results of our studies are expected to lead to the design of physiologically-based lung recruitment strategies that can be tailored to the individual patient with ALI. This will set the stage for future clinical trials. ..
  47. Targeted Correction of the Human Beta-globin Gene
    Peter M Glazer; Fiscal Year: 2010
    ..Our long-range goal is to develop such molecules as reagents for targeted genome modification of disease-related genes in human cells. ..
  48. MOLEC GENETICS OF COMPLEX I IN MAMMALIAN MITOCHONDRIA
    Immo Scheffler; Fiscal Year: 2004
    ..Such mice promise to become model systems for mitochondrial diseases. 5) Known cDNAs will be tested to identify the defective gene in the other four complementation groups with complex I defects. ..
  49. AUTOCRINE ROLE OF GASTRIN-LIKE PEPTIDES IN COLON CANCER
    Pomila Singh; Fiscal Year: 2002
    ..If we are successful in purifying the novel GP-R (and confirm its role in mediating the mitogenic effects of gastrins) then one can develop systemic strategies to block the receptor mediated effects in future funding periods. ..
  50. Assessment of Lung function in Mice
    Jason Bates; Fiscal Year: 2009
    ....
  51. Gene expression in memory CD4+T cells.
    Henry Wong; Fiscal Year: 2006
    ..abstract_text> ..
  52. Targeting Amyloid Intermediates by Design and Selection
    Indraneel Ghosh; Fiscal Year: 2006
    ..The ultimate objective is to correlate the various physical, kinetic and lexicological parameters obtained in these experiments to propose rational strategies for treating AD. ..
  53. Purchase of an ion trap mass spectrometer
    David Speicher; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. Eukaryotic Transcription Factor Binding Site Locations
    Martha Bulyk; Fiscal Year: 2007
    ..In addition, we will make our data publicly available, so that other researchers may focus their efforts on those genomic regions most likely to contain cis regulatory elements. ..
  55. Light activated bioactive substrate release
    RICHARD GIVENS; Fiscal Year: 2008
    ..abstract_text> ..
  56. MECHANISMS OF RECOMBINATIONAL DNA REPAIR
    Randy Legerski; Fiscal Year: 2005
    ..abstract_text> ..
  57. TECHNOLOGIES FOR IDENTIFYING PROTEINS SECRETED BY TUMORS
    David Speicher; Fiscal Year: 2005
    ..nanoscale in-gel digestion method; 3) Compare the 1-D gel pixelation approach with MudPIT analyses using prefractionated serum; and 4) Produce and systematically evaluate serum samples from SCID mice with ovarian tumors ..
  58. SYNTHESIS OF ANTICANCER AGENTS
    K C Nicolaou; Fiscal Year: 2010
    ..abstract_text> ..
  59. Comparative Mechanisms of Genomic Instability
    Karen M Vasquez; Fiscal Year: 2010
    ..These discoveries should begin to unravel the pathogenesis of diseases that are caused by genomic instability, and ultimately to the development of new approaches for treatment and prevention. ..