indicators and reagents


Summary: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)

Top Publications

  1. ncbi A simple sample preparation with HPLC-UV method for estimation of tiropramide from plasma: application to bioequivalence study
    Khan Imran
    Department of Pharmacology, The Bombay College of Pharmacy, Kalina, Santacruz E, Mumbai 400 098, India
    J Pharm Biomed Anal 43:1135-40. 2007
  2. pmc Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish
    Akira Muto
    Division of Molecular and Developmental Biology, National Institute of Genetics, Graduate University for Advanced Studies, SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 108:5425-30. 2011
  3. ncbi The green fluorescent protein
    R Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
    Annu Rev Biochem 67:509-44. 1998
  4. ncbi Comparison of alamar blue and MTT assays for high through-put screening
    R Hamid
    Discovery Support, Purdue Pharma L P, 444 Saw Mill River Road, Ardsley, NY 10502, USA
    Toxicol In Vitro 18:703-10. 2004
  5. ncbi Multi-platform, multi-site instrumentation and reagent standardization
    N Purvis
    Cytometry Associates, Inc, Brentwood, Tennessee 37027, USA
    Cytometry 33:156-65. 1998
  6. pmc The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
    P S Maddox
    Department of Biology, CB3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Nat Cell Biol 2:36-41. 2000
  7. ncbi Broad-range ribosomal RNA real-time PCR after removal of DNA from reagents: melting profiles for clinically important bacteria
    Ching Ping Tseng
    School of Medical Technology and Graduate Institute of Medical Biotechnology, Chang Gung University, Tao Yuan 333, Taiwan, Republic of China
    Clin Chem 49:306-9. 2003
  8. ncbi Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein
    M M Mogensen
    Department of Anatomy and Physiology, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 113:3013-23. 2000
  9. ncbi Intracellular internalization mechanism of protein transfection reagents
    Makoto Oba
    Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852 8521, Japan
    Biol Pharm Bull 35:1064-8. 2012
  10. ncbi Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
    James D Hirsch
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Anal Biochem 308:343-57. 2002

Detail Information

Publications279 found, 100 shown here

  1. ncbi A simple sample preparation with HPLC-UV method for estimation of tiropramide from plasma: application to bioequivalence study
    Khan Imran
    Department of Pharmacology, The Bombay College of Pharmacy, Kalina, Santacruz E, Mumbai 400 098, India
    J Pharm Biomed Anal 43:1135-40. 2007
    ..These results indicated that the analytical method was linear, precise and accurate. Test and reference formulation were found to be bioequivalent...
  2. pmc Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish
    Akira Muto
    Division of Molecular and Developmental Biology, National Institute of Genetics, Graduate University for Advanced Studies, SOKENDAI, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 108:5425-30. 2011
    ..We propose the GCaMP technology combined with the Gal4FF-UAS system is a powerful tool to study functional neural circuits in zebrafish...
  3. ncbi The green fluorescent protein
    R Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
    Annu Rev Biochem 67:509-44. 1998
    ..Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories...
  4. ncbi Comparison of alamar blue and MTT assays for high through-put screening
    R Hamid
    Discovery Support, Purdue Pharma L P, 444 Saw Mill River Road, Ardsley, NY 10502, USA
    Toxicol In Vitro 18:703-10. 2004
  5. ncbi Multi-platform, multi-site instrumentation and reagent standardization
    N Purvis
    Cytometry Associates, Inc, Brentwood, Tennessee 37027, USA
    Cytometry 33:156-65. 1998
    ..Data are presented that illustrates why standardization is needed and how we have attempted to achieve it in our laboratories...
  6. pmc The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
    P S Maddox
    Department of Biology, CB3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Nat Cell Biol 2:36-41. 2000
    ..These results show for astral and anaphase interpolar spindle microtubules, and possibly for metaphase spindle microtubules, that microtubule assembly and disassembly occur at plus, and not minus, ends...
  7. ncbi Broad-range ribosomal RNA real-time PCR after removal of DNA from reagents: melting profiles for clinically important bacteria
    Ching Ping Tseng
    School of Medical Technology and Graduate Institute of Medical Biotechnology, Chang Gung University, Tao Yuan 333, Taiwan, Republic of China
    Clin Chem 49:306-9. 2003
  8. ncbi Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein
    M M Mogensen
    Department of Anatomy and Physiology, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 113:3013-23. 2000
    ..We propose that ninein is a non-nucleating microtubule minus-end associated protein which may have a dual role as a minus-end capping and anchoring protein...
  9. ncbi Intracellular internalization mechanism of protein transfection reagents
    Makoto Oba
    Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852 8521, Japan
    Biol Pharm Bull 35:1064-8. 2012
    ..It is our hope that the results presented here will help in the choice and use of protein transfection reagents for experiments...
  10. ncbi Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
    James D Hirsch
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Anal Biochem 308:343-57. 2002
    ..Thus, our desthiobiotin-based reagents and techniques provide some distinct advantages over traditional 2-iminobiotin, monomeric avidin, or other affinity-based techniques...
  11. ncbi Staining of proteins for 2D SDS-PAGE using Coomassie Blue--speed versus sensitivity?
    Pavel Májek
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Electrophoresis 34:1972-5. 2013
    ..PLoS One 2011, 6, e22394) seems to be less sensitive than colloidal Coomassie staining when the number of detected spots is the evaluating criterion. No obvious influence of gel boiling on the protein identification was observed...
  12. pmc A new reagent for direct difluoromethylation
    Yuta Fujiwara
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 134:1494-7. 2012
    ..Regiochemical comparisons suggest that the CF(2)H radical generated from the new reagent possesses nucleophilic character...
  13. ncbi Anatomical and functional outcome in brilliant blue G assisted chromovitrectomy
    Paul B Henrich
    Department of Ophthalmology, University Hospital Basel, Switzerland
    Acta Ophthalmol 88:588-93. 2010
    ..To evaluate the potential of brilliant blue G (BBG) for intraoperative staining of the inner limiting membrane (ILM) with respect to staining properties and surgical outcome...
  14. pmc Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene
    A F Norman
    Department of Microbiology, University of Georgia, Athens, USA
    J Clin Microbiol 33:1797-803. 1995
    ..henselae strain. Current studies are aimed at optimization of PCR conditions for specificity and sensitivity of amplification of Bartonella sequences from clinical isolates...
  15. ncbi Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents
    Philip L Ross
    Applied Biosystems, Framingham, MA 01701, USA
    Mol Cell Proteomics 3:1154-69. 2004
    ..We find that inactivation of Upf1p and Xrn1p causes common as well as unique effects on protein expression...
  16. ncbi Influence of neutral protease activity on human islet isolation outcome
    H Brandhorst
    Third Medical Department, University Hospital, Giessen, Germany
    Transplant Proc 37:241-2. 2005
    ..Since no information is available about the effect of NP activity on islet release from the human pancreas, the present study evaluated the effect of various NP concentrations on the outcome of human islet isolation...
  17. ncbi Amplification of ST50 gene using dry-reagent-based polymerase chain reaction for the detection of Salmonella typhi
    Ismail Aziah
    Institute for Research in Molecular Medicine, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
    Diagn Microbiol Infect Dis 59:373-7. 2007
    ..9%, 100%, 100%, and 90.9%, respectively. We suggest that this rapid 2-step PCR test could be used for the rapid diagnosis of typhoid fever...
  18. ncbi Continuous pH monitoring in a perfused bioreactor system using an optical pH sensor
    Antony S Jeevarajan
    Wyle Laboratories, Life Sciences Systems and Services, 1290 Hercules Drive, Suite 120, Mail Stop BT 37, Houston, Texas 77058, USA
    Biotechnol Bioeng 78:467-72. 2002
    ..06 pH unit and for the fiber optically coupled Shimadzu spectrophotometer against the blood gas analyzer was +/-0.05 pH unit. The pH sensor system performed well without need of recalibration for 124 days...
  19. ncbi Peptide chiral purity determination: hydrolysis in deuterated acid, derivatization with Marfey's reagent and analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry
    D R Goodlett
    Immunobiology Research Institute, Annandale, NJ 08801, USA
    J Chromatogr A 707:233-44. 1995
    ..This procedure was used to determine the chiral purity of each amino acid in a purified, hexapeptide by-product (Arg-Lys-Lys-Asp-Val-Tyr) present in a kilogram batch of the synthetic pentapeptide, thymopentin (Arg-Lys-Asp-Val-Tyr)...
  20. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
  21. ncbi Screening for colorectal cancer: randomised trial comparing guaiac-based and immunochemical faecal occult blood testing and flexible sigmoidoscopy
    L Hol
    Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Centre Rotterdam, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Gut 59:62-8. 2010
    ..We conducted a randomised trial comparing participation and detection rates (DR) per screenee of guaiac-based faecal occult blood test (gFOBT), immunochemical FOBT (FIT), and flexible sigmoidoscopy (FS) for CRC screening...
  22. ncbi Comparative toxicology of trypan blue, brilliant blue G, and their combination together with polyethylene glycol on human pigment epithelial cells
    Doaa Awad
    Department of Chemistry, University of Bremen, Bremen, Germany
    Invest Ophthalmol Vis Sci 52:4085-90. 2011
  23. ncbi No evidence of murine-like gammaretroviruses in CFS patients previously identified as XMRV-infected
    Konstance Knox
    Wisconsin Viral Research Group, Milwaukee, WI 53226, USA
    Science 333:94-7. 2011
    ..Consistent with previous reports, we detected MLV sequences in commercial laboratory reagents. Our results indicate that previous evidence linking XMRV and MLVs to CFS is likely attributable to laboratory contamination...
  24. pmc Steric factors influencing hybridisation of nucleic acids to oligonucleotide arrays
    M S Shchepinov
    Department of Biochemistry, University of Oxford, South Parks Road Oxford OX1 3QU, UK
    Nucleic Acids Res 25:1155-61. 1997
    ..Surface coverage was varied using a combination of cleavable and stable linkers, giving the highest hybridisation yields for surfaces containing approximately 50% of the maximum concentration of oligonucleotides...
  25. ncbi Simple reagents for direct halonium-induced polyene cyclizations
    Scott A Snyder
    Department of Chemistry, Columbia University, Havemeyer Hall, 3000 Broadway, New York, New York 10027, USA
    J Am Chem Soc 132:14303-14. 2010
    ..Preliminary attempts to use chiral forms of the reagent class for enantioselective alkene halogenation are also described...
  26. pmc Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology
    Michael I Kotlikoff
    Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853 6401, USA
    J Physiol 578:55-67. 2007
    ..Finally, newer strategies for effective genetic specification of GECIs are briefly reviewed...
  27. ncbi Decontamination of polymerase chain reaction reagents using DEAE-cellulose
    S A Glushkov
    Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russian Federation
    Anal Biochem 393:135-7. 2009
    ..We also show the suitability of the proposed approach for decontamination of the Taq polymerase, monoclonal antibodies to Taq polymerase, and Moloney murine leukemia virus (M-MLV) reverse transcriptase...
  28. ncbi Development and validation of a high-throughput double solid phase extraction-liquid chromatography-tandem mass spectrometry method for the determination of tetrodotoxin in human urine and plasma
    Bonnie Mei Wah Fong
    Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, HKSAR, PR China
    Talanta 83:1030-6. 2011
    ..The creatinine-adjusted TTX concentration in urine (ranging from 7.4 to 41.1 ng μmol(-1) creatinine) correlated well with the degree of poisoning as observed from clinical symptoms...
  29. pmc Possible mechanism of miltefosine-mediated death of Leishmania donovani
    Navin K Verma
    Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Punjab, India
    Antimicrob Agents Chemother 48:3010-5. 2004
    ..Understanding of miltefosine-mediated death will facilitate the design of new therapeutic strategies against Leishmania parasites...
  30. ncbi Technical, experimental, and biological variations in isobaric tags for relative and absolute quantitation (iTRAQ)
    Chee Sian Gan
    Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom
    J Proteome Res 6:821-7. 2007
    ..These findings underline the importance of replicate analysis as a validation tool and benchmarking technique in protein expression analysis...
  31. ncbi Reagent decontamination to eliminate false-positives in Escherichia coli qPCR
    Sarah S Silkie
    Civil and Environmental Engineering Department, University of California, Berkeley, CA 94720 1710, USA
    J Microbiol Methods 72:275-82. 2008
    ..5 microL of master mix). Both decontamination methods could eliminate up to 100 copies of added contaminant DNA and did not cause PCR inhibition, resulting in a reduction of the detection limit to 10 copies per reaction well...
  32. ncbi Comparative evaluation of anatomical and functional outcomes using brilliant blue G versus triamcinolone assisted ILM peeling in macular hole surgery in Indian population
    Atul Kumar
    Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India
    Graefes Arch Clin Exp Ophthalmol 249:987-95. 2011
    ..To compare anatomical and functional outcomes using brilliant blue G (BBG) vs triamcinolone acetonide (TA)-assisted ILM peeling in macular hole surgery (MHS)...
  33. pmc Copper-mediated difluoromethylation of aryl and vinyl iodides
    Patrick S Fier
    Department of Chemistry, University of California, Berkeley, California 94720, United States
    J Am Chem Soc 134:5524-7. 2012
    ..This transformation is surprising, in part, because of the prior observation of the instability of CuCF(2)H...
  34. ncbi Cervical cancer screening using visual inspection with acetic acid: operational experiences from Ghana and Thailand
    Harshad Sanghvi
    JHPIEGO, Baltimore MD, USA
    Reprod Health Matters 16:67-77. 2008
    ..However, this study shows that a single-visit approach with VIA and cryotherapy is programmatically feasible and sustainable and should be considered in national investments to control cervical cancer...
  35. pmc Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba
    James McBride
    Department of Immunology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Scotland, UK
    J Clin Microbiol 43:629-34. 2005
    ..As it is based on a standard 96-well, microtiter plate, it is amenable to automation and high throughput...
  36. ncbi A rapid resazurin bioassay for assessing the toxicity of fungicides
    Patricia Bi Fai
    Centre for Ecology, Evolution and Conservation CEEC, School of Environmental Sciences, University of East Anglia, Norwich NR47TJ, UK
    Chemosphere 74:1165-70. 2009
    ..It will be valuable for screening chemical libraries for fungicides and as a biomarker for detecting the effects of fungicides to non-target fungi...
  37. pmc iTRAQ reagent-based quantitative proteomic analysis on a linear ion trap mass spectrometer
    Timothy J Griffin
    Department of Biochemistry, Molecular Biology, and Biophysics, Center for Mass Spectrometry and Proteomics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Proteome Res 6:4200-9. 2007
    ..Thus, we demonstrate the effectiveness of LTQ-PQD analyzing iTRAQ reagent-labeled peptides, and provide guidelines for successful quantitative proteomic studies...
  38. ncbi Effect of visual screening on cervical cancer incidence and mortality in Tamil Nadu, India: a cluster-randomised trial
    Rengaswamy Sankaranarayanan
    Screening Group, International Agency for Research on Cancer, Lyon, France
    Lancet 370:398-406. 2007
    ..We assessed the effect of screening using visual inspection with 4% acetic acid (VIA) on cervical cancer incidence and mortality in a cluster randomised controlled trial in India...
  39. ncbi Selective determination of ammonium ions by high-speed ion-exclusion chromatography on a weakly basic anion-exchange resin column
    Masanobu Mori
    National Institute and Advanced Industrial Science and Technology at Seto, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:191-7. 2003
    ..3 min with a detection limit (S/N=3) better than 0.125 microM. Ammonium ion in rain and river waters was precisely determined using this ion-exclusion chromatographic system...
  40. ncbi Regioselective oxidative coupling of 4-hydroxystilbenes: synthesis of resveratrol and epsilon-viniferin (E)-dehydrodimers
    Magoichi Sako
    Gifu Pharmaceutical University, 5 6 1, Mitahora Higashi, Gifu 502 8585, Japan
    J Org Chem 69:2598-600. 2004
  41. ncbi Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations
    U Imeh
    Procter Department of Food Science, University of Leeds, LS2 9JT, UK
    J Agric Food Chem 50:6301-6. 2002
    ..The calculated dietary intake of total, free, and conjugated phenols from average per capita consumption of apples and pears in different regions of the U.K. varied between 104-126, 53-64, and 51-62 mg of CtE/day, respectively...
  42. ncbi Acid-triggered release via dePEGylation of DOPE liposomes containing acid-labile vinyl ether PEG-lipids
    Junhwa Shin
    Department of Chemistry, 560 Oval Drive, Purdue University, West Lafayette, IN 47907, USA
    J Control Release 91:187-200. 2003
    ..These studies also suggested that the presence of a slight negative charge at the membrane surface can accelerate the acid-catalyzed leakage process...
  43. pmc Chemical synthesis of a biologically active natural tRNA with its minor bases
    D Gasparutto
    , CIS Bio International, , France
    Nucleic Acids Res 20:5159-66. 1992
    ..The synthetic tRNA(Ala) has been transcribed into cDNA by reverse transcriptase and sequenced. With E. coli alanyl-tRNA synthetase the alanyl acceptance activity and kcat/Km were 672 pmol/A260 and 6 x 10(4)M-1s-1, respectively...
  44. ncbi Analysis of HIV-1 variants by cloning DNA generated with the ViroSeq HIV-1 Genotyping System
    Susan H Eshleman
    Department of Pathology, The Johns Hopkins Medical Institutions, Ross Bldg 646, 720 Rutland Ave, Baltimore, MD 21205, USA
    Biotechniques 35:614-8, 620, 622. 2003
  45. ncbi The study of dynamic chemical magnifying endoscopy in gastric neoplasia
    Kazuyoshi Yagi
    Department of Internal Medicine, Niigata Prefectural Yoshida Hospital, Yoshida machi, Niigata ken, Japan
    Gastrointest Endosc 62:963-9. 2005
    ..We assessed the usefulness of acetic acid-enhanced magnifying endoscopy in the diagnosis of gastric neoplasia...
  46. ncbi Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo
    T Greener
    Laboratory of Cell Biology, NHLBI, NIH, Bethesda, MD 20892 0301, USA
    Nat Cell Biol 3:215-9. 2001
    ..We conclude that auxilin is required for in vivo clathrin-mediated endocytosis and development in C. elegans...
  47. ncbi Gating dependence of inner pore access in inward rectifier K(+) channels
    L Revell Phillips
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110, USA
    Neuron 37:953-62. 2003
  48. pmc Flexibility of the Kir6.2 inward rectifier K(+) channel pore
    G Loussouarn
    Departments of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 98:4227-32. 2001
    ..2 model this narrowing excludes all ions. To allow entry of ions as large as MTSPTrEA(+) or qBBr(+), the entrance must widen to >8 A, but this widening is readily accomplished by minimal M2 helix motion and side-chain rearrangement...
  49. ncbi An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury
    J S Sun
    Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, Republic of China
    Burns 24:225-31. 1998
  50. ncbi mDia mediates Rho-regulated formation and orientation of stable microtubules
    A F Palazzo
    Department of Anatomy and Cell Biology, Columbia University, New York 10032, USA
    Nat Cell Biol 3:723-9. 2001
    ..Our results show that mDia is sufficient to generate and orient stable microtubules, and indicate that Dia-related formins are part of a conserved pathway that regulates the dynamics of microtubule ends...
  51. ncbi Selection of antibodies against biotinylated antigens
    Patrick Chames
    Department of Pathology, Maastricht University and University Hospital, Maastricht, The Netherlands
    Methods Mol Biol 178:147-57. 2002
  52. ncbi Connexin-GFPs shed light on regulation of cell-cell communication by gap junctions
    Vytas K Verselis
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Curr Drug Targets 3:483-99. 2002
  53. ncbi Evaluation of accuracy and uncertainty of ELISA assays for the determination of interleukin-4, interleukin-5, interferon-gamma and tumor necrosis factor-alpha
    Lone Borg
    Reference Laboratory, National Institute of Occupational Health, Copenhagen, Denmark
    Clin Chem Lab Med 40:509-19. 2002
    ..The model for evaluating uncertainty presented in this paper is applicable to other types of assays and to most analytical methods...
  54. ncbi [High dose intravitreal brilliant blue G]
    E Cervera
    Arch Soc Esp Oftalmol 82:473. 2007
  55. ncbi Microfluidic combinatorial chemistry
    Paul Watts
    Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, UK
    Curr Opin Chem Biol 7:380-7. 2003
    ..One of the immediate and obvious applications is therefore in combinatorial chemistry and drug discovery...
  56. pmc Probing the pore of ClC-0 by substituted cysteine accessibility method using methane thiosulfonate reagents
    Chia Wei Lin
    Center for Neuroscience, University of California Davis, CA 95616, USA
    J Gen Physiol 122:147-59. 2003
    ..Thus, the proposal that the glutamate side chain is the fast gate of the channel is applicable to ClC-0, revealing a structural and functional conservation of ClC channels between bacterial and vertebrate species...
  57. ncbi pH fluorosensors for use in marine systems
    Claudia R Schröder
    University of Regensburg, Institute of Analytical Chemistry, Chemo and Biosensors, 93040 Regensburg, Germany
    Analyst 130:907-16. 2005
    ..This type of sensor can be applied for pH imaging or in phase-modulation measurements of pH...
  58. ncbi Endoscopic instillation of indigo carmine dye with acetic acid enables the visualization of distinct margin of superficial gastric lesion; Usefulness in endoscopic treatment and diagnosis of gastric cancer
    H Yamashita
    Dig Liver Dis 39:389-91. 2007
  59. pmc 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment for Alzheimer's disease
    Leila Choe
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA
    Proteomics 7:3651-60. 2007
    ..One conclusion aided by the visualization tool is that there are differences in considering peptide-based observations versus protein-based observations from quantitative shotgun proteomics studies...
  60. pmc A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore
    Galen E Flynn
    Department of Physiology and Biophysics, University of Washington, Seattle 98195 7290, USA
    J Gen Physiol 121:563-82. 2003
  61. ncbi Building addressable libraries: the use of electrochemistry for generating reactive Pd(II) reagents at preselected sites on a chip
    Eden Tesfu
    Department of Chemistry, Washington University, St Louis, Missouri 63130, and CombiMatrix Corporation, 6500 Harbor Heights Parkway, Suite 301, Mukilteo, Washington 98275, USA
    J Am Chem Soc 126:6212-3. 2004
  62. ncbi HPLC determination of malondialdehyde in ECV304 cell culture medium for measuring the antioxidant effect of vitexin-4''-O-glucoside
    Xi Xiang Ying
    School of Pharmacy, Liaoning University of TCM, No 79 Chongshan East Road, Shenyang, China
    Arch Pharm Res 31:878-85. 2008
    ..0125 microM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4''-O-glucoside. Vitexin-4''-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP...
  63. ncbi LC determination of glimepiride and its related impurities
    Mubeen Ahmad Khan
    Analytical Research Lab, Glenmark Research Centre, Plot A 607, TTC Industrial Area, MIDC, Mahape, 400709 Navi Mumbai, India
    J Pharm Biomed Anal 39:928-43. 2005
    ..The method has been validated as per international guidelines on method validation and can be used for the routine quality control analysis of glimepiride as active pharmaceutical ingredient (API)...
  64. ncbi Catecholase activity of a mu-hydroxodicopper(II) macrocyclic complex: structures, intermediates and reaction mechanism
    Iryna A Koval
    Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300, RA, Leiden, Netherlands
    J Biol Inorg Chem 10:739-50. 2005
    ..A catalytic mechanism of catechol oxidation by 1 has been proposed, and its relevance to the mechanisms earlier proposed for the natural enzyme and other copper complexes is discussed...
  65. ncbi Liquid chromatographic method for quantitation of patulin at 10 ng/mL in apple-based products intended for infants: interlaboratory study
    Isabel Arranz
    Institute for Reference Materials and Measurements, Food Safety and Quality Unit, European Commission Joint Research Centre, Retieseweg 111, B 2440 Geel, Belgium
    J AOAC Int 88:518-25. 2005
    ..5 to 35.2%, reflecting HORRAT values from 0.6 to 1.0 for juice and 0.4 to 0.9 for puree. The method showed acceptable within-laboratory and between-laboratory precision for each matrix, as required by current European legislation...
  66. ncbi Identification of antitumour activity of novel derivatives of 8-aryl-2,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3,4-dione and 8-aryl-4-imino-2,3,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazin-3(6H)-one
    Krzysztof Sztanke
    Chair and Department of Medical Chemistry, Professor Feliks Skubiszewski Medical University, 4 Staszica Street, 20 081 Lublin, Poland
    Bioorg Med Chem 15:2837-49. 2007
    ..Six of them (1-2, 4-6 and 9) proved active as antimicrobials. All these compounds revealed MIC values in the range of 15.0-78.6 microM. Their activities were compared to those of ampicillin and chloramphenicol...
  67. ncbi Synthesis of 6beta-D-glucosyl and 6-nitroxy (-)-galanthamine derivatives as acetylcholinesterase inhibitors
    E Perissutti
    Dipartimento di Chimica Farmaceutica e Tossicologica1, Universita di Napoli Federico II, Napoli, Italy
    Pharmazie 62:403-5. 2007
    ..Compounds 2, 3 and 5 presented a percentage of inhibition of 35.22%, 47.48% and 67.89% respectively...
  68. ncbi Enantioselective analysis of the metabolites of hydroxychloroquine and application to an in vitro metabolic study
    Carmem Dickow Cardoso
    Departamento de Quimica, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto USP, Av Bandeirantes 3900, 14040 901 Ribeirao Preto, Sao Paulo, Brazil
    J Pharm Biomed Anal 37:703-8. 2005
    ..The metabolic study demonstrated that metabolism is stereoselective for HCQ. The major metabolites formed in the incubation of racemic HCQ were (-)-(R)-DCQ and (-)-(R)-DHCQ with R/S ratios of 2.2 and 3.3, respectively...
  69. ncbi HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum
    Xiao Ming Wang
    Shanghai Research Center for TCM Modernization, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Guo Shoujing Road 199, Zhangjiang, Shanghai, PR China
    J Pharm Biomed Anal 43:1185-90. 2007
    ..Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time...
  70. ncbi Optimization and determination of the absolute configuration of a series of potent inhibitors of human papillomavirus type-11 E1-E2 protein-protein interaction: a combined medicinal chemistry, NMR and computational chemistry approach
    Nathalie Goudreau
    Department of Chemistry, Boehringer Ingelheim Canada Ltd, Research and Development, 2100 Cunard Street, Laval, Que, Canada H7S 2G5
    Bioorg Med Chem 15:2690-700. 2007
    ..In addition, we report a combined NMR and computational chemistry approach which allowed the successful determination of the absolute stereochemistry of the active species originating from the initial racemic lead...
  71. ncbi Quantification of eugenol, luteolin, ursolic acid, and oleanolic acid in black (Krishna Tulasi) and green (Sri Tulasi) varieties of Ocimum sanctum Linn. using high-performance thin-layer chromatography
    Sheetal Anandjiwala
    B V Patel Pharmaceutical Education and Research Development Centre, Pharmacognosy and Phytochemistry Department, Thaltej, Ahmedabad 380 054, Gujarat, India
    J AOAC Int 89:1467-74. 2006
    ..The HPTLC-densitometry methods for the quantification of the 4 markers in O. sanctum leaf will have the applicability in quality control...
  72. ncbi Synthesis of DL-standishinal and its related compounds for the studies on structure-activity relationship of inhibitory activity against aromatase
    Takahiro Katoh
    Department of Pharmaceutical Manufacturing Chemistry, 21st Century COE Program, Kyoto Pharmaceutical University, 1 Shichono cho, Misasagi, Yamashina ku, Kyoto 607 8412, Japan
    Bioorg Med Chem 15:2736-48. 2007
  73. ncbi Synthesis of the four stereoisomers of 2,6-dimethyloctane-1,8-dioic acid, a component of the copulation release pheromone of the cowpea weevil, Callosobruchus maculatus
    Tomonori Nakai
    Department of Fermentation Science, Faculty of Applied Biological Science, Tokyo University of Agriculture, Japan
    Biosci Biotechnol Biochem 69:2401-8. 2005
    ..The stereoisomeric purities of the four synthetic isomers of 2 were determined by the HPLC analyses of their bis-2-(2,3-anthracenedicarboximide)-1-cyclohexyl esters...
  74. ncbi A simple high-performance liquid chromatographic method for the determination of acyclovir in human plasma and application to a pharmacokinetic study
    Liyan Yu
    Center for Instrumental Analysis, China Pharmaceutical University, Key Laboratory of Drug Quality Control and Pharmacovigilance under the Ministry of Education, Nanjing People s Republic of China
    Arzneimittelforschung 58:199-202. 2008
    ..90% confidence limits calculated for C(max) and AUC from zero to infinity (AUC(0-infinity)) of acyclovir were included in the bioequivalence range (0.8-1.25 for AUC)...
  75. ncbi Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine
    M D Arbo
    Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmacia, Universidade Federal do Rio Grande do Sul, Av Ipiranga, 2752, CEP 90610 000, Porto Alegre, RS, Brazil
    Food Chem Toxicol 46:2770-5. 2008
    ..All the effects were reversible and persisted for 3-4h. The toxic effects observed seem to be related with adrenergic stimulation and should alert for possible side effects of p-synephrine and C. aurantium...
  76. ncbi Stereoselective syntheses of highly functionalized bicyclo[3.1.0]hexanes: a general methodology for the synthesis of potent and selective mGluR2/3 agonists
    Lushi Tan
    Department of Process Research, Merck Research Laboratories, P O Box 2000, Rahway, New Jersey 07065, USA
    J Org Chem 70:8027-34. 2005
    ..Application of this reaction to the synthesis of mGluR2/3 agonist 1 (43% overall yield) and a few intermediates suitable for the synthesis of other bicyclo[3.1.0]hexane mGluR2/3 agonists is discussed...
  77. ncbi Enantiospecific assay of citadiol--a key intermediate of escitalopram by liquid chromatography on Chiralpak AD-H column connected with UV and polarimetric detectors in series
    R Nageswara Rao
    HPLC UV Group, Division of Analytical Chemistry, Discovery Laboratory, Indian Institute of Chemical Technology, Hyderabad 500007, India
    J Pharm Biomed Anal 43:311-4. 2007
    ..1-300 microg/ml for R-CTD and S-CTD, respectively and the r2 was > or = 0.9999. The inter- and intra-day assay precision was less than 0.74% (%R.S.D.) and the recoveries were in the range 99.68-100.72% with %R.S.D. < 0.49%...
  78. ncbi Synthesis of highly condensed polycyclic carbohydrates by reaction of a spirocyclic enamino sulfonate derived from d-xylofuranose with bifunctional reagents
    Alessandra Cordeiro
    Instituto de Quimica Medica CSIC, Juan de la Cierva 3, 28006 Madrid, Spain
    J Org Chem 72:9713-21. 2007
    ..X-ray diffraction analysis and intensive NMR studies with one of these carbohydrates were performed to highlight the strained nature of these compounds...
  79. ncbi alpha-Biphenylsulfonylamino 2-methylpropyl phosphonates: enantioselective synthesis and selective inhibition of MMPs
    Alessandro Biasone
    Dipartimento di Scienze del Farmaco, Universita G D Annunzio, Via dei Vestini 31, 66013 Chieti, Italy
    Bioorg Med Chem 15:791-9. 2007
    ..Screening of the new compounds on MMP-1, -2, -3, -7, -8, -9, -13 and -14 showed IC(50) in the range of nM in most cases...
  80. ncbi Diastereomeric and enantiomeric high-performance liquid chromatographic separation of synthetic anisodamine
    Li Min Yang
    Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, South ChongQing Road 280, Shanghai 200025, China
    J Pharm Biomed Anal 43:905-9. 2007
    ..The methods were optimized by studying mobile phase modifiers, concentration of modifier and column temperature. The HPLC method for the simultaneous separation of two pairs of enantiomers of 654-2 has been validated...
  81. ncbi Simultaneous identification and quantification of anthraquinones, polydatin, and resveratrol in Polygonum multiflorum, various Polygonum species, and dietary supplements by liquid chromatography and microscopic study of Polygonum species
    Bharathi Avula
    National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA
    J AOAC Int 90:1532-8. 2007
    ..multiflorum. The commercial samples and the 6 Polygonum species were compared microscopically, and a detailed description is provided for P. multiflorum...
  82. ncbi Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography
    Hossein Amini
    Department of Pharmacology, Neuroscience Research Center, Shaheed Beheshti Medical University, Tehran, Iran
    J Pharm Biomed Anal 43:1146-50. 2007
    ..125-2 microg/ml for TMP and 0.39-50 microg/ml for SMX. The limit of quantification (LOQ) in plasma was 0.125 and 0.39 microg/ml for TMP and SMX, respectively. The method was used for a bioequivalence study...
  83. ncbi Enantiodiscrimination by NMR spectroscopy
    Gloria Uccello-Barretta
    Dipartimento di Chimica e Chimica Industriale, Universita di Pisa, Via Risorgimento 35, 56126 Pisa, Italy
    Curr Pharm Des 12:4023-45. 2006
  84. ncbi Direct asymmetric intermolecular aldol reactions catalyzed by amino acids and small peptides
    Armando Córdova
    Department of Organic Chemistry, The Arrhenius Laboratory, Stockholm University, 106 91 Stockholm, Sweden
    Chemistry 12:5383-97. 2006
    ..These results suggest that the prebiotic oligomerization of amino acids to peptides may plausibly have been a link in the evolution of the homochirality of sugars. The mechanism and stereochemistry of the reactions are also discussed...
  85. ncbi High performance liquid chromatographic determination of etofibrate and its hydrolysis products
    Alaa El-Gindy
    Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
    J Pharm Biomed Anal 43:196-203. 2007
    ..01 M sodium hydroxide at different temperatures cannot be studied as the drug is rapidly hydrolyzed in alkaline medium. The pH-rate profile of hydrolysis of EF in Britton-Robinson buffer solutions within the pH range 2-10 were studied...
  86. pmc Detection of DNA adducts derived from the reactive metabolite of furan, cis-2-butene-1,4-dial
    Michael C Byrns
    Division of Environmental Health and Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Chem Res Toxicol 19:414-20. 2006
    ..These data support the hypothesis that cis-butene-1,4-dial is a genotoxic metabolite of furan. This method will allow us to explore the role of these adducts in furan-induced carcinogenesis...
  87. ncbi Preparative method of R-(-)-lbuprofen by diastereomer crystallization
    Tran Quoc Trung
    College of Pharmacy, Kangwon National University, Chunchon 200 701, Korea
    Arch Pharm Res 29:108-11. 2006
    ..R-(-)-ibuprofen was recovered producing overall yield of 2.4% with the purity more than 99.97%...
  88. ncbi Verticilide: elucidation of absolute configuration and total synthesis
    Souichi Monma
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, Tokyo 108 8641, Japan
    Org Lett 8:5601-4. 2006
    ..We also describe the practical total synthesis of verticilide. [reaction: see text]...
  89. ncbi Construction of quaternary stereocenters by efficient and practical conjugate additions to alpha,beta-unsaturated ketones with a chiral organic catalyst
    Fanghui Wu
    Department of Chemistry, Brandeis University, Waltham, MA 02454 9110, USA
    Angew Chem Int Ed Engl 45:947-50. 2006
  90. ncbi Determination of absolute configurations by X-ray crystallography and 1H NMR anisotropy
    Nobuyuki Harada
    Department of Chemistry, Columbia University, New York 10027, USA
    Chirality 20:691-723. 2008
    ..In this review, the principles and applications of these X-ray and NMR methods are explained using mostly our own data...
  91. ncbi Vitamin B12: a methyl group without a job?
    Philip Butler
    Institute of Organic Chemistry, Innrain 52a, Leopold Franzens Universitat Innsbruck, Austria
    Angew Chem Int Ed Engl 45:989-93. 2006
  92. ncbi Development and validation of a simple HPLC method for simultaneous in vitro determination of amoxicillin and metronidazole at single wavelength
    Naser Tavakoli
    Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
    J Pharm Biomed Anal 43:325-9. 2007
    ..13 microg/ml for metronidazole. The determination of the two active ingredients was not interfered by the excipients of the products. Samples were stable in the release media (37 degrees C) and the HPLC injector at least for 12 h...
  93. ncbi Determination of the new HIV-protease inhibitor atazanavir by liquid chromatography after solid-phase extraction
    S Colombo
    Division de pharmacologie clinique, Departement de Medecine, Laboratoire BH 18 218, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, CHUV, Switzerland
    J Chromatogr B Analyt Technol Biomed Life Sci 810:25-34. 2004
    ..4 +/- 3.2%. The method is precise with mean inter-day CVs within 1.1-6.1%, and accurate (range of inter-day deviations +0.3 to +2.3%). The method has been validated and is currently applied to the monitoring of ATV in HIV patients...
  94. ncbi [Isomeric phenanthridines from 1,2-dihydro-5-methyl-2'-nitro-[1,1'-biphenyl]-2,6-dicarboxylic acid esters]
    K Görlitzer
    Institut für Pharmazeutische Chemie der Technischen Universität Braunschweig, Germany
    Pharmazie 58:776-87. 2003
    ..The cyclic hydroxamic acids 13 and 14a are tested for their ability to inhibit 5-, 12- and 15-lipoxygenase. Compound 13 represents a weak inhibitor of 5-lipoxygenase in human whole blood...
  95. ncbi Identification of potent and novel small-molecule inhibitors of caspase-3
    Darin A Allen
    Sunesis Pharmaceuticals, Inc, 341 Oyster Point Boulevard, South San Francisco, CA 94080, USA
    Bioorg Med Chem Lett 13:3651-5. 2003
    ..The design and synthesis of a series of novel, reversible, small molecule inhibitors of caspase-3 are described...
  96. ncbi Double diastereoselective intramolecular cyclopropanation to P-chiral [3.1.0]-bicyclic phosphonates
    Joel D Moore
    Department of Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045 7582, USA
    Org Lett 4:2357-60. 2002
    ..This approach is being developed as an effective method to access bicyclic P-chiral phosphonates...
  97. ncbi Spectrophotometric and liquid chromatographic determination of trimebutine maleate in the presence of its degradation products
    Alaa El-Gindy
    Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
    J Pharm Biomed Anal 33:231-41. 2003
    ..The proposed HPLC and 1D methods were used to investigate the kinetics of acidic and alkaline degradation processes. The pH-rate profile of degradation of TM in Britton-Robinson buffer solutions within the pH range 2-11.9 was studied...
  98. ncbi Stereospecific high-performance liquid chromatographic analysis of flurbiprofen: application to pharmacokinetic studies
    Xiao Wei Teng
    Department of Pharmaceutical Sciences and Pharmacology, College of Pharmacy, Washington State University, P O Box 646534, Pullman, WA 99164 6534, USA
    J Pharm Biomed Anal 33:95-100. 2003
    ..6% at the limit of quantitation (LOQ) (0.05 microg/ml). Bias of the assay was lower than 13.1%, and was within 12.8% at the LOQ. The assay was applied successfully to the in vivo kinetic study of flurbiprofen in rats...
  99. ncbi Simultaneous determination of terpene lactones and flavonoid aglycones in Ginkgo biloba by high-performance liquid chromatography with evaporative light scattering detection
    Wenkui Li
    Department of Medicinal Chemistry and Pharmacognosy, Program for Collaborative Research in Pharmaceutical Sciences, University of Illinois at Chicago, 833 South Wood Street, 60612 7231, Chicago, IL, USA
    J Pharm Biomed Anal 30:67-75. 2002
    ..998. The reproducibility of the method was evaluated by analyzing three sets of controls on 3 consecutive days with RSD% and relative errors (RE%) less than 17.26 and 14.67%...
  100. ncbi Chromatographic determination of clotrimazole, ketoconazole and fluconazole in pharmaceutical formulations
    E M Abdel-Moety
    Faculty of Pharmacy, Cairo University, Kasr El Aini, ET 11562 Cairo, Egypt
    Farmaco 57:931-8. 2002
    ..1, by volumes), CZ was well separated from its acid degradation products and quantified by densitometric scanning at 260 nm...
  101. ncbi The enantiospecific, stereospecific total synthesis of the ring-A oxygenated sarpagine indole alkaloids (+)-majvinine, (+)-10-methoxyaffinisine, and (+)-N(a)-methylsarpagine, as well as the total synthesis of the alstonia bisindole alkaloid macralstonidin
    Shuo Zhao
    Department of Chemistry, University of Wisconsin Milwaukee, Milwaukee, Wisconsin 53201, USA
    J Org Chem 68:6279-95. 2003
    ..This approach to ring-A substituted alkoxyindole alkaloids should find wide application for the synthesis of other alkaloids for it is stereospecific and either enantiomer can be prepared with ease...

Research Grants82

  1. The Primate Corpus Luteum: Functional Regression and Cardiovascular Impacts
    Randy L Bogan; Fiscal Year: 2012
    ..abstract_text> ..
  2. Brain Activation responses in adolescents with genetic predisposition to obesity
    Susan Carnell; Fiscal Year: 2011
    ..The results may lead to new drugs and interventions to improve eating behavior, and help establish unbiased indicators of obesity risk and prognosis for use in research and clinical settings. ..
    DHAN V KALVAKOLANU; Fiscal Year: 2013
    ..Studies proposed in this application will investigate the regulation of an anti-metastatic gene, DAPK1, which is critical for tumor suppression and autophagic responses. ..
  4. Cre, Dre Dual Lineage Model for Calcium Imaging and Optogenetic Manipulations
    Petr Tvrdik; Fiscal Year: 2013
    ..We will be able to determine if Hoxb8 mutant microglia respond to neural stimuli correctly. Taken together, the proposed work will advance the set of tools available to address the underlying causes of glial and other disorders. ..
  5. Phage Display for Improved Peptide-based Tumor Targeting and Imaging Agents
    Susan L Deutscher; Fiscal Year: 2013
    ..This work is significant and relevant to the VA mission because the peptides discovered here may translate into novel prostate, breast, and ovarian cancer diagnostics and therapeutics for our veterans. ..
  6. Predictors of Pregnancy Outcome in SLE and APS
    Jane E Salmon; Fiscal Year: 2012
  7. Disease chronicity in juvenile dermatomyositis (JDM): Epigenetic clues
    Lauren M Pachman; Fiscal Year: 2013
    ..The goal is to identify specific markers, like microRNA, or "turned on genes" that may be key to repair the vascular damage and to identify more effective medical interventions. ..
  8. Novel SCID rat models for human cell transplantation studies
    Joseph C Ruiz; Fiscal Year: 2010
    ..There is a compelling need for an abundant and accessible supply of animals to screen for new therapeutic agents. Thus, if successful, this project would benefit many goals of public health. ..
  9. Role of TPH2 and 5HT Neuronal Loss in Non-motor Symptoms of Parkinson's
    Donald M Kuhn; Fiscal Year: 2012
  10. Study of zidovudine addition in HIV-associated neurocognitive disorders
    Albert Anderson; Fiscal Year: 2013
    ..Overall, this career development project will allow the principal investigator to pursue the goal o becoming an established investigator and leader in HIV CNS research. ..
  11. Multivariate CART for survival with dental applications
    Martha E Nunn; Fiscal Year: 2013
  12. Exploring persistent epigenetic changes after prenatal famine exposure in humans
    L H Lumey; Fiscal Year: 2010
    ..The required medical examination records and biological materials for this study have been previously collected and stored and pending funding will be immediately available for analysis. ..
    David R Jacobs; Fiscal Year: 2013
    David Jacobs; Fiscal Year: 2009
    ..abstract_text> ..
    PALMER WILLIAM TAYLOR; Fiscal Year: 2013
  16. Diet Quality and CVD Risk Factors in a Family Based Weight Management Study
    Alice H Lichtenstein; Fiscal Year: 2013
    ..S., a public health problem that threatens to overwhelm our healthcare system. (End of Abstract) ..
  17. Climate Change and Adverse Birth Outcomes: Assessing the Vulnerability of Pregnan
    Shao Lin; Fiscal Year: 2013
    ..The multidisciplinary research team has a unique opportunity to use the data already collected and geo-coded through the NBDPS and other ongoing projects, which ensures the project is feasible and sustainable. ..
  18. MRI of Acute Vascular Injury and Hemorrhagic Transformation in Ischemic Stroke
    Robert A Knight; Fiscal Year: 2010
    ..e., BBB opening) and both will be functions of the variations in BBB associated proteins and/or brain swelling;and 2) the size of these changes are correlated over time, ROI, and treatment. ..
  19. Oxidative stress and neurogenetic networks in Drosophila
    Robert R H Anholt; Fiscal Year: 2010
  20. Right Heart-Pulmonary Vascular Interactions in Bronchopulmonary Dysplasia
    Marlowe Eldridge; Fiscal Year: 2013
    ..We have a unique and important opportunity to investigate the consequences of BPD on lung and right heart function in this population, which will inform treatment strategies and predictions of disease progression in the future. ..
  21. Food(cassava) cyanogen exposure and motor neuron degeneration
    ..This project has a global heath relevance because the crop (cassava) is a staple for more than 400 million people dwelling under the tropics and it is increasingly exported worldwide for snack production and animal feed. ..
  22. Amoeboid Membrane Dynamics in Prostate Cancer
    Michael R Freeman; Fiscal Year: 2013
    ..DRF3 loss and the MAT may also be functionally important in other solid tumor systems. Consequently, these studies may provide information of more general significance to human cancer. ..
  23. Daily Experience in Adolescence and Biomarkers of Early Risk for Adult Health
    Andrew J Fuligni; Fiscal Year: 2013
    ..An understanding of these processes is necessary for the development of ethnically and culturally relevant prevention efforts. ..
  24. Depression, Adipocytokines and Metabolic Dysregulation in Black and White Women
    Susan A Everson Rose; Fiscal Year: 2010
    ..Results of the study will provide significant new information on how depression affects risk for diabetes, metabolic syndrome, obesity and cardiovascular disease in women. ..
  25. Perinatal Programming of Infant Stress Reactivity and the Atopic Phenotype
    Rosalind J Wright; Fiscal Year: 2013
    ..Given the enormous cost in the management of atopic patients in the United States, understanding the earliest stages of development offers significant potential benefits to society. ..
  26. microRNAs as novel biomarkers for management of breast cancer
    LORENZO SEMPERE; Fiscal Year: 2012
    ..Our results should provide a solid foundation to develop miRNA-based clinical assays to assist physicians in making crucial decisions with regards to treatment of patients. ..
  27. Metagenomic profiling of oral polymicrobial flora in head and neck cancers
    Charis Eng; Fiscal Year: 2011
    ..Importantly, even for advanced HNSCC or that which has spread, our findings will help point to antibiotics and agents that can turn back on the cancer-suppressing genes to revolutionize treatment. ..
  28. Quality Dialysis Outcomes and Practice Patterns Study (DOPPS): high-quality care
    Francesca Tentori; Fiscal Year: 2013
    ..abstract_text> ..
  29. CTRIP: Molecular phenotypes of rapidly progressive idiopathic pulmonary fibrosis
    Galen B Toews; Fiscal Year: 2010
  30. Retinal Imaging of Prognostic Indicators of Atherosclerosis
    Frederick R Haselton; Fiscal Year: 2010
    ..Proven biomarker correlates will then be imaged in vivo in a mouse model of atherosclerosis to validate the utility of our imaging approach. ..
    Veronique L Roger; Fiscal Year: 2012
    ..It will determine the importance of novel biomarkers to predict the risk of adverse events in clinical practice. ..
  32. The Effect of Behavioral and Physiological Factors on Obesity Risk in Children
    Karen B Dorsey; Fiscal Year: 2012
    ..abstract_text> ..
  33. Improving Quality of Care in Parkinsons Disease: A Randomized Controlled Trial
    KAREN ILENE CONNOR; Fiscal Year: 2013
  34. Exploring Human-Induced Pluripotent Stem Cells for Alzheimer's Disease Research
    Kuo Fen Lee; Fiscal Year: 2012
    ..Ultimately, we aim to develop genetic or chemical treatments that disrupt molecular pathways that lead to AD. ..
  35. A simple dietary message to improve dietary quality for metabolic syndrome
    Yunsheng Ma; Fiscal Year: 2012
    ..We hypothesize that a message promoting high fiber intake may be the simple message that has potential to produce broad impact. ..
  36. Mechanisms of Cell Death after Photodynamic Therapy
    Anna Liisa Nieminen; Fiscal Year: 2010
    ..The proposed studies will enhance our understanding how to enhance PDT-induced killing of cancer cells and further increase the efficacy of PDT translation to the benefit for cancer patients. ..
    Bertil Hille; Fiscal Year: 2013
    ..All quantitative results will be analyzed by an integrated realistic explanatory kinetic model for neurons and expression cells. ..
  38. Effect of Diet on Inflammation in Sicke Cell Mice
    Jacqueline M Hibbert; Fiscal Year: 2010
    ..The use of this mouse model is important for investigating the time course of sickle cell induced tissue inflammation, and for seeking practical and effective dietary treatment to reduce the tissue injury. ..
  39. Prenatal Cocaine Exposure: Young Adult Follow Up
    Ruth Rose-Jacobs; Fiscal Year: 2013
  40. Risk for Dysfunctional Romantic Relationships for Adults
    Deborah M Capaldi; Fiscal Year: 2013
  41. Proteolytic Pathways in Thrombus Resolution
    Toni M Antalis; Fiscal Year: 2013
    ..The objectives of this basic research proposal are therefore of direct relevance to a serious condition that impacts the health of the warfighter, the military family, and the American public. ..
  42. Air pollution and the brain: gender as an important determinant of susceptibility
    Lucio G Costa; Fiscal Year: 2013
  43. Environmental Endocrine Disruptors and Human Sperm Chromosomal Abnormalities
    Melissa Perry; Fiscal Year: 2012
    ..This study investigates whether chemicals common in our daily environment can affect male fertility and potential risks for birth defects. ..
  44. Stress and Subclinical Cardio-Metabolic Disease in Police: A Longitudinal Study
    John M Violanti; Fiscal Year: 2013
  45. Pathogenesis/Treatment-Inherited Cholesterol Deficiency
    Gordon L Watson; Fiscal Year: 2013
    ..There is no cure, and current treatment of symptoms is inadequate. We are attacking this problem directly by using gene therapy to replace the defective gene that is the root cause of SLOS. ..
  46. Neural Actions of Toluene
    John J Woodward; Fiscal Year: 2013
    ..Research to be carried out in this proposal will provide a detailed analysis of the effects of abused inhalants on neurons within brain areas involved in addiction. ..
  47. Discovering tissue-specific biomarkers of radiation injury in SILAC-labeled mice
    Amanda G Paulovich; Fiscal Year: 2013
  48. Urine biomarkers of lupus nephritis pathology and response to therapy
    James C Oates; Fiscal Year: 2013
    ..The long-term goal is to provide clinicians with a qualified biomarker panel coupled with a web-based predictive tool for more accurate diagnosis and therapeutic monitoring of LN. ..
  49. RCT of Stress Reduction in the Secondary Prevention of CHD in African Americans
    Robert Schneider; Fiscal Year: 2010
    GEORGIRENE VLADUTIU; Fiscal Year: 2012
    ..abstract_text> ..
  51. The role of VEGF-C in resisting stress in prostate cancer
    Kaustubh Datta; Fiscal Year: 2013
    ..This study should provide potential targets for effective therapeutic interventions as well as prognostic indicators for the metastatic stage of prostate cancer. ..
  52. Mechanisms Underlying the Progression of Arterial Stiffness in Hypertension
    Jay D Humphrey; Fiscal Year: 2013
    ..abstract_text> ..
  53. MRI Imaging Biomarkers of ARPKD Kidney and Liver Disease
    Christopher A Flask; Fiscal Year: 2013
    ..The animal MRI imaging studies in this project are highly translatable to human ARPKD, and will provide a critical missing element needed for the development of clinical trials in ARPKD patients. ..
  54. Small Animal Model of HIV Neuropathogenesis in the HAART Era
    Pietro P Sanna; Fiscal Year: 2012
    ..Ultimately, the proposed Tg mice will allow mechanistic studies aimed at the generation of new pathogenetic hypotheses of HIV-associated neurocognitive disorders in the setting of HAART and their interaction with drugs of abuse. ..
  55. Cognitive Behavioral Therapy for Insomnia in Stable Heart Failure
    Nancy S Redeker; Fiscal Year: 2010
    ..We plan to test the effects of cognitive behavioral therapy, on sleep, daytime symptoms, and function middle-aged and older adults who have heart failure. ..
  56. An Integrated Study of Mitochondrial Pathways in Colorectal Cancer
    Bharat Thyagarajan; Fiscal Year: 2013
  57. Protein biomarkers of pulmonary hypertension in sickle cell disease
    Mark E McComb; Fiscal Year: 2013
  58. The Association of Chronic Exposure to Particulate Matter Air Pollutants, Inflamm
    RODRIGO XAVIER ARMIJOS; Fiscal Year: 2013
    ..The samples will be analyzed using an automated energy dispersive XRF instrument. . ..
  59. Sox10 Alleles for Functional Analysis of Glial Lineages
    E Michelle Southard-Smith; Fiscal Year: 2013
  60. Diagnostic Utility of mtDNA Content and Exercise Challenge in Veterans with GWI
    MICHAEL JOSEPH FALVO; Fiscal Year: 2013
    ..Additionally, the potential translational merits could be rapid as efficacious treatment is currently available for mitochondrial dysfunction. ..
  61. Measurement of Renal Viscoelastic Properties with Ultrasound
    MATTHEW WILLIAM URBAN; Fiscal Year: 2013
    ..3. Study the relationship between SDUV measurements of viscoelastic material properties with clinical measures for assessment of kidney transplant rejection. ..
  62. Impact of an Indoor Stove Intervention on Indicators of Cardiorespiratory Health
    Jennifer L Peel; Fiscal Year: 2012
    ..The proposed work will examine the impact of an improved indoor cook stove intervention on indicators of respiratory and cardiovascular health in a developing country. ..
  63. Etiology of breast development in the BCERC network: role of socioeconomic status
    DEJANA K BRAITHWAITE; Fiscal Year: 2010
    ..The Small Grants in Cancer Epidemiology Program mechanism provides an ideal vehicle to accomplish the objective of this study, which is aligned with the NCI's mission to determine the underlying causes of cancer health disparities. ..
  64. A Medical Home Pilot Evaluation: A Model for Comparative Effectiveness Research
    KIM DUNN DUNN; Fiscal Year: 2010
    ..Fourth, we will have a data set to compare safety net and private patients, treatments, and effectiveness to be used for comparative effectiveness research in community settings ..
  65. Non-Invasive Quantitation of Cellular and Biochemical Changes in Hereditary Ataxi
    GULIN OZ; Fiscal Year: 2009
    ..Successful application of such treatments is expected to reduce the burden caused by these diseases on individuals, their families and society. ..
  66. Bioengineering Studies of Abdominal Aortic Aneurysm Fluid and Wall Dynamics
    ENDER FINOL; Fiscal Year: 2009
    ..This methodology is expected to greatly enhance the presurgical planning capabilities of vascular surgeries and endovascular therapies in the future management of cardiovascular diseases. ..
  67. Does Enhanced School-Readiness Affect Adult Health of African Americans?
    Frances A Campbell; Fiscal Year: 2010
    ..The health care these adults provide for their own children will also be examined. ..
  68. Health, Pollution & Economic Development in South Durban
    Stuart Batterman; Fiscal Year: 2004
    ..The study is designed to respond to Durban's needs and serve as a model for other cities undergoing rapid urbanization and industrial development. ..
  69. Acoustic and Respiratory System Correlates of Pausing
    Janet Slifka; Fiscal Year: 2009
  70. Cholesterol Homeostasis in Framingham Offspring Study
    Alice Lichtenstein; Fiscal Year: 2006
    ..abstract_text> ..
    Andrew Leuchter; Fiscal Year: 1999
    ..Serial study will determine characteristics of lesions that adversely affect long-term prognosis, as well as the usefulness of QEEG for monitoring the evolution of white-matter disease. ..
    James Moe; Fiscal Year: 2009
  73. Dev. of a Suicide Prevention Training Curr f/Justice Sys Gatekeepers
    Henry J Steadman; Fiscal Year: 2009
    ..The potential for this project to have a significant impact on public health is paramount, as is its potential to act as a vehicle for creating, facilitating and integrating successful community partnerships . ..
  74. Validation of Measures of Restless Legs Syndrome for Elders with Memory Disorders
    Kathy Richards; Fiscal Year: 2009
    ..Receiver Operator Curves will determine the best single or combination of instruments to diagnose RLS. ..
    Dimitrios Trichopoulos; Fiscal Year: 2000
    ..Access to data of such superb quality will provide an important opportunity to critically evaluate the intrauterine origin of breast cancer hypothesis. ..
  76. Engineered Spores as Fluorogenic Biological Indicators for Sterility Testing
    Boris Rotman; Fiscal Year: 2009
    ..To provide more rapid sterility assurance, BCR Diagnostics has engineered unique living microorganisms that promptly (less than 30 min) send fluorescent light signals when the sterilization process is inadequate. ..
  77. Colonoscopy in Rural Oregon Practice (CROP)
    David Lieberman; Fiscal Year: 2009
    ..abstract_text> ..
    Peter Lee; Fiscal Year: 1999
    ..The results will provide a definition of fertility after cryptorchidism, provide a basis for guidelines not only for treatment of cryptorchidism, but for treatment and counseling of formerly cryptorchid men concerning fertility. ..
  79. Mathematical Modeling of Matrix Metalloproteinase-9 Driven Left Ventricular Remod
    Yufang Jin; Fiscal Year: 2010
  80. Preparation of Samples for Investigation of Biological Aging among the Tsimane
    Eileen Crimmins; Fiscal Year: 2009
    ..Results of this project will lead us to better understand the role of worldwide declines in infection throughout life in causing changes in the rate of aging in later life. ..
  81. Engineered Spores as Fluorogenic Biological Indicators for Sterility Testing
    Boris Rotman; Fiscal Year: 2007
    ..To provide more rapid sterility assurance, BCR Diagnostics has developed unique living microorganisms that promptly send fluorescent light signals when the sterilization process is inadequate. [unreadable] [unreadable] [unreadable]..
  82. Cambodia: Between the Khmers Rouges and the Red Plague?
    Patrick Heuveline; Fiscal Year: 2003
    ..The existence of this long-term observatory will complement and enhance future substantive research in this population. ..